##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.2d.R.1.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22878051
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.81579042725274	37.0	35.0	38.0	33.0	39.0
2	35.35759899302611	35.0	34.0	37.0	33.0	39.0
3	35.534218233887145	35.0	35.0	38.0	33.0	39.0
4	35.58394659580049	36.0	35.0	38.0	33.0	39.0
5	35.49573086448667	36.0	35.0	38.0	33.0	39.0
6	35.443732379126175	35.0	35.0	37.0	33.0	39.0
7	35.476528223492465	35.0	34.0	38.0	33.0	39.0
8	35.565664662606096	36.0	35.0	38.0	33.0	39.0
9	35.567977141059785	36.0	35.0	38.0	33.0	39.0
10-14	35.55761776210745	35.8	35.0	37.8	33.0	39.0
15-19	35.53054494021366	36.0	35.0	38.0	33.0	39.0
20-24	35.51785514421662	36.0	35.0	38.0	33.0	39.0
25-29	35.496673165034906	36.0	35.0	38.0	33.0	39.0
30-34	35.48036782503895	36.0	35.0	38.0	33.0	39.0
35-39	35.4520025941021	36.0	34.8	38.0	33.0	39.0
40-44	35.43725323455219	36.0	34.4	38.0	33.0	39.0
45-49	35.406728003185236	36.0	34.0	38.0	33.0	39.0
50-54	35.386612557162316	36.0	34.0	38.0	33.0	39.0
55-59	35.359968268276	36.0	34.0	38.0	32.8	39.0
60-64	35.320624715802936	36.0	34.0	38.0	32.0	39.0
65-69	35.2947600912333	36.0	34.0	38.0	32.0	39.0
70-74	35.26624057267815	36.0	34.0	38.0	32.0	39.0
75-79	35.21987907099254	36.0	34.0	38.0	32.0	39.0
80-84	35.18003919127551	36.0	34.0	38.0	32.0	39.0
85-89	35.13372139086498	35.8	34.0	38.0	32.0	39.0
90-94	35.07857645740889	35.4	34.0	38.0	32.0	39.0
95-99	35.016993178308766	35.0	34.0	37.0	32.0	39.0
100-104	34.969583265637446	35.0	34.0	37.0	31.4	39.0
105-109	34.9127349528157	35.0	34.0	37.0	31.0	39.0
110-114	34.83934471515952	35.0	34.0	37.0	31.0	39.0
115-119	34.78048070615805	35.0	34.0	37.0	31.0	39.0
120-124	34.70875520821245	35.0	34.0	37.0	30.8	39.0
125-129	34.633788682436276	35.0	34.0	37.0	30.0	39.0
130-134	34.53570645506473	35.0	34.0	37.0	30.0	39.0
135-139	34.480702232895624	35.0	34.0	37.0	30.0	39.0
140-144	34.35494551524515	35.0	34.0	37.0	30.0	39.0
145-149	34.28232720523265	35.0	34.0	37.0	29.2	39.0
150	34.213768253248496	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	231.0
22	1853.0
23	8910.0
24	28129.0
25	67323.0
26	133816.0
27	229271.0
28	337402.0
29	445120.0
30	574084.0
31	747736.0
32	1004877.0
33	1412736.0
34	2147378.0
35	3735146.0
36	1.0299879E7
37	1704147.0
38	6.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.48310784865372	18.356738517629843	15.390056609280222	31.770097024436218
2	24.20947920782238	25.411233675456007	33.72516303945646	16.65412407726515
3	19.866862784771307	29.145157513636104	29.776767260462876	21.21121244112971
4	23.64483320716437	34.02012697672542	22.211975137217763	20.123064678892447
5	25.24399477910072	35.12895831904562	22.45801008136576	17.1690368204879
6	18.948209355770736	38.846801241941456	23.02988134784733	19.175108054440475
7	20.308141633218668	20.432855053955425	38.14726175756842	21.11174155525748
8	21.594423020815295	23.17346549071489	28.492455254679488	26.739656233790328
9	23.52718769618968	23.087255116268427	28.92535295073868	24.46020423680321
10-14	23.30951093692378	29.01299066078662	25.606685639436684	22.07081276285292
15-19	23.05921426611034	28.253939988157207	27.075019633446924	21.611826112285527
20-24	23.17605376437005	28.328982219682963	26.921865852995957	21.573098162951034
25-29	23.243118917778443	28.32957143071322	26.87660500450847	21.55070464699987
30-34	23.24339079408469	28.076474696205544	27.067098504151428	21.61303600555834
35-39	23.29294679247762	28.101499800848416	27.058918848103964	21.54663455857
40-44	23.4591174174681	27.860833318894457	27.14243295587052	21.537616307766918
45-49	23.4528378313345	27.683103774880124	27.243816354810996	21.620242038974386
50-54	23.51680132193079	27.681160427520684	27.19838591145723	21.603652339091298
55-59	23.691071411633796	27.572209713143835	27.09324671056988	21.64347216465249
60-64	23.59915798771495	27.53859845840889	27.18238629680474	21.679857257071415
65-69	23.67740961568282	27.459814015274414	27.140675813168492	21.72210055587428
70-74	23.75476739692555	27.44014514173432	27.056424517980137	21.748662943359992
75-79	23.675248385450317	27.467230490919004	27.14622412547293	21.711296998157753
80-84	23.784367820493102	27.5194447289238	27.024021408117328	21.67216604246577
85-89	23.803141272829578	27.447473563198194	27.05695603178785	21.69242913218438
90-94	23.716832554633545	27.388995044979968	27.148679117366065	21.745493283020423
95-99	23.80111837323905	27.48869210930599	27.14495391237654	21.565235605078424
100-104	23.957121172603383	27.45798145130457	26.96845461180238	21.616442764289665
105-109	23.7513265443809	27.58273770785807	27.06431242766265	21.601623320098376
110-114	23.76959784162468	27.571549278792084	27.017934858332353	21.64091802125088
115-119	23.849146940008133	27.564390865288306	27.033036162040204	21.55342603266336
120-124	23.8162394165482	27.472443347556137	27.217656783788097	21.49366045210757
125-129	23.779100720742367	27.568828396049238	27.170487631852474	21.48158325135592
130-134	23.838304934707296	27.491041594593803	27.21726688987251	21.453386580826393
135-139	23.762574880176636	27.480666950169834	27.32690035527939	21.429857814374135
140-144	23.77402428205095	27.639184823917034	27.29420263990145	21.292588254130564
145-149	23.888548897806025	28.20540351098964	26.767786294383207	21.13826129682113
150	23.51913194004157	28.068658470951043	26.93277937006085	21.479430218946536
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	2.0
9	3.0
10	4.5
11	5.0
12	7.0
13	11.5
14	23.0
15	38.5
16	60.5
17	117.0
18	191.0
19	312.5
20	494.0
21	798.0
22	1254.5
23	2197.0
24	3707.0
25	6105.0
26	9732.5
27	15518.5
28	25903.5
29	39581.0
30	54032.5
31	71635.5
32	95074.5
33	125815.0
34	169170.0
35	233537.5
36	327135.5
37	442589.0
38	577925.5
39	750533.0
40	954505.0
41	1147589.5
42	1324930.0
43	1488979.0
44	1605824.5
45	1662745.5
46	1664745.0
47	1613548.5
48	1514980.5
49	1375153.5
50	1195330.0
51	1001581.0
52	816239.5
53	648132.5
54	506285.5
55	383571.0
56	283861.5
57	210170.5
58	154100.5
59	111471.5
60	77324.5
61	52158.0
62	35877.5
63	25471.0
64	18070.0
65	13009.5
66	9798.0
67	7388.0
68	6386.5
69	5032.5
70	3152.0
71	2303.0
72	1621.5
73	1218.5
74	1017.0
75	628.0
76	235.5
77	71.5
78	21.0
79	2.5
80	2.0
81	1.5
82	0.0
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.11851534031460985
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	8.742003416287515E-7
40-44	8.742003416287515E-7
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	8.742003416287515E-7
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	8.742003416287515E-7
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0198880577720541
115-119	0.0
120-124	0.0
125-129	3.496801366515006E-6
130-134	0.04872180763999521
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.2878051E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.34612224047518
#Duplication Level	Percentage of total
1	29.943223043870244
2	10.96870567317874
3	6.8838673359764835
4	5.05856177862983
5	3.8649644761263766
6	3.106061172312562
7	2.4086216011681705
8	2.0332616097363085
9	1.7956216249878594
>10	21.047212999971602
>50	5.197668665223843
>100	5.938370053103635
>500	0.9277236368718819
>1k	0.7178073475173947
>5k	0.06305770835451255
>10k+	0.04527127297072894
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	1.3113005124431273E-5	1.3113005124431273E-5	0.0	0.0	6.381662493889886E-4	0.0
2	3.496801366515006E-5	6.11940239140126E-5	0.0	0.0	0.0042704686688564515	0.0
3	5.6823022205868845E-5	6.11940239140126E-5	0.0	0.0	0.00576972225474976	0.0
4	1.0053303928730642E-4	6.11940239140126E-5	0.0	0.0	0.006993602733030012	0.0
5	1.0053303928730642E-4	6.11940239140126E-5	0.0	0.0	0.008628357371875778	0.0
6	1.1364604441173769E-4	6.11940239140126E-5	0.0	0.0	0.01013198195947723	0.0
7	1.2675904953616897E-4	8.304903245473139E-5	0.0	0.0	0.012081448721309345	0.0
8	1.8795307345018157E-4	8.304903245473139E-5	0.0	0.0	0.01704253566005251	0.0
9	1.8795307345018157E-4	9.179103587101891E-5	0.0	0.0	0.02248443278669149	0.0
10-11	2.0106607857461284E-4	1.0490404099545018E-4	0.0	2.185500854071879E-6	0.02824104203631682	0.0
12-13	2.513325982182661E-4	1.0927504270359393E-4	0.0	4.371001708143758E-6	0.033851222728719335	0.0
14-15	3.147121229863506E-4	1.2020254697395333E-4	0.0	4.371001708143758E-6	0.04083826895918713	0.0
16-17	3.824626494625788E-4	1.223880478280252E-4	0.0	6.556502562215636E-6	0.046343545610594186	2.185500854071879E-6
18-19	4.742536853335977E-4	1.3113005124431272E-4	0.0	8.742003416287515E-6	0.05375895000846007	4.371001708143758E-6
20-21	5.245202049772509E-4	1.4205755551467212E-4	0.0	8.742003416287515E-6	0.05924018615047234	4.371001708143758E-6
22-23	5.79157726329048E-4	1.5079955893095964E-4	4.371001708143758E-6	8.742003416287515E-6	0.0648394393386045	4.371001708143758E-6
24-25	6.49093753659348E-4	1.573560614931753E-4	8.742003416287515E-6	8.742003416287515E-6	0.06970217873891443	4.371001708143758E-6
26-27	6.993602733030012E-4	1.6828356576353468E-4	8.742003416287515E-6	8.742003416287515E-6	0.0734656112096262	4.371001708143758E-6
28-29	8.654583382124639E-4	1.7046906661760656E-4	8.742003416287515E-6	8.742003416287515E-6	0.07731646371450085	4.371001708143758E-6
30-31	0.0011495734492418083	1.748400683257503E-4	1.0927504270359394E-5	8.742003416287515E-6	0.0818754184960948	4.371001708143758E-6
32-33	0.0013571960303786369	1.857675725961097E-4	1.3113005124431273E-5	1.3113005124431273E-5	0.08639066326060729	4.371001708143758E-6
34-35	0.0015276650969962432	1.9232407515832532E-4	1.3113005124431273E-5	1.3113005124431273E-5	0.08918373335211116	4.371001708143758E-6
36-37	0.0015779316166398966	2.0106607857461284E-4	1.3113005124431273E-5	1.3113005124431273E-5	0.09235052408966131	4.371001708143758E-6
38-39	0.0016216416337213341	2.0325157942868472E-4	1.3113005124431273E-5	1.3113005124431273E-5	0.09544956430073523	4.371001708143758E-6
40-41	0.0016740936542190591	2.0543708028275662E-4	1.3113005124431273E-5	1.3113005124431273E-5	0.09809183483330813	4.371001708143758E-6
42-43	0.001695948662759778	2.076225811368285E-4	1.3113005124431273E-5	1.3113005124431273E-5	0.10094391344787193	4.371001708143758E-6
44-45	0.0017440296815493593	2.141790836990441E-4	1.3113005124431273E-5	1.3113005124431273E-5	0.10314252730706824	4.371001708143758E-6
46-47	0.001785554197776725	2.3166309053161916E-4	1.3113005124431273E-5	1.748400683257503E-5	0.10557936075935839	4.371001708143758E-6
48-49	0.0018423772199825939	2.360340922397629E-4	1.3113005124431273E-5	1.748400683257503E-5	0.10771022409207848	8.742003416287515E-6
50-51	0.0019035712438966064	2.360340922397629E-4	1.3113005124431273E-5	1.748400683257503E-5	0.10970340087099202	8.742003416287515E-6
52-53	0.0019472812609780438	2.4259059480197855E-4	1.3113005124431273E-5	2.1855008540718788E-5	0.11169876315075965	8.742003416287515E-6
54-55	0.0019953622797676254	2.7318760675898484E-4	1.3113005124431273E-5	2.1855008540718788E-5	0.11344060733145495	8.742003416287515E-6
56-57	0.0020827823139305006	2.863006118834161E-4	1.3113005124431273E-5	2.1855008540718788E-5	0.11539881609670334	8.742003416287515E-6
58-59	0.0021330488335741535	3.05970119570063E-4	1.3113005124431273E-5	2.1855008540718788E-5	0.11721496730643707	8.742003416287515E-6
60-61	0.0022139123651748133	3.278251281107818E-4	1.3113005124431273E-5	2.1855008540718788E-5	0.11862898635902158	8.742003416287515E-6
62-63	0.002255436881402179	3.365671315270693E-4	1.3113005124431273E-5	2.1855008540718788E-5	0.12059593712768626	1.0927504270359394E-5
64-65	0.002286033893359185	3.5623663921371626E-4	1.3113005124431273E-5	2.1855008540718788E-5	0.12222850626567797	1.3113005124431273E-5
66-67	0.0023734539275220602	3.715351451922194E-4	1.3113005124431273E-5	2.1855008540718788E-5	0.12347205625164487	1.3113005124431273E-5
68-69	0.002401865438624995	3.7809164775443505E-4	1.3113005124431273E-5	2.1855008540718788E-5	0.12483143778287756	1.3113005124431273E-5
70-71	0.0024237204471657136	3.8901915202479444E-4	1.3113005124431273E-5	2.1855008540718788E-5	0.12611432678421775	1.748400683257503E-5
72-73	0.00245431745912272	4.15245162273657E-4	1.529850597850315E-5	2.1855008540718788E-5	0.1271458831873397	1.748400683257503E-5
74-75	0.0024870999719337978	4.1743066312772883E-4	1.748400683257503E-5	2.6226010248862546E-5	0.12822770611010526	1.748400683257503E-5
76-77	0.002504583978766373	4.4584217423066327E-4	1.748400683257503E-5	2.6226010248862546E-5	0.12956304713194317	1.748400683257503E-5
78-79	0.0025329954898693076	4.5895517935509456E-4	1.748400683257503E-5	2.6226010248862546E-5	0.13132893182203326	1.748400683257503E-5
80-81	0.002554850498410026	4.6769718277138206E-4	1.748400683257503E-5	2.6226010248862546E-5	0.13276043488145034	2.1855008540718788E-5
82-83	0.002572334505242601	4.7643918618766955E-4	1.748400683257503E-5	2.6226010248862546E-5	0.13440174602285831	2.1855008540718788E-5
84-85	0.0026029315171996075	4.829956887498852E-4	1.748400683257503E-5	2.6226010248862546E-5	0.1359512661283953	2.1855008540718788E-5
86-87	0.0026160445223240387	4.895521913121008E-4	1.966950768664691E-5	2.6226010248862546E-5	0.13764065828859284	2.1855008540718788E-5
88-89	0.0026204155240321827	4.939231930202446E-4	2.4040509394790665E-5	2.6226010248862546E-5	0.1392382594129194	2.1855008540718788E-5
90-91	0.002631343028302542	5.092216989987478E-4	2.6226010248862546E-5	2.6226010248862546E-5	0.14025233180920876	2.1855008540718788E-5
92-93	0.0026357140300106857	5.22334704123179E-4	2.8411511102934423E-5	2.6226010248862546E-5	0.14127296070806034	2.6226010248862546E-5
94-95	0.002648827035135117	5.376332101016822E-4	3.05970119570063E-5	2.6226010248862546E-5	0.14224987958983043	3.05970119570063E-5
96-97	0.002653198036843261	5.507462152261134E-4	3.278251281107818E-5	2.6226010248862546E-5	0.14315686244427028	3.05970119570063E-5
98-99	0.0026597545394054763	5.638592203505448E-4	3.9339015373293815E-5	2.6226010248862546E-5	0.1440485467927316	3.496801366515006E-5
100-101	0.002672867544529908	5.638592203505448E-4	3.9339015373293815E-5	2.6226010248862546E-5	0.14482877059763527	3.496801366515006E-5
102-103	0.0026772385462380515	5.726012237668323E-4	3.9339015373293815E-5	3.05970119570063E-5	0.145733567951221	3.496801366515006E-5
104-105	0.0026837950488002673	5.747867246209042E-4	3.9339015373293815E-5	3.05970119570063E-5	0.14656405827576835	3.496801366515006E-5
106-107	0.0027143920607572733	5.988272340156948E-4	3.9339015373293815E-5	3.05970119570063E-5	0.1473661370892127	3.496801366515006E-5
108-109	0.0027143920607572733	6.11940239140126E-4	4.1524516227365695E-5	3.05970119570063E-5	0.14815291739667857	3.496801366515006E-5
110-111	0.002723134064173561	6.359807485349167E-4	4.808101878958133E-5	3.05970119570063E-5	0.14907082775538877	3.496801366515006E-5
112-113	0.0027253195650276327	6.381662493889886E-4	4.808101878958133E-5	3.05970119570063E-5	0.14983356755345986	3.496801366515006E-5
114-115	0.0027296905667357767	6.425372510971323E-4	4.808101878958133E-5	3.05970119570063E-5	0.15066842887971532	3.7153514519221934E-5
116-117	0.0027318760675898485	6.469082528052761E-4	4.808101878958133E-5	3.05970119570063E-5	0.15157541173415515	4.3710017081437576E-5
118-119	0.0027318760675898485	6.578357570756355E-4	5.245202049772509E-5	3.05970119570063E-5	0.15239278905357803	4.808101878958133E-5
120-121	0.0027340615684439203	6.709487622000667E-4	5.245202049772509E-5	3.05970119570063E-5	0.15326917489606084	4.808101878958133E-5
122-123	0.0027384325701520643	6.796907656163543E-4	5.245202049772509E-5	3.05970119570063E-5	0.1539969466804668	4.808101878958133E-5
124-125	0.002742803571860208	6.862472681785699E-4	5.245202049772509E-5	3.05970119570063E-5	0.15487551802380367	4.808101878958133E-5
126-127	0.002751545575276495	6.928037707407855E-4	5.245202049772509E-5	3.05970119570063E-5	0.15591363092948785	5.4637521351796965E-5
128-129	0.002758102077838711	7.102877775733606E-4	5.4637521351796965E-5	3.05970119570063E-5	0.1568162427822195	5.6823022205868845E-5
130-131	0.002762473079546855	7.124732784274325E-4	5.6823022205868845E-5	3.05970119570063E-5	0.15789369470327697	5.9008523059940726E-5
132-133	0.002782142587233502	7.124732784274325E-4	5.6823022205868845E-5	3.05970119570063E-5	0.1587591530414894	6.11940239140126E-5
134-135	0.0028192961017527236	7.124732784274325E-4	5.6823022205868845E-5	3.496801366515006E-5	0.15950877983443607	6.11940239140126E-5
136-137	0.0028236671034608672	7.146587792815043E-4	5.6823022205868845E-5	3.9339015373293815E-5	0.16039390768033518	6.337952476808447E-5
138-139	0.002841151110293442	7.277717844059356E-4	5.6823022205868845E-5	3.9339015373293815E-5	0.16125718051769358	6.775052647622825E-5
>>END_MODULE
