##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.4d.R.3.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24236895
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.541407882486595	36.0	35.0	38.0	32.0	39.0
2	35.197263675895776	35.0	34.0	37.0	32.0	39.0
3	35.33377043552815	35.0	34.0	37.0	33.0	39.0
4	35.396398507317045	36.0	35.0	37.0	33.0	39.0
5	35.31298501726397	35.0	34.0	37.0	33.0	39.0
6	35.276650948894236	35.0	34.0	37.0	33.0	39.0
7	35.30814186388149	35.0	34.0	37.0	33.0	39.0
8	35.44719181231754	36.0	34.0	38.0	33.0	39.0
9	35.41520673337075	36.0	34.0	38.0	33.0	39.0
10-14	35.3800408674461	35.4	34.2	37.6	33.0	39.0
15-19	35.35306123164704	35.8	34.0	38.0	33.0	39.0
20-24	35.31968034684311	35.6	34.0	37.6	32.8	39.0
25-29	35.30881681007406	35.8	34.0	37.8	32.0	39.0
30-34	35.288200184058226	35.4	34.0	37.4	32.0	39.0
35-39	35.26252247245367	35.4	34.0	37.2	32.0	39.0
40-44	35.24131887356033	35.6	34.0	37.0	32.0	39.0
45-49	35.21168235452602	35.0	34.0	37.0	32.0	39.0
50-54	35.18770668437521	35.0	34.0	37.0	32.0	39.0
55-59	35.156807957454944	35.0	34.0	37.0	32.0	39.0
60-64	35.11622741279359	35.0	34.0	37.0	32.0	39.0
65-69	35.07972582296536	35.0	34.0	37.0	32.0	39.0
70-74	35.05749325563361	35.0	34.0	37.0	32.0	39.0
75-79	35.0065623092397	35.0	34.0	37.0	32.0	39.0
80-84	34.935596263465264	35.0	34.0	37.0	31.0	39.0
85-89	34.914212996342975	35.0	34.0	37.0	31.0	39.0
90-94	34.86245551668232	35.0	34.0	37.0	31.0	39.0
95-99	34.79845816058534	35.0	34.0	37.0	31.0	39.0
100-104	34.7334082604228	35.0	34.0	37.0	31.0	39.0
105-109	34.67417911411508	35.0	34.0	37.0	30.4	39.0
110-114	34.61083282326386	35.0	34.0	37.0	30.0	39.0
115-119	34.55070065699422	35.0	34.0	37.0	30.0	39.0
120-124	34.476455362784705	35.0	34.0	37.0	30.0	39.0
125-129	34.39578943589928	35.0	34.0	37.0	30.0	39.0
130-134	34.32122099798674	35.0	34.0	37.0	29.6	39.0
135-139	34.23295004578763	35.0	34.0	37.0	29.0	39.0
140-144	34.13236409201757	35.0	34.0	37.0	29.0	39.0
145-149	34.02909907395316	35.0	34.0	37.0	28.6	39.0
150	33.95689447843876	35.0	34.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	17.0
21	330.0
22	2705.0
23	12165.0
24	38447.0
25	89569.0
26	174015.0
27	295043.0
28	427031.0
29	555615.0
30	703434.0
31	902219.0
32	1191184.0
33	1637823.0
34	2429582.0
35	4097034.0
36	1.014155E7
37	1539121.0
38	10.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.433677599118106	18.9729756626406	16.188295414420676	31.405051323820622
2	24.396876101368434	25.599493070357944	32.94040482249912	17.063226005774503
3	19.739050732364852	29.553934198254357	30.287142804389756	20.41987226499104
4	23.370452361987788	33.95539321352838	22.543877835836646	20.130276588647185
5	25.071235403709924	35.24659408723766	22.525269841702084	17.156900667350335
6	18.716172182946703	39.22568876912657	22.733873295238517	19.324265752688206
7	20.155697343067143	20.686681222437166	38.264931966942626	20.892689467553062
8	21.392064511236462	23.366801868259195	28.36752101981384	26.8736126006905
9	23.413085710855288	23.166296672903027	28.766770661010828	24.653846955230858
10-14	22.955310265240744	29.29638577298277	25.679356416565373	22.068947545211113
15-19	22.81226782556099	28.29896981440898	27.19875710151816	21.690005258511867
20-24	22.927550396674516	28.380453431091844	27.079206014580326	21.61279015765331
25-29	23.045878607800216	28.334665805995364	26.999219165656328	21.620236420548096
30-34	23.038924746754898	28.125716598598956	27.17261101308563	21.66274764156052
35-39	23.132827864295326	28.09380326976702	27.143720348666772	21.62964851727088
40-44	23.289340486889927	27.87284922429214	27.24660481468439	21.59120547413355
45-49	23.31049996296968	27.679740329774088	27.335251483327383	21.67450822392885
50-54	23.38406651003824	27.64899734598015	27.295881153884448	21.671054990097165
55-59	23.54326822804654	27.535749113077397	27.19957651341065	21.721406145465416
60-64	23.461795745700925	27.50333984613128	27.261161134708058	21.77370327345974
65-69	23.559060680008724	27.42618392331196	27.214325927475446	21.800429469203873
70-74	23.626107222067844	27.36246288973897	27.135073201414624	21.876356686778568
75-79	23.53266868548962	27.425407421206387	27.217955105222842	21.823968788081146
80-84	23.653073235099615	27.468722507303077	27.08059264667022	21.79761161092708
85-89	23.687875426086972	27.37674817466096	27.153802121167786	21.78157427808429
90-94	23.62177168321272	27.315010441725313	27.202955659130428	21.860262215931535
95-99	23.70433423918369	27.448998726940886	27.179047481123302	21.667619552752114
100-104	23.861033354313744	27.395717149412086	26.99501070578554	21.74823879048863
105-109	23.62613940440803	27.544520863749256	27.096811699683478	21.732528032159234
110-114	23.66319055030102	27.55288602399677	27.013979530084026	21.76994389561818
115-119	23.738379854350157	27.54825153964648	27.031724154434798	21.68164445156857
120-124	23.71506993779525	27.454477976655014	27.216526704431406	21.61392538111833
125-129	23.707770322889957	27.526508655502287	27.168764810839015	21.59695621076875
130-134	23.741013855116343	27.433340780656927	27.258164876317696	21.567480487909034
135-139	23.662141540820308	27.440589233893203	27.349241724239015	21.54802750104747
140-144	23.680782737894297	27.555505171396792	27.327325776072602	21.436386314636312
145-149	23.783407317378316	27.990537033064133	26.91031057302105	21.3157450765365
150	23.41102274033039	27.968483586697058	27.03789408668066	21.582599586291888
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.5
2	10.0
3	27.5
4	38.5
5	32.0
6	23.0
7	19.0
8	9.5
9	4.0
10	7.0
11	14.0
12	16.5
13	16.0
14	25.0
15	36.5
16	88.0
17	167.5
18	268.5
19	486.5
20	733.5
21	1087.5
22	1715.0
23	2861.5
24	4663.0
25	7555.0
26	11957.5
27	19098.5
28	31982.0
29	49320.0
30	65486.0
31	84147.5
32	110085.0
33	143950.5
34	193353.5
35	265270.5
36	365592.0
37	490139.5
38	636415.0
39	820042.0
40	1030924.0
41	1225163.0
42	1403916.0
43	1570766.5
44	1686067.5
45	1737394.5
46	1735847.5
47	1679990.5
48	1575382.5
49	1430143.5
50	1245936.5
51	1047100.5
52	855833.0
53	682675.5
54	533951.0
55	406004.0
56	303609.0
57	225609.5
58	164663.0
59	118559.0
60	81746.5
61	54310.0
62	36935.0
63	26356.5
64	18574.5
65	13299.5
66	9988.0
67	7448.5
68	6705.5
69	5182.5
70	2951.5
71	2200.0
72	1661.5
73	1245.0
74	1048.5
75	638.5
76	210.0
77	77.0
78	25.5
79	5.0
80	1.5
81	1.5
82	1.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.125941049792063E-6
2	4.125941049792063E-6
3	0.0
4	0.0
5	0.0
6	0.0
7	4.125941049792063E-6
8	4.125941049792063E-6
9	0.0
10-14	8.251882099584125E-7
15-19	0.0
20-24	0.027132188343432607
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.251882099584125E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.052542208892682
85-89	1.650376419916825E-6
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	8.251882099584125E-7
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	8.251882099584125E-7
145-149	0.029010316709297955
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4236895E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.9805346113908
#Duplication Level	Percentage of total
1	28.73407371060528
2	10.52656691798516
3	6.9019419328266896
4	4.940416399457166
5	3.978811454468808
6	3.1756708884054916
7	2.567372397048303
8	2.1466266482568845
9	1.8169491895296184
>10	21.301824181308255
>50	5.334125232499859
>100	6.433661602914474
>500	1.0715578289627752
>1k	0.9095419935628521
>5k	0.10285596205713368
>10k+	0.058003660111230176
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	4.125941049792063E-6	4.125941049792063E-6	4.125941049792063E-6	0.0011305078476430252	0.0
2	1.2377823149376188E-5	1.2377823149376188E-5	4.125941049792063E-6	8.251882099584126E-6	0.005862962231754521	0.0
3	4.951129259750475E-5	1.6503764199168252E-5	4.125941049792063E-6	8.251882099584126E-6	0.007595857472667188	0.0
4	9.90225851950095E-5	1.6503764199168252E-5	4.125941049792063E-6	8.251882099584126E-6	0.00901930713484545	0.0
5	1.0314852624480157E-4	1.6503764199168252E-5	8.251882099584126E-6	1.2377823149376188E-5	0.010822343373604581	0.0
6	1.114004083443857E-4	1.6503764199168252E-5	8.251882099584126E-6	1.2377823149376188E-5	0.012703772492309761	0.0
7	1.1965229044396982E-4	3.3007528398336504E-5	8.251882099584126E-6	1.2377823149376188E-5	0.015076188595940197	0.0
8	1.7328952409126664E-4	3.713346944812857E-5	8.251882099584126E-6	1.2377823149376188E-5	0.02148377504626727	0.0
9	1.774154651410587E-4	4.125941049792063E-5	8.251882099584126E-6	1.2377823149376188E-5	0.02838647442256939	0.0
10-11	2.0629705248960314E-4	4.538535154771269E-5	8.251882099584126E-6	1.4440793674272219E-5	0.03563987878810384	2.0629705248960315E-6
12-13	2.805639913858603E-4	4.951129259750476E-5	8.251882099584126E-6	1.6503764199168252E-5	0.042119669206802275	4.125941049792063E-6
14-15	3.424531071327412E-4	5.363723364729682E-5	8.251882099584126E-6	1.6503764199168252E-5	0.051642341149722354	4.125941049792063E-6
16-17	3.816495471057658E-4	5.776317469708888E-5	8.251882099584126E-6	1.856673472406428E-5	0.05813863533261995	4.125941049792063E-6
18-19	4.2497192812858246E-4	6.601505679667301E-5	8.251882099584126E-6	2.0629705248960314E-5	0.06689800818132852	4.125941049792063E-6
20-21	4.7654619125098326E-4	7.426693889625712E-5	8.251882099584126E-6	2.4755646298752376E-5	0.07369137011981114	4.125941049792063E-6
22-23	5.363723364729682E-4	1.0314852624480157E-4	8.251882099584126E-6	2.4755646298752376E-5	0.08130579432720239	4.125941049792063E-6
24-25	5.961984816949531E-4	1.1965229044396983E-4	1.2377823149376188E-5	2.888158734854444E-5	0.08717288250000671	4.125941049792063E-6
26-27	6.353949216679777E-4	1.3615605464313807E-4	1.2377823149376188E-5	3.3007528398336504E-5	0.09141228692866804	4.125941049792063E-6
28-29	7.509212710621555E-4	1.3615605464313807E-4	1.2377823149376188E-5	3.3007528398336504E-5	0.09619425260537705	4.125941049792063E-6
30-31	9.469034709272784E-4	1.4853387779251425E-4	1.2377823149376188E-5	3.3007528398336504E-5	0.10189630313618968	4.125941049792063E-6
32-33	0.0010768706139957286	1.691635830414746E-4	1.4440793674272219E-5	3.3007528398336504E-5	0.10794286974465994	4.125941049792063E-6
34-35	0.0011738302286658417	1.8773031776553886E-4	1.6503764199168252E-5	3.3007528398336504E-5	0.11161495727897489	4.125941049792063E-6
36-37	0.0012254044917882428	2.0010814091491505E-4	1.6503764199168252E-5	3.3007528398336504E-5	0.115660442478296	4.125941049792063E-6
38-39	0.0012852306370102276	2.021711114398111E-4	1.6503764199168252E-5	3.713346944812857E-5	0.11955326785877482	4.125941049792063E-6
40-41	0.0013264900475081483	2.1248596406429123E-4	1.6503764199168252E-5	3.713346944812857E-5	0.12282720208178483	4.125941049792063E-6
42-43	0.0013368049001326284	2.2692675773856345E-4	1.856673472406428E-5	3.713346944812857E-5	0.12593403569227823	4.125941049792063E-6
44-45	0.0013533086643317967	2.5168240403731585E-4	2.0629705248960314E-5	3.713346944812857E-5	0.1287066680777385	4.125941049792063E-6
46-47	0.0014090088685039894	2.805639913858603E-4	2.0629705248960314E-5	3.713346944812857E-5	0.13176192742510953	4.125941049792063E-6
48-49	0.0014605831316263902	2.888158734854444E-4	2.0629705248960314E-5	3.713346944812857E-5	0.13439634078540175	4.125941049792063E-6
50-51	0.0015286611589479593	2.929418145352365E-4	2.0629705248960314E-5	3.713346944812857E-5	0.1369276056194492	4.125941049792063E-6
52-53	0.0015802354220703601	2.9500478506013254E-4	2.0629705248960314E-5	3.713346944812857E-5	0.139531074421868	4.125941049792063E-6
54-55	0.0016276837441429688	3.011936966348206E-4	2.0629705248960314E-5	3.91964399730246E-5	0.1415445336541665	4.125941049792063E-6
56-57	0.0016936988009396419	3.011936966348206E-4	2.0629705248960314E-5	4.125941049792063E-5	0.14383236796627621	4.125941049792063E-6
58-59	0.001778280592460379	3.0531963768461266E-4	2.0629705248960314E-5	4.538535154771269E-5	0.1461697135709834	4.125941049792063E-6
60-61	0.001836043767157468	3.135715197841968E-4	2.0629705248960314E-5	4.538535154771269E-5	0.14785309751929857	4.125941049792063E-6
62-63	0.0018834920892300767	3.218234018837809E-4	2.0629705248960314E-5	4.538535154771269E-5	0.14994288666101824	4.125941049792063E-6
64-65	0.001899995853429245	3.383271660829492E-4	2.0629705248960314E-5	4.538535154771269E-5	0.15183256766182301	4.125941049792063E-6
66-67	0.001972199821800606	3.5895687133190947E-4	2.0629705248960314E-5	4.538535154771269E-5	0.15323538761875233	6.188911574688095E-6
68-69	0.0020010814091491504	3.6927172395638964E-4	2.0629705248960314E-5	4.538535154771269E-5	0.15473929313140153	8.251882099584126E-6
70-71	0.0020196481438732147	3.816495471057658E-4	2.0629705248960314E-5	4.538535154771269E-5	0.15617099467567935	8.251882099584126E-6
72-73	0.0020485297312217592	3.96090340780038E-4	2.0629705248960314E-5	4.744832207260872E-5	0.1571364648813307	8.251882099584126E-6
74-75	0.0020609075543711356	4.064051934045182E-4	2.0629705248960314E-5	4.951129259750475E-5	0.15828141352264802	8.251882099584126E-6
76-77	0.0020856632006698876	4.270348986534785E-4	2.0629705248960314E-5	4.951129259750475E-5	0.1597419966542744	8.251882099584126E-6
78-79	0.0021475523164167687	4.456016333775428E-4	2.4755646298752376E-5	4.951129259750475E-5	0.16144394733731365	8.251882099584126E-6
80-81	0.002166119051140833	4.5385351547712696E-4	2.888158734854444E-5	4.951129259750475E-5	0.16294166393838816	8.251882099584126E-6
82-83	0.002176433903765313	4.7242025020119123E-4	3.094455787344047E-5	4.951129259750475E-5	0.1647818336465954	8.251882099584126E-6
84-85	0.0021991265795391693	4.7448322072608725E-4	3.3007528398336504E-5	4.951129259750475E-5	0.16671071108737318	8.251882099584126E-6
86-87	0.002217693314263234	4.7654619125098326E-4	3.3007528398336504E-5	4.951129259750475E-5	0.1687922483469933	8.251882099584126E-6
88-89	0.0022445119310868823	4.806721323007753E-4	3.713346944812856E-5	4.951129259750475E-5	0.17074794440459473	8.251882099584126E-6
90-91	0.002267204606860739	5.095537196493198E-4	4.125941049792063E-5	4.951129259750475E-5	0.17181862610701576	1.2377823149376188E-5
92-93	0.0022692675773856347	5.116166901742158E-4	4.125941049792063E-5	5.363723364729682E-5	0.17288930780943682	1.2377823149376188E-5
94-95	0.0022816454005350107	5.23994513323592E-4	4.125941049792063E-5	5.363723364729682E-5	0.17391873010135994	1.2377823149376188E-5
96-97	0.0022919602531594908	5.322463954231761E-4	4.125941049792063E-5	5.363723364729682E-5	0.1748718224838619	1.2377823149376188E-5
98-99	0.0023022751057839713	5.384353069978643E-4	4.538535154771269E-5	5.363723364729682E-5	0.17587442615896137	1.2377823149376188E-5
100-101	0.002310526987883555	5.446242185725523E-4	4.744832207260872E-5	5.363723364729682E-5	0.17676150348466665	1.6503764199168252E-5
102-103	0.0023146529289333473	5.446242185725523E-4	4.951129259750475E-5	5.363723364729682E-5	0.17771046992611883	1.6503764199168252E-5
104-105	0.0023208418405080353	5.487501596223444E-4	4.951129259750475E-5	5.363723364729682E-5	0.17863468072127225	1.6503764199168252E-5
106-107	0.002343534516281892	5.776317469708888E-4	4.951129259750475E-5	5.363723364729682E-5	0.17936290931656057	1.6503764199168252E-5
108-109	0.002347660457331684	5.87946599595369E-4	5.776317469708888E-5	5.363723364729682E-5	0.18019016049704387	2.2692675773856347E-5
110-111	0.0023744790741553326	6.044503637945372E-4	6.188911574688094E-5	5.363723364729682E-5	0.1811556307026952	2.4755646298752376E-5
112-113	0.0023889198678296044	6.147652164190173E-4	6.188911574688094E-5	5.363723364729682E-5	0.18206540070417437	2.4755646298752376E-5
114-115	0.002401297690978981	6.188911574688094E-4	6.188911574688094E-5	5.363723364729682E-5	0.18298548555827798	2.4755646298752376E-5
116-117	0.0024054236320287725	6.353949216679777E-4	6.601505679667301E-5	5.363723364729682E-5	0.1840190337912509	2.681861682364841E-5
118-119	0.0024095495730785646	6.415838332426658E-4	6.601505679667301E-5	5.363723364729682E-5	0.1850154485547757	3.3007528398336504E-5
120-121	0.0024095495730785646	6.457097742924578E-4	7.426693889625714E-5	5.570020417219285E-5	0.18602217817092492	3.91964399730246E-5
122-123	0.0024095495730785646	6.56024626916938E-4	7.426693889625714E-5	5.776317469708888E-5	0.1868865628208564	4.125941049792063E-5
124-125	0.0024136755141283567	6.622135384916261E-4	7.839287994604919E-5	5.776317469708888E-5	0.1878644108496571	4.125941049792063E-5
126-127	0.002417801455178149	6.725283911161062E-4	8.251882099584126E-5	5.776317469708888E-5	0.18898047790362585	4.744832207260872E-5
128-129	0.0024301792783275253	6.807802732156904E-4	8.251882099584126E-5	5.776317469708888E-5	0.18999133346082492	5.9826145221984916E-5
130-131	0.0024363681899022133	6.952210668899626E-4	8.251882099584126E-5	5.776317469708888E-5	0.19104757436957168	7.632990942115316E-5
132-133	0.0024569978951511735	7.055359195144428E-4	8.251882099584126E-5	5.9826145221984916E-5	0.19185625881533092	8.251882099584126E-5
134-135	0.0024817535414499254	7.199767131887149E-4	8.251882099584126E-5	6.188911574688094E-5	0.1927020767305383	8.870773257052935E-5
136-137	0.002485879482499718	7.302915658131951E-4	8.251882099584126E-5	6.395208627177698E-5	0.1935375797931212	9.283367362032143E-5
138-139	0.0025085721582735745	7.364804773878832E-4	8.251882099584126E-5	6.601505679667301E-5	0.19426993432945927	9.90225851950095E-5
>>END_MODULE
