##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.2d.S.1.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23616406
Total Bases	3.5 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.777563698727064	37.0	35.0	38.0	33.0	39.0
2	35.340859316188926	35.0	34.0	37.0	33.0	39.0
3	35.43398453600434	35.0	34.0	37.0	33.0	39.0
4	35.54066351162832	36.0	35.0	38.0	33.0	39.0
5	35.46964334878051	36.0	35.0	38.0	33.0	39.0
6	35.40453483904367	35.0	35.0	37.0	33.0	39.0
7	35.45555098434537	35.0	34.0	38.0	33.0	39.0
8	35.561570503149376	36.0	35.0	38.0	33.0	39.0
9	35.57555764412248	36.0	35.0	38.0	33.0	39.0
10-14	35.534443225611895	35.8	35.0	37.8	33.0	39.0
15-19	35.50229014524903	36.0	35.0	38.0	33.0	39.0
20-24	35.47853785203388	36.0	34.8	38.0	33.0	39.0
25-29	35.46524315342479	36.0	34.6	38.0	33.0	39.0
30-34	35.454090406474215	36.0	34.0	38.0	33.0	39.0
35-39	35.42021016237611	36.0	34.0	38.0	33.0	39.0
40-44	35.41104917488292	36.0	34.0	38.0	33.0	39.0
45-49	35.37647933390034	36.0	34.0	38.0	32.6	39.0
50-54	35.35879212950523	36.0	34.0	38.0	32.0	39.0
55-59	35.32474208819073	36.0	34.0	38.0	32.0	39.0
60-64	35.2840255286939	36.0	34.0	38.0	32.0	39.0
65-69	35.244476581237635	36.0	34.0	38.0	32.0	39.0
70-74	35.22818784534785	36.0	34.0	38.0	32.0	39.0
75-79	35.16494718120954	36.0	34.0	38.0	32.0	39.0
80-84	35.093361149024965	36.0	34.0	38.0	32.0	39.0
85-89	35.07027844118195	36.0	34.0	38.0	32.0	39.0
90-94	35.0218193826783	35.6	34.0	38.0	31.6	39.0
95-99	34.95331009299213	35.2	34.0	37.6	31.0	39.0
100-104	34.89055894448969	35.0	34.0	37.4	31.0	39.0
105-109	34.83242117365361	35.0	34.0	37.2	31.0	39.0
110-114	34.75868697379271	35.0	34.0	37.0	30.8	39.0
115-119	34.695250191752294	35.0	34.0	37.0	30.0	39.0
120-124	34.620513527756934	35.0	34.0	37.0	30.0	39.0
125-129	34.5377759765817	35.0	34.0	37.0	30.0	39.0
130-134	34.455386767995094	35.0	34.0	37.0	30.0	39.0
135-139	34.37129386241073	35.0	34.0	37.0	29.6	39.0
140-144	34.27249602670279	35.0	34.0	37.0	29.0	39.0
145-149	34.167742814042064	35.0	34.0	37.0	29.0	39.0
150	34.08793471792448	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	9.0
21	204.0
22	1631.0
23	7929.0
24	26650.0
25	65420.0
26	135883.0
27	244353.0
28	366109.0
29	486797.0
30	630875.0
31	825625.0
32	1104747.0
33	1539391.0
34	2306049.0
35	3912176.0
36	9851583.0
37	2110967.0
38	6.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	31.67062895474565	19.147448563826714	12.548154556123169	36.63376792530447
2	25.202458833067148	25.569280101299068	32.92060189005897	16.30765917557481
3	19.030173346443995	29.805923898835413	30.237678840717763	20.926223914002833
4	22.709183607361762	34.31083459523858	21.64321277335764	21.336769024042017
5	25.79785425436876	35.24670519299168	21.32008147217659	17.635359080462962
6	18.907462041430012	39.01588158672408	21.865901187504992	20.21075518434092
7	20.244684987207624	20.473013548293505	37.107657278588455	22.174644185910424
8	20.740348891359677	23.118200119018955	28.889827690123553	27.251623299497812
9	23.2836656009386	23.079659114939	28.1078247045719	25.528850579550504
10-14	23.121815402394418	28.715429434944507	25.147192168020826	23.015562994640252
15-19	23.364002973187368	27.578342784249223	26.52942789008624	22.52822635247717
20-24	23.26449355607688	27.567051495122	26.424286311137422	22.744168637663694
25-29	23.35541656931203	27.194357176955712	26.43088961123043	23.019336642501827
30-34	23.151377055424945	26.908788746263934	26.641395816111903	23.298438382199222
35-39	23.50671986245494	26.695419277598802	26.66192391848277	23.135936941463488
40-44	23.715058929796516	26.36609905842574	26.626324090126165	23.292517921651584
45-49	23.639826483335355	26.21904704720947	26.70607881656506	23.435047652890116
50-54	23.78035949907331	26.173727926033518	26.506125669639363	23.539786905253806
55-59	23.83769825095317	26.234126395015394	26.50121614609776	23.42695920793367
60-64	23.50423768968064	26.18832603064158	26.610903454149625	23.696532825528152
65-69	23.69790983437531	26.135766805499532	26.616767174480316	23.549556185644843
70-74	23.89805544501564	26.116418391519865	26.31655807407782	23.668968089386674
75-79	23.711130304924467	26.18402647718709	26.66420368958765	23.440639528300792
80-84	24.062451040589576	26.229420440978714	26.30191351814826	23.406215000283456
85-89	23.75475377167472	26.317740748829284	26.567572162632576	23.359933316863426
90-94	23.733455463121697	26.0323581835441	26.636097804212884	23.598088549121318
95-99	23.742701577877686	26.254440239552114	26.67086092608672	23.33199725648348
100-104	24.030731213129815	26.015694564326974	26.565783350487653	23.38779087205556
105-109	23.829433657263515	26.191533123202575	26.52831679807673	23.450716421457184
110-114	23.890434471697343	26.350990070207974	26.409365591021768	23.349209867072915
115-119	24.003827678098013	26.379080711942365	26.56960419803081	23.04748741192881
120-124	23.96450586088332	26.579786102932	26.63900679891767	22.816701237267008
125-129	23.957487011359817	26.630035069688418	26.532519808475513	22.879958110476252
130-134	24.146607235664902	26.611005078418792	26.54084452985776	22.701543156058545
135-139	23.87973767049906	26.66442726297981	26.83250025427239	22.62333481224874
140-144	23.919771907035916	26.857600829335343	26.61420900889161	22.608418254737135
145-149	24.091738671178344	27.488564766652928	26.063624772953943	22.356071789214784
150	23.863931709168618	27.530907962879702	25.98288240810223	22.62227791984945
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	3.5
2	7.5
3	15.0
4	29.5
5	30.0
6	18.0
7	19.5
8	14.5
9	5.5
10	6.5
11	6.0
12	5.5
13	9.0
14	20.0
15	31.5
16	52.5
17	93.0
18	178.5
19	325.0
20	496.5
21	724.5
22	1117.0
23	1814.0
24	2886.0
25	4650.5
26	7203.0
27	10717.0
28	16219.5
29	23312.5
30	32668.0
31	46196.0
32	65352.0
33	89247.0
34	120368.5
35	169483.5
36	239320.5
37	325308.0
38	434240.5
39	566583.0
40	724236.0
41	903315.0
42	1094349.0
43	1280555.0
44	1429803.0
45	1538571.5
46	1584538.0
47	1562014.0
48	1532263.0
49	1501270.0
50	1399968.0
51	1245578.0
52	1072432.5
53	901148.0
54	760167.0
55	647287.0
56	541608.0
57	443237.5
58	370538.5
59	305640.0
60	231854.5
61	156109.5
62	94388.5
63	56689.0
64	35608.5
65	18398.5
66	10262.5
67	5705.0
68	3181.0
69	1931.5
70	1194.5
71	1071.5
72	890.0
73	708.5
74	581.5
75	361.0
76	122.5
77	36.0
78	10.0
79	2.0
80	1.0
81	0.0
82	0.5
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.234344548446533E-6
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.024775996821870358
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.468689096893066E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.04824358117827073
85-89	1.6937378193786132E-6
90-94	0.0
95-99	0.0
100-104	8.468689096893066E-7
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	8.468689096893066E-7
145-149	0.0263325418778793
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.3616406E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.19926567824506
#Duplication Level	Percentage of total
1	24.101615555205814
2	7.946972186220528
3	5.003693124968319
4	3.654824732314837
5	2.6708742913129035
6	2.1605410770755036
7	1.7560091798134077
8	1.4779115159014253
9	1.3107290391528204
>10	17.03985990868374
>50	6.141304216007488
>100	13.405059929060961
>500	4.9056420049591045
>1k	6.673435176905729
>5k	1.0592555540795696
>10k+	0.692272508337907
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	29807	0.12621310795554583	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.556849420695088E-4	4.234344548446533E-6	0.0	0.0	0.0022823117116126815	0.0
2	0.0013719276336966767	1.6937378193786134E-5	0.0	4.234344548446533E-6	0.010801812943087106	0.0
3	0.00221032785428909	2.1171722742232667E-5	0.0	4.234344548446533E-6	0.015866089023029162	0.0
4	0.005767177274984179	2.1171722742232667E-5	0.0	4.234344548446533E-6	0.02502497628131901	0.0
5	0.005978894502406505	2.1171722742232667E-5	0.0	4.234344548446533E-6	0.03130027490211677	0.0
6	0.007092527118647943	2.1171722742232667E-5	0.0	4.234344548446533E-6	0.040929174405284194	0.0
7	0.009095372090063154	2.1171722742232667E-5	0.0	4.234344548446533E-6	0.054783949767801245	0.0
8	0.0162429456878409	2.1171722742232667E-5	0.0	4.234344548446533E-6	0.0723226048874668	0.0
9	0.0169162064710439	2.1171722742232667E-5	0.0	4.234344548446533E-6	0.0835351492517532	0.0
10-11	0.0176529824224736	2.1171722742232667E-5	0.0	4.234344548446533E-6	0.14024360861682342	0.0
12-13	0.018269079554272566	2.1171722742232667E-5	0.0	4.234344548446533E-6	0.1663504599302705	0.0
14-15	0.020767342837856022	2.54060672906792E-5	0.0	4.234344548446533E-6	0.17788481448023885	0.0
16-17	0.023693274920832577	2.54060672906792E-5	0.0	4.234344548446533E-6	0.18607615400920868	0.0
18-19	0.02944986633444564	3.1757584113349E-5	0.0	4.234344548446533E-6	0.19358788123815285	0.0
20-21	0.03461576668355041	3.387475638757227E-5	0.0	4.234344548446533E-6	0.19767402372740372	0.0
22-23	0.03668424399546655	5.504647912980493E-5	0.0	4.234344548446533E-6	0.2023762633484536	0.0
24-25	0.04144576444019467	5.928082367825146E-5	0.0	4.234344548446533E-6	0.2072965717137485	0.0
26-27	0.045597539269946495	6.774951277514453E-5	0.0	4.234344548446533E-6	0.211274738417014	0.0
28-29	0.0563993522130336	7.198385732359107E-5	0.0	4.234344548446533E-6	0.21479983025359575	0.0
30-31	0.07995077659149322	7.198385732359107E-5	0.0	4.234344548446533E-6	0.21840114029204952	0.0
32-33	0.1011055619555321	7.198385732359107E-5	0.0	4.234344548446533E-6	0.22128049458499316	0.0
34-35	0.1167938085075265	7.198385732359107E-5	0.0	4.234344548446533E-6	0.22495590565304474	0.0
36-37	0.11856164735650293	7.198385732359107E-5	0.0	4.234344548446533E-6	0.2269079384898786	0.0
38-39	0.12037182965096382	7.621820187203761E-5	0.0	4.234344548446533E-6	0.228618613687451	0.0
40-41	0.12175010880148318	8.680406324315394E-5	0.0	4.234344548446533E-6	0.23038221819187898	0.0
42-43	0.12200628664666419	8.89212355173772E-5	0.0	4.234344548446533E-6	0.2318028407878828	0.0
44-45	0.12271977370307743	9.103840779160047E-5	0.0	4.234344548446533E-6	0.23307102698014254	0.0
46-47	0.12340785469219999	1.0374144143694007E-4	0.0	4.234344548446533E-6	0.23443660309701653	0.0
48-49	0.12418697408911415	1.0585861371116334E-4	0.0	4.234344548446533E-6	0.23552694681824152	0.0
50-51	0.12493857024646342	1.0585861371116334E-4	0.0	4.234344548446533E-6	0.23664693095130562	0.0
52-53	0.1257579159165878	1.0585861371116334E-4	0.0	4.234344548446533E-6	0.237826195908048	0.0
54-55	0.12617711602688403	1.0585861371116334E-4	0.0	4.234344548446533E-6	0.23874928301960935	4.234344548446533E-6
56-57	0.12690754046149105	1.143273028080564E-4	0.0	4.234344548446533E-6	0.23968507316481602	4.234344548446533E-6
58-59	0.1270409223147671	1.2279599190494946E-4	0.0	4.234344548446533E-6	0.24054464510815066	4.234344548446533E-6
60-61	0.12725263954218946	1.27030336453396E-4	0.0	4.234344548446533E-6	0.24124119478637013	4.234344548446533E-6
62-63	0.1275130517319189	1.3338185327606581E-4	0.0	4.234344548446533E-6	0.24219180513749639	4.234344548446533E-6
64-65	0.12754480931603224	1.460848869214054E-4	0.0	4.234344548446533E-6	0.2430873690094928	4.234344548446533E-6
66-67	0.12760620731198474	1.7572529876053113E-4	0.0	4.234344548446533E-6	0.2437775671708896	4.234344548446533E-6
68-69	0.1276570194465661	1.7995964330897766E-4	0.0	4.234344548446533E-6	0.244615967391482	4.234344548446533E-6
70-71	0.12775440937118038	1.8631116013164746E-4	0.0	4.234344548446533E-6	0.24551153126347847	4.234344548446533E-6
72-73	0.1278136901948586	1.884283324058707E-4	0.0	4.234344548446533E-6	0.24600271523109823	8.468689096893067E-6
74-75	0.12783486191760085	2.0113136605121035E-4	0.0	4.234344548446533E-6	0.24664210125791367	1.4820205919562867E-5
76-77	0.12786450232943997	2.0960005514810342E-4	0.0	4.234344548446533E-6	0.2473555883143269	1.6937378193786134E-5
78-79	0.12793225184221513	2.3288895016455933E-4	0.0	4.234344548446533E-6	0.24861530581748975	1.6937378193786134E-5
80-81	0.1280232902500067	2.3712329471300587E-4	0.0	4.234344548446533E-6	0.24955109596269642	1.6937378193786134E-5
82-83	0.12804657914502315	2.455919838098989E-4	2.1171722742232667E-6	4.234344548446533E-6	0.25051652651974227	1.6937378193786134E-5
84-85	0.1280846882459592	2.54060672906792E-4	4.234344548446533E-6	6.3515168226698E-6	0.2513655126017058	1.6937378193786134E-5
86-87	0.12813126603599212	2.5617784518101525E-4	4.234344548446533E-6	8.468689096893067E-6	0.25219120978865284	1.9054550468009402E-5
88-89	0.1281693751369281	2.5829501745523855E-4	4.234344548446533E-6	8.468689096893067E-6	0.25306348476563284	2.1171722742232667E-5
90-91	0.12820748423786416	2.6252936200368506E-4	4.234344548446533E-6	1.27030336453396E-5	0.2536986364478998	2.752323956490247E-5
92-93	0.1282392418219775	2.6252936200368506E-4	4.234344548446533E-6	1.27030336453396E-5	0.25424698406692364	2.9640411839125734E-5
94-95	0.12831969436839796	2.6676370655213163E-4	4.234344548446533E-6	1.27030336453396E-5	0.25511290752708093	2.9640411839125734E-5
96-97	0.12833451457431752	2.837010847459178E-4	4.234344548446533E-6	1.27030336453396E-5	0.25580522286075197	2.9640411839125734E-5
98-99	0.12835356912478554	3.0063846293970387E-4	4.234344548446533E-6	1.4820205919562867E-5	0.256393796752986	2.9640411839125734E-5
100-101	0.1283832095366247	3.048728074881504E-4	4.234344548446533E-6	1.6937378193786134E-5	0.2570056595402366	2.9640411839125734E-5
102-103	0.12840438125936687	3.048728074881504E-4	4.234344548446533E-6	1.6937378193786134E-5	0.25764081122250354	2.9640411839125734E-5
104-105	0.12842767015438333	3.0910715203659694E-4	4.234344548446533E-6	1.6937378193786134E-5	0.25832889221162614	2.9640411839125734E-5
106-107	0.12844672470485136	3.1334149658504345E-4	4.234344548446533E-6	1.6937378193786134E-5	0.25899791865028066	3.387475638757227E-5
108-109	0.12847213077214203	3.2392735795615983E-4	6.3515168226698E-6	2.1171722742232667E-5	0.25962883598799924	3.5991928661795536E-5
110-111	0.12850177118398115	3.366303916014994E-4	8.468689096893067E-6	2.1171722742232667E-5	0.2602279957416044	3.81091009360188E-5
112-113	0.12852929442354608	3.5145059752106226E-4	8.468689096893067E-6	2.1171722742232667E-5	0.26083350701203223	3.81091009360188E-5
114-115	0.12855470049083675	3.535677697952855E-4	1.0585861371116334E-5	2.1171722742232667E-5	0.2614877132447672	3.81091009360188E-5
116-117	0.12857163786903053	3.5991928661795533E-4	1.27030336453396E-5	2.1171722742232667E-5	0.2621270992715826	3.81091009360188E-5
118-119	0.1285970439363212	3.5991928661795533E-4	1.27030336453396E-5	2.1171722742232667E-5	0.2626987357856229	3.81091009360188E-5
120-121	0.1286055126254181	3.5991928661795533E-4	1.27030336453396E-5	2.54060672906792E-5	0.26331695008969613	3.81091009360188E-5
122-123	0.128613981314515	3.6415363116640184E-4	1.27030336453396E-5	2.54060672906792E-5	0.2640113825956414	3.81091009360188E-5
124-125	0.12863727020953147	3.6627080344062515E-4	1.27030336453396E-5	2.54060672906792E-5	0.2647227524797804	4.0226273210242066E-5
126-127	0.128652090415451	3.683879757148484E-4	1.27030336453396E-5	2.54060672906792E-5	0.26545529408666163	4.4460617758688596E-5
128-129	0.1286669106213706	3.683879757148484E-4	1.27030336453396E-5	2.54060672906792E-5	0.2662005387271882	4.6577790032911864E-5
130-131	0.1286965510332097	3.768566648117415E-4	1.27030336453396E-5	2.54060672906792E-5	0.26719349252379887	5.08121345813584E-5
132-133	0.1287240742727746	3.768566648117415E-4	1.27030336453396E-5	2.54060672906792E-5	0.26790697958021215	5.71636514040282E-5
134-135	0.12875583185688796	3.768566648117415E-4	1.27030336453396E-5	2.9640411839125734E-5	0.2684997878169947	6.3515168226698E-5
136-137	0.12878758944100133	3.768566648117415E-4	1.27030336453396E-5	3.387475638757227E-5	0.26916034556655233	6.774951277514453E-5
138-139	0.12883205005876	3.7897383708596473E-4	1.27030336453396E-5	3.387475638757227E-5	0.2698865356566109	7.410102959781433E-5
>>END_MODULE
