##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.2d.S.3.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24083958
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.81245038709999	37.0	35.0	38.0	33.0	39.0
2	35.413746486354114	35.0	34.0	38.0	33.0	39.0
3	35.531649822674495	35.0	35.0	37.0	33.0	39.0
4	35.61864773223737	36.0	35.0	38.0	33.0	39.0
5	35.5410099535965	36.0	35.0	38.0	33.0	39.0
6	35.48625500011252	35.0	35.0	37.0	33.0	39.0
7	35.51916744747687	35.0	34.0	38.0	33.0	39.0
8	35.62659850179111	36.0	35.0	38.0	33.0	39.0
9	35.609981797842366	36.0	35.0	38.0	33.0	39.0
10-14	35.581724407591146	35.8	35.0	37.8	33.0	39.0
15-19	35.56205024938176	36.0	35.0	38.0	33.0	39.0
20-24	35.54800028300996	36.0	35.0	38.0	33.0	39.0
25-29	35.53359435355269	36.0	35.0	38.0	33.0	39.0
30-34	35.51599116723256	36.0	35.0	38.0	33.0	39.0
35-39	35.490041645148196	36.0	35.0	38.0	33.0	39.0
40-44	35.47461039418853	36.0	34.8	38.0	33.0	39.0
45-49	35.44823953770389	36.0	34.0	38.0	33.0	39.0
50-54	35.427238894869355	36.0	34.2	38.0	33.0	39.0
55-59	35.404315112989316	36.0	34.0	38.0	32.8	39.0
60-64	35.36288985390192	36.0	34.0	38.0	32.0	39.0
65-69	35.33812982899239	36.0	34.0	38.0	32.0	39.0
70-74	35.304556800838135	36.0	34.0	38.0	32.0	39.0
75-79	35.255511266046895	36.0	34.0	38.0	32.0	39.0
80-84	35.216765691087815	36.0	34.0	38.0	32.0	39.0
85-89	35.16848362715132	36.0	34.0	38.0	32.0	39.0
90-94	35.11718775626498	36.0	34.0	38.0	32.0	39.0
95-99	35.050978921321814	35.8	34.0	38.0	32.0	39.0
100-104	35.00555772435743	36.0	34.0	38.0	31.0	39.0
105-109	34.94743819931923	35.4	34.0	38.0	31.0	39.0
110-114	34.87141843545815	35.0	34.0	37.8	31.0	39.0
115-119	34.79810226375582	35.0	34.0	37.0	31.0	39.0
120-124	34.723840392015305	35.0	34.0	37.0	30.4	39.0
125-129	34.64780764856009	35.0	34.0	37.0	30.0	39.0
130-134	34.560779204149085	35.0	34.0	37.0	30.0	39.0
135-139	34.49027970402539	35.0	34.0	37.0	30.0	39.0
140-144	34.365918666690916	35.0	34.0	37.0	29.6	39.0
145-149	34.28952604052872	35.0	34.0	37.0	29.0	39.0
150	34.22573274708418	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	20.0
21	259.0
22	1918.0
23	9517.0
24	30872.0
25	72854.0
26	141813.0
27	244823.0
28	359373.0
29	470954.0
30	603405.0
31	784753.0
32	1052703.0
33	1473727.0
34	2220547.0
35	3788847.0
36	1.0309281E7
37	2518283.0
38	8.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.589088637341085	18.850352587394482	15.343495450374062	32.21706332489037
2	24.592735130994665	25.682672258438583	32.36112187207767	17.36347073848908
3	19.82393010318321	29.450503941254176	30.010760689750416	20.714805265812206
4	23.422512196707867	34.28933068227407	21.46549582921545	20.822661291802618
5	25.579674237930494	35.21963457999719	21.402125846590497	17.79856533548182
6	18.992276933882714	39.50826521122483	21.501428461218875	19.99802939367358
7	20.453959436401608	20.765822627659457	36.83999531970617	21.940222616232763
8	21.45725382846125	23.600464674452596	27.904234013362757	27.0380474837234
9	23.675614282336817	23.57507432955995	27.354112642116384	25.395198745986853
10-14	23.082999895615163	29.086476566683935	25.01667458480039	22.813848952900514
15-19	23.233734255806294	27.842333888806813	26.555113573939966	22.368818281446927
20-24	23.108065543047367	27.782464161414	26.522313317437273	22.58715697810136
25-29	23.203231794375327	27.333875104748145	26.51428805846614	22.94860504241039
30-34	23.049889889361207	26.99896254594033	26.761800531291406	23.189347033407053
35-39	23.36785091553473	26.701264800411963	26.783596782555424	23.147287501497885
40-44	23.571191246887242	26.337022344915233	26.81273983287963	23.279046575317892
45-49	23.436006656380982	26.123261799410212	26.93528696570555	23.50544457850325
50-54	23.681309359533014	26.132340871878284	26.634127995074564	23.552221773514137
55-59	23.715345293327616	26.133897094489207	26.68304935592397	23.467708256259208
60-64	23.39098166505688	26.082164733886348	26.76791580520112	23.758937795855648
65-69	23.60087739731152	26.02198027417254	26.733338432163016	23.643803896352917
70-74	23.810418536687365	26.00581930926802	26.442116366421164	23.741645787623447
75-79	23.64413523723966	26.11202444382273	26.712239740660564	23.531600578277043
80-84	23.980635574933324	26.09722205959668	26.400959510060595	23.521182855409396
85-89	23.66221947405821	26.22460560676945	26.599111325472336	23.514063593700005
90-94	23.68253756297034	25.963465805745052	26.63495759293385	23.719039038350758
95-99	23.69707919271409	26.158353207558328	26.695244195327028	23.449323404400555
100-104	23.9465008201725	25.901652045731023	26.653171376565265	23.498675757531217
105-109	23.75092250202396	26.153927855213833	26.498466738731235	23.596682904030974
110-114	23.869727724986067	26.261430118753736	26.413050545927707	23.45579161033249
115-119	23.98251171159252	26.327020885246693	26.59410821588468	23.096359187276107
120-124	23.957109541546288	26.487397960086128	26.714717738670696	22.84077475969689
125-129	23.927415484239184	26.56765099104808	26.51913570882553	22.985797815887203
130-134	24.123168625356346	26.60142988125125	26.47627935574377	22.799122137648638
135-139	23.865457030488567	26.636492445606265	26.75097529028223	22.747075233622937
140-144	23.93088212493976	26.800770039542503	26.53673038293789	22.731617452579847
145-149	24.044630870058818	27.422759996508876	26.00528534387911	22.527323789553197
150	23.875701826086893	27.36521546832128	25.95008262346247	22.80900008212936
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	3.5
10	4.5
11	3.5
12	6.5
13	10.0
14	12.5
15	23.0
16	44.5
17	84.0
18	174.5
19	308.0
20	464.5
21	694.0
22	1083.0
23	1693.5
24	2702.5
25	4389.5
26	6887.5
27	10690.5
28	16161.5
29	23071.0
30	32928.0
31	47522.5
32	68837.5
33	95203.5
34	129005.5
35	184133.5
36	261844.5
37	351786.5
38	461957.0
39	603259.0
40	773382.0
41	953972.0
42	1144136.0
43	1328072.0
44	1466886.5
45	1563765.5
46	1599604.5
47	1571052.0
48	1528724.0
49	1485904.5
50	1389149.0
51	1237599.5
52	1071564.0
53	909655.5
54	767532.5
55	654546.0
56	548833.5
57	447992.0
58	372839.0
59	310403.5
60	239344.0
61	164472.5
62	102930.5
63	62395.5
64	37998.5
65	19181.0
66	11022.5
67	6065.0
68	3162.0
69	1958.0
70	1210.0
71	991.5
72	849.0
73	698.5
74	576.0
75	339.0
76	109.5
77	35.5
78	9.5
79	3.0
80	1.0
81	0.5
82	0.5
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	8.304282875763195E-7
120-124	0.0
125-129	1.660856575152639E-6
130-134	0.0
135-139	8.304282875763195E-7
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4083958E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.450485240697546
#Duplication Level	Percentage of total
1	25.284067495160944
2	8.227622721576559
3	4.988039698756939
4	3.5578015998569557
5	2.7154112135963953
6	2.2109742574346876
7	1.7779778587645203
8	1.4467010446156745
9	1.284138582693186
>10	15.968037243162819
>50	5.879324208086675
>100	13.236245999028162
>500	4.750754361241561
>1k	6.730441683812699
>5k	1.160596642406044
>10k+	0.7818653898061754
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	32206	0.13372386714841472	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.2767056810180453E-4	0.0	0.0	0.0	0.002084375001816562	0.0
2	0.0020968314261302066	4.152141437881598E-6	0.0	0.0	0.010384505736141875	0.0
3	0.0030061504010262764	4.152141437881598E-6	0.0	0.0	0.014669515700035683	0.0
4	0.007706374508708245	8.304282875763195E-6	0.0	0.0	0.021503940506788794	0.0
5	0.007984567985046312	1.2456424313644791E-5	0.0	0.0	0.026166795341529825	0.0
6	0.009130559021901633	1.2456424313644791E-5	0.0	0.0	0.03429253613546411	0.0
7	0.011920798068158066	2.0760707189407987E-5	0.0	0.0	0.04686937255080747	0.0
8	0.020702577209277644	2.0760707189407987E-5	0.0	0.0	0.06516785986755166	0.0
9	0.02144165838522057	2.0760707189407987E-5	0.0	0.0	0.07810178044655285	0.0
10-11	0.022135066005346794	2.4912848627289583E-5	0.0	0.0	0.13203186951247797	0.0
12-13	0.022988331070831466	2.4912848627289583E-5	0.0	0.0	0.15842080442093448	0.0
14-15	0.02670449765773549	3.321713150305278E-5	0.0	0.0	0.1692620457152433	0.0
16-17	0.031176353986333977	3.321713150305278E-5	0.0	0.0	0.17730681975113893	0.0
18-19	0.03978581925778146	3.9445343659875174E-5	0.0	0.0	0.1852581706046822	0.0
20-21	0.047203619936556945	4.5673555816697566E-5	0.0	0.0	0.19040059777549853	0.0
22-23	0.0500561411043816	5.1901767973519965E-5	0.0	0.0	0.19550150353193607	0.0
24-25	0.05686772913322635	5.6053909411401565E-5	0.0	4.152141437881598E-6	0.2004944536109887	0.0
26-27	0.06307518058285935	5.8129980130342364E-5	0.0	4.152141437881598E-6	0.20445767261344666	0.0
28-29	0.07787341266746936	5.8129980130342364E-5	0.0	4.152141437881598E-6	0.20788526537041793	0.0
30-31	0.10853697718622496	6.228212156822396E-5	2.076070718940799E-6	4.152141437881598E-6	0.21185056044359485	0.0
32-33	0.13191976169365516	6.228212156822396E-5	4.152141437881598E-6	4.152141437881598E-6	0.2149459818855356	0.0
34-35	0.14881482520439537	6.228212156822396E-5	4.152141437881598E-6	4.152141437881598E-6	0.21843378069335612	0.0
36-37	0.1515469342705215	6.228212156822396E-5	4.152141437881598E-6	4.152141437881598E-6	0.22057213353386518	0.0
38-39	0.15384722062710787	6.435819228716477E-5	4.152141437881598E-6	4.152141437881598E-6	0.22223091403829887	0.0
40-41	0.1554395668685355	6.851033372504636E-5	4.152141437881598E-6	4.152141437881598E-6	0.22395612880573867	0.0
42-43	0.15574682533493872	7.889068731975035E-5	4.152141437881598E-6	4.152141437881598E-6	0.22555885540076095	0.0
44-45	0.1566623725219916	8.927104091445434E-5	4.152141437881598E-6	4.152141437881598E-6	0.22682525853931484	0.0
46-47	0.15822772984407296	9.549925307127675E-5	4.152141437881598E-6	4.152141437881598E-6	0.2281913130723779	2.076070718940799E-6
48-49	0.15954395867988144	9.965139450915833E-5	4.152141437881598E-6	4.152141437881598E-6	0.22947640084740223	4.152141437881598E-6
50-51	0.16074600362614816	9.965139450915833E-5	4.152141437881598E-6	4.152141437881598E-6	0.2308383032390274	4.152141437881598E-6
52-53	0.16200410248182628	1.0587960666598074E-4	4.152141437881598E-6	4.152141437881598E-6	0.23202581569026154	4.152141437881598E-6
54-55	0.16256671764665925	1.0795567738492153E-4	4.152141437881598E-6	4.152141437881598E-6	0.23311160067626757	4.152141437881598E-6
56-57	0.16360890514756754	1.0795567738492153E-4	4.152141437881598E-6	4.152141437881598E-6	0.23420984208658727	4.152141437881598E-6
58-59	0.16377914294652068	1.1003174810386232E-4	4.152141437881598E-6	6.2282121568223966E-6	0.23515030212226745	6.2282121568223966E-6
60-61	0.16409885783723754	1.1210781882280313E-4	8.304282875763195E-6	8.304282875763195E-6	0.23594958934905963	8.304282875763195E-6
62-63	0.16441442058651656	1.1210781882280313E-4	8.304282875763195E-6	8.304282875763195E-6	0.23695648364774594	1.2456424313644791E-5
64-65	0.16443518129370596	1.1418388954174394E-4	8.304282875763195E-6	8.304282875763195E-6	0.23783258549113895	1.2456424313644791E-5
66-67	0.16454313697109088	1.2041210169856633E-4	8.304282875763195E-6	8.304282875763195E-6	0.23854882988917353	1.2456424313644791E-5
68-69	0.1646054190926591	1.2041210169856633E-4	8.304282875763195E-6	8.304282875763195E-6	0.23938133424746877	1.2456424313644791E-5
70-71	0.16475489618442285	1.2248817241750713E-4	8.304282875763195E-6	8.304282875763195E-6	0.2401307957770064	1.868463647046719E-5
72-73	0.16481925437671002	1.2456424313644793E-4	8.304282875763195E-6	8.304282875763195E-6	0.24065604166889842	2.0760707189407987E-5
74-75	0.16485247150821308	1.2664031385538872E-4	8.304282875763195E-6	8.304282875763195E-6	0.24137020999621406	2.0760707189407987E-5
76-77	0.16490437327618657	1.3286852601221113E-4	8.304282875763195E-6	8.304282875763195E-6	0.2421611929401305	2.0760707189407987E-5
78-79	0.16496873146847374	1.370206674500927E-4	8.304282875763195E-6	8.304282875763195E-6	0.24315147867306527	2.0760707189407987E-5
80-81	0.16501855716572833	1.411728088879743E-4	8.304282875763195E-6	8.304282875763195E-6	0.24402965658717723	2.0760707189407987E-5
82-83	0.16504969822651244	1.494770917637375E-4	1.660856575152639E-5	8.304282875763195E-6	0.2447604334802444	2.0760707189407987E-5
84-85	0.16509537178232914	1.494770917637375E-4	2.4912848627289583E-5	8.304282875763195E-6	0.24557632927278814	2.0760707189407987E-5
86-87	0.16512651284311325	1.536292332016191E-4	2.4912848627289583E-5	8.304282875763195E-6	0.24636523614598566	2.0760707189407987E-5
88-89	0.16515142569174052	1.536292332016191E-4	2.4912848627289583E-5	8.304282875763195E-6	0.24706902411970658	2.0760707189407987E-5
90-91	0.165170110328211	1.577813746395007E-4	2.4912848627289583E-5	8.304282875763195E-6	0.24766070427460468	2.0760707189407987E-5
92-93	0.16518256675252463	1.598574453584415E-4	2.6988919346230383E-5	8.304282875763195E-6	0.24822747158087555	2.0760707189407987E-5
94-95	0.16521785995474664	1.619335160773823E-4	2.9064990065171182E-5	8.304282875763195E-6	0.2488876620694987	2.0760707189407987E-5
96-97	0.16521993602546559	1.619335160773823E-4	2.9064990065171182E-5	8.304282875763195E-6	0.24951048328518094	2.0760707189407987E-5
98-99	0.16525522922768757	1.619335160773823E-4	3.321713150305278E-5	8.304282875763195E-6	0.250104239510798	2.4912848627289583E-5
100-101	0.16530713099566108	1.619335160773823E-4	3.321713150305278E-5	1.2456424313644791E-5	0.25065024610987946	2.4912848627289583E-5
102-103	0.16534034812716414	1.702377989531455E-4	3.321713150305278E-5	1.2456424313644791E-5	0.2512024809211177	2.4912848627289583E-5
104-105	0.16536110883435357	1.743899403910271E-4	3.321713150305278E-5	1.2456424313644791E-5	0.2517983132174537	2.4912848627289583E-5
106-107	0.16538186954154296	1.8061815254784947E-4	3.321713150305278E-5	1.2456424313644791E-5	0.25240037372594654	2.4912848627289583E-5
108-109	0.16541508667304602	1.8684636470467188E-4	3.321713150305278E-5	1.2456424313644791E-5	0.25296506496149845	2.4912848627289583E-5
110-111	0.16546076022886272	1.909985061425535E-4	3.321713150305278E-5	1.2456424313644791E-5	0.2535629733285534	2.6988919346230383E-5
112-113	0.16547736879461422	1.909985061425535E-4	3.321713150305278E-5	1.2456424313644791E-5	0.2541816424027977	2.9064990065171182E-5
114-115	0.1655002055725226	1.909985061425535E-4	3.321713150305278E-5	1.2456424313644791E-5	0.25475463792122544	2.9064990065171182E-5
116-117	0.16551889020899307	1.909985061425535E-4	3.321713150305278E-5	1.2456424313644791E-5	0.2553089488031826	2.9064990065171182E-5
118-119	0.1655375748454635	1.9930278901831666E-4	3.321713150305278E-5	1.2456424313644791E-5	0.2558736400387345	2.9064990065171182E-5
120-121	0.16554380305762034	2.0553100117513907E-4	3.321713150305278E-5	1.2456424313644791E-5	0.25650268946657356	2.9064990065171182E-5
122-123	0.1655521073404961	2.1175921333196147E-4	3.321713150305278E-5	1.660856575152639E-5	0.2569864139440868	2.9064990065171182E-5
124-125	0.16556248769409082	2.1175921333196147E-4	3.321713150305278E-5	1.660856575152639E-5	0.2575137359066977	3.321713150305278E-5
126-127	0.16557494411840445	2.1175921333196147E-4	3.321713150305278E-5	1.868463647046719E-5	0.25813448105166104	3.736927294093438E-5
128-129	0.1655977808963128	2.1175921333196147E-4	3.321713150305278E-5	2.0760707189407987E-5	0.2588465733082577	4.1521414378815974E-5
130-131	0.16560400910846962	2.1591135476984306E-4	3.321713150305278E-5	2.0760707189407987E-5	0.2595316766455082	4.1521414378815974E-5
132-133	0.1656226937449401	2.1591135476984306E-4	3.321713150305278E-5	2.0760707189407987E-5	0.2601565739319094	4.1521414378815974E-5
134-135	0.16563515016925373	2.1591135476984306E-4	3.7369272940934374E-5	2.0760707189407987E-5	0.26073579766249383	4.567355581669757E-5
136-137	0.16564137838141058	2.2213956692666547E-4	3.7369272940934374E-5	2.0760707189407987E-5	0.2612880324737321	4.567355581669757E-5
138-139	0.16566629123003784	2.2836777908348787E-4	3.7369272940934374E-5	2.0760707189407987E-5	0.2617696808805264	5.1901767973519965E-5
>>END_MODULE
