##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.2d.S.1.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24135576
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.866394363242044	37.0	35.0	38.0	33.0	39.0
2	35.47163084071414	35.0	34.0	38.0	33.0	39.0
3	35.60510501178841	35.0	35.0	38.0	33.0	39.0
4	35.675053042032225	36.0	35.0	38.0	33.0	39.0
5	35.59706584172675	36.0	35.0	38.0	33.0	39.0
6	35.54021826535236	35.0	35.0	37.0	33.0	39.0
7	35.57185939958508	35.0	34.0	38.0	33.0	39.0
8	35.67754695392395	36.0	35.0	38.0	33.0	39.0
9	35.65451845856092	36.0	35.0	38.0	33.0	39.0
10-14	35.6370078758427	35.8	35.0	37.8	33.0	39.0
15-19	35.61403554653098	36.0	35.0	38.0	33.0	39.0
20-24	35.60182608444895	36.0	35.0	38.0	33.0	39.0
25-29	35.58406124635268	36.0	35.0	38.0	33.0	39.0
30-34	35.567621008920604	36.0	35.0	38.0	33.0	39.0
35-39	35.538286718328166	36.0	35.0	38.0	33.0	39.0
40-44	35.52398480152287	36.0	35.0	38.0	33.0	39.0
45-49	35.492981820694894	36.0	35.0	38.0	33.0	39.0
50-54	35.47066653805983	36.0	34.6	38.0	33.0	39.0
55-59	35.44608009355153	36.0	34.2	38.0	33.0	39.0
60-64	35.40411953706844	36.0	34.0	38.0	33.0	39.0
65-69	35.37507636030729	36.0	34.0	38.0	32.6	39.0
70-74	35.34203010526867	36.0	34.0	38.0	32.0	39.0
75-79	35.29249625532036	36.0	34.0	38.0	32.0	39.0
80-84	35.25041735071912	36.0	34.0	38.0	32.0	39.0
85-89	35.19786208541284	36.0	34.0	38.0	32.0	39.0
90-94	35.14404794814095	36.0	34.0	38.0	32.0	39.0
95-99	35.074007365724356	36.0	34.0	38.0	32.0	39.0
100-104	35.02508036269778	36.0	34.0	38.0	31.8	39.0
105-109	34.96447592549687	35.4	34.0	37.8	31.2	39.0
110-114	34.88497946765389	35.0	34.0	37.2	31.0	39.0
115-119	34.810211009673026	35.0	34.0	37.0	31.0	39.0
120-124	34.73377246103429	35.0	34.0	37.0	31.0	39.0
125-129	34.650221299876996	35.0	34.0	37.0	30.0	39.0
130-134	34.56157821963727	35.0	34.0	37.0	30.0	39.0
135-139	34.48391108627364	35.0	34.0	37.0	30.0	39.0
140-144	34.35357346350466	35.0	34.0	37.0	29.6	39.0
145-149	34.2740575737658	35.0	34.0	37.0	29.2	39.0
150	34.20685418901956	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	14.0
21	190.0
22	1664.0
23	8131.0
24	26564.0
25	64455.0
26	127841.0
27	222568.0
28	329188.0
29	443707.0
30	581450.0
31	772536.0
32	1052388.0
33	1495464.0
34	2278570.0
35	3927905.0
36	1.0467065E7
37	2335870.0
38	6.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.04089050951177	18.59069367144998	15.113038943010931	32.25537687602732
2	24.621678803107912	25.434594972997537	32.1216116822735	17.822114541621048
3	20.43869182985316	28.975765898439715	29.395391268060063	21.190151003647063
4	23.753574391595212	33.795124674049625	21.373875643158467	21.077425291196697
5	25.94495362364669	34.76556764172523	21.433874211247332	17.855604523380755
6	19.54104596467886	38.98085548072273	21.615879397284736	19.862219157313667
7	20.86865877988576	20.610019831306285	36.58435166411608	21.93696972469188
8	21.934251745224557	23.390139932852648	27.846698168711615	26.828910153211176
9	23.797961150792506	23.115773164062876	27.629943449454036	25.456322235690582
10-14	23.41196663381889	28.639327273564962	25.02433005949392	22.924376033122225
15-19	23.532423672010147	27.597197597438733	26.441381800873533	22.428996929677584
20-24	23.364151740153208	27.653212834033873	26.362417039477325	22.62021838633559
25-29	23.505991321690438	27.297084602414294	26.30546293985277	22.891461136042494
30-34	23.27459763131404	27.037699866785857	26.532057904895247	23.155644597004855
35-39	23.614972550188753	26.81525915781135	26.53814954768968	23.031618744310215
40-44	23.786794232712737	26.489629251027612	26.563796944394447	23.1597795718652
45-49	23.69180601856617	26.34217908321044	26.69423863506915	23.271776263154244
50-54	23.84600475248654	26.32808017509091	26.458686546366245	23.36722852605631
55-59	23.905692575971667	26.29673806003221	26.541474709366785	23.256094654629333
60-64	23.549009975979025	26.264960073876008	26.626234236133417	23.55979571401155
65-69	23.74776822187934	26.19733729327477	26.638578061187168	23.416316423658724
70-74	23.9429496109809	26.190892647434644	26.34074612513909	23.525411616445364
75-79	23.769934473492572	26.28186458031911	26.61768420194322	23.33051674424509
80-84	24.10869249608959	26.243700999719255	26.319421587452478	23.328184916738675
85-89	23.810272437666292	26.375789829917462	26.54659743774087	23.26734029467538
90-94	23.822645044808542	26.123069944549904	26.57461748582259	23.479667524818964
95-99	23.784402742242406	26.37129273401223	26.65279585620828	23.191508667537082
100-104	24.081351114222425	26.080463959095074	26.586952803612395	23.25123212307011
105-109	23.84135269860558	26.293803802320692	26.556989565941997	23.30785393313174
110-114	23.958173610607012	26.364968459837044	26.471272945795864	23.205584983760073
115-119	24.04677642663262	26.42470765976333	26.630544056624128	22.89797185697992
120-124	23.997276054236284	26.59760347132383	26.75770074847188	22.647419725968007
125-129	23.997528992036727	26.63089562586694	26.601860478505756	22.769714903590575
130-134	24.169133764772248	26.627398713230615	26.613380402550828	22.590087119446306
135-139	23.96180807949228	26.68705316997614	26.836891731939605	22.514247018591973
140-144	23.972116513813468	26.85163014133162	26.651499844047642	22.524753500807275
145-149	24.099228458438283	27.518376192886386	26.071423362757116	22.310971985918215
150	23.852614911697156	27.476601345665003	26.013064697523692	22.65771904511415
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.5
9	0.5
10	0.0
11	1.5
12	7.0
13	10.5
14	16.0
15	24.0
16	47.0
17	94.5
18	167.0
19	270.0
20	415.5
21	644.5
22	1013.0
23	1672.0
24	2661.0
25	4324.5
26	6863.0
27	10655.0
28	16297.0
29	23342.0
30	32965.0
31	47120.0
32	67297.5
33	92258.0
34	124350.5
35	175371.5
36	248978.0
37	337128.0
38	447122.5
39	586464.5
40	751417.0
41	935377.5
42	1130717.5
43	1315003.0
44	1461262.0
45	1562930.0
46	1603513.0
47	1582766.0
48	1547464.0
49	1514857.5
50	1419140.0
51	1261884.0
52	1089567.5
53	918455.5
54	769210.0
55	652272.5
56	551075.5
57	461410.5
58	388441.0
59	322301.0
60	250032.5
61	170583.5
62	103242.5
63	61011.0
64	37150.5
65	19057.0
66	10960.5
67	6053.5
68	3127.5
69	1897.5
70	1302.0
71	1187.5
72	1095.5
73	890.5
74	727.5
75	423.0
76	126.5
77	43.0
78	13.0
79	1.5
80	1.0
81	1.0
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	8.286522766226917E-7
40-44	0.0
45-49	8.286522766226917E-7
50-54	0.0
55-59	0.0
60-64	0.0
65-69	8.286522766226917E-7
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	8.286522766226917E-7
130-134	8.286522766226917E-7
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4135576E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.09760763101176
#Duplication Level	Percentage of total
1	25.942890363108965
2	8.23031584818414
3	5.109164022031856
4	3.5266059302669452
5	2.7509611796716036
6	2.1408063011813425
7	1.7395368481551414
8	1.471851497801844
9	1.1894911992808124
>10	15.463446074175154
>50	5.701605706342677
>100	13.504706314534182
>500	5.111166461688737
>1k	6.8352490902404135
>5k	0.735121587406249
>10k+	0.5470815759300288
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.853233086295517E-4	8.286522766226917E-6	0.0	0.0	0.0020799172143229562	0.0
2	0.0018188917471868083	2.9002829681794213E-5	0.0	0.0	0.009483925305946708	0.0
3	0.0027055496831730886	2.9002829681794213E-5	0.0	0.0	0.014070515657053306	0.0
4	0.0063640494844622725	2.9002829681794213E-5	0.0	0.0	0.021192781974625343	0.0
5	0.006562926030851719	2.9002829681794213E-5	0.0	0.0	0.026148122588829037	0.0
6	0.00770232291120792	2.9002829681794213E-5	0.0	0.0	0.033402973270660705	0.0
7	0.009865105353193146	3.314609106490767E-5	8.286522766226917E-6	0.0	0.046445960104701874	0.0
8	0.017198678001303966	3.728935244802113E-5	8.286522766226917E-6	0.0	0.06329246088844119	0.0
9	0.017799450901855418	4.143261383113459E-5	8.286522766226917E-6	0.0	0.07566009611703486	0.0
10-11	0.01866332090023457	4.143261383113459E-5	8.286522766226917E-6	0.0	0.12420047485090059	0.0
12-13	0.01958105329659421	4.9719136597361504E-5	8.286522766226917E-6	0.0	0.14882180562005232	0.0
14-15	0.022858373050636954	5.3862397980474965E-5	8.286522766226917E-6	4.143261383113458E-6	0.15854604008621961	0.0
16-17	0.026460938823254107	5.3862397980474965E-5	8.286522766226917E-6	4.143261383113458E-6	0.16545907170394442	0.0
18-19	0.033218598139112154	6.007729005514515E-5	8.286522766226917E-6	4.143261383113458E-6	0.17195777718335786	0.0
20-21	0.03937134129303564	6.836381282137207E-5	8.286522766226917E-6	4.143261383113458E-6	0.1763558491415328	0.0
22-23	0.04179722083284858	7.872196627915571E-5	8.286522766226917E-6	4.143261383113458E-6	0.18119310680631778	0.0
24-25	0.047388552069360185	8.079359697071244E-5	8.286522766226917E-6	4.143261383113458E-6	0.18576726737327504	0.0
26-27	0.05240604160431059	8.700848904538263E-5	8.286522766226917E-6	4.143261383113458E-6	0.18927660976477212	0.0
28-29	0.06465352225279397	9.115175042849609E-5	8.286522766226917E-6	4.143261383113458E-6	0.19267408409892517	0.0
30-31	0.08873001415006627	9.115175042849609E-5	8.286522766226917E-6	6.214892074670188E-6	0.19615649529143203	0.0
32-33	0.10780973281930376	1.0150990388627974E-4	8.286522766226917E-6	8.286522766226917E-6	0.19900084423093944	0.0
34-35	0.1216150797478378	1.0358153457783648E-4	8.286522766226917E-6	8.286522766226917E-6	0.20200263710300514	0.0
36-37	0.12383793947987817	1.0772479596094993E-4	8.286522766226917E-6	8.286522766226917E-6	0.2039561848451431	4.143261383113458E-6
38-39	0.1260048651832465	1.1186805734406338E-4	8.286522766226917E-6	8.286522766226917E-6	0.20547469014205422	4.143261383113458E-6
40-41	0.12754408678707313	1.1601131872717685E-4	8.286522766226917E-6	8.286522766226917E-6	0.2068626827053972	4.143261383113458E-6
42-43	0.12784240160665733	1.1808294941873358E-4	8.286522766226917E-6	8.286522766226917E-6	0.2083563284340096	4.143261383113458E-6
44-45	0.12869591345157871	1.2844110287651723E-4	8.286522766226917E-6	8.286522766226917E-6	0.20957651891133652	4.143261383113458E-6
46-47	0.13000932731002568	1.2844110287651723E-4	8.286522766226917E-6	8.286522766226917E-6	0.21088371787770882	4.143261383113458E-6
48-49	0.13123366104873568	1.3051273356807396E-4	8.286522766226917E-6	8.286522766226917E-6	0.21196925236008454	4.143261383113458E-6
50-51	0.13212239061541353	1.3258436425963067E-4	8.286522766226917E-6	8.286522766226917E-6	0.21304442868900247	4.143261383113458E-6
52-53	0.13298626061379268	1.3258436425963067E-4	8.286522766226917E-6	8.286522766226917E-6	0.21412996317137822	4.143261383113458E-6
54-55	0.13338608533726315	1.491574097920845E-4	8.286522766226917E-6	8.286522766226917E-6	0.21501454947667292	4.143261383113458E-6
56-57	0.1340676518347853	1.491574097920845E-4	8.286522766226917E-6	8.286522766226917E-6	0.21607108112936688	4.143261383113458E-6
58-59	0.13423545392080138	1.5122904048364125E-4	8.286522766226917E-6	1.0358153457783645E-5	0.21697224048019403	6.214892074670188E-6
60-61	0.13454412689384335	1.5330067117519798E-4	8.286522766226917E-6	1.2429784149340376E-5	0.21763309067080067	8.286522766226917E-6
62-63	0.13473886017884967	1.615871939414249E-4	8.286522766226917E-6	1.2429784149340376E-5	0.21839545076529351	8.286522766226917E-6
64-65	0.13478236442337238	1.6365882463298162E-4	8.286522766226917E-6	1.2429784149340376E-5	0.21908530378558191	8.286522766226917E-6
66-67	0.1348527998668853	1.698737167076518E-4	8.286522766226917E-6	1.2429784149340376E-5	0.21970679299304893	8.286522766226917E-6
68-69	0.13492737857178133	1.8644676224010564E-4	8.286522766226917E-6	1.2429784149340376E-5	0.22039871764402888	1.0358153457783645E-5
70-71	0.13503510336774227	1.8644676224010564E-4	8.286522766226917E-6	1.4501414840897105E-5	0.2211403614316062	1.2429784149340376E-5
72-73	0.13509518065779744	1.8644676224010564E-4	8.286522766226917E-6	1.6573045532453833E-5	0.22164998258172913	1.2429784149340376E-5
74-75	0.1351117537033299	1.8644676224010564E-4	8.286522766226917E-6	2.0716306915567294E-5	0.22228804483472864	1.2429784149340376E-5
76-77	0.13515732957854415	1.9059002362321908E-4	8.286522766226917E-6	2.0716306915567294E-5	0.22316434461725712	1.4501414840897105E-5
78-79	0.13519254730030061	2.0301980777255948E-4	8.286522766226917E-6	2.0716306915567294E-5	0.22422709116202572	1.6573045532453833E-5
80-81	0.13525676785173885	2.0509143846411622E-4	8.286522766226917E-6	2.0716306915567294E-5	0.22491487255162257	1.6573045532453833E-5
82-83	0.13528991394280376	2.1544959192189986E-4	1.2429784149340376E-5	2.0716306915567294E-5	0.22567723264611544	1.6573045532453833E-5
84-85	0.13533134655663492	2.2166448399657003E-4	1.4501414840897105E-5	2.0716306915567294E-5	0.2265783919969426	1.6573045532453833E-5
86-87	0.1353603493863167	2.258077453796835E-4	2.0716306915567294E-5	2.0716306915567294E-5	0.2273925428587244	2.0716306915567294E-5
88-89	0.1353893522159985	2.2787937607124023E-4	2.0716306915567294E-5	2.0716306915567294E-5	0.22818597741359065	2.0716306915567294E-5
90-91	0.13541006852291404	2.3616589883746714E-4	2.0716306915567294E-5	2.0716306915567294E-5	0.22874946096169407	2.4859568298680752E-5
92-93	0.13542871319913807	2.465240522952508E-4	2.0716306915567294E-5	2.0716306915567294E-5	0.22929637146426501	2.4859568298680752E-5
94-95	0.1354701458129692	2.52738944369921E-4	2.0716306915567294E-5	2.0716306915567294E-5	0.23004837340530013	3.107446037335094E-5
96-97	0.13547843233573542	2.52738944369921E-4	2.0716306915567294E-5	2.0716306915567294E-5	0.23075687110181253	3.314609106490767E-5
98-99	0.13550536353472567	2.589538364445912E-4	2.4859568298680752E-5	2.0716306915567294E-5	0.23134728584890618	3.314609106490767E-5
100-101	0.13553850962579056	2.610254671361479E-4	2.4859568298680752E-5	2.0716306915567294E-5	0.23186726515248693	3.314609106490767E-5
102-103	0.1355571543020146	2.630970978277046E-4	2.4859568298680752E-5	2.0716306915567294E-5	0.23237274304122676	3.314609106490767E-5
104-105	0.13558822876238794	2.6516872851926134E-4	2.4859568298680752E-5	2.0716306915567294E-5	0.2329217251744893	3.314609106490767E-5
106-107	0.13562137485345283	2.7138362059393154E-4	2.4859568298680752E-5	2.0716306915567294E-5	0.23341684490977138	3.314609106490767E-5
108-109	0.13564623442175153	2.75526881977045E-4	2.4859568298680752E-5	2.0716306915567294E-5	0.23395132562819299	3.314609106490767E-5
110-111	0.13568766703558266	2.7759851266860174E-4	2.4859568298680752E-5	2.0716306915567294E-5	0.23455831342081912	3.314609106490767E-5
112-113	0.1357063117118067	2.7967014336015847E-4	2.4859568298680752E-5	2.0716306915567294E-5	0.23515908632137056	3.314609106490767E-5
114-115	0.13571666986526446	2.817417740517152E-4	2.4859568298680752E-5	2.0716306915567294E-5	0.2358282230347434	3.314609106490767E-5
116-117	0.1357477443256378	2.817417740517152E-4	2.6931198990237482E-5	2.0716306915567294E-5	0.23632541440071703	3.5217721756464394E-5
118-119	0.13576431737117026	2.900282968179421E-4	2.9002829681794213E-5	2.0716306915567294E-5	0.23682674902807377	3.936098313957786E-5
120-121	0.13577881878601117	2.9417155820105555E-4	2.9002829681794213E-5	2.0716306915567294E-5	0.23756424955426794	4.3504244522691316E-5
122-123	0.1358016067236183	2.9417155820105555E-4	2.9002829681794213E-5	2.0716306915567294E-5	0.2380697274430078	4.557587521424804E-5
124-125	0.1358098932463845	2.9417155820105555E-4	3.314609106490767E-5	2.0716306915567294E-5	0.2386684287128677	4.557587521424804E-5
126-127	0.13582439466122542	2.9417155820105555E-4	3.314609106490767E-5	2.0716306915567294E-5	0.23929613281240936	4.9719136597361504E-5
128-129	0.13582853792260852	2.98314819584169E-4	3.314609106490767E-5	2.0716306915567294E-5	0.23994662484955817	4.9719136597361504E-5
130-131	0.13583682444537476	3.148878651166229E-4	3.314609106490767E-5	2.0716306915567294E-5	0.2407835636489471	4.9719136597361504E-5
132-133	0.1358533974909072	3.190311264997363E-4	3.314609106490767E-5	2.0716306915567294E-5	0.24148791808407638	4.9719136597361504E-5
134-135	0.13588861521266365	3.190311264997363E-4	3.728935244802113E-5	2.0716306915567294E-5	0.2420990491380856	4.9719136597361504E-5
136-137	0.1359155464116539	3.2110275719129304E-4	3.728935244802113E-5	2.0716306915567294E-5	0.24268946388517929	5.3862397980474965E-5
138-139	0.13594040597995258	3.2938927995752E-4	3.728935244802113E-5	2.0716306915567294E-5	0.24329438004711385	5.8005659363588426E-5
>>END_MODULE
