##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.4d.S.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24035536
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.014808739859184	37.0	35.0	38.0	33.0	39.0
2	35.516845474134634	35.0	34.0	38.0	33.0	39.0
3	35.61514288676566	36.0	35.0	38.0	33.0	39.0
4	35.7184576204167	36.0	35.0	38.0	33.0	39.0
5	35.65234937968515	36.0	35.0	38.0	33.0	39.0
6	35.57832344575132	36.0	35.0	37.0	33.0	39.0
7	35.54559619556643	35.0	34.0	38.0	33.0	39.0
8	35.69625416300265	36.0	35.0	38.0	33.0	39.0
9	35.688308136752184	36.0	35.0	38.0	33.0	39.0
10-14	35.598806508829256	36.0	35.0	37.8	33.0	39.0
15-19	35.607121921474935	36.0	35.0	38.0	33.0	39.0
20-24	35.59402892450578	36.0	35.0	38.0	33.0	39.0
25-29	35.57277615943327	36.0	35.0	38.0	33.0	39.0
30-34	35.54771381840621	36.0	35.0	38.0	33.0	39.0
35-39	35.52293296059634	36.0	35.0	38.0	33.0	39.0
40-44	35.50361637036095	36.0	35.0	38.0	33.0	39.0
45-49	35.46406922649863	36.0	34.0	38.0	33.0	39.0
50-54	35.44767880358482	36.0	34.0	38.0	33.0	39.0
55-59	35.40882696354265	36.0	34.0	38.0	32.4	39.0
60-64	35.37062308075842	36.0	34.0	38.0	32.0	39.0
65-69	35.32937036228358	36.0	34.0	38.0	32.0	39.0
70-74	35.29534323678074	36.0	34.0	38.0	32.0	39.0
75-79	35.24320234838949	36.0	34.0	38.0	32.0	39.0
80-84	35.200236657921835	36.0	34.0	38.0	32.0	39.0
85-89	35.1527673275104	36.0	34.0	38.0	32.0	39.0
90-94	35.09475853586124	36.0	34.0	38.0	32.0	39.0
95-99	35.02580529928686	35.8	34.0	38.0	31.4	39.0
100-104	34.9689730572266	35.8	34.0	38.0	31.0	39.0
105-109	34.899766628878176	35.0	34.0	37.0	31.0	39.0
110-114	34.8244572785895	35.0	34.0	37.0	31.0	39.0
115-119	34.74342749002976	35.0	34.0	37.0	30.8	39.0
120-124	34.66087477308598	35.0	34.0	37.0	30.0	39.0
125-129	34.59352408866605	35.0	34.0	37.0	30.0	39.0
130-134	34.492890094067384	35.0	34.0	37.0	30.0	39.0
135-139	34.38976225868231	35.0	34.0	37.0	29.8	39.0
140-144	34.282952083947706	35.0	34.0	37.0	29.0	39.0
145-149	34.1814761443223	35.0	34.0	37.0	29.0	39.0
150	34.11110657153641	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	17.0
21	242.0
22	1834.0
23	8888.0
24	30514.0
25	73479.0
26	144987.0
27	245647.0
28	355312.0
29	458222.0
30	583511.0
31	762913.0
32	1027352.0
33	1460761.0
34	2261397.0
35	4002138.0
36	1.0516303E7
37	2102017.0
38	2.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.10454010772635	18.957854519627627	16.06484167127408	31.87276370137194
2	25.012982861125533	25.059726153785473	32.086222839900294	17.8410681451887
3	20.496577255301368	28.95249471251628	30.274146175652007	20.27678185653034
4	23.900296952004478	33.481378029712175	22.036364991932363	20.581960026350984
5	26.246836298575115	34.71203238971235	21.777594863404943	17.263536448307594
6	19.484334340799986	39.31233484089287	21.7862760283888	19.417054789918346
7	20.839689200190918	20.965540356578693	37.22778223044412	20.966988212786266
8	21.706174699509482	23.611133416049753	28.255078501688374	26.427613382752384
9	23.933409428997525	23.070811612889	28.09342084542741	24.90235811268607
10-14	23.259307402551983	29.056574935571337	25.341616683584633	22.342500978292044
15-19	23.434450556875454	27.88689630220853	26.728703699389104	21.94994944152691
20-24	23.27595645507479	27.999812078246343	26.644258345547335	22.07997312113153
25-29	23.44676501865209	27.614787767701856	26.606043872756103	22.33240334088995
30-34	23.274272920514633	27.36015229583524	26.832390397554605	22.533184386095524
35-39	23.58610517360628	27.10434749614071	26.8111091843344	22.49843814591861
40-44	23.754365203255713	26.83895129278582	26.862575479906088	22.544108024052388
45-49	23.663390739445127	26.67658170801766	26.983840926201935	22.676186626335273
50-54	23.794137741570758	26.653185349888233	26.801843573661355	22.750833334879655
55-59	23.90297266514048	26.59913637873522	26.82513757962377	22.67275337650053
60-64	23.646715430019952	26.5391285636401	26.924730948375768	22.889425057964175
65-69	23.783678664654524	26.503236095947567	26.89219185203269	22.820893387365217
70-74	23.958595306549434	26.43945697736884	26.697047238721865	22.904900477359856
75-79	23.824820010664876	26.528307969609934	26.911746071708027	22.735125948017167
80-84	24.09635216789008	26.551188207327687	26.575382383817026	22.77707724096521
85-89	23.912336300717403	26.66763329097383	26.767140953295154	22.65288945501361
90-94	23.887546339719655	26.457341329937474	26.759830943649437	22.895281386693437
95-99	23.87759243358265	26.658341991971902	26.825906400298045	22.638159174147404
100-104	24.140764741006816	26.397942612971057	26.76682974742065	22.69446289860147
105-109	23.91239870831256	26.60115256010933	26.76406384280342	22.722384888774688
110-114	23.99913278405774	26.693586529545254	26.64784925120871	22.6594314351883
115-119	24.09758617407159	26.699214862526887	26.803517092358582	22.399681871042944
120-124	24.000872707810636	26.794048612021797	26.983211857642786	22.22186682252478
125-129	24.036901028543735	26.878859701734964	26.81296893066999	22.27127033905131
130-134	24.195845684489832	26.87048626666782	26.77077307533312	22.16289497350922
135-139	24.038053488800916	26.909527626094963	26.983783511214394	22.068635373889727
140-144	24.030360712571586	27.050829238840358	26.837907837794837	22.08090221079322
145-149	24.14832561501147	27.73740311407914	26.24433549132144	21.869935779587955
150	23.930005139057435	27.667774914609765	26.2338147982221	22.168405148110697
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	12.5
3	31.5
4	45.0
5	51.5
6	49.0
7	42.0
8	32.5
9	32.0
10	29.0
11	19.0
12	21.5
13	24.0
14	27.5
15	45.0
16	71.0
17	114.0
18	201.0
19	373.0
20	603.5
21	932.0
22	1405.5
23	2195.0
24	3638.5
25	5840.0
26	9043.5
27	13606.0
28	19938.0
29	28178.5
30	39187.0
31	55391.0
32	80690.0
33	110056.0
34	145315.0
35	204619.0
36	289565.5
37	386397.5
38	501667.5
39	656215.0
40	842291.5
41	1031580.0
42	1225907.5
43	1407637.0
44	1543025.5
45	1624736.0
46	1646162.0
47	1603056.5
48	1536907.5
49	1467285.5
50	1342724.0
51	1174599.0
52	1001829.0
53	831191.0
54	679107.5
55	561588.5
56	458895.5
57	379385.0
58	321315.0
59	262816.0
60	200406.5
61	136794.5
62	84610.5
63	50585.5
64	30806.5
65	15492.0
66	8424.5
67	4403.0
68	2152.5
69	1262.5
70	839.5
71	657.0
72	418.5
73	317.5
74	267.5
75	172.0
76	114.5
77	40.5
78	14.0
79	2.5
80	4.0
81	2.5
82	1.0
83	1.0
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.080253171803616E-5
2	8.321012687214466E-6
3	4.160506343607233E-6
4	8.321012687214466E-6
5	1.2481519030821697E-5
6	4.160506343607233E-6
7	0.0
8	8.321012687214466E-6
9	4.160506343607233E-6
10-14	0.10961103592613869
15-19	0.0
20-24	1.6642025374428929E-6
25-29	8.321012687214464E-7
30-34	8.321012687214464E-7
35-39	0.0
40-44	0.0
45-49	0.0
50-54	2.4963038061643394E-6
55-59	0.0
60-64	0.0
65-69	8.321012687214464E-7
70-74	0.0
75-79	3.3284050748857857E-6
80-84	0.0
85-89	0.0
90-94	0.0
95-99	9.15311395593591E-6
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	1.6642025374428929E-6
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4035536E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.046887095194705
#Duplication Level	Percentage of total
1	22.4518443815623
2	9.161994957669222
3	5.919827376644544
4	4.330522098196194
5	3.194835952556579
6	2.480435502987172
7	2.0891430382370504
8	1.8079761755482542
9	1.5517425615173264
>10	19.35744599762088
>50	6.653997398120221
>100	12.856595921211092
>500	3.5034889845443873
>1k	3.9291034303037313
>5k	0.46975214586500524
>10k+	0.2412940774160482
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.2882784889839778E-4	0.0	0.0	0.0	0.0028749098834325976	0.0
2	0.0013729670933903867	0.0	0.0	0.0	0.010663377758665335	0.0
3	0.0020386481083675438	0.0	4.160506343607232E-6	0.0	0.014158203087295412	4.160506343607232E-6
4	0.005213114448539862	0.0	4.160506343607232E-6	0.0	0.019679195005262207	4.160506343607232E-6
5	0.005412818753033009	0.0	4.160506343607232E-6	0.0	0.023739849196622868	4.160506343607232E-6
6	0.006427982300873174	4.160506343607232E-6	4.160506343607232E-6	0.0	0.030579721625513156	4.160506343607232E-6
7	0.008108826863690495	8.321012687214464E-6	4.160506343607232E-6	0.0	0.03973699608779267	4.160506343607232E-6
8	0.01439951245522463	8.321012687214464E-6	4.160506343607232E-6	0.0	0.05474810297552757	4.160506343607232E-6
9	0.014981983343329643	8.321012687214464E-6	4.160506343607232E-6	0.0	0.06584833390027166	4.160506343607232E-6
10-11	0.01554365169971662	1.2481519030821697E-5	4.160506343607232E-6	2.080253171803616E-6	0.1110834391211413	4.160506343607232E-6
12-13	0.016128202840993436	1.2481519030821697E-5	4.160506343607232E-6	4.160506343607232E-6	0.13425329894868998	4.160506343607232E-6
14-15	0.018607864621783345	1.2481519030821697E-5	4.160506343607232E-6	4.160506343607232E-6	0.14450686683251002	8.321012687214464E-6
16-17	0.0214016446315156	1.4561772202625313E-5	4.160506343607232E-6	4.160506343607232E-6	0.15148403597073934	8.321012687214464E-6
18-19	0.02685398819481288	1.8722278546232545E-5	4.160506343607232E-6	4.160506343607232E-6	0.16023358081134534	8.321012687214464E-6
20-21	0.03205046061797831	2.4963038061643394E-5	4.160506343607232E-6	4.160506343607232E-6	0.16593971526160267	8.321012687214464E-6
22-23	0.03419312138493604	2.9123544405250625E-5	4.160506343607232E-6	4.160506343607232E-6	0.17133173148291764	8.321012687214464E-6
24-25	0.03843475760224361	2.9123544405250625E-5	4.160506343607232E-6	4.160506343607232E-6	0.17739774973189698	1.2481519030821697E-5
26-27	0.04222913938761341	2.9123544405250625E-5	4.160506343607232E-6	1.040126585901808E-5	0.18218233202704529	1.2481519030821697E-5
28-29	0.05241197866359211	2.9123544405250625E-5	4.160506343607232E-6	1.2481519030821697E-5	0.18634491862382432	1.6642025374428928E-5
30-31	0.07379906152290508	2.9123544405250625E-5	4.160506343607232E-6	1.6642025374428928E-5	0.19072801205681456	1.8722278546232545E-5
32-33	0.09183901702878605	4.576556977967955E-5	4.160506343607232E-6	1.6642025374428928E-5	0.19435181308209643	2.0802531718036163E-5
34-35	0.105620694291985	4.992607612328679E-5	4.160506343607232E-6	1.6642025374428928E-5	0.19779879258777505	2.0802531718036163E-5
36-37	0.10800674468004375	5.20063292950904E-5	4.160506343607232E-6	1.6642025374428928E-5	0.20003714500063574	2.4963038061643394E-5
38-39	0.10996218266153915	5.8247088810501244E-5	4.160506343607232E-6	1.6642025374428928E-5	0.20182616272838683	2.4963038061643394E-5
40-41	0.11126858165343181	6.240759515410848E-5	4.160506343607232E-6	1.6642025374428928E-5	0.20349660602534514	2.4963038061643394E-5
42-43	0.11149324899598662	6.240759515410848E-5	4.160506343607232E-6	1.6642025374428928E-5	0.20515456780327262	2.4963038061643394E-5
44-45	0.11214020773241754	6.656810149771571E-5	4.160506343607232E-6	1.6642025374428928E-5	0.20651089287128857	2.4963038061643394E-5
46-47	0.11325730368567608	7.696936735673379E-5	4.160506343607232E-6	1.6642025374428928E-5	0.20789218097736617	2.9123544405250625E-5
48-49	0.11416845457492605	8.112987370034104E-5	4.160506343607232E-6	1.6642025374428928E-5	0.20935667921031592	2.9123544405250625E-5
50-51	0.11506088318562982	8.529038004394826E-5	6.2407595154108485E-6	1.6642025374428928E-5	0.21091686908916862	2.9123544405250625E-5
52-53	0.11590338572021028	9.569164590296634E-5	8.321012687214464E-6	1.6642025374428928E-5	0.21228567567621542	3.3284050748857856E-5
54-55	0.116319436354571	1.0401265859018081E-4	8.321012687214464E-6	1.6642025374428928E-5	0.21347141998414348	3.3284050748857856E-5
56-57	0.11710161154716917	1.1025341810559164E-4	8.321012687214464E-6	1.6642025374428928E-5	0.21461347897546368	3.3284050748857856E-5
58-59	0.11724098850968001	1.1441392444919889E-4	1.2481519030821697E-5	1.6642025374428928E-5	0.21567440809308353	3.3284050748857856E-5
60-61	0.11755094623227874	1.2273493713641336E-4	1.2481519030821697E-5	1.6642025374428928E-5	0.21648570683008692	3.3284050748857856E-5
62-63	0.11784218167633125	1.289756966518242E-4	1.2481519030821697E-5	1.6642025374428928E-5	0.21738645645347787	3.5364303920661474E-5
64-65	0.1178733854739083	1.3729670933903865E-4	1.2481519030821697E-5	1.6642025374428928E-5	0.2183724964569128	3.744455709246509E-5
66-67	0.11796075610712406	1.5393873471346758E-4	1.2481519030821697E-5	1.6642025374428928E-5	0.21916923342171357	3.744455709246509E-5
68-69	0.11804188598082438	1.5809924105707483E-4	1.2481519030821697E-5	1.6642025374428928E-5	0.2199846926650606	3.744455709246509E-5
70-71	0.11812925661404014	1.6225974740068205E-4	1.2481519030821697E-5	1.6642025374428928E-5	0.22080015190840763	4.576556977967955E-5
72-73	0.11817502218381983	1.6225974740068205E-4	1.4561772202625313E-5	1.6642025374428928E-5	0.2214803946955874	4.992607612328679E-5
74-75	0.11819790496870966	1.6225974740068205E-4	1.6642025374428928E-5	1.6642025374428928E-5	0.22225216862232655	4.992607612328679E-5
76-77	0.1182166272472559	1.7058076008789652E-4	2.4963038061643394E-5	1.6642025374428928E-5	0.22304266482761193	4.992607612328679E-5
78-79	0.11827071382972279	1.78901772775111E-4	2.4963038061643394E-5	1.6642025374428928E-5	0.22404950736276485	5.20063292950904E-5
80-81	0.11831855965267427	1.8722278546232546E-4	2.9123544405250625E-5	1.8722278546232545E-5	0.22489617040368892	5.4086582466894016E-5
82-83	0.11834768319707953	1.9762405132134353E-4	3.120379757705424E-5	2.0802531718036163E-5	0.22569290736848974	5.4086582466894016E-5
84-85	0.11836432522245396	2.0386481083675437E-4	3.3284050748857856E-5	2.0802531718036163E-5	0.22650004559914955	5.4086582466894016E-5
86-87	0.11837888699465658	2.0386481083675437E-4	3.3284050748857856E-5	2.0802531718036163E-5	0.22724477623465522	5.4086582466894016E-5
88-89	0.11841009079223364	2.0386481083675437E-4	3.3284050748857856E-5	2.0802531718036163E-5	0.228137204845359	5.824708881050125E-5
90-91	0.11842049205809264	2.1218582352396884E-4	3.3284050748857856E-5	2.0802531718036163E-5	0.22898594813945483	6.032734198230487E-5
92-93	0.11843713408346708	2.1218582352396884E-4	3.5364303920661474E-5	2.0802531718036163E-5	0.2296370673822294	6.240759515410848E-5
94-95	0.1184537761088415	2.1218582352396884E-4	3.744455709246509E-5	2.0802531718036163E-5	0.23035891523284524	6.656810149771571E-5
96-97	0.11845793661518511	2.1218582352396884E-4	3.744455709246509E-5	2.0802531718036163E-5	0.23107660257711748	6.656810149771571E-5
98-99	0.11846001686835692	2.1634632986757606E-4	3.744455709246509E-5	2.0802531718036163E-5	0.2317630861238127	6.656810149771571E-5
100-101	0.11847249838738774	2.1634632986757606E-4	3.744455709246509E-5	2.0802531718036163E-5	0.23239340283486917	6.656810149771571E-5
102-103	0.11849122066593398	2.225870893829869E-4	3.952481026426871E-5	2.0802531718036163E-5	0.23306116410301814	6.656810149771571E-5
104-105	0.11852034421033922	2.309081020702014E-4	4.1605063436072326E-5	2.0802531718036163E-5	0.23386830233367795	6.656810149771571E-5
106-107	0.11852866522302644	2.32988355242005E-4	4.1605063436072326E-5	2.0802531718036163E-5	0.2344798967661882	7.280886101312657E-5
108-109	0.11854530724840087	2.3922911475741585E-4	4.576556977967955E-5	2.0802531718036163E-5	0.23503948486940335	8.112987370034104E-5
110-111	0.11857235053963432	2.4546987427282666E-4	4.992607612328679E-5	2.0802531718036163E-5	0.23564275828922643	8.321012687214465E-5
112-113	0.11858483205866513	2.5379088696004116E-4	5.408658246689402E-5	2.0802531718036163E-5	0.23626891449393933	8.737063321575187E-5
114-115	0.11859731357769596	2.5379088696004116E-4	5.824708881050125E-5	2.0802531718036163E-5	0.23687842867327774	8.737063321575187E-5
116-117	0.11860355433721137	2.579513933036484E-4	5.824708881050125E-5	2.0802531718036163E-5	0.2374775415867572	8.737063321575187E-5
118-119	0.11861187534989859	2.6211189964725566E-4	5.824708881050125E-5	2.0802531718036163E-5	0.23814738310807798	8.737063321575187E-5
120-121	0.1186160358562422	2.6627240599086285E-4	5.824708881050125E-5	2.4963038061643394E-5	0.23880474311036792	8.737063321575187E-5
122-123	0.1186264371221012	2.683526591626665E-4	5.824708881050125E-5	2.4963038061643394E-5	0.23942465855556538	9.15311395593591E-5
124-125	0.11864099889430384	2.704329123344701E-4	5.824708881050125E-5	2.4963038061643394E-5	0.24018395096327372	9.15311395593591E-5
126-127	0.11865348041333465	2.704329123344701E-4	5.824708881050125E-5	2.9123544405250625E-5	0.2408766752694843	9.15311395593591E-5
128-129	0.11865556066650645	2.704329123344701E-4	5.824708881050125E-5	2.9123544405250625E-5	0.24165260970256708	9.15311395593591E-5
130-131	0.11866388167919367	2.7459341867807735E-4	5.824708881050125E-5	2.9123544405250625E-5	0.24250967400935017	9.361139273116273E-5
132-133	0.1186763631982245	2.7459341867807735E-4	6.240759515410848E-5	2.9123544405250625E-5	0.24320447856873256	9.569164590296634E-5
134-135	0.11868468421091172	2.8083417819348817E-4	6.240759515410848E-5	2.9123544405250625E-5	0.2438160730012428	9.985215224657358E-5
136-137	0.11869300522359892	2.829144313652918E-4	6.240759515410848E-5	2.9123544405250625E-5	0.24447135275036097	9.985215224657358E-5
138-139	0.11869924598311433	2.8707493770889904E-4	6.240759515410848E-5	2.9123544405250625E-5	0.24515575604388434	1.040126585901808E-4
>>END_MODULE
