##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.2d.R.1.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23706065
Total Bases	3.5 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.706761413165786	37.0	35.0	38.0	33.0	39.0
2	35.32124800130262	35.0	34.0	37.0	33.0	39.0
3	35.4607199465622	35.0	34.0	37.0	33.0	39.0
4	35.51510421489184	36.0	35.0	38.0	33.0	39.0
5	35.42830305240452	36.0	35.0	37.0	33.0	39.0
6	35.38804331296653	35.0	35.0	37.0	33.0	39.0
7	35.41467126661468	35.0	34.0	37.0	33.0	39.0
8	35.506863707662994	36.0	35.0	38.0	33.0	39.0
9	35.508936679284396	36.0	35.0	38.0	33.0	39.0
10-14	35.49138090189156	35.8	35.0	37.8	33.0	39.0
15-19	35.46504530380728	36.0	35.0	38.0	33.0	39.0
20-24	35.4513314461932	36.0	34.8	38.0	33.0	39.0
25-29	35.432835689938415	36.0	34.4	38.0	33.0	39.0
30-34	35.41453640661155	36.0	34.2	38.0	33.0	39.0
35-39	35.38893564157526	36.0	34.0	38.0	33.0	39.0
40-44	35.37215038430038	36.0	34.0	38.0	33.0	39.0
45-49	35.344796025827144	36.0	34.0	38.0	32.6	39.0
50-54	35.324508711167375	36.0	34.0	38.0	32.0	39.0
55-59	35.30041881687239	36.0	34.0	38.0	32.0	39.0
60-64	35.260465834376134	36.0	34.0	38.0	32.0	39.0
65-69	35.23640902865996	36.0	34.0	38.0	32.0	39.0
70-74	35.21034358085157	36.0	34.0	38.0	32.0	39.0
75-79	35.16446408123828	36.0	34.0	38.0	32.0	39.0
80-84	35.12831833541332	36.0	34.0	38.0	32.0	39.0
85-89	35.081051182471654	35.6	34.0	37.8	32.0	39.0
90-94	35.03054061481734	35.0	34.0	37.0	32.0	39.0
95-99	34.97035043985579	35.0	34.0	37.0	31.0	39.0
100-104	34.92577890088465	35.0	34.0	37.0	31.0	39.0
105-109	34.8679387068246	35.0	34.0	37.0	31.0	39.0
110-114	34.79998217333834	35.0	34.0	37.0	31.0	39.0
115-119	34.742572122366155	35.0	34.0	37.0	31.0	39.0
120-124	34.67491788282872	35.0	34.0	37.0	30.2	39.0
125-129	34.60219209725444	35.0	34.0	37.0	30.0	39.0
130-134	34.50595647991348	35.0	34.0	37.0	30.0	39.0
135-139	34.453555189357665	35.0	34.0	37.0	30.0	39.0
140-144	34.330069608768895	35.0	34.0	37.0	29.4	39.0
145-149	34.25918892064119	35.0	34.0	37.0	29.0	39.0
150	34.19514023099152	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	26.0
21	322.0
22	2262.0
23	11089.0
24	34919.0
25	81377.0
26	154349.0
27	259411.0
28	375377.0
29	487933.0
30	618825.0
31	801751.0
32	1063311.0
33	1475665.0
34	2208761.0
35	3792633.0
36	1.0508422E7
37	1829623.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.91841286185624	18.805925825310947	16.352595844143682	30.92306546868913
2	24.28353250528926	25.467630330044233	32.928670363470275	17.320166801196237
3	20.20540313206768	29.3829912303033	30.02046522693665	20.39114041069237
4	23.747901644579137	33.902433828642586	22.312112955060233	20.03755157171804
5	25.28890391551698	35.11747732067722	22.462989956367707	17.130628807438097
6	19.015813041936735	39.162400001856064	22.659374299361787	19.162412656845408
7	20.373976026810016	20.718647316625514	38.108344847615996	20.79903180894847
8	21.657579377177704	23.41563129228263	28.18684575939559	26.739943571144074
9	23.595206543135692	23.266029178608935	28.611408093245338	24.52735618501004
10-14	23.125347880384197	29.27714489941709	25.62340312489652	21.974104095302195
15-19	22.949259609302512	28.3173230141738	27.142334250749755	21.59108312577393
20-24	23.050292825907633	28.358275403361965	27.054999638278222	21.53643213245218
25-29	23.13936960857907	28.273410201144728	26.99853982514601	21.588680365130188
30-34	23.123308286268653	28.03192310544872	27.237585440478384	21.60718316780424
35-39	23.212809886415144	28.00891839282479	27.20063409933281	21.577637621427257
40-44	23.349411216074873	27.76739370283512	27.325763259317814	21.55743182177219
45-49	23.343062629753188	27.613987390990445	27.40359650578871	21.639353473467654
50-54	23.405097387525093	27.578024442268255	27.368462880701628	21.648415289505028
55-59	23.590057649803963	27.4620406212503	27.26494422418904	21.682957504756693
60-64	23.493108620093633	27.43893261070532	27.326554617984893	21.741404151216155
65-69	23.597604241783696	27.375323572258832	27.26438487365997	21.76268731229751
70-74	23.67783096857281	27.326569803972106	27.174216387240985	21.821382840214095
75-79	23.594211017307174	27.410649553183962	27.23527502350137	21.75986440600749
80-84	23.713093674551217	27.437111135905518	27.074774324629587	21.77502086491368
85-89	23.74002011721473	27.38182486211862	27.13405451305394	21.744100507612714
90-94	23.679349567294278	27.320917242064425	27.191261814223488	21.808471376417806
95-99	23.738788364918427	27.419847199440312	27.200203829695056	21.6411606059462
100-104	23.92714269533978	27.39270477829197	27.004177201066476	21.675975325301774
105-109	23.710800590481803	27.535182241337818	27.111790168465326	21.642226999715053
110-114	23.75441194719666	27.543928597484857	27.019049931464924	21.682609523853564
115-119	23.815691891505402	27.52202358341631	27.063943341081703	21.598341183996585
120-124	23.78280579252609	27.449653074012915	27.22094788823029	21.546593245230703
125-129	23.761988293273923	27.541411112857784	27.177052021988533	21.519548571879767
130-134	23.823638897156524	27.439347513418934	27.248630624035957	21.488382965388585
135-139	23.723497763125177	27.45823568778707	27.337413442509334	21.480853106578422
140-144	23.76617376186221	27.591922995233496	27.29270673981532	21.349196503088976
145-149	23.918246237829855	28.280479278193155	26.61227411634955	21.18900036762744
150	23.519403156955825	28.13880751613564	26.777459692277063	21.564329634631473
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	1.5
9	0.5
10	1.0
11	2.5
12	9.5
13	17.5
14	18.0
15	29.0
16	61.5
17	120.0
18	191.0
19	363.5
20	598.5
21	868.5
22	1359.5
23	2344.5
24	4052.0
25	6760.5
26	10561.0
27	16626.0
28	27046.0
29	40530.5
30	55744.5
31	75204.5
32	101504.5
33	135851.0
34	182330.5
35	251866.5
36	351194.0
37	470241.5
38	611207.5
39	792790.5
40	1005343.5
41	1204946.5
42	1382666.0
43	1545670.5
44	1661794.5
45	1711930.5
46	1708186.5
47	1657185.5
48	1555635.5
49	1410157.0
50	1229736.0
51	1032523.0
52	840918.0
53	670273.0
54	523053.5
55	394738.5
56	292116.5
57	215361.0
58	157360.5
59	113877.0
60	79499.5
61	53504.5
62	36403.0
63	25171.5
64	17154.5
65	11504.0
66	8210.0
67	6251.5
68	5855.5
69	4320.0
70	1935.5
71	1186.0
72	687.5
73	481.0
74	404.0
75	263.5
76	177.5
77	67.5
78	13.0
79	1.5
80	0.5
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.11707552476549778
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	1.6873319127404737E-6
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.01951146257297447
115-119	0.0
120-124	0.0
125-129	3.3746638254809474E-6
130-134	0.047487425686211525
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.3706065E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.04783464117685
#Duplication Level	Percentage of total
1	29.92968535895188
2	10.529195520651628
3	6.83880538295127
4	5.046809515402218
5	3.8204645564346227
6	3.026087775784956
7	2.4011949479935892
8	2.0271179791333576
9	1.7918382637012942
>10	20.58929015985816
>50	5.262888783771998
>100	6.395360369284213
>500	1.1983556791382313
>1k	1.0009056139569827
>5k	0.053246749655555405
>10k+	0.08875334333011382
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.2183297818511845E-6	8.436659563702369E-6	0.0	0.0	8.352292968065345E-4	0.0
2	2.5309978691107107E-5	2.109164890925592E-5	0.0	0.0	0.005239165589059171	0.0
3	6.327494672776777E-5	2.5309978691107107E-5	0.0	0.0	0.00690962418267224	0.0
4	1.1811323389183316E-4	2.5309978691107107E-5	4.2183297818511845E-6	0.0	0.008175123117227595	4.2183297818511845E-6
5	1.2233156367368435E-4	2.5309978691107107E-5	4.2183297818511845E-6	0.0	0.00979496175345845	4.2183297818511845E-6
6	1.645148614921962E-4	2.5309978691107107E-5	4.2183297818511845E-6	4.2183297818511845E-6	0.011347307113179687	4.2183297818511845E-6
7	1.645148614921962E-4	2.952830847295829E-5	4.2183297818511845E-6	4.2183297818511845E-6	0.013448035344541576	4.2183297818511845E-6
8	1.898248401833033E-4	3.3746638254809476E-5	4.2183297818511845E-6	4.2183297818511845E-6	0.018927645731166266	4.2183297818511845E-6
9	2.0247982952885686E-4	3.3746638254809476E-5	4.2183297818511845E-6	4.2183297818511845E-6	0.02471097586208424	4.2183297818511845E-6
10-11	2.214623135471872E-4	3.3746638254809476E-5	4.2183297818511845E-6	4.2183297818511845E-6	0.03112705546027989	4.2183297818511845E-6
12-13	2.530997869110711E-4	3.585580314573507E-5	4.2183297818511845E-6	4.2183297818511845E-6	0.037165594542999864	4.2183297818511845E-6
14-15	2.973922496205085E-4	3.796496803666066E-5	4.2183297818511845E-6	4.2183297818511845E-6	0.04494419466073345	4.2183297818511845E-6
16-17	3.227022283116156E-4	3.796496803666066E-5	4.2183297818511845E-6	4.2183297818511845E-6	0.05053769995146812	4.2183297818511845E-6
18-19	3.859771750393834E-4	3.796496803666066E-5	4.2183297818511845E-6	4.2183297818511845E-6	0.05858627317524018	6.327494672776777E-6
20-21	4.429246270943744E-4	4.007413292758625E-5	4.2183297818511845E-6	4.2183297818511845E-6	0.06432952917323057	8.436659563702369E-6
22-23	4.893262546947374E-4	7.171160629147013E-5	8.436659563702369E-6	4.2183297818511845E-6	0.07003060187340245	8.436659563702369E-6
24-25	5.315095525132492E-4	7.171160629147013E-5	8.436659563702369E-6	4.2183297818511845E-6	0.07546170146753584	8.436659563702369E-6
26-27	5.736928503317611E-4	7.382077118239573E-5	8.436659563702369E-6	8.436659563702369E-6	0.07959566465375	8.436659563702369E-6
28-29	6.770419299871151E-4	8.01482658551725E-5	8.436659563702369E-6	1.2654989345553554E-5	0.08351660218598067	8.436659563702369E-6
30-31	8.162468127882042E-4	9.069409030980046E-5	1.2654989345553554E-5	1.2654989345553554E-5	0.08851954130725619	8.436659563702369E-6
32-33	9.174867275526326E-4	9.280325520072606E-5	1.2654989345553554E-5	1.2654989345553554E-5	0.09364903032198722	1.2654989345553554E-5
34-35	0.0010123991476442841	1.0123991476442843E-4	1.2654989345553554E-5	1.2654989345553554E-5	0.09679168600946636	1.2654989345553554E-5
36-37	0.0010883290837176056	1.0545824454627961E-4	1.2654989345553554E-5	1.4764154236479145E-5	0.10024649810080247	1.2654989345553554E-5
38-39	0.0011347307113179685	1.0545824454627961E-4	1.4764154236479145E-5	1.8982484018330328E-5	0.10343133708610011	1.4764154236479145E-5
40-41	0.0011916781633729595	1.075674094372052E-4	1.6873319127404738E-5	2.109164890925592E-5	0.10621121641234005	1.6873319127404738E-5
42-43	0.0012169881420640667	1.1811323389183316E-4	1.6873319127404738E-5	2.109164890925592E-5	0.10899742323325276	1.6873319127404738E-5
44-45	0.001252843945209802	1.349865530192379E-4	1.6873319127404738E-5	2.109164890925592E-5	0.11127110298567056	1.6873319127404738E-5
46-47	0.0013372105408468254	1.624056966012706E-4	1.6873319127404738E-5	2.109164890925592E-5	0.11385904830683624	1.6873319127404738E-5
48-49	0.0013941579929018164	1.666240263831218E-4	2.109164890925592E-5	2.109164890925592E-5	0.11617069302729069	1.6873319127404738E-5
50-51	0.0014447779502840307	1.6873319127404737E-4	2.109164890925592E-5	2.109164890925592E-5	0.11819760048747019	1.6873319127404738E-5
52-53	0.001495397907666245	1.7295152105589856E-4	2.109164890925592E-5	2.109164890925592E-5	0.12038902280914188	1.8982484018330328E-5
54-55	0.0015628911841758639	1.7716985083774975E-4	2.3200813800181514E-5	2.5309978691107107E-5	0.12234210949813898	2.3200813800181514E-5
56-57	0.0016198386362308549	1.8138818061960095E-4	2.5309978691107107E-5	2.5309978691107107E-5	0.12432894282539089	2.5309978691107107E-5
58-59	0.001666240263831218	1.961523348560801E-4	2.5309978691107107E-5	2.5309978691107107E-5	0.12613227880713226	2.5309978691107107E-5
60-61	0.0017421701999045392	2.130256539834848E-4	2.5309978691107107E-5	2.5309978691107107E-5	0.12747159851287002	3.163747336388388E-5
62-63	0.0017864626626139767	2.193531486562616E-4	2.5309978691107107E-5	2.5309978691107107E-5	0.12937406524448491	3.3746638254809476E-5
64-65	0.0017970084870686045	2.3411730289274072E-4	2.5309978691107107E-5	2.5309978691107107E-5	0.13106772465189814	3.3746638254809476E-5
66-67	0.0018687200933600748	2.63645611365699E-4	2.5309978691107107E-5	2.952830847295829E-5	0.13231424110243517	3.3746638254809476E-5
68-69	0.0018855934124874794	2.699731060384758E-4	2.5309978691107107E-5	2.952830847295829E-5	0.13369363494110054	3.7964968036660656E-5
70-71	0.0019151217209604377	2.699731060384758E-4	2.5309978691107107E-5	2.952830847295829E-5	0.135011862997929	4.640162760036303E-5
72-73	0.0019383225347606192	2.699731060384758E-4	2.5309978691107107E-5	2.952830847295829E-5	0.13602004381579144	4.640162760036303E-5
74-75	0.0019636325134517263	2.84737260274955E-4	2.952830847295829E-5	3.796496803666066E-5	0.13713579204309106	4.640162760036303E-5
76-77	0.0019847241623609825	3.1215640385698763E-4	2.952830847295829E-5	3.796496803666066E-5	0.138498312562629	4.640162760036303E-5
78-79	0.0020437807793068988	3.353572176571692E-4	2.952830847295829E-5	3.796496803666066E-5	0.1401202603637508	4.8510792491288625E-5
80-81	0.002064872428216155	3.4168471232994593E-4	2.952830847295829E-5	3.796496803666066E-5	0.1415798024682713	5.48382871640654E-5
82-83	0.0020986190664709645	3.4168471232994593E-4	3.163747336388388E-5	3.796496803666066E-5	0.1430709820461557	5.48382871640654E-5
84-85	0.002126038210052997	3.459030421117971E-4	3.3746638254809476E-5	3.796496803666066E-5	0.14453263331556712	5.48382871640654E-5
86-87	0.002151348188744104	3.6488552613012743E-4	3.796496803666066E-5	3.796496803666066E-5	0.14634862428665407	5.48382871640654E-5
88-89	0.002168221507871509	3.838680101484578E-4	3.796496803666066E-5	4.007413292758625E-5	0.1480380653642855	5.48382871640654E-5
90-91	0.002193531486562616	4.1972381329419284E-4	3.796496803666066E-5	4.218329781851184E-5	0.1489808620705292	5.48382871640654E-5
92-93	0.0022019681461263184	4.2183297818511843E-4	4.007413292758625E-5	4.218329781851184E-5	0.15003333535110108	5.48382871640654E-5
94-95	0.0022209506301446484	4.323788026397464E-4	4.218329781851184E-5	5.0619957382214214E-5	0.1510077695307087	5.48382871640654E-5
96-97	0.0022272781248174256	4.534704515490023E-4	4.218329781851184E-5	5.48382871640654E-5	0.15187674546577007	5.905661694591659E-5
98-99	0.002250478938617607	4.576887813308535E-4	4.218329781851184E-5	5.48382871640654E-5	0.1527457214008314	6.749327650961895E-5
100-101	0.0022589155981813094	4.7034377067640704E-4	4.218329781851184E-5	5.48382871640654E-5	0.15352611241047387	6.749327650961895E-5
102-103	0.002261024763072235	4.851079249128862E-4	4.218329781851184E-5	5.48382871640654E-5	0.15426432012229782	6.749327650961895E-5
104-105	0.002265243092854086	4.935445844765886E-4	4.218329781851184E-5	5.48382871640654E-5	0.1550468202968312	7.171160629147013E-5
106-107	0.002307426390672598	4.977629142584398E-4	4.218329781851184E-5	5.48382871640654E-5	0.15582299297669183	7.171160629147013E-5
108-109	0.0023158630502363004	5.104179036039933E-4	4.8510792491288625E-5	5.48382871640654E-5	0.15658440150231595	7.592993607332132E-5
110-111	0.0023158630502363004	5.230728929495468E-4	5.2729122273139804E-5	5.48382871640654E-5	0.1574111941395588	7.592993607332132E-5
112-113	0.0023327363693637047	5.27291222731398E-4	5.694745205499099E-5	5.48382871640654E-5	0.15817471183007387	7.803910096424691E-5
114-115	0.0023411730289274076	5.420553769678772E-4	5.905661694591658E-5	5.48382871640654E-5	0.1590247052811169	8.01482658551725E-5
116-117	0.0023559371831638866	5.48382871640654E-4	5.905661694591658E-5	5.48382871640654E-5	0.15989579038106916	8.647576052794928E-5
118-119	0.0023622646778366633	5.526012014225052E-4	6.327494672776777E-5	5.48382871640654E-5	0.1607246921832029	8.858492541887488E-5
120-121	0.0023622646778366633	5.610378609862076E-4	6.327494672776777E-5	5.48382871640654E-5	0.16157046730446406	9.280325520072606E-5
122-123	0.0023622646778366633	5.631470258771332E-4	6.327494672776777E-5	5.48382871640654E-5	0.16235085831410653	1.0123991476442843E-4
124-125	0.00236859217250944	5.715836854408355E-4	6.327494672776777E-5	5.48382871640654E-5	0.16318608761091308	1.075674094372052E-4
126-127	0.0023812471618549933	5.779111801136122E-4	6.327494672776777E-5	5.905661694591658E-5	0.16402553523750146	1.096765743281308E-4
128-129	0.002391792986309622	5.884570045682402E-4	6.327494672776777E-5	5.905661694591658E-5	0.16498309609798167	1.1811323389183316E-4
130-131	0.002410775470327952	5.968936641319426E-4	6.327494672776777E-5	5.905661694591658E-5	0.16594487528824375	1.2654989345553552E-4
132-133	0.0024403037788009105	6.032211588047194E-4	6.327494672776777E-5	5.905661694591658E-5	0.16675901293614104	1.307682232373867E-4
134-135	0.0024635045926010915	6.137669832593474E-4	6.327494672776777E-5	5.905661694591658E-5	0.16759213306805665	1.328773881283123E-4
136-137	0.0024719412521647943	6.285311374958265E-4	6.327494672776777E-5	6.116578183684218E-5	0.16841681654040855	1.413140476920147E-4
138-139	0.0024782687468375706	6.369677970595288E-4	6.327494672776777E-5	6.749327650961895E-5	0.1691803342309236	1.5607820192849382E-4
>>END_MODULE
