##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.2d.R.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24165536
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.72673798752074	37.0	35.0	38.0	33.0	39.0
2	35.3010582922721	35.0	34.0	37.0	33.0	39.0
3	35.459177979747686	35.0	34.0	37.0	33.0	39.0
4	35.51215512041612	36.0	35.0	38.0	33.0	39.0
5	35.42622377587652	36.0	35.0	37.0	33.0	39.0
6	35.386701540574144	35.0	35.0	37.0	33.0	39.0
7	35.41492814394847	35.0	34.0	37.0	33.0	39.0
8	35.54263555337651	36.0	35.0	38.0	33.0	39.0
9	35.498700173668816	36.0	34.0	38.0	33.0	39.0
10-14	35.48151117359863	35.8	34.4	37.8	33.0	39.0
15-19	35.458166729676506	36.0	34.2	38.0	33.0	39.0
20-24	35.44013978419515	36.0	34.2	38.0	33.0	39.0
25-29	35.422188251897246	36.0	34.0	38.0	33.0	39.0
30-34	35.40023088252625	36.0	34.0	38.0	33.0	39.0
35-39	35.3739549414505	36.0	34.0	38.0	33.0	39.0
40-44	35.35388408516989	36.0	34.0	38.0	32.8	39.0
45-49	35.32451104746859	36.0	34.0	38.0	32.0	39.0
50-54	35.29968437695733	36.0	34.0	38.0	32.0	39.0
55-59	35.27372011115334	36.0	34.0	38.0	32.0	39.0
60-64	35.23113918929835	36.0	34.0	38.0	32.0	39.0
65-69	35.20252750859736	35.8	34.0	38.0	32.0	39.0
70-74	35.16890449274537	35.8	34.0	38.0	32.0	39.0
75-79	35.12101064921548	35.4	34.0	37.4	32.0	39.0
80-84	35.07905889610724	35.2	34.0	37.2	32.0	39.0
85-89	35.02607808906039	35.0	34.0	37.0	32.0	39.0
90-94	34.968485515901655	35.0	34.0	37.0	31.4	39.0
95-99	34.9026997621737	35.0	34.0	37.0	31.0	39.0
100-104	34.851126405803704	35.0	34.0	37.0	31.0	39.0
105-109	34.7866045512088	35.0	34.0	37.0	31.0	39.0
110-114	34.7176062140728	35.0	34.0	37.0	31.0	39.0
115-119	34.64737511305356	35.0	34.0	37.0	30.0	39.0
120-124	34.56942005341823	35.0	34.0	37.0	30.0	39.0
125-129	34.489958054313384	35.0	34.0	37.0	30.0	39.0
130-134	34.38544613287287	35.0	34.0	37.0	29.8	39.0
135-139	34.32319804534855	35.0	34.0	37.0	29.8	39.0
140-144	34.189991026890524	35.0	34.0	37.0	29.0	39.0
145-149	34.11311619986414	35.0	34.0	37.0	29.0	39.0
150	34.038951174101825	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	26.0
21	295.0
22	2423.0
23	11799.0
24	36850.0
25	85157.0
26	161629.0
27	267061.0
28	385347.0
29	505564.0
30	646942.0
31	844173.0
32	1128134.0
33	1567981.0
34	2352715.0
35	3998369.0
36	1.0491163E7
37	1679898.0
38	10.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.91614404911192	18.653527900229484	15.822148534176936	31.608179516481655
2	24.035999035982485	25.803971407876077	33.3230969923448	16.83693256379664
3	19.671775540174238	29.702771748989964	30.0090219393437	20.616430771492094
4	23.310544405056856	34.29944198216832	22.34877389022118	20.041239722553637
5	24.974662262819248	35.30953337844441	22.53981455242706	17.175989806309282
6	18.751324199885325	39.09982381520526	22.895093243534927	19.253758741374494
7	20.095709857211528	20.66917944629906	38.24133675330024	20.993773943189176
8	21.396132078344962	23.33816638703979	28.396829269584583	26.868872265030664
9	23.379622119699725	23.215148217693166	28.7895207455775	24.615708917029608
10-14	23.012351143380393	29.23339999576256	25.66546341037087	22.08878545048618
15-19	22.826412788857652	28.32535723602406	27.181899875922472	21.666330099195815
20-24	22.924910086827786	28.31924026017879	27.118931688500513	21.636917964492905
25-29	23.00527412261826	28.25491890599902	27.04952457913617	21.690282392246544
30-34	23.011828084425687	27.995887200681167	27.27363547822817	21.718649236664977
35-39	23.103764163176553	27.975139702882153	27.238360673964447	21.682735459976847
40-44	23.236379279979555	27.734093710977486	27.367940855936325	21.661586153106636
45-49	23.248840000900454	27.564823722511267	27.439297849631807	21.747038426956472
50-54	23.30405334274398	27.53083316670485	27.3958036767734	21.76930981377777
55-59	23.49242656980586	27.417859053488407	27.289383525364386	21.80033085134135
60-64	23.40061234313197	27.38829298054883	27.350667496057195	21.860427180262008
65-69	23.50630749510377	27.321705589315297	27.27921946361959	21.89276745196134
70-74	23.57163937932103	27.28533230134022	27.185125130268162	21.957903189070585
75-79	23.521117843196198	27.376287453338506	27.224051641147128	21.87854306231817
80-84	23.63162563412622	27.413166420144787	27.07961702153017	21.875590924198825
85-89	23.681831017528435	27.35150753535945	27.130519265122032	21.836142181990088
90-94	23.611232128267297	27.29041391840015	27.189641479502047	21.908712473830498
95-99	23.690094024812858	27.385617269155542	27.188212998875755	21.736075707155845
100-104	23.884145586507994	27.363762177673195	26.98161464326717	21.77047759255164
105-109	23.663563048331003	27.518281634789986	27.079155430933998	21.738999885945006
110-114	23.68386118147762	27.525134141448383	26.998761376532265	21.79224330054173
115-119	23.773123012872546	27.50692887589996	27.030394856542806	21.689553254684686
120-124	23.74749974509152	27.426757676717784	27.20637440030298	21.619368177887715
125-129	23.718849417928894	27.521271243828753	27.14775500361763	21.61212433462472
130-134	23.786225793870926	27.41730859056225	27.232381944285216	21.564083671281605
135-139	23.68715264581758	27.43134851219522	27.32429026196646	21.55720858002074
140-144	23.754375652996067	27.556399328365817	27.284658614648567	21.40456640398955
145-149	23.8743291272331	28.137127188074786	26.731454249556062	21.257089435136056
150	23.490118323880754	28.046334250562456	26.89737566756227	21.566171757994525
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	0.0
7	1.5
8	3.5
9	3.5
10	4.5
11	7.5
12	9.5
13	11.5
14	20.0
15	38.5
16	67.5
17	136.0
18	196.5
19	316.5
20	548.5
21	875.0
22	1340.5
23	2248.0
24	3877.0
25	6397.0
26	9922.0
27	15865.5
28	26415.0
29	40388.0
30	55563.5
31	74519.0
32	100728.0
33	135365.0
34	182371.0
35	253881.5
36	356016.5
37	478469.0
38	624274.5
39	811304.5
40	1033291.0
41	1240602.5
42	1420843.5
43	1583402.0
44	1695955.5
45	1746009.5
46	1740524.5
47	1685940.5
48	1584713.5
49	1436540.0
50	1250457.5
51	1048855.5
52	856069.0
53	682316.5
54	531367.0
55	402722.5
56	298499.0
57	219841.5
58	159992.0
59	115038.0
60	80170.0
61	53595.5
62	36455.0
63	25631.0
64	17497.0
65	11469.0
66	8075.5
67	5875.5
68	4697.0
69	3363.0
70	1728.5
71	1046.0
72	578.5
73	421.5
74	345.0
75	214.0
76	137.0
77	54.0
78	12.0
79	0.5
80	0.0
81	0.5
82	1.0
83	0.5
84	0.0
85	0.0
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	8.276249283276813E-7
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	8.276249283276813E-7
110-114	0.0
115-119	0.0
120-124	0.0
125-129	1.6552498566553626E-6
130-134	0.05083024022310119
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4165536E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.28174579209215
#Duplication Level	Percentage of total
1	28.141268115458473
2	10.43149166472612
3	6.943426334590018
4	4.960507913928508
5	3.7694431117168725
6	3.017749177107782
7	2.524815238052663
8	2.131129701036372
9	1.8392864700134433
>10	21.229118897956493
>50	5.491595653309667
>100	6.714416309823454
>500	1.3811122866290177
>1k	1.2462409362979878
>5k	0.07717783823748213
>10k+	0.10122035111581784
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.138124641638406E-6	1.6552498566553625E-5	0.0	0.0	6.165805716041225E-4	0.0
2	8.276249283276813E-6	3.310499713310725E-5	0.0	0.0	0.004800224584300551	0.0
3	2.8966872491468844E-5	3.310499713310725E-5	0.0	0.0	0.0064223694438228065	0.0
4	9.931499139932175E-5	3.724312177474565E-5	0.0	0.0	0.007792088700205119	0.0
5	9.931499139932175E-5	3.724312177474565E-5	0.0	0.0	0.009327332942252967	0.0
6	1.0345311604096015E-4	4.138124641638406E-5	0.0	4.138124641638406E-6	0.011015687796041437	0.0
7	1.1586748996587537E-4	4.965749569966087E-5	0.0	4.138124641638406E-6	0.013026816371877702	0.0
8	1.6552498566553623E-4	5.379562034129928E-5	0.0	4.138124641638406E-6	0.01825326779426701	0.0
9	1.6966311030717465E-4	6.20718696245761E-5	0.0	4.138124641638406E-6	0.024050780417202416	0.0
10-11	1.8621560887372828E-4	6.62099942662145E-5	0.0	4.138124641638406E-6	0.03011106395488186	0.0
12-13	2.1932060600683554E-4	8.276249283276812E-5	0.0	4.138124641638406E-6	0.03584857377051351	0.0
14-15	2.772543509897732E-4	8.483155515358733E-5	0.0	4.138124641638406E-6	0.043996541189899534	0.0
16-17	3.041521611604228E-4	8.690061747440652E-5	0.0	6.207186962457609E-6	0.04951886852416599	0.0
18-19	3.620859061433605E-4	8.690061747440652E-5	0.0	8.276249283276813E-6	0.057230263793859155	0.0
20-21	3.9519090327646775E-4	9.103874211604493E-5	0.0	8.276249283276813E-6	0.06312502234587306	4.138124641638406E-6
22-23	4.3036496273039424E-4	1.0759124068259856E-4	0.0	8.276249283276813E-6	0.06894322559201667	4.138124641638406E-6
24-25	4.882987077133319E-4	1.241437392491522E-4	0.0	8.276249283276813E-6	0.07436623793488378	4.138124641638406E-6
26-27	5.234727671672584E-4	1.365581131740674E-4	0.0	8.276249283276813E-6	0.07819814135304096	4.138124641638406E-6
28-29	6.207186962457609E-4	1.4069623781570582E-4	0.0	8.276249283276813E-6	0.08219350069454284	4.138124641638406E-6
30-31	7.924508688737547E-4	1.489724870989826E-4	4.138124641638406E-6	8.276249283276813E-6	0.08679509529604475	4.138124641638406E-6
32-33	9.331471066894606E-4	1.5931779870307863E-4	4.138124641638406E-6	8.276249283276813E-6	0.09134496333952617	4.138124641638406E-6
34-35	0.0010324620980887824	1.6138686102389784E-4	4.138124641638406E-6	8.276249283276813E-6	0.09453545743822939	4.138124641638406E-6
36-37	0.0010862577184300817	1.6345592334471705E-4	6.207186962457609E-6	8.276249283276813E-6	0.09795768651686435	4.138124641638406E-6
38-39	0.001131777089488104	1.7380123494881305E-4	8.276249283276813E-6	8.276249283276813E-6	0.10132818903747884	6.207186962457609E-6
40-41	0.0011793655228669458	1.9035373351536668E-4	8.276249283276813E-6	8.276249283276813E-6	0.10397245068348576	1.0345311604096015E-5
42-43	0.0012083323953584147	2.0276810744028188E-4	8.276249283276813E-6	8.276249283276813E-6	0.10707190604007294	1.2414373924915218E-5
44-45	0.001253851766416437	2.1518248136519712E-4	8.276249283276813E-6	1.2414373924915218E-5	0.10951753770328124	1.4483436245734422E-5
46-47	0.0013345451969283858	2.5035654081912356E-4	8.276249283276813E-6	1.2414373924915218E-5	0.11189075218526086	1.6552498566553625E-5
48-49	0.0014007551911946006	2.5242560313994274E-4	1.0345311604096015E-5	1.2414373924915218E-5	0.11418120417440772	2.069062320819203E-5
50-51	0.001448343624573442	2.5863279010240035E-4	1.2414373924915218E-5	1.2414373924915218E-5	0.11625026649522692	2.069062320819203E-5
52-53	0.0014917939333106453	2.669090393856772E-4	1.2414373924915218E-5	1.2414373924915218E-5	0.11829450006819629	2.2759685529011233E-5
54-55	0.0015166226811604757	2.834615379522308E-4	1.2414373924915218E-5	1.2414373924915218E-5	0.12018562302942505	2.4828747849830436E-5
56-57	0.0015931779870307864	2.917377872355076E-4	1.2414373924915218E-5	1.2414373924915218E-5	0.12202501943263332	2.4828747849830436E-5
58-59	0.0016655951682594584	3.0208309883960363E-4	1.2414373924915218E-5	1.2414373924915218E-5	0.1239202805185037	2.4828747849830436E-5
60-61	0.0017566339103755033	3.16566535085338E-4	1.2414373924915218E-5	1.2414373924915218E-5	0.1253189666473775	2.4828747849830436E-5
62-63	0.0017793935959045145	3.351880959727109E-4	1.2414373924915218E-5	1.2414373924915218E-5	0.12723284929413525	2.689781017064964E-5
64-65	0.0018021532814335257	3.600168438225413E-4	1.2414373924915218E-5	1.2414373924915218E-5	0.12892327321024455	2.8966872491468844E-5
66-67	0.0018869848365871131	3.8691465399319096E-4	1.2414373924915218E-5	1.6552498566553625E-5	0.13024126590860638	2.8966872491468844E-5
68-69	0.0019283660830034972	3.9312184095564856E-4	1.2414373924915218E-5	1.6552498566553625E-5	0.13146615080253132	3.103593481228805E-5
70-71	0.001959402017815785	4.096743395222022E-4	1.2414373924915218E-5	1.6552498566553625E-5	0.13266620694860648	3.310499713310725E-5
72-73	0.001994576077269712	4.200196511262982E-4	1.2414373924915218E-5	1.6552498566553625E-5	0.13353314406102973	3.724312177474565E-5
74-75	0.002021473887440361	4.220887134471174E-4	1.2414373924915218E-5	1.6552498566553625E-5	0.1346814736490844	3.724312177474565E-5
76-77	0.002044233572969373	4.28295900409575E-4	1.2414373924915218E-5	1.6552498566553625E-5	0.13587118448355542	3.724312177474565E-5
78-79	0.0020959601309898527	4.407102743344902E-4	1.2414373924915218E-5	1.6552498566553625E-5	0.13730918279652476	3.724312177474565E-5
80-81	0.0021187198165188636	4.4898652361776705E-4	1.2414373924915218E-5	1.6552498566553625E-5	0.13856717268758284	3.724312177474565E-5
82-83	0.0021352723150854175	4.6760808450513986E-4	1.8621560887372826E-5	1.6552498566553625E-5	0.14014379817604705	3.724312177474565E-5
84-85	0.002168377312218525	4.7381527146759747E-4	2.4828747849830436E-5	1.6552498566553625E-5	0.1415135174324294	3.724312177474565E-5
86-87	0.0021787226238226204	4.841605830716935E-4	2.4828747849830436E-5	1.6552498566553625E-5	0.14295358480771955	3.724312177474565E-5
88-89	0.0021952751223891743	4.841605830716935E-4	2.4828747849830436E-5	1.6552498566553625E-5	0.14448055280048414	3.724312177474565E-5
90-91	0.0022138966832765472	5.007130816382471E-4	2.4828747849830436E-5	1.6552498566553625E-5	0.14539507834628623	4.138124641638406E-5
92-93	0.002222172932559824	5.007130816382471E-4	2.8966872491468844E-5	2.069062320819203E-5	0.1464461620052624	4.345030873720326E-5
94-95	0.0022387254311263777	5.007130816382471E-4	2.8966872491468844E-5	2.069062320819203E-5	0.14752207441208837	4.5519371058022466E-5
96-97	0.002251139805051293	5.027821439590663E-4	2.8966872491468844E-5	2.4828747849830436E-5	0.14849246464055255	4.5519371058022466E-5
98-99	0.002265623241297027	5.048512062798855E-4	3.103593481228805E-5	2.4828747849830436E-5	0.14940699018635467	4.758843337884167E-5
100-101	0.0022780376152219425	5.110583932423432E-4	3.310499713310725E-5	2.4828747849830436E-5	0.15027599636109873	5.1726558020480076E-5
102-103	0.002292521051467677	5.131274555631623E-4	3.310499713310725E-5	2.8966872491468844E-5	0.1511036212894264	5.793374498293769E-5
104-105	0.002307004487713411	5.317490164505351E-4	3.310499713310725E-5	2.8966872491468844E-5	0.15199124902505784	5.793374498293769E-5
106-107	0.002321487923959146	5.462324526962696E-4	3.310499713310725E-5	3.103593481228805E-5	0.1527423186475152	5.793374498293769E-5
108-109	0.0023256260486007843	5.524396396587272E-4	3.724312177474565E-5	3.310499713310725E-5	0.15357615076280534	5.793374498293769E-5
110-111	0.002342178547167338	5.689921382252809E-4	4.138124641638406E-5	3.310499713310725E-5	0.15437687788096238	6.20718696245761E-5
112-113	0.0023504547964506147	5.896827614334728E-4	4.138124641638406E-5	3.310499713310725E-5	0.15509484250628663	6.20718696245761E-5
114-115	0.0023504547964506147	6.000280730375689E-4	4.138124641638406E-5	3.310499713310725E-5	0.1558769480635563	6.62099942662145E-5
116-117	0.0023504547964506147	6.062352600000265E-4	4.138124641638406E-5	3.310499713310725E-5	0.15671078017884643	8.483155515358733E-5
118-119	0.0023545929210922532	6.145115092833033E-4	4.138124641638406E-5	3.310499713310725E-5	0.15744736636505807	9.724592907850254E-5
120-121	0.0023587310457338913	6.165805716041225E-4	4.138124641638406E-5	3.310499713310725E-5	0.15831637253980213	1.0552217836177935E-4
122-123	0.0023587310457338913	6.186496339249417E-4	4.138124641638406E-5	3.310499713310725E-5	0.15901778466655986	1.0759124068259856E-4
124-125	0.0023608001080547107	6.227877585665801E-4	4.138124641638406E-5	3.310499713310725E-5	0.159874376467379	1.1586748996587537E-4
126-127	0.0023690763573379877	6.289949455290377E-4	4.138124641638406E-5	3.310499713310725E-5	0.1608778716929763	1.1586748996587537E-4
128-129	0.00238769791822536	6.41409319453953E-4	4.138124641638406E-5	3.310499713310725E-5	0.16175101599236202	1.1793655228669458E-4
130-131	0.0024001122921502756	6.538236933788682E-4	4.345030873720326E-5	3.310499713310725E-5	0.16269450841065558	1.2621280156997137E-4
132-133	0.0024332172892833825	6.620999426621449E-4	4.5519371058022466E-5	3.310499713310725E-5	0.16345592334471704	1.2828186389079058E-4
134-135	0.0024828747849830438	6.641690049829642E-4	4.5519371058022466E-5	3.5174059453926454E-5	0.16415940453379557	1.303509262116098E-4
136-137	0.0024870129096246823	6.662380673037834E-4	4.5519371058022466E-5	3.724312177474565E-5	0.16502634164621882	1.448343624573442E-4
138-139	0.0024973582212287782	6.82790565870337E-4	4.5519371058022466E-5	3.724312177474565E-5	0.16584775938758403	1.489724870989826E-4
>>END_MODULE
