##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.4d.R.1.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23543034
Total Bases	3.5 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.084756790479936	37.0	35.0	38.0	33.0	39.0
2	35.57878687173454	35.0	35.0	38.0	33.0	39.0
3	35.6892346160652	36.0	35.0	38.0	34.0	39.0
4	35.792505970131124	36.0	35.0	38.0	34.0	39.0
5	35.72244295276471	36.0	35.0	38.0	33.0	39.0
6	35.64794932547776	36.0	35.0	37.0	33.0	39.0
7	35.60286932431903	35.0	34.0	38.0	33.0	39.0
8	35.78078373416103	36.0	35.0	38.0	33.0	39.0
9	35.75618609733988	36.0	35.0	38.0	33.0	39.0
10-14	35.68284299296344	36.0	35.0	37.8	33.0	39.0
15-19	35.68853915769735	36.0	35.0	38.0	33.0	39.0
20-24	35.6714115266537	36.0	35.0	38.0	33.0	39.0
25-29	35.65657223278869	36.0	35.0	38.0	33.0	39.0
30-34	35.6314914466844	36.0	35.0	38.0	33.0	39.0
35-39	35.61000315422388	36.0	35.0	38.0	33.0	39.0
40-44	35.592626923106	36.0	35.0	38.0	33.0	39.0
45-49	35.56157286269901	36.0	35.0	38.0	33.0	39.0
50-54	35.54330028151852	36.0	35.0	38.0	33.0	39.0
55-59	35.51422335795803	36.0	35.0	38.0	33.0	39.0
60-64	35.47867283375626	36.0	34.8	38.0	33.0	39.0
65-69	35.443417590103294	36.0	34.0	38.0	33.0	39.0
70-74	35.415860530125386	36.0	34.0	38.0	33.0	39.0
75-79	35.37300681806771	36.0	34.0	38.0	32.4	39.0
80-84	35.33616540671861	36.0	34.0	38.0	32.0	39.0
85-89	35.29360399343602	36.0	34.0	38.0	32.0	39.0
90-94	35.2404643003956	36.0	34.0	38.0	32.0	39.0
95-99	35.179678719403796	36.0	34.0	38.0	32.0	39.0
100-104	35.13069467597082	36.0	34.0	38.0	32.0	39.0
105-109	35.067801286784025	36.0	34.0	38.0	32.0	39.0
110-114	35.00838526589224	36.0	34.0	37.8	31.6	39.0
115-119	34.94029433079866	35.4	34.0	37.0	31.0	39.0
120-124	34.865964335777626	35.0	34.0	37.0	31.0	39.0
125-129	34.80639135125915	35.0	34.0	37.0	31.0	39.0
130-134	34.717290167444006	35.0	34.0	37.0	30.8	39.0
135-139	34.62711452568093	35.0	34.0	37.0	30.0	39.0
140-144	34.526587278428096	35.0	34.0	37.0	30.0	39.0
145-149	34.43906068351258	35.0	34.0	37.0	30.0	39.0
150	34.372678602086715	35.0	34.0	37.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	20.0
21	210.0
22	1558.0
23	7718.0
24	25788.0
25	62710.0
26	123106.0
27	211132.0
28	306664.0
29	398930.0
30	511826.0
31	667963.0
32	905139.0
33	1303046.0
34	2058184.0
35	3778394.0
36	1.1288478E7
37	1892166.0
38	2.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.267919781955015	19.201558235999507	16.791719944992643	30.73880203705283
2	24.853233650906407	25.2999135667864	32.45576302764389	17.391089754663298
3	20.43495753351076	29.258973163781693	30.185026279960347	20.121043022747195
4	24.0473636490522	33.4720155439609	22.486290424590134	19.994330382396765
5	25.78818006209395	35.02949534881528	22.297253616504992	16.885070972585776
6	19.248419723643096	39.44777890564147	22.37617292656503	18.9276284441504
7	20.700522286125057	21.174441662871487	37.76142870965569	20.363607341347763
8	21.707615157316393	23.709421806442695	28.294370568142174	26.288592468098738
9	23.808596632022873	23.121875455814234	28.849093961296578	24.220433950866315
10-14	23.186790672653316	29.544113340880422	25.58586475016021	21.68323123630605
15-19	23.107975149744718	28.481773661642535	27.041062142127153	21.36918904648559
20-24	23.183108005535736	28.52944102276707	26.949052530782563	21.338398440914624
25-29	23.297491931562362	28.411910957711832	26.871257348320164	21.41933976240564
30-34	23.25708232847134	28.212100445507577	27.103810834236576	21.42700639178451
35-39	23.355007883478464	28.139327566186477	27.07081616643646	21.434848383898593
40-44	23.471182176434862	27.909576140441374	27.218962517745165	21.4002791653786
45-49	23.472402070183477	27.724608476545548	27.29964710580633	21.503342347464645
50-54	23.50840887386747	27.69701012532217	27.27177945326468	21.522801547545683
55-59	23.671447783662888	27.58544714330362	27.193392321482442	21.549712751551052
60-64	23.559257485674955	27.557365800856427	27.25931840390665	21.62405830956197
65-69	23.663814103144055	27.472282459431526	27.230053696562646	21.633849740861777
70-74	23.743599911549207	27.413572949009037	27.136829518234567	21.70599762120719
75-79	23.673569407809875	27.496857499281656	27.201186171691994	21.628386921216475
80-84	23.79755982172901	27.51847701532436	27.04640871690539	21.63755444604124
85-89	23.803458806541247	27.51038035284662	27.102330141476244	21.583830699135888
90-94	23.762358953075964	27.436378229215304	27.13816347662042	21.66309934108832
95-99	23.80033803632429	27.540038173502886	27.156308499262117	21.503315290910706
100-104	24.014987397248692	27.50679736100961	26.94611600990863	21.532099231833072
105-109	23.769662174974236	27.64378650299521	27.085559381051393	21.500991940979162
110-114	23.82090111088402	27.63604748339613	26.998903599246415	21.544147806473436
115-119	23.876744178341667	27.633820687681972	27.04679099558706	21.442644138389298
120-124	23.849244748998792	27.560552306045178	27.179397523700644	21.41080542125539
125-129	23.8085541566138	27.67010318211323	27.139070520817327	21.382272140455644
130-134	23.897925815338837	27.558183877235194	27.20638639862645	21.337503908799523
135-139	23.774071770019106	27.545904236471813	27.321844754588554	21.358179238920524
140-144	23.819985988212057	27.676471095441652	27.25902872161676	21.244514194729533
145-149	23.963651413832217	28.318841998019455	26.653805962307153	21.06370062584117
150	23.55242743989581	28.212030785836696	26.81743992724132	21.418101847026175
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	3.0
2	13.5
3	23.5
4	35.0
5	45.5
6	44.5
7	39.5
8	35.5
9	36.0
10	28.5
11	18.5
12	20.5
13	24.5
14	29.0
15	44.0
16	70.0
17	130.0
18	233.0
19	405.5
20	651.0
21	1015.5
22	1525.5
23	2506.5
24	4232.5
25	6899.0
26	10594.5
27	16624.5
28	26319.0
29	39463.0
30	54673.5
31	73074.5
32	99237.0
33	134653.0
34	180070.0
35	248533.0
36	350593.5
37	471839.5
38	615297.5
39	800213.5
40	1015283.5
41	1215074.0
42	1390890.0
43	1551087.0
44	1663470.0
45	1713761.0
46	1704888.0
47	1644635.5
48	1537107.0
49	1385972.5
50	1203275.5
51	1008448.5
52	821923.0
53	652974.0
54	508272.0
55	384110.5
56	282978.0
57	209901.0
58	154744.0
59	111172.0
60	77347.5
61	52280.5
62	35840.0
63	24586.5
64	16333.5
65	10741.0
66	7425.5
67	5596.5
68	4830.5
69	3585.5
70	1988.5
71	1258.5
72	660.5
73	423.5
74	371.5
75	262.5
76	171.5
77	51.5
78	12.5
79	2.0
80	0.0
81	0.0
82	0.5
83	0.5
84	0.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.495081814858696E-6
2	4.247540907429348E-6
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	4.247540907429348E-6
9	0.0
10-14	0.09681504941121863
15-19	8.495081814858698E-7
20-24	0.0
25-29	8.495081814858698E-7
30-34	0.0
35-39	8.495081814858698E-7
40-44	0.0
45-49	0.0
50-54	1.6990163629717395E-6
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	1.6990163629717395E-6
80-84	0.0
85-89	0.0
90-94	8.495081814858698E-7
95-99	8.495081814858696E-6
100-104	8.495081814858698E-7
105-109	8.495081814858698E-7
110-114	8.495081814858698E-7
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.3543034E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.912476749773326
#Duplication Level	Percentage of total
1	25.673502030693722
2	11.490253644212396
3	7.645258300969345
4	5.449708598106654
5	4.064309440597249
6	3.2686343393675488
7	2.6805537340588588
8	2.3829547782812615
9	1.932238507364225
>10	22.017073371328106
>50	5.4980463774616695
>100	5.96792948005715
>500	0.9179852342506727
>1k	0.8990781118208597
>5k	0.06703593727722545
>10k+	0.04543811415316489
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.247540907429348E-6	4.247540907429348E-6	0.0	0.0	0.0016140655448231523	0.0
2	1.6990163629717393E-5	1.2742622722288044E-5	0.0	0.0	0.005611001538714169	0.0
3	5.097049088915218E-5	1.2742622722288044E-5	0.0	0.0	0.007072155610869865	0.0
4	1.061885226857337E-4	1.6990163629717393E-5	0.0	0.0	0.008456853946691833	0.0
5	1.061885226857337E-4	1.6990163629717393E-5	0.0	0.0	0.010126137523311566	0.0
6	1.146836045005924E-4	2.1237704537146742E-5	0.0	0.0	0.011778430936301583	0.0
7	1.2742622722288044E-4	3.3980327259434785E-5	0.0	0.0	0.013774775162793377	0.0
8	1.9113934083432067E-4	3.8227868166864135E-5	0.0	0.0	0.018935537365320033	0.0
9	1.9963442264917937E-4	5.097049088915218E-5	0.0	0.0	0.024877847094813693	4.247540907429348E-6
10-11	2.3998606126975818E-4	5.097049088915218E-5	0.0	0.0	0.03101979124695653	4.247540907429348E-6
12-13	2.718426180754783E-4	5.309426134286685E-5	0.0	0.0	0.03687927392875531	4.247540907429348E-6
14-15	3.270606498720598E-4	5.946557270401087E-5	0.0	0.0	0.04554850492081862	4.247540907429348E-6
16-17	3.695360589463533E-4	6.58368840651549E-5	0.0	0.0	0.05098960482323561	4.247540907429348E-6
18-19	3.9714507484464405E-4	7.220819542629892E-5	0.0	8.495081814858696E-6	0.05887941205878563	4.247540907429348E-6
20-21	4.247540907429348E-4	8.28270476948723E-5	0.0	8.495081814858696E-6	0.06451377507249066	4.247540907429348E-6
22-23	4.778483520858017E-4	9.7693440870875E-5	8.495081814858696E-6	8.495081814858696E-6	0.06988266677948135	4.247540907429348E-6
24-25	5.224475316138098E-4	1.1043606359316305E-4	8.495081814858696E-6	8.495081814858696E-6	0.0751878453728606	4.247540907429348E-6
26-27	5.500565475121006E-4	1.1893114540802175E-4	8.495081814858696E-6	8.495081814858696E-6	0.07874091334192526	4.247540907429348E-6
28-29	6.668639224664077E-4	1.1893114540802175E-4	8.495081814858696E-6	8.495081814858696E-6	0.08248724442227795	4.247540907429348E-6
30-31	8.15527854226435E-4	1.3592130903773914E-4	1.0618852268573371E-5	8.495081814858696E-6	0.08711281647046851	4.247540907429348E-6
32-33	9.429540814493154E-4	1.4016884994516848E-4	1.2742622722288044E-5	8.495081814858696E-6	0.09164069507778819	4.247540907429348E-6
34-35	0.001055513915496193	1.5291147266745654E-4	1.2742622722288044E-5	8.495081814858696E-6	0.09445681469941386	4.247540907429348E-6
36-37	0.0010979893245704865	1.5715901357488588E-4	1.6990163629717393E-5	8.495081814858696E-6	0.0977932580821996	4.247540907429348E-6
38-39	0.0011617024381819266	1.635303249360299E-4	1.6990163629717393E-5	8.495081814858696E-6	0.10090670556734531	4.247540907429348E-6
40-41	0.0012296630927007965	1.6777786584345924E-4	1.6990163629717393E-5	1.0618852268573371E-5	0.10354867601176637	6.371311361144022E-6
42-43	0.0012445294858767992	1.7839671811203264E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.10596340301763996	8.495081814858696E-6
44-45	0.0012593958790528019	1.89015570380606E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.1081402677326975	1.2742622722288044E-5
46-47	0.0013209852222105273	2.1874835673261144E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.11023430540006016	1.2742622722288044E-5
48-49	0.0013762032540071089	2.3998606126975818E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.11238568486967312	1.2742622722288044E-5
50-51	0.0014250499744425465	2.442336021771875E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.11409319631445972	1.2742622722288044E-5
52-53	0.0014484114494334077	2.463573726309022E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.11594300037964521	1.2742622722288044E-5
54-55	0.0014845155471465573	2.484811430846169E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.11783103231299755	1.2742622722288044E-5
56-57	0.0015737139062025735	2.527286839920462E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.1198210052281282	1.2742622722288044E-5
58-59	0.0016437983311751577	2.569762248994756E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.1216899232273971	1.2742622722288044E-5
60-61	0.0017245016084163154	2.6971884762176364E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.12300666090870022	1.2742622722288044E-5
62-63	0.0017712245583980382	2.782139294366223E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.12479487563072797	1.2742622722288044E-5
64-65	0.0018030811152037582	3.0369917488119844E-4	1.6990163629717393E-5	1.6990163629717393E-5	0.12632611412785624	1.2742622722288044E-5
66-67	0.0018604229174540546	3.440508135017772E-4	1.6990163629717393E-5	2.1237704537146742E-5	0.12755365345010333	1.2742622722288044E-5
68-69	0.001877413081083772	3.546696657703506E-4	1.6990163629717393E-5	2.1237704537146742E-5	0.12885977227913786	1.4866393176002718E-5
70-71	0.0018859081628986307	3.567934362240653E-4	1.6990163629717393E-5	2.1237704537146742E-5	0.13008731160138493	1.6990163629717393E-5
72-73	0.0019262598015192096	3.6528851803892395E-4	1.6990163629717393E-5	2.1237704537146742E-5	0.1309962853555748	2.7609015898290764E-5
74-75	0.0019538688174175	3.737835998537827E-4	1.6990163629717393E-5	2.1237704537146742E-5	0.132096398450599	2.973278635200544E-5
76-77	0.001981477833315791	3.8227868166864135E-4	1.6990163629717393E-5	2.1237704537146742E-5	0.13314129351382664	2.973278635200544E-5
78-79	0.002038819635566087	4.077639271132175E-4	2.1237704537146742E-5	2.1237704537146742E-5	0.13443254594968518	2.973278635200544E-5
80-81	0.00205368602874209	4.3112540210407885E-4	2.1237704537146742E-5	2.1237704537146742E-5	0.13563672379694136	2.973278635200544E-5
82-83	0.002066428651464378	4.438680248263669E-4	2.1237704537146742E-5	2.1237704537146742E-5	0.13711274426227307	2.973278635200544E-5
84-85	0.0020897901264552393	4.544868770949403E-4	2.1237704537146742E-5	2.1237704537146742E-5	0.1385484130889842	3.185655680572011E-5
86-87	0.002096161437816383	4.6298195890979895E-4	2.1237704537146742E-5	2.1237704537146742E-5	0.13992886388389875	3.3980327259434785E-5
88-89	0.0021089040605386713	4.69353270270943E-4	2.1237704537146742E-5	2.1237704537146742E-5	0.14129020074472984	3.3980327259434785E-5
90-91	0.0021152753718998154	4.7997212253951635E-4	2.1237704537146742E-5	2.1237704537146742E-5	0.14215882286029913	3.3980327259434785E-5
92-93	0.002123770453714674	4.82095892993231E-4	2.548524544457609E-5	2.1237704537146742E-5	0.14306142530312788	3.610409771314946E-5
94-95	0.0021365130764369623	4.88467204354375E-4	2.548524544457609E-5	2.1237704537146742E-5	0.1441211867595315	4.035163862057881E-5
96-97	0.0021535032400666794	5.033335975303777E-4	2.548524544457609E-5	2.3361474990861417E-5	0.1450195416614528	4.2475409074293485E-5
98-99	0.0021662458627889677	5.139524497989512E-4	2.548524544457609E-5	2.548524544457609E-5	0.14592002033382784	5.73418022502962E-5
100-101	0.0021747409446038262	5.224475316138098E-4	2.548524544457609E-5	2.548524544457609E-5	0.14679501376075826	5.946557270401088E-5
102-103	0.0021747409446038262	5.288188429749539E-4	2.9732786352005435E-5	2.548524544457609E-5	0.14768274981041102	6.371311361144022E-5
104-105	0.0021789884855112555	5.309426134286685E-4	3.3980327259434785E-5	2.548524544457609E-5	0.14847916373055403	7.008442497258424E-5
106-107	0.0022044737309558316	5.500565475121006E-4	3.3980327259434785E-5	2.548524544457609E-5	0.14923734978253017	7.43319658800136E-5
108-109	0.0022087212718632614	5.67046711141818E-4	3.3980327259434785E-5	2.548524544457609E-5	0.14994031780270972	7.645573633372827E-5
110-111	0.002221463894585549	5.8616064522525E-4	3.3980327259434785E-5	2.548524544457609E-5	0.15081531122964015	8.070327724115762E-5
112-113	0.002236330287761552	5.989032679475381E-4	3.3980327259434785E-5	2.973278635200544E-5	0.1515883636747923	8.28270476948723E-5
114-115	0.002244825369576411	6.031508088549675E-4	3.3980327259434785E-5	2.973278635200544E-5	0.1524123866108336	9.1322129509731E-5
116-117	0.002255444221844984	6.031508088549675E-4	3.3980327259434785E-5	2.973278635200544E-5	0.15323003823551373	9.7693440870875E-5
118-119	0.002261815533206128	6.031508088549675E-4	4.2475409074293485E-5	2.973278635200544E-5	0.15397972920567501	9.7693440870875E-5
120-121	0.0022639393036598425	6.031508088549675E-4	4.2475409074293485E-5	2.973278635200544E-5	0.15492693082803177	9.7693440870875E-5
122-123	0.0022639393036598425	6.031508088549675E-4	4.2475409074293485E-5	2.973278635200544E-5	0.1556596316345633	9.7693440870875E-5
124-125	0.0022639393036598425	6.031508088549675E-4	4.2475409074293485E-5	2.973278635200544E-5	0.15644967424334516	9.7693440870875E-5
126-127	0.002268186844567272	6.031508088549675E-4	4.2475409074293485E-5	2.973278635200544E-5	0.1574244848816002	1.0406475223201904E-4
128-129	0.0022724343854747015	6.052745793086821E-4	4.2475409074293485E-5	2.973278635200544E-5	0.1583334586357901	1.061885226857337E-4
130-131	0.0022745581559284163	6.158934315772554E-4	4.459917952800816E-5	2.973278635200544E-5	0.15926367009451714	1.1043606359316305E-4
132-133	0.0022915483195581334	6.243885133921142E-4	4.6722949981722834E-5	2.973278635200544E-5	0.16008132171919728	1.1680737495430707E-4
134-135	0.002306414712734136	6.265122838458289E-4	5.097049088915218E-5	2.973278635200544E-5	0.16089897334387743	1.1893114540802175E-4
136-137	0.0023127860240952803	6.307598247532581E-4	5.309426134286685E-5	2.973278635200544E-5	0.1617081298867427	1.2105491586173644E-4
138-139	0.002317033565002709	6.413786770218316E-4	5.521803179658153E-5	2.973278635200544E-5	0.16244932577508914	1.231786863154511E-4
>>END_MODULE
