##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.2d.S.1.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24190034
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.893661331769934	37.0	35.0	38.0	33.0	39.0
2	35.48516099646656	35.0	34.0	38.0	33.0	39.0
3	35.59349118732119	35.0	35.0	38.0	33.0	39.0
4	35.67536593788996	36.0	35.0	38.0	33.0	39.0
5	35.6029728606417	36.0	35.0	38.0	33.0	39.0
6	35.54399005805448	36.0	35.0	37.0	33.0	39.0
7	35.57149394663935	35.0	34.0	38.0	33.0	39.0
8	35.68600676625754	36.0	35.0	38.0	33.0	39.0
9	35.67055205461886	36.0	35.0	38.0	33.0	39.0
10-14	35.64354782634865	35.8	35.0	37.8	33.0	39.0
15-19	35.62047821842664	36.0	35.0	38.0	33.0	39.0
20-24	35.60977931655656	36.0	35.0	38.0	33.0	39.0
25-29	35.59306980717762	36.0	35.0	38.0	33.0	39.0
30-34	35.57856395737186	36.0	35.0	38.0	33.0	39.0
35-39	35.54944126990479	36.0	35.0	38.0	33.0	39.0
40-44	35.536643867470374	36.0	35.0	38.0	33.0	39.0
45-49	35.50662441400455	36.0	35.0	38.0	33.0	39.0
50-54	35.4887301853317	36.0	35.0	38.0	33.0	39.0
55-59	35.46241765513848	36.0	34.8	38.0	33.0	39.0
60-64	35.42192401217791	36.0	34.0	38.0	33.0	39.0
65-69	35.39440422448352	36.0	34.0	38.0	32.8	39.0
70-74	35.36267506693046	36.0	34.0	38.0	32.0	39.0
75-79	35.311701165860285	36.0	34.0	38.0	32.0	39.0
80-84	35.27229459867646	36.0	34.0	38.0	32.0	39.0
85-89	35.22238504501482	36.0	34.0	38.0	32.0	39.0
90-94	35.171383967463626	36.0	34.0	38.0	32.0	39.0
95-99	35.10256366733507	36.0	34.0	38.0	32.0	39.0
100-104	35.056103955868764	36.0	34.0	38.0	32.0	39.0
105-109	34.99872063015703	36.0	34.0	38.0	31.2	39.0
110-114	34.92120120211489	35.2	34.0	38.0	31.0	39.0
115-119	34.84580516091875	35.0	34.0	37.6	31.0	39.0
120-124	34.77150390115202	35.0	34.0	37.0	31.0	39.0
125-129	34.692903540358806	35.0	34.0	37.0	30.0	39.0
130-134	34.60305382786977	35.0	34.0	37.0	30.0	39.0
135-139	34.526260765073744	35.0	34.0	37.0	30.0	39.0
140-144	34.39532810082036	35.0	34.0	37.0	29.8	39.0
145-149	34.31962206419387	35.0	34.0	37.0	29.2	39.0
150	34.261821996612326	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	12.0
21	215.0
22	1678.0
23	8615.0
24	27937.0
25	66751.0
26	130944.0
27	227256.0
28	338743.0
29	450878.0
30	582435.0
31	763983.0
32	1030939.0
33	1450682.0
34	2201424.0
35	3793865.0
36	1.0596998E7
37	2516670.0
38	9.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.76013444214258	18.779597416026782	15.395054839526063	32.06521330230458
2	24.649093093461545	25.501084454862692	31.963158877742792	17.886663573932967
3	20.21589552127128	29.293294916410616	29.683443189869017	20.807366372449085
4	23.657395438137872	33.93729831053565	21.49902311009567	20.906283141230805
5	25.768512768522772	34.920748767860346	21.44279747601843	17.867940987598445
6	19.262056432000055	39.25953969308187	21.51319836921271	19.965205505705367
7	20.687217719495557	20.773385436332994	36.73971686025741	21.799679983914036
8	21.749700723860084	23.474762375282314	27.90822451923796	26.867312381619634
9	23.830847033947947	23.278508000443487	27.468774950874398	25.421870014734164
10-14	23.20327371181041	28.856796976804578	25.07265843446107	22.867270876923943
15-19	23.343129654137734	27.647693260786653	26.589662503161428	22.41951458191419
20-24	23.17984670877271	27.65608101253599	26.52993708070026	22.63413519799104
25-29	23.277954880096487	27.22763018853136	26.536321528113604	22.958093403258548
30-34	23.08441319263958	26.941244481094984	26.785365411226792	23.188976915038648
35-39	23.43011754344785	26.6706222901547	26.793815998770405	23.10544416762705
40-44	23.618817567598292	26.327041954550374	26.83631531894498	23.21782515890635
45-49	23.49182063985524	26.144487436437668	26.950813711134096	23.412878212572995
50-54	23.69637016632552	26.167846642960484	26.656175018191377	23.479608172522617
55-59	23.757998025137127	26.17122324011616	26.709182798172172	23.361595936574542
60-64	23.40592080193025	26.141481239753528	26.804036736781768	23.648561221534457
65-69	23.620677837823624	26.061802145462053	26.80981762985534	23.507702386858988
70-74	23.78530183132442	26.037891472165768	26.51609584343701	23.660710853072796
75-79	23.622289245232146	26.15752503696357	26.77776228011916	23.44242343768512
80-84	23.966268290208014	26.158878703178996	26.43695933984185	23.43789366677115
85-89	23.662126312017588	26.275844010802135	26.68561854853119	23.376411128649096
90-94	23.697530148159363	26.014323915377712	26.690971992846315	23.597173943616614
95-99	23.683800527109636	26.20612273633018	26.758575866408457	23.351500870151735
100-104	23.95322635759834	25.953317800214748	26.702262592933934	23.39119324925298
105-109	23.74769791559615	26.179436539857697	26.583903106543794	23.48896243800236
110-114	23.85410371891168	26.29844009313918	26.48582717990392	23.361629008045213
115-119	23.97351653164274	26.355713266049978	26.66429241066796	23.00647779163932
120-124	23.944752620025255	26.52008839673396	26.781639083268754	22.75351989997203
125-129	23.913837175603074	26.592917395475773	26.60432870671439	22.88891672220676
130-134	24.10524480020735	26.618857590461154	26.57422333615304	22.701674273178455
135-139	23.870418899538702	26.62458784989379	26.859991406874347	22.645001843693166
140-144	23.889121445633354	26.826097888080685	26.60594937568091	22.678831290605046
145-149	24.07201329274692	27.619692473354934	25.924817633575874	22.383476600322265
150	23.832062410495165	27.51681539596017	25.847565985231768	22.803556208312894
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	0.5
9	2.5
10	2.5
11	1.0
12	7.0
13	10.5
14	14.5
15	22.5
16	40.5
17	83.5
18	152.5
19	245.5
20	405.5
21	620.5
22	950.0
23	1549.0
24	2484.5
25	3996.5
26	6420.0
27	9999.0
28	15040.5
29	21698.0
30	31262.5
31	45984.5
32	67327.5
33	93113.0
34	126028.0
35	180669.0
36	258374.0
37	348393.0
38	458200.5
39	598835.5
40	769903.5
41	955998.5
42	1149509.0
43	1334546.0
44	1482494.5
45	1586174.5
46	1622867.5
47	1594016.0
48	1551747.5
49	1509483.5
50	1406987.0
51	1248356.0
52	1075980.0
53	908862.5
54	765392.0
55	650634.5
56	543533.0
57	445461.0
58	371687.5
59	307655.0
60	237066.0
61	162139.0
62	100276.0
63	60523.5
64	37006.5
65	18148.5
66	9583.0
67	5047.0
68	2422.0
69	1391.5
70	938.5
71	742.0
72	458.5
73	368.0
74	317.5
75	196.5
76	123.0
77	47.5
78	14.0
79	2.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	8.267867668147965E-7
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	2.4803603004443895E-6
130-134	0.002234804630700395
135-139	8.267867668147965E-7
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4190034E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.59077651441146
#Duplication Level	Percentage of total
1	25.403449259208582
2	8.20395933119675
3	5.013200269244619
4	3.5796613270662525
5	2.6712766967658244
6	2.1764282068960012
7	1.799997731284721
8	1.5013708109125006
9	1.2528363902765036
>10	16.457615488801412
>50	5.874279567404474
>100	13.228244936216576
>500	4.623155993320303
>1k	6.450168380180646
>5k	1.1273063719537686
>10k+	0.6370492392709666
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	32969	0.13629166457558514	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.596522435644365E-4	4.1339338340739824E-6	0.0	0.0	0.0022075206673955066	0.0
2	0.001955350703516994	1.653573533629593E-5	0.0	0.0	0.010500191938547915	0.0
3	0.0029598966251969715	2.0669669170369915E-5	0.0	0.0	0.014940036876343373	0.0
4	0.007147571599113916	2.0669669170369915E-5	0.0	0.0	0.022149617482968398	0.0
5	0.007362536158485763	2.0669669170369915E-5	0.0	0.0	0.026936712862826073	0.0
6	0.00857377877186944	2.0669669170369915E-5	0.0	0.0	0.035266589538485146	0.0
7	0.0108681120497805	2.4803603004443896E-5	0.0	0.0	0.04806938262261227	4.1339338340739824E-6
8	0.019053301041246987	2.4803603004443896E-5	0.0	0.0	0.0659569143226504	4.1339338340739824E-6
9	0.019681658984026233	2.4803603004443896E-5	0.0	0.0	0.07870596626693456	4.1339338340739824E-6
10-11	0.020477441247085473	2.4803603004443896E-5	0.0	0.0	0.1315624442694045	4.1339338340739824E-6
12-13	0.02138690669058175	2.8937536838517878E-5	0.0	0.0	0.1573437226256069	4.1339338340739824E-6
14-15	0.0249379558540513	2.8937536838517878E-5	0.0	0.0	0.16916057249030736	4.1339338340739824E-6
16-17	0.029164903199391946	2.8937536838517878E-5	0.0	0.0	0.17715146659157238	4.1339338340739824E-6
18-19	0.037513382577304355	3.1004503755554865E-5	0.0	0.0	0.18480957901919443	4.1339338340739824E-6
20-21	0.04452453435989383	3.307147067259186E-5	0.0	0.0	0.18987984886668619	4.1339338340739824E-6
22-23	0.04741622107682858	3.720540450666584E-5	0.0	4.1339338340739824E-6	0.19513407876979422	4.1339338340739824E-6
24-25	0.053532376184341035	3.9272371423702835E-5	0.0	4.1339338340739824E-6	0.20036763900373186	4.1339338340739824E-6
26-27	0.05878867305436611	4.547327217481381E-5	0.0	4.1339338340739824E-6	0.20445609956563104	4.1339338340739824E-6
28-29	0.07132069347236139	5.167417292592478E-5	0.0	4.1339338340739824E-6	0.208085693471948	4.1339338340739824E-6
30-31	0.09607055533696232	5.3741139842961774E-5	0.0	4.1339338340739824E-6	0.21214108256317457	4.1339338340739824E-6
32-33	0.11675676024266853	5.7875073677035756E-5	0.0	4.1339338340739824E-6	0.2155949842815434	4.1339338340739824E-6
34-35	0.13220320401368596	5.7875073677035756E-5	0.0	4.1339338340739824E-6	0.21904888599991218	4.1339338340739824E-6
36-37	0.13485718953516146	5.7875073677035756E-5	0.0	4.1339338340739824E-6	0.22106417874402326	4.1339338340739824E-6
38-39	0.1370998486401466	5.7875073677035756E-5	0.0	4.1339338340739824E-6	0.2227983639874173	4.1339338340739824E-6
40-41	0.13856739515124286	5.994204059407275E-5	0.0	4.1339338340739824E-6	0.22446640628946615	6.200900751110974E-6
42-43	0.13885056961887693	6.407597442814673E-5	0.0	4.1339338340739824E-6	0.22594015370131354	8.267867668147965E-6
44-45	0.13969595908794508	6.82099082622207E-5	0.0	4.1339338340739824E-6	0.2271451954139461	8.267867668147965E-6
46-47	0.14116350559904134	8.061170976444266E-5	0.0	4.1339338340739824E-6	0.2285776034874527	8.267867668147965E-6
48-49	0.14246156082294054	8.267867668147966E-5	4.1339338340739824E-6	4.1339338340739824E-6	0.22975370766324676	8.267867668147965E-6
50-51	0.14336895929951982	8.681261051555363E-5	4.1339338340739824E-6	4.1339338340739824E-6	0.23094841454129417	8.267867668147965E-6
52-53	0.1441792103309983	9.301351126666461E-5	4.1339338340739824E-6	4.1339338340739824E-6	0.2320955811802497	1.0334834585184957E-5
54-55	0.14461740731741013	9.921441201777559E-5	4.1339338340739824E-6	4.1339338340739824E-6	0.23318693971244522	1.2401801502221948E-5
56-57	0.14538425204363087	9.921441201777559E-5	4.1339338340739824E-6	4.1339338340739824E-6	0.2340447309830156	1.2401801502221948E-5
58-59	0.14553514062857456	1.0334834585184957E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.2348384462791578	1.2401801502221948E-5
60-61	0.14577077485711679	1.0748227968592356E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.23560529100537847	1.2401801502221948E-5
62-63	0.1459836724495716	1.198840811881455E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.23656436365488365	1.2401801502221948E-5
64-65	0.14601467695332715	1.2195104810518249E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.23735601198410883	1.2401801502221948E-5
66-67	0.14609528866309157	1.2815194885629346E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.23796576722463475	1.2401801502221948E-5
68-69	0.14613662800143234	1.2815194885629346E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.2387801521899473	1.2401801502221948E-5
70-71	0.14626271298337157	1.3435284960740444E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.23946225127256954	1.2401801502221948E-5
72-73	0.14632265502396566	1.4882161802666336E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.23995625636574136	1.653573533629593E-5
74-75	0.14634332469313605	1.5915645261184834E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.24050813653259023	1.653573533629593E-5
76-77	0.14637226222997454	1.694912871970333E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.241221240118968	2.0669669170369915E-5
78-79	0.14640533370064712	1.8189308869925524E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.2422154512060628	2.4803603004443896E-5
80-81	0.14643220427056863	1.922279232844402E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.2429657601969472	3.1004503755554865E-5
82-83	0.14646734270815825	1.9842882403555117E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.24368919861791016	3.720540450666584E-5
84-85	0.14650661507958196	2.1083062553777312E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.24446637817871608	3.720540450666584E-5
86-87	0.1465252177818353	2.149645593718471E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.24523735683877088	3.720540450666584E-5
88-89	0.1465314186825864	2.149645593718471E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.24596699616048495	3.720540450666584E-5
90-91	0.1465376195833375	2.3150029470814302E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.24652507722808492	3.720540450666584E-5
92-93	0.14654795441792268	2.3150029470814302E-4	4.1339338340739824E-6	4.1339338340739824E-6	0.24703975199042713	3.720540450666584E-5
94-95	0.14660582949159973	2.3563422854221703E-4	8.267867668147965E-6	4.1339338340739824E-6	0.24774045377530268	3.720540450666584E-5
96-97	0.14661409735926786	2.3563422854221703E-4	1.0334834585184957E-5	4.1339338340739824E-6	0.24840601712258858	4.133933834073983E-5
98-99	0.14662443219385307	2.3563422854221703E-4	1.2401801502221948E-5	4.1339338340739824E-6	0.24897856695860782	4.340630525777682E-5
100-101	0.14663890096227233	2.3563422854221703E-4	1.2401801502221948E-5	1.2401801502221948E-5	0.2495180453239545	4.960720600888779E-5
102-103	0.1466533697306916	2.3563422854221703E-4	1.4468768419258939E-5	1.2401801502221948E-5	0.2500285861524626	4.960720600888779E-5
104-105	0.14669264210211527	2.3563422854221703E-4	1.653573533629593E-5	1.2401801502221948E-5	0.25053292608021965	4.960720600888779E-5
106-107	0.14671951267203676	2.4183512929332797E-4	1.653573533629593E-5	1.2401801502221948E-5	0.25103519904105964	5.167417292592478E-5
108-109	0.14673398144045602	2.48036030044439E-4	1.860270225333292E-5	1.2401801502221948E-5	0.25147752996130557	5.3741139842961774E-5
110-111	0.14678565561338192	2.521699638785129E-4	2.0669669170369915E-5	1.2401801502221948E-5	0.2520459458634907	5.3741139842961774E-5
112-113	0.14679805741488416	2.5630389771258693E-4	2.0669669170369915E-5	1.2401801502221948E-5	0.25257715636116923	5.7875073677035756E-5
114-115	0.14680425831563526	2.5630389771258693E-4	2.0669669170369915E-5	1.2401801502221948E-5	0.25305255875208776	5.7875073677035756E-5
116-117	0.14681045921638639	2.5630389771258693E-4	2.2736636087406906E-5	1.2401801502221948E-5	0.2535837692497662	5.7875073677035756E-5
118-119	0.1468228610178886	2.5630389771258693E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.25409637704519145	5.994204059407275E-5
120-121	0.14683732978630787	2.5837086462962393E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.2547081992526344	6.407597442814673E-5
122-123	0.1468600664223953	2.6250479846369793E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.2552022043458062	6.614294134518372E-5
124-125	0.1468848700253997	2.645717653807349E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.25580782565249804	6.614294134518372E-5
126-127	0.1469117405953212	2.645717653807349E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.25634937098476174	6.614294134518372E-5
128-129	0.14693034329757454	2.645717653807349E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.2569529253245365	6.614294134518372E-5
130-131	0.14693654419832564	2.645717653807349E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.2576391583409928	6.82099082622207E-5
132-133	0.14695307993366194	2.645717653807349E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.25828198505219135	7.027687517925771E-5
134-135	0.14695928083441306	2.666387322977719E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.2588049276822017	7.027687517925771E-5
136-137	0.14696961566899824	2.687056992148089E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.2593526739152165	7.027687517925771E-5
138-139	0.14700062017275378	2.7697356688295683E-4	2.4803603004443896E-5	1.2401801502221948E-5	0.25996036218882534	7.441080901333168E-5
>>END_MODULE
