##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.2d.S.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24134792
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.68097247326598	37.0	35.0	38.0	33.0	39.0
2	35.31611343491173	35.0	34.0	37.0	33.0	39.0
3	35.44758475647936	35.0	34.0	37.0	33.0	39.0
4	35.51361312747174	36.0	35.0	38.0	33.0	39.0
5	35.43343808390808	36.0	35.0	37.0	33.0	39.0
6	35.38563589029481	35.0	34.0	37.0	33.0	39.0
7	35.438732142377695	35.0	34.0	38.0	33.0	39.0
8	35.54577366981244	36.0	35.0	38.0	33.0	39.0
9	35.53790971142407	36.0	35.0	38.0	33.0	39.0
10-14	35.500195087656024	35.8	34.4	37.8	33.0	39.0
15-19	35.47195169529532	36.0	34.0	38.0	33.0	39.0
20-24	35.44550208677995	36.0	34.0	38.0	33.0	39.0
25-29	35.43815685670711	36.0	34.0	38.0	33.0	39.0
30-34	35.421389660205065	36.0	34.0	38.0	33.0	39.0
35-39	35.394017582583686	36.0	34.0	38.0	33.0	39.0
40-44	35.37873398701758	36.0	34.0	38.0	32.6	39.0
45-49	35.34993718611704	36.0	34.0	38.0	32.0	39.0
50-54	35.33149583389822	36.0	34.0	38.0	32.0	39.0
55-59	35.29977026526684	36.0	34.0	38.0	32.0	39.0
60-64	35.2607943834776	36.0	34.0	38.0	32.0	39.0
65-69	35.22221473464532	36.0	34.0	38.0	32.0	39.0
70-74	35.204215565644816	36.0	34.0	38.0	32.0	39.0
75-79	35.14820722714329	36.0	34.0	38.0	32.0	39.0
80-84	35.074727182235506	35.8	34.0	38.0	32.0	39.0
85-89	35.0567502466978	35.8	34.0	38.0	32.0	39.0
90-94	35.009390650642445	35.2	34.0	38.0	31.6	39.0
95-99	34.944570493916004	35.2	34.0	37.2	31.0	39.0
100-104	34.87966332587412	35.0	34.0	37.0	31.0	39.0
105-109	34.82520110386698	35.0	34.0	37.0	31.0	39.0
110-114	34.753232627817965	35.0	34.0	37.0	31.0	39.0
115-119	34.68994193113411	35.0	34.0	37.0	30.4	39.0
120-124	34.61711973320509	35.0	34.0	37.0	30.0	39.0
125-129	34.5372894450468	35.0	34.0	37.0	30.0	39.0
130-134	34.4592551615941	35.0	34.0	37.0	30.0	39.0
135-139	34.373762235033965	35.0	34.0	37.0	30.0	39.0
140-144	34.2756930989917	35.0	34.0	37.0	29.0	39.0
145-149	34.16975245529359	35.0	34.0	37.0	29.0	39.0
150	34.10211453241445	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	19.0
21	188.0
22	1738.0
23	8924.0
24	28958.0
25	72011.0
26	145847.0
27	255863.0
28	379430.0
29	502989.0
30	648541.0
31	848242.0
32	1134907.0
33	1581974.0
34	2361297.0
35	4005976.0
36	1.0002234E7
37	2155645.0
38	9.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.69401733397993	19.03353051478546	14.802534863362402	32.469917287872214
2	24.519959588628716	25.963771552859104	31.896567904814262	17.61970095369792
3	20.123877595464673	29.43625948796244	29.310801601273383	21.129061315299506
4	23.742118846518338	34.0063630960648	21.33154907653648	20.919968980880384
5	25.73534505704462	34.94463511431961	21.290720052611185	18.029299776024587
6	19.448048278186945	39.03591959690392	21.507883722387167	20.008148402521968
7	20.81380688924106	20.74560244811722	36.326619264006915	22.113971398634803
8	21.903242422806045	23.568697008037194	27.600080415029062	26.927980154127702
9	23.776562897248088	23.41945188506286	27.26937526538451	25.53460995230454
10-14	23.37212435889234	28.82552872218663	24.785614891564013	23.01673202735702
15-19	23.470865628342686	27.670377271119634	26.312104119231687	22.546652981305993
20-24	23.325820215425043	27.648258429483295	26.273372358222	22.752548996869656
25-29	23.440585884813807	27.24128160906696	26.2493907239755	23.068741782143736
30-34	23.23960446810563	26.98371877412492	26.495628385776023	23.281048371993425
35-39	23.56308933592633	26.701867577727622	26.556682982807562	23.17836010353849
40-44	23.738630107108445	26.39935326560925	26.567972079477624	23.294044547804678
45-49	23.635863942809202	26.222049893779904	26.66846186202889	23.473624301382003
50-54	23.8307446265188	26.230742955901533	26.37405722306666	23.56445519451301
55-59	23.864875239032514	26.22792854398745	26.44259208863288	23.464604128347162
60-64	23.52765169884207	26.20281624966977	26.50561894214792	23.763913109340244
65-69	23.758886341344894	26.168242096306443	26.532024804688604	23.540846757660063
70-74	23.94003001094876	26.122987313277758	26.253699027144705	23.68328364862878
75-79	23.7144583636768	26.230716220798588	26.560222271648332	23.494603143876276
80-84	24.046085661853812	26.226859397015712	26.24003773046247	23.487017210668007
85-89	23.728498239860503	26.325500154722697	26.506045322554183	23.43995628286262
90-94	23.74443997694283	26.048404311916173	26.567669611571542	23.639486099569453
95-99	23.721845406596795	26.260003618510602	26.60412923056581	23.414021744326792
100-104	23.9970363117279	26.03996255695926	26.54916271911521	23.413838412197627
105-109	23.782426631230138	26.22787219380221	26.488655050352207	23.501046124615453
110-114	23.8747257486205	26.323989864921977	26.381286401805326	23.4199979846522
115-119	23.98801468011835	26.410390662661527	26.535502991163156	23.066091666056966
120-124	23.975402812669774	26.549582030787754	26.634161172799832	22.84085398374264
125-129	23.916642828328495	26.64342249148035	26.487915868510488	22.952018811680663
130-134	24.085447266336498	26.653151185226704	26.5035381286899	22.757863419746897
135-139	23.845436082482085	26.673079262502036	26.781137372138943	22.700347282876933
140-144	23.8937265338769	26.796429817998845	26.589036275928958	22.720807372195292
145-149	24.014745751161755	27.333164769379987	26.128645651743398	22.52344382771486
150	23.766071818642565	27.390768480623322	26.06909560272987	22.774064098004242
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	10.0
3	27.5
4	32.5
5	30.5
6	27.5
7	20.5
8	13.5
9	10.5
10	6.5
11	2.0
12	4.0
13	7.5
14	15.0
15	25.0
16	33.5
17	66.0
18	128.0
19	214.0
20	366.5
21	559.5
22	903.0
23	1515.0
24	2414.0
25	3956.0
26	6253.5
27	9634.0
28	14625.5
29	20924.5
30	29425.5
31	42202.0
32	61004.5
33	84502.5
34	115189.0
35	164348.0
36	234333.0
37	319676.5
38	428663.5
39	565197.0
40	727702.5
41	910018.5
42	1105461.0
43	1296948.5
44	1453091.5
45	1565807.0
46	1615845.5
47	1600442.0
48	1572644.5
49	1548858.0
50	1456518.5
51	1300082.0
52	1116374.0
53	939200.5
54	794189.0
55	673095.5
56	562486.5
57	458838.0
58	380563.5
59	313351.5
60	237272.5
61	158467.0
62	96898.0
63	58498.0
64	36425.0
65	19441.5
66	11303.0
67	6377.5
68	3729.5
69	2418.5
70	1421.5
71	1237.0
72	1144.5
73	934.5
74	722.0
75	413.5
76	138.5
77	44.0
78	14.5
79	3.5
80	1.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.143395973746117E-6
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.02805493413823496
25-29	8.286791947492234E-7
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.286791947492234E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	8.286791947492234E-7
75-79	0.0
80-84	0.05544195284550204
85-89	1.6573583894984469E-6
90-94	0.0
95-99	8.286791947492234E-7
100-104	0.0
105-109	0.0
110-114	0.0
115-119	8.286791947492234E-7
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.02923331595316836
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4134792E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.88860707319861
#Duplication Level	Percentage of total
1	24.776427607920798
2	8.062749255343622
3	5.043228527281041
4	3.5009204356327697
5	2.622318878340547
6	2.154778752778777
7	1.8024482543408205
8	1.4939736610950645
9	1.287979646512347
>10	16.753291729888183
>50	6.164058048805732
>100	13.571209391315204
>500	4.749063456921447
>1k	6.37983582889134
>5k	1.0359001231049145
>10k+	0.6018164018275451
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	26641	0.11038421213657032	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.1075469803095877E-4	0.0	0.0	0.0	0.00248603758424767	0.0
2	0.001802377248579561	1.2430187921238352E-5	0.0	0.0	0.010358489934365294	0.0
3	0.0026061960674863076	1.2430187921238352E-5	0.0	0.0	0.014348580257082804	0.0
4	0.00623166754451416	1.657358389498447E-5	0.0	0.0	0.020580247801596965	0.0
5	0.006476127906965181	1.657358389498447E-5	0.0	4.143395973746117E-6	0.02472364377534308	0.0
6	0.0076569957594828245	1.657358389498447E-5	0.0	4.143395973746117E-6	0.03214860936029612	0.0
7	0.009728693746355883	2.4860375842476704E-5	0.0	4.143395973746117E-6	0.04330677471759442	0.0
8	0.016648165022511898	2.900377181622282E-5	0.0	4.143395973746117E-6	0.05855861529695387	0.0
9	0.01712879895546645	2.900377181622282E-5	0.0	4.143395973746117E-6	0.0695759051911448	0.0
10-11	0.017756523445488986	2.900377181622282E-5	0.0	4.143395973746117E-6	0.11669874760055939	0.0
12-13	0.018589346036211957	2.900377181622282E-5	0.0	4.143395973746117E-6	0.1397443160065353	0.0
14-15	0.021665817546718445	3.5218865776842E-5	0.0	4.143395973746117E-6	0.15034933800133848	0.0
16-17	0.025171130540507663	3.9362261750588117E-5	0.0	4.143395973746117E-6	0.1582280054454167	0.0
18-19	0.032158967850230494	4.972075168495341E-5	0.0	4.143395973746117E-6	0.16522412954708704	0.0
20-21	0.037947292025553817	4.972075168495341E-5	0.0	4.143395973746117E-6	0.16966792172892975	0.0
22-23	0.040342174898379074	4.972075168495341E-5	8.286791947492234E-6	4.143395973746117E-6	0.1743396006893285	0.0
24-25	0.04567886891256406	5.179244967182646E-5	8.286791947492234E-6	4.143395973746117E-6	0.1786363023141032	0.0
26-27	0.05028011014140914	5.386414765869952E-5	8.286791947492234E-6	4.143395973746117E-6	0.18200281154277193	0.0
28-29	0.060829196290566746	5.386414765869952E-5	8.286791947492234E-6	4.143395973746117E-6	0.1851911547445696	0.0
30-31	0.08336098359579813	5.386414765869952E-5	1.0358489934365292E-5	4.143395973746117E-6	0.1886260300068051	0.0
32-33	0.10231702017568661	5.800754363244564E-5	1.2430187921238352E-5	4.143395973746117E-6	0.1911949355105277	0.0
34-35	0.11625333253338169	5.800754363244564E-5	1.2430187921238352E-5	8.286791947492234E-6	0.1940849542022156	0.0
36-37	0.11886367199684174	5.800754363244564E-5	1.2430187921238352E-5	8.286791947492234E-6	0.19597434276624384	0.0
38-39	0.12114875487636273	5.800754363244564E-5	1.2430187921238352E-5	8.286791947492234E-6	0.19740174267919938	0.0
40-41	0.12258236988327888	6.215093960619175E-5	1.2430187921238352E-5	1.0358489934365292E-5	0.19873384448475878	0.0
42-43	0.122841332131638	6.629433557993787E-5	1.2430187921238352E-5	1.2430187921238352E-5	0.20019024816953052	0.0
44-45	0.12364100755457101	8.079622148804929E-5	1.2430187921238352E-5	1.2430187921238352E-5	0.20138768960594317	0.0
46-47	0.12494203389032729	9.115471142241458E-5	1.2430187921238352E-5	1.2430187921238352E-5	0.2027073612235813	2.0716979868730586E-6
48-49	0.1260276036354488	9.52981073961607E-5	1.2430187921238352E-5	1.2430187921238352E-5	0.20378050078078153	4.143395973746117E-6
50-51	0.12699094319934473	9.52981073961607E-5	1.2430187921238352E-5	1.2430187921238352E-5	0.20489300259973237	4.143395973746117E-6
52-53	0.1280392223807025	9.52981073961607E-5	1.2430187921238352E-5	1.2430187921238352E-5	0.2060282930965388	6.215093960619176E-6
54-55	0.1285405732935258	1.0151320135677987E-4	1.2430187921238352E-5	1.2430187921238352E-5	0.20694398360673671	8.286791947492234E-6
56-57	0.12935267890438001	1.0772829531739904E-4	1.2430187921238352E-5	1.2430187921238352E-5	0.2078493156270002	1.0358489934365292E-5
58-59	0.12951219964936928	1.0772829531739904E-4	1.450188590811141E-5	1.450188590811141E-5	0.20868420991571007	1.2430187921238352E-5
60-61	0.1298022373675315	1.0772829531739904E-4	1.657358389498447E-5	1.657358389498447E-5	0.20933886647956196	1.2430187921238352E-5
62-63	0.13005912791790375	1.0979999330427211E-4	1.657358389498447E-5	1.657358389498447E-5	0.21016340227833744	1.2430187921238352E-5
64-65	0.13008398829374623	1.201584832386374E-4	1.657358389498447E-5	1.657358389498447E-5	0.21095479090932295	1.2430187921238352E-5
66-67	0.13016478451523428	1.3258867115987575E-4	1.657358389498447E-5	1.657358389498447E-5	0.21153072294967365	1.2430187921238352E-5
68-69	0.13023729394477485	1.40875463107368E-4	1.657358389498447E-5	1.657358389498447E-5	0.2122185266813155	1.2430187921238352E-5
70-71	0.13039059959580346	1.5123395304173328E-4	1.657358389498447E-5	1.657358389498447E-5	0.21290633041295737	1.2430187921238352E-5
72-73	0.13045067883742276	1.6159244297609857E-4	1.657358389498447E-5	1.657358389498447E-5	0.21340560962779376	1.2430187921238352E-5
74-75	0.13048175430722586	1.6159244297609857E-4	1.657358389498447E-5	1.657358389498447E-5	0.2139649680842495	1.2430187921238352E-5
76-77	0.13050661468306834	1.6366414096297163E-4	2.4860375842476704E-5	1.657358389498447E-5	0.21466934539978633	1.450188590811141E-5
78-79	0.1305646222267008	1.698792349235908E-4	2.4860375842476704E-5	1.657358389498447E-5	0.21575698684289468	1.657358389498447E-5
80-81	0.13062055807234635	1.7195093291046386E-4	2.4860375842476704E-5	1.657358389498447E-5	0.21660016792355202	2.4860375842476704E-5
82-83	0.13065992033409693	1.843811208317022E-4	2.6932073829349764E-5	1.657358389498447E-5	0.21753864711160553	2.6932073829349764E-5
84-85	0.13070342599182128	1.947396107660675E-4	2.900377181622282E-5	1.657358389498447E-5	0.2184874847895934	2.900377181622282E-5
86-87	0.1307448599515587	1.9681130875294056E-4	2.900377181622282E-5	1.657358389498447E-5	0.21933895266219822	2.900377181622282E-5
88-89	0.13077386372337496	1.9888300673981364E-4	2.900377181622282E-5	1.657358389498447E-5	0.22013448468915747	3.314716778996894E-5
90-91	0.1308007957972043	2.0716979868730585E-4	2.900377181622282E-5	1.657358389498447E-5	0.22072906201139003	3.729056376371506E-5
92-93	0.1308235844750599	2.0716979868730585E-4	2.900377181622282E-5	1.657358389498447E-5	0.22123662801817393	3.729056376371506E-5
94-95	0.1308608750388236	2.1131319466105198E-4	2.900377181622282E-5	1.657358389498447E-5	0.22205494872298878	3.729056376371506E-5
96-97	0.13086501843479736	2.154565906347981E-4	2.900377181622282E-5	1.657358389498447E-5	0.2227137486828144	3.729056376371506E-5
98-99	0.13088366371667923	2.154565906347981E-4	3.107546980309588E-5	1.657358389498447E-5	0.22337047694465317	3.729056376371506E-5
100-101	0.1309250976764167	2.2374338258229033E-4	3.314716778996894E-5	1.657358389498447E-5	0.22393190709909577	3.729056376371506E-5
102-103	0.13094374295829855	2.2788677855603646E-4	3.314716778996894E-5	1.657358389498447E-5	0.22449955234749902	3.729056376371506E-5
104-105	0.13098517691803602	2.2788677855603646E-4	3.314716778996894E-5	1.657358389498447E-5	0.22513349193148213	4.764905369808034E-5
106-107	0.13100796559589162	2.3203017452978257E-4	3.314716778996894E-5	2.0716979868730585E-5	0.22570113717988538	5.386414765869952E-5
108-109	0.13104525615965534	2.444603624510209E-4	3.314716778996894E-5	2.0716979868730585E-5	0.22626671073030172	5.386414765869952E-5
110-111	0.13108876181737966	2.48603758424767E-4	3.314716778996894E-5	2.0716979868730585E-5	0.22690065031428486	5.593584564557258E-5
112-113	0.13109083351536654	2.48603758424767E-4	3.314716778996894E-5	2.0716979868730585E-5	0.22760088423384797	5.800754363244564E-5
114-115	0.13110533540127464	2.48603758424767E-4	3.314716778996894E-5	2.0716979868730585E-5	0.22821617853594928	5.800754363244564E-5
116-117	0.1311260523811434	2.48603758424767E-4	3.314716778996894E-5	2.0716979868730585E-5	0.22867816718702194	5.800754363244564E-5
118-119	0.13113226747510398	2.48603758424767E-4	3.314716778996894E-5	2.0716979868730585E-5	0.22924166903945142	5.800754363244564E-5
120-121	0.13114262596503834	2.48603758424767E-4	3.314716778996894E-5	2.0716979868730585E-5	0.22992532937511956	6.629433557993787E-5
122-123	0.13115919954893335	2.527471543985131E-4	3.314716778996894E-5	2.0716979868730585E-5	0.23040596330807409	6.629433557993787E-5
124-125	0.13117577313282833	2.631056443328784E-4	3.314716778996894E-5	2.0716979868730585E-5	0.23093424629472672	7.0437731553684E-5
126-127	0.1312151353945789	2.7346413426724376E-4	3.314716778996894E-5	2.0716979868730585E-5	0.23153089531494617	7.0437731553684E-5
128-129	0.13123585237444763	2.7760753024098987E-4	3.5218865776842E-5	2.0716979868730585E-5	0.23222491414054863	7.872452350117623E-5
130-131	0.13125864105230323	2.858943221884821E-4	3.729056376371506E-5	2.0716979868730585E-5	0.23303080465744225	8.079622148804929E-5
132-133	0.13129178822009324	2.900377181622282E-4	3.729056376371506E-5	2.0716979868730585E-5	0.2336896046172679	8.286791947492234E-5
134-135	0.13131457689794881	2.900377181622282E-4	3.729056376371506E-5	2.0716979868730585E-5	0.23440226872475223	8.286791947492234E-5
136-137	0.13133529387781756	2.9418111413597435E-4	3.729056376371506E-5	2.0716979868730585E-5	0.23507764226847283	8.286791947492234E-5
138-139	0.13136015425366004	2.962528121228474E-4	3.729056376371506E-5	2.0716979868730585E-5	0.23578409128199657	8.701131544866846E-5
>>END_MODULE
