##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.2d.R.3.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24032968
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.61698996145628	37.0	35.0	38.0	32.0	39.0
2	35.25671814650608	35.0	34.0	37.0	33.0	39.0
3	35.40882948789346	35.0	34.0	37.0	33.0	39.0
4	35.46696720937672	36.0	35.0	38.0	33.0	39.0
5	35.38201469747723	36.0	35.0	37.0	33.0	39.0
6	35.3395249392418	35.0	34.0	37.0	33.0	39.0
7	35.37081229417856	35.0	34.0	37.0	33.0	39.0
8	35.518447284580084	36.0	34.0	38.0	33.0	39.0
9	35.48948269726819	36.0	34.0	38.0	33.0	39.0
10-14	35.45426962662289	35.8	34.4	37.8	33.0	39.0
15-19	35.42527361580974	36.0	34.0	38.0	33.0	39.0
20-24	35.39548371220733	36.0	34.0	38.0	33.0	39.0
25-29	35.38427068184005	36.0	34.0	38.0	33.0	39.0
30-34	35.367684074642796	36.0	34.0	38.0	33.0	39.0
35-39	35.3422074876478	36.0	34.0	38.0	32.8	39.0
40-44	35.32272789611337	36.0	34.0	38.0	32.2	39.0
45-49	35.29414431043224	36.0	34.0	38.0	32.0	39.0
50-54	35.27156255523662	36.0	34.0	38.0	32.0	39.0
55-59	35.242483508487176	36.0	34.0	38.0	32.0	39.0
60-64	35.202522942651115	35.8	34.0	38.0	32.0	39.0
65-69	35.16807404728371	35.6	34.0	37.6	32.0	39.0
70-74	35.14902933337239	35.6	34.0	37.6	32.0	39.0
75-79	35.09843888611677	35.0	34.0	37.0	32.0	39.0
80-84	35.02581965739729	35.0	34.0	37.0	32.0	39.0
85-89	35.008390956955466	35.0	34.0	37.0	32.0	39.0
90-94	34.96043586460066	35.0	34.0	37.0	31.6	39.0
95-99	34.89635057143171	35.0	34.0	37.0	31.0	39.0
100-104	34.83320234937274	35.0	34.0	37.0	31.0	39.0
105-109	34.77706267490557	35.0	34.0	37.0	31.0	39.0
110-114	34.71453653165103	35.0	34.0	37.0	31.0	39.0
115-119	34.65711119825067	35.0	34.0	37.0	30.4	39.0
120-124	34.585242313808266	35.0	34.0	37.0	30.0	39.0
125-129	34.50696851924407	35.0	34.0	37.0	30.0	39.0
130-134	34.4329186640618	35.0	34.0	37.0	30.0	39.0
135-139	34.34685611032312	35.0	34.0	37.0	29.8	39.0
140-144	34.24736273938366	35.0	34.0	37.0	29.0	39.0
145-149	34.146432259219914	35.0	34.0	37.0	29.0	39.0
150	34.07874878375405	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	21.0
21	244.0
22	1942.0
23	9319.0
24	30412.0
25	73904.0
26	150640.0
27	265139.0
28	392490.0
29	514260.0
30	657434.0
31	851804.0
32	1135276.0
33	1576758.0
34	2366594.0
35	4059484.0
36	1.037089E7
37	1576347.0
38	9.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.54595240329273	18.39387274936738	16.469149769909787	31.591025077430107
2	23.79326598362716	25.303795186678567	33.50440112099346	17.398537708700815
3	19.8378951779905	29.194729506567814	30.26588309858358	20.701492216858107
4	23.34700399884026	34.03002492243155	22.345920820100122	20.277050258628066
5	25.0806558723833	35.110361733099296	22.587335030779386	17.221647363738015
6	18.82045114028363	38.91176487232039	22.959170086690918	19.308613900705065
7	20.174611808246073	20.31791495748673	38.42101400043474	21.086459233832457
8	21.529267629366462	22.99127598389013	28.540465746885697	26.938990639857714
9	23.430922056734733	22.85808810630464	28.916349408029834	24.79464042893079
10-14	23.108606477568646	28.851743155485416	25.814712523230586	22.22493784371535
15-19	22.90127960891056	28.076002930640943	27.24078524134014	21.781932219108352
20-24	22.99296806786061	28.247719015467066	27.03593884156877	21.723374075103557
25-29	23.11850141121564	28.223845080007138	26.965042577887534	21.692610930889693
30-34	23.106276537348833	28.012600258216963	27.11356522518205	21.76755797925215
35-39	23.18674913560406	28.007903975905098	27.082494346932094	21.722852541558744
40-44	23.351259819428048	27.79599673248847	27.152690420925126	21.70005302715836
45-49	23.354629357472618	27.62095218534806	27.24273173417449	21.78168672300483
50-54	23.42945759699308	27.61157529616463	27.204570215418837	21.754396891423454
55-59	23.61006430832846	27.485017247973698	27.09928378384226	21.805634659855578
60-64	23.50580752240006	27.468326841695124	27.17676068973254	21.849104946172275
65-69	23.598060797151646	27.391666314372827	27.11612897749458	21.894143910980947
70-74	23.67254431495935	27.36354244719171	27.032911623732865	21.931001614116074
75-79	23.579896582061775	27.410934013643256	27.13279608244808	21.87637332184689
80-84	23.683431335420487	27.44110704886516	27.02177626197153	21.853685353742822
85-89	23.70752312995651	27.361630301855605	27.07669670326775	21.854149864920135
90-94	23.634607261158923	27.293333058155778	27.148668445778316	21.923391234906983
95-99	23.718946407285195	27.41894217975907	27.131449598734537	21.730661814221197
100-104	23.866541161291437	27.39271903495232	26.94601266060854	21.794727143147696
105-109	23.646331156434776	27.530458160640002	27.051565998839592	21.77164468408563
110-114	23.6775050006308	27.506615079752113	27.008955364980302	21.80692455463678
115-119	23.760099876136813	27.516892628492663	27.009832493431524	21.713175001939003
120-124	23.71885236979469	27.417155467439557	27.211059408059796	21.652932754705954
125-129	23.700479274969283	27.494865386580635	27.168442116679053	21.63621322177103
130-134	23.75029334703895	27.405146130931477	27.236139955747458	21.608420566282117
135-139	23.667627932327193	27.418823404739882	27.333773567490905	21.57977509544202
140-144	23.69706230208437	27.530157739984507	27.306891100591486	21.465888857339635
145-149	23.769261084567272	27.940215502814848	26.935961357316806	21.354562055301077
150	23.408831568368917	27.91343957184148	27.083787570474026	21.593941289315577
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	10.5
3	29.0
4	37.0
5	32.5
6	24.5
7	19.0
8	15.5
9	12.0
10	12.0
11	12.5
12	14.0
13	17.5
14	21.0
15	37.5
16	81.5
17	132.5
18	205.0
19	382.0
20	595.0
21	881.0
22	1395.5
23	2439.0
24	4107.0
25	6669.5
26	10569.0
27	16838.0
28	28698.0
29	44682.0
30	59933.0
31	76786.0
32	100266.0
33	132472.5
34	180042.5
35	248121.5
36	343264.5
37	463956.0
38	606457.0
39	786464.0
40	993857.5
41	1194056.5
42	1381374.0
43	1552680.0
44	1674191.5
45	1733441.5
46	1736820.0
47	1681919.0
48	1580103.5
49	1438817.0
50	1256658.5
51	1060491.0
52	871130.0
53	694589.5
54	543759.0
55	414819.0
56	310759.0
57	229774.0
58	166068.5
59	120184.0
60	84155.0
61	56561.5
62	38348.5
63	27240.5
64	19479.0
65	14076.0
66	10713.0
67	7910.5
68	6852.5
69	5258.0
70	3040.5
71	2337.5
72	1865.5
73	1488.0
74	1273.5
75	759.0
76	258.5
77	94.0
78	27.0
79	3.0
80	0.5
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.2482852721311825E-5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.030059541543100293
25-29	8.321901814207883E-7
30-34	8.321901814207883E-7
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.321901814207883E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.058776760323568863
85-89	8.321901814207883E-7
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	8.321901814207883E-7
140-144	0.0
145-149	0.03143848067371454
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4032968E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.592252146798494
#Duplication Level	Percentage of total
1	29.36606652677754
2	10.35894479988999
3	6.968280303346271
4	5.088233974706625
5	3.9782167176483205
6	3.095843556862571
7	2.5462717170640468
8	2.168275633253269
9	1.847368142740618
>10	21.481663761263587
>50	5.348982757948853
>100	6.072745438650946
>500	0.8668979303221361
>1k	0.73830314031785
>5k	0.07390559920740708
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0013356652411803652	0.0
2	2.912665634972759E-5	1.6643803628415766E-5	0.0	0.0	0.005650571331847152	0.0
3	7.0736165420767E-5	1.6643803628415766E-5	0.0	0.0	0.00709442129661222	0.0
4	1.206675763060143E-4	2.0804754535519707E-5	0.0	4.1609509071039415E-6	0.008579880770448328	0.0
5	1.206675763060143E-4	2.0804754535519707E-5	4.1609509071039415E-6	8.321901814207883E-6	0.010156881164240721	0.0
6	1.2482852721311825E-4	2.0804754535519707E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.011975216710645144	0.0
7	1.4563328174863795E-4	4.160950907103941E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.01430534921862335	0.0
8	1.9140374172678132E-4	4.5770459978143354E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.02003913956861258	0.0
9	1.9556469263388526E-4	4.5770459978143354E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.026542705836416044	0.0
10-11	2.3717420170492467E-4	4.785093543169533E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.033225192993224975	0.0
12-13	2.683813335082042E-4	5.201188633879927E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.03945205602570602	0.0
14-15	3.3079559711476333E-4	5.825331269945518E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.047977844434361996	0.0
16-17	3.599222534644909E-4	5.825331269945518E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.054109005595979656	0.0
18-19	4.3065841888525796E-4	6.241426360655912E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.062100111813072775	0.0
20-21	4.826703052240572E-4	7.281664087431897E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.06824167535195819	0.0
22-23	5.138774370273368E-4	9.570187086339066E-5	4.1609509071039415E-6	1.6643803628415766E-5	0.07737912354395846	0.0
24-25	5.617283724590321E-4	1.0194329722404657E-4	4.1609509071039415E-6	1.6643803628415766E-5	0.08334800762019906	0.0
26-27	5.950159797158636E-4	1.0818472358470248E-4	4.1609509071039415E-6	2.0804754535519707E-5	0.08814558401608989	0.0
28-29	7.052811787541182E-4	1.1650662539891036E-4	4.1609509071039415E-6	2.0804754535519707E-5	0.0929972527737731	0.0
30-31	8.946044450273475E-4	1.289894781202222E-4	8.321901814207883E-6	2.0804754535519707E-5	0.09978376370325961	0.0
32-33	0.0010256743986011216	1.4355280629508598E-4	1.2482852721311825E-5	2.0804754535519707E-5	0.1078372841839593	0.0
34-35	0.001144261499453584	1.4563328174863795E-4	1.2482852721311825E-5	2.0804754535519707E-5	0.11185260180931461	0.0
36-37	0.0012170781403279029	1.6435756083060568E-4	1.2482852721311825E-5	2.0804754535519707E-5	0.11559329667480105	0.0
38-39	0.0012670095512131501	1.7267946264481356E-4	1.2482852721311825E-5	2.0804754535519707E-5	0.11996229512726019	0.0
40-41	0.0012982166830164297	1.8724279081967738E-4	1.2482852721311825E-5	2.288522998907168E-5	0.12278550031773022	0.0
42-43	0.0013106995357377415	2.0180611899454118E-4	1.4563328174863796E-5	2.496570544262365E-5	0.1257626606917631	0.0
44-45	0.001346067618448125	2.2469134898361285E-4	1.6643803628415766E-5	2.496570544262365E-5	0.12815520746334785	0.0
46-47	0.0013876771275191644	2.475765789726845E-4	1.6643803628415766E-5	2.496570544262365E-5	0.1308951936356758	0.0
48-49	0.0014376085384044117	2.642203826011003E-4	1.6643803628415766E-5	2.496570544262365E-5	0.13342713226264855	0.0
50-51	0.0015000228020109708	2.787837107759641E-4	1.6643803628415766E-5	2.496570544262365E-5	0.1356033095870639	0.0
52-53	0.0015624370656175301	2.82944661683068E-4	1.6643803628415766E-5	2.496570544262365E-5	0.13799169540774156	0.0
54-55	0.0016019660992350176	2.9334703895082787E-4	2.080475453551971E-5	2.496570544262365E-5	0.13999519326951212	4.1609509071039415E-6
56-57	0.0016643803628415767	2.9542751440437984E-4	2.496570544262365E-5	2.496570544262365E-5	0.14207774919851762	4.1609509071039415E-6
58-59	0.0017226336755410319	3.0166894076503575E-4	2.704618089617562E-5	2.496570544262365E-5	0.14392729187672534	4.1609509071039415E-6
60-61	0.0017954503164153508	3.183127443934515E-4	2.912665634972759E-5	2.496570544262365E-5	0.1454626827614467	4.1609509071039415E-6
62-63	0.0018162550709508705	3.2871512166121136E-4	2.912665634972759E-5	2.496570544262365E-5	0.1475764458222555	6.241426360655913E-6
64-65	0.00184746220275415	3.5160035165028304E-4	2.912665634972759E-5	2.704618089617562E-5	0.14932196472778558	8.321901814207883E-6
66-67	0.0019306812208962289	3.848879589071146E-4	2.912665634972759E-5	3.328760725683153E-5	0.1507075613798512	8.321901814207883E-6
68-69	0.0019556469263388525	3.9737081162842644E-4	2.912665634972759E-5	3.328760725683153E-5	0.15202658281740317	8.321901814207883E-6
70-71	0.001980612631781476	4.0985366434973826E-4	2.912665634972759E-5	3.328760725683153E-5	0.15336224805858353	8.321901814207883E-6
72-73	0.0019993369108634437	4.202560416174981E-4	2.912665634972759E-5	3.328760725683153E-5	0.15427973773359993	8.321901814207883E-6
74-75	0.002011819763584756	4.2441699252460205E-4	2.912665634972759E-5	3.328760725683153E-5	0.15536574592035407	8.321901814207883E-6
76-77	0.002049268321748691	4.368998452459139E-4	3.328760725683153E-5	3.328760725683153E-5	0.15680335445875848	8.321901814207883E-6
78-79	0.0021116825853552505	4.618655506885375E-4	3.744855816393547E-5	3.328760725683153E-5	0.1585613562170099	8.321901814207883E-6
80-81	0.002126245913530114	4.7226792795629737E-4	3.744855816393547E-5	3.328760725683153E-5	0.16014875898807007	1.0402377267759855E-5
82-83	0.00214288971715853	4.785093543169533E-4	3.952903361748744E-5	3.328760725683153E-5	0.16192964597631054	1.2482852721311825E-5
84-85	0.0021595335207869456	4.909922070382651E-4	4.160950907103941E-5	3.328760725683153E-5	0.16360650919187342	1.2482852721311825E-5
86-87	0.002205303980765089	4.930726824918171E-4	4.160950907103941E-5	3.328760725683153E-5	0.16552054660914123	1.4563328174863796E-5
88-89	0.0022219477843935047	4.99314108852473E-4	4.160950907103941E-5	3.328760725683153E-5	0.16731807740101012	1.6643803628415766E-5
90-91	0.0022365111125683687	5.180383879344407E-4	4.160950907103941E-5	3.744855816393547E-5	0.1681897966160484	1.6643803628415766E-5
92-93	0.0022510744407432323	5.242798142950966E-4	4.160950907103941E-5	3.744855816393547E-5	0.16915097627558945	1.6643803628415766E-5
94-95	0.00225939634255744	5.430040933770644E-4	4.577045997814336E-5	3.744855816393547E-5	0.1701787311496441	2.288522998907168E-5
96-97	0.0022614768180109924	5.492455197377203E-4	5.201188633879927E-5	3.952903361748744E-5	0.17109622082466053	2.496570544262365E-5
98-99	0.00228020109709296	5.554869460983762E-4	5.825331269945518E-5	4.160950907103941E-5	0.17199706669604853	2.496570544262365E-5
100-101	0.0022906034743607195	5.67969798819688E-4	5.825331269945518E-5	4.160950907103941E-5	0.17273563548205947	3.120713180327956E-5
102-103	0.0022968449007213757	5.762917006338959E-4	5.825331269945518E-5	4.160950907103941E-5	0.17358238899165512	3.328760725683153E-5
104-105	0.0022989253761749274	5.887745533552077E-4	5.825331269945518E-5	4.160950907103941E-5	0.17446034963305407	3.328760725683153E-5
106-107	0.0023280520325246554	6.074988324371754E-4	5.825331269945518E-5	4.3689984524591383E-5	0.17534247122536012	3.53680827103835E-5
108-109	0.002342615360699519	6.179012097049353E-4	5.825331269945518E-5	4.5770459978143354E-5	0.1762724437530978	3.744855816393547E-5
110-111	0.002369661541595695	6.345450133333511E-4	5.825331269945518E-5	4.5770459978143354E-5	0.17721906008446398	3.744855816393547E-5
112-113	0.0023800639188634545	6.428669151475589E-4	5.825331269945518E-5	4.785093543169533E-5	0.1779326631650323	3.744855816393547E-5
114-115	0.002390466296131214	6.532692924153188E-4	6.033378815300715E-5	4.99314108852473E-5	0.17876277287099954	3.744855816393547E-5
116-117	0.002400868673398974	6.574302433224227E-4	6.241426360655912E-5	5.201188633879927E-5	0.17966153826693398	4.160950907103941E-5
118-119	0.0024029491488525264	6.657521451366306E-4	6.657521451366306E-5	5.409236179235124E-5	0.18049788939926187	4.3689984524591383E-5
120-121	0.00241751247702739	6.678326205901826E-4	6.657521451366306E-5	5.409236179235124E-5	0.1813113553016007	4.785093543169533E-5
122-123	0.002425834378841598	6.719935714972865E-4	7.281664087431897E-5	5.617283724590321E-5	0.18204160218579746	4.99314108852473E-5
124-125	0.00242791485429515	6.823959487650464E-4	7.489711632787095E-5	6.241426360655912E-5	0.18295909186081388	4.99314108852473E-5
126-127	0.002436236756109358	6.990397523934621E-4	7.489711632787095E-5	6.657521451366306E-5	0.18402637576848602	6.241426360655912E-5
128-129	0.0024445586579235658	7.05281178754118E-4	7.489711632787095E-5	6.657521451366306E-5	0.1849937968543877	7.697759178142292E-5
130-131	0.0024508000842842215	7.198445069289818E-4	7.489711632787095E-5	6.657521451366306E-5	0.18607356361478117	8.321901814207883E-5
132-133	0.0024903291179017087	7.260859332896377E-4	7.489711632787095E-5	6.657521451366306E-5	0.18695776568254074	8.52994935956308E-5
134-135	0.0025090533969836766	7.385687860109497E-4	7.489711632787095E-5	6.657521451366306E-5	0.18785653107847522	8.737996904918277E-5
136-137	0.0025173752987978845	7.656149669071253E-4	7.697759178142292E-5	7.0736165420767E-5	0.1887927450325736	8.737996904918277E-5
138-139	0.0025277776760656446	7.760173441748851E-4	8.946044450273474E-5	7.0736165420767E-5	0.18959580855764466	9.154091995628672E-5
>>END_MODULE
