##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.control.4d.R.3.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24130301
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.82887755938063	36.0	34.0	38.0	30.0	39.0
2	34.642136415952706	35.0	34.0	37.0	31.0	39.0
3	34.853954577690516	35.0	34.0	38.0	31.0	39.0
4	34.83628210854063	35.0	34.0	38.0	31.0	39.0
5	34.70218291102129	35.0	34.0	37.0	31.0	39.0
6	34.69089813674517	35.0	34.0	37.0	31.0	39.0
7	34.746819776512524	35.0	34.0	37.0	32.0	39.0
8	34.84597415506752	35.0	34.0	37.0	32.0	39.0
9	34.81780343311922	35.0	34.0	37.0	32.0	39.0
10-14	34.80409577153638	35.0	34.0	37.0	31.2	39.0
15-19	34.8224151949037	35.0	34.0	37.0	31.0	39.0
20-24	34.81296281384969	35.0	34.0	37.0	31.0	39.0
25-29	34.80965871913492	35.0	34.0	37.0	31.0	39.0
30-34	34.79589674409781	35.0	34.0	37.0	31.0	39.0
35-39	34.78718027595263	35.0	34.0	37.0	31.0	39.0
40-44	34.74658167753481	35.0	34.0	37.0	31.0	39.0
45-49	34.6499313207904	35.0	34.0	37.0	31.0	39.0
50-54	34.62592077902385	35.0	34.0	37.0	31.0	39.0
55-59	34.611196586399814	35.0	34.0	37.0	31.0	39.0
60-64	34.59108788572509	35.0	34.0	37.0	31.0	39.0
65-69	34.55283376697208	35.0	34.0	37.0	30.8	39.0
70-74	34.55769928439766	35.0	34.0	37.0	30.4	39.0
75-79	34.53951292194822	35.0	34.0	37.0	30.0	39.0
80-84	34.529079309868536	35.0	34.0	37.0	30.0	39.0
85-89	34.50613321400342	35.0	34.0	37.0	30.0	39.0
90-94	34.4867735632473	35.0	34.0	37.0	30.0	39.0
95-99	34.469864739772625	35.0	34.0	37.0	30.0	39.0
100-104	34.43270074418052	35.0	34.0	37.0	30.0	39.0
105-109	34.406914203018026	35.0	34.0	37.0	30.0	39.0
110-114	34.372911046571694	35.0	34.0	37.0	30.0	39.0
115-119	34.33593679581536	35.0	34.0	37.0	30.0	39.0
120-124	34.29616638433147	35.0	34.0	37.0	30.0	39.0
125-129	34.28421631375423	35.0	34.0	37.0	30.0	39.0
130-134	34.231831604587114	35.0	34.0	37.0	30.0	39.0
135-139	34.19616261728355	35.0	34.0	37.0	29.8	39.0
140-144	34.14681861614573	35.0	34.0	37.0	29.0	39.0
145-149	34.11684985612074	35.0	34.0	37.0	29.0	39.0
150	34.110934173593606	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	17.0
22	306.0
23	3054.0
24	20150.0
25	79730.0
26	205349.0
27	374604.0
28	554246.0
29	725451.0
30	910612.0
31	1146797.0
32	1478490.0
33	1956630.0
34	2732806.0
35	4300119.0
36	8629019.0
37	1012911.0
38	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.23819070999688	10.66156723294456	12.049770203480186	41.05047185357837
2	21.916799255906135	11.997175002752765	38.58139038450803	27.504635356833067
3	19.95444019091176	16.021290683900155	28.654664582538025	35.36960454265006
4	25.668266344592688	24.083725696608337	22.783951810907745	27.464056147891235
5	25.988992834356132	29.78702568776551	24.97582601711103	19.248155460767325
6	19.26333555750967	36.062526582264006	26.382850659716727	18.2912872005096
7	15.905461369621676	25.110761232648564	40.44008350668397	18.54369389104579
8	17.938128735842724	24.430829914217686	32.39988868750509	25.231152662434496
9	19.162331897363945	22.619462282917397	33.76953091247662	24.448674907242037
10-14	20.18440370574488	29.04521190134578	27.3656629686504	23.40472142425894
15-19	20.385720423479693	27.78145647308233	27.955122424052103	23.877700679385875
20-24	20.55244721697173	27.934512188252718	27.679713831350984	23.83332676342457
25-29	20.517064451375543	28.15105720950267	27.58147604278154	23.750402296340248
30-34	20.531404792040632	27.82584098694626	27.58921091113325	24.053543309879856
35-39	20.62132522209306	27.84042040345694	27.631951933828102	23.9063024406219
40-44	20.658087476056178	27.955755155416483	27.519005950079357	23.867151418447982
45-49	20.76253251996328	27.715660093236977	27.47320513167561	24.048602255124134
50-54	20.8992158465678	27.687685615353217	27.566330194228684	23.8467683438503
55-59	20.90538247692752	27.748846200037747	27.457292016590284	23.888479306444445
60-64	20.832842214278468	27.64735562921151	27.47552577725853	24.044276379251485
65-69	20.95541774360673	27.61110160576162	27.564537141906413	23.868943508725234
70-74	21.052229724909047	27.584063415282333	27.51824066605965	23.84546619374897
75-79	21.050481494859486	27.456422779887895	27.512541510106725	23.98055421514589
80-84	21.125082769456696	27.480881902811067	27.499281193527896	23.894754134204337
85-89	21.099971176467587	27.4966636114739	27.53118786438445	23.87217734767406
90-94	21.079494938016847	27.36056950221767	27.513874898851093	24.04606066091439
95-99	21.192428310330378	27.281631816243063	27.63975536491796	23.886184508508602
100-104	21.23335259930389	27.399572709539143	27.47549887892739	23.891575812229576
105-109	21.189368127797962	27.205651821208004	27.57540724256147	24.02957280843256
110-114	21.277565991822044	27.204564269264715	27.64254665632757	23.87532308258567
115-119	21.331733916089725	27.242411620038297	27.57682949903194	23.84902496484004
120-124	21.28277036589982	27.149051187013324	27.581599063961004	23.98657938312585
125-129	21.310122643374076	27.09692102022786	27.66869658436758	23.92425975203048
130-134	21.38414055342438	27.24191876963678	27.521087374151232	23.852853302787604
135-139	21.396454177686667	27.078734048191993	27.613303674454837	23.911508099666502
140-144	21.473233909465403	27.05593206944185	27.737930532037968	23.73290348905477
145-149	21.47966933143104	27.57399180049591	27.435990964484365	23.510347903588688
150	21.33584000354741	27.060708151602853	27.56844355547396	24.035008289375778
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.5
9	2.0
10	4.0
11	5.0
12	7.5
13	21.0
14	34.5
15	70.5
16	144.0
17	297.5
18	606.0
19	1120.5
20	2083.5
21	3460.0
22	5304.5
23	8355.0
24	13161.5
25	20448.0
26	30127.0
27	42756.0
28	58538.5
29	82170.0
30	112815.0
31	144753.5
32	175597.0
33	209809.5
34	249403.0
35	297555.5
36	372553.5
37	476440.0
38	599148.0
39	748567.5
40	920356.5
41	1089395.5
42	1262788.0
43	1441457.5
44	1586840.5
45	1676647.5
46	1701340.5
47	1658535.0
48	1568979.0
49	1440625.5
50	1271523.0
51	1088193.0
52	903548.0
53	719395.5
54	554080.0
55	416262.5
56	311048.0
57	232720.0
58	173201.5
59	128568.0
60	91363.0
61	67425.5
62	50047.0
63	32593.5
64	21420.0
65	17641.0
66	19173.5
67	14631.0
68	6693.5
69	3351.5
70	2294.0
71	1441.5
72	752.0
73	323.0
74	117.0
75	78.0
76	41.0
77	30.5
78	13.5
79	1.5
80	0.5
81	1.0
82	1.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.657666848001606E-4
2	4.973000544004818E-5
3	0.1484399220714238
4	1.118925122401084E-4
5	8.28833424000803E-5
6	8.28833424000803E-5
7	7.045084104006824E-5
8	1.4919001632014454E-4
9	1.1603667936011241E-4
10-14	2.0057768860819435E-4
15-19	2.784880304642698E-4
20-24	3.423082041123316E-4
25-29	3.273892024803172E-4
30-34	2.6191136198425375E-4
35-39	1.375863483841333E-4
40-44	2.826321975842738E-4
45-49	1.9146052094418548E-4
50-54	9.697351060809394E-5
55-59	1.4504584920014052E-4
60-64	1.4587468262414131E-4
65-69	0.05825207070562444
70-74	1.7322618561616782E-4
75-79	1.367575149601325E-4
80-84	9.531584376009234E-5
85-89	7.376617473607146E-5
90-94	1.4504584920014052E-4
95-99	1.0443301142410116E-4
100-104	7.210850788806987E-5
105-109	1.077483451201044E-4
110-114	6.464900707206263E-5
115-119	5.055883886404898E-5
120-124	1.0028884430409718E-4
125-129	1.1935201305611561E-4
130-134	3.646867065603533E-5
135-139	4.6414671744044964E-5
140-144	4.973000544004818E-5
145-149	5.138767228804979E-5
150	6.630667392006424E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4130301E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.34596861683261
#Duplication Level	Percentage of total
1	39.35421035026627
2	9.984250081905959
3	6.074508113154584
4	4.317507241148393
5	3.258970192031073
6	2.621383582460881
7	2.219033240515837
8	1.8412059405014658
9	1.5593013456847618
>10	17.977932299338647
>50	4.729258232457996
>100	5.094187802876026
>500	0.4884585171049369
>1k	0.4797930605532147
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	8.28833424000803E-6	2.0720835600020073E-5	0.0	4.144167120004015E-6	9.117167664008833E-5	8.28833424000803E-6
2	8.28833424000803E-6	7.045084104006826E-5	0.0	8.28833424000803E-6	0.0018068568643217506	3.315333696003212E-5
3	8.28833424000803E-6	7.873917528007628E-5	0.0	8.28833424000803E-6	0.0028719078141627823	6.216250680006022E-5
4	2.9009169840028105E-5	9.531584376009234E-5	0.0	1.2432501360012045E-5	0.00437624047872424	7.873917528007628E-5
5	2.9009169840028105E-5	9.946001088009636E-5	0.0	1.2432501360012045E-5	0.0054661564312852955	8.70275095200843E-5
6	5.387417256005219E-5	1.0360417800010037E-4	0.0	1.2432501360012045E-5	0.006531207381126327	8.70275095200843E-5
7	5.387417256005219E-5	1.6576668480016059E-4	0.0	1.2432501360012045E-5	0.007666709172007427	9.117167664008833E-5
8	7.873917528007628E-5	1.740550190401686E-4	0.0	1.2432501360012045E-5	0.009693206893689391	9.946001088009636E-5
9	9.117167664008833E-5	1.740550190401686E-4	0.0	1.2432501360012045E-5	0.012618988880412225	1.0774834512010438E-4
10-11	1.2639709716012247E-4	1.8027126972017466E-4	0.0	1.4504584920014053E-5	0.01705324769881652	1.4919001632014454E-4
12-13	1.5747835056015256E-4	1.8441543684017867E-4	0.0	1.657666848001606E-5	0.020863809365660214	1.6162251768015657E-4
14-15	1.7198293548016663E-4	1.968479382001907E-4	0.0	1.657666848001606E-5	0.025080499410264297	1.8027126972017466E-4
16-17	2.0720835600020074E-4	2.2171294092021478E-4	0.0	1.657666848001606E-5	0.029060971929028154	1.8648752040018068E-4
18-19	2.3414544228022682E-4	2.3621752584022885E-4	0.0	1.657666848001606E-5	0.0327202714959917	1.8648752040018068E-4
20-21	3.3567753672032524E-4	2.83875447720275E-4	0.0	1.657666848001606E-5	0.03591128017839479	1.947758546401887E-4
22-23	4.164887955604035E-4	3.004521162002911E-4	0.0	1.657666848001606E-5	0.03959544474807836	2.1342460668020676E-4
24-25	5.118046393204959E-4	3.149567011203051E-4	0.0	1.657666848001606E-5	0.04370853061468234	2.1549669024020876E-4
26-27	5.946879817205761E-4	3.149567011203051E-4	0.0	1.657666848001606E-5	0.04792522065928643	2.1964085736021278E-4
28-29	6.423459036006223E-4	3.1702878468030713E-4	0.0	1.657666848001606E-5	0.05194920693281033	2.403616929602329E-4
30-31	7.107246610806885E-4	3.2324503536031315E-4	0.0	3.936958764003815E-5	0.055747336098294006	2.5693836144024895E-4
32-33	7.666709172007428E-4	3.2324503536031315E-4	0.0	4.973000544004818E-5	0.05932168023929747	3.108125340003011E-4
34-35	8.28833424000803E-4	3.2324503536031315E-4	0.0	6.216250680006022E-5	0.06251476100526056	3.2946128604031917E-4
36-37	8.37121758240811E-4	3.2324503536031315E-4	0.0	7.459500816007227E-5	0.0654301825741834	3.439658709603332E-4
38-39	8.640588445208371E-4	3.2738920248031716E-4	0.0	8.288334240008029E-5	0.06930083466426715	3.481100380803373E-4
40-41	9.096446828408813E-4	3.2738920248031716E-4	0.0	9.117167664008833E-5	0.07249805959735023	3.543262887603433E-4
42-43	9.137888499608853E-4	3.2946128604031917E-4	0.0	9.946001088009636E-5	0.07562483368939327	3.6468670656035333E-4
44-45	9.552305211609255E-4	3.418937874003312E-4	0.0	1.0153209444009836E-4	0.079478909110997	3.6883087368035734E-4
46-47	0.0010070326101609757	3.439658709603332E-4	0.0	1.0360417800010037E-4	0.08312370409304053	3.7297504080036135E-4
48-49	0.0010443301142410118	3.543262887603433E-4	0.0	1.0360417800010037E-4	0.08684102199968413	3.854075421603734E-4
50-51	0.001069195116961036	3.6468670656035333E-4	0.0	1.0360417800010037E-4	0.09061635824600779	3.916237928403794E-4
52-53	0.0011209972059610862	3.6883087368035734E-4	0.0	1.0360417800010037E-4	0.09433782031977139	3.936958764003814E-4
54-55	0.0011893759634411523	3.7090295724035935E-4	0.0	1.0360417800010037E-4	0.09726153022293423	3.936958764003814E-4
56-57	0.0012225293004011844	3.854075421603734E-4	0.0	1.0360417800010037E-4	0.10067217976269753	4.0612837776039345E-4
58-59	0.0012432501360012044	4.0820046132039546E-4	0.0	1.118925122401084E-4	0.10439778600358114	4.206329626804075E-4
60-61	0.0012971243085612566	4.185608791204055E-4	0.0	1.118925122401084E-4	0.1076551013599043	4.268492133604135E-4
62-63	0.0013302776455212887	4.227050462404095E-4	0.0	1.139645958001104E-4	0.11088133546282741	4.268492133604135E-4
64-65	0.0013551426482413128	4.289212969204155E-4	0.0	1.1810876292011443E-4	0.11500685383079143	4.268492133604135E-4
66-67	0.001369647233161327	4.558583832004416E-4	0.0	1.2018084648011643E-4	0.11864128839503493	4.3099338048041753E-4
68-69	0.0014649630769214194	4.765792188004617E-4	0.0	1.2018084648011643E-4	0.12125211368063746	4.496421325204356E-4
70-71	0.0016100089261215597	4.8486755304046975E-4	0.0	1.2846918072012447E-4	0.12378419979095992	4.641467174404497E-4
72-73	0.001690820184961638	4.869396366004717E-4	0.0	1.3261334784012849E-4	0.12649655717100255	4.724350516804577E-4
74-75	0.001715685187681662	4.973000544004817E-4	0.0	1.409016820801365E-4	0.12942026707416537	4.745071352404597E-4
76-77	0.0017529826917616982	4.973000544004817E-4	0.0	1.4919001632014454E-4	0.13152135980400742	4.8072338592046573E-4
78-79	0.0017737035273617183	5.014442215204859E-4	0.0	1.8441543684017867E-4	0.13382758880628964	4.8072338592046573E-4
80-81	0.0018110010314417546	5.055883886404899E-4	0.0	1.906316875201847E-4	0.13618354781401193	4.8072338592046573E-4
82-83	0.0018607310368818028	5.159488064404999E-4	0.0	1.906316875201847E-4	0.13846698389713413	4.8072338592046573E-4
84-85	0.0019208214601218608	5.180208900005019E-4	4.144167120004015E-6	1.906316875201847E-4	0.14053285120645614	4.8072338592046573E-4
86-87	0.0020202814710019576	5.304533913605139E-4	4.144167120004015E-6	1.906316875201847E-4	0.14239979849401796	4.8072338592046573E-4
88-89	0.002092804395602028	5.34597558480518E-4	4.144167120004015E-6	1.9892002176019271E-4	0.14426052953089974	4.8072338592046573E-4
90-91	0.0021155973147620496	5.42885892720526E-4	4.144167120004015E-6	2.0306418888019673E-4	0.14673666938510216	4.8279546948046774E-4
92-93	0.0021549669024020874	5.42885892720526E-4	4.144167120004015E-6	2.0306418888019673E-4	0.1492666834118646	4.890117201604737E-4
94-95	0.0022357781612421662	5.42885892720526E-4	4.144167120004015E-6	2.0306418888019673E-4	0.1520474195493873	4.890117201604737E-4
96-97	0.0023414544228022686	5.4703005984053E-4	4.144167120004015E-6	2.0306418888019673E-4	0.15427905354350946	4.931558872804777E-4
98-99	0.0024554190186023787	5.61534644760544E-4	4.144167120004015E-6	2.1135252312020475E-4	0.15619365875295133	4.973000544004817E-4
100-101	0.0025693836144024896	5.63606728320546E-4	4.144167120004015E-6	2.1342460668020676E-4	0.15806889437475313	4.973000544004817E-4
102-103	0.0026066811184825255	5.63606728320546E-4	4.144167120004015E-6	2.1964085736021278E-4	0.15963124537899465	4.993721379604838E-4
104-105	0.00265226695680257	5.63606728320546E-4	4.144167120004015E-6	2.258571080402188E-4	0.16118945221611616	5.035163050804879E-4
106-107	0.002739294466322654	5.63606728320546E-4	4.144167120004015E-6	2.3414544228022685E-4	0.16266270362727758	5.076604722004919E-4
108-109	0.0028366823936427483	5.63606728320546E-4	4.144167120004015E-6	2.4036169296023287E-4	0.16406343211383895	5.097325557604939E-4
110-111	0.0029361424045228446	5.6775089544055E-4	4.144167120004015E-6	2.4450586008023685E-4	0.1654310072634403	5.097325557604939E-4
112-113	0.003050107000322955	5.718950625605541E-4	4.144167120004015E-6	2.4450586008023685E-4	0.16706173702516186	5.097325557604939E-4
114-115	0.003143350760523045	5.843275639205661E-4	4.144167120004015E-6	2.548662778802469E-4	0.1687732780457235	5.097325557604939E-4
116-117	0.0032117295180031117	5.843275639205661E-4	4.144167120004015E-6	2.6108252856025296E-4	0.1705117561525652	5.097325557604939E-4
118-119	0.003278036191923176	5.967600652805781E-4	4.144167120004015E-6	2.69370862800261E-4	0.17192284505692657	5.118046393204959E-4
120-121	0.003404433289083298	6.091925666405902E-4	4.144167120004015E-6	2.8594753128027703E-4	0.1731951043627678	5.138767228804979E-4
122-123	0.0035950649766034832	6.133367337605942E-4	4.144167120004015E-6	2.9009169840028105E-4	0.17449222867132905	5.138767228804979E-4
124-125	0.0036965970710435814	6.174809008805982E-4	4.144167120004015E-6	2.9009169840028105E-4	0.17576656006073027	5.180208900005019E-4
126-127	0.0037256062408836093	6.174809008805982E-4	4.144167120004015E-6	2.9009169840028105E-4	0.17707197270353153	5.200929735605039E-4
128-129	0.0037877687476836697	6.174809008805982E-4	4.144167120004015E-6	2.9216378196028305E-4	0.17838152951345282	5.242371406805079E-4
130-131	0.00387065209008375	6.216250680006022E-4	4.144167120004015E-6	2.9423586552028506E-4	0.179601986730294	5.34597558480518E-4
132-133	0.003949391265363826	6.257692351206062E-4	4.144167120004015E-6	2.9630794908028706E-4	0.18078514644305516	5.34597558480518E-4
134-135	0.0041234462844039945	6.257692351206062E-4	4.144167120004015E-6	2.9838003264028907E-4	0.18191028781613622	5.34597558480518E-4
136-137	0.004312005888364177	6.402738200406203E-4	4.144167120004015E-6	2.9838003264028907E-4	0.1830147083536173	5.3666964204052E-4
138-139	0.0044280425677242895	6.796434076806584E-4	4.144167120004015E-6	2.9838003264028907E-4	0.1841481380609384	5.42885892720526E-4
>>END_MODULE
