##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.4d.R.3.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24023071
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.90830801773845	37.0	35.0	38.0	33.0	39.0
2	35.410626060256824	35.0	34.0	37.0	33.0	39.0
3	35.54309867377073	35.0	34.0	37.0	33.0	39.0
4	35.632594891802135	36.0	35.0	38.0	33.0	39.0
5	35.55848380084295	36.0	35.0	37.0	33.0	39.0
6	35.49877357478567	36.0	35.0	37.0	33.0	39.0
7	35.46303817692584	35.0	34.0	37.0	33.0	39.0
8	35.61999704367523	36.0	35.0	38.0	33.0	39.0
9	35.5962130320474	36.0	35.0	38.0	33.0	39.0
10-14	35.51740623836146	36.0	34.8	37.8	33.0	39.0
15-19	35.52377076186471	36.0	35.0	38.0	33.0	39.0
20-24	35.50464134248281	36.0	34.8	38.0	33.0	39.0
25-29	35.483772486873136	36.0	34.2	38.0	33.0	39.0
30-34	35.45712200575855	36.0	34.0	38.0	33.0	39.0
35-39	35.43151582077079	36.0	34.0	38.0	33.0	39.0
40-44	35.41048118285959	36.0	34.0	38.0	33.0	39.0
45-49	35.37329602863848	36.0	34.0	38.0	33.0	39.0
50-54	35.35189612518733	36.0	34.0	38.0	32.2	39.0
55-59	35.315136886537104	36.0	34.0	38.0	32.0	39.0
60-64	35.275983574289896	36.0	34.0	38.0	32.0	39.0
65-69	35.23342375335777	36.0	34.0	37.8	32.0	39.0
70-74	35.199914157519665	36.0	34.0	37.8	32.0	39.0
75-79	35.14857928863466	36.0	34.0	37.0	32.0	39.0
80-84	35.10592818045619	35.8	34.0	37.0	32.0	39.0
85-89	35.057525759300304	35.2	34.0	37.0	32.0	39.0
90-94	34.99627753670628	35.0	34.0	37.0	31.8	39.0
95-99	34.92709626508618	35.0	34.0	37.0	31.0	39.0
100-104	34.86806019097226	35.0	34.0	37.0	31.0	39.0
105-109	34.7973312654323	35.0	34.0	37.0	31.0	39.0
110-114	34.72823504538616	35.0	34.0	37.0	31.0	39.0
115-119	34.65051584786975	35.0	34.0	37.0	30.2	39.0
120-124	34.565875270484774	35.0	34.0	37.0	30.0	39.0
125-129	34.49565721218573	35.0	34.0	37.0	30.0	39.0
130-134	34.39406113398242	35.0	34.0	37.0	30.0	39.0
135-139	34.28805188146011	35.0	34.0	37.0	29.4	39.0
140-144	34.173603882700924	35.0	34.0	37.0	29.0	39.0
145-149	34.07326599500955	35.0	34.0	37.0	28.8	39.0
150	33.99929409524702	35.0	34.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	21.0
21	276.0
22	2124.0
23	10303.0
24	33585.0
25	80111.0
26	154473.0
27	257583.0
28	367113.0
29	475512.0
30	608150.0
31	794564.0
32	1074469.0
33	1535986.0
34	2383774.0
35	4211285.0
36	1.0514288E7
37	1519447.0
38	5.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.13945165461985	18.908456791390243	16.613704384422793	31.338387169567124
2	24.80263346857833	25.078459996994557	33.00309660672012	17.11580992770699
3	20.119746555300942	29.27498736527066	30.43881025868841	20.166455820739987
4	23.77985510510751	33.654850677072105	22.560264885389955	20.005029332430425
5	25.583495365080317	35.17655736756376	22.423749337615885	16.81619792974004
6	18.966784055210926	39.44461555310726	22.556425029922277	19.032175361759535
7	20.396030965399888	20.94485754964467	38.21389446836335	20.44521701659209
8	21.382683395585993	23.583297222211815	28.559984215173166	26.474035167029026
9	23.595130697486596	23.106175725826226	28.997287649027054	24.30140592766012
10-14	23.02769352919492	29.519049732099532	25.702658793547773	21.75059794515777
15-19	22.95376640230552	28.46019894791969	27.147334327072503	21.43870032270229
20-24	23.038058065416017	28.520170701907098	27.065470747395043	21.376300485281842
25-29	23.14677419885243	28.418081934653568	26.97798045886806	21.457163407625945
30-34	23.12271149679406	28.197151812938486	27.201777824325625	21.47835886594183
35-39	23.218294613540458	28.137140334805654	27.175191714664628	21.469373336989264
40-44	23.338831242683337	27.910202654772988	27.30692424794482	21.444041854598854
45-49	23.34586800618346	27.72879227413341	27.395024785923706	21.530314933759424
50-54	23.3943923887898	27.70168689426405	27.347278411611548	21.556642305334606
55-59	23.55781739978207	27.581904911324617	27.288898242859954	21.571379446033355
60-64	23.456614685108327	27.55649766842882	27.34675013032264	21.640137516140214
65-69	23.575833414470615	27.469982501404587	27.303543331325127	21.650640752799674
70-74	23.628351262833966	27.423221618917914	27.21469374169522	21.733733376552898
75-79	23.567608380587284	27.505057575778842	27.27964759009616	21.64768645353771
80-84	23.693690952334943	27.528384693197633	27.127951293154815	21.649973061312604
85-89	23.694280385717544	27.517088885097163	27.182235776599917	21.60639495258537
90-94	23.654844128796025	27.43846196849687	27.22212160135563	21.68457230135148
95-99	23.711897728704873	27.52128275099836	27.255154391346466	21.5116651289503
100-104	23.907512671652494	27.495024949017342	27.06288784800797	21.53457453132219
105-109	23.672814748336126	27.63929770076936	27.179688678099296	21.50819887279522
110-114	23.71519694546963	27.634379467970604	27.0899919498219	21.560431636737867
115-119	23.77407946799406	27.62747286148296	27.135660620098424	21.462787050424552
120-124	23.751188180728434	27.55192789464761	27.27430643650847	21.422577488115486
125-129	23.71168948382994	27.654238710779318	27.25028536110142	21.383786444289324
130-134	23.7761983053707	27.54787512387571	27.336240233398968	21.33968633735462
135-139	23.668381948336247	27.540576306834375	27.43486126315824	21.356180481671142
140-144	23.70797472146671	27.66905446851487	27.398317225969986	21.224653584048433
145-149	23.82758807148345	28.244781027371562	26.86233246365546	21.06529843748953
150	23.436649710605277	28.157032878935418	27.016541723578975	21.389775686880334
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	2.0
2	7.5
3	27.0
4	46.5
5	50.0
6	45.0
7	39.5
8	42.0
9	31.5
10	19.0
11	18.5
12	14.5
13	17.5
14	31.0
15	54.5
16	79.5
17	134.0
18	240.5
19	442.5
20	721.0
21	1156.0
22	1830.0
23	3060.5
24	5155.5
25	8226.5
26	12616.0
27	19665.0
28	31184.5
29	45970.0
30	62605.0
31	83199.0
32	111252.5
33	148451.0
34	195018.5
35	265455.0
36	371128.0
37	495177.5
38	640796.5
39	828262.0
40	1047600.0
41	1248420.0
42	1423243.0
43	1582626.0
44	1691460.0
45	1735268.5
46	1721887.0
47	1659028.5
48	1551515.0
49	1402029.0
50	1217463.5
51	1018687.0
52	828438.5
53	658054.0
54	511658.5
55	386565.0
56	285491.5
57	211737.5
58	155633.5
59	111803.5
60	77589.5
61	52042.5
62	35481.5
63	24382.0
64	16111.0
65	10620.0
66	7403.5
67	5545.5
68	4783.0
69	3443.5
70	1835.0
71	1134.5
72	577.0
73	393.5
74	346.5
75	252.5
76	166.0
77	60.5
78	17.5
79	1.5
80	0.5
81	0.0
82	0.0
83	1.0
84	1.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.162665131364762E-6
3	0.0
4	8.325330262729524E-6
5	4.162665131364762E-6
6	0.0
7	0.0
8	4.162665131364762E-6
9	0.0
10-14	0.10149326869990935
15-19	0.0
20-24	8.325330262729524E-7
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	8.325330262729524E-7
50-54	1.6650660525459047E-6
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	1.6650660525459047E-6
80-84	0.0
85-89	0.0
90-94	0.0
95-99	7.492797236456572E-6
100-104	2.497599078818857E-6
105-109	1.6650660525459047E-6
110-114	0.0
115-119	3.3301321050918095E-6
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4023071E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.733434883139026
#Duplication Level	Percentage of total
1	25.68855866310441
2	11.195199609088844
3	7.369428762205192
4	5.508320329312903
5	4.287620429681981
6	3.220375696573467
7	2.6712653366051162
8	2.356868111267842
9	1.9183213755515696
>10	21.96843169502187
>50	5.505966447656748
>100	6.254282893762494
>500	0.9463352249322049
>1k	0.9936591605452407
>5k	0.07080825237962152
>10k+	0.04455801231069437
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	4.162665131364762E-6	4.162665131364762E-6	0.0	0.001623439401232257	0.0
2	1.6650660525459047E-5	4.162665131364762E-6	4.162665131364762E-6	4.162665131364762E-6	0.005615435262211064	0.0
3	3.746398618228285E-5	4.162665131364762E-6	4.162665131364762E-6	4.162665131364762E-6	0.007243037328574686	0.0
4	7.909063749593048E-5	8.325330262729524E-6	8.325330262729524E-6	4.162665131364762E-6	0.00855843951008595	0.0
5	7.909063749593048E-5	8.325330262729524E-6	8.325330262729524E-6	4.162665131364762E-6	0.010161065585661384	0.0
6	7.909063749593048E-5	8.325330262729524E-6	8.325330262729524E-6	4.162665131364762E-6	0.011780342321762276	0.0
7	9.157863289002475E-5	2.081332565682381E-5	8.325330262729524E-6	4.162665131364762E-6	0.01389913887362694	0.0
8	1.415306144664019E-4	2.081332565682381E-5	8.325330262729524E-6	4.162665131364762E-6	0.019252326232562022	0.0
9	1.4569327959776667E-4	2.9138655919553333E-5	8.325330262729524E-6	4.162665131364762E-6	0.025117521402654972	0.0
10-11	1.7691326808300236E-4	3.746398618228285E-5	1.6650660525459047E-5	4.162665131364762E-6	0.031315729783257105	0.0
12-13	2.1021458913392048E-4	3.746398618228285E-5	1.6650660525459047E-5	4.162665131364762E-6	0.03736616355169578	0.0
14-15	2.2686524965937952E-4	3.746398618228285E-5	1.6650660525459047E-5	4.162665131364762E-6	0.04604115768545995	0.0
16-17	2.6224790327598E-4	3.746398618228285E-5	1.6650660525459047E-5	4.162665131364762E-6	0.05185431954141084	0.0
18-19	3.1219988485235717E-4	3.954531874796524E-5	1.6650660525459047E-5	4.162665131364762E-6	0.05997776054526917	0.0
20-21	3.475825384689576E-4	4.7870649010694756E-5	1.6650660525459047E-5	4.162665131364762E-6	0.06583463038509939	0.0
22-23	3.8712785721692285E-4	7.49279723645657E-5	1.6650660525459047E-5	4.162665131364762E-6	0.07168733755979825	0.0
24-25	4.329171736619352E-4	8.741596775866E-5	1.6650660525459047E-5	4.162665131364762E-6	0.07686569298321601	0.0
26-27	4.641371621471709E-4	9.990396315275428E-5	1.6650660525459047E-5	4.162665131364762E-6	0.08079524886722433	0.0
28-29	5.577971276028781E-4	9.990396315275428E-5	1.6650660525459047E-5	4.162665131364762E-6	0.08482262738181975	0.0
30-31	6.805957489781386E-4	1.0406662828411904E-4	1.8731993091141427E-5	4.162665131364762E-6	0.08987610285129657	0.0
32-33	8.03394370353399E-4	1.0614796084980143E-4	2.081332565682381E-5	4.162665131364762E-6	0.09492333432307634	0.0
34-35	8.970543358091062E-4	1.1239195854684857E-4	2.081332565682381E-5	4.162665131364762E-6	0.09828676774921907	0.0
36-37	9.553316476482129E-4	1.1655462367821333E-4	2.081332565682381E-5	4.162665131364762E-6	0.10183752110627323	4.162665131364762E-6
38-39	0.0010198529571843666	1.2071728880957809E-4	2.081332565682381E-5	4.162665131364762E-6	0.10543406377977238	4.162665131364762E-6
40-41	0.001059398275932332	1.2279862137526046E-4	2.081332565682381E-5	6.243997697047143E-6	0.10830422138784837	4.162665131364762E-6
42-43	0.0010885369318518853	1.2904261907230763E-4	2.081332565682381E-5	8.325330262729524E-6	0.11127012029394577	4.162665131364762E-6
44-45	0.0011218382529028033	1.498559447291314E-4	2.081332565682381E-5	8.325330262729524E-6	0.11363451408856096	4.162665131364762E-6
46-47	0.0011634649042164508	1.977265937398262E-4	2.081332565682381E-5	8.325330262729524E-6	0.1161924718117846	4.162665131364762E-6
48-49	0.0012113355532271455	1.9980792630550857E-4	2.081332565682381E-5	8.325330262729524E-6	0.11853813361330864	4.162665131364762E-6
50-51	0.001244636874278064	2.060519240025557E-4	2.081332565682381E-5	8.325330262729524E-6	0.12047169156682758	4.162665131364762E-6
52-53	0.0012737755301976171	2.1437725426528523E-4	2.081332565682381E-5	8.325330262729524E-6	0.12251764147889335	4.162665131364762E-6
54-55	0.0013029141861171704	2.164585868309676E-4	2.081332565682381E-5	8.325330262729524E-6	0.12428469282715769	4.162665131364762E-6
56-57	0.0013632728305219594	2.2062125196233237E-4	2.081332565682381E-5	8.325330262729524E-6	0.12635353739744598	4.162665131364762E-6
58-59	0.0014402821354522076	2.372719124877914E-4	2.081332565682381E-5	8.325330262729524E-6	0.12857223791246342	4.162665131364762E-6
60-61	0.0015048034449883613	2.6016657071029764E-4	2.081332565682381E-5	8.325330262729524E-6	0.13013531866929084	8.325330262729524E-6
62-63	0.001519372772948138	2.7889856380143905E-4	2.081332565682381E-5	1.0406662828411905E-5	0.13227909121194373	1.4569327959776666E-5
64-65	0.0015360234334735972	2.9763055689258045E-4	2.081332565682381E-5	1.2487995394094285E-5	0.13424803181907924	1.6650660525459047E-5
66-67	0.0015859754150499743	3.2260654768076903E-4	2.081332565682381E-5	1.4569327959776666E-5	0.13571120861275396	1.6650660525459047E-5
68-69	0.0016192767361008923	3.4550120590327524E-4	2.081332565682381E-5	1.6650660525459047E-5	0.1371577347459032	1.6650660525459047E-5
70-71	0.001650496724586128	3.5590786873168715E-4	2.081332565682381E-5	1.6650660525459047E-5	0.1385064382484654	1.8731993091141427E-5
72-73	0.0016942047084654581	3.704771966914638E-4	2.081332565682381E-5	1.6650660525459047E-5	0.13947633922407338	2.2894658222506192E-5
74-75	0.0017170993666879642	3.808838595198757E-4	2.081332565682381E-5	1.6650660525459047E-5	0.14064812945855257	2.9138655919553333E-5
76-77	0.0017399940249104703	4.0585985030806425E-4	2.081332565682381E-5	1.6650660525459047E-5	0.14188027833743655	2.9138655919553333E-5
78-79	0.0017753766785270709	4.4124250392466475E-4	2.081332565682381E-5	1.6650660525459047E-5	0.14335802445907103	3.3301321050918095E-5
80-81	0.001787864673921165	4.495678341873943E-4	2.081332565682381E-5	1.6650660525459047E-5	0.14472754128729004	4.162665131364762E-5
82-83	0.0018149219972750361	4.7662515754126524E-4	2.081332565682381E-5	2.081332565682381E-5	0.1463405740256939	4.162665131364762E-5
84-85	0.0018398979880632246	4.8703182036967716E-4	2.081332565682381E-5	2.2894658222506192E-5	0.14800147741310843	4.370798387933E-5
86-87	0.001862792646285731	5.016011483294538E-4	2.081332565682381E-5	2.497599078818857E-5	0.14968735679131115	4.578931644501238E-5
88-89	0.0018731993091141428	5.099264785921833E-4	2.081332565682381E-5	2.497599078818857E-5	0.1514273508162216	4.578931644501238E-5
90-91	0.0018773619742455075	5.494717973401485E-4	2.081332565682381E-5	2.497599078818857E-5	0.15248674909215396	4.578931644501238E-5
92-93	0.001889849969639602	5.619597927342428E-4	2.497599078818857E-5	2.497599078818857E-5	0.15354198470295494	4.578931644501238E-5
94-95	0.0018981752999023315	5.744477881283372E-4	2.497599078818857E-5	2.497599078818857E-5	0.1546326029673725	4.995198157637714E-5
96-97	0.0019085819627307433	5.84854450956749E-4	2.497599078818857E-5	2.497599078818857E-5	0.1556566185896882	4.995198157637714E-5
98-99	0.0019148259604277905	5.952611137851609E-4	2.497599078818857E-5	2.497599078818857E-5	0.15668479687713532	4.995198157637714E-5
100-101	0.0019252326232562023	5.994237789165256E-4	2.497599078818857E-5	2.497599078818857E-5	0.15752357390110533	4.995198157637714E-5
102-103	0.0019273139558218847	6.035864440478904E-4	2.497599078818857E-5	2.497599078818857E-5	0.1585038815395417	4.995198157637714E-5
104-105	0.001939801951215979	6.077491091792552E-4	2.497599078818857E-5	2.9138655919553333E-5	0.1595070838362006	4.995198157637714E-5
106-107	0.0019606152768728027	6.1191177431062E-4	2.497599078818857E-5	2.9138655919553333E-5	0.16038956884404998	4.995198157637714E-5
108-109	0.001968940607135532	6.181557720076671E-4	2.497599078818857E-5	2.9138655919553333E-5	0.161280379182162	4.995198157637714E-5
110-111	0.001981428602529627	6.285624348360791E-4	3.3301321050918095E-5	2.9138655919553333E-5	0.16220865350645636	4.995198157637714E-5
112-113	0.001993916597923721	6.389690976644909E-4	4.162665131364762E-5	2.9138655919553333E-5	0.1630744878537802	4.995198157637714E-5
114-115	0.0020022419281864505	6.577010907556324E-4	4.578931644501238E-5	2.9138655919553333E-5	0.16387996355669932	5.8277311839106666E-5
116-117	0.00201056725844918	6.618637558869971E-4	4.578931644501238E-5	2.9138655919553333E-5	0.1648498645323073	6.243997697047143E-5
118-119	0.0020147299235805448	6.660264210183619E-4	4.578931644501238E-5	2.9138655919553333E-5	0.1656990482191057	6.868397466751856E-5
120-121	0.0020147299235805448	6.701890861497266E-4	4.578931644501238E-5	2.9138655919553333E-5	0.16666686786214802	7.284663979888333E-5
122-123	0.002016811256146227	6.764330838467738E-4	4.578931644501238E-5	2.9138655919553333E-5	0.16744528624171323	7.909063749593048E-5
124-125	0.002020973921277592	6.805957489781386E-4	4.995198157637714E-5	2.9138655919553333E-5	0.16840894321962419	8.325330262729524E-5
126-127	0.0020292992515403216	6.82677081543821E-4	4.995198157637714E-5	2.9138655919553333E-5	0.16949956148404174	9.157863289002475E-5
128-129	0.0020397059143687332	6.910024118065505E-4	4.995198157637714E-5	2.9138655919553333E-5	0.17052357710635746	9.574129802138953E-5
130-131	0.0020417872469344154	6.9932774206928E-4	4.995198157637714E-5	2.9138655919553333E-5	0.17161419537077505	9.990396315275428E-5
132-133	0.0020584379074598744	7.034904072006447E-4	4.995198157637714E-5	2.9138655919553333E-5	0.17236347509442068	1.0198529571843666E-4
134-135	0.0021000645587735227	7.034904072006447E-4	4.995198157637714E-5	3.1219988485235715E-5	0.17319808945325932	1.082292934154838E-4
136-137	0.0021292032146930755	7.055717397663271E-4	4.995198157637714E-5	3.5382653616600474E-5	0.17419921041735253	1.1239195854684857E-4
138-139	0.0021437725426528523	7.180597351604214E-4	4.995198157637714E-5	3.746398618228285E-5	0.175094183420596	1.1239195854684857E-4
>>END_MODULE
