##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.control.2d.S.1.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20729914
Total Bases	3.1 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.831280100824344	36.0	34.0	38.0	30.0	39.0
2	34.62085332336642	35.0	34.0	37.0	31.0	39.0
3	34.859114466176756	35.0	34.0	38.0	31.0	39.0
4	34.87102261977546	35.0	34.0	38.0	31.0	39.0
5	34.722585438608185	35.0	34.0	37.0	31.0	39.0
6	34.695301437333505	35.0	34.0	37.0	31.0	39.0
7	34.750452076163945	35.0	34.0	37.0	32.0	39.0
8	34.84997583685104	35.0	34.0	37.0	32.0	39.0
9	34.82388243385863	35.0	34.0	37.0	32.0	39.0
10-14	34.82028190758533	35.0	34.0	37.0	31.2	39.0
15-19	34.83093644286223	35.0	34.0	37.0	31.0	39.0
20-24	34.81832762065487	35.0	34.0	37.0	31.0	39.0
25-29	34.82262173398308	35.0	34.0	37.0	31.0	39.0
30-34	34.797843309914356	35.0	34.0	37.0	31.0	39.0
35-39	34.78830776625509	35.0	34.0	37.0	31.0	39.0
40-44	34.740354195391255	35.0	34.0	37.0	31.0	39.0
45-49	34.635795546474526	35.0	34.0	37.0	31.0	39.0
50-54	34.608817142222584	35.0	34.0	37.0	31.0	39.0
55-59	34.60079803514863	35.0	34.0	37.0	30.8	39.0
60-64	34.56928514995286	35.0	34.0	37.0	30.2	39.0
65-69	34.53899089981753	35.0	34.0	37.0	30.0	39.0
70-74	34.53690322111322	35.0	34.0	37.0	30.0	39.0
75-79	34.52111830275804	35.0	34.0	37.0	30.0	39.0
80-84	34.49824633136443	35.0	34.0	37.0	30.0	39.0
85-89	34.48872399567118	35.0	34.0	37.0	30.0	39.0
90-94	34.466220708875106	35.0	34.0	37.0	30.0	39.0
95-99	34.44312664297594	35.0	34.0	37.0	30.0	39.0
100-104	34.404634963753345	35.0	34.0	37.0	30.0	39.0
105-109	34.373680151302125	35.0	34.0	37.0	30.0	39.0
110-114	34.34746506907844	35.0	34.0	37.0	30.0	39.0
115-119	34.31400368568823	35.0	34.0	37.0	30.0	39.0
120-124	34.26758795043723	35.0	34.0	37.0	30.0	39.0
125-129	34.25043090868587	35.0	34.0	37.0	30.0	39.0
130-134	34.209245219251756	35.0	34.0	37.0	29.0	39.0
135-139	34.16652931604058	35.0	34.0	37.0	29.0	39.0
140-144	34.1215695443792	35.0	34.0	37.0	29.0	39.0
145-149	34.087134649955615	35.0	34.0	37.0	29.0	39.0
150	34.08167004455493	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	17.0
22	288.0
23	3121.0
24	19136.0
25	74846.0
26	186891.0
27	339963.0
28	496576.0
29	645602.0
30	803927.0
31	1004426.0
32	1275587.0
33	1667659.0
34	2286579.0
35	3523788.0
36	7172595.0
37	1228911.0
38	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.51659334575856	10.331571689092593	10.576372960455721	44.57546200469313
2	21.85047518567024	11.515725331389731	40.933888865921524	25.6999106170185
3	19.163301862159564	15.797917757638055	29.246215204714293	35.792565175488086
4	25.543725776823983	23.949530072254607	22.750097697098138	27.75664645382327
5	26.75080064407534	29.47299291220935	24.953225714407417	18.822980729307893
6	19.242253394073337	35.18026269803233	26.887090787044887	18.690393120849443
7	16.356927123105816	23.94585306530811	40.81867776432967	18.878542047256403
8	18.168269449470603	23.01919407842292	33.03848844684748	25.774048025258995
9	19.481766881204216	21.443150122576803	34.05973315786986	25.015349838349128
10-14	20.985765783119845	28.194279544140215	26.896383031990162	23.923571640749774
15-19	21.32345985449446	27.283383403964656	27.224637199252644	24.16851954228824
20-24	21.241101050731952	27.249107385562887	27.265988368937816	24.24380319476735
25-29	21.150463944929353	27.537100770316137	26.933301011508398	24.379134273246112
30-34	21.231626963329596	27.27307600110704	27.082788185769424	24.412508849793937
35-39	21.54617141825754	27.04703159668245	26.990050094440466	24.41674689061954
40-44	21.781288440925312	27.142778581234992	26.738002089739147	24.337930888100548
45-49	21.927113430794726	27.11677283098952	26.669892220488258	24.286221517727498
50-54	21.929760148946663	27.343956698815507	26.602207473342045	24.12407567889578
55-59	21.905683877297704	27.31491222742668	26.620502066557933	24.158901828717685
60-64	22.109736080504465	27.099735541186387	26.4151961887675	24.37533218954165
65-69	21.970459571889723	27.249828409899692	26.652449876897027	24.12726214131356
70-74	22.38331779617581	27.032388762595044	26.48107718932217	24.10321625190697
75-79	22.205753349049647	26.869519674227938	26.52398946741652	24.400737509305888
80-84	22.504137552424062	26.91643434028328	26.55248544415705	24.02694266313561
85-89	22.36668608017945	26.921284119837235	26.51730900579563	24.194720794187685
90-94	22.277818289341482	26.602773592033095	26.6027938526389	24.51661426598652
95-99	22.630540096802225	26.682096556687817	26.759131196757146	23.92823214975281
100-104	22.526761758116564	26.567662606955306	26.7624170682099	24.14315856671823
105-109	22.647391852155828	26.489221062370476	26.6202839814284	24.243103104045293
110-114	22.604619480876902	26.55979216567624	26.644646424764574	24.190941928682285
115-119	22.70387008278688	26.807645234110794	26.50077355886107	23.987711124241258
120-124	22.629666468897515	26.634470584430613	26.524318543509303	24.211544403162566
125-129	23.022509494767174	26.633903093813483	26.408031200470976	23.935556210948363
130-134	22.715210968425662	26.969961219789596	26.282687381605214	24.03214043017953
135-139	22.805385017672346	26.714716873410545	26.35537409528509	24.12452401363202
140-144	22.832539639261963	26.626914620762115	26.62033282396285	23.92021291601307
145-149	22.61271929666725	26.995745246751774	26.63779172128866	23.753743735292318
150	22.26950070646781	26.516074132568964	26.856616219381774	24.357808941581453
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.0
9	1.0
10	2.0
11	2.5
12	6.5
13	11.5
14	23.0
15	52.0
16	103.5
17	236.0
18	510.5
19	882.5
20	1369.5
21	2175.0
22	3508.0
23	5563.5
24	8772.5
25	13164.5
26	18466.0
27	26574.5
28	40062.0
29	58066.5
30	79575.5
31	101590.5
32	122794.0
33	149982.0
34	181213.5
35	220043.5
36	277571.0
37	343685.0
38	411763.5
39	504485.5
40	629255.5
41	769319.0
42	916892.0
43	1057439.0
44	1176908.0
45	1269754.0
46	1335172.0
47	1354200.5
48	1309896.0
49	1264410.0
50	1194965.5
51	1062058.0
52	901967.5
53	762817.0
54	638722.0
55	531949.0
56	463109.0
57	384898.5
58	317148.5
59	264464.0
60	205837.0
61	144582.5
62	84512.0
63	46412.0
64	29233.0
65	18518.5
66	12308.0
67	5981.5
68	2214.0
69	949.5
70	686.0
71	478.5
72	274.0
73	137.5
74	79.0
75	49.5
76	24.0
77	19.0
78	9.5
79	2.0
80	1.0
81	1.0
82	1.5
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.833099741754838E-4
2	8.200709371008486E-5
3	0.12488233188039276
4	1.2059866722071302E-4
5	1.9778181424196938E-4
6	1.398944539760271E-4
7	1.157747205318845E-4
8	1.4954234735368416E-4
9	2.6049312119674013E-4
10-14	2.3444380907706615E-4
15-19	3.0969737742279105E-4
20-24	3.8109178841745314E-4
25-29	2.961903266940712E-4
30-34	2.547043851701459E-4
35-39	1.389296646382614E-4
40-44	2.6049312119674013E-4
45-49	1.7945081682442098E-4
50-54	1.1480993119411881E-4
55-59	1.591902407313412E-4
60-64	1.5436629404251266E-4
65-69	0.04914250970843392
70-74	1.8813392086431232E-4
75-79	1.601550300691069E-4
80-84	1.2349303523401012E-4
85-89	1.119155631808217E-4
90-94	1.4664797934038702E-4
95-99	9.16549870877419E-5
100-104	8.007751503455344E-5
105-109	1.119155631808217E-4
110-114	8.490146172338196E-5
115-119	5.402820291487943E-5
120-124	1.0226766980316464E-4
125-129	1.0516203781646175E-4
130-134	5.306341357711373E-5
135-139	6.560567496806788E-5
140-144	5.981693894147366E-5
145-149	7.814793635902204E-5
150	1.157747205318845E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.0729914E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.27210602784457
#Duplication Level	Percentage of total
1	32.87419304751404
2	8.615571071428306
3	5.095965774932975
4	3.5606820068129243
5	2.6930808161790134
6	2.1890937212176587
7	1.794881851760173
8	1.5378495103228667
9	1.3209484541988672
>10	16.25232735473076
>50	5.395406946798965
>100	10.48449546270349
>500	3.476525974888362
>1k	4.195338821661087
>5k	0.4477422937114675
>10k+	0.06589689113896756
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	9.647893377657041E-6	0.0	9.647893377657041E-6	1.4471840066485563E-4	9.647893377657041E-6
2	2.8943680132971126E-5	5.788736026594225E-5	0.0	9.647893377657041E-6	0.0016063742473798974	1.4471840066485563E-5
3	3.376762682179965E-5	6.75352536435993E-5	0.0	9.647893377657041E-6	0.0037289107904644467	1.9295786755314083E-5
4	6.75352536435993E-5	6.75352536435993E-5	0.0	1.4471840066485563E-5	0.005923806533881424	1.9295786755314083E-5
5	7.235920033242781E-5	6.75352536435993E-5	0.0	1.9295786755314083E-5	0.006830708511381185	1.9295786755314083E-5
6	1.2059866722071302E-4	6.75352536435993E-5	0.0	1.9295786755314083E-5	0.00844673065213874	1.9295786755314083E-5
7	1.3024656059837007E-4	8.200709371008486E-5	0.0	1.9295786755314083E-5	0.009831203351832525	2.4119733444142606E-5
8	2.5084522781908307E-4	8.200709371008486E-5	0.0	1.9295786755314083E-5	0.012686979791619011	2.4119733444142606E-5
9	3.3285232152916795E-4	8.200709371008486E-5	0.0	1.9295786755314083E-5	0.01513272076285507	2.8943680132971126E-5
10-11	4.0762349520601E-4	8.683104039891338E-5	0.0	1.9295786755314083E-5	0.026338748921003723	6.75352536435993E-5
12-13	4.413911220278097E-4	8.924301374332763E-5	0.0	1.9295786755314083E-5	0.03299338337824267	9.16549870877419E-5
14-15	4.848066422272664E-4	1.0853880049864172E-4	0.0	1.9295786755314083E-5	0.03841308748314151	9.406696043215616E-5
16-17	5.28222162426723E-4	1.1095077384305599E-4	0.0	1.9295786755314083E-5	0.04193456856598633	1.0130288046539894E-4
18-19	6.174651761700506E-4	1.1336274718747025E-4	0.0	1.9295786755314083E-5	0.04508219378044694	1.0130288046539894E-4
20-21	9.35845657632733E-4	1.3265853394278432E-4	0.0	1.9295786755314083E-5	0.047530346725027414	1.0371485380981319E-4
22-23	0.0012807578458839722	1.3748248063161285E-4	0.0	1.9295786755314083E-5	0.049968851776230234	1.157747205318845E-4
24-25	0.001398944539760271	1.398944539760271E-4	0.0	1.9295786755314083E-5	0.05256895904150881	1.2059866722071302E-4
26-27	0.0014520079533373847	1.398944539760271E-4	0.0	1.9295786755314083E-5	0.054800034385092	1.4230642732044138E-4
28-29	0.00150024742022567	1.398944539760271E-4	0.0	1.9295786755314083E-5	0.057240951409639224	1.543662940425127E-4
30-31	0.0016522017409237684	1.398944539760271E-4	0.0	4.5827493543870954E-5	0.06009190390273689	1.8572194751989807E-4
32-33	0.0017438567280115102	1.4471840066485563E-4	0.0	5.065144023269947E-5	0.06283190562199148	2.0984168096404066E-4
34-35	0.0018451596084769093	1.4471840066485563E-4	0.0	7.235920033242781E-5	0.06515945989935125	2.1707760099728344E-4
36-37	0.0018789272352987088	1.4713037400926988E-4	0.0	7.95951203656706E-5	0.06732782393597966	2.2672549437494048E-4
38-39	0.001898223022054023	1.5677826738692694E-4	0.0	9.16549870877419E-5	0.06977356490721573	2.2913746771935475E-4
40-41	0.0019175188088093372	1.6401418742016972E-4	0.0	9.16549870877419E-5	0.07196604867728829	2.31549441063769E-4
42-43	0.00192475472884258	1.6883813410899825E-4	0.0	9.647893377657042E-5	0.07436355018163607	2.387853610970118E-4
44-45	0.0019416385422534796	1.7366208079782675E-4	0.0	9.889090712098469E-5	0.07688647429989338	2.4119733444142604E-4
46-47	0.0019464624889423082	1.7366208079782675E-4	0.0	1.0612682715422746E-4	0.08054061391668099	2.4119733444142604E-4
48-49	0.001963346302353208	1.7366208079782675E-4	0.0	1.1095077384305599E-4	0.08286575622069633	2.4119733444142604E-4
50-51	0.0019826420891085224	1.7848602748665528E-4	0.0	1.1095077384305599E-4	0.08539350428564248	2.4119733444142604E-4
52-53	0.0020164097159303214	1.8089800083106954E-4	0.0	1.1095077384305599E-4	0.08742438584163928	2.4602128113025457E-4
54-55	0.002035705502685636	1.833099741754838E-4	0.0	1.1095077384305599E-4	0.08894392904862027	2.4602128113025457E-4
56-57	0.002112888649706892	1.9054589420872657E-4	0.0	1.1095077384305599E-4	0.09087109575080726	2.5084522781908307E-4
58-59	0.0021490682498731062	1.9295786755314085E-4	0.0	1.157747205318845E-4	0.09272107930597301	2.5084522781908307E-4
60-61	0.002221427450205534	1.9295786755314085E-4	0.0	1.157747205318845E-4	0.09469407350170386	2.5084522781908307E-4
62-63	0.002262430997060576	2.122536543084549E-4	0.0	1.157747205318845E-4	0.096884145298432	2.5084522781908307E-4
64-65	0.002293786650537962	2.122536543084549E-4	0.0	1.157747205318845E-4	0.0994022454700005	2.532572011634973E-4
66-67	0.002305846517260033	2.194895743416977E-4	0.0	1.2059866722071302E-4	0.10159955318676189	2.580811478523259E-4
68-69	0.002351674010803904	2.2913746771935475E-4	0.0	1.2301064056512728E-4	0.10322763519424152	2.6772904122998294E-4
70-71	0.0023830296642812893	2.4119733444142604E-4	0.0	1.2542261390954154E-4	0.10499802362904159	2.797889079520542E-4
72-73	0.002407149397725432	2.4119733444142604E-4	0.0	1.2542261390954154E-4	0.10646209144910104	2.797889079520542E-4
74-75	0.0024288571578251605	2.4119733444142604E-4	0.0	1.3265853394278432E-4	0.10785862401551691	2.8461285464088275E-4
76-77	0.0024409170245472318	2.4360930778584032E-4	0.0	1.3748248063161285E-4	0.10908149450113494	2.8461285464088275E-4
78-79	0.002465036757991374	2.484332544746688E-4	0.0	1.6160221407575544E-4	0.11019582618625431	2.87024827985297E-4
80-81	0.0025349839849793878	2.5084522781908307E-4	0.0	1.6883813410899822E-4	0.11156100309919278	2.9426074801853976E-4
82-83	0.0025759875318344303	2.5084522781908307E-4	0.0	1.6883813410899822E-4	0.1130347188126299	3.0149666805178257E-4
84-85	0.0026338748921003723	2.5566917450791163E-4	0.0	1.6883813410899822E-4	0.11442883940570134	3.039086413961968E-4
86-87	0.002703822119088386	2.6531706788556863E-4	0.0	1.6883813410899822E-4	0.11571200922492973	3.039086413961968E-4
88-89	0.0027182939591548715	2.701410145743972E-4	0.0	1.712501074534125E-4	0.11701206285756902	3.039086413961968E-4
90-91	0.0027400017192546	2.701410145743972E-4	0.0	1.7366208079782675E-4	0.11867873643855928	3.039086413961968E-4
92-93	0.0028027130262093707	2.7255298791881144E-4	0.0	1.7366208079782675E-4	0.12030440647269448	3.039086413961968E-4
94-95	0.0028750722265417986	2.797889079520542E-4	0.0	1.7366208079782675E-4	0.1216623474655997	3.039086413961968E-4
96-97	0.002923311693430084	2.8220088129646845E-4	0.0	1.7366208079782675E-4	0.12290692571131748	3.039086413961968E-4
98-99	0.0029860230003848545	2.8943680132971126E-4	0.0	1.8330997417548381E-4	0.12392960240934912	3.039086413961968E-4
100-101	0.0031114456142943957	3.039086413961968E-4	0.0	1.953698408975551E-4	0.12500534252095788	3.0632061474061107E-4
102-103	0.003150037187805024	3.039086413961968E-4	0.0	2.0260576093079788E-4	0.12606661079250014	3.087325880850253E-4
104-105	0.0031717449479047527	3.039086413961968E-4	0.0	2.1707760099728344E-4	0.12705551986371	3.087325880850253E-4
106-107	0.0032658119083369087	3.039086413961968E-4	0.0	2.2190154768611195E-4	0.1280540768282975	3.087325880850253E-4
108-109	0.0033068154551919512	3.039086413961968E-4	0.0	2.2672549437494048E-4	0.12904298589950736	3.087325880850253E-4
110-111	0.003381586628868793	3.111445614294396E-4	0.0	2.2672549437494048E-4	0.13000777523727305	3.111445614294396E-4
112-113	0.003507009242778335	3.135565347738539E-4	0.0	2.2672549437494048E-4	0.1311293428424257	3.135565347738539E-4
114-115	0.0035576606830110343	3.183804814626824E-4	0.0	2.31549441063769E-4	0.13230397386115544	3.1596850811826813E-4
116-117	0.003627607909999048	3.183804814626824E-4	0.0	2.4119733444142604E-4	0.1332277596520661	3.183804814626824E-4
118-119	0.003704791057020304	3.2079245480709663E-4	0.0	2.4119733444142604E-4	0.1340405946691337	3.232044281515109E-4
120-121	0.003842273537651917	3.232044281515109E-4	0.0	2.4119733444142604E-4	0.13486307757957897	3.232044281515109E-4
122-123	0.004129298365637213	3.232044281515109E-4	0.0	2.4360930778584032E-4	0.13567350062330213	3.232044281515109E-4
124-125	0.0042764287396464835	3.232044281515109E-4	0.0	2.4602128113025457E-4	0.13640191657331524	3.256164014959252E-4
126-127	0.004339140046601254	3.232044281515109E-4	0.0	2.4602128113025457E-4	0.13720510369700523	3.2802837484033944E-4
128-129	0.004440442927066654	3.232044281515109E-4	0.0	2.4602128113025457E-4	0.13796005135380687	3.2802837484033944E-4
130-131	0.0045682775143206095	3.232044281515109E-4	0.0	2.6531706788556863E-4	0.13863540389024287	3.304403481847537E-4
132-133	0.004643048687997451	3.232044281515109E-4	0.0	2.6772904122998294E-4	0.13930593247999	3.352642948735822E-4
134-135	0.004756411435184921	3.232044281515109E-4	0.0	2.701410145743972E-4	0.14019353867073447	3.3767626821799645E-4
136-137	0.00492524956929392	3.376762682179965E-4	4.823946688828521E-6	2.701410145743972E-4	0.14110767656826748	3.3767626821799645E-4
138-139	0.005002432716315176	3.473241615956535E-4	4.823946688828521E-6	2.749649612632257E-4	0.14203146235917813	3.3767626821799645E-4
>>END_MODULE
