##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.2d.S.3.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24094736
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.82330497416531	36.0	34.0	38.0	30.0	39.0
2	34.69579467482026	35.0	34.0	38.0	31.0	39.0
3	34.89971963170711	35.0	34.0	38.0	31.0	39.0
4	34.839362464896894	36.0	34.0	38.0	31.0	39.0
5	34.7116888103692	35.0	34.0	37.0	31.0	39.0
6	34.71932890237934	35.0	34.0	37.0	31.0	39.0
7	34.74246661179438	35.0	34.0	37.0	32.0	39.0
8	34.88663457445643	35.0	34.0	37.0	31.0	39.0
9	34.86603194158259	35.0	34.0	37.0	31.0	39.0
10-14	34.83561543068992	35.0	34.0	37.4	31.0	39.0
15-19	34.83784668153244	35.0	34.0	37.0	31.0	39.0
20-24	34.84141073801349	35.0	34.0	37.0	31.0	39.0
25-29	34.838977609051206	35.0	34.0	37.0	31.0	39.0
30-34	34.81855425184987	35.0	34.0	37.0	31.0	39.0
35-39	34.81478464009732	35.0	34.0	37.0	31.0	39.0
40-44	34.746999029165536	35.0	34.0	37.0	31.0	39.0
45-49	34.64589060448722	35.0	34.0	37.0	31.0	39.0
50-54	34.609846656962745	35.0	34.0	37.0	30.2	39.0
55-59	34.60164276545716	35.0	34.0	37.0	30.0	39.0
60-64	34.57032638996335	35.0	34.0	37.0	30.0	39.0
65-69	34.553849156097826	35.0	34.0	37.0	30.0	39.0
70-74	34.526992875124265	35.0	34.0	37.0	30.0	39.0
75-79	34.506106661637624	35.0	34.0	37.0	30.0	39.0
80-84	34.4773133517628	35.0	34.0	37.0	30.0	39.0
85-89	34.46082359233984	35.0	34.0	37.0	30.0	39.0
90-94	34.431557316087634	35.0	34.0	37.0	30.0	39.0
95-99	34.39646080372078	35.0	34.0	37.0	30.0	39.0
100-104	34.35925592212341	35.0	34.0	37.0	30.0	39.0
105-109	34.30293750468983	35.0	34.0	37.0	30.0	39.0
110-114	34.27498830449938	35.0	34.0	37.0	30.0	39.0
115-119	34.223875588427276	35.0	34.0	37.0	29.2	39.0
120-124	34.1873262359048	35.0	34.0	37.0	29.0	39.0
125-129	34.13316635633608	35.0	34.0	37.0	29.0	39.0
130-134	34.087058957607994	35.0	34.0	37.0	29.0	39.0
135-139	34.03521241320096	35.0	34.0	37.0	29.0	39.0
140-144	34.00647919113951	35.0	34.0	37.0	29.0	39.0
145-149	33.93819336306486	35.0	34.0	37.0	28.2	39.0
150	33.90774088581008	35.0	34.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	40.0
22	442.0
23	4381.0
24	26004.0
25	96428.0
26	232673.0
27	410936.0
28	590671.0
29	764129.0
30	943246.0
31	1174154.0
32	1487816.0
33	1938733.0
34	2662441.0
35	4131938.0
36	8173799.0
37	1456897.0
38	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.617067670773764	10.45296752916966	14.291883410921125	38.63808138913545
2	22.737823907654953	12.683312280273038	36.70401295290296	27.874850859169054
3	21.670882736806202	17.291042229886695	28.046675633779078	32.99139939952802
4	27.036497868703364	25.13731430476766	21.672335543028883	26.153852283500097
5	26.818311439127502	30.650651381001097	23.764764171884718	18.766273007986683
6	19.751709036608236	36.51091121019692	25.172138371422946	18.565241381771894
7	16.75941601733959	23.746366834050725	40.24440158760304	19.249815561006642
8	19.38627459289105	23.63064069189939	31.05596965509078	25.927115060118783
9	20.22717098862431	22.38528334472034	32.24376931803382	25.14377634862153
10-14	21.196506182020464	28.49245979677336	26.43647447811272	23.874559543093458
15-19	21.44214799372641	27.477587366832584	27.121903723935624	23.95836091550538
20-24	21.38636824413009	27.451292494053153	27.052346585005537	24.109992676811224
25-29	21.309441083205325	27.764882569836264	26.645085557282535	24.28059078967588
30-34	21.351891669093774	27.544572438080888	26.854086291581385	24.249449601243953
35-39	21.59551148830689	27.31976122790937	26.780762875762086	24.30396440802165
40-44	21.781292866410837	27.38274667675916	26.586408993231103	24.2495514635989
45-49	22.042546282527216	27.32720734686742	26.53828253186597	24.091963838739392
50-54	21.99849574657157	27.560884134992147	26.430832236497437	24.00978788193884
55-59	21.927002625970296	27.48177492745419	26.54761682523155	24.043605621343964
60-64	22.195561748753224	27.332768970835687	26.2624156456916	24.209253634719484
65-69	22.02463530461468	27.46769528533306	26.46676712932914	24.040902280723124
70-74	22.390749676762354	27.214529608315974	26.377869410082145	24.016851304839523
75-79	22.219495478748048	27.144625037868327	26.36897982250502	24.266899660878607
80-84	22.47493896056933	27.07755254263484	26.439330090873618	24.008178405922205
85-89	22.32952976971676	27.09201359714305	26.431070187208118	24.147386445932074
90-94	22.34182425338898	26.829658987677945	26.407170149866143	24.421346609066934
95-99	22.538605064663617	26.895340106529154	26.62279426666713	23.943260562140104
100-104	22.51650712541484	26.704899315055542	26.63973540455819	24.138858154971423
105-109	22.58972560555671	26.713822486794136	26.51982855827759	24.176623349371567
110-114	22.520701769880397	26.763367131805328	26.51428558147631	24.20164551683797
115-119	22.637961073310922	27.00917385196739	26.374770659926256	23.978094414795432
120-124	22.58002767797406	26.82555361820786	26.41700870548093	24.177409998337144
125-129	22.956136111538335	26.78387853848612	26.31385325434048	23.946132095635058
130-134	22.632304981759184	27.04097541395145	26.22268250606874	24.104037098220623
135-139	22.78788112109679	26.803272490983847	26.248840958674997	24.16000542924436
140-144	22.75055680091482	26.648159469372395	26.544621339145884	24.056662390566903
145-149	22.558048429223305	27.057659455579003	26.552274277708538	23.83201783748915
150	22.304595005126238	26.626577014171147	26.62775569842998	24.441072282272636
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.0
10	0.5
11	2.5
12	8.0
13	14.5
14	19.5
15	36.0
16	73.5
17	175.5
18	427.0
19	783.0
20	1243.5
21	2027.0
22	3500.5
23	5970.0
24	9771.0
25	14934.0
26	21127.5
27	30710.0
28	47811.0
29	71022.5
30	97063.5
31	122817.0
32	148743.0
33	182455.0
34	220450.5
35	267632.5
36	337610.0
37	412601.0
38	484656.0
39	590192.5
40	736847.0
41	895178.0
42	1054388.5
43	1210215.0
44	1346044.0
45	1446026.0
46	1527580.5
47	1561336.5
48	1513636.0
49	1460998.0
50	1376141.5
51	1227783.0
52	1055701.0
53	901883.5
54	758695.5
55	629744.0
56	544654.5
57	457216.0
58	376507.5
59	307873.0
60	239081.5
61	166020.5
62	95517.5
63	53684.5
64	33979.0
65	20487.0
66	12808.0
67	5813.5
68	2066.5
69	957.0
70	783.5
71	559.0
72	302.5
73	141.0
74	83.5
75	54.5
76	28.0
77	19.0
78	13.0
79	3.5
80	2.5
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.9427699062567024E-4
2	2.4901704671094965E-4
3	9.545653457253071E-5
4	5.8103977565888247E-5
5	2.0751420559245803E-5
6	8.715596634883238E-5
7	1.9921363736875972E-4
8	7.885539812513405E-5
9	9.130625046068154E-5
10-14	1.6850153494107594E-4
15-19	2.7391875138204464E-4
20-24	1.701616485858156E-4
25-29	3.4613369492822E-4
30-34	8.499781861067081E-4
35-39	9.470948343239786E-4
40-44	0.0010749235849689326
45-49	9.213630728305137E-4
50-54	5.221057412706244E-4
55-59	4.1253824071780653E-4
60-64	2.3573613755303234E-4
65-69	3.3036261530319317E-4
70-74	3.3036261530319317E-4
75-79	2.498471035333195E-4
80-84	2.6976846727019545E-4
85-89	3.577544904413977E-4
90-94	3.635648881979865E-4
95-99	3.0546091063209823E-4
100-104	4.5985147959288705E-4
105-109	2.67278296803086E-4
110-114	2.3656619437540218E-4
115-119	2.448667625991005E-4
120-124	2.216251715727452E-4
125-129	1.33639148401543E-4
130-134	1.602009667173776E-4
135-139	1.602009667173776E-4
140-144	1.5688073942789828E-4
145-149	1.8427261456610273E-4
150	3.029707401649887E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4094736E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.441830669188676
#Duplication Level	Percentage of total
1	32.20142335159335
2	8.566780503189062
3	4.928125540362886
4	3.599219205285763
5	2.6449969851111628
6	2.076934828481503
7	1.7059074804228962
8	1.4677635047437203
9	1.2065271902145316
>10	15.724649749407286
>50	5.207645222948823
>100	11.109059518081416
>500	4.096110999270382
>1k	4.883897862810084
>5k	0.4701973018571272
>10k+	0.11076075622013408
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.150284111849161E-6	2.4901704671094965E-5	0.0	1.2450852335547482E-5	2.739187513820446E-4	1.2450852335547482E-5
2	2.4901704671094965E-5	7.885539812513406E-5	0.0	2.4901704671094965E-5	0.0023615116596421726	3.320227289479329E-5
3	3.320227289479329E-5	8.715596634883238E-5	0.0	2.4901704671094965E-5	0.004623416500599965	5.395369345403909E-5
4	8.715596634883238E-5	9.54565345725307E-5	0.0	2.9051988782944127E-5	0.006831367648103719	5.810397756588825E-5
5	1.0790738690807818E-4	9.54565345725307E-5	0.0	2.9051988782944127E-5	0.00786478839195416	6.225426167773741E-5
6	1.5356051213841895E-4	9.54565345725307E-5	0.0	2.9051988782944127E-5	0.009412844365673896	6.225426167773741E-5
7	2.0336392148060888E-4	1.162079551317765E-4	0.0	2.9051988782944127E-5	0.010823940963702611	6.640454578958658E-5
8	3.3202272894793285E-4	1.2035823924362566E-4	0.0	3.320227289479329E-5	0.014442988709235079	7.055482990143573E-5
9	4.108781270730669E-4	1.2035823924362566E-4	0.0	3.320227289479329E-5	0.016916558039897178	7.47051140132849E-5
10-11	4.897335251982009E-4	1.4110965980287146E-4	0.0	3.5277414950717864E-5	0.029425514353010548	8.715596634883238E-5
12-13	5.250109401489188E-4	1.4110965980287146E-4	0.0	3.735255700664245E-5	0.03703298513003006	9.960681868437986E-5
14-15	5.519877868759384E-4	1.494102280265698E-4	0.0	3.735255700664245E-5	0.04266492066980937	1.0790738690807818E-4
16-17	5.976409121062792E-4	1.5356051213841895E-4	0.0	3.735255700664245E-5	0.04629019384150962	1.0998252896400276E-4
18-19	6.910223046228852E-4	1.597859383061927E-4	0.0	3.735255700664245E-5	0.04933235209549505	1.1205767101992734E-4
20-21	0.0010583224485215359	2.0543906353653348E-4	0.0	3.942769906256703E-5	0.05182459770466047	1.1205767101992734E-4
22-23	0.0014173220241964885	2.1581477381615635E-4	0.0	4.357798317441619E-5	0.05422761220542113	1.1205767101992734E-4
24-25	0.0015335299793282648	2.2411534203985468E-4	0.0	4.565312523034077E-5	0.056757210371593196	1.1205767101992734E-4
26-27	0.0016082350933415497	2.2411534203985468E-4	0.0	4.772826728626535E-5	0.05899006322376805	1.2450852335547482E-4
28-29	0.0017721713157595916	2.32415910263553E-4	0.0	5.3953693454039095E-5	0.06167322190207853	1.3280909157917313E-4
30-31	0.0020191132204146166	2.32415910263553E-4	0.0	7.055482990143573E-5	0.06458049592242887	1.4318480185879603E-4
32-33	0.0021062691867634493	2.32415910263553E-4	0.0	7.47051140132849E-5	0.06707274153159429	1.5148537008249436E-4
34-35	0.0021955002951682063	2.3656619437540215E-4	0.0	9.54565345725307E-5	0.06969364594822702	1.597859383061927E-4
36-37	0.002234927994230773	2.3656619437540215E-4	0.0	1.1205767101992734E-4	0.0720800593125403	1.6186108036211726E-4
38-39	0.0022411534203985468	2.3656619437540215E-4	0.0	1.2450852335547482E-4	0.07435856528994549	1.6393622241804183E-4
40-41	0.0022556794147900188	2.3656619437540215E-4	0.0	1.2450852335547482E-4	0.07651671302810706	1.6601136447396642E-4
42-43	0.0022702054091814907	2.3656619437540215E-4	0.0	1.3280909157917315E-4	0.07871843874944304	1.701616485858156E-4
44-45	0.0022868065456288873	2.4071647848725132E-4	0.0	1.3280909157917315E-4	0.08122936063711178	1.7431193269766475E-4
46-47	0.0023117082502999826	2.4071647848725132E-4	0.0	1.3280909157917315E-4	0.08502064517328597	1.7638707475358935E-4
48-49	0.0023158585344118317	2.448667625991005E-4	0.0	1.3695937569102232E-4	0.08729707600863525	1.8676278503321225E-4
50-51	0.002334534812915153	2.4901704671094965E-4	0.0	1.4525994391472062E-4	0.08967518880472482	1.909130691450614E-4
52-53	0.002369812227865871	2.4901704671094965E-4	0.0	1.4525994391472062E-4	0.09177730770737641	2.012887794246843E-4
54-55	0.002392638790481041	2.4901704671094965E-4	0.0	1.4525994391472062E-4	0.09345194734650755	2.0958934764838262E-4
56-57	0.0024901704671094964	2.5316733082279884E-4	0.0	1.494102280265698E-4	0.09543578315197146	2.2619048409577928E-4
58-59	0.002537898734395762	2.57317614934648E-4	0.0	1.5563565419434355E-4	0.09763543373125151	2.32415910263553E-4
60-61	0.002679008394198633	2.5939275699057255E-4	0.0	1.5771079625026812E-4	0.09975622891240643	2.3656619437540218E-4
62-63	0.002735037229708597	2.614678990464971E-4	0.0	1.5771079625026812E-4	0.10182307040010731	2.4071647848725132E-4
64-65	0.0027557886502678425	2.614678990464971E-4	0.0	1.6186108036211726E-4	0.1045415064933685	2.427916205431759E-4
66-67	0.002788990923162636	2.780690354938938E-4	0.0	1.6601136447396642E-4	0.10699224926141543	2.448667625991005E-4
68-69	0.0028221931960574296	2.9051988782944125E-4	0.0	1.6601136447396642E-4	0.1088121488444613	2.448667625991005E-4
70-71	0.002859545753064072	2.988204560531396E-4	0.0	1.701616485858156E-4	0.11070882868357637	2.4901704671094965E-4
72-73	0.0028761468895114684	2.988204560531396E-4	0.0	1.7431193269766475E-4	0.11218010440122689	2.531673308227988E-4
74-75	0.0029051988782944124	2.988204560531396E-4	0.0	1.9506335325691055E-4	0.1137758886422329	2.531673308227988E-4
76-77	0.002932175725021432	3.029707401649887E-4	0.0	2.0543906353653345E-4	0.11512473097858388	2.531673308227988E-4
78-79	0.0029778288502517727	3.029707401649887E-4	0.0	2.1581477381615635E-4	0.11642584504764858	2.531673308227988E-4
80-81	0.0030151814072584154	3.112713083886871E-4	0.0	2.1581477381615635E-4	0.11784731735595692	2.57317614934648E-4
82-83	0.00304008311192951	3.237221607242345E-4	0.0	2.1788991587208095E-4	0.1194078241820122	2.656181831583463E-4
84-85	0.003075360526880228	3.3202272894793285E-4	0.0	2.2204019998393009E-4	0.12097040615012342	2.676933252142709E-4
86-87	0.0031085627997750214	3.3617301305978204E-4	0.0	2.2411534203985468E-4	0.12237320217992842	2.6976846727019545E-4
88-89	0.003114788225942795	3.403232971716312E-4	0.0	2.2411534203985468E-4	0.12381542590879602	2.6976846727019545E-4
90-91	0.0031313893623901917	3.4447358128348037E-4	0.0	2.2411534203985468E-4	0.12591131938527983	2.6976846727019545E-4
92-93	0.0031625164932290607	3.4447358128348037E-4	0.0	2.2411534203985468E-4	0.1278142246505627	2.6976846727019545E-4
94-95	0.0032185453287390245	3.4654872333940494E-4	0.0	2.2411534203985468E-4	0.12920872011214402	2.6976846727019545E-4
96-97	0.0032994758689200827	3.5484929156310327E-4	0.0	2.2411534203985468E-4	0.1305949150055016	2.6976846727019545E-4
98-99	0.0033700306988215184	3.673001438986507E-4	4.150284111849161E-6	2.2619048409577928E-4	0.1317071911474772	2.7184360932612E-4
100-101	0.0034903889380651443	3.7352557006642444E-4	4.150284111849161E-6	2.2826562615170385E-4	0.13290247297168975	2.739187513820446E-4
102-103	0.0035235912109599374	3.7560071212234907E-4	4.150284111849161E-6	2.3034076820762842E-4	0.1339358937155402	2.739187513820446E-4
104-105	0.0035484929156310322	3.7767585417827364E-4	4.150284111849161E-6	2.3864133643132675E-4	0.13500251673228542	2.780690354938938E-4
106-107	0.0036231980296443176	3.7767585417827364E-4	4.150284111849161E-6	2.448667625991005E-4	0.13606706460697474	2.780690354938938E-4
108-109	0.0036916777174898284	3.818261382901228E-4	4.150284111849161E-6	2.448667625991005E-4	0.13700502881625265	2.780690354938938E-4
110-111	0.0037352557006642443	3.818261382901228E-4	4.150284111849161E-6	2.448667625991005E-4	0.1379554438778661	2.822193196057429E-4
112-113	0.0038348625193486245	3.818261382901228E-4	4.150284111849161E-6	2.4901704671094965E-4	0.13903244260489095	2.822193196057429E-4
114-115	0.0038742902184111918	3.818261382901228E-4	4.150284111849161E-6	2.4901704671094965E-4	0.14014471874686654	2.9051988782944125E-4
116-117	0.003953145616536325	3.9012670651382116E-4	4.150284111849161E-6	2.531673308227988E-4	0.1409332727281179	2.9051988782944125E-4
118-119	0.004009174452046289	4.0880298501714234E-4	4.150284111849161E-6	2.57317614934648E-4	0.1417571041243199	2.9051988782944125E-4
120-121	0.004162734964184708	4.1295326912899153E-4	4.150284111849161E-6	2.656181831583463E-4	0.14274694688499595	2.9051988782944125E-4
122-123	0.004405526584727884	4.150284111849161E-4	4.150284111849161E-6	2.7184360932612E-4	0.14372433879333646	2.9051988782944125E-4
124-125	0.004530035108083358	4.150284111849161E-4	4.150284111849161E-6	2.780690354938938E-4	0.14462910072971957	2.9051988782944125E-4
126-127	0.004581913659481474	4.150284111849161E-4	4.150284111849161E-6	2.780690354938938E-4	0.14556498979694155	2.9467017194129044E-4
128-129	0.0046649193417184565	4.1710355324084067E-4	4.150284111849161E-6	2.780690354938938E-4	0.14634109292585734	2.96745313997215E-4
130-131	0.004808104143577253	4.2747926352046357E-4	4.150284111849161E-6	2.822193196057429E-4	0.14697401125291432	3.029707401649887E-4
132-133	0.0048848843996464626	4.2955440557638813E-4	4.150284111849161E-6	2.842944616616675E-4	0.14766088327342536	3.1127130838868705E-4
134-135	0.004990716644498615	4.316295476323127E-4	4.150284111849161E-6	2.884447457735167E-4	0.14841000955561412	3.1127130838868705E-4
136-137	0.005127676020189638	4.357798317441619E-4	4.150284111849161E-6	2.9051988782944125E-4	0.14921931495742474	3.1334645044461167E-4
138-139	0.005223132554762169	4.4408039996786017E-4	4.150284111849161E-6	2.9051988782944125E-4	0.15007219834240973	3.1542159250053624E-4
>>END_MODULE
