##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.4d.S.1.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23132287
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.066859320913665	37.0	35.0	38.0	33.0	39.0
2	35.55006688270814	35.0	34.0	38.0	33.0	39.0
3	35.660114670028086	36.0	35.0	38.0	33.0	39.0
4	35.763383231411574	36.0	35.0	38.0	33.0	39.0
5	35.701811411902334	36.0	35.0	38.0	33.0	39.0
6	35.625968889284486	36.0	35.0	37.0	33.0	39.0
7	35.59154708741077	35.0	34.0	38.0	33.0	39.0
8	35.742133754435955	36.0	35.0	38.0	33.0	39.0
9	35.735314843707414	36.0	35.0	38.0	33.0	39.0
10-14	35.65459209459056	36.0	35.0	37.8	33.0	39.0
15-19	35.66128625327881	36.0	35.0	38.0	33.0	39.0
20-24	35.64573265929132	36.0	35.0	38.0	33.0	39.0
25-29	35.63352464025714	36.0	35.0	38.0	33.0	39.0
30-34	35.6084732650948	36.0	35.0	38.0	33.0	39.0
35-39	35.585181274985906	36.0	35.0	38.0	33.0	39.0
40-44	35.56979753882528	36.0	35.0	38.0	33.0	39.0
45-49	35.53863860499396	36.0	35.0	38.0	33.0	39.0
50-54	35.52099457351537	36.0	34.8	38.0	33.0	39.0
55-59	35.48814141031537	36.0	34.4	38.0	33.0	39.0
60-64	35.450539741271584	36.0	34.0	38.0	33.0	39.0
65-69	35.41505576167199	36.0	34.0	38.0	32.8	39.0
70-74	35.38542564338753	36.0	34.0	38.0	32.0	39.0
75-79	35.33653507757361	36.0	34.0	38.0	32.0	39.0
80-84	35.2966234337314	36.0	34.0	38.0	32.0	39.0
85-89	35.25415064234677	36.0	34.0	38.0	32.0	39.0
90-94	35.20096625119687	36.0	34.0	38.0	32.0	39.0
95-99	35.1366929780873	36.0	34.0	38.0	32.0	39.0
100-104	35.086709792248385	36.0	34.0	38.0	32.0	39.0
105-109	35.02368381474776	36.0	34.0	38.0	31.6	39.0
110-114	34.95143995057644	35.8	34.0	37.6	31.0	39.0
115-119	34.88057554361141	35.0	34.0	37.0	31.0	39.0
120-124	34.80315007331528	35.0	34.0	37.0	31.0	39.0
125-129	34.74107437798951	35.0	34.0	37.0	30.8	39.0
130-134	34.64712003616417	35.0	34.0	37.0	30.0	39.0
135-139	34.55594578261976	35.0	34.0	37.0	30.0	39.0
140-144	34.46043723216819	35.0	34.0	37.0	30.0	39.0
145-149	34.36831151195729	35.0	34.0	37.0	29.8	39.0
150	34.30919791890875	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	18.0
21	227.0
22	1810.0
23	8867.0
24	28863.0
25	68103.0
26	129372.0
27	216321.0
28	311375.0
29	404187.0
30	518007.0
31	679898.0
32	922391.0
33	1323344.0
34	2073973.0
35	3752381.0
36	1.0536315E7
37	2156829.0
38	6.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.08683802370418	19.207535303687667	15.55715245782453	32.14847421478361
2	24.65681947733138	25.828679448282806	31.987997208749707	17.526503865636105
3	20.313971563381156	29.5009797129432	29.926514828668466	20.258533895007176
4	23.711685909468695	33.98841342355875	21.893435002489596	20.406465664482965
5	25.77873082760905	35.06809335367489	21.77466067233214	17.378515146383926
6	19.352868972828713	39.52884725703288	21.770952511999894	19.347331258138517
7	20.61582583684873	21.21612964597923	36.929889379290515	21.238155137881527
8	21.636719274665754	23.9751607785257	28.00287321353051	26.385246733278038
9	23.80538941091298	23.591670810586088	27.785086705867002	24.817853072633934
10-14	23.161042775905692	29.32872510302438	25.160908235141843	22.34932388592809
15-19	23.312132518501087	28.060960855275574	26.670392771799868	21.956513854423473
20-24	23.181718262444175	28.114240498572407	26.61120277471916	22.092838464264254
25-29	23.327431481374926	27.7177107477527	26.570059415223408	22.384798355648968
30-34	23.16167029366072	27.431652801399643	26.78988313425199	22.616793770687643
35-39	23.466982953021315	27.154882931850903	26.795674174331957	22.582459940795825
40-44	23.625736616530826	26.831229441343176	26.873665366506994	22.669368575619004
45-49	23.548216599147473	26.649834723646947	26.977163364583568	22.82478531262201
50-54	23.678691582526042	26.64497162161219	26.770146449767747	22.906190346094018
55-59	23.79070603784226	26.563426262176325	26.82169817450389	22.82416952547753
60-64	23.519076172624004	26.507328047589933	26.90432208453924	23.06927369524682
65-69	23.674392419564914	26.469362065238077	26.859102171782666	22.997143343414336
70-74	23.867226996338296	26.360887069755677	26.678955061200433	23.09293087270559
75-79	23.722456181893786	26.493584715541395	26.87045363798619	22.913505464578627
80-84	23.988236009695022	26.481532932735963	26.579429003280136	22.950802054288882
85-89	23.81053546499747	26.589551651334776	26.738137046285132	22.861775837382616
90-94	23.7883603986065	26.392813646138833	26.72831181802301	23.090514137231654
95-99	23.788970964650876	26.60088847250116	26.78561297400525	22.82452758884271
100-104	24.021912092149922	26.332605386856955	26.746478865202377	22.899003655790747
105-109	23.827605113147694	26.54043156217109	26.701609745720344	22.93035357896087
110-114	23.925720790166576	26.631114338154287	26.600621892681858	22.842542978997276
115-119	24.02089425917982	26.65384187910171	26.744505634051663	22.580758227666813
120-124	23.931065907413878	26.75795546508614	26.924469914491983	22.386508713008002
125-129	23.950419601831847	26.860417216853655	26.761443864154028	22.42771931716047
130-134	24.13305956302548	26.848857616196792	26.70131319052025	22.316769630257483
135-139	23.962854170017863	26.877905327735213	26.917967946705833	22.24127255554109
140-144	23.952693479896737	27.02340845070788	26.769828681444253	22.254069387951137
145-149	24.09867299329288	27.694512868528737	26.180588196921473	22.02622594125691
150	23.887037196106032	27.664761378760343	26.151824071696844	22.296377353436778
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	8.5
3	28.0
4	40.5
5	45.0
6	43.5
7	35.5
8	26.0
9	24.0
10	19.5
11	13.0
12	15.0
13	14.5
14	26.0
15	46.0
16	66.0
17	109.0
18	212.0
19	364.0
20	590.0
21	917.5
22	1398.5
23	2207.5
24	3579.0
25	5643.0
26	8707.0
27	13362.5
28	19713.5
29	27656.0
30	38160.0
31	54641.0
32	79950.0
33	109043.0
34	144545.5
35	202765.5
36	285771.0
37	379950.5
38	491158.0
39	636890.0
40	811157.5
41	993333.0
42	1176959.5
43	1345055.0
44	1472642.0
45	1550045.0
46	1572326.5
47	1530362.0
48	1465707.5
49	1402979.0
50	1286993.0
51	1123555.0
52	959835.5
53	802753.0
54	658939.5
55	548668.0
56	451548.5
57	372671.0
58	314191.5
59	257078.0
60	196764.0
61	134510.0
62	82489.0
63	48798.5
64	30089.5
65	15518.5
66	8442.0
67	4467.5
68	2328.0
69	1382.0
70	878.5
71	678.0
72	434.0
73	320.5
74	261.5
75	160.0
76	111.5
77	48.5
78	12.0
79	3.0
80	2.5
81	1.5
82	0.0
83	0.0
84	0.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.322962100548035E-6
2	4.322962100548035E-6
3	4.322962100548035E-6
4	4.322962100548035E-6
5	0.0
6	4.322962100548035E-6
7	0.0
8	0.0
9	0.0
10-14	0.09753640009740498
15-19	0.0
20-24	0.0
25-29	0.0
30-34	8.64592420109607E-7
35-39	8.64592420109607E-7
40-44	0.0
45-49	8.64592420109607E-7
50-54	1.729184840219214E-6
55-59	0.0
60-64	0.0
65-69	0.0
70-74	8.64592420109607E-7
75-79	8.64592420109607E-7
80-84	0.0
85-89	0.0
90-94	0.0
95-99	4.322962100548035E-6
100-104	8.64592420109607E-7
105-109	0.0
110-114	0.0
115-119	0.0
120-124	8.64592420109607E-7
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.3132287E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.918422663367146
#Duplication Level	Percentage of total
1	22.12682550924761
2	9.484980657643524
3	6.072593171832403
4	4.279743227447422
5	3.2845091806945823
6	2.549740445608772
7	2.0948172988898524
8	1.758419071538646
9	1.5318782554830783
>10	19.103626847183584
>50	6.408323744310676
>100	12.673813228324281
>500	3.5588173532319267
>1k	4.277279157648064
>5k	0.47192127118038246
>10k+	0.32271157973495546
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.4151400594329475E-4	0.0	4.322962100548035E-6	0.0	0.002956906076774856	0.0
2	0.0018977803621405872	8.64592420109607E-6	4.322962100548035E-6	0.0	0.013686498010335078	0.0
3	0.002710497237043618	8.64592420109607E-6	4.322962100548035E-6	0.0	0.018044043807687496	0.0
4	0.0064757972266209565	8.64592420109607E-6	4.322962100548035E-6	0.0	0.02448093437540352	0.0
5	0.006661684596944522	1.2968886301644105E-5	4.322962100548035E-6	0.0	0.029288068231212935	0.0
6	0.00787643694719852	1.2968886301644105E-5	4.322962100548035E-6	0.0	0.036978617808087894	0.0
7	0.010042240959573085	1.729184840219214E-5	4.322962100548035E-6	0.0	0.047487738674520164	0.0
8	0.01777602015745352	2.1614810502740176E-5	4.322962100548035E-6	0.0	0.06424786273834489	0.0
9	0.018359620041027504	2.593777260328821E-5	4.322962100548035E-6	0.0	0.07681903652673858	0.0
10-11	0.018999418431908614	2.593777260328821E-5	6.484443150822052E-6	0.0	0.13071340503427092	0.0
12-13	0.0197408064321526	3.242221575411026E-5	8.64592420109607E-6	0.0	0.15980261700885867	0.0
14-15	0.02227406222307375	3.458369680438428E-5	8.64592420109607E-6	0.0	0.17270665887899456	0.0
16-17	0.02499536686536874	3.458369680438428E-5	8.64592420109607E-6	0.0	0.18077546763966745	0.0
18-19	0.031255015986962294	3.8906658904932314E-5	8.64592420109607E-6	0.0	0.1903205679576775	0.0
20-21	0.03708236889850104	3.8906658904932314E-5	8.64592420109607E-6	0.0	0.19630570898588626	0.0
22-23	0.03957023358736644	5.187554520657642E-5	8.64592420109607E-6	0.0	0.2025178055243738	0.0
24-25	0.04459351554820325	5.187554520657642E-5	8.64592420109607E-6	0.0	0.20872125613866022	0.0
26-27	0.04877814286153375	5.187554520657642E-5	8.64592420109607E-6	0.0	0.2139823010150272	0.0
28-29	0.05867556459073848	5.187554520657642E-5	8.64592420109607E-6	0.0	0.21899045260851208	0.0
30-31	0.08113335270308553	5.6198507307124456E-5	1.2968886301644105E-5	4.322962100548035E-6	0.22397482791044396	0.0
32-33	0.10273735580057432	7.132887465904257E-5	1.2968886301644105E-5	4.322962100548035E-6	0.2281616167048247	0.0
34-35	0.1197265968557281	7.781331780986463E-5	1.2968886301644105E-5	4.322962100548035E-6	0.23238731215811043	0.0
36-37	0.12274834736401118	8.213627991041267E-5	1.2968886301644105E-5	4.322962100548035E-6	0.23509132495200324	4.322962100548035E-6
38-39	0.12535941647274218	8.64592420109607E-5	1.2968886301644105E-5	4.322962100548035E-6	0.23706691863195367	4.322962100548035E-6
40-41	0.12693945912049248	8.862072306123472E-5	1.2968886301644105E-5	4.322962100548035E-6	0.2388371716121281	4.322962100548035E-6
42-43	0.12724422794858115	9.294368516178275E-5	1.2968886301644105E-5	4.322962100548035E-6	0.24057932533864895	4.322962100548035E-6
44-45	0.12810017444448962	9.510516621205676E-5	1.2968886301644105E-5	4.322962100548035E-6	0.24201887171813147	4.322962100548035E-6
46-47	0.12940787047990543	9.510516621205676E-5	1.2968886301644105E-5	4.322962100548035E-6	0.2434368032871112	4.322962100548035E-6
48-49	0.130564262841802	9.726664726233078E-5	1.2968886301644105E-5	4.322962100548035E-6	0.2448006978298341	4.322962100548035E-6
50-51	0.13147208488291712	9.94281283126048E-5	1.2968886301644105E-5	4.322962100548035E-6	0.24635696418603142	8.64592420109607E-6
52-53	0.1322977706441218	9.94281283126048E-5	1.2968886301644105E-5	4.322962100548035E-6	0.24764736837304502	8.64592420109607E-6
54-55	0.13271709796787495	9.94281283126048E-5	1.2968886301644105E-5	4.322962100548035E-6	0.24882969850754488	8.64592420109607E-6
56-57	0.13367031111104577	9.94281283126048E-5	1.2968886301644105E-5	4.322962100548035E-6	0.2500855189977541	8.64592420109607E-6
58-59	0.13387132884872127	1.0375109041315284E-4	1.2968886301644105E-5	4.322962100548035E-6	0.2511489676744889	8.64592420109607E-6
60-61	0.1342106813736143	1.0591257146342686E-4	1.2968886301644105E-5	4.322962100548035E-6	0.25211514970396137	8.64592420109607E-6
62-63	0.13449167391014993	1.0807405251370088E-4	1.2968886301644105E-5	4.322962100548035E-6	0.25320885911540003	8.64592420109607E-6
64-65	0.13452841908800456	1.102355335639749E-4	1.2968886301644105E-5	4.322962100548035E-6	0.2543674129583469	8.64592420109607E-6
66-67	0.134610555367915	1.1455849566452292E-4	1.2968886301644105E-5	4.322962100548035E-6	0.25519742168165216	8.64592420109607E-6
68-69	0.13465378498892047	1.1888145776507096E-4	1.2968886301644105E-5	4.322962100548035E-6	0.25604256077230925	8.64592420109607E-6
70-71	0.13474456719303196	1.2104293881534497E-4	1.2968886301644105E-5	4.322962100548035E-6	0.25697415910497734	1.2968886301644105E-5
72-73	0.13479644273823854	1.2536590091589302E-4	1.2968886301644105E-5	4.322962100548035E-6	0.2576485411926629	1.2968886301644105E-5
74-75	0.1348245419918921	1.2752738196616703E-4	1.2968886301644105E-5	4.322962100548035E-6	0.25846774251071675	1.2968886301644105E-5
76-77	0.1348591256886965	1.361733061672631E-4	1.2968886301644105E-5	4.322962100548035E-6	0.25918751570045795	1.2968886301644105E-5
78-79	0.134926131601255	1.4698071141863318E-4	1.2968886301644105E-5	4.322962100548035E-6	0.2600974992226234	1.5130367351918122E-5
80-81	0.1349671997412102	1.5778811667000326E-4	1.2968886301644105E-5	4.322962100548035E-6	0.2610182901500401	3.0260734703836243E-5
82-83	0.1349996219569643	1.6427255982082533E-4	1.2968886301644105E-5	4.322962100548035E-6	0.2618872055322502	3.0260734703836243E-5
84-85	0.1350320441727184	1.6427255982082533E-4	1.2968886301644105E-5	4.322962100548035E-6	0.26277125128181233	3.0260734703836243E-5
86-87	0.13504717454007034	1.6427255982082533E-4	1.5130367351918122E-5	4.322962100548035E-6	0.26360558296721803	3.0260734703836243E-5
88-89	0.13507743527477417	1.6427255982082533E-4	1.729184840219214E-5	4.322962100548035E-6	0.26445504501997574	3.0260734703836243E-5
90-91	0.13509905008527692	1.6427255982082533E-4	1.729184840219214E-5	4.322962100548035E-6	0.2651142967403093	3.242221575411026E-5
92-93	0.13511850341472936	1.6427255982082533E-4	1.729184840219214E-5	8.64592420109607E-6	0.26569357366178276	3.458369680438428E-5
94-95	0.13516389451678512	1.6427255982082533E-4	1.9453329452466157E-5	1.2968886301644105E-5	0.26645441499147926	3.6745177854658296E-5
96-97	0.13517037895993594	1.6427255982082533E-4	2.1614810502740176E-5	1.2968886301644105E-5	0.26714825040861717	3.8906658904932314E-5
98-99	0.1351833478462376	1.6427255982082533E-4	2.1614810502740176E-5	1.2968886301644105E-5	0.2678356013826043	3.8906658904932314E-5
100-101	0.1352006396946398	1.6427255982082533E-4	2.1614810502740176E-5	1.2968886301644105E-5	0.26845378496298267	3.8906658904932314E-5
102-103	0.13522009302409227	1.6427255982082533E-4	2.1614810502740176E-5	1.2968886301644105E-5	0.269087098910713	3.8906658904932314E-5
104-105	0.13523738487249443	1.6427255982082533E-4	2.1614810502740176E-5	1.2968886301644105E-5	0.26974418914999626	4.106813995520633E-5
106-107	0.13525251523984635	1.6427255982082533E-4	2.1614810502740176E-5	1.2968886301644105E-5	0.27025429867786094	4.322962100548035E-5
108-109	0.13528493745560047	1.6859552192137337E-4	2.593777260328821E-5	1.2968886301644105E-5	0.27085086744773657	4.5391102055754363E-5
110-111	0.1353151981903043	1.6859552192137337E-4	2.593777260328821E-5	1.2968886301644105E-5	0.2714841813954669	4.755258310602838E-5
112-113	0.1353260055955557	1.707570029716474E-4	2.593777260328821E-5	1.2968886301644105E-5	0.27210452645689553	4.97140641563024E-5
114-115	0.13533897448185733	1.729184840219214E-4	2.593777260328821E-5	1.2968886301644105E-5	0.27269893374572085	5.187554520657642E-5
116-117	0.1353497818871087	1.729184840219214E-4	2.593777260328821E-5	1.2968886301644105E-5	0.27327821066719427	5.187554520657642E-5
118-119	0.1353584278113098	1.729184840219214E-4	3.0260734703836243E-5	1.9453329452466157E-5	0.2738510031455169	5.8359988357398475E-5
120-121	0.13537139669761145	1.729184840219214E-4	3.0260734703836243E-5	2.1614810502740176E-5	0.2744410874722417	6.26829504579465E-5
122-123	0.13539301150811417	1.729184840219214E-4	3.0260734703836243E-5	2.1614810502740176E-5	0.27506575549577095	6.916739360876855E-5
124-125	0.1354081418754661	1.729184840219214E-4	3.0260734703836243E-5	2.1614810502740176E-5	0.27575310646975804	7.349035570931659E-5
126-127	0.1354167877996672	1.7724144612246944E-4	3.0260734703836243E-5	2.1614810502740176E-5	0.276509624837354	7.349035570931659E-5
128-129	0.13542543372386828	1.858873703235655E-4	3.0260734703836243E-5	2.1614810502740176E-5	0.2772639817238996	7.781331780986463E-5
130-131	0.13543840261016993	1.9237181347438756E-4	3.0260734703836243E-5	2.1614810502740176E-5	0.2779837549136408	8.213627991041267E-5
132-133	0.1354578559396224	1.9669477557493557E-4	3.0260734703836243E-5	2.1614810502740176E-5	0.27865813700132636	8.429776096068668E-5
134-135	0.13547298630697432	2.0317921872575764E-4	3.0260734703836243E-5	2.1614810502740176E-5	0.2793346805700621	8.64592420109607E-5
136-137	0.1354816322311754	2.0750218082630568E-4	3.0260734703836243E-5	2.1614810502740176E-5	0.2799896093282951	9.078220411150873E-5
138-139	0.13548595519327597	2.1830958607767578E-4	3.0260734703836243E-5	2.1614810502740176E-5	0.2805667247687183	9.510516621205676E-5
>>END_MODULE
