##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.2d.R.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24201870
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.58121442681909	36.0	35.0	38.0	32.0	39.0
2	35.19972150912306	35.0	34.0	37.0	32.0	39.0
3	35.340037071515546	35.0	34.0	37.0	33.0	39.0
4	35.400593053346704	36.0	35.0	37.0	33.0	39.0
5	35.31462630780184	35.0	34.0	37.0	33.0	39.0
6	35.27666614191383	35.0	34.0	37.0	33.0	39.0
7	35.31735328716335	35.0	34.0	37.0	33.0	39.0
8	35.45883528834755	36.0	34.0	38.0	33.0	39.0
9	35.429860130642794	36.0	34.0	38.0	33.0	39.0
10-14	35.39212630263695	35.4	34.2	37.6	33.0	39.0
15-19	35.36328097787485	35.8	34.0	38.0	33.0	39.0
20-24	35.33420476186344	35.6	34.0	37.8	32.8	39.0
25-29	35.321771606904754	35.8	34.0	37.8	32.2	39.0
30-34	35.30500962942121	36.0	34.0	37.8	32.0	39.0
35-39	35.27796385981744	35.4	34.0	37.4	32.0	39.0
40-44	35.25893618137772	35.6	34.0	37.6	32.0	39.0
45-49	35.23073829418966	35.2	34.0	37.0	32.0	39.0
50-54	35.20823398357234	35.2	34.0	37.2	32.0	39.0
55-59	35.178208031032305	35.0	34.0	37.0	32.0	39.0
60-64	35.13924277752091	35.0	34.0	37.0	32.0	39.0
65-69	35.103420553866286	35.0	34.0	37.0	32.0	39.0
70-74	35.08413140802757	35.0	34.0	37.0	32.0	39.0
75-79	35.032171571866144	35.0	34.0	37.0	32.0	39.0
80-84	34.962498492885054	35.0	34.0	37.0	31.6	39.0
85-89	34.94280281647657	35.0	34.0	37.0	31.2	39.0
90-94	34.89277317000712	35.0	34.0	37.0	31.0	39.0
95-99	34.8291130644037	35.0	34.0	37.0	31.0	39.0
100-104	34.76711946638834	35.0	34.0	37.0	31.0	39.0
105-109	34.709138417816476	35.0	34.0	37.0	30.8	39.0
110-114	34.64554005124397	35.0	34.0	37.0	30.0	39.0
115-119	34.585693237753944	35.0	34.0	37.0	30.0	39.0
120-124	34.512511595178395	35.0	34.0	37.0	30.0	39.0
125-129	34.432420965817926	35.0	34.0	37.0	30.0	39.0
130-134	34.357265484030776	35.0	34.0	37.0	30.0	39.0
135-139	34.268087036249675	35.0	34.0	37.0	29.0	39.0
140-144	34.166613612915036	35.0	34.0	37.0	29.0	39.0
145-149	34.06537913805834	35.0	34.0	37.0	28.8	39.0
150	33.99388191904179	35.0	34.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	21.0
21	296.0
22	2340.0
23	11113.0
24	36378.0
25	85708.0
26	168946.0
27	288942.0
28	417839.0
29	544736.0
30	691489.0
31	889413.0
32	1178220.0
33	1627478.0
34	2417819.0
35	4071196.0
36	1.0183804E7
37	1586120.0
38	12.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.42342554521613	18.806964089964946	16.07722047924396	31.692389885574958
2	24.350217565832725	25.493290394502573	33.396807767333684	16.75968427233102
3	19.62957407836667	29.434969281299338	30.436226622157708	20.49923001817628
4	23.382714781242722	34.05371709102301	22.458203537916845	20.105364589817423
5	25.219985067269597	35.19066501886011	22.473676620856157	17.115673293014137
6	18.76193451167203	39.06372937297821	22.84323484094411	19.331101274405654
7	20.196740995633807	20.463447659209805	38.36015564086577	20.97965570429062
8	21.446875799266753	23.156404856318954	28.445930004582294	26.950789339832003
9	23.465840592724472	23.082618123418484	28.748436742633388	24.703104541223656
10-14	23.111111662032727	29.140769700853696	25.65536216829526	22.09275646881832
15-19	22.94273128481394	28.24951047171148	27.14273566464079	21.665022578833785
20-24	23.057836807634455	28.325822179847375	27.001270088210838	21.615070924307332
25-29	23.16908734738266	28.296268015653336	26.91922235761121	21.615422279352796
30-34	23.167460200389474	28.070133423574294	27.08432860766544	21.67807776837079
35-39	23.258402759786744	28.050702693634832	27.062584006938305	21.62831053964012
40-44	23.427890489453915	27.804727485933938	27.154150485065827	21.613231539546323
45-49	23.43730794355973	27.62722302036991	27.227233267511973	21.708235768558378
50-54	23.52264699812574	27.58224738486941	27.194248437779795	21.700857179225054
55-59	23.681280826646866	27.469485622391986	27.095798795712895	21.753434755248254
60-64	23.6098797324339	27.430296915073093	27.1573502378122	21.802473114680808
65-69	23.680525513111178	27.360480822349675	27.11546587102567	21.843527793513477
70-74	23.75431072061787	27.31435959287444	27.019359247859775	21.911970438647923
75-79	23.666089438543388	27.35588365692403	27.12194884114327	21.85607806338932
80-84	23.779715467767943	27.409749897246016	26.99022277169498	21.82031186329106
85-89	23.8277052461459	27.310345252745723	27.041956898718627	21.819992602389753
90-94	23.75142416681025	27.245241793299442	27.10751607210517	21.895817967785135
95-99	23.833879778711314	27.385874727861935	27.084035241904864	21.696210251521887
100-104	23.983311206943927	27.351599690437144	26.88616706064449	21.77892204197444
105-109	23.757589806076968	27.497965239876095	26.97683774022421	21.767607213822735
110-114	23.79046660444007	27.508522275344838	26.910524682596844	21.79048643761825
115-119	23.879139917700574	27.49747354233371	26.92886045582428	21.694526084141433
120-124	23.842572692461967	27.403510781633905	27.12204079372413	21.63187573218
125-129	23.82833888455727	27.48686361838982	27.067460489623326	21.61733700742959
130-134	23.875200552684564	27.398090312855988	27.14094737307489	21.585761761384553
135-139	23.798335417883	27.402913907065855	27.231340388160085	21.567410286891054
140-144	23.826586953818033	27.521485736432766	27.21141465514855	21.440512654600656
145-149	23.905662673129815	27.877183243049057	26.88819309168658	21.328960992134547
150	23.53497890865458	27.869970378322005	27.051364212765378	21.543686500258037
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	7.0
3	20.5
4	33.0
5	32.0
6	25.5
7	18.0
8	10.0
9	10.5
10	10.0
11	5.0
12	5.5
13	14.5
14	29.5
15	45.0
16	85.5
17	156.0
18	249.0
19	400.5
20	627.5
21	970.5
22	1506.0
23	2482.5
24	4102.5
25	6610.5
26	10282.5
27	16673.0
28	27969.5
29	43474.5
30	58708.5
31	75976.5
32	99693.0
33	131219.5
34	177304.0
35	246339.0
36	343882.5
37	466683.5
38	613520.5
39	796785.0
40	1008400.0
41	1205766.5
42	1391852.0
43	1565688.5
44	1685903.0
45	1744349.5
46	1748379.0
47	1696143.5
48	1595004.5
49	1451183.0
50	1264450.0
51	1063769.0
52	871200.0
53	695576.5
54	546424.0
55	417848.0
56	313428.0
57	233582.5
58	170178.0
59	121859.5
60	85053.5
61	57724.5
62	39152.5
63	27462.0
64	19197.5
65	13824.5
66	10526.0
67	7917.5
68	7019.5
69	5526.0
70	3275.0
71	2387.0
72	1832.5
73	1525.5
74	1315.0
75	788.0
76	266.0
77	92.5
78	25.0
79	1.5
80	0.5
81	1.0
82	1.0
83	0.5
84	0.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	4.131912120840249E-6
5	0.0
6	0.0
7	0.0
8	0.0
9	4.131912120840249E-6
10-14	0.0
15-19	0.0
20-24	0.026383085273989162
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.263824241680499E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.05181500437776089
85-89	8.263824241680499E-7
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	8.263824241680499E-7
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.026617777882452884
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.420187E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.690548084839165
#Duplication Level	Percentage of total
1	28.29093335846243
2	10.681552057778458
3	6.989788654441561
4	5.18417688918023
5	3.890882303557419
6	3.167624950154983
7	2.5412783695939116
8	2.211365392304775
9	1.8809242530935724
>10	21.263389549312745
>50	5.312348120302513
>100	6.456154159736371
>500	1.0149787652729532
>1k	0.9764774578684566
>5k	0.08265592387359127
>10k+	0.05546979506596167
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	8.263824241680498E-6	8.263824241680498E-6	0.0	0.0	0.0011569353938352697	0.0
2	2.4791472725041495E-5	3.718720908756224E-5	0.0	0.0	0.005511970769200892	0.0
3	3.718720908756224E-5	4.131912120840249E-5	0.0	0.0	0.007255637684195478	0.0
4	1.3635309998772822E-4	4.131912120840249E-5	0.0	0.0	0.008767917520423009	0.0
5	1.4048501210856847E-4	4.545103332924274E-5	0.0	0.0	0.010490924874813392	0.0
6	1.4874883635024896E-4	4.958294545008299E-5	0.0	0.0	0.01211063442618277	0.0
7	1.8180413331697097E-4	6.197868181260374E-5	0.0	0.0	0.014341866971436504	0.0
8	2.933657605796577E-4	6.197868181260374E-5	0.0	0.0	0.020473624558763434	0.0
9	3.098934090630187E-4	6.197868181260374E-5	0.0	4.131912120840249E-6	0.027002045709691027	0.0
10-11	3.5121253027142117E-4	6.197868181260374E-5	0.0	4.131912120840249E-6	0.033559390245464504	0.0
12-13	3.966635636006639E-4	6.404463787302386E-5	0.0	4.131912120840249E-6	0.0398936115267126	0.0
14-15	4.441805529903268E-4	6.817654999386411E-5	0.0	4.131912120840249E-6	0.04864913331077309	0.0
16-17	4.854996741987293E-4	7.024250605428423E-5	0.0	4.131912120840249E-6	0.05461354845720599	0.0
18-19	5.743357847967946E-4	7.024250605428423E-5	0.0	4.131912120840249E-6	0.06258194098224641	0.0
20-21	6.776335878178009E-4	7.850633029596473E-5	0.0	6.197868181260374E-6	0.06872609430593586	0.0
22-23	7.499420499325051E-4	8.677015453764524E-5	0.0	8.263824241680498E-6	0.07707875465821443	0.0
24-25	8.201845559867894E-4	8.883611059806536E-5	0.0	8.263824241680498E-6	0.08286962949557203	0.0
26-27	8.821632377993933E-4	9.090206665848549E-5	0.0	8.263824241680498E-6	0.08748084342242976	0.0
28-29	0.001078429063539305	9.296802271890561E-5	0.0	8.263824241680498E-6	0.09207966161292494	2.0659560604201246E-6
30-31	0.0014317075498711464	1.012318469605861E-4	0.0	8.263824241680498E-6	0.0980709341881433	4.131912120840249E-6
32-33	0.0016321052877318984	1.1156162726268672E-4	0.0	1.0329780302100622E-5	0.10486173175874426	4.131912120840249E-6
34-35	0.001807711552867609	1.1156162726268672E-4	0.0	1.2395736362520748E-5	0.10835113154479385	4.131912120840249E-6
36-37	0.0018800200149823132	1.1982545150436723E-4	0.0	1.2395736362520748E-5	0.11177442073691	4.131912120840249E-6
38-39	0.001941998696794917	1.2808927574604773E-4	0.0	1.2395736362520748E-5	0.11547248208506203	4.131912120840249E-6
40-41	0.0019853837740637396	1.3635309998772822E-4	0.0	1.2395736362520748E-5	0.1183854801302544	4.131912120840249E-6
42-43	0.0019998454664866805	1.4048501210856847E-4	0.0	1.2395736362520748E-5	0.12122410375727165	4.131912120840249E-6
44-45	0.0020205050270908818	1.5081479241066907E-4	0.0	1.2395736362520748E-5	0.12354830432524429	4.131912120840249E-6
46-47	0.0020638901043597046	2.024636939211722E-4	0.0	1.2395736362520748E-5	0.12647163215073876	4.131912120840249E-6
48-49	0.0021114070937493673	2.127934742232728E-4	0.0	1.2395736362520748E-5	0.12883301992779897	4.131912120840249E-6
50-51	0.0021692538634411306	2.1485943028369295E-4	0.0	1.2395736362520748E-5	0.13102499930790473	4.131912120840249E-6
52-53	0.002239496369495415	2.1485943028369295E-4	0.0	1.2395736362520748E-5	0.13306203198347896	4.131912120840249E-6
54-55	0.0022973431391871786	2.189913424045332E-4	0.0	1.2395736362520748E-5	0.1351486476045033	6.197868181260374E-6
56-57	0.0023965090300873447	2.272551666462137E-4	0.0	1.2395736362520748E-5	0.13721873557704423	8.263824241680498E-6
58-59	0.002464685580081208	2.437828151295747E-4	0.0	1.2395736362520748E-5	0.13926609803292062	8.263824241680498E-6
60-61	0.002549389778558434	2.5617855149209545E-4	0.0	1.2395736362520748E-5	0.14067921197824795	8.263824241680498E-6
62-63	0.002594840811887676	2.6857428785461617E-4	0.0	1.2395736362520748E-5	0.1426439361917075	8.263824241680498E-6
64-65	0.002619632284612718	2.7890406815671683E-4	0.0	1.2395736362520748E-5	0.14438966906276252	8.263824241680498E-6
66-67	0.002687808834606582	2.9749767270049793E-4	0.0	1.2395736362520748E-5	0.14574906815051897	8.263824241680498E-6
68-69	0.0027064024391503633	3.140253211838589E-4	0.0	1.2395736362520748E-5	0.14697004818222725	1.2395736362520748E-5
70-71	0.0027353258239962448	3.305529696672199E-4	0.0	1.6527648483360997E-5	0.14826540263211066	1.6527648483360997E-5
72-73	0.0027869747255067478	3.5534444239226145E-4	0.0	1.6527648483360997E-5	0.14918062116687678	2.0659560604201244E-5
74-75	0.002805568330050529	3.6360826663394194E-4	0.0	1.859360454378112E-5	0.15019293963648264	2.0659560604201244E-5
76-77	0.002834491714896411	3.7393804693604255E-4	0.0	2.0659560604201244E-5	0.15153787703181615	2.0659560604201244E-5
78-79	0.0028778767921652333	3.883997393589834E-4	0.0	2.0659560604201244E-5	0.15336831410134838	2.0659560604201244E-5
80-81	0.0028882065724673343	3.945976075402438E-4	0.0	2.4791472725041495E-5	0.15491364923454262	2.0659560604201244E-5
82-83	0.0029109320891319555	4.111252560236048E-4	4.131912120840249E-6	2.4791472725041495E-5	0.15679780116164577	2.0659560604201244E-5
84-85	0.002935723561856997	4.131912120840249E-4	4.131912120840249E-6	2.8923384845881743E-5	0.1582666959206045	2.4791472725041495E-5
86-87	0.002950185254279938	4.214550363257054E-4	4.131912120840249E-6	2.8923384845881743E-5	0.16013018828710343	2.4791472725041495E-5
88-89	0.0029791086391258194	4.338507726882262E-4	4.131912120840249E-6	2.8923384845881743E-5	0.16187592115815844	2.4791472725041495E-5
90-91	0.0030039001118508614	4.875656302591494E-4	4.131912120840249E-6	2.8923384845881743E-5	0.16281799712171002	2.4791472725041495E-5
92-93	0.0030121639360925416	4.916975423799897E-4	8.263824241680498E-6	2.8923384845881743E-5	0.16376833690950326	2.8923384845881743E-5
94-95	0.003014229892152962	5.020273226820902E-4	8.263824241680498E-6	2.8923384845881743E-5	0.16478065537910913	2.8923384845881743E-5
96-97	0.003018361804273802	5.102911469237708E-4	8.263824241680498E-6	2.8923384845881743E-5	0.1657000058259961	2.8923384845881743E-5
98-99	0.0030224937163946426	5.14423059044611E-4	8.263824241680498E-6	2.8923384845881743E-5	0.16653052016228498	2.8923384845881743E-5
100-101	0.0030266256285154825	5.185549711654513E-4	8.263824241680498E-6	3.3055296966721993E-5	0.16724534095919036	2.8923384845881743E-5
102-103	0.0030286915845759025	5.226868832862915E-4	1.0329780302100622E-5	3.3055296966721993E-5	0.1681130425045668	2.8923384845881743E-5
104-105	0.003039021364878003	5.309507075279721E-4	1.2395736362520748E-5	3.3055296966721993E-5	0.16889810580752646	2.8923384845881743E-5
106-107	0.0030782745300259855	5.743357847967946E-4	1.2395736362520748E-5	3.3055296966721993E-5	0.1696521797695798	3.3055296966721993E-5
108-109	0.003086538354267666	5.867315211593154E-4	1.6527648483360997E-5	3.3055296966721993E-5	0.17044963880890196	3.718720908756224E-5
110-111	0.0030927362224489265	6.032591696426763E-4	1.859360454378112E-5	3.3055296966721993E-5	0.17140204455275565	3.718720908756224E-5
112-113	0.0031133957830531277	6.177208620656172E-4	2.0659560604201244E-5	3.3055296966721993E-5	0.17217471211935276	3.9253165147982365E-5
114-115	0.003119593651234388	6.30116598428138E-4	2.0659560604201244E-5	3.3055296966721993E-5	0.17299902858746039	4.131912120840249E-5
116-117	0.0031237255633552283	6.363144666093984E-4	2.2725516664621368E-5	3.3055296966721993E-5	0.17382747696768885	4.7516989389662866E-5
118-119	0.0031278574754760686	6.363144666093984E-4	2.8923384845881743E-5	3.3055296966721993E-5	0.17455675945701715	4.958294545008299E-5
120-121	0.003131989387596909	6.404463787302386E-4	2.8923384845881743E-5	3.3055296966721993E-5	0.1753831418811852	4.958294545008299E-5
122-123	0.003131989387596909	6.487102029719192E-4	2.8923384845881743E-5	3.3055296966721993E-5	0.1760896988538489	4.958294545008299E-5
124-125	0.003140253211838589	6.507761590323392E-4	2.8923384845881743E-5	3.3055296966721993E-5	0.17692641105831905	5.7846769691763485E-5
126-127	0.00315471490426153	6.528421150927594E-4	2.8923384845881743E-5	3.3055296966721993E-5	0.1779077401870186	5.9912725752183616E-5
128-129	0.0031609127724427904	6.569740272135996E-4	2.8923384845881743E-5	3.3055296966721993E-5	0.17886840975511398	6.817654999386411E-5
130-131	0.0031629787285032103	6.569740272135996E-4	2.8923384845881743E-5	3.718720908756224E-5	0.17975883681715504	7.024250605428423E-5
132-133	0.003191902113349092	6.569740272135996E-4	3.3055296966721993E-5	3.718720908756224E-5	0.18051704269132923	7.437441817512448E-5
134-135	0.0032228914542553944	6.590399832740197E-4	3.3055296966721993E-5	3.718720908756224E-5	0.18129590812610763	7.850633029596473E-5
136-137	0.0032290893224366547	6.673038075157003E-4	3.3055296966721993E-5	3.9253165147982365E-5	0.18215327989118196	8.263824241680498E-5
138-139	0.003245616970920016	6.755676317573807E-4	3.3055296966721993E-5	4.545103332924274E-5	0.18294247510626244	8.470419847722511E-5
>>END_MODULE
