##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.2d.R.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24083933
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.743110147333496	37.0	35.0	38.0	33.0	39.0
2	35.326313231314835	35.0	34.0	37.0	33.0	39.0
3	35.48784191518885	35.0	34.0	37.0	33.0	39.0
4	35.54688982899928	36.0	35.0	38.0	33.0	39.0
5	35.4589666895353	36.0	35.0	37.0	33.0	39.0
6	35.41920420555895	35.0	35.0	37.0	33.0	39.0
7	35.442929690927144	35.0	34.0	37.0	33.0	39.0
8	35.571017159032955	36.0	35.0	38.0	33.0	39.0
9	35.52384953072241	36.0	35.0	38.0	33.0	39.0
10-14	35.51400879582251	35.8	34.8	37.8	33.0	39.0
15-19	35.48842207790563	36.0	35.0	38.0	33.0	39.0
20-24	35.474450746894206	36.0	34.8	38.0	33.0	39.0
25-29	35.45049089781141	36.0	34.4	38.0	33.0	39.0
30-34	35.434203184338706	36.0	34.0	38.0	33.0	39.0
35-39	35.403244303993034	36.0	34.0	38.0	33.0	39.0
40-44	35.38734792195278	36.0	34.0	38.0	33.0	39.0
45-49	35.354008674579845	36.0	34.0	38.0	32.8	39.0
50-54	35.327997507716034	36.0	34.0	38.0	32.4	39.0
55-59	35.306482998437176	36.0	34.0	38.0	32.0	39.0
60-64	35.26218170429224	36.0	34.0	38.0	32.0	39.0
65-69	35.23284316560755	36.0	34.0	38.0	32.0	39.0
70-74	35.19536962671338	36.0	34.0	38.0	32.0	39.0
75-79	35.15120170779416	35.6	34.0	37.6	32.0	39.0
80-84	35.10968763282973	35.4	34.0	37.4	32.0	39.0
85-89	35.05629714216528	35.0	34.0	37.0	32.0	39.0
90-94	34.99688018564077	35.0	34.0	37.0	32.0	39.0
95-99	34.92845612051819	35.0	34.0	37.0	31.0	39.0
100-104	34.87894488827884	35.0	34.0	37.0	31.0	39.0
105-109	34.815477787618825	35.0	34.0	37.0	31.0	39.0
110-114	34.73960158417647	35.0	34.0	37.0	31.0	39.0
115-119	34.6685168157543	35.0	34.0	37.0	30.4	39.0
120-124	34.587217718966414	35.0	34.0	37.0	30.0	39.0
125-129	34.50327464372202	35.0	34.0	37.0	30.0	39.0
130-134	34.41511587829114	35.0	34.0	37.0	30.0	39.0
135-139	34.33420653512032	35.0	34.0	37.0	29.8	39.0
140-144	34.19829434835249	35.0	34.0	37.0	29.0	39.0
145-149	34.11508019890273	35.0	34.0	37.0	29.0	39.0
150	34.043984510337246	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	22.0
21	279.0
22	2109.0
23	10357.0
24	33784.0
25	79116.0
26	151985.0
27	252336.0
28	367426.0
29	487582.0
30	632150.0
31	829498.0
32	1117779.0
33	1568522.0
34	2361627.0
35	4045659.0
36	1.0492229E7
37	1651465.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.90251500865743	18.083499900120135	15.912546343655748	32.10143874756668
2	24.267132781012137	25.225717909113932	33.56420232525975	16.94294698461418
3	19.650361923860192	29.175380117524824	30.237735672159527	20.936522286455457
4	23.313870703759225	34.05883083963072	22.30113329081259	20.326165165797462
5	25.085927618217507	35.149371159602545	22.458923133526408	17.305778088653543
6	18.64622775690333	38.969453203511236	22.960639360689136	19.4236796788963
7	20.08726730804308	20.272295226863484	38.44230093149653	21.1981365335969
8	21.428775773458597	22.977629941089774	28.53630675687397	27.05728752857766
9	23.454985529149248	22.980303922951457	28.816912918666564	24.747797629232736
10-14	23.10926458730806	28.97629635491844	25.720464344424144	22.193974713349352
15-19	22.928716003320552	28.135990081022065	27.18819471886091	21.74709919679647
20-24	23.029065892186296	28.206566593587517	27.047449434442456	21.716918079783728
25-29	23.115142364828866	28.204560276762102	26.968242271725302	21.712055086683723
30-34	23.109493785753347	27.975758776608455	27.161945683871487	21.75280175376671
35-39	23.188263504870765	27.969700197801583	27.134706172241106	21.707330125086546
40-44	23.354249490728943	27.741666612342758	27.21986147362227	21.684222423306025
45-49	23.35552253861527	27.58147101638258	27.294679818283836	21.768326626718316
50-54	23.436056921733314	27.58096053149592	27.23310455342244	21.749877993348328
55-59	23.60581056258544	27.463679624087977	27.131203196753624	21.799306616572967
60-64	23.514811305944093	27.449676097338422	27.199784188072606	21.835728408644883
65-69	23.61336414613012	27.37054533410303	27.15206357699135	21.8640269427755
70-74	23.684214700314936	27.346320885380308	27.0651284406081	21.904335973696654
75-79	23.59354097190023	27.385846821613395	27.148685391210815	21.87192681527556
80-84	23.716669532339257	27.430976493747927	27.010887299844256	21.841466674068556
85-89	23.745983681319824	27.357157155353324	27.05205084236034	21.844808320966514
90-94	23.66756874801138	27.299670697472877	27.14405076612694	21.888709788388798
95-99	23.754460702078852	27.405043021835347	27.128456137126772	21.712040138959033
100-104	23.905269325448376	27.37678042350293	26.941905434979457	21.77604481606924
105-109	23.699363388861777	27.511152767282653	27.052140528708495	21.737343315147072
110-114	23.727436876692856	27.50320639075022	26.995821654212378	21.773535078344555
115-119	23.79837877808413	27.495426930476846	27.02074034170416	21.68545394973487
120-124	23.755443099762815	27.410504754352203	27.1988798507287	21.635172295156277
125-129	23.74591661375172	27.491098131614116	27.155662882434218	21.607322372199942
130-134	23.794772224287453	27.42221380536144	27.202957257853193	21.580056712497914
135-139	23.71024118029227	27.431459803512993	27.310306834020835	21.5479921821739
140-144	23.73636066833436	27.56851632164896	27.276687740328793	21.418435269687887
145-149	23.847369115335106	28.081281408647	26.796916433873154	21.274433042144736
150	23.480977961531448	28.022785979349802	26.922338639623355	21.573897419495395
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	3.5
9	6.5
10	6.5
11	5.0
12	7.5
13	11.0
14	17.5
15	38.5
16	65.5
17	126.5
18	203.0
19	331.0
20	497.0
21	784.5
22	1277.0
23	2216.0
24	3741.0
25	5984.5
26	9304.0
27	14887.5
28	25076.5
29	38793.5
30	53037.0
31	70419.5
32	94534.5
33	126992.5
34	172406.5
35	239337.5
36	336834.0
37	458060.0
38	600974.5
39	783366.5
40	999221.5
41	1203236.5
42	1391180.5
43	1569068.5
44	1695747.5
45	1754647.5
46	1757722.5
47	1706729.0
48	1604882.5
49	1458718.5
50	1268457.5
51	1062688.5
52	865526.0
53	687195.5
54	537104.5
55	406953.5
56	303154.0
57	225153.5
58	163468.0
59	117284.5
60	81094.0
61	54091.5
62	36779.5
63	26065.5
64	18445.5
65	13219.5
66	9751.0
67	7208.0
68	6247.5
69	4821.0
70	2722.5
71	1851.0
72	1346.5
73	1055.0
74	944.5
75	551.5
76	166.0
77	65.5
78	18.5
79	0.5
80	0.5
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	8.304291495911404E-7
40-44	0.0
45-49	0.0
50-54	8.304291495911404E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	8.304291495911404E-7
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	8.304291495911404E-7
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4083933E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.141864121911624
#Duplication Level	Percentage of total
1	28.89360650656632
2	10.485155541079228
3	6.972028346076568
4	4.967963465782364
5	4.037781584310171
6	3.1604581774406038
7	2.499576535626576
8	2.157073162625496
9	1.8714782080569827
>10	20.95579600643919
>50	5.516612358803709
>100	6.447444128248396
>500	1.0743400577967375
>1k	0.8597733150693586
>5k	0.10091260607843362
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	2.0760728739778507E-5	0.0	0.0	5.397789472342412E-4	0.0
2	2.0760728739778507E-5	3.3217165983645615E-5	0.0	0.0	0.00426010153740255	0.0
3	4.5673603227512715E-5	3.7369311731601315E-5	0.0	0.0	0.005671831091707488	0.0
4	1.1210793519480394E-4	3.7369311731601315E-5	0.0	4.152145747955702E-6	0.006942387690581933	0.0
5	1.2041222669071534E-4	3.7369311731601315E-5	0.0	4.152145747955702E-6	0.008163118540480909	0.0
6	1.2456437243867104E-4	3.7369311731601315E-5	0.0	4.152145747955702E-6	0.009657891009744962	0.0
7	1.4947724692640526E-4	6.228218621933552E-5	4.152145747955702E-6	4.152145747955702E-6	0.011613551657032097	0.0
8	2.1591157889369646E-4	6.228218621933552E-5	4.152145747955702E-6	4.152145747955702E-6	0.016695778052529874	0.0
9	2.2006372464165218E-4	6.643433196729123E-5	4.152145747955702E-6	4.152145747955702E-6	0.021989763881173394	0.0
10-11	2.6366125499518707E-4	6.643433196729123E-5	4.152145747955702E-6	4.152145747955702E-6	0.027595160640913594	0.0
12-13	3.031066396007662E-4	7.681469633718048E-5	4.152145747955702E-6	4.152145747955702E-6	0.0331714923804181	0.0
14-15	3.3424773271043397E-4	8.719506070706972E-5	4.152145747955702E-6	4.152145747955702E-6	0.040468888532450245	0.0
16-17	3.8199740881192455E-4	8.927113358104757E-5	4.152145747955702E-6	4.152145747955702E-6	0.04571720075786625	0.0
18-19	4.4843174077921575E-4	9.965149795093683E-5	4.152145747955702E-6	4.152145747955702E-6	0.05284228286135823	0.0
20-21	5.127899998725291E-4	1.0172757082491468E-4	4.152145747955702E-6	4.152145747955702E-6	0.05831273488428987	0.0
22-23	5.709200403439089E-4	1.2041222669071534E-4	8.304291495911404E-6	4.152145747955702E-6	0.0640655328180825	0.0
24-25	6.290500808152888E-4	1.2041222669071534E-4	8.304291495911404E-6	4.152145747955702E-6	0.06912907455771447	2.076072873977851E-6
26-27	6.996365585305356E-4	1.2041222669071534E-4	8.304291495911404E-6	4.152145747955702E-6	0.07279334318028538	4.152145747955702E-6
28-29	8.304291495911403E-4	1.2041222669071534E-4	8.304291495911404E-6	4.152145747955702E-6	0.07653650257206746	4.152145747955702E-6
30-31	0.0010193517811231247	1.307925910606046E-4	1.6608582991822807E-5	4.152145747955702E-6	0.08130731803646854	4.152145747955702E-6
32-33	0.0012082744126551092	1.4117295543049385E-4	2.0760728739778507E-5	4.152145747955702E-6	0.08582277653737037	4.152145747955702E-6
34-35	0.001376436315447315	1.4532510117844954E-4	2.0760728739778507E-5	4.152145747955702E-6	0.08863585528161036	4.152145747955702E-6
36-37	0.00142003384580085	1.4740117405242739E-4	2.0760728739778507E-5	4.152145747955702E-6	0.09174166030108122	4.152145747955702E-6
38-39	0.0014636313761543847	1.5362939267436095E-4	2.0760728739778507E-5	4.152145747955702E-6	0.09470006414649966	4.152145747955702E-6
40-41	0.0015238374894997425	1.6193368417027237E-4	2.4912874487734208E-5	4.152145747955702E-6	0.09740933924704076	4.152145747955702E-6
42-43	0.0015591307283573659	1.764661942881173E-4	2.4912874487734208E-5	4.152145747955702E-6	0.10018712475242311	4.152145747955702E-6
44-45	0.0016006521858369228	1.9515085015391797E-4	2.4912874487734208E-5	4.152145747955702E-6	0.10241475094620135	4.152145747955702E-6
46-47	0.001669162590678192	2.2006372464165218E-4	2.4912874487734208E-5	8.304291495911404E-6	0.10489150588485693	4.152145747955702E-6
48-49	0.0017314447768975276	2.2629194326358572E-4	2.4912874487734208E-5	8.304291495911404E-6	0.10741393442674002	6.228218621933553E-6
50-51	0.0017874987444949295	2.2836801613756357E-4	2.4912874487734208E-5	8.304291495911404E-6	0.10937997543839703	8.304291495911404E-6
52-53	0.0018663895137060876	2.2836801613756357E-4	2.4912874487734208E-5	8.304291495911404E-6	0.11135847288729794	1.0380364369889254E-5
54-55	0.0019452802829172462	2.4082445338143068E-4	2.698894736171206E-5	8.304291495911404E-6	0.11317918879777651	1.2456437243867104E-5
56-57	0.0020241710521284044	2.4705267200336424E-4	2.906502023568991E-5	8.304291495911404E-6	0.11518882733978708	1.2456437243867104E-5
58-59	0.002071920728229895	2.5120481775131994E-4	2.906502023568991E-5	1.2456437243867104E-5	0.1169015874608188	1.2456437243867104E-5
60-61	0.0021611918618109427	2.5535696349927563E-4	2.906502023568991E-5	1.2456437243867104E-5	0.118288404140636	1.2456437243867104E-5
62-63	0.002211017610786411	2.6158518212120917E-4	2.906502023568991E-5	1.2456437243867104E-5	0.1202274562049313	1.2456437243867104E-5
64-65	0.0022442347767700566	2.740416193650763E-4	3.114109310966776E-5	1.2456437243867104E-5	0.12183226053651619	1.4532510117844956E-5
66-67	0.0023293537646031483	3.072587853487219E-4	3.3217165983645615E-5	1.4532510117844956E-5	0.1230779042609029	1.6608582991822807E-5
68-69	0.0023708752220827057	3.155630768446333E-4	3.3217165983645615E-5	1.6608582991822807E-5	0.12426126579907028	2.0760728739778507E-5
70-71	0.002391635950822484	3.2386736834054473E-4	3.3217165983645615E-5	1.6608582991822807E-5	0.1254404751914897	2.0760728739778507E-5
72-73	0.0024310813354280632	3.404759513323675E-4	3.3217165983645615E-5	1.6608582991822807E-5	0.1263580994017879	2.4912874487734208E-5
74-75	0.0024539181370418195	3.4255202420634535E-4	3.529323885762346E-5	1.6608582991822807E-5	0.12734008187117943	2.4912874487734208E-5
76-77	0.0024788310115295537	3.550084614502125E-4	4.1521457479557015E-5	1.6608582991822807E-5	0.12850060660773305	2.4912874487734208E-5
78-79	0.002526580687631044	3.736931173160131E-4	4.1521457479557015E-5	1.8684655865800657E-5	0.1300514330445945	2.698894736171206E-5
80-81	0.0025369610520009334	3.840734816859024E-4	5.813004047137982E-5	2.2836801613756358E-5	0.13141333684992396	3.114109310966776E-5
82-83	0.002545265343496845	4.1936672054352584E-4	5.813004047137982E-5	2.4912874487734208E-5	0.1330721190762323	3.3217165983645615E-5
84-85	0.002574330363732535	4.2351886629148154E-4	5.813004047137982E-5	2.4912874487734208E-5	0.13459388049285803	3.529323885762346E-5
86-87	0.0025971671653462914	4.4012744928330436E-4	5.813004047137982E-5	2.906502023568991E-5	0.13635439028999125	3.7369311731601315E-5
88-89	0.0026096236025901585	4.546599594011493E-4	6.228218621933552E-5	2.906502023568991E-5	0.13799241178755978	3.7369311731601315E-5
90-91	0.0026241561127080034	4.6919246951899427E-4	6.228218621933552E-5	3.3217165983645615E-5	0.1388892752691182	3.7369311731601315E-5
92-93	0.002630384331329937	4.712685423929721E-4	6.228218621933552E-5	3.7369311731601315E-5	0.13976953016768484	4.5673603227512715E-5
94-95	0.0026366125499518705	4.7749676101490566E-4	6.228218621933552E-5	3.7369311731601315E-5	0.14064978506625142	4.5673603227512715E-5
96-97	0.002655297205817671	4.837249796368392E-4	6.228218621933552E-5	3.7369311731601315E-5	0.14152173567332213	4.9825748975468415E-5
98-99	0.002659449351565627	5.00333562628662E-4	6.643433196729123E-5	3.7369311731601315E-5	0.14244558810224228	5.605396759740197E-5
100-101	0.002669829715935516	5.024096355026399E-4	7.058647771524692E-5	3.7369311731601315E-5	0.14311615964053714	5.813004047137982E-5
102-103	0.0026739818616834717	5.086378541245734E-4	7.473862346320263E-5	3.7369311731601315E-5	0.14394451271725428	6.643433196729123E-5
104-105	0.0026885143718013167	5.14866072746507E-4	7.473862346320263E-5	3.7369311731601315E-5	0.1447873983040893	6.643433196729123E-5
106-107	0.002721731537784962	5.314746557383298E-4	7.473862346320263E-5	3.7369311731601315E-5	0.14547873057112393	7.058647771524692E-5
108-109	0.0027279597564068957	5.418550201082191E-4	7.473862346320263E-5	3.944538460557917E-5	0.14627801862760537	7.681469633718048E-5
110-111	0.002763252995264519	5.688439674699311E-4	7.473862346320263E-5	4.1521457479557015E-5	0.14710429563144856	8.096684208513618E-5
112-113	0.002775709432508386	5.792243318398203E-4	7.473862346320263E-5	4.1521457479557015E-5	0.14787244259482035	8.304291495911403E-5
114-115	0.0027819376511303198	5.813004047137982E-4	7.473862346320263E-5	4.1521457479557015E-5	0.14878383858649666	8.927113358104757E-5
116-117	0.0027840137240042976	5.813004047137982E-4	7.473862346320263E-5	4.1521457479557015E-5	0.14966616955793724	9.134720645502543E-5
118-119	0.0027881658697522533	5.97908987705621E-4	7.473862346320263E-5	4.359753035353486E-5	0.15038864291808152	9.134720645502543E-5
120-121	0.002792318015500209	6.062132792015324E-4	7.473862346320263E-5	4.5673603227512715E-5	0.15112980093409162	9.549935220298114E-5
122-123	0.0027943940883741873	6.103654249494881E-4	7.473862346320263E-5	4.5673603227512715E-5	0.15177961174364668	9.757542507695898E-5
124-125	0.0028026983798700987	6.186697164453995E-4	7.473862346320263E-5	4.5673603227512715E-5	0.15261211696611182	1.0172757082491468E-4
126-127	0.0028172308899879436	6.228218621933552E-4	7.473862346320263E-5	4.5673603227512715E-5	0.15353804546790595	1.1003186232082609E-4
128-129	0.002837991618727722	6.332022265632444E-4	7.473862346320263E-5	4.5673603227512715E-5	0.15440999607497663	1.1626008094275964E-4
130-131	0.0028483719830976113	6.415065180591559E-4	7.473862346320263E-5	4.5673603227512715E-5	0.155311011702283	1.2041222669071535E-4
132-133	0.002864980566089434	6.435825909331338E-4	7.473862346320263E-5	4.5673603227512715E-5	0.15612483226888232	1.2456437243867104E-4
134-135	0.002891969513451146	6.498108095550673E-4	7.473862346320263E-5	4.5673603227512715E-5	0.15685145777477458	1.2871651818662674E-4
136-137	0.0028961216591991018	6.601911739249565E-4	7.473862346320263E-5	4.5673603227512715E-5	0.1575801593535408	1.2871651818662674E-4
138-139	0.0029106541693169467	6.80951902664735E-4	7.473862346320263E-5	4.5673603227512715E-5	0.15825073089183564	1.39096882556516E-4
>>END_MODULE
