##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.4d.S.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24097071
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.74344645455043	37.0	35.0	38.0	33.0	39.0
2	35.3252081134674	35.0	34.0	37.0	33.0	39.0
3	35.455411240644146	35.0	34.0	37.0	33.0	39.0
4	35.53168345646655	36.0	35.0	38.0	33.0	39.0
5	35.45021554694345	36.0	35.0	38.0	33.0	39.0
6	35.399067795417956	35.0	35.0	37.0	33.0	39.0
7	35.4423039215015	35.0	34.0	38.0	33.0	39.0
8	35.56470614208673	36.0	35.0	38.0	33.0	39.0
9	35.56017691942726	36.0	35.0	38.0	33.0	39.0
10-14	35.5175414057584	35.8	34.8	37.8	33.0	39.0
15-19	35.48876604131681	36.0	35.0	38.0	33.0	39.0
20-24	35.461307392919245	36.0	34.8	38.0	33.0	39.0
25-29	35.45275916728635	36.0	34.0	38.0	33.0	39.0
30-34	35.43905115273138	36.0	34.0	38.0	33.0	39.0
35-39	35.41048293379722	36.0	34.0	38.0	33.0	39.0
40-44	35.39598222539162	36.0	34.0	38.0	33.0	39.0
45-49	35.36711360480284	36.0	34.0	38.0	32.6	39.0
50-54	35.34886307136664	36.0	34.0	38.0	32.0	39.0
55-59	35.31802502470113	36.0	34.0	38.0	32.0	39.0
60-64	35.279219129993024	36.0	34.0	38.0	32.0	39.0
65-69	35.242522819474615	36.0	34.0	38.0	32.0	39.0
70-74	35.22410904628201	36.0	34.0	38.0	32.0	39.0
75-79	35.16901381914839	36.0	34.0	38.0	32.0	39.0
80-84	35.092225623603795	36.0	34.0	38.0	32.0	39.0
85-89	35.07947018955126	35.8	34.0	38.0	32.0	39.0
90-94	35.0330321971496	35.2	34.0	38.0	32.0	39.0
95-99	34.96727988227283	35.2	34.0	37.6	31.0	39.0
100-104	34.90285834324014	35.0	34.0	37.4	31.0	39.0
105-109	34.85020657489867	35.0	34.0	37.2	31.0	39.0
110-114	34.777936057041956	35.0	34.0	37.0	31.0	39.0
115-119	34.7157799551655	35.0	34.0	37.0	30.4	39.0
120-124	34.64652514822237	35.0	34.0	37.0	30.0	39.0
125-129	34.565683107295484	35.0	34.0	37.0	30.0	39.0
130-134	34.489411098967174	35.0	34.0	37.0	30.0	39.0
135-139	34.40609936369445	35.0	34.0	37.0	30.0	39.0
140-144	34.30762494744693	35.0	34.0	37.0	29.0	39.0
145-149	34.2020832158398	35.0	34.0	37.0	29.0	39.0
150	34.13774400216524	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	15.0
21	253.0
22	2000.0
23	9867.0
24	31260.0
25	75066.0
26	148495.0
27	257248.0
28	381027.0
29	503154.0
30	643631.0
31	834123.0
32	1107199.0
33	1532643.0
34	2287933.0
35	3868689.0
36	1.0226146E7
37	2188313.0
38	8.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.818745025069646	18.27549912601411	14.890938404920664	33.01481744399558
2	24.793175036613377	24.97528848635396	32.86228983801224	17.369246639020425
3	19.838328069000585	28.892183618498695	30.068866046002025	21.200622266498694
4	23.722169387308522	33.739519628754884	21.74781325083036	20.790497733106236
5	25.76834753070197	34.8284320530076	21.675497407962986	17.72772300832744
6	19.129055145332806	39.0622204665455	21.926818408760134	19.88190597936156
7	20.533740386954083	20.661560900907833	37.05529190663878	21.74940680549931
8	21.470240096815086	23.351161641180372	28.272942383744482	26.90565587826006
9	23.58627735296128	23.259283254798895	27.942478984271574	25.211960407968252
10-14	23.184679167024075	28.875345887473213	25.219919881549092	22.720055063953623
15-19	23.24192097869488	27.725449288006825	26.70439656338316	22.328233169915134
20-24	23.130000351689873	27.666770657055977	26.670799216399093	22.53242977485505
25-29	23.222312786479318	27.256565746102503	26.684518628840827	22.836602838577353
30-34	23.044775026807198	27.012412421410055	26.921617154217625	23.021195397565123
35-39	23.360909713881824	26.801546129818014	26.925449985186994	22.91209417111316
40-44	23.529890416972254	26.469851045382235	26.97259845397808	23.02766008366743
45-49	23.417912492352286	26.291228506568288	27.07898648761088	23.211872513468546
50-54	23.613264231269138	26.3049523845031	26.815999561241277	23.265783822986485
55-59	23.645166667766386	26.318585358361602	26.848080416080442	23.188167557791566
60-64	23.352897951788414	26.28541535193219	26.9258093649639	23.435877331315496
65-69	23.5484793981808	26.234586767827512	26.91078264242156	23.30615119157013
70-74	23.74030935129004	26.217925821773115	26.62577455990398	23.415990267032868
75-79	23.548189736420664	26.327520054200775	26.89583559761267	23.228454611765887
80-84	23.843262006800668	26.32765742368986	26.58408619169142	23.244994377818053
85-89	23.591585443584997	26.385782344846515	26.81218438227923	23.210447829289258
90-94	23.60937310596794	26.135417038859206	26.84412557858173	23.411084276591126
95-99	23.58782442895238	26.359943081879123	26.873495123120982	23.178737366047518
100-104	23.839946356965957	26.13860331822071	26.817779638031524	23.20367068678181
105-109	23.627720564046975	26.3202668905279	26.754047410990324	23.297965134434804
110-114	23.71935825727533	26.419614234443678	26.637606703320916	23.22342080496007
115-119	23.846288206562534	26.492476201775727	26.792633013364984	22.868602578296755
120-124	23.799846245212507	26.618306653271734	26.917220162709572	22.66462693880619
125-129	23.80239407519694	26.67727708483741	26.754563656304953	22.7657651836607
130-134	23.95653231050363	26.688070097813966	26.76180105042642	22.59359654125599
135-139	23.718480142254634	26.720926373167924	27.019580927491145	22.541012557086297
140-144	23.75828166004076	26.86062052935811	26.803586211784825	22.577511598816304
145-149	23.899106952201056	27.5276022958941	26.23631068820606	22.336980063698782
150	23.673246428995455	27.51687539120418	26.185020577812136	22.624857601988225
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	3.5
2	9.0
3	21.5
4	36.0
5	36.0
6	27.0
7	19.0
8	15.0
9	9.5
10	5.5
11	7.5
12	9.5
13	18.0
14	23.5
15	24.0
16	50.0
17	103.0
18	177.5
19	303.5
20	467.0
21	679.0
22	1079.5
23	1784.0
24	2868.5
25	4631.0
26	7258.5
27	11398.0
28	17303.5
29	24752.0
30	35175.0
31	49947.0
32	71153.0
33	97939.5
34	133024.0
35	186958.0
36	263904.0
37	357626.5
38	475305.0
39	620966.5
40	792817.0
41	982132.0
42	1177303.0
43	1370911.5
44	1521714.5
45	1613686.0
46	1641145.5
47	1610956.5
48	1561591.5
49	1505062.5
50	1394155.0
51	1232782.0
52	1049729.0
53	871218.5
54	729481.5
55	617680.5
56	506610.0
57	401975.0
58	331243.0
59	270311.0
60	202609.5
61	136647.5
62	84440.5
63	51672.0
64	32163.0
65	16535.0
66	9508.5
67	5414.5
68	3177.5
69	2106.5
70	1287.0
71	1043.0
72	911.5
73	740.0
74	610.5
75	375.5
76	129.5
77	55.5
78	15.5
79	3.0
80	1.0
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.6599527801532394E-5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.03181050510246661
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.299763900766196E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.06282755277601995
85-89	1.6599527801532393E-6
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	8.299763900766196E-7
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.03427636495738424
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4097071E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.33725202679334
#Duplication Level	Percentage of total
1	25.67342331173745
2	8.570905990130797
3	5.392026176712606
4	3.815678055491306
5	2.813121072171555
6	2.3344530344268772
7	1.8329727959800757
8	1.5760186742888669
9	1.3724241486060087
>10	16.937241302773767
>50	6.169356749315962
>100	12.62737366932216
>500	4.011771944295751
>1k	5.461949277213259
>5k	0.8437297580286002
>10k+	0.5675540395049731
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	27636	0.1146861375807873	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.697423267749014E-4	0.0	0.0	0.0	0.0021330393224969126	0.0
2	0.0014981073840882985	0.0	0.0	0.0	0.00886829772796868	0.0
3	0.0023280837741649183	0.0	0.0	0.0	0.0126446903028173	0.0
4	0.0057475865012805915	0.0	0.0	0.0	0.018678618658674324	0.0
5	0.005905282015395149	0.0	0.0	0.0	0.022683254740794016	0.0
6	0.007013300496147437	0.0	0.0	0.0	0.029551309368678044	0.0
7	0.008984494422579408	0.0	0.0	0.0	0.040473798662086356	0.0
8	0.016333935356707876	4.149881950383098E-6	0.0	0.0	0.055243228523499804	0.0
9	0.01693151835756304	8.299763900766197E-6	0.0	0.0	0.06583372726087747	0.0
10-11	0.017655672757904892	1.2449645851149295E-5	0.0	0.0	0.10838039195718019	0.0
12-13	0.01849602385285747	1.2449645851149295E-5	0.0	0.0	0.1284907198887367	0.0
14-15	0.02172670695123071	1.4524586826340845E-5	0.0	0.0	0.13787982780147845	0.0
16-17	0.025305980133436136	1.6599527801532394E-5	0.0	0.0	0.14494915170395606	0.0
18-19	0.0322134586398488	2.2824350727107042E-5	0.0	0.0	0.15164706117187438	0.0
20-21	0.03817061417962374	2.697423267749014E-5	0.0	0.0	0.1561538329699904	0.0
22-23	0.04054642159621806	2.904917365268169E-5	0.0	0.0	0.1607996258134443	0.0
24-25	0.04627325868774674	4.357376047902253E-5	0.0	0.0	0.16518397609402402	0.0
26-27	0.05174902792127724	5.809834730536338E-5	0.0	0.0	0.16883379726938597	0.0
28-29	0.06449124044992854	5.809834730536338E-5	0.0	2.074940975191549E-6	0.17189848508974387	0.0
30-31	0.0900130144447846	6.224822925574647E-5	0.0	4.149881950383098E-6	0.1751395428929931	0.0
32-33	0.11066490197086609	6.639811120612957E-5	0.0	4.149881950383098E-6	0.17777471793148636	0.0
34-35	0.12581197108976439	6.847305218132113E-5	0.0	4.149881950383098E-6	0.1808124315191668	0.0
36-37	0.12821890262098662	7.054799315651268E-5	0.0	4.149881950383098E-6	0.1828686980255816	0.0
38-39	0.1306465835619607	7.262293413170422E-5	0.0	4.149881950383098E-6	0.1843688803506451	0.0
40-41	0.13223598834895745	7.677281608208731E-5	4.149881950383098E-6	4.149881950383098E-6	0.18590433667228684	0.0
42-43	0.1325617540820625	8.299763900766196E-5	4.149881950383098E-6	4.149881950383098E-6	0.1876576617963237	0.0
44-45	0.1334705782291964	9.337234388361971E-5	4.149881950383098E-6	4.149881950383098E-6	0.18896279966971918	0.0
46-47	0.13481099009917014	9.959716680919436E-5	4.149881950383098E-6	4.149881950383098E-6	0.19037168459187426	0.0
48-49	0.13586713505554265	9.959716680919436E-5	4.149881950383098E-6	4.149881950383098E-6	0.19151082718725443	0.0
50-51	0.1368382074319323	9.959716680919436E-5	4.149881950383098E-6	4.149881950383098E-6	0.1926209206089819	0.0
52-53	0.13783417910002424	1.0789693070996056E-4	6.224822925574648E-6	4.149881950383098E-6	0.1938409859023945	0.0
54-55	0.13834253963894616	1.141217536355352E-4	8.299763900766197E-6	4.149881950383098E-6	0.19493447979632048	0.0
56-57	0.1391164926226926	1.224215175363014E-4	8.299763900766197E-6	4.149881950383098E-6	0.19599684957561853	0.0
58-59	0.1392700382548568	1.2864634046187606E-4	8.299763900766197E-6	4.149881950383098E-6	0.19699489618468569	2.074940975191549E-6
60-61	0.13955638010943322	1.307212814370676E-4	8.299763900766197E-6	4.149881950383098E-6	0.1977978983420848	4.149881950383098E-6
62-63	0.13979084843962986	1.3279622241225915E-4	8.299763900766197E-6	4.149881950383098E-6	0.1987585960135985	4.149881950383098E-6
64-65	0.13983234725913368	1.390210453378338E-4	8.299763900766197E-6	4.149881950383098E-6	0.19977531709144236	4.149881950383098E-6
66-67	0.13991326995716616	1.6807021899051548E-4	8.299763900766197E-6	4.149881950383098E-6	0.20057001948494074	4.149881950383098E-6
68-69	0.13999211771422343	1.7844492386647322E-4	1.0374704875957745E-5	4.149881950383098E-6	0.2013896211701414	4.149881950383098E-6
70-71	0.14011868911371012	1.7844492386647322E-4	1.2449645851149295E-5	4.149881950383098E-6	0.20222582238314357	4.149881950383098E-6
72-73	0.14019131204784183	1.7844492386647322E-4	1.2449645851149295E-5	4.149881950383098E-6	0.2028649042035026	4.149881950383098E-6
74-75	0.14021206145759374	1.8051986484166478E-4	1.2449645851149295E-5	6.224822925574648E-6	0.20362640754139788	4.149881950383098E-6
76-77	0.14025563521807277	1.8259480581685634E-4	1.2449645851149295E-5	8.299763900766197E-6	0.2044563839314745	4.149881950383098E-6
78-79	0.14030958368342777	1.9089456971762252E-4	1.2449645851149295E-5	8.299763900766197E-6	0.20564117522830888	4.149881950383098E-6
80-81	0.14036560708975793	1.9504445166800563E-4	1.2449645851149295E-5	1.0374704875957745E-5	0.20663299701445043	8.299763900766197E-6
82-83	0.14040503096828655	1.971193926431972E-4	1.2449645851149295E-5	1.2449645851149295E-5	0.20761236915474085	8.299763900766197E-6
84-85	0.14044860472876558	1.9919433361838872E-4	1.2449645851149295E-5	1.2449645851149295E-5	0.20857306682625454	8.299763900766197E-6
86-87	0.14049840331217017	1.9919433361838872E-4	1.2449645851149295E-5	1.2449645851149295E-5	0.20940719309828154	8.299763900766197E-6
88-89	0.14052745248582285	1.9919433361838872E-4	1.2449645851149295E-5	1.2449645851149295E-5	0.21035336618296888	8.299763900766197E-6
90-91	0.14054612695459956	2.0334421556877181E-4	1.2449645851149295E-5	1.2449645851149295E-5	0.2110546962325836	8.299763900766197E-6
92-93	0.1405668763643515	2.095690384943465E-4	1.2449645851149295E-5	1.2449645851149295E-5	0.2115692815944311	1.0374704875957745E-5
94-95	0.14059800047897938	2.157938614199211E-4	1.2449645851149295E-5	1.2449645851149295E-5	0.21225193717526913	1.2449645851149295E-5
96-97	0.14061252506580574	2.3239338922145352E-4	1.2449645851149295E-5	1.2449645851149295E-5	0.21293459275610715	1.2449645851149295E-5
98-99	0.14062497471165686	2.3239338922145352E-4	2.0749409751915494E-5	1.2449645851149295E-5	0.21354877528476385	1.2449645851149295E-5
100-101	0.14066024870823512	2.3446833019664508E-4	2.0749409751915494E-5	1.2449645851149295E-5	0.21412975875781748	1.6599527801532394E-5
102-103	0.1406706234131111	2.3861821214702815E-4	2.4899291702298594E-5	1.2449645851149295E-5	0.21473149164062305	1.6599527801532394E-5
104-105	0.14071212223261492	2.4276809409741126E-4	2.904917365268169E-5	1.2449645851149295E-5	0.21542659686731222	1.6599527801532394E-5
106-107	0.14073702152431722	2.489929170229859E-4	2.904917365268169E-5	1.2449645851149295E-5	0.2160905779793735	1.6599527801532394E-5
108-109	0.1407598458750443	2.53142798973369E-4	2.904917365268169E-5	1.2449645851149295E-5	0.2167068354490054	1.8674468776723945E-5
110-111	0.14080134469454814	2.614425628741352E-4	2.904917365268169E-5	1.2449645851149295E-5	0.2173023435088854	2.0749409751915494E-5
112-113	0.14082624398625043	2.655924448245183E-4	2.904917365268169E-5	1.2449645851149295E-5	0.21797877426679782	2.0749409751915494E-5
114-115	0.140842843514052	2.655924448245183E-4	2.904917365268169E-5	1.2449645851149295E-5	0.2186220059691072	2.0749409751915494E-5
116-117	0.14085944304185352	2.655924448245183E-4	2.904917365268169E-5	1.2449645851149295E-5	0.2191158419212028	2.0749409751915494E-5
118-119	0.1408677428057543	2.6766738579970986E-4	2.904917365268169E-5	1.2449645851149295E-5	0.21976737338741292	2.0749409751915494E-5
120-121	0.14087189268770467	2.738922087252845E-4	2.904917365268169E-5	1.2449645851149295E-5	0.22040853014874712	2.489929170229859E-5
122-123	0.140886417274531	2.8011703165085915E-4	2.904917365268169E-5	1.2449645851149295E-5	0.2210040382086271	2.489929170229859E-5
124-125	0.14090094186135735	2.863418545764338E-4	2.904917365268169E-5	1.2449645851149295E-5	0.22161614579630862	2.489929170229859E-5
126-127	0.1409133915072085	2.9256667750200845E-4	3.319905560306479E-5	1.6599527801532394E-5	0.2222531526756924	2.904917365268169E-5
128-129	0.14092169127110926	3.029413823779662E-4	3.319905560306479E-5	1.6599527801532394E-5	0.22293165837458007	4.979858340459718E-5
130-131	0.14092584115305964	3.091662053035408E-4	3.319905560306479E-5	1.6599527801532394E-5	0.22376993452855742	5.602340633017183E-5
132-133	0.14094244068086118	3.1331608725392393E-4	3.319905560306479E-5	1.6599527801532394E-5	0.22446711469622177	6.224822925574647E-5
134-135	0.1409631900906131	3.236907921298817E-4	3.319905560306479E-5	1.6599527801532394E-5	0.22512072110340714	6.224822925574647E-5
136-137	0.14097148985451385	3.319905560306479E-4	3.7348937553447884E-5	1.6599527801532394E-5	0.2257203790452375	6.224822925574647E-5
138-139	0.14098808938231538	3.340654970058394E-4	3.7348937553447884E-5	1.6599527801532394E-5	0.2265005568519095	6.432317023093802E-5
>>END_MODULE
