##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.4d.R.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24119721
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.459075791133735	36.0	35.0	38.0	32.0	39.0
2	35.110353059224856	35.0	34.0	37.0	32.0	39.0
3	35.273108590269345	35.0	34.0	37.0	33.0	39.0
4	35.333138637880595	36.0	34.0	37.0	33.0	39.0
5	35.24421439203215	35.0	34.0	37.0	32.0	39.0
6	35.211121554847175	35.0	34.0	37.0	33.0	39.0
7	35.248345824564055	35.0	34.0	37.0	33.0	39.0
8	35.38212266219829	36.0	34.0	38.0	33.0	39.0
9	35.354695230512824	35.0	34.0	38.0	32.0	39.0
10-14	35.31514735182882	35.4	34.0	37.4	32.4	39.0
15-19	35.28445130024514	35.0	34.0	37.0	32.0	39.0
20-24	35.249410911510964	35.0	34.0	37.0	32.0	39.0
25-29	35.23753963820726	35.0	34.0	37.0	32.0	39.0
30-34	35.220386040120445	35.0	34.0	37.0	32.0	39.0
35-39	35.19267127509476	35.0	34.0	37.0	32.0	39.0
40-44	35.169573097466596	35.0	34.0	37.0	32.0	39.0
45-49	35.13905555541045	35.0	34.0	37.0	32.0	39.0
50-54	35.1135459153943	35.0	34.0	37.0	32.0	39.0
55-59	35.080965712663094	35.0	34.0	37.0	32.0	39.0
60-64	35.038146917205225	35.0	34.0	37.0	32.0	39.0
65-69	34.999478741897555	35.0	34.0	37.0	32.0	39.0
70-74	34.9744092479345	35.0	34.0	37.0	31.8	39.0
75-79	34.92334229736737	35.0	34.0	37.0	31.0	39.0
80-84	34.842184078331584	35.0	34.0	37.0	31.0	39.0
85-89	34.82708727849713	35.0	34.0	37.0	31.0	39.0
90-94	34.77289404798671	35.0	34.0	37.0	31.0	39.0
95-99	34.704619676156284	35.0	34.0	37.0	31.0	39.0
100-104	34.63332385146578	35.0	34.0	37.0	30.2	39.0
105-109	34.574445450674986	35.0	34.0	37.0	30.0	39.0
110-114	34.50471393926986	35.0	34.0	37.0	30.0	39.0
115-119	34.44460020909861	35.0	34.0	37.0	30.0	39.0
120-124	34.36739070903847	35.0	34.0	37.0	30.0	39.0
125-129	34.281830498785624	35.0	34.0	37.0	29.6	39.0
130-134	34.20143032334413	35.0	34.0	37.0	29.0	39.0
135-139	34.107813958544554	35.0	34.0	37.0	29.0	39.0
140-144	34.002915274185796	35.0	34.0	37.0	28.4	39.0
145-149	33.89501615711061	35.0	33.4	37.0	28.0	39.0
150	33.828273220905004	35.0	33.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	38.0
21	338.0
22	2740.0
23	12759.0
24	39792.0
25	94795.0
26	184845.0
27	312840.0
28	448436.0
29	579892.0
30	735587.0
31	945368.0
32	1243649.0
33	1703942.0
34	2510485.0
35	4181547.0
36	9767420.0
37	1355244.0
38	3.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.42371304476172	18.464691961598227	16.270470801485256	31.8411241921548
2	24.117202682402503	25.193044314235642	33.72751285141317	16.96224015194869
3	19.617847155031352	29.27974166865363	30.51524518048944	20.587165995825572
4	23.188705209318133	34.17501802777901	22.377771285165363	20.25850547773749
5	25.033722404997967	35.24803209788372	22.55681564475808	17.161429852360232
6	18.652873306453255	39.04167465287016	22.99512917251406	19.31032286816253
7	20.067189002725197	20.25589765321083	38.65603586376476	21.020877480299212
8	21.312576542655698	22.948669265287105	28.62449362494699	27.11426056711021
9	23.39435020827977	22.961322811321075	28.903236484368954	24.7410904960302
10-14	23.022287032258788	28.99113302347071	25.82977390161354	22.156806042656964
15-19	22.849619197502328	28.145301514889	27.28392339198285	21.721155895625824
20-24	22.94424600299555	28.269597948010823	27.11826842431202	21.66788762468161
25-29	23.05572357159521	28.260577309331232	27.029231391192294	21.65446772788126
30-34	23.051936628951886	28.051001087450388	27.203649660790024	21.6934126228077
35-39	23.13869882657432	28.044678460418343	27.175708209891813	21.64091450311552
40-44	23.303265406759888	27.810001616519525	27.27046884165866	21.61626413506193
45-49	23.306952016567685	27.650894469301697	27.351830479299494	21.690323034831124
50-54	23.3852261124451	27.641772102451302	27.29332528006149	21.679676505042103
55-59	23.547787306494964	27.52955641568159	27.19200027230829	21.730656005515154
60-64	23.469100658336803	27.505626619810403	27.254519237598146	21.770753484254648
65-69	23.560742680232497	27.423558506335954	27.223221197293284	21.79247761613826
70-74	23.629818935301948	27.40316772320874	27.122791345720792	21.844221995768525
75-79	23.549494623092862	27.44116484597811	27.211587563554325	21.797752967374706
80-84	23.66176273472293	27.48589816635961	27.08168650898984	21.770652589927625
85-89	23.69794117764366	27.39950810209634	27.133693248503054	21.76885747175695
90-94	23.625022030727468	27.348407554133814	27.194669457412047	21.831900957726667
95-99	23.70504202764203	27.468087213778304	27.174024940006564	21.65284581857311
100-104	23.855379587516786	27.421727639386873	26.991812218723428	21.731080554372912
105-109	23.638192362492024	27.561749388077494	27.089109588698058	21.710948660732424
110-114	23.677560781072053	27.559269860542752	27.025743788661572	21.73742556972363
115-119	23.757401671437243	27.56341418708782	27.042379967827983	21.63680417364695
120-124	23.72770895650078	27.468321047328864	27.221697962426678	21.582272033743674
125-129	23.70627192334664	27.537067510333703	27.18342908844948	21.573231477870173
130-134	23.75138584729069	27.46456229738313	27.252534969206323	21.53151688611987
135-139	23.681181055120827	27.476030091724528	27.33647375108526	21.506315102069383
140-144	23.69269528449355	27.5996965304864	27.317073858358476	21.390534326661573
145-149	23.788966991682667	28.014641990631066	26.92188445125918	21.274506566427085
150	23.448720654770426	27.974494398173178	27.058281478463204	21.518503468593188
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	13.0
3	31.5
4	42.0
5	41.0
6	32.5
7	20.5
8	12.5
9	12.0
10	12.5
11	11.5
12	14.5
13	20.0
14	30.0
15	44.5
16	76.5
17	158.0
18	240.0
19	390.5
20	617.5
21	981.0
22	1458.5
23	2390.0
24	4039.5
25	6686.5
26	10665.5
27	17077.5
28	28586.0
29	43878.0
30	59432.5
31	78138.5
32	103648.5
33	136303.5
34	184085.0
35	254667.0
36	353815.5
37	479817.0
38	628003.0
39	811397.5
40	1022868.0
41	1222321.5
42	1408475.0
43	1581293.0
44	1699532.0
45	1750799.5
46	1748035.0
47	1688336.0
48	1579002.0
49	1431229.0
50	1241828.0
51	1039068.5
52	847193.0
53	672357.5
54	525836.5
55	399738.0
56	297057.5
57	220544.0
58	160912.0
59	115565.5
60	79483.0
61	52919.0
62	36006.0
63	25444.0
64	17735.5
65	12476.5
66	9315.0
67	7025.5
68	6215.0
69	4779.0
70	2734.5
71	1966.0
72	1520.0
73	1211.5
74	1043.0
75	647.5
76	221.0
77	68.5
78	21.5
79	2.5
80	2.0
81	1.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.145984939046351E-6
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.03452278739045116
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	1.6583939756185407E-6
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.06741288591190586
85-89	1.6583939756185407E-6
90-94	0.0
95-99	0.0
100-104	0.0
105-109	8.291969878092703E-7
110-114	0.0
115-119	0.0
120-124	0.0
125-129	8.291969878092703E-7
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.03667704116477964
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4119721E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.06846321103654
#Duplication Level	Percentage of total
1	29.786838419217393
2	10.612374662360155
3	7.019190804147716
4	5.001231416048675
5	3.88558171856921
6	3.0219400638560505
7	2.564255464411209
8	2.1371464137353464
9	1.8648954540917653
>10	20.86304111961969
>50	5.195386786852322
>100	6.095830409028105
>500	0.9878930011687233
>1k	0.8642463637190315
>5k	0.05659710788756511
>10k+	0.0435507952868943
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	4.145984939046352E-6	0.0	0.0	0.0013018392708605544	0.0
2	8.291969878092703E-6	2.0729924695231757E-5	0.0	4.145984939046352E-6	0.005082977535270827	0.0
3	3.731386445141716E-5	2.0729924695231757E-5	0.0	4.145984939046352E-6	0.006621137947657023	0.0
4	9.950363853711243E-5	2.0729924695231757E-5	0.0	8.291969878092703E-6	0.00794370714321281	0.0
5	1.0779560841520513E-4	2.0729924695231757E-5	0.0	8.291969878092703E-6	0.009461137630903773	0.0
6	1.285255331104369E-4	2.0729924695231757E-5	0.0	8.291969878092703E-6	0.011102947666766129	0.0
7	1.451094728666223E-4	2.4875909634278106E-5	0.0	8.291969878092703E-6	0.013383239383241622	0.0
8	2.4875909634278106E-4	2.4875909634278106E-5	0.0	1.2437954817139053E-5	0.0187481438943676	0.0
9	2.7363500597705917E-4	2.902189457332446E-5	0.0	1.2437954817139053E-5	0.02482615781500955	0.0
10-11	2.8399996832467506E-4	3.5240871981893986E-5	0.0	1.2437954817139053E-5	0.030904171735651506	0.0
12-13	3.1094887042847633E-4	4.975181926855621E-5	0.0	1.2437954817139053E-5	0.03669403970302973	0.0
14-15	3.4411674994084716E-4	5.182481173807939E-5	0.0	1.2437954817139053E-5	0.04439313373483881	0.0
16-17	3.731386445141716E-4	5.597079667712574E-5	0.0	1.2437954817139053E-5	0.04972072438151337	0.0
18-19	4.332554261303437E-4	5.804378914664892E-5	0.0	1.2437954817139053E-5	0.05684559949926452	0.0
20-21	4.975181926855621E-4	6.426276655521845E-5	0.0	1.2437954817139053E-5	0.06287386160063792	0.0
22-23	5.65926944179827E-4	8.291969878092703E-5	2.072992469523176E-6	1.2437954817139053E-5	0.06900577332548746	0.0
24-25	6.384816806131381E-4	9.121166865901973E-5	4.145984939046352E-6	1.2437954817139053E-5	0.07399339320716022	0.0
26-27	6.71649560125509E-4	9.743064606758926E-5	4.145984939046352E-6	1.2437954817139053E-5	0.07788232707998571	0.0
28-29	7.918831233578532E-4	1.0364962347615879E-4	4.145984939046352E-6	1.2437954817139053E-5	0.08206147989854443	0.0
30-31	9.867444154930316E-4	1.0364962347615879E-4	4.145984939046352E-6	1.2437954817139053E-5	0.08701385890823529	0.0
32-33	0.0011733137377501176	1.1401458582377466E-4	4.145984939046352E-6	1.2437954817139053E-5	0.09196209193298713	0.0
34-35	0.001343299120251018	1.2023356323234419E-4	4.145984939046352E-6	1.2437954817139053E-5	0.09514828135864423	0.0
36-37	0.0014531677211357462	1.2230655570186737E-4	1.2437954817139053E-5	1.2437954817139053E-5	0.09858530287311366	0.0
38-39	0.0015153574952214415	1.2437954817139053E-4	1.2437954817139053E-5	1.2437954817139053E-5	0.10198708351560119	0.0
40-41	0.0015568173446119048	1.3681750298852959E-4	1.865693222570858E-5	1.2437954817139053E-5	0.10486025107836032	0.0
42-43	0.001581693254246183	1.4718246533614547E-4	2.0729924695231757E-5	1.2437954817139053E-5	0.10778524345285752	0.0
44-45	0.0016438830283318781	1.5754742768376136E-4	2.0729924695231757E-5	1.2437954817139053E-5	0.11025003149912058	0.0
46-47	0.0016936348476004346	2.0937223942184074E-4	2.0729924695231757E-5	1.2437954817139053E-5	0.1128557830333112	0.0
48-49	0.0017475326518080372	2.1559121683041027E-4	2.0729924695231757E-5	1.2437954817139053E-5	0.1152791112301838	0.0
50-51	0.0018117954183632557	2.1559121683041027E-4	2.0729924695231757E-5	1.2437954817139053E-5	0.11745160733824409	0.0
52-53	0.001876058184918474	2.2388318670850296E-4	2.0729924695231757E-5	1.2437954817139053E-5	0.11965519833334723	0.0
54-55	0.001969342846047017	2.4046712646468837E-4	2.0729924695231757E-5	1.2437954817139053E-5	0.12170124190076659	0.0
56-57	0.0020190946653155734	2.4875909634278106E-4	2.0729924695231757E-5	1.2437954817139053E-5	0.12387581100129641	0.0
58-59	0.002083357431870792	2.653430360989665E-4	2.0729924695231757E-5	1.6583939756185407E-5	0.12573528524645872	0.0
60-61	0.0021890800478164734	2.6948902103801284E-4	2.0729924695231757E-5	1.6583939756185407E-5	0.12729417558354011	0.0
62-63	0.002253342814371692	2.7363500597705917E-4	2.0729924695231757E-5	1.6583939756185407E-5	0.12936924104553282	0.0
64-65	0.0022844377014145396	2.819269758551519E-4	2.0729924695231757E-5	2.0729924695231757E-5	0.1310483649458466	0.0
66-67	0.0023569924378478505	3.0265690055038366E-4	2.0729924695231757E-5	2.4875909634278106E-5	0.13229008743509096	0.0
68-69	0.002371503385134513	3.130218628979995E-4	2.2802917164754933E-5	2.4875909634278106E-5	0.1335276639393963	4.145984939046352E-6
70-71	0.0023984522872383144	3.275328101846617E-4	2.4875909634278106E-5	2.4875909634278106E-5	0.1347714594211102	4.145984939046352E-6
72-73	0.0024419851290983013	3.4411674994084716E-4	2.902189457332446E-5	2.6948902103801283E-5	0.1356379702733709	4.145984939046352E-6
74-75	0.002475153008610672	3.461897424103703E-4	3.316787951237081E-5	2.902189457332446E-5	0.13677811613160865	6.2189774085695275E-6
76-77	0.0025103938805925655	3.482627348798935E-4	3.731386445141716E-5	2.902189457332446E-5	0.13826652472472628	8.291969878092703E-6
78-79	0.0025808756245563535	3.6070068969703254E-4	3.731386445141716E-5	2.902189457332446E-5	0.139985035481961	1.0364962347615878E-5
80-81	0.002599532556782062	3.752116369836948E-4	3.731386445141716E-5	2.902189457332446E-5	0.14155429078139004	1.6583939756185407E-5
82-83	0.0026202624814772937	3.980145541484497E-4	4.3532841859986686E-5	2.902189457332446E-5	0.14334121029011904	1.6583939756185407E-5
84-85	0.0026327004362944333	4.063065240265424E-4	4.5605834329509866E-5	2.902189457332446E-5	0.1448503488079319	1.6583939756185407E-5
86-87	0.0026637953233372806	4.166714863741583E-4	4.5605834329509866E-5	2.902189457332446E-5	0.14662068437690468	1.6583939756185407E-5
88-89	0.0026783062706239426	4.228904637827278E-4	4.975181926855621E-5	2.902189457332446E-5	0.14842211483292034	1.6583939756185407E-5
90-91	0.002701109187788698	4.477663734170059E-4	4.975181926855621E-5	2.902189457332446E-5	0.14941507822582195	1.865693222570858E-5
92-93	0.0027197661200144066	4.5191235835605225E-4	5.3897804207602566E-5	3.731386445141716E-5	0.15039974964884545	2.0729924695231757E-5
94-95	0.0027301310823620224	4.5605834329509864E-4	5.3897804207602566E-5	3.938685692094034E-5	0.15145697580830225	2.0729924695231757E-5
96-97	0.0027342770673010686	4.6227732070366814E-4	5.597079667712574E-5	4.5605834329509866E-5	0.1523898224195877	2.4875909634278106E-5
98-99	0.0027550069919963006	4.767882679903304E-4	5.804378914664892E-5	4.5605834329509866E-5	0.15323353035468362	2.902189457332446E-5
100-101	0.0027674449468134393	4.767882679903304E-4	5.804378914664892E-5	4.5605834329509866E-5	0.1540440704102672	2.902189457332446E-5
102-103	0.0027695179392829626	4.8093425292937674E-4	6.426276655521845E-5	4.5605834329509866E-5	0.1549333841796926	2.902189457332446E-5
104-105	0.002784028886569625	4.912992152769926E-4	6.633575902474163E-5	4.5605834329509866E-5	0.15575843518256283	2.902189457332446E-5
106-107	0.0028151237736124724	5.161751249112708E-4	6.840875149426479E-5	4.5605834329509866E-5	0.15649642050171308	2.902189457332446E-5
108-109	0.0028151237736124724	5.30686072197933E-4	7.048174396378797E-5	4.5605834329509866E-5	0.15724477078321097	2.902189457332446E-5
110-111	0.002827561728429612	5.514159968931648E-4	7.048174396378797E-5	4.5605834329509866E-5	0.15809884368065452	2.902189457332446E-5
112-113	0.002844145668185797	5.597079667712574E-4	7.048174396378797E-5	4.5605834329509866E-5	0.15890523775129906	2.902189457332446E-5
114-115	0.0028482916531248435	5.679999366493501E-4	7.048174396378797E-5	4.5605834329509866E-5	0.1598235734152978	2.902189457332446E-5
116-117	0.0028503646455943664	5.679999366493501E-4	7.462772890283432E-5	4.5605834329509866E-5	0.16078129593621748	2.902189457332446E-5
118-119	0.0028607296079419826	5.721459215883964E-4	7.462772890283432E-5	4.5605834329509866E-5	0.16157525205204487	2.902189457332446E-5
120-121	0.002864875592881029	5.742189140579197E-4	7.462772890283432E-5	4.5605834329509866E-5	0.16248322275369603	2.902189457332446E-5
122-123	0.002864875592881029	5.78364898996966E-4	7.462772890283432E-5	4.5605834329509866E-5	0.1631445073514739	3.316787951237081E-5
124-125	0.0028773135476981675	5.90802853814105E-4	8.49926912504502E-5	4.5605834329509866E-5	0.16390736858025845	3.731386445141716E-5
126-127	0.0028980434723933995	5.928758462836282E-4	8.706568371997337E-5	4.5605834329509866E-5	0.16484436117648293	4.145984939046351E-5
128-129	0.0029187733970886314	5.990948236921977E-4	8.913867618949655E-5	4.5605834329509866E-5	0.16587463843383593	4.3532841859986686E-5
130-131	0.0029312113519057706	6.032408086312441E-4	9.121166865901973E-5	4.5605834329509866E-5	0.16692149963094516	4.767882679903304E-5
132-133	0.002960233246479095	6.094597860398137E-4	9.121166865901973E-5	4.5605834329509866E-5	0.16776106158110204	5.182481173807939E-5
134-135	0.0029851091561133728	6.094597860398137E-4	9.121166865901973E-5	4.5605834329509866E-5	0.168517703832478	5.804378914664892E-5
136-137	0.0029934011259914657	6.094597860398137E-4	9.32846611285429E-5	4.5605834329509866E-5	0.16939872563202535	6.218977408569527E-5
138-139	0.0030058390808086048	6.156787634483832E-4	9.535765359806608E-5	4.767882679903304E-5	0.17015951386834033	6.426276655521845E-5
>>END_MODULE
