##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.2d.S.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24075142
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.76541774914557	37.0	35.0	38.0	33.0	39.0
2	35.386248189107256	35.0	34.0	37.0	33.0	39.0
3	35.51835877022034	35.0	34.0	37.0	33.0	39.0
4	35.58666669546539	36.0	35.0	38.0	33.0	39.0
5	35.50437193683011	36.0	35.0	38.0	33.0	39.0
6	35.4506883490033	35.0	35.0	37.0	33.0	39.0
7	35.48291615476245	35.0	34.0	38.0	33.0	39.0
8	35.59359724648768	36.0	35.0	38.0	33.0	39.0
9	35.57435843992114	36.0	35.0	38.0	33.0	39.0
10-14	35.55260209887858	35.8	35.0	37.8	33.0	39.0
15-19	35.53084916383879	36.0	35.0	38.0	33.0	39.0
20-24	35.521322275066964	36.0	35.0	38.0	33.0	39.0
25-29	35.50576925361437	36.0	35.0	38.0	33.0	39.0
30-34	35.48962417750225	36.0	35.0	38.0	33.0	39.0
35-39	35.462846790270234	36.0	34.4	38.0	33.0	39.0
40-44	35.448428939692235	36.0	34.0	38.0	33.0	39.0
45-49	35.41969810188451	36.0	34.0	38.0	33.0	39.0
50-54	35.39991542313645	36.0	34.0	38.0	33.0	39.0
55-59	35.37524412524753	36.0	34.0	38.0	32.2	39.0
60-64	35.334772820862284	36.0	34.0	38.0	32.0	39.0
65-69	35.30856302322122	36.0	34.0	38.0	32.0	39.0
70-74	35.27873531960891	36.0	34.0	38.0	32.0	39.0
75-79	35.22933934096837	36.0	34.0	38.0	32.0	39.0
80-84	35.18834268142634	36.0	34.0	38.0	32.0	39.0
85-89	35.14031146316812	36.0	34.0	38.0	32.0	39.0
90-94	35.090161702888395	36.0	34.0	38.0	32.0	39.0
95-99	35.02463019325078	35.2	34.0	37.8	32.0	39.0
100-104	34.979306797027405	35.2	34.0	38.0	31.0	39.0
105-109	34.92167752115439	35.0	34.0	37.2	31.0	39.0
110-114	34.84706788437634	35.0	34.0	37.0	31.0	39.0
115-119	34.77552964796635	35.0	34.0	37.0	31.0	39.0
120-124	34.7046883046422	35.0	34.0	37.0	30.6	39.0
125-129	34.62680853138893	35.0	34.0	37.0	30.0	39.0
130-134	34.52369850196523	35.0	34.0	37.0	30.0	39.0
135-139	34.4668302434104	35.0	34.0	37.0	30.0	39.0
140-144	34.344734274049145	35.0	34.0	37.0	29.6	39.0
145-149	34.266754239705	35.0	34.0	37.0	29.0	39.0
150	34.20039213060509	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	13.0
21	200.0
22	1808.0
23	9137.0
24	29650.0
25	71346.0
26	140035.0
27	237512.0
28	349446.0
29	463417.0
30	605586.0
31	799224.0
32	1080261.0
33	1522055.0
34	2296908.0
35	3934518.0
36	1.0265255E7
37	2268767.0
38	4.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.511600554630164	18.672541993729467	15.631882877367866	33.183974574272504
2	23.641227121318746	25.406599886305965	32.96370173019125	17.988471262184042
3	20.055250349094514	29.052190014081745	29.76764166126206	21.12491797556168
4	23.45275471272402	34.00006114190313	21.31389713090789	21.23328701446496
5	25.69016623037987	34.79206062419071	21.57313547724869	17.944637668180732
6	19.300891350921212	38.79819275832308	21.870230298122436	20.030685592633265
7	20.48817406767528	20.294920794236646	37.12657229602218	22.090332842065898
8	21.72739832645639	23.001559035456573	28.05245343931928	27.218589198767756
9	23.650236413974216	22.999170679865564	27.69564142134655	25.654951484813672
10-14	23.09895576109167	28.463529727052077	25.24291985484447	23.194594657011784
15-19	23.201363464439794	27.57342822733922	26.608367252828664	22.61684105539232
20-24	23.039470338326563	27.654550074927908	26.47853125850722	22.827448328238315
25-29	23.163393179570864	27.270345487474174	26.433351878049148	23.13290945490581
30-34	23.0129749598154	26.97508658515908	26.641191150606712	23.370747304418806
35-39	23.359514971915846	26.737426512375293	26.649217686857256	23.2538408288516
40-44	23.52114578199494	26.395670076593277	26.665431312225955	23.41775282918583
45-49	23.42503566541788	26.22683762363686	26.766634232105464	23.58149247883979
50-54	23.6143869888701	26.221038280895705	26.51236283466158	23.65221189557262
55-59	23.638351125820982	26.199816391529485	26.56793966158123	23.593892821068305
60-64	23.294239344465755	26.12815243208119	26.7051592052915	23.872449018161554
65-69	23.501684214379164	26.04244765029795	26.715950640839008	23.739917494483876
70-74	23.66001994920736	26.00795708702362	26.456385594735014	23.875637369034003
75-79	23.52947118650432	26.072913713239988	26.70640862679024	23.691206473465453
80-84	23.847254566556657	26.0703425965255	26.41279042092462	23.669612415993228
85-89	23.530232137363924	26.21989602387392	26.62655863047454	23.62331320828762
90-94	23.554287654876553	25.93330332174157	26.647490594240313	23.86491842914156
95-99	23.564060390588764	26.101098801411016	26.739474267690717	23.595366540309502
100-104	23.830543554011022	25.867632265678846	26.650375727794252	23.65144845251588
105-109	23.642877786556774	26.119983840593754	26.51963838884107	23.717499984008402
110-114	23.71544475210157	26.197471234022213	26.470194859079125	23.616889154797093
115-119	23.858107254362196	26.308697161578525	26.618291181833943	23.214904402225333
120-124	23.79855889363858	26.491447705616423	26.72332837516247	22.98666502558253
125-129	23.819897916447577	26.520019084581257	26.54992794268817	23.11015505628299
130-134	23.989715599897963	26.544851754174037	26.531392964832406	22.934039681095594
135-139	23.7981923429569	26.57834873829612	26.799921678551264	22.82353724019572
140-144	23.82914792361349	26.73244959468983	26.57750637566333	22.86089610603335
145-149	23.956015711143053	27.365062270453066	26.049312606338937	22.62960941206494
150	23.774916883148602	27.33471727809539	25.98318630893226	22.907179529823747
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	1.0
10	1.5
11	3.0
12	5.0
13	10.5
14	19.0
15	26.0
16	46.0
17	89.5
18	162.0
19	258.0
20	403.0
21	630.0
22	961.5
23	1533.0
24	2469.0
25	4039.0
26	6422.0
27	10033.5
28	15338.0
29	21802.5
30	30912.5
31	44868.0
32	65412.5
33	90282.5
34	121299.0
35	174639.0
36	249210.0
37	335046.0
38	441795.0
39	580954.0
40	753886.5
41	937981.0
42	1127210.0
43	1311311.5
44	1458534.0
45	1566138.0
46	1604362.0
47	1576368.5
48	1543877.5
49	1512804.0
50	1414732.0
51	1258391.5
52	1090204.0
53	922548.5
54	774566.5
55	661566.5
56	557024.0
57	456999.5
58	381525.0
59	316033.5
60	243859.5
61	165370.0
62	101549.5
63	62220.5
64	38292.5
65	18260.5
66	9588.5
67	5028.5
68	2440.0
69	1284.5
70	731.5
71	576.5
72	366.0
73	276.5
74	246.0
75	152.5
76	100.5
77	38.5
78	16.0
79	3.5
80	2.0
81	1.5
82	1.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	8.30732379480877E-7
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	8.30732379480877E-7
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	8.30732379480877E-7
125-129	8.30732379480877E-7
130-134	0.05007820929986622
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4075142E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.46289284828154
#Duplication Level	Percentage of total
1	24.350155529726855
2	7.981810721837561
3	5.0947885550646905
4	3.5541018811533904
5	2.6858001193183876
6	2.1278212665548675
7	1.8234152505319678
8	1.4794959046696476
9	1.3387165051257173
>10	16.569691325189666
>50	6.0496158702146525
>100	13.61720928579247
>500	4.843931844920499
>1k	6.689165671557051
>5k	0.8691781286798669
>10k+	0.9251021396625829
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	28749	0.11941362588847866	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.201440805624324E-4	0.0	0.0	0.0	0.002039447991625553	0.0
2	0.0012211765978368892	1.661464758961754E-5	0.0	0.0	0.009769412782695113	0.0
3	0.001756998982602055	1.661464758961754E-5	0.0	0.0	0.014168140732046357	0.0
4	0.004569028087144824	1.661464758961754E-5	0.0	0.0	0.02060216301112575	0.0
5	0.00472686723924619	1.661464758961754E-5	0.0	0.0	0.02516703743637317	0.0
6	0.0056240582090855374	1.661464758961754E-5	0.0	0.0	0.03245256040442046	0.0
7	0.007480745077225298	2.9075633281830694E-5	0.0	0.0	0.04379621104623183	0.0
8	0.014267828617584062	2.9075633281830694E-5	0.0	0.0	0.059405672456677515	0.0
9	0.014919953535476552	2.9075633281830694E-5	0.0	0.0	0.0707576304222837	0.0
10-11	0.015611538241394381	3.322929517923508E-5	2.0768309487021926E-6	0.0	0.11608861953960645	0.0
12-13	0.016346736397234957	3.7382957076639467E-5	4.153661897404385E-6	0.0	0.13808433611731136	0.0
14-15	0.019046616630547807	3.7382957076639467E-5	4.153661897404385E-6	0.0	0.14820265649938846	0.0
16-17	0.02224285946060048	4.153661897404385E-5	4.153661897404385E-6	0.0	0.15566055643617804	0.0
18-19	0.029736065523517992	4.153661897404385E-5	4.153661897404385E-6	0.0	0.16354836037934894	0.0
20-21	0.036186702450187	4.153661897404385E-5	4.153661897404385E-6	0.0	0.16848914120631148	0.0
22-23	0.03910257310216488	4.5690280871448235E-5	4.153661897404385E-6	0.0	0.17358983801632405	0.0
24-25	0.04580866023552426	4.5690280871448235E-5	4.153661897404385E-6	0.0	0.17880060686661786	0.0
26-27	0.05163417104663391	4.5690280871448235E-5	4.153661897404385E-6	0.0	0.18295011510212483	0.0
28-29	0.06468289989732978	4.5690280871448235E-5	4.153661897404385E-6	0.0	0.1870850855209909	0.0
30-31	0.09148648012128027	4.984394276885262E-5	4.153661897404385E-6	0.0	0.1914256622037785	4.153661897404385E-6
32-33	0.11143444138356484	5.3997604666257004E-5	4.153661897404385E-6	0.0	0.19457206109106231	4.153661897404385E-6
34-35	0.12575834443676387	5.3997604666257004E-5	4.153661897404385E-6	0.0	0.19793237356606247	4.153661897404385E-6
36-37	0.12776040947131279	5.3997604666257004E-5	4.153661897404385E-6	0.0	0.20001128134571333	4.153661897404385E-6
38-39	0.12961501950850385	5.8151266563661396E-5	4.153661897404385E-6	0.0	0.2015792887119835	4.153661897404385E-6
40-41	0.1309171925133401	6.230492846106578E-5	8.30732379480877E-6	0.0	0.20320129368291992	4.153661897404385E-6
42-43	0.13118302687477398	6.438175940976797E-5	8.30732379480877E-6	0.0	0.2047921461896258	4.153661897404385E-6
44-45	0.13199091411381914	7.476591415327893E-5	8.30732379480877E-6	2.0768309487021926E-6	0.20608601187066727	4.153661897404385E-6
46-47	0.13346338725644902	7.891957605068332E-5	8.30732379480877E-6	4.153661897404385E-6	0.20737780072076	4.153661897404385E-6
48-49	0.1346866406852346	8.099640699938551E-5	8.30732379480877E-6	4.153661897404385E-6	0.20861559196618654	4.153661897404385E-6
50-51	0.13566275123112462	8.30732379480877E-5	8.30732379480877E-6	4.153661897404385E-6	0.2098180770854851	4.153661897404385E-6
52-53	0.1365994019889893	8.30732379480877E-5	8.30732379480877E-6	4.153661897404385E-6	0.21100810121909147	4.153661897404385E-6
54-55	0.1370749962762421	9.345739269159866E-5	8.30732379480877E-6	4.153661897404385E-6	0.21207559232672438	4.153661897404385E-6
56-57	0.13774788950362163	9.553422364030086E-5	8.30732379480877E-6	4.153661897404385E-6	0.21309531632253717	4.153661897404385E-6
58-59	0.13787457619149246	9.968788553770524E-5	8.30732379480877E-6	8.30732379480877E-6	0.21401742926376094	4.153661897404385E-6
60-61	0.13819233132664388	1.0384154743510962E-4	8.30732379480877E-6	8.30732379480877E-6	0.21472562861726838	4.153661897404385E-6
62-63	0.13846231934997516	1.0799520933251401E-4	8.30732379480877E-6	8.30732379480877E-6	0.21561866592521034	4.153661897404385E-6
64-65	0.13847685716661606	1.1214887122991839E-4	8.30732379480877E-6	8.30732379480877E-6	0.21654908619022892	4.153661897404385E-6
66-67	0.13855369991171806	1.2045619502472716E-4	8.30732379480877E-6	8.30732379480877E-6	0.21722405624855712	6.230492846106578E-6
68-69	0.13860562068543564	1.3291718071694033E-4	8.30732379480877E-6	8.30732379480877E-6	0.2179634080662951	8.30732379480877E-6
70-71	0.13871153906381944	1.349940116656425E-4	8.30732379480877E-6	8.30732379480877E-6	0.21868822206739216	8.30732379480877E-6
72-73	0.1387676134994344	1.370708426143447E-4	8.30732379480877E-6	8.30732379480877E-6	0.2191742005093885	1.0384154743510963E-5
74-75	0.1387883818089214	1.4537816640915348E-4	1.661464758961754E-5	8.30732379480877E-6	0.21977648148451212	1.2460985692213155E-5
76-77	0.13881122694935713	1.5160865925526005E-4	1.661464758961754E-5	1.2460985692213155E-5	0.22058852238545468	1.2460985692213155E-5
78-79	0.13888391603256173	1.5783915210136664E-4	1.661464758961754E-5	1.2460985692213155E-5	0.22167470497162592	1.2460985692213155E-5
80-81	0.13898152708715072	1.6199281399877102E-4	2.0768309487021926E-5	1.2460985692213155E-5	0.22243067143695353	1.4537816640915349E-5
82-83	0.13900852588948387	1.6199281399877102E-4	2.0768309487021926E-5	1.2460985692213155E-5	0.22321986719746034	1.661464758961754E-5
84-85	0.13906460032509882	1.6199281399877102E-4	2.0768309487021926E-5	1.2460985692213155E-5	0.2240256776055568	1.661464758961754E-5
86-87	0.13910198328217543	1.6199281399877102E-4	2.2845140435724118E-5	1.2460985692213155E-5	0.2248024123803714	1.661464758961754E-5
88-89	0.13914144307020077	1.661464758961754E-4	2.492197138442631E-5	1.2460985692213155E-5	0.22556876300044254	1.661464758961754E-5
90-91	0.13917674919632872	1.661464758961754E-4	2.492197138442631E-5	1.2460985692213155E-5	0.22613366101848953	2.0768309487021926E-5
92-93	0.13919751750581574	1.661464758961754E-4	2.9075633281830694E-5	1.2460985692213155E-5	0.22663625410807547	2.0768309487021926E-5
94-95	0.1392452846176359	1.72376968742282E-4	2.9075633281830694E-5	1.2460985692213155E-5	0.2272447655760452	2.0768309487021926E-5
96-97	0.1392473614485846	1.7445379969098417E-4	2.9075633281830694E-5	1.2460985692213155E-5	0.2278158940869383	2.2845140435724118E-5
98-99	0.13926189926522553	1.8276112348579294E-4	2.9075633281830694E-5	1.2460985692213155E-5	0.2282894115432424	2.492197138442631E-5
100-101	0.13928266757471253	1.8276112348579294E-4	2.9075633281830694E-5	1.2460985692213155E-5	0.2287816204780848	2.492197138442631E-5
102-103	0.13929928222230215	1.8276112348579294E-4	3.5306126127937274E-5	1.2460985692213155E-5	0.22925098427249152	2.492197138442631E-5
104-105	0.13931382003894308	1.8276112348579294E-4	3.7382957076639467E-5	1.2460985692213155E-5	0.22983872743097422	2.492197138442631E-5
106-107	0.1393387420103275	1.8691478538319733E-4	3.7382957076639467E-5	1.2460985692213155E-5	0.2303371668586628	2.492197138442631E-5
108-109	0.13936158715076324	1.931452782293039E-4	4.5690280871448235E-5	1.2460985692213155E-5	0.23087091241247923	2.492197138442631E-5
110-111	0.13939689327689117	1.972989401267083E-4	4.5690280871448235E-5	1.4537816640915349E-5	0.23144411775432103	2.492197138442631E-5
112-113	0.13940935426258338	2.0352943297281486E-4	4.5690280871448235E-5	1.661464758961754E-5	0.23201732309616285	2.492197138442631E-5
114-115	0.1394238920792243	2.0352943297281486E-4	4.5690280871448235E-5	1.661464758961754E-5	0.23248253322867213	2.6998802333128502E-5
116-117	0.1394405067268139	2.0768309487021925E-4	4.5690280871448235E-5	1.661464758961754E-5	0.23304950807766783	3.322929517923508E-5
118-119	0.1394508908815574	2.1183675676762363E-4	4.984394276885262E-5	1.661464758961754E-5	0.23354794750535635	3.5306126127937274E-5
120-121	0.13945919820535221	2.1599041866502802E-4	4.984394276885262E-5	1.661464758961754E-5	0.23417307362091572	3.7382957076639467E-5
122-123	0.13947373602199314	2.1599041866502802E-4	4.984394276885262E-5	1.661464758961754E-5	0.23467982037239904	3.7382957076639467E-5
124-125	0.13948827383863405	2.201440805624324E-4	4.984394276885262E-5	1.661464758961754E-5	0.23532779162839412	3.7382957076639467E-5
126-127	0.13950073482432626	2.2637457340853896E-4	5.3997604666257004E-5	1.661464758961754E-5	0.2358636140131593	4.5690280871448235E-5
128-129	0.13951734947191588	2.3468189720334773E-4	5.3997604666257004E-5	1.661464758961754E-5	0.23643889618594982	4.984394276885262E-5
130-131	0.139544348274249	2.450660519468587E-4	5.3997604666257004E-5	1.661464758961754E-5	0.23718240166558519	5.6074435614959197E-5
132-133	0.13955473242899252	2.492197138442631E-4	5.3997604666257004E-5	1.661464758961754E-5	0.23779091313355494	6.230492846106578E-5
134-135	0.13956719341468474	2.492197138442631E-4	5.3997604666257004E-5	1.661464758961754E-5	0.23826443058985902	6.230492846106578E-5
136-137	0.13957550073847955	2.533733757416675E-4	5.3997604666257004E-5	1.661464758961754E-5	0.2387774078341885	6.230492846106578E-5
138-139	0.13960042270986397	2.533733757416675E-4	5.3997604666257004E-5	1.661464758961754E-5	0.23933607535938936	7.061225225587455E-5
>>END_MODULE
