##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.2d.R.1.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24169843
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.631724045538895	37.0	35.0	38.0	32.0	39.0
2	35.23725118942642	35.0	34.0	37.0	33.0	39.0
3	35.37588403035965	35.0	34.0	37.0	33.0	39.0
4	35.446289990381814	36.0	35.0	37.0	33.0	39.0
5	35.36771591772441	36.0	34.0	37.0	33.0	39.0
6	35.321375442943506	35.0	34.0	37.0	33.0	39.0
7	35.35370924833893	35.0	34.0	37.0	33.0	39.0
8	35.496651550446565	36.0	34.0	38.0	33.0	39.0
9	35.47353447848213	36.0	34.0	38.0	33.0	39.0
10-14	35.43592030779844	35.6	34.4	37.8	33.0	39.0
15-19	35.40839773762701	36.0	34.0	38.0	33.0	39.0
20-24	35.377689288258935	36.0	34.0	38.0	33.0	39.0
25-29	35.36856269194633	36.0	34.0	38.0	33.0	39.0
30-34	35.35159304923909	36.0	34.0	38.0	33.0	39.0
35-39	35.32813018272399	36.0	34.0	38.0	32.4	39.0
40-44	35.30952389719702	36.0	34.0	38.0	32.0	39.0
45-49	35.28359661252247	36.0	34.0	38.0	32.0	39.0
50-54	35.26099648226925	36.0	34.0	38.0	32.0	39.0
55-59	35.23286530243494	36.0	34.0	38.0	32.0	39.0
60-64	35.19388010919227	35.2	34.0	38.0	32.0	39.0
65-69	35.1609074332837	35.2	34.0	37.8	32.0	39.0
70-74	35.14215317823952	35.6	34.0	37.6	32.0	39.0
75-79	35.09419047529601	35.0	34.0	37.0	32.0	39.0
80-84	35.02238890008512	35.0	34.0	37.0	32.0	39.0
85-89	35.0082088824491	35.0	34.0	37.0	32.0	39.0
90-94	34.96010518562325	35.0	34.0	37.0	31.0	39.0
95-99	34.89861833194365	35.0	34.0	37.0	31.0	39.0
100-104	34.83778153627229	35.0	34.0	37.0	31.0	39.0
105-109	34.78283598284027	35.0	34.0	37.0	31.0	39.0
110-114	34.72167069517166	35.0	34.0	37.0	31.0	39.0
115-119	34.66585538846901	35.0	34.0	37.0	30.4	39.0
120-124	34.596153826899084	35.0	34.0	37.0	30.0	39.0
125-129	34.51893092561669	35.0	34.0	37.0	30.0	39.0
130-134	34.44770479477256	35.0	34.0	37.0	30.0	39.0
135-139	34.36041229560324	35.0	34.0	37.0	29.8	39.0
140-144	34.262436185456394	35.0	34.0	37.0	29.0	39.0
145-149	34.163853054403376	35.0	34.0	37.0	29.0	39.0
150	34.10036560849816	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	16.0
21	317.0
22	2347.0
23	10688.0
24	34384.0
25	81434.0
26	159846.0
27	275470.0
28	401843.0
29	524243.0
30	665025.0
31	855395.0
32	1132616.0
33	1565064.0
34	2335638.0
35	3987913.0
36	1.0485791E7
37	1651796.0
38	16.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.315761849001746	18.64161544787922	15.746457920577223	32.296164782541815
2	24.552213645095968	25.154240774691072	33.579939561869686	16.713606018343274
3	19.263244697121117	29.272738759618754	30.64285523079318	20.821161312466945
4	23.087754438454567	34.030084514822875	22.589604740088713	20.29255630663385
5	25.062438345172538	35.21240497921315	22.53444509341662	17.1907115821977
6	18.527244881152104	38.9914406974013	23.008246267880182	19.47306815356641
7	20.013526773839615	20.30004084014944	38.56499605727683	21.121436328734116
8	21.18756418897715	22.967641949515354	28.724634247727632	27.120159613779865
9	23.342001021686404	22.879846592300993	28.974913076597147	24.803239309415456
10-14	23.02853353246854	28.997419635700574	25.76575197447497	22.20829485735592
15-19	22.892837988231864	28.127856684877926	27.224872747415034	21.754432579475175
20-24	22.986051619901744	28.261319651191876	27.05179250781708	21.7008362210893
25-29	23.08472587099552	28.24581194011066	26.981134300293135	21.688327888600682
30-34	23.086753190742694	28.020644569350328	27.15153259373675	21.74106964617023
35-39	23.17691927084508	28.012674306572865	27.129681396772003	21.680725025810055
40-44	23.342413105455424	27.771168393605205	27.21278743928953	21.673631061649843
45-49	23.3522816014982	27.60777552423489	27.28004646120374	21.75989641306317
50-54	23.429509438100276	27.579302253467695	27.240655450187703	21.750532858244323
55-59	23.606896607536065	27.46428536970046	27.13089056582528	21.797927456938197
60-64	23.526453192103897	27.433267150307927	27.20853420520771	21.83174545238047
65-69	23.603739585730864	27.361161592981798	27.171304339875107	21.86379448141223
70-74	23.659592658504238	27.331277244953554	27.07837283014209	21.930757266400118
75-79	23.582360878388826	27.370032151222496	27.17618976672707	21.871417203661604
80-84	23.695148261812914	27.398208448120116	27.05840587165843	21.848237418408537
85-89	23.731992559455	27.321617123990844	27.111696019995563	21.83469429655859
90-94	23.659732502192917	27.259630937610975	27.16512556577219	21.91551099442392
95-99	23.741754549253795	27.40272578518611	27.14014650405466	21.715373161505436
100-104	23.88934776191428	27.36504434360657	26.95478681392169	21.790821080557464
105-109	23.665158271818314	27.50555806258237	27.058615978597793	21.770667687001524
110-114	23.701100582242095	27.508406239957782	27.00147783334795	21.789015344452174
115-119	23.784671667085302	27.499385080821586	27.01712956927358	21.698813682819537
120-124	23.74864101308925	27.40254479437492	27.201728428287502	21.64708576424833
125-129	23.732486801838142	27.488373838423364	27.164036605450853	21.615102754287648
130-134	23.780540072188305	27.39600253092252	27.23383432817499	21.589623068714182
135-139	23.705386284101113	27.419482430394627	27.320943932659752	21.55418735284451
140-144	23.720082914895226	27.54630305211333	27.299993632561037	21.433620400430403
145-149	23.813327593227708	27.93899071179779	26.943521640058876	21.304160054915627
150	23.455022028897744	27.907227200441476	27.09310523862319	21.544645532037592
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	4.0
2	13.5
3	29.5
4	38.5
5	26.5
6	14.5
7	17.0
8	12.5
9	7.5
10	7.5
11	8.5
12	9.0
13	12.0
14	22.5
15	35.0
16	72.0
17	144.5
18	248.5
19	417.5
20	635.0
21	943.5
22	1460.0
23	2499.0
24	4127.0
25	6767.0
26	10617.0
27	16711.0
28	27937.5
29	43598.0
30	58729.0
31	76518.5
32	101200.5
33	132688.5
34	179838.0
35	249625.0
36	347790.5
37	470459.0
38	616313.5
39	799773.5
40	1009047.5
41	1206949.0
42	1395061.0
43	1567411.0
44	1687748.0
45	1744625.0
46	1746051.0
47	1693166.5
48	1591121.5
49	1446664.5
50	1260030.0
51	1058397.0
52	865496.5
53	689975.0
54	540193.5
55	412337.5
56	309138.0
57	230508.5
58	167672.5
59	120341.5
60	83473.5
61	55953.0
62	38230.0
63	26891.0
64	18927.0
65	13904.5
66	10467.0
67	7740.5
68	6761.5
69	5148.5
70	3115.0
71	2374.5
72	1817.5
73	1402.0
74	1236.0
75	763.0
76	226.0
77	81.0
78	21.0
79	2.5
80	0.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.137387239131011E-6
2	8.274774478262022E-6
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.03024264576315204
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.274774478262023E-7
55-59	8.274774478262023E-7
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.05959989065713005
85-89	2.4824323434786065E-6
90-94	0.0
95-99	0.0
100-104	8.274774478262023E-7
105-109	0.0
110-114	0.0
115-119	0.0
120-124	8.274774478262023E-7
125-129	0.0
130-134	0.0
135-139	8.274774478262023E-7
140-144	0.0
145-149	0.03179995831996096
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4169843E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.11753254900623
#Duplication Level	Percentage of total
1	28.853947615615322
2	10.562235086433118
3	6.929879057935562
4	5.005025354932539
5	3.7664012259316317
6	3.083159770537258
7	2.6129297712184227
8	2.1262195908575547
9	1.8908562687673183
>10	21.66303780992412
>50	5.252976616562122
>100	6.277487570148484
>500	0.9663549221139431
>1k	0.8585661491518716
>5k	0.10148695883676323
>10k+	0.049436231034076454
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0012246666227827792	0.0
2	1.2412161717393035E-5	2.0686936195655058E-5	0.0	0.0	0.005014513333826786	0.0
3	4.964864686957214E-5	2.0686936195655058E-5	0.0	0.0	0.006632231744327011	0.0
4	1.2825900441306134E-4	2.0686936195655058E-5	0.0	0.0	0.008030668631153292	0.0
5	1.2825900441306134E-4	2.0686936195655058E-5	0.0	0.0	0.009528402811718718	0.0
6	1.3239639165219235E-4	2.0686936195655058E-5	0.0	0.0	0.011245418515958089	0.0
7	1.4067116613045437E-4	4.551125963044113E-5	0.0	0.0	0.013185853131110533	0.0
8	2.0273197471741956E-4	5.378603410870315E-5	0.0	4.137387239131011E-6	0.01909817949582875	0.0
9	2.151441364348126E-4	5.792342134783416E-5	0.0	8.274774478262022E-6	0.025552503588873125	0.0
10-11	2.4410584710872967E-4	6.206080858696516E-5	0.0	8.274774478262022E-6	0.03213508668633057	0.0
12-13	2.730675577826468E-4	6.206080858696516E-5	0.0	8.274774478262022E-6	0.038115679940494446	0.0
14-15	3.0823534931526033E-4	6.206080858696516E-5	0.0	8.274774478262022E-6	0.04618358505679991	2.0686936195655055E-6
16-17	3.5788399618483246E-4	6.412950220653067E-5	0.0	1.0343468097827527E-5	0.052315192945192074	4.137387239131011E-6
18-19	4.1787611115223213E-4	6.619819582609618E-5	0.0	1.2412161717393035E-5	0.06033551810824754	4.137387239131011E-6
20-21	4.695934516413698E-4	6.619819582609618E-5	0.0	1.2412161717393035E-5	0.06666365189049842	4.137387239131011E-6
22-23	5.5234119642399E-4	1.0343468097827529E-4	0.0	1.2412161717393035E-5	0.07403440725701033	4.137387239131011E-6
24-25	6.288828603479137E-4	1.1377814907610282E-4	0.0	1.2412161717393035E-5	0.07970469646823937	4.137387239131011E-6
26-27	6.806002008370514E-4	1.1998422993479933E-4	0.0	1.2412161717393035E-5	0.08388759496700082	4.137387239131011E-6
28-29	8.523017712609883E-4	1.1998422993479933E-4	6.2060808586965164E-6	1.6549548956524044E-5	0.08835804187888188	8.274774478262022E-6
30-31	0.0011026136992284144	1.2412161717393033E-4	1.2412161717393035E-5	1.6549548956524044E-5	0.09387111037502395	8.274774478262022E-6
32-33	0.0013032769803262686	1.3239639165219235E-4	1.2412161717393035E-5	1.6549548956524044E-5	0.10011856510611178	8.274774478262022E-6
34-35	0.001443948146456723	1.3239639165219235E-4	1.2412161717393035E-5	1.6549548956524044E-5	0.10360845124231878	8.274774478262022E-6
36-37	0.0015101463422828192	1.3239639165219235E-4	1.2412161717393035E-5	1.6549548956524044E-5	0.1070445513444171	8.274774478262022E-6
38-39	0.0015515202146741292	1.3446508527175787E-4	1.2412161717393035E-5	1.6549548956524044E-5	0.11048272014013497	8.274774478262022E-6
40-41	0.0015887566998263082	1.3653377889132336E-4	1.2412161717393035E-5	1.6549548956524044E-5	0.11337889120752667	8.274774478262022E-6
42-43	0.0016011688615437014	1.489459406087164E-4	1.2412161717393035E-5	1.6549548956524044E-5	0.11623782578976619	8.274774478262022E-6
44-45	0.0016301305722176184	1.6135810232610945E-4	1.2412161717393035E-5	1.6549548956524044E-5	0.1185589000309187	8.274774478262022E-6
46-47	0.0016839166063263218	2.0273197471741956E-4	1.2412161717393035E-5	1.861824257608955E-5	0.12108063755316906	1.0343468097827527E-5
48-49	0.0017335652531958938	2.151441364348126E-4	1.2412161717393035E-5	2.0686936195655058E-5	0.12338723093898459	1.2412161717393035E-5
50-51	0.0017894199809241624	2.2341891091307462E-4	1.2412161717393035E-5	2.0686936195655058E-5	0.12569175563118057	1.2412161717393035E-5
52-53	0.0018556181767502586	2.2341891091307462E-4	1.2412161717393035E-5	2.0686936195655058E-5	0.1278121665912352	1.2412161717393035E-5
54-55	0.0019156102917176582	2.3169368539133664E-4	1.2412161717393035E-5	2.0686936195655058E-5	0.1297008838658985	1.2412161717393035E-5
56-57	0.0019693963258263615	2.503119279674262E-4	1.2412161717393035E-5	2.0686936195655058E-5	0.1317095853704966	1.4480855336958538E-5
58-59	0.0020190449726959336	2.7720494502177776E-4	1.2412161717393035E-5	2.0686936195655058E-5	0.13359416525792078	1.6549548956524044E-5
60-61	0.002116273572815512	2.999605748369983E-4	1.2412161717393035E-5	2.0686936195655058E-5	0.13503811340437752	1.6549548956524044E-5
62-63	0.0021741969941633465	3.061666556956948E-4	1.2412161717393035E-5	2.0686936195655058E-5	0.13701164711744301	1.6549548956524044E-5
64-65	0.0021990213175981325	3.289222855109154E-4	1.2412161717393035E-5	2.0686936195655058E-5	0.13856937341297582	1.6549548956524044E-5
66-67	0.002296249917717711	3.5167791532613596E-4	1.6549548956524044E-5	2.482432343478607E-5	0.14001952764029124	2.0686936195655058E-5
68-69	0.002323142934772063	3.702961579022255E-4	1.6549548956524044E-5	2.482432343478607E-5	0.14130418637804143	2.0686936195655058E-5
70-71	0.00235210464544598	3.8063962600005306E-4	1.6549548956524044E-5	2.482432343478607E-5	0.14257643295407424	2.0686936195655058E-5
72-73	0.002403821985935118	4.033952558152736E-4	1.6549548956524044E-5	2.482432343478607E-5	0.14357354327870478	2.0686936195655058E-5
74-75	0.002422440228511207	4.0960133667397017E-4	1.6549548956524044E-5	2.482432343478607E-5	0.144653401348118	2.0686936195655058E-5
76-77	0.0024700201817612136	4.344256601087562E-4	1.6549548956524044E-5	2.482432343478607E-5	0.14601873913703123	2.0686936195655058E-5
78-79	0.0025155314413916547	4.6752475802180427E-4	1.6549548956524044E-5	2.482432343478607E-5	0.1476447323220097	2.0686936195655058E-5
80-81	0.0025258749094894824	4.840743069783283E-4	1.6549548956524044E-5	2.482432343478607E-5	0.14916522213239036	2.0686936195655058E-5
82-83	0.002552767926543834	5.006238559348524E-4	2.0686936195655055E-5	2.482432343478607E-5	0.15098360382398845	2.0686936195655058E-5
84-85	0.0025879357180764477	5.026925495544179E-4	2.482432343478607E-5	2.482432343478607E-5	0.15271510038356476	2.0686936195655058E-5
86-87	0.0026272408968481924	5.047612431739834E-4	2.896171067391708E-5	2.482432343478607E-5	0.15459347419013025	2.0686936195655058E-5
88-89	0.002647927833043847	5.068299367935489E-4	2.896171067391708E-5	2.482432343478607E-5	0.1563994437200109	2.0686936195655058E-5
90-91	0.002670683462859068	5.213107921305074E-4	2.896171067391708E-5	2.482432343478607E-5	0.15740689751273934	2.482432343478607E-5
92-93	0.002683095624576461	5.213107921305074E-4	3.516779153261359E-5	2.482432343478607E-5	0.15842883216080467	2.482432343478607E-5
94-95	0.002695507786293854	5.295855666087694E-4	4.1373872391310116E-5	2.482432343478607E-5	0.1594073242428592	2.482432343478607E-5
96-97	0.0027058512543916813	5.544098900435555E-4	4.1373872391310116E-5	2.896171067391708E-5	0.16022238952896797	2.6893017054351575E-5
98-99	0.0027182634161090746	5.585472772826866E-4	4.964864686957214E-5	2.896171067391708E-5	0.16113468341519638	2.896171067391708E-5
100-101	0.002728606884206902	5.60615970902252E-4	4.964864686957214E-5	2.896171067391708E-5	0.16187734442462037	3.309909791304809E-5
102-103	0.0027306755778264673	5.64753358141383E-4	5.1717340489137644E-5	2.896171067391708E-5	0.16266551669367485	3.309909791304809E-5
104-105	0.002738950352304729	5.750968262392105E-4	5.378603410870315E-5	2.896171067391708E-5	0.16357781057990323	3.309909791304809E-5
106-107	0.0027720494502177775	5.85440294337038E-4	5.378603410870315E-5	3.309909791304809E-5	0.16438046370429465	3.72364851521791E-5
108-109	0.0027761868374569084	5.895776815761692E-4	5.378603410870315E-5	3.309909791304809E-5	0.1652948262841426	3.9305178771744604E-5
110-111	0.0027927363864134326	5.957837624348656E-4	5.792342134783416E-5	3.309909791304809E-5	0.16625676881724055	4.1373872391310116E-5
112-113	0.002805148548130826	6.102646177718242E-4	5.792342134783416E-5	3.309909791304809E-5	0.16698908635856674	4.1373872391310116E-5
114-115	0.002811354628989522	6.164706986305207E-4	6.619819582609618E-5	3.309909791304809E-5	0.16787655592136036	4.1373872391310116E-5
116-117	0.0028134233226090877	6.206080858696517E-4	6.619819582609618E-5	3.309909791304809E-5	0.1688033306629257	4.1373872391310116E-5
118-119	0.002815492016228653	6.247454731087827E-4	6.619819582609618E-5	3.309909791304809E-5	0.16966804459590407	4.344256601087562E-5
120-121	0.002819629403467784	6.247454731087827E-4	6.619819582609618E-5	3.309909791304809E-5	0.17056378893317592	4.551125963044113E-5
122-123	0.0028258354843264805	6.247454731087827E-4	6.619819582609618E-5	3.309909791304809E-5	0.17127128215106735	4.757995325000663E-5
124-125	0.0028527285013808324	6.247454731087827E-4	6.619819582609618E-5	3.309909791304809E-5	0.17223115599054573	4.964864686957214E-5
126-127	0.0028672093567177906	6.392263284457412E-4	6.619819582609618E-5	3.309909791304809E-5	0.17327584626842632	4.964864686957214E-5
128-129	0.0028837589056743148	6.495697965435687E-4	6.619819582609618E-5	3.309909791304809E-5	0.174277093980296	4.964864686957214E-5
130-131	0.0029023771482504044	6.681880391196584E-4	6.619819582609618E-5	3.309909791304809E-5	0.17531350948369834	4.964864686957214E-5
132-133	0.0029313388589243218	6.702567327392238E-4	6.619819582609618E-5	3.309909791304809E-5	0.17620511643373107	5.5854727728268655E-5
134-135	0.002966506650456935	6.743941199783549E-4	6.619819582609618E-5	3.309909791304809E-5	0.17707396775394857	5.792342134783416E-5
136-137	0.0029727127313156315	6.743941199783549E-4	6.619819582609618E-5	3.309909791304809E-5	0.178081421546677	6.206080858696516E-5
138-139	0.002974781424935197	6.764628135979204E-4	6.619819582609618E-5	3.309909791304809E-5	0.17891303638174233	6.619819582609618E-5
>>END_MODULE
