##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	SnRK1a1OE.lowPi.4d.S.3.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24080481
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.98458083125499	37.0	35.0	38.0	33.0	39.0
2	35.4799691916453	35.0	34.0	38.0	33.0	39.0
3	35.62108643095626	36.0	35.0	38.0	33.0	39.0
4	35.702368528269844	36.0	35.0	38.0	33.0	39.0
5	35.634354770571235	36.0	35.0	38.0	33.0	39.0
6	35.5657927264825	36.0	35.0	37.0	33.0	39.0
7	35.534625201215874	35.0	34.0	38.0	33.0	39.0
8	35.67896222670967	36.0	35.0	38.0	33.0	39.0
9	35.66771402946644	36.0	35.0	38.0	33.0	39.0
10-14	35.58166969339192	36.0	35.0	37.8	33.0	39.0
15-19	35.59287135501986	36.0	35.0	38.0	33.0	39.0
20-24	35.57796320596752	36.0	35.0	38.0	33.0	39.0
25-29	35.558254920240174	36.0	35.0	38.0	33.0	39.0
30-34	35.53320061173197	36.0	35.0	38.0	33.0	39.0
35-39	35.50999404040143	36.0	35.0	38.0	33.0	39.0
40-44	35.488995240585105	36.0	34.0	38.0	33.0	39.0
45-49	35.45172621759507	36.0	34.0	38.0	33.0	39.0
50-54	35.432218999279954	36.0	34.0	38.0	33.0	39.0
55-59	35.39543164440943	36.0	34.0	38.0	32.4	39.0
60-64	35.35634055648639	36.0	34.0	38.0	32.0	39.0
65-69	35.31556057372775	36.0	34.0	38.0	32.0	39.0
70-74	35.28017577389754	36.0	34.0	38.0	32.0	39.0
75-79	35.22837507274045	36.0	34.0	38.0	32.0	39.0
80-84	35.18482469681565	36.0	34.0	38.0	32.0	39.0
85-89	35.13849642787451	36.0	34.0	38.0	32.0	39.0
90-94	35.077936973102815	36.0	34.0	38.0	32.0	39.0
95-99	35.00913804836374	35.8	34.0	37.2	31.4	39.0
100-104	34.95208724443669	35.4	34.0	37.0	31.0	39.0
105-109	34.88454559524787	35.0	34.0	37.0	31.0	39.0
110-114	34.80732219593122	35.0	34.0	37.0	31.0	39.0
115-119	34.72581940535158	35.0	34.0	37.0	30.8	39.0
120-124	34.64233128898048	35.0	34.0	37.0	30.0	39.0
125-129	34.5742609128115	35.0	34.0	37.0	30.0	39.0
130-134	34.47300522776102	35.0	34.0	37.0	30.0	39.0
135-139	34.37081314114947	35.0	34.0	37.0	29.8	39.0
140-144	34.26674253724417	35.0	34.0	37.0	29.0	39.0
145-149	34.16711049085772	35.0	34.0	37.0	29.0	39.0
150	34.09548978693574	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	19.0
21	226.0
22	1808.0
23	9228.0
24	30630.0
25	74960.0
26	144639.0
27	242411.0
28	348796.0
29	455086.0
30	583603.0
31	767326.0
32	1041021.0
33	1489035.0
34	2317491.0
35	4112663.0
36	1.048982E7
37	1971719.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.81578363252522	18.96866910069929	15.938687593273174	31.276859673502315
2	24.72885194684043	25.488849287424888	32.07194508049445	17.710353685240232
3	20.46528472583251	29.032775549624613	29.668946396876372	20.8329933276665
4	23.59952859745617	33.73311355367029	21.97548296481287	20.691874884060663
5	25.673623379865212	34.844387036953286	22.038372074046194	17.443617509135304
6	19.303916728241433	39.270370056146305	22.09618238107453	19.32953083453773
7	20.623728404760687	20.88330378450497	37.376524995493234	21.116442815241108
8	21.686760396802722	23.571901390669954	28.349571935443148	26.391766277084177
9	23.874228259809264	23.119363770183828	28.21729350007585	24.78911446993106
10-14	23.23515139864524	28.905883998269218	25.451889444050696	22.407075159034846
15-19	23.31967289191607	27.850723579815533	26.849769321468287	21.979834206800106
20-24	23.175167857737854	27.94290077416478	26.742403970126745	22.13952739797062
25-29	23.324701224404123	27.61134736146963	26.69506499222391	22.36888642190234
30-34	23.17178298888631	27.334474755716048	26.89719777607432	22.596544479323317
35-39	23.46977307861727	27.11812963468736	26.86318622010238	22.54891106659299
40-44	23.61010313705943	26.814329830039522	26.93816456573272	22.63740246716833
45-49	23.541661178127306	26.652541754897186	27.059330144271872	22.746466922703636
50-54	23.673439277840334	26.644169829034308	26.870897860145714	22.81149303297964
55-59	23.766853328220478	26.58861091686665	26.88930673768518	22.755229017227688
60-64	23.538646044110035	26.55397960045664	26.951137793340184	22.956236562093142
65-69	23.675216454355706	26.488479196075858	26.916721472465603	22.91958287710283
70-74	23.850635905907662	26.438100023318473	26.72564079368382	22.98562327709005
75-79	23.728437152065194	26.522213572062785	26.925962151669648	22.823387124202377
80-84	23.977581677043744	26.53844995870307	26.609920291874566	22.87404807237862
85-89	23.80751613723995	26.656346274810705	26.766233614685685	22.76990397326366
90-94	23.787214217191092	26.453998987810916	26.77293696915772	22.985849825840273
95-99	23.79642574723824	26.651272565611794	26.82048514366371	22.731816543486254
100-104	24.044606916282113	26.413994803509116	26.760806812787504	22.780591467421267
105-109	23.82293775610213	26.605981832339644	26.739522354225397	22.831558057332824
110-114	23.916721597047832	26.669216449621587	26.6590463869887	22.755015566341886
115-119	24.006329641059327	26.693729470717216	26.796901829302342	22.503039058921114
120-124	23.918569765434253	26.788650332928572	26.970801181012966	22.321978720624216
125-129	23.961951590584924	26.84155852202454	26.805190477715122	22.391299409675412
130-134	24.11294276057027	26.84959158415482	26.772863880916663	22.264601774358244
135-139	23.960839486553446	26.87797141593642	26.969036042095674	22.192153055414465
140-144	23.964785819100303	27.01830544029923	26.83510809996043	22.181800640640038
145-149	24.078718361149015	27.65299081858041	26.278303992349656	21.98998682792092
150	23.88704361843935	27.579204086496446	26.26957908357395	22.264173211490252
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	12.0
3	32.0
4	49.5
5	52.5
6	47.5
7	43.5
8	33.0
9	26.5
10	22.5
11	15.5
12	19.0
13	27.0
14	30.0
15	40.5
16	71.5
17	129.5
18	230.5
19	411.0
20	650.0
21	979.5
22	1483.5
23	2315.0
24	3758.0
25	5913.0
26	9173.5
27	14171.5
28	20896.0
29	29170.0
30	40101.5
31	56272.0
32	81550.0
33	111481.5
34	147257.5
35	206938.5
36	293653.0
37	392262.0
38	509217.0
39	664243.5
40	850555.0
41	1042199.0
42	1234993.0
43	1415956.0
44	1552425.5
45	1631481.0
46	1651580.5
47	1606450.5
48	1535390.5
49	1462647.5
50	1335232.0
51	1162981.5
52	992862.5
53	825162.0
54	671883.5
55	557211.0
56	458758.0
57	379650.5
58	320420.0
59	261821.5
60	200074.5
61	137248.5
62	85079.5
63	51128.0
64	31004.0
65	15279.0
66	8146.5
67	4238.5
68	2071.5
69	1187.0
70	778.0
71	586.5
72	375.0
73	277.0
74	231.0
75	161.0
76	113.5
77	45.0
78	9.5
79	3.5
80	1.0
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.661096387568006E-5
2	8.30548193784003E-6
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	4.152740968920015E-6
9	0.0
10-14	0.11059995022524675
15-19	0.0
20-24	1.6610963875680059E-6
25-29	8.305481937840029E-7
30-34	0.0
35-39	8.305481937840029E-7
40-44	0.0
45-49	8.305481937840029E-7
50-54	8.305481937840029E-7
55-59	0.0
60-64	3.3221927751360118E-6
65-69	0.0
70-74	8.305481937840029E-7
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	6.6443855502720235E-6
100-104	0.0
105-109	0.0
110-114	0.0
115-119	8.305481937840029E-7
120-124	8.305481937840029E-7
125-129	0.0
130-134	0.0
135-139	0.0
140-144	1.6610963875680059E-6
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4080481E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.438032252102644
#Duplication Level	Percentage of total
1	22.590133278175752
2	9.553392471241326
3	5.98507401945663
4	4.269108984512634
5	3.32136967558944
6	2.6314010557876792
7	2.1213141557395296
8	1.8214527526019395
9	1.6135211772355242
>10	19.204264690293357
>50	6.523594637913013
>100	12.375155449549116
>500	3.35400033001496
>1k	3.904277181243867
>5k	0.4779781560671277
>10k+	0.25396198457832936
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.0696861495416144E-4	8.30548193784003E-6	0.0	0.0	0.0030107372024670105	0.0
2	0.001482528525904445	1.661096387568006E-5	0.0	0.0	0.011071207423140758	0.0
3	0.0022590910870924877	1.661096387568006E-5	0.0	0.0	0.015008005861676933	0.0
4	0.005390257777658179	2.0763704844600072E-5	0.0	0.0	0.020850912404947394	0.0
5	0.0055771311212595796	2.0763704844600072E-5	0.0	0.0	0.024879071144799807	0.0
6	0.006449206724732782	2.0763704844600072E-5	0.0	0.0	0.032354004888855835	0.0
7	0.008180899708772428	2.0763704844600072E-5	0.0	0.0	0.043628696619473675	0.0
8	0.014563662578002491	2.4916445813520086E-5	0.0	0.0	0.058790353897000644	0.0
9	0.015049533271366133	2.4916445813520086E-5	0.0	0.0	0.06902270764441956	0.0
10-11	0.015680749898641974	2.4916445813520086E-5	0.0	0.0	0.11058541563185553	0.0
12-13	0.016478076164674615	2.4916445813520086E-5	0.0	0.0	0.1310625813496001	0.0
14-15	0.01934969654468281	2.4916445813520086E-5	0.0	0.0	0.1411662001269825	0.0
16-17	0.022501626940093097	2.4916445813520086E-5	0.0	0.0	0.14806182650587418	0.0
18-19	0.028581239718592	2.6992816297980093E-5	0.0	0.0	0.15618458784108175	0.0
20-21	0.03408154513192656	3.322192775136012E-5	0.0	0.0	0.16228911706539417	2.0763704844600073E-6
22-23	0.036427843779366365	4.1527409689200144E-5	8.30548193784003E-6	0.0	0.1678620954456848	4.152740968920015E-6
24-25	0.041294856194940624	4.1527409689200144E-5	8.30548193784003E-6	0.0	0.17388979896207224	4.152740968920015E-6
26-27	0.04569468525151138	4.1527409689200144E-5	8.30548193784003E-6	0.0	0.17871736033844174	4.152740968920015E-6
28-29	0.0565810957015352	4.1527409689200144E-5	8.30548193784003E-6	0.0	0.1826084786263198	4.152740968920015E-6
30-31	0.07849926253549504	4.568015065812016E-5	8.30548193784003E-6	0.0	0.1873052286621683	4.152740968920015E-6
32-33	0.0961380298009828	4.568015065812016E-5	8.30548193784003E-6	0.0	0.19117558324520179	4.152740968920015E-6
34-35	0.10949532112751402	4.568015065812016E-5	8.30548193784003E-6	0.0	0.1944001866075682	4.152740968920015E-6
36-37	0.11184577251592276	4.775652114258016E-5	1.4534593391220052E-5	0.0	0.19671326332725664	6.229111453380022E-6
38-39	0.11404257248848143	4.983289162704017E-5	1.661096387568006E-5	0.0	0.19856330942891048	8.30548193784003E-6
40-41	0.11530500574303312	5.190926211150018E-5	2.0763704844600072E-5	0.0	0.20020364211163388	8.30548193784003E-6
42-43	0.11559777398134198	6.021474404934021E-5	2.0763704844600072E-5	0.0	0.20189380768598436	8.30548193784003E-6
44-45	0.11635564920816989	6.644385550272023E-5	2.0763704844600072E-5	0.0	0.20322891390749215	1.0381852422300036E-5
46-47	0.11762431157417495	7.474933744056026E-5	2.0763704844600072E-5	0.0	0.20463254035498707	1.2458222906760043E-5
48-49	0.11872894067190767	7.890207840948028E-5	2.0763704844600072E-5	0.0	0.20608392332362463	1.2458222906760043E-5
50-51	0.11956779434762951	7.890207840948028E-5	2.0763704844600072E-5	0.0	0.20751246621693314	1.661096387568006E-5
52-53	0.1203422805383331	8.720756034732031E-5	2.0763704844600072E-5	0.0	0.20883719058601863	1.661096387568006E-5
54-55	0.12066411796342441	9.136030131624032E-5	2.4916445813520086E-5	2.0763704844600073E-6	0.21004356183748987	1.661096387568006E-5
56-57	0.12136177844620297	9.551304228516034E-5	2.4916445813520086E-5	4.152740968920015E-6	0.21126446768235235	1.661096387568006E-5
58-59	0.12149051341623948	9.551304228516034E-5	2.9069186782440103E-5	4.152740968920015E-6	0.21226320188537762	1.661096387568006E-5
60-61	0.12171476142856116	9.551304228516034E-5	2.9069186782440103E-5	4.152740968920015E-6	0.2131311247478819	1.661096387568006E-5
62-63	0.12192447484749162	9.551304228516034E-5	2.9069186782440103E-5	4.152740968920015E-6	0.2140509568724977	1.661096387568006E-5
64-65	0.12194316218185178	1.0174215373854036E-4	2.9069186782440103E-5	4.152740968920015E-6	0.21500401092486482	1.661096387568006E-5
66-67	0.12200545329638557	1.1004763567638037E-4	2.9069186782440103E-5	4.152740968920015E-6	0.21592384304948062	1.8687334360140065E-5
68-69	0.12205943892898152	1.2250585858314042E-4	2.9069186782440103E-5	4.152740968920015E-6	0.2167710022071403	2.0763704844600072E-5
70-71	0.12220478486289373	1.2458222906760043E-4	2.9069186782440103E-5	4.152740968920015E-6	0.21753095380445264	2.0763704844600072E-5
72-73	0.12226084686597415	1.2873497003652046E-4	2.9069186782440103E-5	4.152740968920015E-6	0.21809780294671025	2.6992816297980093E-5
74-75	0.12227745782984983	1.3081134052098046E-4	3.1145557266900107E-5	4.152740968920015E-6	0.21887851824886723	2.9069186782440103E-5
76-77	0.12230237427566334	1.3496408148990047E-4	3.529829823582013E-5	4.152740968920015E-6	0.2197049137016823	2.9069186782440103E-5
78-79	0.1223625890197127	1.3704045197436047E-4	3.737466872028013E-5	4.152740968920015E-6	0.22064135679017374	2.9069186782440103E-5
80-81	0.12241242191133972	1.4119319294328048E-4	3.737466872028013E-5	4.152740968920015E-6	0.22140961386942395	3.322192775136012E-5
82-83	0.12243733835715324	1.5157504536558053E-4	3.737466872028013E-5	4.152740968920015E-6	0.22212596168656265	3.322192775136012E-5
84-85	0.12247263665538907	1.5365141585004053E-4	3.737466872028013E-5	4.152740968920015E-6	0.22309770307328994	3.322192775136012E-5
86-87	0.12250585858314042	1.5365141585004053E-4	3.737466872028013E-5	4.152740968920015E-6	0.22387841837544692	3.737466872028013E-5
88-89	0.12253077502895394	1.5780415681896056E-4	3.737466872028013E-5	4.152740968920015E-6	0.224704813828262	4.1527409689200144E-5
90-91	0.122553615104283	1.6610963875680058E-4	3.737466872028013E-5	4.152740968920015E-6	0.22551252194671692	4.1527409689200144E-5
92-93	0.12255776784525194	1.6610963875680058E-4	3.9451039204740134E-5	4.152740968920015E-6	0.22613958583302385	4.1527409689200144E-5
94-95	0.12258268429106545	1.6610963875680058E-4	4.1527409689200144E-5	4.152740968920015E-6	0.22683724631580243	4.568015065812016E-5
96-97	0.12258476066154991	1.6610963875680058E-4	4.568015065812016E-5	4.152740968920015E-6	0.22754321228051883	4.568015065812016E-5
98-99	0.12260760073687897	1.7233875021018061E-4	4.568015065812016E-5	4.152740968920015E-6	0.22828447654347103	4.568015065812016E-5
100-101	0.12262628807123911	1.7649149117910062E-4	4.568015065812016E-5	4.152740968920015E-6	0.22894891509849824	4.775652114258016E-5
102-103	0.12264497540559925	1.7856786166356063E-4	4.983289162704017E-5	6.229111453380022E-6	0.22950953512930244	4.983289162704017E-5
104-105	0.12265950999899047	1.7856786166356063E-4	4.983289162704017E-5	8.30548193784003E-6	0.2301905846482053	4.983289162704017E-5
106-107	0.12266781548092831	1.8272060263248063E-4	4.983289162704017E-5	8.30548193784003E-6	0.23071798275125815	4.983289162704017E-5
108-109	0.12269273192674182	1.9310245505478068E-4	4.983289162704017E-5	8.30548193784003E-6	0.23117478425783938	4.983289162704017E-5
110-111	0.12272387748400873	1.9517882553924068E-4	4.983289162704017E-5	8.30548193784003E-6	0.2318122299965686	4.983289162704017E-5
112-113	0.1227301065954621	1.9517882553924068E-4	4.983289162704017E-5	8.30548193784003E-6	0.23252442507273838	4.983289162704017E-5
114-115	0.12273425933643103	1.9725519602370068E-4	4.983289162704017E-5	8.30548193784003E-6	0.2331099615493561	4.983289162704017E-5
116-117	0.1227363357069155	2.0556067796154072E-4	5.3985632595960186E-5	8.30548193784003E-6	0.233660199727738	4.983289162704017E-5
118-119	0.12274256481836887	2.0763704844600073E-4	5.3985632595960186E-5	8.30548193784003E-6	0.23427065265016925	4.983289162704017E-5
120-121	0.12275087030030671	2.0763704844600073E-4	5.3985632595960186E-5	8.30548193784003E-6	0.23497246587391674	5.3985632595960186E-5
122-123	0.12276125215272901	2.0763704844600073E-4	5.3985632595960186E-5	1.2458222906760043E-5	0.2355393150161743	5.3985632595960186E-5
124-125	0.12277371037563578	2.1178978941492074E-4	5.8138373564880206E-5	1.2458222906760043E-5	0.23623074638749947	5.3985632595960186E-5
126-127	0.12278824496902699	2.1178978941492074E-4	5.8138373564880206E-5	1.2458222906760043E-5	0.2368951849425267	5.81383735648802E-5
128-129	0.1227923977099959	2.2009527135276078E-4	5.8138373564880206E-5	1.2458222906760043E-5	0.23765721291032352	6.436748501826022E-5
130-131	0.12280070319193374	2.3255349425952082E-4	5.8138373564880206E-5	1.2458222906760043E-5	0.23845453917635615	6.852022598718024E-5
132-133	0.12281108504435606	2.3670623522844083E-4	5.8138373564880206E-5	1.2458222906760043E-5	0.23908367943314754	7.474933744056026E-5
134-135	0.12281523778532497	2.4085897619736084E-4	6.229111453380021E-5	1.2458222906760043E-5	0.23970659057848553	7.474933744056026E-5
136-137	0.12282769600823173	2.4293534668182084E-4	6.229111453380021E-5	1.2458222906760043E-5	0.24032742535333906	7.474933744056026E-5
138-139	0.12284015423113848	2.5539356958858086E-4	6.229111453380021E-5	1.4534593391220052E-5	0.2410105512427264	8.928393083178031E-5
>>END_MODULE
