##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.2d.R.3.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23338379
Total Bases	3.5 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.6957631033415	37.0	35.0	38.0	33.0	39.0
2	35.305753711515266	35.0	34.0	37.0	33.0	39.0
3	35.46300285036934	35.0	34.0	37.0	33.0	39.0
4	35.51029760892991	36.0	35.0	38.0	33.0	39.0
5	35.42291951810363	36.0	35.0	37.0	33.0	39.0
6	35.382137808285655	35.0	35.0	37.0	33.0	39.0
7	35.4156325081532	35.0	34.0	37.0	33.0	39.0
8	35.49625653092702	36.0	35.0	38.0	33.0	39.0
9	35.49738291592574	36.0	34.0	38.0	33.0	39.0
10-14	35.48414479857406	35.8	34.6	37.8	33.0	39.0
15-19	35.45681284034337	36.0	35.0	38.0	33.0	39.0
20-24	35.44198808323406	36.0	34.2	38.0	33.0	39.0
25-29	35.421105021904054	36.0	34.0	38.0	33.0	39.0
30-34	35.40104940450234	36.0	34.0	38.0	33.0	39.0
35-39	35.37349601701129	36.0	34.0	38.0	33.0	39.0
40-44	35.35511266656523	36.0	34.0	38.0	33.0	39.0
45-49	35.32509586891189	36.0	34.0	38.0	32.0	39.0
50-54	35.302691390863096	36.0	34.0	38.0	32.0	39.0
55-59	35.27490447387113	36.0	34.0	38.0	32.0	39.0
60-64	35.23402330556034	36.0	34.0	38.0	32.0	39.0
65-69	35.205235265054185	36.0	34.0	38.0	32.0	39.0
70-74	35.17482850886945	36.0	34.0	37.8	32.0	39.0
75-79	35.12682745446888	35.6	34.0	37.2	32.0	39.0
80-84	35.085397713354475	35.4	34.0	37.0	32.0	39.0
85-89	35.035123450518995	35.0	34.0	37.0	32.0	39.0
90-94	34.97824030537854	35.0	34.0	37.0	31.6	39.0
95-99	34.914087889308846	35.0	34.0	37.0	31.0	39.0
100-104	34.863625138661085	35.0	34.0	37.0	31.0	39.0
105-109	34.80140666153377	35.0	34.0	37.0	31.0	39.0
110-114	34.72605991187305	35.0	34.0	37.0	31.0	39.0
115-119	34.66396955846848	35.0	34.0	37.0	30.4	39.0
120-124	34.58879366900332	35.0	34.0	37.0	30.0	39.0
125-129	34.51096616435957	35.0	34.0	37.0	30.0	39.0
130-134	34.40794989232114	35.0	34.0	37.0	30.0	39.0
135-139	34.3478015589686	35.0	34.0	37.0	29.8	39.0
140-144	34.21750638294117	35.0	34.0	37.0	29.0	39.0
145-149	34.14162518313719	35.0	34.0	37.0	29.0	39.0
150	34.07247384233498	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	25.0
21	241.0
22	2083.0
23	9827.0
24	31665.0
25	74942.0
26	147432.0
27	250590.0
28	368326.0
29	488197.0
30	626030.0
31	817551.0
32	1091915.0
33	1525449.0
34	2289224.0
35	3900975.0
36	1.0094406E7
37	1619494.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.403897545754994	18.426913882922204	15.841053056855406	31.32813551446739
2	24.4942290122206	25.42014164736977	32.94974342476827	17.135885915641357
3	20.130639750087184	29.320236851068366	29.693219053474106	20.855904345370345
4	23.805912141541622	33.89040858407518	22.144794203573436	20.15888507080976
5	25.422656817767848	35.09503380676095	22.271396826660496	17.210912548810693
6	19.081685150455392	39.10511094193817	22.587716996111855	19.22548691149458
7	20.48601147491863	20.624877160491735	37.860153012340746	21.028958352248885
8	21.775585130695507	23.305177394000193	28.16400411046257	26.75523336484173
9	23.685998072102606	23.11956198843116	28.61936983712536	24.57507010234087
10-14	23.303525264574077	29.15974437778857	25.462783216116108	22.073947141521245
15-19	23.113211076056313	28.31987688605108	26.951116870627562	21.615795167265045
20-24	23.201469133738893	28.37611815285029	26.845368309427148	21.57704440398367
25-29	23.27592160535228	28.33087593615649	26.78787845548313	21.605324003008093
30-34	23.258940991574438	28.07978908903656	27.020468730926	21.640801188463005
35-39	23.331236043964083	28.08297488155411	26.990181742959642	21.595607331522164
40-44	23.47439211609341	27.85661334919619	27.09683307482495	21.572161459885454
45-49	23.464849893816535	27.69754317555645	27.184013079914422	21.653593850712596
50-54	23.52017678691395	27.687563904931018	27.14792059894134	21.644338709213695
55-59	23.69479388435675	27.58543170457554	27.04771912393744	21.672055287130267
60-64	23.587094887781195	27.57348828725423	27.114622656526404	21.724794168438176
65-69	23.688822604174867	27.4966457610445	27.06270045576002	21.751831179020616
70-74	23.765828809275913	27.463281832898506	26.989278047117153	21.781611310708428
75-79	23.67624246739673	27.5248508047624	27.058904133830374	21.740002594010495
80-84	23.79150668519009	27.573558557773016	26.911952196851374	21.72298256018552
85-89	23.83023345366017	27.505783499359577	26.955914118971158	21.708068928009098
90-94	23.74913119500828	27.45316225649744	27.036975678876203	21.760730869618072
95-99	23.809204776468878	27.555258465510896	27.037823209896654	21.597713548123572
100-104	23.98855121857435	27.517475828119853	26.857474548682237	21.63649840462356
105-109	23.767864083448124	27.646567912878613	26.979302204321904	21.60626579935136
110-114	23.801120355840748	27.639309113612498	26.917668559034315	21.641901971512436
115-119	23.872692272243928	27.611328104663997	26.950225634779517	21.565753988312554
120-124	23.817268747047674	27.53401448775868	27.136315055439926	21.51240170975372
125-129	23.791039847206523	27.628677446952743	27.092025775157957	21.488256930682777
130-134	23.86464803988711	27.526158277364875	27.160694428467757	21.448499254280254
135-139	23.745600529887707	27.537480024105186	27.266187829636223	21.450731616370884
140-144	23.793000362193105	27.66385446050045	27.230062550616736	21.31308262668971
145-149	23.9175017253769	28.195600045744396	26.723434391051754	21.163463837826953
150	23.534955019798076	28.137146971518455	26.866793105039555	21.461104903643907
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	2.5
9	2.5
10	5.0
11	8.0
12	7.5
13	8.5
14	17.0
15	32.0
16	53.5
17	83.5
18	146.5
19	261.5
20	419.0
21	653.0
22	1041.5
23	1842.5
24	3193.5
25	5305.0
26	8285.0
27	13275.5
28	21990.0
29	33371.5
30	46609.0
31	63572.0
32	87141.0
33	119443.5
34	163334.0
35	229434.0
36	325281.0
37	443195.0
38	582609.5
39	759051.0
40	970249.0
41	1173775.5
42	1356476.0
43	1521606.0
44	1641418.0
45	1700890.5
46	1707229.0
47	1657767.5
48	1557972.0
49	1418116.5
50	1235959.5
51	1036950.0
52	844740.0
53	671383.5
54	523489.5
55	394504.0
56	290752.5
57	214560.0
58	156172.5
59	112356.5
60	77988.0
61	51579.5
62	34462.5
63	23783.0
64	16385.5
65	11090.0
66	7747.0
67	5702.5
68	5080.5
69	3775.5
70	1819.5
71	1108.5
72	606.0
73	435.0
74	350.0
75	208.0
76	135.5
77	53.5
78	19.5
79	3.0
80	1.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.11626343029222382
9	0.0
10-14	8.569575461946178E-7
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	1.7139150923892357E-6
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	8.569575461946178E-7
95-99	8.569575461946178E-7
100-104	0.0
105-109	0.0
110-114	0.01900474750195804
115-119	0.0
120-124	8.569575461946178E-7
125-129	8.569575461946178E-7
130-134	0.04705896669173125
135-139	8.569575461946178E-7
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.3338379E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.548605353272805
#Duplication Level	Percentage of total
1	29.10863696352314
2	10.743655994301063
3	7.226082060307551
4	5.045957135101401
5	3.9295880013439684
6	3.1156576713515864
7	2.5528515487922316
8	2.067779187522157
9	1.8538753215008485
>10	20.989252174425683
>50	5.16647889552321
>100	6.20236439495687
>500	1.0437183959881815
>1k	0.8443211277515964
>5k	0.10978112761034976
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.2847877309730894E-6	8.569575461946179E-6	0.0	0.0	5.570224050265016E-4	0.0
2	8.569575461946179E-6	2.5708726385838535E-5	0.0	4.2847877309730894E-6	0.004344774759206712	0.0
3	4.7132665040703986E-5	2.5708726385838535E-5	0.0	4.2847877309730894E-6	0.0057244764085800474	0.0
4	9.855011781238106E-5	2.5708726385838535E-5	0.0	4.2847877309730894E-6	0.006967064850562243	0.0
5	9.855011781238106E-5	2.5708726385838535E-5	0.0	4.2847877309730894E-6	0.00832105777354974	0.0
6	1.0711969327432723E-4	2.5708726385838535E-5	0.0	8.569575461946179E-6	0.009675050696537235	0.0
7	1.1140448100530032E-4	2.9993514116811625E-5	4.2847877309730894E-6	8.569575461946179E-6	0.011397535364388417	0.0
8	1.4568278285308504E-4	3.4278301847784716E-5	4.2847877309730894E-6	8.569575461946179E-6	0.016265054226773847	0.0
9	1.585371460460043E-4	3.4278301847784716E-5	4.2847877309730894E-6	8.569575461946179E-6	0.021501064834022962	0.0
10-11	1.9924262949024866E-4	3.8563089578757806E-5	4.2847877309730894E-6	8.569575461946179E-6	0.027456919780075556	0.0
12-13	2.2280896201060064E-4	3.8563089578757806E-5	4.2847877309730894E-6	8.569575461946179E-6	0.032885745835218465	0.0
14-15	2.677992331858181E-4	4.713266504070398E-5	4.2847877309730894E-6	8.569575461946179E-6	0.0402041632797205	0.0
16-17	2.9350795957165665E-4	5.141745277167707E-5	4.2847877309730894E-6	8.569575461946179E-6	0.04543374670537316	0.0
18-19	3.513525939397934E-4	6.21294220991098E-5	4.2847877309730894E-6	8.569575461946179E-6	0.052525070400133614	0.0
20-21	3.9420047124952425E-4	7.069899756105598E-5	4.2847877309730894E-6	8.569575461946179E-6	0.05768823961595619	0.0
22-23	4.2419398536633584E-4	8.998054235043488E-5	4.2847877309730894E-6	8.569575461946179E-6	0.06315562876067785	0.0
24-25	4.906081951964187E-4	9.640772394689452E-5	4.2847877309730894E-6	8.569575461946179E-6	0.0677360668450881	0.0
26-27	5.677343743539344E-4	1.0283490554335414E-4	4.2847877309730894E-6	8.569575461946179E-6	0.07100321748995507	0.0
28-29	6.748540676282616E-4	1.0926208713981378E-4	4.2847877309730894E-6	8.569575461946179E-6	0.074358206283307	0.0
30-31	8.312488198087794E-4	1.1568926873627341E-4	4.2847877309730894E-6	1.2854363192919268E-5	0.07863228204495265	0.0
32-33	9.619348456034586E-4	1.1783166260175996E-4	4.2847877309730894E-6	1.2854363192919268E-5	0.08299419595508326	0.0
34-35	0.001073339326608759	1.2211645033273304E-4	4.2847877309730894E-6	1.2854363192919268E-5	0.08572146334584763	0.0
36-37	0.001133326354842382	1.2640123806370614E-4	4.2847877309730894E-6	1.2854363192919268E-5	0.08879151375508984	0.0
38-39	0.0011933133830760055	1.3282841966016576E-4	4.2847877309730894E-6	1.2854363192919268E-5	0.09188084570912144	0.0
40-41	0.0012340188665202498	1.3711320739113886E-4	8.569575461946179E-6	1.4996757058405813E-5	0.09434459865443097	0.0
42-43	0.0012447308358476824	1.5210996444954469E-4	8.569575461946179E-6	1.7139150923892358E-5	0.09694332241326614	0.0
44-45	0.0012811515315609538	1.7353390310441012E-4	8.569575461946179E-6	1.7139150923892358E-5	0.09899573573640225	0.0
46-47	0.0013197146211397115	2.1423938654865446E-4	8.569575461946179E-6	1.7139150923892358E-5	0.10135236898843747	0.0
48-49	0.0013797016493733347	2.206665681451141E-4	1.2854363192919268E-5	1.7139150923892358E-5	0.10341763667476649	0.0
50-51	0.0014246919205485524	2.2709374974157375E-4	1.2854363192919268E-5	1.7139150923892358E-5	0.1052943737009327	0.0
52-53	0.0014675397978582833	2.2709374974157375E-4	1.2854363192919268E-5	1.7139150923892358E-5	0.10715182918230953	0.0
54-55	0.0014868213426476621	2.506600822619258E-4	1.7139150923892358E-5	1.7139150923892358E-5	0.10887431385016072	2.1423938654865447E-6
56-57	0.0015189572506299602	2.5922965772387193E-4	1.7139150923892358E-5	1.7139150923892358E-5	0.11065035836464907	4.2847877309730894E-6
58-59	0.00158108667272907	2.63514445454845E-4	1.7139150923892358E-5	1.7139150923892358E-5	0.11237070063863476	4.2847877309730894E-6
60-61	0.0016603552457520722	2.763688086477643E-4	2.1423938654865448E-5	1.7139150923892358E-5	0.11380181974077977	8.569575461946179E-6
62-63	0.0016924911537343702	2.87080777975197E-4	2.1423938654865448E-5	1.7139150923892358E-5	0.11554144355955484	8.569575461946179E-6
64-65	0.001718199880120209	3.2350147368846824E-4	2.1423938654865448E-5	1.7139150923892358E-5	0.11722750753169275	8.569575461946179E-6
66-67	0.001801753240874184	3.492102000743068E-4	2.1423938654865448E-5	1.7139150923892358E-5	0.11837583064359354	8.569575461946179E-6
68-69	0.0018381739365874554	3.642069571327126E-4	2.1423938654865448E-5	1.7139150923892358E-5	0.11957342881440053	8.569575461946179E-6
70-71	0.0018467435120494014	3.642069571327126E-4	2.1423938654865448E-5	1.9281544789378903E-5	0.12073460628949423	8.569575461946179E-6
72-73	0.0018681674507042669	3.792037141911184E-4	2.1423938654865448E-5	2.1423938654865448E-5	0.12161727256207469	8.569575461946179E-6
74-75	0.0018895913893591325	3.877732896530646E-4	2.1423938654865448E-5	2.1423938654865448E-5	0.12259634655860203	8.569575461946179E-6
76-77	0.0019238696912069171	4.1133962217341656E-4	2.785112025132508E-5	2.1423938654865448E-5	0.1237810903662161	8.569575461946179E-6
78-79	0.0019881415071715137	4.156244099043897E-4	2.9993514116811625E-5	2.1423938654865448E-5	0.1253107595861735	8.569575461946179E-6
80-81	0.0019924262949024864	4.2419398536633584E-4	2.9993514116811625E-5	2.1423938654865448E-5	0.1266176198441203	8.569575461946179E-6
82-83	0.0019945686887679727	4.3276356082828205E-4	2.9993514116811625E-5	2.1423938654865448E-5	0.12805945091559273	8.569575461946179E-6
84-85	0.0020074230519608926	4.3704834855925513E-4	2.9993514116811625E-5	2.1423938654865448E-5	0.12950128198706518	1.2854363192919268E-5
86-87	0.0020202774151538115	4.499027117521744E-4	2.9993514116811625E-5	2.1423938654865448E-5	0.13107808387206327	1.2854363192919268E-5
88-89	0.002037416566077704	4.5847228721412057E-4	2.9993514116811625E-5	2.1423938654865448E-5	0.13267202490798524	1.2854363192919268E-5
90-91	0.0020438437476741635	4.670418626760668E-4	2.9993514116811625E-5	2.1423938654865448E-5	0.13366823805543648	1.4996757058405813E-5
92-93	0.002050270929270623	4.670418626760668E-4	3.213590798229817E-5	2.1423938654865448E-5	0.13452519560163112	1.7139150923892358E-5
94-95	0.002065267686329029	4.79896225868986E-4	3.4278301847784716E-5	2.5708726385838535E-5	0.13549141523496555	2.1423938654865448E-5
96-97	0.0020695524740600023	4.906081951964187E-4	3.4278301847784716E-5	2.5708726385838535E-5	0.13638265108300796	2.1423938654865448E-5
98-99	0.002099545988176814	5.013201645238514E-4	3.4278301847784716E-5	2.5708726385838535E-5	0.13728245650651227	2.1423938654865448E-5
100-101	0.002103830775907787	5.013201645238514E-4	3.4278301847784716E-5	2.5708726385838535E-5	0.13811799011405207	2.5708726385838535E-5
102-103	0.00210811556363876	5.013201645238514E-4	3.4278301847784716E-5	2.5708726385838535E-5	0.1390156531436909	2.5708726385838535E-5
104-105	0.002112400351369733	5.056049522548246E-4	3.4278301847784716E-5	2.5708726385838535E-5	0.1398918922346749	2.5708726385838535E-5
106-107	0.0021488210470830046	5.184593154477439E-4	3.4278301847784716E-5	2.5708726385838535E-5	0.1405774582716306	2.5708726385838535E-5
108-109	0.002155248228679464	5.31313678640663E-4	3.8563089578757806E-5	2.5708726385838535E-5	0.14132086894295443	2.5708726385838535E-5
110-111	0.0021681025918723834	5.398832541026093E-4	4.2847877309730896E-5	2.5708726385838535E-5	0.14220782000326587	2.5708726385838535E-5
112-113	0.0021766721673343293	5.441680418335823E-4	4.2847877309730896E-5	2.5708726385838535E-5	0.14297908179484103	2.5708726385838535E-5
114-115	0.0021809569550653024	5.50595223430042E-4	4.2847877309730896E-5	2.5708726385838535E-5	0.1437974762514569	2.5708726385838535E-5
116-117	0.0021873841366617623	5.527376172955286E-4	4.2847877309730896E-5	2.5708726385838535E-5	0.1446887120994993	2.5708726385838535E-5
118-119	0.0022002384998546813	5.527376172955286E-4	4.2847877309730896E-5	2.5708726385838535E-5	0.1453721357425895	2.9993514116811625E-5
120-121	0.0022130928630476007	5.570224050265016E-4	4.2847877309730896E-5	2.5708726385838535E-5	0.14623766286424605	2.9993514116811625E-5
122-123	0.0022216624385095465	5.613071927574747E-4	4.2847877309730896E-5	2.5708726385838535E-5	0.1468503875097752	2.9993514116811625E-5
124-125	0.0022366591955679527	5.720191620849075E-4	4.7132665040703986E-5	2.5708726385838535E-5	0.14767735154185302	2.9993514116811625E-5
126-127	0.002258083134222818	5.805887375468536E-4	4.7132665040703986E-5	2.5708726385838535E-5	0.14859643851014676	3.4278301847784716E-5
128-129	0.0022752222851467104	5.891583130087999E-4	4.7132665040703986E-5	2.5708726385838535E-5	0.14934627636306702	4.2847877309730896E-5
130-131	0.00228807664833963	6.105822516636653E-4	4.7132665040703986E-5	2.5708726385838535E-5	0.15016681321354836	4.2847877309730896E-5
132-133	0.002315927768590955	6.127246455291518E-4	4.7132665040703986E-5	2.5708726385838535E-5	0.15101948597201204	4.499027117521744E-5
134-135	0.00234377888884228	6.148670393946383E-4	4.7132665040703986E-5	2.5708726385838535E-5	0.1517843205819907	5.141745277167707E-5
136-137	0.0023502060704387396	6.170094332601249E-4	4.7132665040703986E-5	2.5708726385838535E-5	0.15261985418953047	5.570224050265016E-5
138-139	0.002360918039766172	6.234366148565845E-4	4.7132665040703986E-5	2.5708726385838535E-5	0.1533804040117782	5.998702823362325E-5
>>END_MODULE
