##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.2d.R.1.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22878051
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.84395982857106	36.0	34.0	38.0	30.0	39.0
2	34.70038877000493	35.0	34.0	37.0	31.0	39.0
3	34.92503028339259	35.0	34.0	38.0	31.0	39.0
4	34.87021626099181	36.0	34.0	38.0	31.0	39.0
5	34.74851782610328	35.0	34.0	37.0	31.0	39.0
6	34.742283204106855	35.0	34.0	37.0	31.0	39.0
7	34.75308241947708	35.0	34.0	37.0	32.0	39.0
8	34.891794759964476	35.0	34.0	37.0	32.0	39.0
9	34.87250531087635	35.0	34.0	37.0	32.0	39.0
10-14	34.84999218683445	35.0	34.0	37.0	31.2	39.0
15-19	34.849990936727956	35.0	34.0	37.0	31.0	39.0
20-24	34.858079947457064	35.0	34.0	37.0	31.0	39.0
25-29	34.84591293200632	35.0	34.0	37.0	31.0	39.0
30-34	34.836632561051644	35.0	34.0	37.0	31.0	39.0
35-39	34.830921637511864	35.0	34.0	37.0	31.0	39.0
40-44	34.76685993050719	35.0	34.0	37.0	31.0	39.0
45-49	34.68181776498356	35.0	34.0	37.0	31.0	39.0
50-54	34.64225633555935	35.0	34.0	37.0	31.0	39.0
55-59	34.63386999181006	35.0	34.0	37.0	31.0	39.0
60-64	34.60821047212457	35.0	34.0	37.0	31.0	39.0
65-69	34.59017314892777	35.0	34.0	37.0	31.0	39.0
70-74	34.56509123963401	35.0	34.0	37.0	30.2	39.0
75-79	34.54651009388868	35.0	34.0	37.0	30.0	39.0
80-84	34.52700456870211	35.0	34.0	37.0	30.0	39.0
85-89	34.5066048414701	35.0	34.0	37.0	30.0	39.0
90-94	34.48014737793879	35.0	34.0	37.0	30.0	39.0
95-99	34.455430228737576	35.0	34.0	37.0	30.0	39.0
100-104	34.41771458591468	35.0	34.0	37.0	30.0	39.0
105-109	34.369468518100604	35.0	34.0	37.0	30.0	39.0
110-114	34.338718486115795	35.0	34.0	37.0	30.0	39.0
115-119	34.29457564370322	35.0	34.0	37.0	30.0	39.0
120-124	34.259535788253984	35.0	34.0	37.0	30.0	39.0
125-129	34.21715633031853	35.0	34.0	37.0	29.8	39.0
130-134	34.16242721899693	35.0	34.0	37.0	29.0	39.0
135-139	34.12101660233208	35.0	34.0	37.0	29.0	39.0
140-144	34.08516482457357	35.0	34.0	37.0	29.0	39.0
145-149	34.023575618395114	35.0	34.0	37.0	29.0	39.0
150	33.98903070895331	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	23.0
22	396.0
23	4013.0
24	23247.0
25	86041.0
26	210380.0
27	373085.0
28	540300.0
29	698437.0
30	864742.0
31	1079920.0
32	1376331.0
33	1810633.0
34	2510992.0
35	3954074.0
36	8342555.0
37	1002871.0
38	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.141319693895944	11.202929175715399	14.008142854620157	38.6476082757685
2	22.189733476795706	13.755305968946246	37.24725072217826	26.807709832079784
3	20.792467534908482	18.137278835354522	28.501674204100137	32.56857942563686
4	25.790821471379154	26.096219056470122	22.262666138532985	25.850293333617742
5	25.32094853384544	31.35176978627326	24.502189763155023	18.82509191672628
6	18.75064772838187	36.92514010493298	25.92719626964401	18.39701589704114
7	15.928307366391614	24.078727339746802	40.75286042280183	19.240104871059746
8	18.40251783949726	24.224440333075925	31.625747272699694	25.74729455472712
9	19.69169634324705	22.949236579777043	32.656012456317804	24.7030546206581
10-14	20.325483060636078	29.15330853239765	27.014762185717938	23.506446221248336
15-19	20.410989939527504	27.913828303097088	27.94600772991619	23.72917402745922
20-24	20.56871571645772	28.081890318698456	27.645060103646664	23.704333861197163
25-29	20.52805588201465	28.288899165829058	27.529905337853233	23.65313961430306
30-34	20.522267631017236	28.00045581208268	27.562081557579997	23.915194999320086
35-39	20.598899897248106	28.011681843938796	27.6149509761561	23.774467282657
40-44	20.656021373554694	28.060772959710384	27.52001650790496	23.763189158829963
45-49	20.741077460463117	27.84297974628927	27.480434441020705	23.935508352226904
50-54	20.840211184717987	27.836634904522334	27.57588813178675	23.747265778972928
55-59	20.848219531327604	27.856332326562306	27.483765881672124	23.811682260437966
60-64	20.7913929155755	27.781432381890525	27.483867792412365	23.94330691012161
65-69	20.908399907243563	27.692563188223968	27.562494893561674	23.836542010970792
70-74	21.015245271446396	27.660663557738207	27.527263662581387	23.79682750823401
75-79	21.035221550572075	27.52771726107661	27.52795154747477	23.90910964087654
80-84	21.082198832527894	27.530937722334453	27.522693112245022	23.864170332892634
85-89	21.060662610979925	27.534662739347976	27.57827413229745	23.826400517374648
90-94	21.05009795192728	27.429806538846048	27.538979747719782	23.98111576150689
95-99	21.13208975254214	27.352600872484057	27.640889333092673	23.87442004188113
100-104	21.188288997827183	27.407258896279256	27.51816045866774	23.886291647225818
105-109	21.148839595753348	27.240569810827107	27.603891019670222	24.00669957374932
110-114	21.225549011462164	27.2300027046943	27.66713616863403	23.877312115209502
115-119	21.260776181416542	27.257366268260665	27.61954649711107	23.862311053211727
120-124	21.22367363704906	27.14589791830918	27.631769960578893	23.99865848406286
125-129	21.27657348044685	27.079780706595084	27.68397621735268	23.95966959560539
130-134	21.339443176774036	27.17833182982269	27.586260644893816	23.895964348509455
135-139	21.326448820872507	27.005987144515753	27.684201489831857	23.983362544779887
140-144	21.376327508148215	26.96029646629292	27.808356092447532	23.855019933111326
145-149	21.38756160721014	27.578658522832672	27.43539165392226	23.59838821603493
150	21.247189167062423	27.07232995184676	27.57697622672956	24.103504654361252
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	2.0
10	2.5
11	4.5
12	12.0
13	17.0
14	35.0
15	75.5
16	157.0
17	313.0
18	619.5
19	1182.0
20	2216.5
21	3679.0
22	5772.5
23	9036.0
24	14344.5
25	22708.5
26	34002.5
27	48014.0
28	65467.5
29	91266.0
30	123060.0
31	155677.0
32	188085.0
33	224276.5
34	262051.0
35	305916.5
36	373430.0
37	463667.5
38	572751.5
39	708187.0
40	866134.0
41	1025372.5
42	1187895.5
43	1353752.0
44	1488401.0
45	1569260.0
46	1592010.5
47	1554368.0
48	1468071.5
49	1345030.5
50	1187486.0
51	1015507.5
52	843053.5
53	674302.5
54	521167.0
55	392525.5
56	294430.5
57	221301.0
58	166824.0
59	125155.0
60	87908.0
61	61233.0
62	44470.0
63	30213.0
64	20859.5
65	16700.0
66	15026.0
67	11370.0
68	6669.5
69	4402.5
70	3713.0
71	2986.5
72	2187.0
73	1290.5
74	634.5
75	196.0
76	55.5
77	33.5
78	20.0
79	3.5
80	1.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.4530913494335686E-4
2	1.8795307345018157E-4
3	6.993602733030012E-5
4	2.1855008540718788E-5
5	2.6226010248862542E-5
6	8.742003416287515E-5
7	2.2729208882347538E-4
8	1.0490404099545017E-4
9	9.616203757916266E-5
10-14	1.8882727379181032E-4
15-19	3.4880593630987186E-4
20-24	2.395308936062779E-4
25-29	3.426865339184706E-4
30-34	8.82942345045039E-4
35-39	9.808527833074592E-4
40-44	0.001242238685454456
45-49	0.0010848826239612806
50-54	6.36417848705731E-4
55-59	4.003837564659682E-4
60-64	2.6226010248862544E-4
65-69	3.050959192284343E-4
70-74	3.147121229863505E-4
75-79	3.015991178619193E-4
80-84	3.2345412640263807E-4
85-89	3.680383438257044E-4
90-94	4.0999996022388443E-4
95-99	2.74498907271428E-4
100-104	4.57206778671837E-4
105-109	2.543922994139667E-4
110-114	2.168016847239304E-4
115-119	2.1417908369904414E-4
120-124	2.2816628916510413E-4
125-129	1.1364604441173769E-4
130-134	1.7396586798412155E-4
135-139	1.44243056368744E-4
140-144	1.6172706320131903E-4
145-149	2.054370802827566E-4
150	2.0106607857461284E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.2878051E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.3304577147417
#Duplication Level	Percentage of total
1	39.73865688173325
2	10.89115699788286
3	6.342151000483262
4	4.4578193661007015
5	3.3533422773198396
6	2.687745981546308
7	2.1755437583633412
8	1.9305751962078874
9	1.5950220924797305
>10	17.793032157829387
>50	4.356714811390586
>100	4.116126915934945
>500	0.3304818389428553
>1k	0.23163072378522984
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.748400683257503E-5	0.0	0.0	1.966950768664691E-4	3.05970119570063E-5
2	0.0	1.0490404099545018E-4	0.0	0.0	0.0021898718557800227	5.6823022205868845E-5
3	1.748400683257503E-5	1.2675904953616897E-4	0.0	0.0	0.003282622282815962	7.867803074658763E-5
4	4.3710017081437576E-5	1.3987205466060024E-4	0.0	4.371001708143758E-6	0.004668229824297533	1.0053303928730642E-4
5	5.245202049772509E-5	1.3987205466060024E-4	0.0	1.3113005124431273E-5	0.005743496244500898	1.1801704611988145E-4
6	7.867803074658763E-5	1.3987205466060024E-4	0.0	1.3113005124431273E-5	0.006831875669828693	1.1801704611988145E-4
7	8.742003416287515E-5	1.5735606149317526E-4	0.0	1.3113005124431273E-5	0.007963965112237925	1.2675904953616897E-4
8	1.0053303928730642E-4	1.5735606149317526E-4	0.0	1.3113005124431273E-5	0.010223772995348249	1.2675904953616897E-4
9	1.1801704611988145E-4	1.5735606149317526E-4	0.0	1.3113005124431273E-5	0.01359818631403523	1.355010529524565E-4
10-11	1.7265456747167843E-4	1.5735606149317526E-4	0.0	1.3113005124431273E-5	0.018082834066590725	2.1855008540718786E-4
12-13	2.1855008540718786E-4	1.5735606149317526E-4	0.0	1.3113005124431273E-5	0.021706394482641903	2.6444560334269734E-4
14-15	2.3384859138569103E-4	1.6828356576353468E-4	0.0	1.529850597850315E-5	0.026169187226656676	2.731876067589849E-4
16-17	2.5133259821826605E-4	1.9888057772054097E-4	0.0	1.748400683257503E-5	0.030909538579138578	2.7537310761305674E-4
18-19	2.7537310761305674E-4	2.2073558626125976E-4	0.0	1.748400683257503E-5	0.034996425176252996	2.7537310761305674E-4
20-21	3.3656713152706937E-4	2.7318760675898484E-4	0.0	1.748400683257503E-5	0.038641840600844884	2.9504261529970363E-4
22-23	4.1087416056551323E-4	2.906716135915599E-4	0.0	1.748400683257503E-5	0.042897010763722834	3.2345412640263807E-4
24-25	4.939231930202446E-4	3.037846187159912E-4	0.0	1.748400683257503E-5	0.04761987810937217	3.3438163067299746E-4
26-27	5.267057058313228E-4	3.05970119570063E-4	0.0	1.748400683257503E-5	0.05189034677822862	3.5623663921371626E-4
28-29	5.616737194964728E-4	3.05970119570063E-4	0.0	1.966950768664691E-5	0.05640122054103298	3.7153514519221934E-4
30-31	6.163112408482697E-4	3.05970119570063E-4	0.0	2.6226010248862546E-5	0.060992957835438	4.0650315885736943E-4
32-33	6.578357570756355E-4	3.0815562042413487E-4	0.0	2.6226010248862546E-5	0.06518693397440192	4.523986767928789E-4
34-35	7.146587792815043E-4	3.1034112127820677E-4	0.0	2.6226010248862546E-5	0.06892632593571892	4.5676967850102266E-4
36-37	7.408847895303669E-4	3.1034112127820677E-4	0.0	2.6226010248862546E-5	0.07249524883041829	4.633261810632383E-4
38-39	7.736673023414451E-4	3.168976238404224E-4	0.0	3.278251281107818E-5	0.07710665563250996	4.6769718277138206E-4
40-41	8.151918185688108E-4	3.190831246944943E-4	0.0	3.496801366515006E-5	0.08077392606564257	4.7643918618766955E-4
42-43	8.173773194228827E-4	3.2563962725670997E-4	0.0	3.496801366515006E-5	0.08417893639628655	4.808101878958133E-4
44-45	8.63272837358392E-4	3.365671315270693E-4	0.0	3.496801366515006E-5	0.08835761402927199	4.895521913121008E-4
46-47	9.157248578561172E-4	3.4749463579742876E-4	2.185500854071879E-6	3.496801366515006E-5	0.09255814667079815	4.982941947283884E-4
48-49	9.419508681049798E-4	3.496801366515006E-4	4.371001708143758E-6	3.496801366515006E-5	0.09644178168848386	5.135927007068915E-4
50-51	9.659913774997704E-4	3.496801366515006E-4	4.371001708143758E-6	3.496801366515006E-5	0.10050681327705757	5.201492032691072E-4
52-53	0.0010140723962893516	3.5405113835964435E-4	4.371001708143758E-6	4.1524516227365695E-5	0.10484940347409838	5.332622083935384E-4
54-55	0.0010577824133707894	3.584221400677881E-4	4.371001708143758E-6	4.3710017081437576E-5	0.10834401933975932	5.332622083935384E-4
56-57	0.0010774519210574362	3.6716414348407565E-4	4.371001708143758E-6	4.808101878958133E-5	0.11274998906156823	5.529317160801854E-4
58-59	0.0011036779313062987	3.8901915202479444E-4	4.371001708143758E-6	5.245202049772509E-5	0.11733954085511916	5.660447212046166E-4
60-61	0.001138645944971449	3.9994665629515384E-4	4.371001708143758E-6	5.245202049772509E-5	0.12086693923359118	5.726012237668323E-4
62-63	0.001188912464615102	4.0650315885736943E-4	4.371001708143758E-6	5.245202049772509E-5	0.12460414569405409	5.726012237668323E-4
64-65	0.00122169497742618	4.0650315885736943E-4	4.371001708143758E-6	6.556502562215636E-5	0.1296242411558572	5.726012237668323E-4
66-67	0.0012304369808424677	4.3491466996030387E-4	4.371001708143758E-6	7.430702903844388E-5	0.13373953926407456	5.76972225474976E-4
68-69	0.0012828890013401929	4.545841776469508E-4	4.371001708143758E-6	7.649252989251576E-5	0.1367118204256123	5.857142288912636E-4
70-71	0.0013309700201297743	4.633261810632383E-4	4.371001708143758E-6	8.304903245473139E-5	0.13950051951540804	5.944562323075511E-4
72-73	0.0013637525329408522	4.720681844795258E-4	4.371001708143758E-6	8.304903245473139E-5	0.142175572560792	6.010127348697667E-4
74-75	0.0014140190525845056	4.7643918618766955E-4	4.371001708143758E-6	8.960553501694704E-5	0.14540574282311025	6.031982357238386E-4
76-77	0.001459914570520015	4.895521913121008E-4	4.371001708143758E-6	9.616203757916266E-5	0.147982448330061	6.053837365779103E-4
78-79	0.001499253585893309	4.939231930202446E-4	4.371001708143758E-6	1.333155520983846E-4	0.15051325831907622	6.075692374319822E-4
80-81	0.0015407781021206746	5.026651964365321E-4	4.371001708143758E-6	1.355010529524565E-4	0.15316645635591947	6.11940239140126E-4
82-83	0.0015779316166398966	5.114071998528196E-4	4.371001708143758E-6	1.355010529524565E-4	0.1558699209124064	6.11940239140126E-4
84-85	0.0016216416337213341	5.157782015609634E-4	4.371001708143758E-6	1.355010529524565E-4	0.15827615735273953	6.14125739994198E-4
86-87	0.0016653516508027717	5.267057058313228E-4	4.371001708143758E-6	1.355010529524565E-4	0.1604376176974166	6.250532442645573E-4
88-89	0.0016893921601975623	5.354477092476103E-4	4.371001708143758E-6	1.355010529524565E-4	0.16252040001134713	6.29424245972701E-4
90-91	0.001711247168738281	5.42004211809826E-4	4.371001708143758E-6	1.3987205466060024E-4	0.16535499461907835	6.29424245972701E-4
92-93	0.0017352876781330717	5.441897126638978E-4	4.371001708143758E-6	1.464285572228159E-4	0.16813932270716592	6.29424245972701E-4
94-95	0.0018095947071715157	5.485607143720416E-4	4.371001708143758E-6	1.4861405807688776E-4	0.17121213690799097	6.316097468267729E-4
96-97	0.0018970147413343908	5.573027177883291E-4	4.371001708143758E-6	1.4861405807688776E-4	0.1735746633312427	6.359807485349167E-4
98-99	0.0019472812609780438	5.704157229127604E-4	4.371001708143758E-6	1.5079955893095964E-4	0.17571645416823312	6.381662493889886E-4
100-101	0.00197787827293505	5.813432271831198E-4	4.371001708143758E-6	1.529850597850315E-4	0.17822540914870763	6.425372510971323E-4
102-103	0.0019953622797676254	5.813432271831198E-4	4.371001708143758E-6	1.5517056063910339E-4	0.18018143241310197	6.425372510971323E-4
104-105	0.0020325157942868476	5.878997297453354E-4	4.371001708143758E-6	1.5735606149317526E-4	0.18210030216297707	6.425372510971323E-4
106-107	0.0020915243173467877	6.031982357238386E-4	4.371001708143758E-6	1.5954156234724713E-4	0.18389241286331603	6.425372510971323E-4
108-109	0.0022073558626125975	6.031982357238386E-4	4.371001708143758E-6	1.639125640553909E-4	0.18557524852095136	6.425372510971323E-4
110-111	0.002277291889942898	6.075692374319822E-4	4.371001708143758E-6	1.6609806490946278E-4	0.18714006713246684	6.425372510971323E-4
112-113	0.0023494134181272696	6.075692374319822E-4	4.371001708143758E-6	1.6609806490946278E-4	0.18894091983622208	6.469082528052761E-4
114-115	0.00240842194118721	6.14125739994198E-4	4.371001708143758E-6	1.6828356576353468E-4	0.1907570710459558	6.512792545134198E-4
116-117	0.0024412044539982884	6.250532442645573E-4	4.371001708143758E-6	1.748400683257503E-4	0.19245739071042373	6.512792545134198E-4
118-119	0.002504583978766373	6.556502562215636E-4	4.371001708143758E-6	1.748400683257503E-4	0.1940987018518317	6.534647553674917E-4
120-121	0.0025854475103670328	6.687632613459949E-4	4.371001708143758E-6	1.748400683257503E-4	0.1956241814479739	6.600212579297074E-4
122-123	0.0027362470692979925	6.709487622000667E-4	4.371001708143758E-6	1.748400683257503E-4	0.19714747554326195	6.600212579297074E-4
124-125	0.00283896560943937	6.731342630541386E-4	4.371001708143758E-6	1.748400683257503E-4	0.1987844156829618	6.622067587837793E-4
126-127	0.0028761191239585924	6.731342630541386E-4	4.371001708143758E-6	1.7702556917982218E-4	0.20025744325860625	6.643922596378511E-4
128-129	0.0029242001427481736	6.731342630541386E-4	4.371001708143758E-6	1.7921107003389405E-4	0.20143979922065913	6.643922596378511E-4
130-131	0.002991950669224402	6.862472681785699E-4	4.371001708143758E-6	1.7921107003389405E-4	0.20270520421516675	6.643922596378511E-4
132-133	0.003064072197408774	6.906182698867137E-4	4.371001708143758E-6	1.8139657088796592E-4	0.2037826561362242	6.643922596378511E-4
134-135	0.0032105007546315897	6.971747724489293E-4	4.371001708143758E-6	1.857675725961097E-4	0.2048775920641142	6.643922596378511E-4
136-137	0.0033263322998973994	7.015457741570731E-4	4.371001708143758E-6	1.8795307345018157E-4	0.20603372201591824	6.66577760491923E-4
138-139	0.0034312363408928494	7.168442801355763E-4	4.371001708143758E-6	1.8795307345018157E-4	0.20712210144124604	6.687632613459949E-4
>>END_MODULE
