##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.2d.S.2.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24190877
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.873362011637695	36.0	34.0	38.0	30.0	39.0
2	34.7173945781296	35.0	34.0	38.0	31.0	39.0
3	34.944866074925685	35.0	34.0	38.0	31.0	39.0
4	34.87904208681645	36.0	34.0	38.0	31.0	39.0
5	34.742805107892536	35.0	34.0	37.0	31.0	39.0
6	34.72201400552779	35.0	34.0	37.0	31.0	39.0
7	34.75102167647746	35.0	34.0	37.0	32.0	39.0
8	34.883571397597535	35.0	34.0	37.0	31.0	39.0
9	34.867469749029766	35.0	34.0	37.0	31.0	39.0
10-14	34.84912705727866	35.0	34.0	37.4	31.2	39.0
15-19	34.84691065974995	35.0	34.0	37.0	31.0	39.0
20-24	34.85309992688566	35.0	34.0	37.0	31.0	39.0
25-29	34.8472414786781	35.0	34.0	37.0	31.0	39.0
30-34	34.829976755286715	35.0	34.0	37.0	31.0	39.0
35-39	34.823042058375975	35.0	34.0	37.0	31.0	39.0
40-44	34.75701755665989	35.0	34.0	37.0	31.0	39.0
45-49	34.65564329065044	35.0	34.0	37.0	31.0	39.0
50-54	34.619299854238434	35.0	34.0	37.0	30.8	39.0
55-59	34.610229873021964	35.0	34.0	37.0	30.6	39.0
60-64	34.58051007410769	35.0	34.0	37.0	30.0	39.0
65-69	34.563791101910034	35.0	34.0	37.0	30.0	39.0
70-74	34.537352118321294	35.0	34.0	37.0	30.0	39.0
75-79	34.51619167837528	35.0	34.0	37.0	30.0	39.0
80-84	34.48782073506471	35.0	34.0	37.0	30.0	39.0
85-89	34.470542477645594	35.0	34.0	37.0	30.0	39.0
90-94	34.4429597240315	35.0	34.0	37.0	30.0	39.0
95-99	34.408888739337556	35.0	34.0	37.0	30.0	39.0
100-104	34.37049237197973	35.0	34.0	37.0	30.0	39.0
105-109	34.313578329549614	35.0	34.0	37.0	30.0	39.0
110-114	34.28671331758663	35.0	34.0	37.0	30.0	39.0
115-119	34.23609879046551	35.0	34.0	37.0	29.4	39.0
120-124	34.19964993414666	35.0	34.0	37.0	29.0	39.0
125-129	34.145551573016554	35.0	34.0	37.0	29.0	39.0
130-134	34.100805530944584	35.0	34.0	37.0	29.0	39.0
135-139	34.04859889949422	35.0	34.0	37.0	29.0	39.0
140-144	34.02113888636613	35.0	34.0	37.0	29.0	39.0
145-149	33.952321869107934	35.0	34.0	37.0	28.4	39.0
150	33.92555801098075	35.0	34.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	34.0
22	443.0
23	4563.0
24	27261.0
25	99602.0
26	235765.0
27	409560.0
28	587691.0
29	756194.0
30	943224.0
31	1174727.0
32	1492442.0
33	1946275.0
34	2660475.0
35	4075066.0
36	8265668.0
37	1511878.0
38	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.37526400025531	10.71121651210165	13.704764759449164	38.20875472819388
2	22.907794505324247	12.430707231449132	36.301461429443776	28.360036833782843
3	21.55141036159411	16.287420704015688	27.403200705655152	34.75796822873505
4	27.010377345270236	24.057953436893893	21.686715760397032	27.244953457438836
5	27.200509183899857	29.678431947064983	23.750215318863933	19.370843550171227
6	20.229404083386733	36.23699720195871	25.13958272902429	18.39401598563027
7	17.02117157967239	24.279935707858836	39.32736732511738	19.371525387351387
8	19.400957725870448	24.172221005738244	30.773349936858352	25.653471331532955
9	20.164525850250232	22.66528553834551	32.20471096322415	24.965477648180105
10-14	21.208137585693176	28.414551841455634	26.488628704464045	23.888681868387145
15-19	21.428440268982442	27.431516626886136	27.13958349164067	24.000459612490747
20-24	21.407808901374647	27.407656513080013	27.050999117252445	24.133535468292898
25-29	21.337465074637834	27.638743708589992	26.72155375555272	24.302237461219452
30-34	21.35613974936957	27.43777582422452	26.898627644913464	24.30745678149244
35-39	21.59816124287164	27.152922645275734	26.85330156794218	24.395614543910447
40-44	21.800240578933177	27.206906265937462	26.65593546676065	24.33691768836871
45-49	22.031909850436584	27.181687108700764	26.590621013931916	24.19578202693074
50-54	22.00430447214203	27.402742795739453	26.47629437896087	24.11665835315765
55-59	21.959623359300473	27.309657722807046	26.597359046623847	24.133359871268638
60-64	22.17400907344228	27.158331431440995	26.346351481526938	24.321308013589785
65-69	22.045280316952198	27.26236315336113	26.527354049239804	24.165002480446866
70-74	22.398941316358503	26.997236469606044	26.469754860484958	24.1340673535505
75-79	22.223207720206013	26.926548106960063	26.441482196243104	24.408761976590817
80-84	22.487676271266565	26.932877274774857	26.465445251948474	24.114001202010105
85-89	22.34083724125298	26.917385437436796	26.48977395149893	24.25200336981129
90-94	22.319966333624958	26.674070808735017	26.485394822138836	24.52056803550119
95-99	22.570897283418446	26.72662387823606	26.655583668622544	24.046895169722944
100-104	22.532720793229668	26.535221020134443	26.673100086080787	24.258958100555102
105-109	22.658712867626463	26.517006991019993	26.563256795821854	24.261023345531687
110-114	22.56182282203149	26.557220116570022	26.574427473421547	24.30652958797694
115-119	22.68078785645935	26.798930543197564	26.422055392014197	24.09822620832889
120-124	22.582958941438804	26.642811675211682	26.504920571508173	24.26930881184134
125-129	23.00639589041243	26.591313920976557	26.40453748496435	23.99775270364666
130-134	22.671867312431704	26.856592259169815	26.295016932440795	24.176523495957685
135-139	22.814600404928452	26.608452734512987	26.369809526344127	24.207137334214426
140-144	22.753571938918103	26.4767424852297	26.665810750376945	24.10387482547526
145-149	22.595101905889166	26.928102540146035	26.618506708839785	23.858288845125013
150	22.281400794590905	26.47036707695603	26.761692548324028	24.486539580129037
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	1.0
10	1.0
11	2.5
12	10.5
13	16.5
14	23.0
15	58.5
16	102.5
17	228.0
18	539.0
19	968.0
20	1564.0
21	2417.0
22	4061.5
23	6712.0
24	10758.5
25	16237.5
26	22648.5
27	32019.5
28	48653.0
29	72157.0
30	98708.0
31	125069.5
32	152279.0
33	185063.0
34	219694.5
35	264291.5
36	330882.5
37	404718.5
38	476231.5
39	576990.0
40	718239.0
41	875402.0
42	1037036.5
43	1192196.0
44	1329389.5
45	1434786.5
46	1514346.0
47	1546188.0
48	1503300.0
49	1454434.0
50	1380721.5
51	1243081.0
52	1070708.0
53	921078.0
54	780436.5
55	652976.0
56	567344.5
57	474625.0
58	395569.5
59	330659.5
60	261329.0
61	184858.0
62	108048.0
63	60606.0
64	39153.0
65	24901.5
66	16681.5
67	8035.5
68	3491.0
69	2105.5
70	1618.0
71	1395.5
72	1281.5
73	957.5
74	531.5
75	171.5
76	41.0
77	25.0
78	11.5
79	2.5
80	2.5
81	3.0
82	1.0
83	0.0
84	0.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.555059206824126E-4
2	1.6948538079045254E-4
3	9.92109546090454E-5
4	3.3070318203015126E-5
5	4.133789775376891E-5
6	1.0747853415979916E-4
7	2.1082327854422144E-4
8	1.2401369326130673E-4
9	1.1574611371055296E-4
10-14	1.4964318986864346E-4
15-19	2.6290902971397024E-4
20-24	1.7609944443105558E-4
25-29	3.1251450701849296E-4
30-34	8.226241653000013E-4
35-39	7.812862675462324E-4
40-44	9.515984062917603E-4
45-49	8.89591559661107E-4
50-54	5.340856389786944E-4
55-59	4.0428464003185996E-4
60-64	2.3975980697185968E-4
65-69	3.0838071724311605E-4
70-74	3.3483697180552817E-4
75-79	2.538146922081411E-4
80-84	2.7034985130964867E-4
85-89	3.56332678637488E-4
90-94	3.852692070651262E-4
95-99	2.8936528427638236E-4
100-104	4.6546472870743794E-4
105-109	2.9845962178221154E-4
110-114	2.5298793425306573E-4
115-119	2.240514058254275E-4
120-124	2.017289410383923E-4
125-129	1.2814748303668363E-4
130-134	1.4468264213819118E-4
135-139	1.4716291600341732E-4
140-144	1.5625725350924648E-4
145-149	1.9594163535286465E-4
150	2.64562545624121E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4190877E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.94525363789057
#Duplication Level	Percentage of total
1	32.773878768163264
2	8.499888382785283
3	5.046726448234456
4	3.389471878327657
5	2.619662465709802
6	2.1086827496985445
7	1.6747819161563913
8	1.414969378970792
9	1.2382480926001
>10	15.369751895318645
>50	5.17317784667886
>100	11.337513806339599
>500	4.351807548514384
>1k	4.601877482826825
>5k	0.34058619343395335
>10k+	0.05897514624132208
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.6535159101507563E-5	0.0	8.267579550753781E-6	2.27358437645729E-4	2.0668948876884454E-5
2	2.8936528427638237E-5	5.3739267079899587E-5	0.0	1.6535159101507563E-5	0.0022901195355587976	4.5471687529145804E-5
3	5.7873056855276475E-5	6.200684663065336E-5	0.0	1.6535159101507563E-5	0.004489295696059304	5.7873056855276475E-5
4	1.2814748303668363E-4	7.027442618140715E-5	0.0	2.0668948876884454E-5	0.006729809754313578	6.200684663065336E-5
5	1.364150625874374E-4	7.027442618140715E-5	0.0	2.4802738652261346E-5	0.007879003311868355	6.614063640603025E-5
6	1.9842190921809077E-4	7.027442618140715E-5	0.0	2.4802738652261346E-5	0.009660666705055795	6.614063640603025E-5
7	2.4802738652261345E-4	8.68095852829147E-5	0.0	2.4802738652261346E-5	0.011363788092511073	7.440821595678404E-5
8	3.761748695592971E-4	8.68095852829147E-5	0.0	2.4802738652261346E-5	0.015092466469901028	7.854200573216093E-5
9	4.3404792641457354E-4	8.68095852829147E-5	0.0	2.4802738652261346E-5	0.01772569055681611	8.68095852829147E-5
10-11	5.270581963605536E-4	1.0747853415979917E-4	0.0	2.6869633539949793E-5	0.031104701164823417	1.0334474438442227E-4
12-13	5.580616196758803E-4	1.1161232393517606E-4	0.0	3.100342331532668E-5	0.039630642576538255	1.2608058814899518E-4
14-15	6.63473258947991E-4	1.1574611371055295E-4	0.0	3.3070318203015126E-5	0.046325315117761126	1.2814748303668363E-4
16-17	7.709517931077901E-4	1.1987990348592984E-4	0.0	3.3070318203015126E-5	0.05052731242443174	1.2814748303668363E-4
18-19	9.259689096844236E-4	1.2401369326130673E-4	0.0	3.3070318203015126E-5	0.05358424996332295	1.3434816769974895E-4
20-21	0.001345548571885178	1.2814748303668363E-4	0.0	3.3070318203015126E-5	0.056083125882538276	1.364150625874374E-4
22-23	0.0017031213874552792	1.2814748303668363E-4	0.0	3.3070318203015126E-5	0.05877628992119632	1.405488523628143E-4
24-25	0.001837469555155028	1.322812728120605E-4	0.0	3.720410797839202E-5	0.061502524278057386	1.4261574725050276E-4
26-27	0.0019284129302133196	1.322812728120605E-4	0.0	4.133789775376891E-5	0.06364589427659031	1.6121780123969873E-4
28-29	0.0021950423707251293	1.322812728120605E-4	0.0	5.3739267079899587E-5	0.06646307200850965	1.6535159101507563E-4
30-31	0.0026993647233211097	1.322812728120605E-4	0.0	7.027442618140715E-5	0.06941253101324106	1.6948538079045254E-4
32-33	0.0030135327462497536	1.364150625874374E-4	0.0	7.234132106909559E-5	0.07201061788706545	1.8188675011658321E-4
34-35	0.0032388242890077938	1.364150625874374E-4	0.0	8.680958528291472E-5	0.07471618329504962	1.8808743477964854E-4
36-37	0.0032760283969861864	1.364150625874374E-4	0.0	1.0127784949673382E-4	0.07726673158645717	1.9635501433040232E-4
38-39	0.0033070318203015127	1.364150625874374E-4	0.0	1.2814748303668363E-4	0.07978834334943706	2.0668948876884454E-4
40-41	0.003340102138504528	1.364150625874374E-4	0.0	1.2814748303668363E-4	0.08211360009808656	2.1495706831959835E-4
42-43	0.0033628379822691007	1.364150625874374E-4	0.0	1.2814748303668363E-4	0.08455460296044662	2.2322464787035212E-4
44-45	0.0033979751953598044	1.364150625874374E-4	0.0	1.2814748303668363E-4	0.08717749257292326	2.27358437645729E-4
46-47	0.0034496475675520155	1.364150625874374E-4	0.0	1.2814748303668363E-4	0.09113352938795893	2.314922274211059E-4
48-49	0.003474450306204277	1.3848195747512585E-4	0.0	1.322812728120605E-4	0.09375641900043558	2.356260171964828E-4
50-51	0.003513721309070357	1.508833268012565E-4	0.0	1.364150625874374E-4	0.09629663281740466	2.356260171964828E-4
52-53	0.0035653936812625685	1.550171165766334E-4	0.0	1.364150625874374E-4	0.0986012206171773	2.3975980697185968E-4
54-55	0.0036005308943532722	1.5708401146432186E-4	0.0	1.364150625874374E-4	0.10029400753019413	2.3975980697185968E-4
56-57	0.0036253336330055335	1.5708401146432186E-4	0.0	1.4054885236281428E-4	0.10226169146327353	2.4596049163492503E-4
58-59	0.003664604635871614	1.6741848590276409E-4	0.0	1.4881643191356808E-4	0.1042500443452298	2.4802738652261345E-4
60-61	0.00374934732626684	1.6948538079045254E-4	0.0	1.4881643191356808E-4	0.10605237668729414	2.5009428141030193E-4
62-63	0.003809287278009805	1.8188675011658321E-4	0.0	1.550171165766334E-4	0.10827635558644691	2.6042875584874416E-4
64-65	0.0038320231217743783	1.860205398919601E-4	0.0	1.632846961273872E-4	0.110816569403416	2.6042875584874416E-4
66-67	0.003846491385988197	2.08756383656533E-4	0.0	1.6948538079045254E-4	0.11319143162937004	2.6042875584874416E-4
68-69	0.0038754279144158355	2.2942533253341748E-4	0.0	1.6948538079045254E-4	0.11504750323851426	2.6042875584874416E-4
70-71	0.0039271002866080465	2.3975980697185968E-4	0.0	1.736191705658294E-4	0.11704412370002129	2.6662944051180943E-4
72-73	0.003964304394586438	2.4389359674723658E-4	0.0	1.777529603412063E-4	0.11866250239708134	2.728301251748748E-4
74-75	0.003999441607677142	2.4802738652261345E-4	0.0	1.942881194427139E-4	0.12032428588678286	2.8523149450100546E-4
76-77	0.004022177451441715	2.5009428141030193E-4	0.0	1.9842190921809077E-4	0.12166363377400496	2.8936528427638236E-4
78-79	0.004040779505430911	2.5629496607336725E-4	0.0	2.0255569899346764E-4	0.12298437960723789	2.8936528427638236E-4
80-81	0.004075916718521614	2.6662944051180943E-4	0.0	2.0668948876884454E-4	0.12450354734968888	2.9143217916407084E-4
82-83	0.004115187721387695	2.728301251748748E-4	0.0	2.08756383656533E-4	0.1262025349473688	2.9349907405175926E-4
84-85	0.004158592514029153	2.790308098379402E-4	0.0	2.1082327854422144E-4	0.12784364948819343	2.9349907405175926E-4
86-87	0.0042226662555474945	2.810977047256286E-4	0.0	2.1082327854422144E-4	0.1294206902874997	2.9349907405175926E-4
88-89	0.004253669678862821	2.8936528427638236E-4	0.0	2.1495706831959835E-4	0.13091712218618615	2.955659689394477E-4
90-91	0.004282606207290459	2.9349907405175926E-4	0.0	2.1495706831959835E-4	0.13286413717038864	2.9763286382713616E-4
92-93	0.004332211684594982	2.9763286382713616E-4	0.0	2.1495706831959835E-4	0.13469333914599294	2.9763286382713616E-4
94-95	0.004383884056787192	2.9763286382713616E-4	0.0	2.1495706831959835E-4	0.1360905600900703	2.9763286382713616E-4
96-97	0.004460359167631665	3.038335484902015E-4	0.0	2.1702396320728677E-4	0.13736996802554946	2.9763286382713616E-4
98-99	0.004545101858026892	3.183018127040206E-4	0.0	2.27358437645729E-4	0.13847575679046278	2.9763286382713616E-4
100-101	0.00469391828994046	3.2656939225477437E-4	0.0	2.3975980697185968E-4	0.1397634323054927	2.9763286382713616E-4
102-103	0.004716654133705033	3.2656939225477437E-4	0.0	2.3975980697185968E-4	0.1408340838573153	2.9969975871482464E-4
104-105	0.004747657557020359	3.2656939225477437E-4	0.0	2.4182670185954813E-4	0.1419068023040256	3.0176665360251307E-4
106-107	0.0047910623496618164	3.3070318203015127E-4	0.0	2.5216117629799035E-4	0.14300225659450047	3.038335484902015E-4
108-109	0.0048386009320786506	3.3277007691783975E-4	0.0	2.728301251748748E-4	0.1439943661405909	3.100342331532668E-4
110-111	0.004884072619607797	3.3897076158090507E-4	0.0	2.7489702006256323E-4	0.145108422485055	3.100342331532668E-4
112-113	0.004952280150901515	3.43104551356282E-4	0.0	2.8523149450100546E-4	0.14628035188637434	3.100342331532668E-4
114-115	0.004991551153767596	3.472383411316588E-4	0.0	2.8523149450100546E-4	0.1475783618758427	3.100342331532668E-4
116-117	0.00503908973618443	3.472383411316588E-4	0.0	2.8936528427638236E-4	0.14859114037081006	3.1623491781633214E-4
118-119	0.005094895898152018	3.61706605345478E-4	0.0	2.9349907405175926E-4	0.1495088417009437	3.183018127040206E-4
120-121	0.005220976486301014	3.6790729000854333E-4	0.0	2.955659689394477E-4	0.15037900444866054	3.183018127040206E-4
122-123	0.005409063921080662	3.6790729000854333E-4	0.0	3.141680229286437E-4	0.15123469893216357	3.183018127040206E-4
124-125	0.005524810034791215	3.6790729000854333E-4	0.0	3.141680229286437E-4	0.1521379319980834	3.224356024793975E-4
126-127	0.005564081037657296	3.720410797839202E-4	0.0	3.141680229286437E-4	0.1530535664333294	3.224356024793975E-4
128-129	0.0056260878842879485	3.720410797839202E-4	0.0	3.1623491781633214E-4	0.15381625064688642	3.286362871424628E-4
130-131	0.00571703125934624	3.720410797839202E-4	0.0	3.183018127040206E-4	0.1545727341757804	3.3483697180552817E-4
132-133	0.005793506370190713	3.720410797839202E-4	0.0	3.2243560247939747E-4	0.15531681633534825	3.3483697180552817E-4
134-135	0.005884449745249005	3.720410797839202E-4	0.0	3.2656939225477437E-4	0.1561084370773329	3.3483697180552817E-4
136-137	0.0060539351260394565	3.782417644469855E-4	0.0	3.2656939225477437E-4	0.15694552950684673	3.369038666932166E-4
138-139	0.006128343341996241	3.9271002866080463E-4	0.0	3.2656939225477437E-4	0.1577412840386068	3.4103765646859355E-4
>>END_MODULE
