##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.4d.R.1.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24115250
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.80813816983029	36.0	34.0	38.0	30.0	39.0
2	34.68336832502255	35.0	34.0	37.0	31.0	39.0
3	34.83907502513969	35.0	34.0	38.0	31.0	39.0
4	34.81863903546511	35.0	34.0	38.0	31.0	39.0
5	34.69376639263537	35.0	34.0	37.0	31.0	39.0
6	34.69345173697142	35.0	34.0	37.0	31.0	39.0
7	34.73811364178269	35.0	34.0	37.0	32.0	39.0
8	34.852403603528884	35.0	34.0	37.0	32.0	39.0
9	34.81541439545516	35.0	34.0	37.0	31.0	39.0
10-14	34.7962011175501	35.0	34.0	37.0	31.2	39.0
15-19	34.81478340883881	35.0	34.0	37.0	31.0	39.0
20-24	34.801946071469295	35.0	34.0	37.0	31.0	39.0
25-29	34.79636445817481	35.0	34.0	37.0	31.0	39.0
30-34	34.782121504027536	35.0	34.0	37.0	31.0	39.0
35-39	34.77274279138719	35.0	34.0	37.0	31.0	39.0
40-44	34.73042931754803	35.0	34.0	37.0	31.0	39.0
45-49	34.63249573610061	35.0	34.0	37.0	31.0	39.0
50-54	34.605734951949486	35.0	34.0	37.0	31.0	39.0
55-59	34.59150529229429	35.0	34.0	37.0	31.0	39.0
60-64	34.56834882491369	35.0	34.0	37.0	30.4	39.0
65-69	34.53170663376908	35.0	34.0	37.0	30.2	39.0
70-74	34.53270262593172	35.0	34.0	37.0	30.0	39.0
75-79	34.516659566042236	35.0	34.0	37.0	30.0	39.0
80-84	34.50296359357667	35.0	34.0	37.0	30.0	39.0
85-89	34.48369210354444	35.0	34.0	37.0	30.0	39.0
90-94	34.45952843947295	35.0	34.0	37.0	30.0	39.0
95-99	34.44438716579758	35.0	34.0	37.0	30.0	39.0
100-104	34.40154789189413	35.0	34.0	37.0	30.0	39.0
105-109	34.376267556836446	35.0	34.0	37.0	30.0	39.0
110-114	34.33948191289744	35.0	34.0	37.0	30.0	39.0
115-119	34.30203242346648	35.0	34.0	37.0	30.0	39.0
120-124	34.25889387835498	35.0	34.0	37.0	30.0	39.0
125-129	34.24138940297115	35.0	34.0	37.0	30.0	39.0
130-134	34.184267631478	35.0	34.0	37.0	29.2	39.0
135-139	34.147497902779364	35.0	34.0	37.0	29.0	39.0
140-144	34.09352196639056	35.0	34.0	37.0	29.0	39.0
145-149	34.05493629964441	35.0	34.0	37.0	29.0	39.0
150	34.04411586029587	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	22.0
22	367.0
23	3631.0
24	22542.0
25	86713.0
26	216989.0
27	388751.0
28	568430.0
29	740811.0
30	928761.0
31	1166114.0
32	1490088.0
33	1958636.0
34	2694802.0
35	4192798.0
36	8576138.0
37	1079641.0
38	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.011200891188075	10.794119100863421	14.747451651288829	37.44722835665968
2	22.928406654218104	12.646236424035765	36.19527161880687	28.230085302939262
3	21.829084568045428	17.360508629056724	28.01287638214598	32.79753042075187
4	26.81508072324069	24.991776982817004	22.03237405597026	26.160768237972043
5	26.267715043907092	30.714596306285678	24.190762703648208	18.826925946159022
6	19.71145211730261	36.82941509796608	25.37057590357299	18.088556881158322
7	16.25760991930579	25.03139508980961	40.34118927967364	18.369805711210955
8	18.780595549758576	24.641530491663133	31.431181397350084	25.146692561228214
9	20.027044324610518	23.05911626123678	32.571272605946135	24.342566808206566
10-14	20.505190369788984	29.450552514594293	27.00315272287273	23.041104392743996
15-19	20.669358240189982	28.053352978581707	27.824732141482052	23.45255663974626
20-24	20.876095510427756	28.215721163582273	27.515625706763863	23.392557619226103
25-29	20.866153457493926	28.405432797112677	27.40153854936047	23.326875196032923
30-34	20.83150706513979	28.146819298552284	27.433018216249266	23.588655420058657
35-39	20.878027329007143	28.121324806460663	27.493412813296974	23.507235051235224
40-44	20.938095609803092	28.203922730761587	27.384797061279293	23.47318459815603
45-49	21.05277352909934	27.96180943254604	27.35631442448242	23.629102613872195
50-54	21.153707572398137	27.95655126945311	27.430795488676097	23.45894566947265
55-59	21.144113041961628	27.983278777197242	27.383342355297412	23.48926582554372
60-64	21.092065340122762	27.916335015762083	27.34858394221169	23.643015701903465
65-69	21.19172705023851	27.821962990944794	27.452937843365355	23.533372115451343
70-74	21.323874989491067	27.742403179763613	27.431924329688574	23.50179750105674
75-79	21.368628899549062	27.64569515934457	27.4167252182147	23.568950722891667
80-84	21.383904266612006	27.662500985373427	27.39050598868607	23.563088759328497
85-89	21.341923578237292	27.67625581280995	27.46886488357055	23.512955725382213
90-94	21.325458008254405	27.58016592707567	27.41616079113256	23.67821527353737
95-99	21.422691385973426	27.481379131525213	27.529229393247384	23.566700089253974
100-104	21.508206215616546	27.54989120403598	27.366630273625226	23.575272306722248
105-109	21.46802421451058	27.382153272109882	27.484741569622926	23.665080943756607
110-114	21.529904026502606	27.365484154734215	27.54206957784141	23.562542240921765
115-119	21.553222303459926	27.39192854629568	27.51844273610286	23.536406414141535
120-124	21.521426201734712	27.308033892008197	27.51200042422982	23.658539482027265
125-129	21.56736626733994	27.23778352194089	27.570434802022735	23.62441540869644
130-134	21.63230599308242	27.348789084573692	27.464459491745867	23.55444543059802
135-139	21.612985275378094	27.220894491096224	27.565741973148718	23.600378260376967
140-144	21.663954052890137	27.146087251943797	27.687674222812227	23.50228447235384
145-149	21.705212853289016	27.751205041148552	27.301326772081246	23.242255333481186
150	21.56738114731801	27.292011953275008	27.396895870626498	23.74371102878048
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	0.5
10	1.0
11	7.0
12	12.0
13	16.5
14	31.5
15	78.0
16	175.5
17	358.5
18	672.0
19	1213.5
20	2309.0
21	3922.0
22	6120.0
23	9709.0
24	15390.0
25	23995.0
26	35573.5
27	49994.5
28	67619.0
29	93851.5
30	126795.0
31	161491.5
32	194304.0
33	230480.0
34	271228.5
35	318970.0
36	391831.0
37	489368.0
38	607647.0
39	753757.5
40	918896.0
41	1083690.0
42	1252833.5
43	1423186.5
44	1562568.0
45	1645035.5
46	1664841.5
47	1626282.0
48	1540055.5
49	1412919.5
50	1250344.5
51	1075180.0
52	898491.5
53	722728.5
54	560455.5
55	425636.0
56	321072.5
57	240675.0
58	180317.5
59	133099.5
60	93033.0
61	64119.0
62	45590.0
63	31406.5
64	22044.0
65	16986.5
66	14428.5
67	10633.5
68	6450.5
69	4417.5
70	3534.0
71	2740.0
72	2160.0
73	1392.0
74	709.5
75	239.5
76	68.0
77	39.5
78	19.5
79	3.5
80	1.5
81	2.0
82	1.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0781559386695141E-4
2	8.293507220534724E-5
3	0.16139994401882626
4	5.8054550543743064E-5
5	4.976104332320835E-5
6	6.220130415401043E-5
7	5.8054550543743064E-5
8	7.878831859507988E-5
9	9.95220866464167E-5
10-14	1.318667648065021E-4
15-19	1.5674728646810628E-4
20-24	2.189485906221167E-4
25-29	1.683581965768549E-4
30-34	1.832865095738174E-4
35-39	9.703403448025628E-5
40-44	2.040202776251542E-4
45-49	1.6089404007837364E-4
50-54	9.537533303614933E-5
55-59	1.1610910108748613E-4
60-64	1.3933092130498338E-4
65-69	0.06420501549849161
70-74	2.0153222545899377E-4
75-79	1.3352546625060906E-4
80-84	1.1196234747721877E-4
85-89	8.210572148329376E-5
90-94	1.2274390686391393E-4
95-99	8.874052725972154E-5
100-104	7.132416209659862E-5
105-109	9.869273592436322E-5
110-114	3.815013321445973E-5
115-119	4.3126237546780566E-5
120-124	7.961766931713335E-5
125-129	1.0947429531105835E-4
130-134	2.8197924549818063E-5
135-139	3.89794839365132E-5
140-144	3.980883465856668E-5
145-149	6.386000559811738E-5
150	7.464156498481252E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.411525E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.70173341314269
#Duplication Level	Percentage of total
1	41.56286031741834
2	10.133567394641723
3	6.29203071357334
4	4.501574079253584
5	3.3488933948130137
6	2.599706032129473
7	2.1322686496302357
8	1.8630302307610092
9	1.5139161761788942
>10	17.32975114453632
>50	4.1143367590578634
>100	3.958412143586614
>500	0.33974591893887995
>1k	0.30990704548069903
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	2.9027275271871532E-5	0.0	8.293507220534723E-6	1.4928312996962504E-4	4.561428971294098E-5
2	4.146753610267362E-6	9.122857942588196E-5	0.0	1.2440260830802086E-5	0.0019780014720975317	9.122857942588196E-5
3	8.293507220534723E-6	1.0781559386695141E-4	0.0	1.6587014441069446E-5	0.0031225054685313234	1.5757663719015975E-4
4	2.488052166160417E-5	1.202558546977535E-4	0.0	1.6587014441069446E-5	0.004631923782668643	1.617233908004271E-4
5	2.9027275271871532E-5	1.2440260830802086E-4	0.0	1.6587014441069446E-5	0.0059174174018515255	1.7001689802096184E-4
6	4.561428971294098E-5	1.2440260830802086E-4	0.0	1.6587014441069446E-5	0.007128269456049595	1.741636516312292E-4
7	5.3907796933475704E-5	1.409896227490903E-4	0.0	1.6587014441069446E-5	0.008272773452483386	1.741636516312292E-4
8	6.634805776427779E-5	1.4928312996962504E-4	0.0	1.6587014441069446E-5	0.010478846373145624	1.8245715885176393E-4
9	8.70818258156146E-5	1.534298835798924E-4	0.0	1.6587014441069446E-5	0.014074081753247427	1.9075066607229864E-4
10-11	1.4720975316449134E-4	1.6172339080042713E-4	0.0	1.6587014441069446E-5	0.01882418801380869	2.46731839810908E-4
12-13	2.5087859342117543E-4	1.7209027482609552E-4	0.0	1.6587014441069446E-5	0.022821658494106424	2.9441950632898273E-4
14-15	2.612454774468438E-4	1.8660391246203128E-4	0.0	1.6587014441069446E-5	0.02729600563958491	3.504006800675921E-4
16-17	3.151532743803195E-4	2.0319092690310073E-4	0.0	2.073376805133681E-5	0.03181182032116607	3.607675640932605E-4
18-19	3.566208104829931E-4	2.1148443412363546E-4	0.0	2.073376805133681E-5	0.03572220897564819	3.6284094089839415E-4
20-21	4.4162925949347404E-4	2.5709872383657644E-4	0.0	2.073376805133681E-5	0.039479167746550414	3.773545785343299E-4
22-23	5.287110853090886E-4	2.6331885425197744E-4	0.0	2.6953898466737852E-5	0.0435657934294689	3.8979483936513203E-4
24-25	6.406734327863075E-4	2.7161236147251226E-4	0.0	2.9027275271871532E-5	0.04801940680689605	3.8979483936513203E-4
26-27	7.650760410943283E-4	2.7575911508277956E-4	0.0	2.9027275271871532E-5	0.052244948735758495	4.208954914421372E-4
28-29	8.480111132996755E-4	2.7783249188791326E-4	0.0	3.524740568727258E-5	0.056673681591524035	4.478493899088751E-4
30-31	9.475331999460922E-4	2.7783249188791326E-4	0.0	4.561428971294098E-5	0.06092410404204808	4.7687666518074663E-4
32-33	0.00101388125771037	2.819792454981806E-4	0.0	5.183442012834202E-5	0.06502109660899225	5.328578389193559E-4
34-35	0.001071935808254113	2.819792454981806E-4	0.0	5.8054550543743064E-5	0.0687676884958688	5.598117373860939E-4
36-37	0.0011113299675516528	2.819792454981806E-4	0.0	7.049481137454515E-5	0.07237329075999627	5.743253750220296E-4
38-39	0.0011445039964337919	2.8819937591358167E-4	0.0	7.878831859507988E-5	0.07671079503633593	5.846922590476979E-4
40-41	0.001202558546977535	2.902727527187153E-4	0.0	7.878831859507988E-5	0.08038067198142254	5.888390126579654E-4
42-43	0.0012212189382237381	2.9234612952384897E-4	0.0	7.878831859507988E-5	0.08374576253615451	6.095727807093022E-4
44-45	0.0012854936191828822	3.0271301354951744E-4	0.0	7.878831859507988E-5	0.08780543432060625	6.157929111247032E-4
46-47	0.0013580618073625612	3.047863903546511E-4	0.0	7.878831859507988E-5	0.09167642881579084	6.344533023709064E-4
48-49	0.0014098962274909032	3.089331439649185E-4	0.0	7.878831859507988E-5	0.09568633955691938	6.572604472273769E-4
50-51	0.0014327033723473734	3.1100652077005215E-4	0.0	7.878831859507988E-5	0.09988492758731508	6.7592083847358E-4
52-53	0.0015425923430194585	3.1100652077005215E-4	0.0	7.878831859507988E-5	0.10395911300940276	6.7592083847358E-4
54-55	0.0016545546904966776	3.172266511854532E-4	0.0	7.878831859507988E-5	0.10719772757902157	6.7592083847358E-4
56-57	0.0016939488497942174	3.3174028882138897E-4	0.0	8.293507220534724E-5	0.11109982272628316	6.987279833300505E-4
58-59	0.0017374897627020246	3.524740568727258E-4	0.0	8.915520262074827E-5	0.11496252371424721	7.21535128186521E-4
60-61	0.0018079845740765698	3.5662081048299315E-4	0.0	9.537533303614932E-5	0.11832761426897917	7.256818817967883E-4
62-63	0.001857745617399778	3.607675640932605E-4	0.0	9.537533303614932E-5	0.12177356651911135	7.298286354070557E-4
64-65	0.001903359907112719	3.6284094089839415E-4	0.0	1.0366884025668405E-4	0.1260302091000508	7.298286354070557E-4
66-67	0.0019282404287743232	3.773545785343299E-4	0.0	1.0366884025668405E-4	0.12995925814577913	7.298286354070557E-4
68-69	0.002040202776251542	3.8979483936513203E-4	0.0	1.0366884025668405E-4	0.13296358113641782	7.360487658224567E-4
70-71	0.00216875213816983	4.022351001959341E-4	0.0	1.1196234747721877E-4	0.13568177812794807	7.401955194327242E-4
72-73	0.002241320326349509	4.1882211463700357E-4	0.0	1.1196234747721877E-4	0.1386985413794176	7.464156498481252E-4
74-75	0.0023035216305035194	4.208954914421372E-4	0.0	1.223292315028872E-4	0.14188532152890804	7.547091570686598E-4
76-77	0.002359502804242129	4.271156218575383E-4	0.0	1.2647598511315454E-4	0.1443319061589658	7.547091570686598E-4
78-79	0.0024258508620064066	4.4162925949347404E-4	0.0	1.43062999554224E-4	0.14687593949886485	7.547091570686598E-4
80-81	0.002471465151719348	4.478493899088751E-4	0.0	1.5135650677475872E-4	0.14944485336042546	7.567825338737936E-4
82-83	0.002527446325457957	4.561428971294098E-4	0.0	1.534298835798924E-4	0.1521381698302941	7.588559106789272E-4
84-85	0.0026497755569608442	4.6236302754481087E-4	0.0	1.534298835798924E-4	0.15461378173562373	7.588559106789272E-4
86-87	0.0027783249188791325	4.644364043499445E-4	0.0	1.5757663719015975E-4	0.15686132219238863	7.609292874840609E-4
88-89	0.0028550398606690787	4.644364043499445E-4	0.0	1.617233908004271E-4	0.1590653217362457	7.630026642891945E-4
90-91	0.00288406713594095	4.665097811550782E-4	0.0	1.617233908004271E-4	0.161907921336084	7.67149417899462E-4
92-93	0.002931754802459025	4.706565347653456E-4	0.0	1.617233908004271E-4	0.16480857548646605	7.67149417899462E-4
94-95	0.003000176237028436	4.727299115704793E-4	0.0	1.617233908004271E-4	0.16783985237557147	7.67149417899462E-4
96-97	0.003097624946869719	4.7687666518074663E-4	0.0	1.617233908004271E-4	0.1703776655850551	7.67149417899462E-4
98-99	0.0032386145696188095	4.8517017240128134E-4	0.0	1.6587014441069449E-4	0.17244482225977337	7.67149417899462E-4
100-101	0.003358870424316563	4.93463679621816E-4	0.0	1.7001689802096184E-4	0.17439379645659903	7.67149417899462E-4
102-103	0.003385824322783301	4.93463679621816E-4	0.0	1.7209027482609552E-4	0.17619763427706533	7.67149417899462E-4
104-105	0.003431438612496242	5.017571868423508E-4	0.0	1.7831040524149657E-4	0.17797866495267517	7.67149417899462E-4
106-107	0.0035123003078964556	5.141974476731529E-4	0.0	1.7831040524149657E-4	0.17971822809218232	7.692227947045957E-4
108-109	0.003618042524958273	5.141974476731529E-4	0.0	1.7831040524149657E-4	0.1815324327966743	7.712961715097294E-4
110-111	0.003723784742020091	5.183442012834203E-4	0.0	1.7831040524149657E-4	0.18314551995106831	7.712961715097294E-4
112-113	0.003815013321445973	5.245643316988213E-4	0.0	1.7831040524149657E-4	0.18488508309057547	7.712961715097294E-4
114-115	0.0038855081328205182	5.307844621142223E-4	0.0	1.845305356568976E-4	0.1867635624760266	7.712961715097294E-4
116-117	0.003974663335441267	5.328578389193559E-4	0.0	1.9904417329283337E-4	0.18859020744134936	7.712961715097294E-4
118-119	0.0040741854220876835	5.494448533604255E-4	0.0	1.9904417329283337E-4	0.1900768186106302	7.712961715097294E-4
120-121	0.004223468552057308	5.515182301655591E-4	0.0	1.9904417329283337E-4	0.19148878821492624	7.712961715097294E-4
122-123	0.004422512725350141	5.535916069706929E-4	0.0	2.0319092690310073E-4	0.1929380786017147	7.712961715097294E-4
124-125	0.004563502348099232	5.66031867801495E-4	0.0	2.0526430370823443E-4	0.1944101761333596	7.712961715097294E-4
126-127	0.004607043261007039	5.701786214117623E-4	0.0	2.073376805133681E-4	0.19581177885362996	7.712961715097294E-4
128-129	0.004669244565161049	5.763987518271633E-4	0.0	2.073376805133681E-4	0.19733156405179295	7.754429251199967E-4
130-131	0.004764619898197198	5.805455054374306E-4	0.0	2.1148443412363546E-4	0.1987995148298276	7.754429251199967E-4
132-133	0.004849628347207679	5.909123894630991E-4	0.0	2.280714485647049E-4	0.1999315785654306	7.754429251199967E-4
134-135	0.004965737448295166	5.971325198785002E-4	0.0	2.3221820217497226E-4	0.20100766112729496	7.754429251199967E-4
136-137	0.0051710017520034	5.971325198785002E-4	0.0	2.3221820217497226E-4	0.2020692300515234	7.775163019251303E-4
138-139	0.005280890722675485	6.095727807093022E-4	0.0	2.3221820217497226E-4	0.2031453126133878	7.795896787302641E-4
>>END_MODULE
