##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.4d.R.1.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24115250
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.55777675951939	36.0	35.0	38.0	32.0	39.0
2	35.20505427063787	35.0	34.0	37.0	32.0	39.0
3	35.364814256538914	35.0	34.0	37.0	33.0	39.0
4	35.422129357978875	36.0	35.0	37.0	33.0	39.0
5	35.33168007795897	35.0	34.0	37.0	33.0	39.0
6	35.302083370481334	35.0	34.0	37.0	33.0	39.0
7	35.324285421050995	35.0	34.0	37.0	33.0	39.0
8	35.4639955215061	36.0	34.0	38.0	33.0	39.0
9	35.43465417111579	36.0	34.0	38.0	33.0	39.0
10-14	35.4008225085786	35.4	34.2	37.4	33.0	39.0
15-19	35.372704375861744	35.8	34.0	37.4	33.0	39.0
20-24	35.33658480836815	35.6	34.0	37.2	32.8	39.0
25-29	35.325732953214256	35.8	34.0	37.2	32.8	39.0
30-34	35.30726349509128	35.6	34.0	37.0	32.0	39.0
35-39	35.28228993686567	35.4	34.0	37.0	32.0	39.0
40-44	35.257307388478246	35.4	34.0	37.0	32.0	39.0
45-49	35.22773701288604	35.0	34.0	37.0	32.0	39.0
50-54	35.20055817791647	35.0	34.0	37.0	32.0	39.0
55-59	35.16873895978686	35.0	34.0	37.0	32.0	39.0
60-64	35.125391592457056	35.0	34.0	37.0	32.0	39.0
65-69	35.08726984791781	35.0	34.0	37.0	32.0	39.0
70-74	35.06189460196349	35.0	34.0	37.0	32.0	39.0
75-79	35.00851207430982	35.0	34.0	37.0	32.0	39.0
80-84	34.92851647816216	35.0	34.0	37.0	31.2	39.0
85-89	34.90968838390645	35.0	34.0	37.0	31.0	39.0
90-94	34.856167213692586	35.0	34.0	37.0	31.0	39.0
95-99	34.78642994785457	35.0	34.0	37.0	31.0	39.0
100-104	34.71605946444677	35.0	34.0	37.0	31.0	39.0
105-109	34.656107085765235	35.0	34.0	37.0	30.4	39.0
110-114	34.586999686920095	35.0	34.0	37.0	30.0	39.0
115-119	34.52354581437057	35.0	34.0	37.0	30.0	39.0
120-124	34.44596440012025	35.0	34.0	37.0	30.0	39.0
125-129	34.36004645193394	35.0	34.0	37.0	30.0	39.0
130-134	34.28055669337867	35.0	34.0	37.0	29.2	39.0
135-139	34.18732801028395	35.0	34.0	37.0	29.0	39.0
140-144	34.080690160790375	35.0	34.0	37.0	29.0	39.0
145-149	33.970305437430675	35.0	34.0	37.0	28.0	39.0
150	33.898876893252194	35.0	33.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	31.0
21	318.0
22	2300.0
23	11350.0
24	35857.0
25	85208.0
26	166198.0
27	282071.0
28	410360.0
29	542048.0
30	696476.0
31	902783.0
32	1204941.0
33	1669589.0
34	2479643.0
35	4190667.0
36	1.0022338E7
37	1413062.0
38	8.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.39534527678692	18.48525126033335	16.227812221869424	31.891591241010303
2	23.94923228866515	25.18008418656594	33.44771600741091	17.422967517358
3	19.87777858409098	29.012774074496427	30.248494210095274	20.86095313131732
4	23.434718694601962	33.6274224816247	22.495479001876404	20.44237982189693
5	25.038629083256446	34.83649143177035	22.77256507811447	17.352314406858728
6	18.778652512414343	38.75407470376629	23.00023014482537	19.467042638994
7	20.008193985133886	20.46361534713511	38.472962129772654	21.055228537958346
8	21.319662868931484	23.14789604088699	28.59885756938037	26.93358352080115
9	23.238083785156697	22.91216553840412	28.987458143705748	24.862292532733438
10-14	22.837328436050452	28.986624886630864	25.875764305787953	22.30028237153073
15-19	22.66208726842973	28.10337690880252	27.333347155845367	21.901188666922383
20-24	22.75651342786381	28.2137359803141	27.204416989203178	21.825333602618908
25-29	22.869350307378113	28.155855734441896	27.148004685831577	21.82678927234841
30-34	22.86049284166658	27.950731591005688	27.288967769357562	21.899807797970166
35-39	22.94386746975462	27.940368024382913	27.25996205720447	21.855802448658007
40-44	23.07562724831797	27.730207234011672	27.367327313629342	21.82683820404101
45-49	23.082611402184188	27.57270380835947	27.450788421855783	21.893896367600554
50-54	23.16169867187855	27.548622408192884	27.416721636725015	21.872957283203547
55-59	23.30503643959735	27.44802479758659	27.308135723245663	21.938803039570395
60-64	23.237481676532486	27.421993966473497	27.366204372751685	21.974319984242335
65-69	23.311804134825923	27.339044192691713	27.335944909017694	22.01320676346467
70-74	23.376150775961268	27.308934387991002	27.24867210582515	22.066242730222577
75-79	23.294710193757062	27.342654129648253	27.340506940628856	22.022128735965833
80-84	23.396862431686856	27.398789563872484	27.2121079696272	21.99224003481346
85-89	23.435093509214077	27.321633483265025	27.264390035828452	21.97888297169245
90-94	23.37372326639782	27.242704098029257	27.334048786556224	22.0495238490167
95-99	23.45298431490447	27.364194855952146	27.326232985351595	21.856587843791793
100-104	23.604540695203244	27.33009195426131	27.136561304568684	21.928806045966766
105-109	23.383055950073086	27.46963684805258	27.224134106011753	21.923173095862577
110-114	23.41124060501135	27.44926467691606	27.164541109878606	21.974953608193985
115-119	23.497264374180357	27.460692528260687	27.178537458069375	21.86350563948958
120-124	23.4594200765076	27.382398274950496	27.36228983734359	21.79589181119831
125-129	23.451960896113455	27.451707944143227	27.317898839945677	21.778432319797638
130-134	23.48696779009133	27.368691595567118	27.393204714858854	21.751135899482694
135-139	23.410826759001047	27.378548428898725	27.475032603850263	21.735592208249965
140-144	23.423152763692293	27.515267123627307	27.43284044273097	21.62873966994943
145-149	23.548806981096103	28.098978341092668	26.899364615613823	21.452850062197406
150	23.193456422803	28.007650760410947	26.999106374596987	21.79978644218907
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.5
2	15.0
3	28.5
4	37.0
5	41.5
6	28.5
7	17.5
8	15.5
9	13.5
10	11.0
11	11.5
12	16.0
13	17.5
14	30.0
15	47.0
16	71.0
17	132.5
18	237.0
19	395.0
20	635.0
21	1017.0
22	1507.5
23	2549.0
24	4382.5
25	7218.5
26	11509.0
27	18339.0
28	30521.5
29	47232.0
30	63727.0
31	82753.5
32	108358.0
33	141897.0
34	190785.0
35	260916.0
36	360229.5
37	484922.5
38	630951.0
39	812364.0
40	1020118.5
41	1220512.0
42	1403406.5
43	1566971.5
44	1681174.5
45	1733424.0
46	1731681.5
47	1674293.5
48	1568694.5
49	1422275.0
50	1237642.0
51	1041765.5
52	854445.5
53	682327.0
54	533884.5
55	405168.5
56	303145.0
57	225777.5
58	164018.5
59	117474.0
60	80881.0
61	53595.5
62	36218.5
63	25746.5
64	18062.5
65	12622.0
66	9439.5
67	7114.0
68	6102.0
69	4667.0
70	2813.0
71	2016.5
72	1539.5
73	1238.5
74	1019.5
75	615.5
76	240.0
77	101.0
78	28.0
79	4.0
80	3.0
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.2440260830802087E-5
2	4.146753610267362E-6
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	8.293507220534724E-7
15-19	0.0
20-24	0.03319559200091229
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	8.293507220534724E-7
50-54	2.4880521661604173E-6
55-59	0.0
60-64	0.0
65-69	8.293507220534724E-7
70-74	0.0
75-79	0.0
80-84	0.0647134074911104
85-89	3.3174028882138895E-6
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	8.293507220534724E-7
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	8.293507220534724E-7
145-149	0.03506826593130903
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.411525E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.23317214317567
#Duplication Level	Percentage of total
1	30.043360210806007
2	10.35012376325397
3	6.8793902163341425
4	5.209647476077819
5	3.9171504603136333
6	3.1010971090903774
7	2.6198951101731356
8	2.165362197838289
9	1.9245547398302916
>10	20.965846198016084
>50	5.1050359164664805
>100	5.896766515742536
>500	0.9380665994450156
>1k	0.778925175835836
>5k	0.05667254545141
>10k+	0.04810576532503407
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	8.293507220534723E-6	4.146753610267362E-6	0.0	0.0	0.001339401416116358	0.0
2	1.6587014441069446E-5	3.317402888213889E-5	0.0	4.146753610267362E-6	0.005415660215009175	0.0
3	3.317402888213889E-5	3.317402888213889E-5	0.0	4.146753610267362E-6	0.006974839572469703	0.0
4	8.70818258156146E-5	4.146753610267362E-5	0.0	4.146753610267362E-6	0.008326681249416863	0.0
5	8.70818258156146E-5	4.561428971294098E-5	0.0	8.293507220534723E-6	0.009952208664641669	0.0
6	9.537533303614932E-5	4.561428971294098E-5	0.0	8.293507220534723E-6	0.011453333471558453	0.0
7	9.537533303614932E-5	6.634805776427779E-5	0.0	8.293507220534723E-6	0.013443775204486787	0.0
8	1.3684286913882295E-4	7.049481137454515E-5	0.0	8.293507220534723E-6	0.019435834171323125	0.0
9	1.409896227490903E-4	7.464156498481252E-5	0.0	8.293507220534723E-6	0.025324224297902778	0.0
10-11	1.6172339080042713E-4	8.293507220534724E-5	0.0	8.293507220534723E-6	0.031368117684867455	0.0
12-13	1.9489741968256602E-4	8.70818258156146E-5	0.0	8.293507220534723E-6	0.037314562361990856	0.0
14-15	2.177045645390365E-4	1.0366884025668405E-4	0.0	8.293507220534723E-6	0.0451166792797089	0.0
16-17	2.4258508620064067E-4	1.0781559386695142E-4	0.0	8.293507220534723E-6	0.05059454079887209	0.0
18-19	3.0271301354951744E-4	1.202558546977535E-4	0.0	8.293507220534723E-6	0.057870020008086165	0.0
20-21	3.400337960419237E-4	1.3476949233368925E-4	0.0	8.293507220534723E-6	0.06388488611977898	0.0
22-23	3.794279553394636E-4	1.534298835798924E-4	2.073376805133681E-6	1.0366884025668405E-5	0.0704056561719244	0.0
24-25	4.271156218575383E-4	1.617233908004271E-4	4.146753610267362E-6	1.6587014441069446E-5	0.07547091570686598	0.0
26-27	4.561428971294098E-4	1.7209027482609552E-4	4.146753610267362E-6	1.6587014441069446E-5	0.079472532940774	0.0
28-29	5.763987518271633E-4	1.7831040524149655E-4	4.146753610267362E-6	2.073376805133681E-5	0.08380174370989311	0.0
30-31	7.484890266532588E-4	1.8660391246203128E-4	1.0366884025668405E-5	2.073376805133681E-5	0.08869491297000862	0.0
32-33	8.687448813510123E-4	2.0111755009796705E-4	1.2440260830802086E-5	2.073376805133681E-5	0.09362125625900623	0.0
34-35	9.7448709841283E-4	2.0526430370823443E-4	1.2440260830802086E-5	2.073376805133681E-5	0.09696353966888173	0.0
36-37	0.0010304682721514395	2.073376805133681E-4	1.2440260830802086E-5	2.073376805133681E-5	0.10030582307875721	0.0
38-39	0.001078155938669514	2.0941105731850178E-4	1.4513637635935766E-5	2.073376805133681E-5	0.10360041882211463	0.0
40-41	0.0011071832139413857	2.2185131814930387E-4	1.6587014441069446E-5	2.073376805133681E-5	0.10641813790029131	0.0
42-43	0.001136210489213257	2.280714485647049E-4	1.6587014441069446E-5	2.073376805133681E-5	0.10924829723929878	0.0
44-45	0.0011610910108748613	2.4880521661604173E-4	1.6587014441069446E-5	2.073376805133681E-5	0.11182550460807995	0.0
46-47	0.0012004851701724012	2.612454774468438E-4	1.6587014441069446E-5	2.073376805133681E-5	0.11447735354184593	0.0
48-49	0.0012481728366904759	2.7161236147251226E-4	1.6587014441069446E-5	2.073376805133681E-5	0.117270192098361	0.0
50-51	0.0013165942712598873	2.7575911508277956E-4	1.6587014441069446E-5	2.073376805133681E-5	0.11958822736650045	0.0
52-53	0.0013746488218036304	2.7783249188791326E-4	2.488052166160417E-5	2.073376805133681E-5	0.12205761914141466	0.0
54-55	0.0014119696042960367	2.944195063289827E-4	2.9027275271871532E-5	2.073376805133681E-5	0.12433418687345144	0.0
56-57	0.0014389235027627745	3.047863903546511E-4	2.9027275271871532E-5	2.073376805133681E-5	0.12645110459149295	0.0
58-59	0.0014866111692808492	3.234467816008542E-4	2.9027275271871532E-5	2.073376805133681E-5	0.1284000787883186	0.0
60-61	0.0015446657198245923	3.524740568727258E-4	2.9027275271871532E-5	2.073376805133681E-5	0.12992193736328672	0.0
62-63	0.0015882066327323997	3.7320782492406256E-4	2.9027275271871532E-5	2.073376805133681E-5	0.13203056157410767	0.0
64-65	0.0016089404007837364	3.856480857548646E-4	2.9027275271871532E-5	2.280714485647049E-5	0.13380329874249697	0.0
66-67	0.0017457832699225594	4.1052860741646886E-4	2.9027275271871532E-5	2.488052166160417E-5	0.13517587418749547	0.0
68-69	0.001799691066856035	4.208954914421372E-4	2.9027275271871532E-5	2.488052166160417E-5	0.13654637625568883	2.073376805133681E-6
70-71	0.0018142047044919709	4.291889986626719E-4	2.9027275271871532E-5	2.488052166160417E-5	0.13807030820746208	8.293507220534723E-6
72-73	0.0018535988637895108	4.4370263629860774E-4	2.9027275271871532E-5	2.488052166160417E-5	0.139092482972393	1.6587014441069446E-5
74-75	0.0018660391246203129	4.665097811550782E-4	3.317402888213889E-5	2.488052166160417E-5	0.1402950415193705	1.6587014441069446E-5
76-77	0.0019158001679435211	4.93463679621816E-4	3.317402888213889E-5	2.488052166160417E-5	0.14170493774686144	1.6587014441069446E-5
78-79	0.0019780014720975317	5.204175780885539E-4	3.732078249240626E-5	2.6953898466737852E-5	0.14341132685748645	1.6587014441069446E-5
80-81	0.0019945884865386013	5.287110853090886E-4	3.732078249240626E-5	2.9027275271871532E-5	0.14495806595411617	1.6587014441069446E-5
82-83	0.002021542385005339	5.515182301655591E-4	3.732078249240626E-5	2.9027275271871532E-5	0.14667067519515659	1.6587014441069446E-5
84-85	0.002048496283472077	5.577383605809602E-4	3.732078249240626E-5	2.9027275271871532E-5	0.14830656949440707	1.6587014441069446E-5
86-87	0.0020754501819388147	5.681052446066286E-4	3.732078249240626E-5	2.9027275271871532E-5	0.15025761706803786	2.073376805133681E-5
88-89	0.0020837436891593493	5.763987518271633E-4	3.732078249240626E-5	2.9027275271871532E-5	0.15222525165610973	2.280714485647049E-5
90-91	0.0020878904427696168	5.90912389463099E-4	3.9394159297539944E-5	2.9027275271871532E-5	0.15332414136283057	2.488052166160417E-5
92-93	0.0021044774572106864	5.992058966836338E-4	4.561428971294098E-5	3.317402888213889E-5	0.15440022392469493	2.488052166160417E-5
94-95	0.0021210644716517556	6.116461575144359E-4	5.3907796933475704E-5	3.317402888213889E-5	0.15547008635614393	2.488052166160417E-5
96-97	0.002123137848456889	6.261597951503716E-4	5.3907796933475704E-5	3.317402888213889E-5	0.15648811436746457	2.9027275271871532E-5
98-99	0.0021335047324825577	6.427468095914411E-4	5.3907796933475704E-5	3.317402888213889E-5	0.15745430795865686	2.9027275271871532E-5
100-101	0.002143871616508226	6.427468095914411E-4	5.3907796933475704E-5	3.317402888213889E-5	0.15846196908595184	3.317402888213889E-5
102-103	0.002152165123728761	6.427468095914411E-4	6.220130415401043E-5	3.317402888213889E-5	0.15937425488021065	3.732078249240626E-5
104-105	0.002158385254144162	6.427468095914411E-4	6.220130415401043E-5	3.524740568727258E-5	0.16028654067446949	3.732078249240626E-5
106-107	0.00216875213816983	6.531136936171095E-4	6.220130415401043E-5	3.732078249240626E-5	0.16119053296150776	3.732078249240626E-5
108-109	0.00216875213816983	6.717740848633125E-4	6.634805776427779E-5	3.732078249240626E-5	0.16213391940784358	4.146753610267362E-5
110-111	0.002183265775805766	6.821409688889811E-4	6.634805776427779E-5	3.732078249240626E-5	0.16307315910056913	4.561428971294098E-5
112-113	0.0021936326598314343	6.862877224992484E-4	6.634805776427779E-5	3.732078249240626E-5	0.1638589689097148	4.561428971294098E-5
114-115	0.002197779413441702	6.945812297197831E-4	6.634805776427779E-5	3.732078249240626E-5	0.16471734690704015	4.561428971294098E-5
116-117	0.0022081462974673704	6.966546065249168E-4	6.634805776427779E-5	3.732078249240626E-5	0.16571256777350432	5.183442012834202E-5
118-119	0.0022143664278827715	7.070214905505852E-4	7.049481137454515E-5	3.732078249240626E-5	0.16653155161153213	5.3907796933475704E-5
120-121	0.0022143664278827715	7.132416209659863E-4	7.049481137454515E-5	3.732078249240626E-5	0.1674044432464934	5.598117373860939E-5
122-123	0.002216439804687905	7.132416209659863E-4	7.049481137454515E-5	3.732078249240626E-5	0.16816122578036719	5.8054550543743064E-5
124-125	0.0022268066887135736	7.132416209659863E-4	7.049481137454515E-5	3.732078249240626E-5	0.1690776583282363	5.8054550543743064E-5
126-127	0.00224754045676491	7.1531499777112E-4	7.049481137454515E-5	3.732078249240626E-5	0.17013922725246472	5.8054550543743064E-5
128-129	0.002262054094400846	7.236085049916547E-4	7.049481137454515E-5	3.732078249240626E-5	0.17111993448129295	5.8054550543743064E-5
130-131	0.002276567732036782	7.319020122121894E-4	7.049481137454515E-5	3.732078249240626E-5	0.17206124755082364	5.8054550543743064E-5
132-133	0.0022910813696727173	7.381221426275904E-4	7.049481137454515E-5	3.732078249240626E-5	0.1728657177512155	5.8054550543743064E-5
134-135	0.0023055950073086534	7.422688962378578E-4	7.049481137454515E-5	3.9394159297539944E-5	0.1737012886036844	5.8054550543743064E-5
136-137	0.002320108644944589	7.464156498481251E-4	7.049481137454515E-5	4.146753610267362E-5	0.17454722634017894	6.842143456941148E-5
138-139	0.002332548905775391	7.505624034583925E-4	7.256818817967883E-5	4.146753610267362E-5	0.175432558235971	7.049481137454515E-5
>>END_MODULE
