##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.4d.R.1.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23055046
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.82475576062611	37.0	35.0	38.0	33.0	39.0
2	35.34516877563376	35.0	34.0	37.0	33.0	39.0
3	35.48216186599671	35.0	34.0	37.0	33.0	39.0
4	35.56975197533763	36.0	35.0	38.0	33.0	39.0
5	35.49805986073504	36.0	35.0	37.0	33.0	39.0
6	35.438631742482755	36.0	35.0	37.0	33.0	39.0
7	35.40592493287586	35.0	34.0	37.0	33.0	39.0
8	35.55656484051257	36.0	34.0	38.0	33.0	39.0
9	35.52775791468818	36.0	34.0	38.0	33.0	39.0
10-14	35.45157792354871	36.0	34.4	37.6	33.0	39.0
15-19	35.455950892485745	36.0	34.0	38.0	33.0	39.0
20-24	35.43408229157296	36.0	34.0	38.0	33.0	39.0
25-29	35.41399101090495	36.0	34.0	38.0	33.0	39.0
30-34	35.38571430306407	36.0	34.0	38.0	33.0	39.0
35-39	35.358903460873606	36.0	34.0	37.8	32.4	39.0
40-44	35.33757555721207	36.0	34.0	37.6	32.0	39.0
45-49	35.30119853154923	36.0	34.0	37.4	32.0	39.0
50-54	35.27614087605811	36.0	34.0	37.2	32.0	39.0
55-59	35.241245296149046	36.0	34.0	37.0	32.0	39.0
60-64	35.19722321091878	36.0	34.0	37.0	32.0	39.0
65-69	35.15563168253926	35.8	34.0	37.0	32.0	39.0
70-74	35.12014568958136	35.8	34.0	37.0	32.0	39.0
75-79	35.06971672058255	35.0	34.0	37.0	32.0	39.0
80-84	35.02436759397487	35.0	34.0	37.0	32.0	39.0
85-89	34.97527527813217	35.0	34.0	37.0	31.6	39.0
90-94	34.910820338419626	35.0	34.0	37.0	31.0	39.0
95-99	34.8415565859205	35.0	34.0	37.0	31.0	39.0
100-104	34.781014238705055	35.0	34.0	37.0	31.0	39.0
105-109	34.708902979417175	35.0	34.0	37.0	31.0	39.0
110-114	34.63735890182132	35.0	34.0	37.0	30.2	39.0
115-119	34.55870301885322	35.0	34.0	37.0	30.0	39.0
120-124	34.47375383245819	35.0	34.0	37.0	30.0	39.0
125-129	34.40333125338375	35.0	34.0	37.0	30.0	39.0
130-134	34.30131481845666	35.0	34.0	37.0	29.8	39.0
135-139	34.19779861640701	35.0	34.0	37.0	29.0	39.0
140-144	34.08889222775786	35.0	34.0	37.0	29.0	39.0
145-149	33.98834474674221	35.0	33.8	37.0	28.4	39.0
150	33.91701361168397	35.0	33.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	26.0
21	273.0
22	2524.0
23	12451.0
24	39800.0
25	91536.0
26	166359.0
27	263490.0
28	367913.0
29	477102.0
30	610552.0
31	796602.0
32	1074292.0
33	1522201.0
34	2344190.0
35	4108676.0
36	9806257.0
37	1370797.0
38	5.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.78738910071771	19.002204333533825	16.636869134173303	31.57353743157516
2	24.196572845701546	25.49831390490394	33.262241159701006	17.04287208969351
3	19.74120546105178	29.55495295910492	30.624263339140594	20.07957824070271
4	23.30480045928344	34.076629825560076	22.45716815808289	20.161401557073585
5	25.187710542312974	35.30302803572927	22.44758142957431	17.061679992383446
6	18.730402880133052	39.36180825663935	22.62958399649257	19.27820486673503
7	20.192646763749682	20.714844810979773	38.317485898748586	20.775022526521962
8	21.332379905032504	23.349812444529498	28.468127975107922	26.84967967533008
9	23.437577328519637	23.121767925415025	28.775406857804875	24.665247888260467
10-14	22.95185150970063	29.369785349032686	25.680818878810374	21.997544262456305
15-19	22.840616050142327	28.42548989298455	27.10276337169997	21.631130685173147
20-24	22.93921619535451	28.51478525364716	26.988739271990514	21.55725927900781
25-29	23.071356266216082	28.432986861097564	26.90439914975663	21.591257722929722
30-34	23.061279713441298	28.223313267067983	27.079633908165995	21.635773111324724
35-39	23.164993034496657	28.171830149460558	27.039787298624347	21.623389517418442
40-44	23.31706213034665	27.943494886108667	27.155854731324325	21.58358825222036
45-49	23.329700578346277	27.766562686537256	27.238636609096332	21.665100126020135
50-54	23.404901469292234	27.725544334199114	27.188068069783945	21.681486126724707
55-59	23.561635921264266	27.607274346795922	27.09948789518789	21.73160183675192
60-64	23.476298420744858	27.583696861849678	27.167223175351722	21.772781542053742
65-69	23.57899437719621	27.503477546737493	27.118776514260695	21.798751561805602
70-74	23.66262205679399	27.441170145572467	27.03968146495999	21.85652633267355
75-79	23.578753215239736	27.505631522053786	27.10930440130113	21.80631086140535
80-84	23.712380361331746	27.54966786880408	26.95273563973804	21.785216130126134
85-89	23.731707355792807	27.482028250839562	27.018004882905068	21.768259510462563
90-94	23.684043831445837	27.41536061129525	27.05730710751998	21.843288449738942
95-99	23.746747588133033	27.536603659717958	27.0654989499825	21.651149802166504
100-104	23.923226177904827	27.488618326764563	26.882469026520265	21.70568646881034
105-109	23.688259828238902	27.629157625623474	26.99260543657124	21.68997710956638
110-114	23.72876136294641	27.61775298663688	26.909783020253208	21.743702630163504
115-119	23.79266040067758	27.6237436264495	26.924932615619156	21.658663357253765
120-124	23.759561355895798	27.54542931729566	27.08969567876811	21.605313648040433
125-129	23.753564610138405	27.635524367511778	27.04184781970808	21.56906320264174
130-134	23.795519851016735	27.530785473434193	27.126582416071365	21.547112259477704
135-139	23.698897846484453	27.529639281569857	27.228559856267477	21.542903015678217
140-144	23.74467784622941	27.657210486589356	27.18421815337085	21.413893513810383
145-149	23.83537035666726	28.18727058709837	26.69718203988836	21.28017701634601
150	23.462963379036417	28.16510971177416	26.82496924968183	21.546957659507598
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	10.0
3	25.0
4	35.5
5	45.0
6	48.0
7	39.0
8	28.0
9	19.5
10	12.5
11	14.0
12	18.5
13	15.5
14	23.0
15	45.5
16	78.0
17	134.0
18	207.5
19	382.0
20	650.5
21	985.5
22	1460.5
23	2510.0
24	4336.0
25	7034.0
26	10853.0
27	17424.0
28	28960.5
29	43573.0
30	58185.5
31	76345.5
32	102539.5
33	136046.0
34	180951.5
35	246533.5
36	343613.0
37	461962.0
38	599224.0
39	777275.0
40	985928.5
41	1177792.5
42	1348965.0
43	1504282.0
44	1613115.5
45	1662315.0
46	1658598.5
47	1602981.5
48	1503135.5
49	1364255.5
50	1185743.5
51	994359.0
52	811660.5
53	646950.5
54	504921.5
55	381948.0
56	282191.0
57	209729.0
58	153895.5
59	110013.5
60	76331.5
61	51030.0
62	34724.0
63	24625.5
64	17329.0
65	12386.5
66	9154.5
67	6764.5
68	5607.0
69	4178.0
70	2511.5
71	1807.0
72	1362.5
73	1072.0
74	907.5
75	552.5
76	181.5
77	68.0
78	22.5
79	2.5
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.1687226301782263E-5
2	0.0
3	0.0
4	8.674890520712906E-6
5	4.337445260356453E-6
6	0.0
7	0.0
8	0.0
9	4.337445260356453E-6
10-14	0.0959815911883238
15-19	2.6024671562138715E-6
20-24	8.674890520712907E-7
25-29	0.0
30-34	8.674890520712907E-7
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	8.674890520712907E-7
90-94	0.0
95-99	6.9399124165703255E-6
100-104	0.0
105-109	0.0
110-114	8.674890520712907E-7
115-119	0.0
120-124	0.0
125-129	8.674890520712907E-7
130-134	8.674890520712907E-7
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.3055046E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.87240444405474
#Duplication Level	Percentage of total
1	24.83513780261682
2	11.177411723513147
3	7.487732017918
4	5.358017688931184
5	4.076531611413866
6	3.2732692226806135
7	2.7079639759478433
8	2.334607855956618
9	1.9824298561565832
>10	22.450819058849465
>50	5.7847168327659375
>100	6.52522305108405
>500	0.9950361879990735
>1k	0.9096428118611513
>5k	0.05516914991155754
>10k+	0.04629115239413427
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.337445260356453E-6	4.337445260356453E-6	4.337445260356453E-6	0.0	0.0014270194906572731	0.0
2	1.3012335781069358E-5	3.4699562082851625E-5	4.337445260356453E-6	0.0	0.005937962561427984	0.0
3	4.771189786392098E-5	3.903700734320808E-5	4.337445260356453E-6	0.0	0.007759689570777694	0.0
4	9.542379572784196E-5	3.903700734320808E-5	8.674890520712906E-6	8.674890520712906E-6	0.009178034170914255	0.0
5	9.976124098819841E-5	3.903700734320808E-5	8.674890520712906E-6	8.674890520712906E-6	0.010739514464642578	0.0
6	1.0409868624855487E-4	3.903700734320808E-5	8.674890520712906E-6	8.674890520712906E-6	0.012465817678264445	0.0
7	1.0843613150891132E-4	5.638678838463389E-5	8.674890520712906E-6	8.674890520712906E-6	0.014621527972661603	0.0
8	1.5614802937283231E-4	5.638678838463389E-5	8.674890520712906E-6	8.674890520712906E-6	0.0202992438184682	0.0
9	1.6482291989354522E-4	5.638678838463389E-5	8.674890520712906E-6	8.674890520712906E-6	0.027043971198322483	0.0
10-11	1.9084759145568392E-4	5.638678838463389E-5	8.674890520712906E-6	8.674890520712906E-6	0.034209430768431345	0.0
12-13	2.212097082781791E-4	5.638678838463389E-5	8.674890520712906E-6	8.674890520712906E-6	0.04066354931584175	0.0
14-15	2.4940310247049605E-4	6.50616789053468E-5	8.674890520712906E-6	8.674890520712906E-6	0.049967369399306336	0.0
16-17	2.689216061421001E-4	6.939912416570325E-5	8.674890520712906E-6	8.674890520712906E-6	0.056067986157997685	0.0
18-19	3.1446478137584286E-4	6.939912416570325E-5	8.674890520712906E-6	1.3012335781069358E-5	0.0647819136860538	0.0
20-21	3.621766792397638E-4	7.37365694260597E-5	8.674890520712906E-6	1.3012335781069358E-5	0.0708478308826623	0.0
22-23	3.92538796062259E-4	9.542379572784196E-5	8.674890520712906E-6	1.5181058411247585E-5	0.07722604413801647	0.0
24-25	4.445881391865364E-4	9.759251835802019E-5	8.674890520712906E-6	2.1687226301782266E-5	0.0830295458963734	0.0
26-27	5.009749275711703E-4	1.0409868624855487E-4	8.674890520712906E-6	2.1687226301782266E-5	0.08748627090138966	0.0
28-29	5.855551101481212E-4	1.1277357676926778E-4	1.0843613150891131E-5	2.1687226301782266E-5	0.09198853908163966	0.0
30-31	7.330282490002405E-4	1.14942299399446E-4	1.3012335781069358E-5	2.1687226301782266E-5	0.0974016707665645	0.0
32-33	8.52307993660043E-4	1.2795463518051536E-4	1.3012335781069358E-5	2.1687226301782266E-5	0.10303601215976754	0.0
34-35	9.824313514707366E-4	1.301233578106936E-4	1.3012335781069358E-5	2.1687226301782266E-5	0.1062674088787331	0.0
36-37	0.0010366494172251923	1.4313569359176295E-4	1.3012335781069358E-5	2.1687226301782266E-5	0.10999978052526982	0.0
38-39	0.0010886987603494697	1.4964186148229763E-4	1.3012335781069358E-5	2.1687226301782266E-5	0.11362154731766747	0.0
40-41	0.001119060877171965	1.62654197263367E-4	1.3012335781069358E-5	2.1687226301782266E-5	0.1164864299121329	0.0
42-43	0.0011364106582133907	1.8434142356514924E-4	1.3012335781069358E-5	2.1687226301782266E-5	0.1194055305723528	0.0
44-45	0.0011602666071453512	2.1036609512728796E-4	1.3012335781069358E-5	2.1687226301782266E-5	0.12197763561174417	0.0
46-47	0.0012101472276394504	2.4072821194978314E-4	1.3012335781069358E-5	2.1687226301782266E-5	0.1245931151037391	2.1687226301782265E-6
48-49	0.0012405093444619456	2.450656572101396E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.12721943820888493	6.506167890534679E-6
50-51	0.0013077397459974706	2.5590927036103073E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.12961153926997154	8.674890520712906E-6
52-53	0.0013619578117519263	2.5590927036103073E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.13203617117051078	8.674890520712906E-6
54-55	0.0013901512059442433	2.602467156213872E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.134107301282331	8.674890520712906E-6
56-57	0.0014400318264383425	2.6675288351192186E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.1364777151171158	8.674890520712906E-6
58-59	0.0014834062790419068	2.7325905140245654E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.13862041307573186	8.674890520712906E-6
60-61	0.0015549741258377883	2.949462777042388E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.1401406876394868	8.674890520712906E-6
62-63	0.0015961798558111746	3.1229605874566463E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.14212073140083953	8.674890520712906E-6
64-65	0.0016352168631543827	3.2964583978709045E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.14396414563649101	1.0843613150891131E-5
66-67	0.001704615987320086	3.600079566095856E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.1454909263681365	1.3012335781069358E-5
68-69	0.0017349781041425811	3.643454018699421E-4	1.3012335781069358E-5	2.6024671562138717E-5	0.1470155383771518	1.5181058411247585E-5
70-71	0.0017675089435952545	3.795264602811896E-4	1.7349781041425812E-5	2.6024671562138717E-5	0.14848159487515228	2.1687226301782266E-5
72-73	0.0018217270093497102	3.947075186924372E-4	1.7349781041425812E-5	2.6024671562138717E-5	0.14949005089818515	2.6024671562138717E-5
74-75	0.0018455829582816707	3.9904496395279365E-4	1.7349781041425812E-5	2.6024671562138717E-5	0.15057441221327428	2.8193394192316944E-5
76-77	0.00185859529406274	4.337445260356453E-4	2.6024671562138717E-5	2.6024671562138717E-5	0.1519363700250262	3.036211682249517E-5
78-79	0.0018889574108852353	4.662753654883187E-4	3.036211682249517E-5	2.6024671562138717E-5	0.15355857455239952	3.036211682249517E-5
80-81	0.0018998010240361264	4.923000370504574E-4	3.036211682249517E-5	2.6024671562138717E-5	0.15487282046628753	3.036211682249517E-5
82-83	0.0019019697466663046	5.053123728315268E-4	3.036211682249517E-5	2.6024671562138717E-5	0.1565752677309774	3.036211682249517E-5
84-85	0.0019193195277077304	5.096498180918832E-4	3.4699562082851625E-5	2.6024671562138717E-5	0.15824735287884484	3.036211682249517E-5
86-87	0.0019366693087491563	5.118185407220615E-4	3.4699562082851625E-5	2.8193394192316944E-5	0.16006907988819452	3.036211682249517E-5
88-89	0.0019453441992698692	5.139872633522397E-4	3.4699562082851625E-5	3.036211682249517E-5	0.1619775558027514	3.036211682249517E-5
90-91	0.0019475129219000474	5.291683217634873E-4	3.4699562082851625E-5	3.036211682249517E-5	0.16311830390622511	3.036211682249517E-5
92-93	0.0019475129219000474	5.291683217634873E-4	3.4699562082851625E-5	3.036211682249517E-5	0.1641441097102994	3.4699562082851625E-5
94-95	0.0019561878124207603	5.291683217634873E-4	3.4699562082851625E-5	3.036211682249517E-5	0.16525449569695067	3.686828471302985E-5
96-97	0.001973537593462186	5.400119349143784E-4	3.4699562082851625E-5	3.036211682249517E-5	0.16630415744995694	3.903700734320808E-5
98-99	0.0019843812066130772	5.465181028049131E-4	3.4699562082851625E-5	3.036211682249517E-5	0.16728008263353716	3.903700734320808E-5
100-101	0.001990887374503612	5.508555480652695E-4	3.4699562082851625E-5	3.036211682249517E-5	0.16813239062719718	3.903700734320808E-5
102-103	0.001990887374503612	5.530242706954478E-4	3.686828471302985E-5	3.036211682249517E-5	0.1689998796792685	4.771189786392098E-5
104-105	0.0019952248197639684	5.595304385859825E-4	3.903700734320808E-5	3.036211682249517E-5	0.16986953745396993	5.2049343124277434E-5
106-107	0.002021249491326107	5.725427743670518E-4	3.903700734320808E-5	3.036211682249517E-5	0.17068714588554715	5.2049343124277434E-5
108-109	0.0020320931044769982	5.83386387517943E-4	3.903700734320808E-5	3.036211682249517E-5	0.17150258559449416	5.638678838463389E-5
110-111	0.0020429367176278894	6.072423364499034E-4	4.337445260356453E-5	3.036211682249517E-5	0.17235923103341455	6.072423364499034E-5
112-113	0.002047274162888246	6.332670080120422E-4	4.337445260356453E-5	3.036211682249517E-5	0.17307707822400353	7.156784679588148E-5
114-115	0.002047274162888246	6.419418985327551E-4	4.337445260356453E-5	3.036211682249517E-5	0.17394239855344468	7.590529205623793E-5
116-117	0.0020537803307787805	6.50616789053468E-4	4.337445260356453E-5	3.036211682249517E-5	0.17488796162020237	8.024273731659438E-5
118-119	0.0020559490534089587	6.527855116836462E-4	4.337445260356453E-5	3.036211682249517E-5	0.17569472643862866	8.891762783730729E-5
120-121	0.0020559490534089587	6.549542343138244E-4	4.337445260356453E-5	3.4699562082851625E-5	0.1765795652717414	9.108635046748552E-5
122-123	0.002060286498669315	6.549542343138244E-4	4.554317523374275E-5	3.4699562082851625E-5	0.17731042479811143	9.325507309766373E-5
124-125	0.00206679266655985	6.592916795741809E-4	5.2049343124277434E-5	3.4699562082851625E-5	0.17816923895966202	9.759251835802019E-5
126-127	0.0020841424476012757	6.636291248345373E-4	5.2049343124277434E-5	3.4699562082851625E-5	0.17916685136954402	1.0192996361837664E-4
128-129	0.0020993235060125233	6.679665700948938E-4	5.2049343124277434E-5	3.4699562082851625E-5	0.18017530739257687	1.0409868624855487E-4
130-131	0.0021036609512728797	6.939912416570325E-4	5.421806575445566E-5	3.4699562082851625E-5	0.18107315856147066	1.0409868624855487E-4
132-133	0.0021123358417935926	7.004974095475672E-4	5.638678838463389E-5	3.4699562082851625E-5	0.18209245819765443	1.0409868624855487E-4
134-135	0.0021210107323143055	7.070035774381019E-4	5.638678838463389E-5	3.4699562082851625E-5	0.182914404074492	1.14942299399446E-4
136-137	0.0021231794549444838	7.070035774381019E-4	5.8555511014812115E-5	3.4699562082851625E-5	0.1838100865207556	1.2144846728998068E-4
138-139	0.0021383605133557314	7.135097453286366E-4	6.072423364499034E-5	3.4699562082851625E-5	0.18454094604712565	1.2144846728998068E-4
>>END_MODULE
