##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.4d.S.2.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24097071
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.92443185314929	36.0	34.0	38.0	30.0	39.0
2	34.792097512598104	35.0	34.0	38.0	31.0	39.0
3	34.93590768770196	35.0	34.0	38.0	31.0	39.0
4	34.95109102678911	36.0	34.0	38.0	31.0	39.0
5	34.81744059267618	35.0	34.0	37.0	31.0	39.0
6	34.80431443306948	35.0	34.0	37.0	32.0	39.0
7	34.84085746354816	35.0	34.0	37.0	32.0	39.0
8	34.972398927653906	35.0	34.0	37.0	32.0	39.0
9	34.93438675596715	35.0	34.0	37.0	32.0	39.0
10-14	34.91915944473085	35.2	34.0	37.4	31.6	39.0
15-19	34.93384749540722	35.0	34.0	37.0	31.6	39.0
20-24	34.92131620477858	35.0	34.0	37.0	31.2	39.0
25-29	34.92311623267409	35.0	34.0	37.0	31.4	39.0
30-34	34.90324309539529	35.0	34.0	37.0	31.0	39.0
35-39	34.89412538146233	35.0	34.0	37.0	31.0	39.0
40-44	34.851304351470766	35.0	34.0	37.0	31.0	39.0
45-49	34.74910695992886	35.0	34.0	37.0	31.0	39.0
50-54	34.72578121216475	35.0	34.0	37.0	31.0	39.0
55-59	34.717140278169076	35.0	34.0	37.0	31.0	39.0
60-64	34.689194566426764	35.0	34.0	37.0	31.0	39.0
65-69	34.65471147095014	35.0	34.0	37.0	31.0	39.0
70-74	34.658091856889996	35.0	34.0	37.0	31.0	39.0
75-79	34.64282685642583	35.0	34.0	37.0	31.0	39.0
80-84	34.62356967782516	35.0	34.0	37.0	31.0	39.0
85-89	34.61110764872627	35.0	34.0	37.0	30.4	39.0
90-94	34.585114655635955	35.0	34.0	37.0	30.0	39.0
95-99	34.56684947311646	35.0	34.0	37.0	30.0	39.0
100-104	34.52850560136541	35.0	34.0	37.0	30.0	39.0
105-109	34.499440815856836	35.0	34.0	37.0	30.0	39.0
110-114	34.46852739073558	35.0	34.0	37.0	30.0	39.0
115-119	34.4327800503223	35.0	34.0	37.0	30.0	39.0
120-124	34.3910966938679	35.0	34.0	37.0	30.0	39.0
125-129	34.37247988355099	35.0	34.0	37.0	30.0	39.0
130-134	34.32674847494951	35.0	34.0	37.0	30.0	39.0
135-139	34.28421626844192	35.0	34.0	37.0	30.0	39.0
140-144	34.24067045326795	35.0	34.0	37.0	29.4	39.0
145-149	34.20458330392104	35.0	34.0	37.0	29.0	39.0
150	34.19995882487129	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	15.0
22	311.0
23	3209.0
24	20481.0
25	77945.0
26	196924.0
27	359335.0
28	534918.0
29	700354.0
30	880350.0
31	1108522.0
32	1417597.0
33	1873036.0
34	2588188.0
35	4048488.0
36	8724853.0
37	1562536.0
38	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.849926592625465	11.032332858890435	14.356376805543785	38.76136374294031
2	23.295011356575902	12.775127015537006	36.18160843907379	27.748253188813305
3	21.261974988454526	17.392259238447945	28.28833649518569	33.05742927791184
4	26.607632602964483	25.062053244032217	21.81499576213651	26.5153183908668
5	26.794649653690072	30.60990808960613	23.735500148420613	18.85994210828318
6	19.721523739220835	36.62868375287063	24.999428353571552	18.65036415433698
7	16.702870943473147	24.33088001893675	39.92866598959931	19.037583047990793
8	19.079001671864606	23.892948376620588	31.10002648872287	25.928023462791934
9	20.150157786935928	22.482069590919256	32.30310911060822	25.0646635115366
10-14	21.033651561720053	28.707887575139335	26.507943724790156	23.750517138350457
15-19	21.282941778883718	27.59608452937195	27.20583937103357	23.915134320710763
20-24	21.230146700400674	27.697003510322332	27.09794278284407	23.97490700643293
25-29	21.186490272690804	27.876928515962895	26.807534580198222	24.129046631148075
30-34	21.17916171947756	27.663536113204383	27.012621520746738	24.144680646571317
35-39	21.45261231648266	27.44901884637957	26.91598744775396	24.18238138938381
40-44	21.606740688034023	27.534907033678792	26.768571166061044	24.08978111222614
45-49	21.809876229970094	27.459669820706946	26.68428128629467	24.046172663028294
50-54	21.81103056836849	27.64312536126315	26.627756946778696	23.91808712358966
55-59	21.724617555045068	27.602141979583845	26.689758413611393	23.98348205175969
60-64	21.917845750081224	27.442696792970857	26.484115868685432	24.15534158826249
65-69	21.798643704519062	27.538776154938187	26.65787838486296	24.004701755679786
70-74	22.138441032390833	27.319353540892088	26.594315617960074	23.947889808757004
75-79	21.996433470282117	27.194313106722408	26.604944390383857	24.20430903261162
80-84	22.24955023536493	27.201308641622823	26.597087742863774	23.95205338014847
85-89	22.117719524615325	27.204101594280665	26.605101286799037	24.07307759430497
90-94	22.09605087881924	26.980492024969777	26.59688552914713	24.326571567063855
95-99	22.319752277069128	26.983900677039703	26.771070481131826	23.92527656475934
100-104	22.28053492175414	26.82734991248101	26.80680134507712	24.08531382068773
105-109	22.35925644397647	26.753460540292608	26.74371079768515	24.14357221804578
110-114	22.318867307370052	26.79725881889814	26.748013803178793	24.13586007055301
115-119	22.431128112724124	26.959264740179922	26.645955149715363	23.963651997380595
120-124	22.34718535244159	26.817372390392723	26.677462291704856	24.157979965460825
125-129	22.69405684712058	26.78156640005555	26.576594376224875	23.947782376598997
130-134	22.451952500795887	27.004227229274598	26.49180788465129	24.052012385278225
135-139	22.55025925940466	26.828159160414167	26.538044957198142	24.08353662298303
140-144	22.539376383623264	26.688843924432597	26.770113596439867	24.001666095504273
145-149	22.38772867692766	27.194050054121142	26.63695205083337	23.781269218117824
150	22.08481347690669	26.726744024152673	26.80508143675554	24.3833610621851
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.0
9	1.0
10	3.0
11	8.0
12	14.0
13	18.0
14	24.5
15	55.5
16	106.0
17	217.0
18	521.0
19	943.5
20	1533.0
21	2514.5
22	4182.0
23	6833.5
24	11107.0
25	17122.5
26	24745.5
27	36084.5
28	55551.0
29	82056.0
30	110976.0
31	140187.5
32	170739.0
33	205524.5
34	240764.5
35	286504.0
36	354493.5
37	425591.5
38	498005.0
39	602541.5
40	743717.0
41	903328.0
42	1068685.0
43	1229546.0
44	1370541.0
45	1476534.5
46	1548963.5
47	1566943.0
48	1518376.5
49	1463614.0
50	1377713.5
51	1227320.0
52	1038998.0
53	869181.0
54	725508.5
55	597431.5
56	502506.0
57	410767.0
58	336176.0
59	273312.0
60	207210.0
61	143911.0
62	85996.0
63	49578.0
64	31765.0
65	19904.0
66	13365.0
67	6710.0
68	3079.0
69	1888.0
70	1466.5
71	1350.0
72	1216.5
73	843.0
74	435.5
75	144.5
76	37.0
77	22.0
78	10.5
79	3.5
80	3.5
81	1.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.5354563216417465E-4
2	7.884775705727887E-5
3	0.15712283040540487
4	9.544728485881127E-5
5	5.809834730536338E-5
6	3.319905560306479E-5
7	4.149881950383098E-5
8	9.959716680919435E-5
9	7.884775705727887E-5
10-14	1.5686553772448114E-4
15-19	2.689123503848248E-4
20-24	3.386303671512608E-4
25-29	2.390332003420665E-4
30-34	2.2409362532068732E-4
35-39	1.2034657656110985E-4
40-44	2.697423267749014E-4
45-49	1.7429504191609016E-4
50-54	1.012571195893476E-4
55-59	1.435859154832552E-4
60-64	1.4109598631302536E-4
65-69	0.06226067890159763
70-74	1.7346506552601352E-4
75-79	1.3943603353287211E-4
80-84	9.544728485881127E-5
85-89	8.963745012827493E-5
90-94	1.4026600992294872E-4
95-99	8.46575917878152E-5
100-104	5.809834730536338E-5
105-109	1.0208709597942422E-4
110-114	5.3948465354980285E-5
115-119	4.5648701454214084E-5
120-124	1.0623697792980731E-4
125-129	1.095568834901138E-4
130-134	4.315877228398422E-5
135-139	3.651896116337126E-5
140-144	5.3948465354980285E-5
145-149	7.552785149697239E-5
150	1.1619669461072676E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4097071E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.354635984987056
#Duplication Level	Percentage of total
1	35.95778874528639
2	8.831352645986014
3	5.195034466696784
4	3.5215857988008517
5	2.6348258265738123
6	2.0594030250630575
7	1.691203494438143
8	1.417900596964565
9	1.2600828686263026
>10	15.333988369846658
>50	5.0768669842873555
>100	9.996400670472301
>500	3.339484432819353
>1k	3.3886032802305057
>5k	0.2407544816711745
>10k+	0.05472431223659865
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	8.299763900766197E-6	0.0	0.0	2.1164397946953802E-4	2.0749409751915494E-5
2	4.149881950383098E-6	4.149881950383099E-5	0.0	8.299763900766197E-6	0.0024484303507260282	2.904917365268169E-5
3	8.299763900766197E-6	4.149881950383099E-5	0.0	1.6599527801532394E-5	0.0045482706176198755	4.149881950383099E-5
4	6.224822925574647E-5	4.149881950383099E-5	0.0	1.6599527801532394E-5	0.006822405926429814	4.979858340459718E-5
5	7.884775705727887E-5	4.5648701454214084E-5	0.0	1.6599527801532394E-5	0.008013422046189763	5.394846535498028E-5
6	1.0374704875957747E-4	4.5648701454214084E-5	0.0	2.0749409751915494E-5	0.00964432565269032	5.394846535498028E-5
7	1.2449645851149294E-4	5.394846535498028E-5	0.0	2.0749409751915494E-5	0.011271079377240496	5.394846535498028E-5
8	2.489929170229859E-4	5.809834730536338E-5	0.0	2.0749409751915494E-5	0.015001823250634901	5.809834730536338E-5
9	3.278406740802648E-4	5.809834730536338E-5	0.0	2.0749409751915494E-5	0.017898440852002304	5.809834730536338E-5
10-11	3.797141984600535E-4	6.0173288280554926E-5	0.0	2.0749409751915494E-5	0.03249772555345004	9.752222583400282E-5
12-13	4.253628999142676E-4	6.432317023093802E-5	0.0	2.0749409751915494E-5	0.04183081005986163	1.1619669461072676E-4
14-15	5.083605389219296E-4	6.639811120612957E-5	0.0	2.2824350727107042E-5	0.04854946893753187	1.3279622241225915E-4
16-17	5.872082959792085E-4	6.847305218132113E-5	0.0	2.489929170229859E-5	0.052923444513235655	1.3694610436264224E-4
18-19	6.992551086395521E-4	7.469787510689577E-5	0.0	2.489929170229859E-5	0.056371996414004005	1.390210453378338E-4
20-21	0.0010333206056453916	9.752222583400282E-5	0.0	2.697423267749014E-5	0.059326712362676774	1.4109598631302536E-4
22-23	0.0013507865748496985	9.959716680919436E-5	0.0	2.904917365268169E-5	0.062387250301084315	1.4939575021379154E-4
24-25	0.0014898076201875323	1.0374704875957747E-4	4.149881950383098E-6	2.904917365268169E-5	0.06522161967319597	1.5562057313936618E-4
26-27	0.0015686553772448113	1.0374704875957747E-4	4.149881950383098E-6	4.149881950383099E-5	0.0676410008502693	1.722201009408986E-4
28-29	0.0017159761864834113	1.1204681266034366E-4	4.149881950383098E-6	4.5648701454214084E-5	0.07045877069457944	1.8259480581685634E-4
30-31	0.002027217332762144	1.1204681266034366E-4	4.149881950383098E-6	6.224822925574647E-5	0.07358363180321792	1.8674468776723943E-4
32-33	0.0021973624927278507	1.1619669461072676E-4	4.149881950383098E-6	7.469787510689578E-5	0.07648024940458531	2.3446833019664506E-4
34-35	0.0023052594234378113	1.1619669461072676E-4	4.149881950383098E-6	9.959716680919436E-5	0.07927104501621796	2.406931531222197E-4
36-37	0.0023384584790408763	1.1827163558591831E-4	4.149881950383098E-6	1.2449645851149294E-4	0.08175267442254705	2.448430350726028E-4
38-39	0.002361282829767983	1.224215175363014E-4	4.149881950383098E-6	1.4524586826340845E-4	0.0844148236937178	2.5521773994856056E-4
40-41	0.0023903320034206646	1.2449645851149294E-4	4.149881950383098E-6	1.4524586826340845E-4	0.08724711812485425	2.655924448245183E-4
42-43	0.0024048565902470053	1.265713994866845E-4	4.149881950383098E-6	1.5354563216417465E-4	0.08993001680577695	2.738922087252845E-4
44-45	0.0024276809409741126	1.2864634046187606E-4	4.149881950383098E-6	1.5562057313936618E-4	0.09295528074760621	2.738922087252845E-4
46-47	0.002458805055601986	1.2864634046187606E-4	4.149881950383098E-6	1.5769551411455774E-4	0.09803681119585031	2.780420906756676E-4
48-49	0.0024795544653539014	1.3694610436264227E-4	4.149881950383098E-6	1.597704550897493E-4	0.10083590657138372	2.780420906756676E-4
50-51	0.002494079052180242	1.514706911889831E-4	4.149881950383098E-6	1.6184539606494083E-4	0.1040064163814764	2.946416184772E-4
52-53	0.002523128225832924	1.5354563216417465E-4	4.149881950383098E-6	1.6184539606494083E-4	0.1065087951975574	2.9879150042758307E-4
54-55	0.002554252340460797	1.5354563216417465E-4	4.149881950383098E-6	1.7014515996570704E-4	0.10828701961329656	3.029413823779662E-4
56-57	0.0026351750384932676	1.5562057313936618E-4	4.149881950383098E-6	1.7636998289128166E-4	0.11048023222407403	3.029413823779662E-4
58-59	0.0026870485628730564	1.597704550897493E-4	4.149881950383098E-6	1.8674468776723943E-4	0.11258629731389347	3.029413823779662E-4
60-61	0.002794945493583017	1.6184539606494083E-4	4.149881950383098E-6	1.9296951069281408E-4	0.11460313994177965	3.1124114627873237E-4
62-63	0.0028634185457643378	1.7014515996570704E-4	4.149881950383098E-6	1.9504445166800563E-4	0.11717399181004198	3.1124114627873237E-4
64-65	0.0028841679555162533	1.7014515996570704E-4	4.149881950383098E-6	2.1371892044472955E-4	0.12004986000165746	3.1124114627873237E-4
66-67	0.002898692542342594	1.7844492386647322E-4	4.149881950383098E-6	2.282435072710704E-4	0.12239454330362391	3.1331608725392393E-4
68-69	0.0029256667750200845	1.9504445166800563E-4	4.149881950383098E-6	2.282435072710704E-4	0.12429311429592418	3.195409101794986E-4
70-71	0.0029671655945239152	2.0541915654396337E-4	4.149881950383098E-6	2.282435072710704E-4	0.1263473058613638	3.2369079212988167E-4
72-73	0.002992064886226214	2.2409362532068732E-4	4.149881950383098E-6	2.282435072710704E-4	0.12808195651662396	3.2369079212988167E-4
74-75	0.0030128142959781295	2.2616856629587885E-4	4.149881950383098E-6	2.3239338922145352E-4	0.1298830052830902	3.2369079212988167E-4
76-77	0.0030314887647548538	2.3239338922145352E-4	4.149881950383098E-6	2.3239338922145352E-4	0.1314537356013102	3.2369079212988167E-4
78-79	0.003046013351581194	2.4276809409741126E-4	4.149881950383098E-6	2.5521773994856056E-4	0.13302654086050542	3.2369079212988167E-4
80-81	0.0030626128793827265	2.510678579981775E-4	4.149881950383098E-6	2.572926809237521E-4	0.13465536952603077	3.257657331050732E-4
82-83	0.0030916620530354083	2.572926809237521E-4	4.149881950383098E-6	2.572926809237521E-4	0.13637757053543975	3.278406740802648E-4
84-85	0.003143535577415197	2.614425628741352E-4	4.149881950383098E-6	2.572926809237521E-4	0.13821181835750912	3.278406740802648E-4
86-87	0.003172584751067879	2.738922087252845E-4	4.149881950383098E-6	2.572926809237521E-4	0.13974312479720047	3.278406740802648E-4
88-89	0.0031871093378942196	2.738922087252845E-4	4.149881950383098E-6	2.572926809237521E-4	0.14134497923004835	3.278406740802648E-4
90-91	0.0031974840427701776	2.738922087252845E-4	4.149881950383098E-6	2.572926809237521E-4	0.14347179372961966	3.278406740802648E-4
92-93	0.00321408357057171	2.738922087252845E-4	4.149881950383098E-6	2.572926809237521E-4	0.14528529214193708	3.278406740802648E-4
94-95	0.003265957094951499	2.780420906756676E-4	4.149881950383098E-6	2.572926809237521E-4	0.14683942293235555	3.278406740802648E-4
96-97	0.003332355206157628	2.780420906756676E-4	4.149881950383098E-6	2.572926809237521E-4	0.14823378326768427	3.278406740802648E-4
98-99	0.0033987533173637577	2.780420906756676E-4	4.149881950383098E-6	2.593676218989436E-4	0.14954929584595572	3.278406740802648E-4
100-101	0.0035149500119744845	2.821919726260507E-4	4.149881950383098E-6	2.6351750384932674E-4	0.15088763277495426	3.2991561505545634E-4
102-103	0.0035232497758752508	2.821919726260507E-4	4.149881950383098E-6	2.655924448245183E-4	0.15204752478008635	3.319905560306479E-4
104-105	0.0035460741266023577	2.863418545764338E-4	4.149881950383098E-6	2.780420906756676E-4	0.15310574467743404	3.319905560306479E-4
106-107	0.00358964788708138	2.9256667750200845E-4	4.149881950383098E-6	2.863418545764338E-4	0.1541390652830794	3.319905560306479E-4
108-109	0.0036166221197588702	2.946416184772E-4	4.149881950383098E-6	2.904917365268169E-4	0.15531140693406265	3.340654970058394E-4
110-111	0.003653971057312318	2.946416184772E-4	4.149881950383098E-6	2.904917365268169E-4	0.15654599681430162	3.3614043798103096E-4
112-113	0.0037162192865680646	2.946416184772E-4	4.149881950383098E-6	2.904917365268169E-4	0.15777436187161503	3.402903199314141E-4
114-115	0.003759793047047087	2.946416184772E-4	4.149881950383098E-6	2.9671655945239157E-4	0.1591085489186632	3.402903199314141E-4
116-117	0.003815816453377259	2.946416184772E-4	4.149881950383098E-6	3.070912643283493E-4	0.16010037070480476	3.402903199314141E-4
118-119	0.0038739148006826226	2.987915004275831E-4	4.149881950383098E-6	3.091662053035408E-4	0.16101126979291383	3.402903199314141E-4
120-121	0.003981811731392583	3.029413823779662E-4	4.149881950383098E-6	3.257657331050732E-4	0.16197404240540272	3.402903199314141E-4
122-123	0.0041664814781846304	3.029413823779662E-4	4.149881950383098E-6	3.4444020188179714E-4	0.16296793913251945	3.402903199314141E-4
124-125	0.004234954530365952	3.070912643283493E-4	4.149881950383098E-6	3.4859008383218026E-4	0.1639016625713557	3.402903199314141E-4
126-127	0.004266078644993825	3.070912643283493E-4	4.149881950383098E-6	3.527399657825634E-4	0.16475446331215937	3.402903199314141E-4
128-129	0.004317952169373614	3.070912643283493E-4	4.149881950383098E-6	3.527399657825634E-4	0.16548484253542683	3.402903199314141E-4
130-131	0.00444659850983549	3.1124114627873237E-4	4.149881950383098E-6	3.527399657825634E-4	0.16626087046014845	3.4444020188179714E-4
132-133	0.004502621916165662	3.1124114627873237E-4	4.149881950383098E-6	3.6103972968332956E-4	0.1669912496834159	3.4444020188179714E-4
134-135	0.0046022190829748556	3.1124114627873237E-4	4.149881950383098E-6	3.651896116337127E-4	0.16786064995202116	3.4859008383218026E-4
136-137	0.004751614833188648	3.1331608725392393E-4	4.149881950383098E-6	3.651896116337127E-4	0.1686325279947924	3.548149067577549E-4
138-139	0.0048366874131715015	3.195409101794986E-4	4.149881950383098E-6	3.6933949358409574E-4	0.16942723038829077	3.5688984773294644E-4
>>END_MODULE
