##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.2d.S.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24190877
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.743690317635036	37.0	35.0	38.0	33.0	39.0
2	35.29692734992617	35.0	34.0	37.0	33.0	39.0
3	35.47461458300995	35.0	34.0	37.0	33.0	39.0
4	35.51104980609012	36.0	35.0	38.0	33.0	39.0
5	35.42926405685912	36.0	35.0	37.0	33.0	39.0
6	35.383332485217466	35.0	35.0	37.0	33.0	39.0
7	35.434349527716584	35.0	34.0	38.0	33.0	39.0
8	35.52093675644748	36.0	35.0	38.0	33.0	39.0
9	35.499234401464655	36.0	35.0	38.0	33.0	39.0
10-14	35.48210706871024	35.8	34.6	37.8	33.0	39.0
15-19	35.455787865814045	36.0	34.4	38.0	33.0	39.0
20-24	35.44561045058433	36.0	34.0	38.0	33.0	39.0
25-29	35.427334304581024	36.0	34.0	38.0	33.0	39.0
30-34	35.41254157920774	36.0	34.0	38.0	33.0	39.0
35-39	35.381337088357725	36.0	34.0	38.0	32.8	39.0
40-44	35.36915613270243	36.0	34.0	38.0	32.0	39.0
45-49	35.3379572720741	36.0	34.0	38.0	32.0	39.0
50-54	35.318634004050374	36.0	34.0	38.0	32.0	39.0
55-59	35.28991369763072	36.0	34.0	38.0	32.0	39.0
60-64	35.24819005941786	36.0	34.0	38.0	32.0	39.0
65-69	35.219636286853095	36.0	34.0	38.0	32.0	39.0
70-74	35.1864933875692	36.0	34.0	38.0	32.0	39.0
75-79	35.13488082304747	36.0	34.0	38.0	32.0	39.0
80-84	35.09084698334831	36.0	34.0	38.0	32.0	39.0
85-89	35.04214709536988	35.6	34.0	38.0	32.0	39.0
90-94	34.9887464600808	35.2	34.0	38.0	31.0	39.0
95-99	34.91760345025937	35.0	34.0	37.4	31.0	39.0
100-104	34.868477318949616	35.0	34.0	37.2	31.0	39.0
105-109	34.80804193250208	35.0	34.0	37.0	31.0	39.0
110-114	34.7289207745548	35.0	34.0	37.0	30.8	39.0
115-119	34.65354189515328	35.0	34.0	37.0	30.0	39.0
120-124	34.578678441463694	35.0	34.0	37.0	30.0	39.0
125-129	34.49804611879098	35.0	34.0	37.0	30.0	39.0
130-134	34.39075532482762	35.0	34.0	37.0	29.8	39.0
135-139	34.33328280739884	35.0	34.0	37.0	29.4	39.0
140-144	34.20461520266504	35.0	34.0	37.0	29.0	39.0
145-149	34.12436512326527	35.0	34.0	37.0	28.8	39.0
150	34.056690131573156	35.0	34.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	18.0
21	288.0
22	2448.0
23	11581.0
24	36270.0
25	83636.0
26	160779.0
27	271318.0
28	392654.0
29	514557.0
30	658272.0
31	854166.0
32	1135749.0
33	1577176.0
34	2342163.0
35	3922008.0
36	9949769.0
37	2278018.0
38	6.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.37848904775135	19.018950821832544	13.983296264951454	32.61926386546465
2	23.746861265095927	27.059519173281732	32.10764950770491	17.085970053917436
3	19.63583627001204	30.112686695897793	28.70709896131504	21.54437807277512
4	23.105280556798334	34.716695058223806	21.42300587118028	20.755018513797577
5	24.949273232218907	35.29074204296107	21.759169789503705	18.000814935316317
6	18.950408453567018	39.28482625908932	22.05485977213641	19.70990551520724
7	20.286622928139398	20.966230368580685	36.53359487545656	22.21355182782336
8	21.536077422906164	23.974649616878295	27.850209812566945	26.639063147648596
9	23.454643665874535	23.816490820072378	27.609151995605618	25.119713518447472
10-14	23.08664129870116	29.028028210800294	25.01038552674217	22.874944963756377
15-19	23.136556810238833	27.951743130271794	26.574770315272154	22.33692974421721
20-24	23.02763889047925	27.853687156525993	26.52769471731017	22.590979235684593
25-29	23.113930925282286	27.431049316649414	26.50888928086402	22.946130477204278
30-34	23.016559507123286	27.03938513680178	26.742339271122745	23.20171608495219
35-39	23.319171107355885	26.729637788658923	26.785708513172136	23.165482590813056
40-44	23.516332210692486	26.300154392914322	26.82678846244392	23.356724933949273
45-49	23.411864922564526	26.085105770122397	26.953990357224257	23.54903895008882
50-54	23.636698247856	26.10755782024769	26.65068819125491	23.6050557406414
55-59	23.65777230813087	26.082712090181765	26.691977310289328	23.567538291398034
60-64	23.351820605759766	26.04334435663494	26.78192030822198	23.822914729383314
65-69	23.573204258557507	25.96915461948035	26.744713114325812	23.712928007636332
70-74	23.798148368081073	25.93368070119988	26.45264163014842	23.815529300570624
75-79	23.626946637775887	26.047593065766073	26.706126445932487	23.61933385052555
80-84	23.95449325793356	26.032104582235693	26.390780292917864	23.622621866912887
85-89	23.662192982916658	26.17192588759804	26.55808799325465	23.607793136230654
90-94	23.650923445231026	25.92020123950033	26.60612593747635	23.8227493777923
95-99	23.70012687923948	26.089481404650556	26.665959898831943	23.54443181727802
100-104	23.945854464060975	25.843152358635034	26.611220419995522	23.599772757308468
105-109	23.734344149656085	26.07945797087059	26.489644009185774	23.696553870287545
110-114	23.842598182777746	26.191039704761426	26.410091705232514	23.55627040722831
115-119	23.996050246545423	26.26473277508707	26.559610881407895	23.17960609695961
120-124	23.940869940349828	26.429474218731304	26.706538998152073	22.9231168427668
125-129	23.938210853741538	26.49721942277379	26.49838101772948	23.0661887057552
130-134	24.107155493745598	26.54223991983532	26.4586702476143	22.891934338804784
135-139	23.896385401819042	26.573209396252977	26.71845175352675	22.81195344840123
140-144	23.92296318980085	26.7278916758578	26.541459410504213	22.80768572383713
145-149	24.050848590565774	27.350831472542314	25.99688965389721	22.6014302829947
150	23.870407840112616	27.287427404967584	25.944838626561573	22.897326128358223
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.5
9	2.0
10	2.0
11	6.0
12	9.0
13	9.0
14	20.5
15	34.0
16	49.0
17	103.0
18	198.5
19	333.0
20	488.5
21	757.5
22	1217.0
23	1896.0
24	2989.5
25	4780.5
26	7495.5
27	11518.0
28	17122.0
29	24154.0
30	34248.0
31	49227.0
32	70516.0
33	96845.5
34	130545.5
35	184831.0
36	262333.0
37	352484.0
38	463213.5
39	602850.5
40	770439.5
41	952722.0
42	1143186.5
43	1330111.0
44	1470454.5
45	1569431.0
46	1612551.5
47	1579182.0
48	1530359.0
49	1491117.5
50	1398609.5
51	1244834.5
52	1076413.5
53	919077.5
54	772422.5
55	656072.0
56	553816.0
57	450157.5
58	374743.5
59	312263.5
60	241046.0
61	167274.0
62	105246.0
63	63798.0
64	38607.0
65	19234.0
66	10858.0
67	6009.5
68	3179.0
69	1985.0
70	1298.0
71	1104.0
72	956.5
73	787.0
74	668.0
75	398.5
76	121.5
77	47.0
78	12.5
79	1.0
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	8.267579550753782E-7
50-54	0.0
55-59	0.0
60-64	0.0
65-69	8.267579550753782E-7
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	2.4802738652261347E-6
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	2.4802738652261347E-6
130-134	0.050635617716546615
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4190877E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.08373541050116
#Duplication Level	Percentage of total
1	24.9023143057016
2	8.239508715416184
3	5.128614819277299
4	3.5201031330271735
5	2.611937576208403
6	2.0804609281491206
7	1.8107133914365687
8	1.4927401355293903
9	1.2652972579426622
>10	16.006693787134576
>50	5.779140543449747
>100	13.415586108056154
>500	4.687936174350753
>1k	6.772658342925281
>5k	1.2008215119391448
>10k+	1.085473269455902
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	27803	0.11493175712480372	No Hit
CTTAAACGTTTTACTTTGTGCTGCACTACTCAACCTCAATGGCCGCCTCA	24562	0.10153414446280719	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.555059206824126E-4	4.133789775376891E-6	0.0	0.0	0.002037958359260807	0.0
2	0.0018850081375718624	3.3070318203015126E-5	4.133789775376891E-6	0.0	0.011677956115439717	0.0
3	0.0027944418881547784	3.3070318203015126E-5	4.133789775376891E-6	0.0	0.016927869130168367	0.0
4	0.007105984623872875	3.3070318203015126E-5	4.133789775376891E-6	0.0	0.025013561930805568	0.0
5	0.0074284202263522735	3.3070318203015126E-5	4.133789775376891E-6	0.0	0.030565241599136733	0.0
6	0.008904183176161823	3.3070318203015126E-5	4.133789775376891E-6	0.0	0.040800505082969916	0.0
7	0.011777167070048762	4.133789775376891E-5	4.133789775376891E-6	0.0	0.05670319434884481	0.0
8	0.022264591730179934	4.133789775376891E-5	4.133789775376891E-6	0.0	0.07594598575322424	0.0
9	0.02304174420795079	4.133789775376891E-5	4.133789775376891E-6	4.133789775376891E-6	0.08909970481847351	4.133789775376891E-6
10-11	0.024058656492693505	4.133789775376891E-5	4.133789775376891E-6	4.133789775376891E-6	0.1507799820568721	4.133789775376891E-6
12-13	0.02516031146783145	4.133789775376891E-5	4.133789775376891E-6	4.133789775376891E-6	0.1827300432307601	4.133789775376891E-6
14-15	0.02944291767512191	4.960547730452269E-5	4.133789775376891E-6	4.133789775376891E-6	0.19697301590182115	4.133789775376891E-6
16-17	0.03434765924360658	5.3739267079899587E-5	4.133789775376891E-6	4.133789775376891E-6	0.20625337394754228	4.133789775376891E-6
18-19	0.043059621195213386	5.993995174296492E-5	4.133789775376891E-6	4.133789775376891E-6	0.21488472699852923	4.133789775376891E-6
20-21	0.04989277569391139	7.234132106909559E-5	4.133789775376891E-6	4.133789775376891E-6	0.22007470006151492	4.133789775376891E-6
22-23	0.052941445653251845	7.854200573216093E-5	1.2401369326130671E-5	4.133789775376891E-6	0.22532874686601895	4.133789775376891E-6
24-25	0.059611315455822454	7.854200573216093E-5	1.6535159101507563E-5	4.133789775376891E-6	0.23057452609097223	4.133789775376891E-6
26-27	0.06548956451640839	8.060890061984937E-5	1.6535159101507563E-5	4.133789775376891E-6	0.2350700224716946	4.133789775376891E-6
28-29	0.0795113794344868	8.267579550753782E-5	1.6535159101507563E-5	4.133789775376891E-6	0.23916247434931773	4.133789775376891E-6
30-31	0.10881168136235822	8.68095852829147E-5	2.0668948876884454E-5	4.133789775376891E-6	0.24416022618774838	4.133789775376891E-6
32-33	0.1341270099467663	9.301026994598004E-5	2.0668948876884454E-5	4.133789775376891E-6	0.2479323093577798	4.133789775376891E-6
34-35	0.15239629385904446	9.50771648336685E-5	2.0668948876884454E-5	4.133789775376891E-6	0.2522190493548456	4.133789775376891E-6
36-37	0.1555586430372078	9.50771648336685E-5	2.0668948876884454E-5	4.133789775376891E-6	0.2547096576945102	4.133789775376891E-6
38-39	0.15857217578345753	9.50771648336685E-5	2.0668948876884454E-5	4.133789775376891E-6	0.25652645830078835	4.133789775376891E-6
40-41	0.1602649626964744	9.50771648336685E-5	2.8936528427638237E-5	4.133789775376891E-6	0.2582688506911097	4.133789775376891E-6
42-43	0.1606328699864829	1.0127784949673383E-4	2.8936528427638237E-5	4.133789775376891E-6	0.25996990518367735	4.133789775376891E-6
44-45	0.16161464505813494	1.1161232393517606E-4	2.8936528427638237E-5	4.133789775376891E-6	0.261458069502813	4.133789775376891E-6
46-47	0.16311521074659674	1.1987990348592984E-4	2.8936528427638237E-5	4.133789775376891E-6	0.2629669027708256	4.133789775376891E-6
48-49	0.1645765054321925	1.2194679837361827E-4	2.8936528427638237E-5	6.200684663065336E-6	0.2643248527120369	8.267579550753781E-6
50-51	0.16572776588463495	1.2401369326130673E-4	2.8936528427638237E-5	8.267579550753781E-6	0.26561872891172983	8.267579550753781E-6
52-53	0.16697823729168645	1.2608058814899518E-4	2.8936528427638237E-5	8.267579550753781E-6	0.2669560099040642	1.0334474438442227E-5
54-55	0.16755283407046384	1.2814748303668363E-4	2.8936528427638237E-5	8.267579550753781E-6	0.2681403406747097	1.2401369326130673E-5
56-57	0.16836719065621308	1.3434816769974895E-4	2.8936528427638237E-5	8.267579550753781E-6	0.26930606939136603	1.2401369326130673E-5
58-59	0.16855734498588043	1.3848195747512585E-4	2.8936528427638237E-5	8.267579550753781E-6	0.2702609748294781	1.2401369326130673E-5
60-61	0.16892938606566432	1.4261574725050276E-4	2.8936528427638237E-5	1.0334474438442227E-5	0.271011257673709	1.6535159101507563E-5
62-63	0.16930349404033596	1.4468264213819118E-4	3.100342331532668E-5	1.4468264213819119E-5	0.2718814204214258	1.6535159101507563E-5
64-65	0.16934483193808972	1.4468264213819118E-4	3.3070318203015126E-5	1.6535159101507563E-5	0.27274124869470423	1.6535159101507563E-5
66-67	0.16943164152337264	1.4674953702587963E-4	3.3070318203015126E-5	1.6535159101507563E-5	0.27345019364118134	1.6535159101507563E-5
68-69	0.1694895145802279	1.4881643191356808E-4	3.3070318203015126E-5	1.6535159101507563E-5	0.27421081095985067	1.6535159101507563E-5
70-71	0.16961972895815228	1.4881643191356808E-4	3.3070318203015126E-5	1.6535159101507563E-5	0.27501896686093685	1.6535159101507563E-5
72-73	0.16968793648944602	1.508833268012565E-4	3.3070318203015126E-5	1.6535159101507563E-5	0.2755170885288698	1.6535159101507563E-5
74-75	0.16972514059742438	1.5295022168894496E-4	3.3070318203015126E-5	1.6535159101507563E-5	0.27637691680214815	2.27358437645729E-5
76-77	0.16978508054916736	1.5708401146432186E-4	3.720410797839202E-5	1.6535159101507563E-5	0.2772987519220572	2.4802738652261346E-5
78-79	0.16984088671113495	1.6121780123969876E-4	3.720410797839202E-5	1.6535159101507563E-5	0.27841280826652126	2.4802738652261346E-5
80-81	0.16989049218843946	1.6948538079045254E-4	3.9271002866080465E-5	1.860205398919601E-5	0.27932844270176727	2.4802738652261346E-5
82-83	0.16993803077085629	1.6948538079045254E-4	4.133789775376891E-5	2.0668948876884454E-5	0.28009112691532434	2.4802738652261346E-5
84-85	0.1699917700379362	1.736191705658294E-4	4.5471687529145804E-5	2.27358437645729E-5	0.2809344200295012	2.4802738652261346E-5
86-87	0.17003724172546536	1.736191705658294E-4	4.5471687529145804E-5	2.6869633539949793E-5	0.28182525172609496	2.6869633539949793E-5
88-89	0.17005997756922991	1.777529603412063E-4	4.753858241683425E-5	2.8936528427638237E-5	0.28271194963291324	2.8936528427638237E-5
90-91	0.17007237893855606	1.8808743477964854E-4	4.960547730452269E-5	2.8936528427638237E-5	0.2833650884174228	2.8936528427638237E-5
92-93	0.17007651272833144	1.90154329667337E-4	4.960547730452269E-5	2.8936528427638237E-5	0.2838900797188957	2.8936528427638237E-5
94-95	0.1701343857851867	1.9222122455502544E-4	4.960547730452269E-5	3.3070318203015126E-5	0.284491546131213	2.8936528427638237E-5
96-97	0.17014678715451284	1.9635501433040232E-4	4.960547730452269E-5	3.720410797839202E-5	0.2851033470179688	2.8936528427638237E-5
98-99	0.17016125541872668	2.0048880410577922E-4	4.960547730452269E-5	3.720410797839202E-5	0.2857647533820291	2.8936528427638237E-5
100-101	0.1701901919471543	2.0255569899346767E-4	4.960547730452269E-5	4.133789775376891E-5	0.28628354399883893	2.8936528427638237E-5
102-103	0.17021706158069425	2.0255569899346767E-4	5.3739267079899587E-5	4.133789775376891E-5	0.286800267720761	2.8936528427638237E-5
104-105	0.17024806500400957	2.0255569899346767E-4	5.3739267079899587E-5	4.133789775376891E-5	0.2874079348177414	2.8936528427638237E-5
106-107	0.17028526911198796	2.0255569899346767E-4	5.3739267079899587E-5	4.133789775376891E-5	0.2879494612783158	2.8936528427638237E-5
108-109	0.17032247321996635	2.08756383656533E-4	5.3739267079899587E-5	4.133789775376891E-5	0.2884992553184409	2.8936528427638237E-5
110-111	0.17037414559215858	2.1082327854422144E-4	5.3739267079899587E-5	4.133789775376891E-5	0.28901184525058765	2.8936528427638237E-5
112-113	0.1704010152256985	2.1082327854422144E-4	5.3739267079899587E-5	4.133789775376891E-5	0.2896381144015573	2.8936528427638237E-5
114-115	0.1704196172796877	2.128901734319099E-4	5.3739267079899587E-5	4.133789775376891E-5	0.2902209787598854	2.8936528427638237E-5
116-117	0.17044442001833998	2.1495706831959835E-4	5.3739267079899587E-5	4.133789775376891E-5	0.29082451206709037	2.8936528427638237E-5
118-119	0.17046302207232916	2.1495706831959835E-4	5.3739267079899587E-5	4.133789775376891E-5	0.29143011226918314	2.8936528427638237E-5
120-121	0.1704733565467676	2.1495706831959835E-4	5.3739267079899587E-5	4.133789775376891E-5	0.29197783941442057	3.100342331532668E-5
122-123	0.17049609239053218	2.1495706831959835E-4	5.3739267079899587E-5	4.133789775376891E-5	0.292492496241455	3.3070318203015126E-5
124-125	0.17051676133940907	2.1495706831959835E-4	5.3739267079899587E-5	4.133789775376891E-5	0.2931208322873123	3.3070318203015126E-5
126-127	0.17054363097294903	2.1909085809497522E-4	5.3739267079899587E-5	4.133789775376891E-5	0.2937284993842927	3.720410797839202E-5
128-129	0.17055396544738746	2.2322464787035212E-4	5.3739267079899587E-5	4.133789775376891E-5	0.29435063474548695	3.720410797839202E-5
130-131	0.1705622330269382	2.314922274211059E-4	5.3739267079899587E-5	4.133789775376891E-5	0.2951133189590439	3.9271002866080465E-5
132-133	0.17057876818603973	2.3975980697185968E-4	5.7873056855276475E-5	4.133789775376891E-5	0.29575819016400273	4.133789775376891E-5
134-135	0.17060977160935506	2.5216117629799035E-4	6.200684663065336E-5	4.340479264145736E-5	0.2962728469910372	4.133789775376891E-5
136-137	0.17061390539913043	2.5216117629799035E-4	6.407374151834181E-5	4.5471687529145804E-5	0.2968329755056007	4.340479264145736E-5
138-139	0.17063044055823193	2.624956507364326E-4	6.614063640603025E-5	4.5471687529145804E-5	0.2974261743383673	4.5471687529145804E-5
>>END_MODULE
