##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.control.4d.R.3.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24130301
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.579120003517566	37.0	35.0	38.0	32.0	39.0
2	35.15377785797202	35.0	34.0	37.0	32.0	39.0
3	35.33816399555066	35.0	34.0	37.0	33.0	39.0
4	35.37056442022833	36.0	34.0	37.0	33.0	39.0
5	35.29274931133267	35.0	34.0	37.0	33.0	39.0
6	35.24965929766065	35.0	34.0	37.0	33.0	39.0
7	35.29009712725921	35.0	34.0	37.0	33.0	39.0
8	35.41739069065073	35.0	34.0	38.0	33.0	39.0
9	35.388767094119544	35.0	34.0	38.0	33.0	39.0
10-14	35.363931059127694	35.4	34.2	37.4	33.0	39.0
15-19	35.336038684308164	35.0	34.0	37.0	33.0	39.0
20-24	35.302643899883385	35.2	34.0	37.0	32.2	39.0
25-29	35.29251658319554	35.0	34.0	37.0	32.0	39.0
30-34	35.274481607171005	35.0	34.0	37.0	32.0	39.0
35-39	35.24987469489088	35.0	34.0	37.0	32.0	39.0
40-44	35.227912266821704	35.0	34.0	37.0	32.0	39.0
45-49	35.201681935090654	35.0	34.0	37.0	32.0	39.0
50-54	35.176849306604176	35.0	34.0	37.0	32.0	39.0
55-59	35.148058857616405	35.0	34.0	37.0	32.0	39.0
60-64	35.10737059599878	35.0	34.0	37.0	32.0	39.0
65-69	35.07260216936374	35.0	34.0	37.0	32.0	39.0
70-74	35.05252401948902	35.0	34.0	37.0	32.0	39.0
75-79	35.00258156746573	35.0	34.0	37.0	32.0	39.0
80-84	34.928133528048406	35.0	34.0	37.0	31.0	39.0
85-89	34.91179606918289	35.0	34.0	37.0	31.0	39.0
90-94	34.8612155065948	35.0	34.0	37.0	31.0	39.0
95-99	34.79833227940257	35.0	34.0	37.0	31.0	39.0
100-104	34.73428529548803	35.0	34.0	37.0	31.0	39.0
105-109	34.67708673837098	35.0	34.0	37.0	30.8	39.0
110-114	34.61208383600354	35.0	34.0	37.0	30.0	39.0
115-119	34.5549944528251	35.0	34.0	37.0	30.0	39.0
120-124	34.481902592097796	35.0	34.0	37.0	30.0	39.0
125-129	34.40263230035961	35.0	34.0	37.0	30.0	39.0
130-134	34.32628871061327	35.0	34.0	37.0	29.8	39.0
135-139	34.23754898871755	35.0	34.0	37.0	29.0	39.0
140-144	34.13666659193352	35.0	34.0	37.0	29.0	39.0
145-149	34.031713802492554	35.0	34.0	37.0	28.8	39.0
150	33.95991177233968	35.0	34.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	16.0
21	322.0
22	2719.0
23	12244.0
24	39107.0
25	91685.0
26	175690.0
27	293524.0
28	421512.0
29	551258.0
30	700477.0
31	902801.0
32	1193444.0
33	1639449.0
34	2425833.0
35	4071667.0
36	1.0107676E7
37	1500869.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.74508812571746	19.35203457893188	13.155261227585235	34.74761606776543
2	23.91480006464901	26.534734338155765	33.75990766795274	15.790557929242489
3	18.6229463113618	30.353218552889167	29.576696950444177	21.44713818530486
4	22.3187601348197	34.90482775163062	22.77183778188262	20.00457433166706
5	24.40343367453228	35.51841727958553	22.981702549006744	17.09644649687544
6	18.36111783271995	38.76185382022379	23.796682022325374	19.080346324730886
7	19.587625533556334	20.646509962722803	38.6624559718505	21.103408531870365
8	20.808365416095118	23.24656966552426	29.154059419070627	26.791005499309996
9	22.972013486280176	23.16441058899348	29.47824397217424	24.385331952552107
10-14	22.916238349720352	29.10553546937579	25.84895689198335	22.129269288920504
15-19	22.731743793830006	28.328005523014404	27.27174186513463	21.66850881802096
20-24	22.8548308210596	28.32269654921881	27.190513540397404	21.63195908932418
25-29	22.930992033626104	28.36563373163062	27.11893399091872	21.58444024382456
30-34	23.00877639280173	28.105175314638636	27.234094593349667	21.65195369920997
35-39	23.078382652582743	28.096084669644195	27.235923828716434	21.589608849056628
40-44	23.263728869358076	27.80761499825468	27.36877753824952	21.559878594137718
45-49	23.275031670761173	27.656492971223194	27.40066441773768	21.667810940277953
50-54	23.374363406195087	27.60945856091442	27.366177714618463	21.650000318272035
55-59	23.530356293524893	27.5076535514414	27.25449964341514	21.707490511618566
60-64	23.460939836597976	27.468992616378884	27.30395198965815	21.76611555736499
65-69	23.56697001003013	27.37694569164305	27.255183430989945	21.800900867336882
70-74	23.618417358324706	27.35235337511952	27.15492028052199	21.874308986033782
75-79	23.560282153131865	27.395209036140912	27.21866171499477	21.825847095732456
80-84	23.671604054559985	27.43652998495075	27.099422916327608	21.79244304416165
85-89	23.722113101235053	27.351406471911087	27.130943408139725	21.79553701871414
90-94	23.641623865363304	27.28509437159528	27.203528045505937	21.869753717535474
95-99	23.74542364805147	27.424006024624394	27.135675597250113	21.694894730074026
100-104	23.91384011330816	27.410582238489273	26.938512702348806	21.737064945853763
105-109	23.693525414374232	27.54419267293848	27.03756161185059	21.724720300836694
110-114	23.732911578682753	27.56468143517978	26.97555741223452	21.726849573902953
115-119	23.815874489091538	27.5441313392651	26.98033066392334	21.659663507720023
120-124	23.778704625358795	27.462689338189357	27.163727464485422	21.594878571966426
125-129	23.769410916175477	27.52373623520071	27.120606576768353	21.586246271855455
130-134	23.833990301239922	27.44323247356094	27.192622255312937	21.530154969886205
135-139	23.749304246142643	27.468086701446452	27.29431017043675	21.48829888197416
140-144	23.785115849032433	27.598648915309003	27.252346560056147	21.363888675602418
145-149	23.941201898893294	28.06423781831013	26.776175192084228	21.21838509071235
150	23.551757601366017	28.027889084350832	26.926448203029047	21.4939051112541
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	8.0
3	27.5
4	35.0
5	27.5
6	23.0
7	20.5
8	14.0
9	9.5
10	7.0
11	7.0
12	9.0
13	10.5
14	21.5
15	40.0
16	74.5
17	151.5
18	256.0
19	427.5
20	644.5
21	1009.0
22	1563.5
23	2565.0
24	4359.5
25	7219.5
26	11482.5
27	18237.5
28	29932.5
29	46062.0
30	61577.0
31	80880.0
32	107358.0
33	140637.0
34	189435.5
35	261150.5
36	363173.0
37	489673.0
38	639097.0
39	824928.5
40	1035878.5
41	1229902.0
42	1411379.5
43	1582678.0
44	1697914.5
45	1745950.0
46	1744470.0
47	1687477.5
48	1574408.0
49	1425483.0
50	1240218.0
51	1037252.0
52	843346.0
53	668530.0
54	518870.5
55	390824.0
56	290426.0
57	215310.5
58	155919.5
59	111352.0
60	75923.5
61	50044.0
62	33977.0
63	23836.0
64	16570.0
65	11224.5
66	8105.0
67	6289.5
68	5629.5
69	4003.0
70	1840.0
71	1106.5
72	608.0
73	476.5
74	407.0
75	263.0
76	171.0
77	62.0
78	17.0
79	2.0
80	0.5
81	0.5
82	0.0
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.144167120004015E-6
2	4.144167120004015E-6
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	4.144167120004015E-6
9	0.0
10-14	1.657666848001606E-6
15-19	0.0
20-24	0.03044222283012549
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.28833424000803E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.059192796641865345
85-89	1.657666848001606E-6
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	8.28833424000803E-7
145-149	0.03139786776799842
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4130301E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.82033246935061
#Duplication Level	Percentage of total
1	28.849283533839525
2	10.18598216709564
3	6.876361296593501
4	5.019409710095796
5	3.741870116486635
6	2.9502808047690894
7	2.5285212825833274
8	2.097254590058214
9	1.7897010704310088
>10	20.895923317645316
>50	5.455663883755516
>100	6.898087327003524
>500	1.377460088963036
>1k	1.1640652429419989
>5k	0.1202028350063297
>10k+	0.04993273273183823
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.2432501360012045E-5	0.0	0.0	0.0012556826373612165	0.0
2	1.2432501360012045E-5	3.315333696003212E-5	0.0	0.0	0.006419314868886219	0.0
3	2.9009169840028105E-5	3.315333696003212E-5	0.0	0.0	0.008342208412568082	0.0
4	8.70275095200843E-5	3.315333696003212E-5	0.0	0.0	0.010074470268729761	0.0
5	8.70275095200843E-5	3.315333696003212E-5	0.0	0.0	0.011947633806971576	0.0
6	9.946001088009636E-5	3.315333696003212E-5	4.144167120004015E-6	4.144167120004015E-6	0.013804220676733373	0.0
7	1.0774834512010438E-4	4.1441671200040147E-5	4.144167120004015E-6	4.144167120004015E-6	0.015942610910655446	0.0
8	1.8234335328017666E-4	4.1441671200040147E-5	4.144167120004015E-6	8.28833424000803E-6	0.022158861590661466	0.0
9	1.947758546401887E-4	4.5585838320044166E-5	4.144167120004015E-6	8.28833424000803E-6	0.02827150809266739	0.0
10-11	2.2171294092021478E-4	5.1802089000050185E-5	6.216250680006022E-6	8.28833424000803E-6	0.035094879255754	0.0
12-13	2.5693836144024895E-4	6.009042324005821E-5	8.28833424000803E-6	8.28833424000803E-6	0.041762844151840464	0.0
14-15	2.9423586552028506E-4	6.630667392006424E-5	8.28833424000803E-6	8.28833424000803E-6	0.05086757931448928	0.0
16-17	3.1702878468030713E-4	6.630667392006424E-5	8.28833424000803E-6	8.28833424000803E-6	0.05653472785109477	0.0
18-19	3.936958764003814E-4	7.045084104006826E-5	8.28833424000803E-6	8.28833424000803E-6	0.06435684329010236	0.0
20-21	4.6829088456045364E-4	7.459500816007227E-5	8.28833424000803E-6	8.28833424000803E-6	0.07078859066034858	0.0
22-23	5.283813078005119E-4	8.70275095200843E-5	8.28833424000803E-6	8.28833424000803E-6	0.07766583599599525	0.0
24-25	6.071204830805882E-4	9.117167664008833E-5	8.28833424000803E-6	8.28833424000803E-6	0.0831920828505206	0.0
26-27	6.609946556406403E-4	9.531584376009234E-5	8.28833424000803E-6	8.28833424000803E-6	0.087727873763365	0.0
28-29	7.625267500807388E-4	9.531584376009234E-5	8.28833424000803E-6	8.28833424000803E-6	0.09264907221836977	0.0
30-31	9.552305211609255E-4	9.946001088009636E-5	8.28833424000803E-6	8.28833424000803E-6	0.09773189319105469	0.0
32-33	0.0011769434620811402	1.118925122401084E-4	8.28833424000803E-6	8.28833424000803E-6	0.1028105699966196	0.0
34-35	0.0013344218126412927	1.1603667936011242E-4	8.28833424000803E-6	8.28833424000803E-6	0.10633932829930302	0.0
36-37	0.0013717193167213288	1.1603667936011242E-4	8.28833424000803E-6	8.28833424000803E-6	0.11017268288530674	0.0
38-39	0.0014214493221613772	1.2225293004011843E-4	8.28833424000803E-6	8.28833424000803E-6	0.11372630619071018	0.0
40-41	0.0014628909933614173	1.346854314001305E-4	8.28833424000803E-6	8.28833424000803E-6	0.11680127819375316	4.144167120004015E-6
42-43	0.0015022605810014553	1.388295985201345E-4	8.28833424000803E-6	8.28833424000803E-6	0.11965660933943592	4.144167120004015E-6
44-45	0.0015478464193214997	1.5333418344014855E-4	8.28833424000803E-6	8.28833424000803E-6	0.12215139794567834	4.144167120004015E-6
46-47	0.0015830718398415337	2.1549669024020876E-4	8.28833424000803E-6	8.28833424000803E-6	0.12496528742016107	4.144167120004015E-6
48-49	0.0016286576781615778	2.1964085736021278E-4	1.2432501360012045E-5	8.28833424000803E-6	0.127992601501324	4.144167120004015E-6
50-51	0.00167217143292162	2.279291916002208E-4	1.657666848001606E-5	8.28833424000803E-6	0.1303775696788863	4.144167120004015E-6
52-53	0.0017239735219216701	2.3828960940023086E-4	1.657666848001606E-5	8.28833424000803E-6	0.13273145660304858	4.144167120004015E-6
54-55	0.0018192893656817626	2.5693836144024895E-4	1.657666848001606E-5	8.28833424000803E-6	0.13492164892597072	4.144167120004015E-6
56-57	0.001914605209441855	2.67298779240259E-4	1.657666848001606E-5	1.2432501360012045E-5	0.1371574270872129	4.144167120004015E-6
58-59	0.0020078489696419453	2.81803364160273E-4	1.657666848001606E-5	1.2432501360012045E-5	0.1392212223129749	4.144167120004015E-6
60-61	0.0021073089805220416	2.9009169840028105E-4	1.657666848001606E-5	1.4504584920014053E-5	0.14075249206381635	4.144167120004015E-6
62-63	0.0021611831530820936	2.9838003264028907E-4	1.657666848001606E-5	1.657666848001606E-5	0.14278935020329833	4.144167120004015E-6
64-65	0.0021839760722421157	3.149567011203051E-4	1.657666848001606E-5	1.657666848001606E-5	0.14456719789778005	4.144167120004015E-6
66-67	0.002300012751602228	3.605425394403493E-4	1.657666848001606E-5	1.657666848001606E-5	0.14608810723082152	4.144167120004015E-6
68-69	0.0023310940050022583	3.7711920792036537E-4	1.657666848001606E-5	1.657666848001606E-5	0.14737279903802278	4.144167120004015E-6
70-71	0.002370463592642296	3.833354586003714E-4	1.657666848001606E-5	1.657666848001606E-5	0.14879217627662417	4.144167120004015E-6
72-73	0.0024015448460423267	3.9991212708038743E-4	1.657666848001606E-5	1.657666848001606E-5	0.14972461387862507	4.144167120004015E-6
74-75	0.002426409848762351	4.0820046132039546E-4	1.657666848001606E-5	1.8648752040018067E-5	0.15094714317902624	4.144167120004015E-6
76-77	0.0024554190186023787	4.268492133604135E-4	1.657666848001606E-5	2.0720835600020073E-5	0.15229814166014755	4.144167120004015E-6
78-79	0.002523797776082445	4.5585838320044166E-4	1.657666848001606E-5	2.0720835600020073E-5	0.15408013352174926	4.144167120004015E-6
80-81	0.002538302361002459	4.724350516804577E-4	1.657666848001606E-5	2.0720835600020073E-5	0.1555678895178307	4.144167120004015E-6
82-83	0.002554879029482475	4.993721379604838E-4	1.657666848001606E-5	2.0720835600020073E-5	0.15717789844395227	4.144167120004015E-6
84-85	0.0025755998650824953	5.055883886404899E-4	1.657666848001606E-5	2.0720835600020073E-5	0.15890601613299393	8.28833424000803E-6
86-87	0.0026170415362825354	5.221650571205059E-4	2.0720835600020073E-5	2.0720835600020073E-5	0.16076674716987577	8.28833424000803E-6
88-89	0.002631546121202549	5.34597558480518E-4	2.0720835600020073E-5	2.0720835600020073E-5	0.16265648737659757	8.28833424000803E-6
90-91	0.00265226695680257	5.63606728320546E-4	2.0720835600020073E-5	2.0720835600020073E-5	0.1636386549840385	8.28833424000803E-6
92-93	0.0026564111239225736	5.63606728320546E-4	2.0720835600020073E-5	2.0720835600020073E-5	0.1646394713435195	8.28833424000803E-6
94-95	0.0026646994581625816	5.6775089544055E-4	2.0720835600020073E-5	2.0720835600020073E-5	0.16559055769756043	1.2432501360012045E-5
96-97	0.0026646994581625816	5.905438146005721E-4	2.0720835600020073E-5	2.0720835600020073E-5	0.16656236488720136	1.657666848001606E-5
98-99	0.0026709157088425873	6.071204830805882E-4	2.0720835600020073E-5	2.0720835600020073E-5	0.16748651415496224	1.657666848001606E-5
100-101	0.0026729877924025897	6.154088173205962E-4	2.0720835600020073E-5	2.0720835600020073E-5	0.16830084299404305	2.0720835600020073E-5
102-103	0.0026729877924025897	6.216250680006022E-4	2.0720835600020073E-5	2.0720835600020073E-5	0.16913174850160384	2.0720835600020073E-5
104-105	0.0026771319595225935	6.257692351206062E-4	2.0720835600020073E-5	2.0720835600020073E-5	0.17004346526800473	2.2792919160022083E-5
106-107	0.0027061411293626218	6.382017364806183E-4	2.486500272002409E-5	2.0720835600020073E-5	0.17082256868656548	2.486500272002409E-5
108-109	0.002718573630722634	6.382017364806183E-4	2.486500272002409E-5	2.0720835600020073E-5	0.1715540141832462	2.486500272002409E-5
110-111	0.002731006132082646	6.423459036006223E-4	2.486500272002409E-5	2.0720835600020073E-5	0.17233311760180695	2.486500272002409E-5
112-113	0.0027413665498826558	6.444179871606243E-4	2.486500272002409E-5	2.486500272002409E-5	0.1730790676834077	2.9009169840028105E-5
114-115	0.0027434386334426577	6.506342378406303E-4	2.486500272002409E-5	2.486500272002409E-5	0.17399907278404858	3.1081253400030115E-5
116-117	0.0027496548841226638	6.589225720806383E-4	2.486500272002409E-5	2.9009169840028105E-5	0.17490457329976944	3.7297504080036134E-5
118-119	0.00275587113480267	6.609946556406403E-4	2.9009169840028105E-5	3.1081253400030115E-5	0.1756691721334102	4.1441671200040147E-5
120-121	0.00275587113480267	6.713550734406504E-4	2.9009169840028105E-5	3.315333696003212E-5	0.17646692430401095	4.1441671200040147E-5
122-123	0.0027600153019226737	6.713550734406504E-4	2.9009169840028105E-5	3.315333696003212E-5	0.1772356673047717	4.1441671200040147E-5
124-125	0.002772447803282686	6.734271570006524E-4	2.9009169840028105E-5	3.315333696003212E-5	0.17809558198217254	4.765792188004617E-5
126-127	0.002789024471762702	6.837875748006624E-4	3.1081253400030115E-5	3.315333696003212E-5	0.17913369584573355	4.973000544004818E-5
128-129	0.0028076732238027204	6.920759090406704E-4	3.315333696003212E-5	3.315333696003212E-5	0.18014487262301454	5.1802089000050185E-5
130-131	0.0028304661429627426	7.086525775206866E-4	3.315333696003212E-5	3.315333696003212E-5	0.18100893146753538	5.59462561200542E-5
132-133	0.002855331145682766	7.086525775206866E-4	3.522542052003413E-5	3.315333696003212E-5	0.18181497197237614	7.873917528007628E-5
134-135	0.0028801961484027903	7.086525775206866E-4	3.7297504080036134E-5	3.7297504080036134E-5	0.18264587747993694	8.70275095200843E-5
136-137	0.0028843403155227945	7.190129953206966E-4	4.1441671200040147E-5	3.7297504080036134E-5	0.18341047631357768	9.117167664008833E-5
138-139	0.002913349485362823	7.231571624407006E-4	4.1441671200040147E-5	3.7297504080036134E-5	0.18410669638973837	9.324376020009034E-5
>>END_MODULE
