##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.control.4d.S.3.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24129992
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.7066270888113	37.0	35.0	38.0	33.0	39.0
2	35.32356890130755	35.0	34.0	37.0	33.0	39.0
3	35.4608212882955	35.0	34.0	37.0	33.0	39.0
4	35.518602824236325	36.0	35.0	38.0	33.0	39.0
5	35.44465340063105	36.0	35.0	37.0	33.0	39.0
6	35.39319702219545	35.0	35.0	37.0	33.0	39.0
7	35.432479463731276	35.0	34.0	38.0	33.0	39.0
8	35.54982724403721	36.0	35.0	38.0	33.0	39.0
9	35.544291436151326	36.0	35.0	38.0	33.0	39.0
10-14	35.50497477993362	35.8	34.6	37.8	33.0	39.0
15-19	35.478208770230836	36.0	34.6	38.0	33.0	39.0
20-24	35.452098682834205	36.0	34.4	38.0	33.0	39.0
25-29	35.444732348025646	36.0	34.0	38.0	33.0	39.0
30-34	35.42932441917097	36.0	34.0	38.0	33.0	39.0
35-39	35.40063630356777	36.0	34.0	38.0	33.0	39.0
40-44	35.385473124069	36.0	34.0	38.0	32.6	39.0
45-49	35.35741788061927	36.0	34.0	38.0	32.0	39.0
50-54	35.33905291804489	36.0	34.0	38.0	32.0	39.0
55-59	35.30759155659894	36.0	34.0	38.0	32.0	39.0
60-64	35.26691830647934	36.0	34.0	38.0	32.0	39.0
65-69	35.23122174263464	36.0	34.0	38.0	32.0	39.0
70-74	35.21291098646033	36.0	34.0	38.0	32.0	39.0
75-79	35.15730988224116	36.0	34.0	38.0	32.0	39.0
80-84	35.08113277451563	36.0	34.0	38.0	32.0	39.0
85-89	35.06523982270694	35.8	34.0	38.0	32.0	39.0
90-94	35.01959551416345	35.2	34.0	38.0	31.6	39.0
95-99	34.95377882429467	35.2	34.0	37.4	31.0	39.0
100-104	34.88826337779142	35.0	34.0	37.4	31.0	39.0
105-109	34.83317375322793	35.0	34.0	37.0	31.0	39.0
110-114	34.76170627822836	35.0	34.0	37.0	31.0	39.0
115-119	34.69733907081279	35.0	34.0	37.0	30.0	39.0
120-124	34.624307301883896	35.0	34.0	37.0	30.0	39.0
125-129	34.54407304403582	35.0	34.0	37.0	30.0	39.0
130-134	34.46403484924487	35.0	34.0	37.0	30.0	39.0
135-139	34.37508086202432	35.0	34.0	37.0	29.8	39.0
140-144	34.27651031960558	35.0	34.0	37.0	29.0	39.0
145-149	34.16533048995624	35.0	34.0	37.0	29.0	39.0
150	34.08758962705002	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	17.0
21	251.0
22	2184.0
23	10196.0
24	33029.0
25	78141.0
26	153116.0
27	265527.0
28	385538.0
29	506567.0
30	648787.0
31	838698.0
32	1115615.0
33	1546144.0
34	2295283.0
35	3886823.0
36	1.0189329E7
37	2174741.0
38	4.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.587261139053055	19.303562939580043	15.157568852802308	30.95160706856459
2	24.415433888889556	26.264539427304385	31.642403016229885	17.67762366757617
3	19.87298213774791	29.91014667555629	29.15048210542299	21.0663890812728
4	23.054122852589426	34.5800238972313	21.600632938460983	20.765220311718295
5	25.048603414373282	35.29092757262414	21.83633131747412	17.824137695528453
6	19.006433984727387	39.375450269523505	21.956621452671847	19.66149429307726
7	20.578672384143353	20.894316914817047	36.962395180238765	21.564615520800835
8	21.67116756607296	23.39943585559415	28.281592467995846	26.64780411033704
9	23.688250704766084	23.117185451201145	27.997327972591123	25.19723587144165
10-14	23.146785129477042	28.631469915116426	25.38933705406948	22.832407901337056
15-19	23.12263841612546	27.530118534643528	26.876739122002196	22.47050392722882
20-24	22.976812464492788	27.5868241466674	26.7766963209499	22.659667067889917
25-29	23.096599451835704	27.226789797526664	26.728702603796968	22.94790814684066
30-34	22.939190365251676	26.979906997068216	26.924487169328525	23.156415468351586
35-39	23.242156068680007	26.720187888997227	26.975083953612582	23.06257208871018
40-44	23.38898993418647	26.39934401967477	27.010812933547594	23.20085311259117
45-49	23.30869152380987	26.235194773375802	27.118178903664784	23.33793479914954
50-54	23.48009432311926	26.26270707073788	26.813367869166367	23.443830736976494
55-59	23.538847422742617	26.2436887670746	26.86674906481527	23.350714745367508
60-64	23.211638860054325	26.22243306172667	26.95955473172142	23.606373346497588
65-69	23.427173950161276	26.157614971443007	26.94231643342443	23.472894644971287
70-74	23.588398205851043	26.139314095089627	26.645009248241774	23.627278450817553
75-79	23.394956147477846	26.27646230696191	26.927117314644093	23.40146423091615
80-84	23.713823045187695	26.23373262491302	26.623220265306475	23.42922406459281
85-89	23.47387887196778	26.317457980383217	26.842457748721305	23.366205398927697
90-94	23.478931116098174	26.07263359225316	26.88800642785128	23.560428863797387
95-99	23.429145770127068	26.280026118533318	26.900843564307852	23.389984547031762
100-104	23.7063766950275	26.069188916432296	26.819116226810184	23.405318161730015
105-109	23.5063244115456	26.236149601707286	26.771228933685514	23.486297053061598
110-114	23.593837080426717	26.33631043060437	26.650661964579186	23.41919052438973
115-119	23.73762494409447	26.40925782321022	26.81285928316926	23.04025794952605
120-124	23.699150200291406	26.54980098252665	26.945909695667613	22.80513912151433
125-129	23.650467849305546	26.616937129527436	26.786687703833472	22.94590731733355
130-134	23.822119160438337	26.635713983127008	26.779386638664338	22.762780217770317
135-139	23.613169867607084	26.62883104146906	27.069500064484064	22.688499026439793
140-144	23.618175256751016	26.799905279703367	26.838633017366938	22.743286446178683
145-149	23.818284286463392	27.55321056405925	26.16221102824174	22.46629412123562
150	23.555113486983338	27.508160798395625	26.124654330594062	22.812071384026982
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	3.0
2	12.5
3	25.5
4	39.5
5	40.0
6	28.5
7	23.0
8	13.5
9	8.0
10	8.5
11	7.5
12	7.5
13	12.5
14	16.5
15	26.0
16	61.0
17	110.0
18	180.5
19	277.0
20	429.5
21	668.5
22	1055.5
23	1768.0
24	2897.5
25	4654.0
26	7204.5
27	11089.5
28	16800.0
29	24218.0
30	34705.5
31	50084.0
32	71974.0
33	99800.0
34	135946.5
35	191456.0
36	270264.0
37	365556.5
38	484626.0
39	628172.0
40	796774.0
41	982323.0
42	1174269.0
43	1368466.5
44	1519480.0
45	1610089.0
46	1636157.5
47	1601355.0
48	1551831.5
49	1500445.0
50	1392022.0
51	1232677.5
52	1049939.5
53	873285.5
54	733475.0
55	620002.5
56	507502.5
57	404543.0
58	335406.0
59	274395.0
60	207234.5
61	140793.0
62	86805.0
63	52514.5
64	32667.0
65	16795.0
66	9323.5
67	5274.5
68	3262.5
69	2079.5
70	1086.0
71	910.0
72	801.5
73	652.5
74	559.5
75	350.0
76	113.5
77	43.5
78	13.5
79	2.0
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.144220188717841E-6
2	4.144220188717841E-6
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.03078492524987161
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	4.97306422646141E-6
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	8.288440377435682E-7
80-84	0.0601558425713527
85-89	1.6576880754871363E-6
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	8.288440377435682E-7
125-129	0.0
130-134	8.288440377435682E-7
135-139	0.0
140-144	0.0
145-149	0.03142479284700964
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4129992E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.05509878819149
#Duplication Level	Percentage of total
1	25.40017864369669
2	8.548506587923443
3	5.364582539686028
4	3.750289827570467
5	2.884711717219648
6	2.315898500489549
7	1.8612403290674175
8	1.5845092594839503
9	1.3668890774086315
>10	17.202705984734276
>50	5.935349065605676
>100	12.70991596230672
>500	4.004102232524338
>1k	5.4768058655559955
>5k	0.9457278049790145
>10k+	0.6485866017480786
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	29595	0.12264819648510451	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.8595119302153105E-4	0.0	4.144220188717841E-6	0.0	0.0025362627554953187	0.0
2	0.0015582267909579083	0.0	4.144220188717841E-6	0.0	0.010460011756323832	0.0
3	0.002345628626814298	0.0	4.144220188717841E-6	0.0	0.01420638680692476	0.0
4	0.005524245511560883	0.0	4.144220188717841E-6	0.0	0.01996685286924256	0.0
5	0.005685870098920878	0.0	4.144220188717841E-6	0.0	0.023924583149468097	0.0
6	0.006556156338551625	4.144220188717841E-6	4.144220188717841E-6	0.0	0.03190220701274994	0.0
7	0.008284296157246965	8.288440377435682E-6	4.144220188717841E-6	0.0	0.04243267051228198	0.0
8	0.014181521485792452	8.288440377435682E-6	4.144220188717841E-6	0.0	0.05787817915563337	0.0
9	0.014720270110325772	1.6576880754871364E-5	4.144220188717841E-6	0.0	0.06804809549874695	0.0
10-11	0.015397850111181138	1.6576880754871364E-5	4.144220188717841E-6	0.0	0.11506841775993958	0.0
12-13	0.016232910479207784	1.6576880754871364E-5	4.144220188717841E-6	0.0	0.13722963521910825	0.0
14-15	0.01929134497848155	1.6576880754871364E-5	4.144220188717841E-6	0.0	0.14757982514043105	0.0
16-17	0.02268753342313582	1.6576880754871364E-5	4.144220188717841E-6	0.0	0.15508500790219906	0.0
18-19	0.02884584462357053	2.2793211037948127E-5	4.144220188717841E-6	0.0	0.16224000405802042	0.0
20-21	0.03448612830041552	2.486532113230705E-5	4.144220188717841E-6	0.0	0.1668110789261762	0.0
22-23	0.03696851619345751	3.315376150974273E-5	4.144220188717841E-6	0.0	0.1716577444368817	0.0
24-25	0.04257564610879275	3.315376150974273E-5	4.144220188717841E-6	0.0	0.1760257525157903	0.0
26-27	0.04752591712421621	3.729798169846057E-5	4.144220188717841E-6	0.0	0.17948824848346406	0.0
28-29	0.059233339157344106	3.729798169846057E-5	4.144220188717841E-6	0.0	0.18275596610226807	0.0
30-31	0.0834956762521927	4.144220188717841E-5	4.144220188717841E-6	0.0	0.18679865289636233	0.0
32-33	0.1040012777459686	4.5586422075896254E-5	4.144220188717841E-6	0.0	0.1899275391388443	0.0
34-35	0.11918984473761947	4.973064226461409E-5	4.144220188717841E-6	0.0	0.19334859290463088	0.0
36-37	0.12170953061235992	5.3874862453331936E-5	4.144220188717841E-6	0.0	0.19554295749455697	0.0
38-39	0.1241670531842696	6.009119273640869E-5	4.144220188717841E-6	0.0	0.1972110061205159	0.0
40-41	0.12573356841560496	6.630752301948546E-5	4.144220188717841E-6	0.0	0.1990075255723251	0.0
42-43	0.1260547454802306	6.837963311384438E-5	4.144220188717841E-6	0.0	0.2008392708957384	0.0
44-45	0.12675511869212389	7.04517432082033E-5	4.144220188717841E-6	0.0	0.20218407034697733	0.0
46-47	0.12809991814336283	7.04517432082033E-5	4.144220188717841E-6	0.0	0.20370085493604806	0.0
48-49	0.12935976108073305	7.459596339692114E-5	4.144220188717841E-6	0.0	0.2048591644787947	0.0
50-51	0.13033365282508175	7.459596339692114E-5	4.144220188717841E-6	0.0	0.20596981548937104	6.2163302830767615E-6
52-53	0.13131168878961919	7.459596339692114E-5	4.144220188717841E-6	0.0	0.20714884613306128	8.288440377435682E-6
54-55	0.1318297163132089	8.288440377435683E-5	4.144220188717841E-6	0.0	0.2082470644830715	8.288440377435682E-6
56-57	0.1326171181490653	8.702862396307467E-5	4.144220188717841E-6	0.0	0.20928104742015663	8.288440377435682E-6
58-59	0.13280153594746322	9.117284415179251E-5	4.144220188717841E-6	0.0	0.21029430925629813	8.288440377435682E-6
60-61	0.13310406402123964	9.94612845292282E-5	4.144220188717841E-6	0.0	0.21110657641328684	8.288440377435682E-6
62-63	0.13338379888397808	9.94612845292282E-5	6.2163302830767615E-6	0.0	0.21213641513018322	8.288440377435682E-6
64-65	0.1334231689757709	1.0360550471794603E-4	8.288440377435682E-6	0.0	0.21310409054424884	8.288440377435682E-6
66-67	0.1335143418199227	1.0774972490666387E-4	8.288440377435682E-6	0.0	0.21379410320567035	8.288440377435682E-6
68-69	0.13355163980162116	1.1189394509538171E-4	8.288440377435682E-6	0.0	0.21470168742699958	8.288440377435682E-6
70-71	0.13367182218709397	1.1603816528409956E-4	1.2432660566153525E-5	0.0	0.21550773825370517	8.288440377435682E-6
72-73	0.13374641815049088	1.1811027537845847E-4	1.6576880754871364E-5	0.0	0.2160817127498426	8.288440377435682E-6
74-75	0.13377957191200063	1.2225449556717632E-4	1.6576880754871364E-5	0.0	0.21682767238381181	8.288440377435682E-6
76-77	0.13381479778360472	1.326150460389709E-4	1.6576880754871364E-5	0.0	0.2176606606417441	8.288440377435682E-6
78-79	0.13386660053596372	1.388313763220477E-4	1.6576880754871364E-5	0.0	0.21881275385420768	1.2432660566153525E-5
80-81	0.13392669172870011	1.4297559651076553E-4	1.6576880754871364E-5	0.0	0.2196270931212907	1.2432660566153525E-5
82-83	0.13395155704983241	1.4504770660512444E-4	1.6576880754871364E-5	0.0	0.22052846101233686	1.2432660566153525E-5
84-85	0.13398678292143654	1.4711981669948335E-4	1.6576880754871364E-5	0.0	0.22147748743555323	1.4504770660512445E-5
86-87	0.13403236934351243	1.5126403688820123E-4	1.6576880754871364E-5	0.0	0.22240372064773167	2.0721100943589205E-5
88-89	0.13408002787568268	1.5748036717127796E-4	1.6576880754871364E-5	0.0	0.22333409808009883	2.0721100943589205E-5
90-91	0.13411525374728678	1.6162458735999581E-4	1.6576880754871364E-5	0.0	0.224022038631426	2.2793211037948127E-5
92-93	0.13413390273813602	1.6162458735999581E-4	1.6576880754871364E-5	0.0	0.22460430156794084	2.900954132102489E-5
94-95	0.13417120071983446	1.6784091764307258E-4	1.6576880754871364E-5	0.0	0.22541035239464646	3.108165141538381E-5
96-97	0.13418156127030625	1.802735782092261E-4	1.6576880754871364E-5	0.0	0.22617910523965362	3.522587160410165E-5
98-99	0.13419399393087242	1.8856201858666178E-4	2.0721100943589205E-5	0.0	0.2267862334973008	3.729798169846057E-5
100-101	0.134214715031816	1.906341286810207E-4	2.0721100943589205E-5	0.0	0.22733741478240027	3.937009179281949E-5
102-103	0.1342250755822878	1.906341286810207E-4	2.4865321132307046E-5	0.0	0.22785337019589563	4.144220188717841E-5
104-105	0.13424786879332573	1.906341286810207E-4	3.315376150974273E-5	0.0	0.22852266175637356	4.144220188717841E-5
106-107	0.13428102255483548	1.9477834886973854E-4	3.315376150974273E-5	0.0	0.22915465533515306	4.144220188717841E-5
108-109	0.13430795998606215	1.989225690584564E-4	3.315376150974273E-5	0.0	0.22984466799657455	4.144220188717841E-5
110-111	0.1343556185182324	2.0513889934153316E-4	3.315376150974273E-5	0.0	0.23047873368544838	4.765853217025518E-5
112-113	0.13437426750908166	2.0928311953025098E-4	3.315376150974273E-5	0.0	0.23107550139262376	5.3874862453331936E-5
114-115	0.13438877227974216	2.134273397189688E-4	3.315376150974273E-5	0.0	0.23167226909979913	5.3874862453331936E-5
116-117	0.134405349160497	2.1757155990768668E-4	3.315376150974273E-5	4.144220188717841E-6	0.23223381093537038	5.801908264204978E-5
118-119	0.13442192604125192	2.2378789019076342E-4	3.315376150974273E-5	4.144220188717841E-6	0.23280364121131908	6.216330283076762E-5
120-121	0.13443435870181805	2.2378789019076342E-4	3.315376150974273E-5	8.288440377435682E-6	0.233443923230476	6.630752301948546E-5
122-123	0.13443850292200676	2.300042204738402E-4	3.315376150974273E-5	8.288440377435682E-6	0.234038618827557	6.630752301948546E-5
124-125	0.13445300769266727	2.3829266085127588E-4	3.522587160410165E-5	8.288440377435682E-6	0.23466232396595904	6.837963311384438E-5
126-127	0.13446958457342215	2.4450899113435265E-4	3.729798169846057E-5	8.288440377435682E-6	0.2354020672696452	7.04517432082033E-5
128-129	0.1344778730137996	2.4450899113435265E-4	3.729798169846057E-5	8.288440377435682E-6	0.23614181057333133	7.04517432082033E-5
130-131	0.1344882335642714	2.527974315117883E-4	3.729798169846057E-5	8.288440377435682E-6	0.23704732268456616	7.04517432082033E-5
132-133	0.1345276036560642	2.527974315117883E-4	3.729798169846057E-5	1.4504770660512445E-5	0.23769796525419487	7.04517432082033E-5
134-135	0.13455039686710216	2.590137617948651E-4	3.729798169846057E-5	1.6576880754871364E-5	0.23833410305316305	7.04517432082033E-5
136-137	0.13455868530747958	2.61085871889224E-4	3.729798169846057E-5	1.6576880754871364E-5	0.23899303406316919	7.04517432082033E-5
138-139	0.1345773342983288	2.6937431226665965E-4	3.729798169846057E-5	1.6576880754871364E-5	0.23968097461449636	7.04517432082033E-5
>>END_MODULE
