##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.2d.R.3.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23338379
Total Bases	3.5 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.75229856366631	36.0	34.0	38.0	30.0	39.0
2	34.64950740580569	35.0	34.0	37.0	31.0	39.0
3	34.86173324205593	35.0	34.0	38.0	31.0	39.0
4	34.803082681963474	35.0	34.0	38.0	30.0	39.0
5	34.686637405279946	35.0	34.0	37.0	31.0	39.0
6	34.68154137868787	35.0	34.0	37.0	31.0	39.0
7	34.69334678299637	35.0	34.0	37.0	32.0	39.0
8	34.82642440591097	35.0	34.0	37.0	31.0	39.0
9	34.805107929732394	35.0	34.0	37.0	31.0	39.0
10-14	34.784693418510344	35.0	34.0	37.0	31.0	39.0
15-19	34.7874340972867	35.0	34.0	37.0	31.0	39.0
20-24	34.79263056787277	35.0	34.0	37.0	31.0	39.0
25-29	34.782022753165506	35.0	34.0	37.0	31.0	39.0
30-34	34.76959084433413	35.0	34.0	37.0	31.0	39.0
35-39	34.766659149720724	35.0	34.0	37.0	31.0	39.0
40-44	34.69742937159431	35.0	34.0	37.0	31.0	39.0
45-49	34.611771357385194	35.0	34.0	37.0	31.0	39.0
50-54	34.56951593767503	35.0	34.0	37.0	30.4	39.0
55-59	34.56259497714044	35.0	34.0	37.0	30.2	39.0
60-64	34.53538420127636	35.0	34.0	37.0	30.0	39.0
65-69	34.51742028870129	35.0	34.0	37.0	30.0	39.0
70-74	34.48998633538345	35.0	34.0	37.0	30.0	39.0
75-79	34.4724672351923	35.0	34.0	37.0	30.0	39.0
80-84	34.44984578406238	35.0	34.0	37.0	30.0	39.0
85-89	34.42896021184676	35.0	34.0	37.0	30.0	39.0
90-94	34.4026336961963	35.0	34.0	37.0	30.0	39.0
95-99	34.37631273363073	35.0	34.0	37.0	30.0	39.0
100-104	34.33785653236671	35.0	34.0	37.0	30.0	39.0
105-109	34.289945227129955	35.0	34.0	37.0	30.0	39.0
110-114	34.256317167529076	35.0	34.0	37.0	30.0	39.0
115-119	34.2112924809388	35.0	34.0	37.0	30.0	39.0
120-124	34.17483877522085	35.0	34.0	37.0	29.0	39.0
125-129	34.13137919304507	35.0	34.0	37.0	29.0	39.0
130-134	34.076296464291715	35.0	34.0	37.0	29.0	39.0
135-139	34.03293306703092	35.0	34.0	37.0	29.0	39.0
140-144	33.99589087999642	35.0	34.0	37.0	29.0	39.0
145-149	33.93048198420293	35.0	34.0	37.0	29.0	39.0
150	33.89653321680996	35.0	34.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	22.0
22	440.0
23	4308.0
24	25694.0
25	94690.0
26	227538.0
27	399129.0
28	576026.0
29	742651.0
30	919743.0
31	1143882.0
32	1452953.0
33	1899876.0
34	2606891.0
35	4045173.0
36	8213089.0
37	986266.0
38	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.322245142123144	10.853193906903687	14.733445246740956	38.091115704232216
2	22.342758330535002	12.803368467671225	36.72492891199956	28.128944289794212
3	21.290608832281098	17.27270954927648	28.32716002170675	33.109521596735675
4	26.34045249691613	24.982804068270582	22.218120726431227	26.458622708382062
5	25.85347855454528	30.586383660351284	24.44485416549192	19.115283619611514
6	19.272368316075184	36.68676863390405	25.57203068289415	18.468832367126616
7	16.016662539694877	24.559528422309747	40.550576457047775	18.8732325809476
8	18.573016516687392	24.350699210089356	31.50167175586126	25.574612517361995
9	19.696386380749246	22.930141539158708	32.71827691995496	24.65519516013709
10-14	20.28702669687071	29.19898281671455	27.135646736141044	23.378343750273693
15-19	20.434280306914214	27.85503595253327	27.97430685160707	23.73637688894544
20-24	20.58020271268049	28.017116972468575	27.680452821377198	23.722227493473742
25-29	20.57945895858681	28.210557182609584	27.5579002455655	23.6520836132381
30-34	20.539439432075024	27.942466569655277	27.599441995259866	23.918652003009836
35-39	20.616288284944424	27.910334836069307	27.651886678832042	23.82149020015423
40-44	20.664135074156757	27.981185503879587	27.55517291736533	23.799506504598334
45-49	20.74352836856489	27.768968072241133	27.53026962395116	23.957233935242815
50-54	20.869912928135555	27.749447353337608	27.606943029361013	23.77369668916582
55-59	20.86422803339211	27.77352720274564	27.539244301300315	23.823000462561932
60-64	20.80443872825763	27.692320533516966	27.530967047488637	23.97227369073677
65-69	20.928252939868898	27.602559236677305	27.616939031375832	23.852248792077962
70-74	21.022416863105597	27.547808266349655	27.606360954380875	23.823413916163876
75-79	21.036677060561768	27.449734674934888	27.587403567160667	23.926184697342677
80-84	21.097682509263997	27.443331032452768	27.572066996861366	23.886919461421872
85-89	21.073810190790223	27.433883958312936	27.636848252332708	23.85545759856413
90-94	21.062370352008557	27.352689660998564	27.593193993393843	23.991745993599032
95-99	21.152083089778063	27.270959908875636	27.688124776579727	23.888832224766578
100-104	21.193814750878197	27.333838592058363	27.560971924822102	23.91137473224134
105-109	21.169023425008135	27.168223658587987	27.64226560974143	24.02048730666245
110-114	21.250332393220045	27.164079748321235	27.69013556330228	23.89545229515644
115-119	21.285365904741685	27.16776905366339	27.663466996963997	23.88339804463093
120-124	21.23766192676699	27.086945560110394	27.663584983465828	24.011807529656796
125-129	21.281643923500475	26.997295335927586	27.736900282827175	23.98416045774476
130-134	21.363227204676942	27.09700947025367	27.62179915109644	23.917964173972955
135-139	21.35407978352806	26.945602286421284	27.716099205885293	23.984218724165366
140-144	21.403537881473472	26.876139664398924	27.852375343339546	23.86794711078806
145-149	21.40195409154623	27.464141034166563	27.494501385433118	23.639403488854093
150	21.266083311388027	27.027367263164848	27.59099390264647	24.115555522800655
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	2.0
11	4.5
12	10.0
13	21.0
14	35.5
15	66.5
16	147.5
17	288.5
18	567.0
19	1042.5
20	1943.5
21	3328.5
22	5270.0
23	8475.0
24	13505.0
25	21485.5
26	32193.5
27	45187.5
28	61712.0
29	87107.0
30	118393.5
31	150367.0
32	182240.0
33	218953.0
34	258462.5
35	303105.5
36	371839.5
37	465965.0
38	579388.5
39	719682.0
40	881628.0
41	1043389.0
42	1208564.5
43	1379425.5
44	1520743.5
45	1606073.5
46	1630839.5
47	1593762.5
48	1511040.5
49	1388431.0
50	1226952.5
51	1051138.0
52	873467.0
53	698907.0
54	540898.5
55	406979.0
56	301857.5
57	224721.0
58	169358.0
59	127241.0
60	90008.5
61	64133.0
62	46706.0
63	30232.0
64	19471.5
65	14910.0
66	14164.5
67	10587.0
68	5105.5
69	2546.0
70	1909.0
71	1280.0
72	586.5
73	239.0
74	109.0
75	74.0
76	51.0
77	39.0
78	17.0
79	1.5
80	0.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.156244099043897E-4
2	2.2280896201060064E-4
3	5.998702823362325E-5
4	4.284787730973089E-5
5	2.9993514116811625E-5
6	1.4139799512211197E-4
7	1.7139150923892356E-4
8	1.8424587243184284E-4
9	1.3711320739113886E-4
10-14	2.0652676863290288E-4
15-19	3.393551882930687E-4
20-24	1.979571931709567E-4
25-29	3.6077912694793414E-4
30-34	9.683620271999183E-4
35-39	0.0010120668620558436
40-44	0.0012391606117974174
45-49	9.846442205776159E-4
50-54	5.990133247900379E-4
55-59	4.2333702782014124E-4
60-64	2.339494101111307E-4
65-69	3.27357782646344E-4
70-74	3.530665090321826E-4
75-79	2.7336945723608307E-4
80-84	2.905086081599755E-4
85-89	3.7191957504846416E-4
90-94	3.787752354180211E-4
95-99	3.265008251001494E-4
100-104	4.490457542059798E-4
105-109	2.776542449670562E-4
110-114	2.442329006654661E-4
115-119	2.2880766483396298E-4
120-124	2.1852417427962756E-4
125-129	1.1911709892105188E-4
130-134	1.6710672150795048E-4
135-139	1.619649762307828E-4
140-144	1.679636790541451E-4
145-149	1.9538632053237286E-4
150	2.442329006654661E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.3338379E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.73532850346281
#Duplication Level	Percentage of total
1	39.11360890988115
2	10.90071914428529
3	6.429377296235186
4	4.488322259990327
5	3.3921839138190766
6	2.6921262901982095
7	2.112771675831964
8	1.865258224785714
9	1.6244397865231086
>10	17.761691416823606
>50	4.502964121570804
>100	4.411837424328064
>500	0.39206244155130954
>1k	0.31263709417609953
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	2.1423938654865448E-5	0.0	4.2847877309730894E-6	1.4568278285308504E-4	2.1423938654865448E-5
2	0.0	9.426533008140797E-5	0.0	1.7139150923892358E-5	0.0019024457525520517	4.2847877309730896E-5
3	0.0	1.242588441982196E-4	0.0	1.7139150923892358E-5	0.0030807623785696512	7.712617915751561E-5
4	2.9993514116811625E-5	1.4996757058405812E-4	4.2847877309730894E-6	1.7139150923892358E-5	0.00445189445248104	8.998054235043488E-5
5	3.4278301847784716E-5	1.5425235831503122E-4	4.2847877309730894E-6	2.1423938654865448E-5	0.005690198106732262	1.0283490554335414E-4
6	4.2847877309730896E-5	1.5425235831503122E-4	4.2847877309730894E-6	2.1423938654865448E-5	0.006731401525358723	1.0711969327432723E-4
7	4.7132665040703986E-5	1.8853066016281595E-4	4.2847877309730894E-6	2.1423938654865448E-5	0.007978274755071893	1.0711969327432723E-4
8	5.998702823362325E-5	2.2280896201060064E-4	4.2847877309730894E-6	2.1423938654865448E-5	0.010060681592324814	1.242588441982196E-4
9	7.712617915751561E-5	2.2280896201060064E-4	4.2847877309730894E-6	2.1423938654865448E-5	0.013372822508367013	1.3282841966016579E-4
10-11	1.3497081352565232E-4	2.2709374974157375E-4	4.2847877309730894E-6	2.785112025132508E-5	0.017993966076221488	1.9067305402830248E-4
12-13	2.0566981108670828E-4	2.2923614360706028E-4	4.2847877309730894E-6	3.4278301847784716E-5	0.021621038890490208	2.3352093133803336E-4
14-15	2.2280896201060064E-4	2.485176883964392E-4	4.2847877309730894E-6	3.4278301847784716E-5	0.026057936585912843	2.677992331858181E-4
16-17	2.8065359637873737E-4	2.5922965772387193E-4	4.2847877309730894E-6	3.4278301847784716E-5	0.030644801851919534	2.742264147822777E-4
18-19	3.3635583688138753E-4	2.7636880864776424E-4	4.2847877309730894E-6	3.4278301847784716E-5	0.03478390680003954	2.785112025132508E-4
20-21	3.684917448636857E-4	3.1707429209200865E-4	4.2847877309730894E-6	3.4278301847784716E-5	0.03855452000329586	2.827959902442239E-4
22-23	4.1133962217341656E-4	3.277862614194414E-4	4.2847877309730894E-6	3.642069571327126E-5	0.04260150201519994	3.020775350336028E-4
24-25	4.777538320034995E-4	3.3635583688138753E-4	4.2847877309730894E-6	3.8563089578757806E-5	0.047130522646838494	3.0421992889908937E-4
26-27	5.377408602371227E-4	3.3849823074687404E-4	4.2847877309730894E-6	4.499027117521744E-5	0.05161026821957086	3.1707429209200865E-4
28-29	5.78446343681367E-4	3.3849823074687404E-4	4.2847877309730894E-6	4.927505890619053E-5	0.05603431155180058	3.384982307468741E-4
30-31	6.298637964530442E-4	3.3849823074687404E-4	4.2847877309730894E-6	5.998702823362325E-5	0.06025268507294358	3.642069571327126E-4
32-33	6.662844921663154E-4	3.3849823074687404E-4	4.2847877309730894E-6	5.998702823362325E-5	0.06415398430199458	4.070548344424435E-4
34-35	7.177019449379925E-4	3.3849823074687404E-4	4.2847877309730894E-6	6.21294220991098E-5	0.06785175611382435	4.1133962217341656E-4
36-37	7.348410958618848E-4	3.449254123433337E-4	4.2847877309730894E-6	6.427181596459634E-5	0.07143598105078335	4.156244099043897E-4
38-39	7.669770038441831E-4	3.642069571327126E-4	4.2847877309730894E-6	6.427181596459634E-5	0.07583003086889625	4.220515915008493E-4
40-41	8.1839445661586E-4	3.642069571327126E-4	4.2847877309730894E-6	6.427181596459634E-5	0.07907361518124288	4.263363792318224E-4
42-43	8.269640320778063E-4	3.684917448636857E-4	4.2847877309730894E-6	6.427181596459634E-5	0.08224864288989395	4.284787730973089E-4
44-45	8.740966971185103E-4	3.8563089578757805E-4	4.2847877309730894E-6	6.641420983008288E-5	0.0866491198896033	4.284787730973089E-4
46-47	9.3622611921762E-4	3.963428651150108E-4	4.2847877309730894E-6	7.069899756105598E-5	0.09098318267948258	4.32763560828282E-4
48-49	9.705044210654047E-4	4.0062765284598384E-4	4.2847877309730894E-6	7.712617915751561E-5	0.09483949163735836	4.4776031788668785E-4
50-51	9.83358784258324E-4	4.027700467114704E-4	8.569575461946179E-6	7.712617915751561E-5	0.09927853172664648	4.541874994831475E-4
52-53	0.001026206661568055	4.027700467114704E-4	8.569575461946179E-6	7.712617915751561E-5	0.1033340833140125	4.691842565415533E-4
54-55	0.0010583425695503532	4.027700467114704E-4	8.569575461946179E-6	7.712617915751561E-5	0.10681333095156266	4.79896225868986E-4
56-57	0.001096905659129111	4.070548344424435E-4	8.569575461946179E-6	7.926857302300216E-5	0.11118167204328973	4.884658013309322E-4
58-59	0.0011418959303043283	4.1990919763536277E-4	8.569575461946179E-6	9.640772394689451E-5	0.11543860865401148	4.906081951964187E-4
60-61	0.0012018829585379515	4.2419398536633584E-4	8.569575461946179E-6	1.0283490554335414E-4	0.11896284656273685	4.970353767928784E-4
62-63	0.0012618699867715747	4.2419398536633584E-4	1.2854363192919268E-5	1.0283490554335414E-4	0.12236068323339852	4.99177770658365E-4
64-65	0.0013025754702158192	4.2419398536633584E-4	1.2854363192919268E-5	1.0283490554335414E-4	0.127532422024683	5.013201645238514E-4
66-67	0.0013068602579467924	4.3704834855925513E-4	1.2854363192919268E-5	1.0283490554335414E-4	0.1318364913004455	5.056049522548245E-4
68-69	0.0013347113781981175	4.5847228721412057E-4	1.2854363192919268E-5	1.0283490554335414E-4	0.13466016641515677	5.163169215822573E-4
70-71	0.0013775592555078484	4.8203861973447257E-4	1.2854363192919268E-5	1.0283490554335414E-4	0.13738100662432468	5.184593154477439E-4
72-73	0.0014246919205485524	4.884658013309322E-4	1.2854363192919268E-5	1.0283490554335414E-4	0.13997330320156343	5.227441031787169E-4
74-75	0.0014696821917237696	4.906081951964187E-4	1.2854363192919268E-5	1.0283490554335414E-4	0.1430819167003844	5.2702889090969E-4
76-77	0.0014996757058405813	5.03462558389338E-4	1.2854363192919268E-5	1.0926208713981378E-4	0.14552424570703903	5.2702889090969E-4
78-79	0.0015425235831503122	5.098897399857976E-4	1.2854363192919268E-5	1.4568278285308504E-4	0.148157247767722	5.291712847751766E-4
80-81	0.0015746594911326102	5.141745277167707E-4	1.2854363192919268E-5	1.5425235831503122E-4	0.15088879994621734	5.313136786406631E-4
82-83	0.001617507368442341	5.141745277167707E-4	1.2854363192919268E-5	1.5425235831503122E-4	0.1533804040117782	5.355984663716362E-4
84-85	0.0016496432764246394	5.227441031787169E-4	1.2854363192919268E-5	1.5425235831503122E-4	0.1558141634429709	5.398832541026093E-4
86-87	0.0017331966371786148	5.2702889090969E-4	1.2854363192919268E-5	1.5425235831503122E-4	0.15803154109374948	5.398832541026093E-4
88-89	0.001778186908353832	5.2702889090969E-4	1.2854363192919268E-5	1.5425235831503122E-4	0.16013965665738825	5.398832541026093E-4
90-91	0.0018188923917980765	5.2702889090969E-4	1.2854363192919268E-5	1.5425235831503122E-4	0.16308116343470128	5.398832541026093E-4
92-93	0.0018810218138971863	5.291712847751766E-4	1.2854363192919268E-5	1.5425235831503122E-4	0.1657055959199223	5.398832541026093E-4
94-95	0.0019410088421308095	5.398832541026093E-4	1.2854363192919268E-5	1.5425235831503122E-4	0.1687263712702583	5.484528295645554E-4
96-97	0.0020288469906157578	5.570224050265016E-4	1.2854363192919268E-5	1.5425235831503122E-4	0.17114513394439262	5.484528295645554E-4
98-99	0.0020931188065803543	5.741615559503939E-4	1.2854363192919268E-5	1.585371460460043E-4	0.173154699390219	5.484528295645554E-4
100-101	0.0021852417427962755	5.913007068742863E-4	1.2854363192919268E-5	1.585371460460043E-4	0.17531209001276396	5.50595223430042E-4
102-103	0.002206665681451141	5.913007068742863E-4	1.2854363192919268E-5	1.585371460460043E-4	0.17711598564750364	5.527376172955286E-4
104-105	0.002240943983298926	5.998702823362325E-4	1.2854363192919268E-5	1.628219337769774E-4	0.17880204961964152	5.570224050265016E-4
106-107	0.0022795070728776835	5.998702823362325E-4	1.2854363192919268E-5	1.628219337769774E-4	0.1803510003843883	5.613071927574747E-4
108-109	0.0023244973440529008	5.998702823362325E-4	1.2854363192919268E-5	1.628219337769774E-4	0.18180997060678467	5.613071927574747E-4
110-111	0.0023780571906900647	6.062974639326922E-4	1.2854363192919268E-5	1.628219337769774E-4	0.1832368049211987	5.613071927574747E-4
112-113	0.0024444714005201476	6.084398577981787E-4	1.2854363192919268E-5	1.628219337769774E-4	0.1848907329853543	5.613071927574747E-4
114-115	0.0024916040655608516	6.148670393946383E-4	1.2854363192919268E-5	1.6924911537343705E-4	0.18673104931580725	5.613071927574747E-4
116-117	0.002560160669256421	6.170094332601249E-4	1.2854363192919268E-5	1.7996108470086977E-4	0.18824572177870624	5.613071927574747E-4
118-119	0.002622290091355531	6.470029473769365E-4	1.2854363192919268E-5	1.821034785663563E-4	0.18974325509068132	5.613071927574747E-4
120-121	0.0027144130275714522	6.641420983008288E-4	1.2854363192919268E-5	1.8424587243184284E-4	0.19120008291921217	5.613071927574747E-4
122-123	0.0028686653858864836	6.684268860318019E-4	1.2854363192919268E-5	1.863882662973294E-4	0.19259906611337488	5.613071927574747E-4
124-125	0.0029629307159678916	6.684268860318019E-4	1.2854363192919268E-5	1.8853066016281595E-4	0.19404089718484735	5.613071927574747E-4
126-127	0.0030164905626050547	6.705692798972885E-4	1.2854363192919268E-5	1.8853066016281595E-4	0.19545701952993394	5.613071927574747E-4
128-129	0.0030743351969731918	6.769964614937481E-4	1.2854363192919268E-5	1.9495784175927556E-4	0.19671888951670552	5.634495866229613E-4
130-131	0.003138607012937788	6.855660369556942E-4	1.2854363192919268E-5	2.013850233557352E-4	0.19797218992801513	5.655919804884478E-4
132-133	0.0032457267062121153	6.898508246866674E-4	1.2854363192919268E-5	2.013850233557352E-4	0.1990883771319336	5.655919804884478E-4
134-135	0.003406406246123606	6.898508246866674E-4	1.2854363192919268E-5	2.013850233557352E-4	0.20005245437140257	5.655919804884478E-4
136-137	0.0035649433921696103	6.941356124176405E-4	1.7139150923892358E-5	2.013850233557352E-4	0.2011643567875901	5.677343743539343E-4
138-139	0.0036249304204032337	7.069899756105597E-4	1.7139150923892358E-5	2.0352741722122174E-4	0.2022741168099121	5.698767682194209E-4
>>END_MODULE
