##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.4d.R.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22208574
Total Bases	3.3 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.05806658275313	37.0	35.0	38.0	33.0	39.0
2	35.53527943757217	35.0	34.0	38.0	33.0	39.0
3	35.64588118084484	36.0	35.0	38.0	33.0	39.0
4	35.763041337098	36.0	35.0	38.0	33.0	39.0
5	35.69356852898345	36.0	35.0	38.0	33.0	39.0
6	35.61464027361685	36.0	35.0	37.0	33.0	39.0
7	35.56928697898388	35.0	34.0	38.0	33.0	39.0
8	35.74822368153849	36.0	35.0	38.0	33.0	39.0
9	35.72761434390159	36.0	35.0	38.0	33.0	39.0
10-14	35.65025731953794	36.0	35.0	37.8	33.0	39.0
15-19	35.659660381616575	36.0	35.0	38.0	33.0	39.0
20-24	35.640620203710505	36.0	35.0	38.0	33.0	39.0
25-29	35.62747757690341	36.0	35.0	38.0	33.0	39.0
30-34	35.60176822699197	36.0	35.0	38.0	33.0	39.0
35-39	35.581270999209586	36.0	35.0	38.0	33.0	39.0
40-44	35.5629953008239	36.0	35.0	38.0	33.0	39.0
45-49	35.53382666532304	36.0	35.0	38.0	33.0	39.0
50-54	35.51266994449981	36.0	35.0	38.0	33.0	39.0
55-59	35.48532129077715	36.0	34.8	38.0	33.0	39.0
60-64	35.44779654920662	36.0	34.0	38.0	33.0	39.0
65-69	35.413679023245706	36.0	34.0	38.0	33.0	39.0
70-74	35.38402579112013	36.0	34.0	38.0	32.2	39.0
75-79	35.341515983871815	36.0	34.0	38.0	32.0	39.0
80-84	35.304501810877184	36.0	34.0	38.0	32.0	39.0
85-89	35.26270659250792	36.0	34.0	38.0	32.0	39.0
90-94	35.207633556301275	36.0	34.0	38.0	32.0	39.0
95-99	35.14815949011404	36.0	34.0	38.0	32.0	39.0
100-104	35.098735623457856	36.0	34.0	38.0	32.0	39.0
105-109	35.03582592921094	36.0	34.0	38.0	32.0	39.0
110-114	34.97444354599264	35.8	34.0	37.6	31.2	39.0
115-119	34.90638246291725	35.0	34.0	37.0	31.0	39.0
120-124	34.831197500568926	35.0	34.0	37.0	31.0	39.0
125-129	34.77086808905425	35.0	34.0	37.0	31.0	39.0
130-134	34.68257702633226	35.0	34.0	37.0	30.2	39.0
135-139	34.591211961650494	35.0	34.0	37.0	30.0	39.0
140-144	34.49287296879125	35.0	34.0	37.0	30.0	39.0
145-149	34.40526485851815	35.0	34.0	37.0	30.0	39.0
150	34.341238298325685	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	14.0
21	207.0
22	1824.0
23	8772.0
24	28295.0
25	66750.0
26	128423.0
27	211375.0
28	301321.0
29	389164.0
30	494582.0
31	642477.0
32	866721.0
33	1238672.0
34	1942192.0
35	3528024.0
36	1.0509584E7
37	1850168.0
38	9.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.349555297837256	18.78674144379927	16.84348277773105	31.02022048063243
2	24.81428568984213	24.89167922262816	33.09660043909168	17.19743464843803
3	20.178621103723273	29.079165551106524	30.770489811727668	19.971723533442535
4	23.89870686879761	33.547885604901964	22.51636237427941	20.037045152021015
5	25.730121168518068	35.104824830266004	22.29445258394348	16.870601417272447
6	19.03465751560636	39.39080465049219	22.50203907734013	19.072498756561316
7	20.46376865366361	20.879644090595104	38.1799677088663	20.476619546874982
8	21.49357367535501	23.415984448888274	28.515641234580897	26.574800641175823
9	23.696298555683946	23.10310873629257	28.793523618400712	24.407069089622773
10-14	23.13007912966429	29.47656008548165	25.653387073518523	21.739973711335537
15-19	23.077749161202334	28.384145690758896	27.096733901060016	21.441371246978758
20-24	23.162708449112568	28.43605472765658	27.00354761186871	21.397689211362145
25-29	23.287449252707535	28.364305605573776	26.9022684662239	21.445976675494787
30-34	23.223844088323727	28.173108277911048	27.122761686545026	21.480285947220203
35-39	23.334741107958944	28.105649899949903	27.08045664778348	21.479152344307675
40-44	23.454619823857218	27.896167489186833	27.200486622869168	21.448726064086777
45-49	23.47818009386825	27.70766371582435	27.274548109212233	21.539608081095167
50-54	23.521595281562792	27.683787850292198	27.245314845434287	21.549302022710727
55-59	23.690136070870647	27.562725098873976	27.158506439900194	21.588632390355183
60-64	23.57979400208226	27.542827378290923	27.226339701054197	21.65103891857262
65-69	23.691703933805027	27.453785191250912	27.204484178047633	21.650026696896433
70-74	23.776264068102705	27.386299543590688	27.116716273633777	21.72072011467283
75-79	23.693419487446604	27.446376340957322	27.199304196658463	21.660899974937607
80-84	23.818219035585088	27.491742603554826	27.02800008681332	21.662038274046772
85-89	23.829110324688113	27.459855819648755	27.08463587081278	21.626397984850357
90-94	23.78439786363591	27.376525840875694	27.138633934803742	21.70044236068466
95-99	23.83809733149534	27.4922682109496	27.14633062778352	21.523303829771535
100-104	24.02660835453205	27.438343001011106	26.956295833425344	21.578752811031503
105-109	23.799165347574007	27.579575596160073	27.07242911744292	21.548829938822998
110-114	23.842620422184694	27.57353443764557	26.98185844800301	21.601986692166726
115-119	23.906406597740133	27.57721679924159	27.005986967015534	21.510389636002746
120-124	23.88148829366532	27.504104495858222	27.15315625397651	21.461250956499953
125-129	23.856098099769934	27.60047538396657	27.110486247338528	21.432940268924966
130-134	23.921849282173632	27.506723304251775	27.1593070315996	21.412120381974997
135-139	23.810848222950725	27.514152214492942	27.255471938499714	21.419527624056617
140-144	23.850529979997816	27.63598239130527	27.22195670915206	21.291530919544858
145-149	23.96232914369018	28.180031730087666	26.711228735352393	21.14641039086976
150	23.563228327942173	28.151474290965282	26.85642040772181	21.428876973370738
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	2.0
2	10.0
3	23.5
4	31.0
5	37.0
6	38.0
7	37.0
8	31.5
9	24.5
10	20.0
11	17.0
12	16.5
13	16.0
14	25.5
15	43.0
16	62.0
17	117.0
18	200.5
19	343.5
20	602.5
21	994.5
22	1476.0
23	2402.5
24	3953.0
25	6390.5
26	10079.0
27	16039.5
28	26219.0
29	39341.0
30	53537.0
31	70981.5
32	95053.0
33	127548.5
34	170876.5
35	235202.5
36	329462.5
37	444084.0
38	578541.0
39	750566.0
40	951018.0
41	1135196.5
42	1298718.5
43	1453728.0
44	1565515.0
45	1612716.5
46	1604748.0
47	1548415.5
48	1449458.0
49	1310669.5
50	1137465.0
51	953916.5
52	778644.5
53	619495.5
54	483206.5
55	365929.5
56	270886.0
57	201712.5
58	147980.0
59	106384.5
60	74529.5
61	50101.5
62	34470.0
63	24552.5
64	17030.5
65	12147.0
66	9105.5
67	6837.0
68	5876.0
69	4494.5
70	2722.0
71	2001.5
72	1502.0
73	1124.0
74	971.0
75	581.0
76	182.5
77	66.5
78	20.0
79	3.5
80	2.5
81	2.0
82	1.5
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.005530926929392E-6
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	4.502765463464696E-6
8	4.502765463464696E-6
9	0.0
10-14	0.09827285624011699
15-19	0.0
20-24	9.005530926929391E-7
25-29	0.0
30-34	0.0
35-39	9.005530926929391E-7
40-44	0.0
45-49	0.0
50-54	1.8011061853858782E-6
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	5.403318556157635E-6
100-104	1.8011061853858782E-6
105-109	9.005530926929391E-7
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	9.005530926929391E-7
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.2208574E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.06258675721007
#Duplication Level	Percentage of total
1	25.80463979023868
2	11.549923547415926
3	7.603095953058118
4	5.4027439581086565
5	4.099041753180876
6	3.3549653863641393
7	2.7086647287816144
8	2.2836344596832348
9	1.9593964724505097
>10	22.089987126861892
>50	5.416507498257379
>100	6.094485739992192
>500	0.8115263327106197
>1k	0.7306464804144556
>5k	0.09074077248149794
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.502765463464696E-6	4.502765463464696E-6	0.0	0.0	0.0014048628246009851	0.0
2	1.3508296390394089E-5	3.602212370771757E-5	0.0	9.005530926929392E-6	0.005592434705623152	0.0
3	4.502765463464696E-5	3.602212370771757E-5	0.0	9.005530926929392E-6	0.007168402617835796	0.0
4	1.0356360565968801E-4	3.602212370771757E-5	0.0	9.005530926929392E-6	0.00851472949141174	0.0
5	1.0806637112315271E-4	3.602212370771757E-5	4.502765463464696E-6	9.005530926929392E-6	0.01023028313299179	0.0
6	1.1256913658661741E-4	3.602212370771757E-5	4.502765463464696E-6	9.005530926929392E-6	0.011797245514277504	0.0
7	1.350829639039409E-4	4.9530420098111654E-5	9.005530926929392E-6	9.005530926929392E-6	0.013796473380055829	0.0
8	1.8011061853858784E-4	5.853595102504105E-5	9.005530926929392E-6	9.005530926929392E-6	0.019046697910455664	0.0
9	1.8461338400205254E-4	7.204424741543513E-5	9.005530926929392E-6	9.005530926929392E-6	0.0246031104923711	0.0
10-11	2.3864656956362886E-4	8.330116107409688E-5	9.005530926929392E-6	9.005530926929392E-6	0.031006042981417896	0.0
12-13	2.814228414665435E-4	8.555254380582922E-5	9.005530926929392E-6	9.005530926929392E-6	0.036960950306849955	0.0
14-15	3.196963479059934E-4	9.906084019622332E-5	9.005530926929392E-6	9.005530926929392E-6	0.04489707443620648	0.0
16-17	3.5571847161371096E-4	1.0581498839142036E-4	9.005530926929392E-6	9.005530926929392E-6	0.05060658104387972	0.0
18-19	4.0975165717528736E-4	1.2382605024527913E-4	9.005530926929392E-6	9.005530926929392E-6	0.05855396208689491	0.0
20-21	4.5928207727339904E-4	1.305801984404762E-4	9.005530926929392E-6	9.005530926929392E-6	0.06429723943554413	0.0
22-23	4.975555837128489E-4	1.8011061853858784E-4	9.005530926929392E-6	9.005530926929392E-6	0.07004276816692509	0.0
24-25	5.358290901522989E-4	1.913675321972496E-4	9.005530926929392E-6	9.005530926929392E-6	0.07520969153625082	0.0
26-27	5.718512138600165E-4	1.9812168039244662E-4	9.005530926929392E-6	9.005530926929392E-6	0.07894698687092652	0.0
28-29	6.844203504466338E-4	1.9812168039244662E-4	9.005530926929392E-6	9.005530926929392E-6	0.08285538729321387	0.0
30-31	8.73536499912151E-4	1.9812168039244662E-4	9.005530926929392E-6	9.005530926929392E-6	0.08763057006721818	0.0
32-33	0.001015373612011289	2.2513827317323482E-4	9.005530926929392E-6	9.005530926929392E-6	0.09288980012854495	4.502765463464696E-6
34-35	0.0011346968967931035	2.3639518683189655E-4	1.125691365866174E-5	9.005530926929392E-6	0.09587738501355378	4.502765463464696E-6
36-37	0.0011752217859642856	2.431493350270936E-4	1.3508296390394089E-5	9.005530926929392E-6	0.09915539827095607	4.502765463464696E-6
38-39	0.0012292549715258619	2.431493350270936E-4	1.3508296390394089E-5	9.005530926929392E-6	0.10236361866367466	4.502765463464696E-6
40-41	0.0012652770952335797	2.5665763141748766E-4	1.5759679122126437E-5	9.005530926929392E-6	0.10520036090565743	4.502765463464696E-6
42-43	0.001299047836209565	2.724173105396141E-4	1.8011061853858784E-5	1.3508296390394089E-5	0.10783222731905254	4.502765463464696E-6
44-45	0.0013328185771855502	2.8367422419827587E-4	1.8011061853858784E-5	1.3508296390394089E-5	0.1100003088897108	4.502765463464696E-6
46-47	0.0013755948490884646	3.219477306377258E-4	1.8011061853858784E-5	1.3508296390394089E-5	0.1122764568314922	4.502765463464696E-6
48-49	0.0014228738864548438	3.3545602702811985E-4	1.8011061853858784E-5	1.3508296390394089E-5	0.11470795018176314	4.502765463464696E-6
50-51	0.0014836612202116174	3.422101752233169E-4	1.8011061853858784E-5	1.3508296390394089E-5	0.11677021676402996	4.502765463464696E-6
52-53	0.0015196833439193348	3.4446155795504925E-4	1.8011061853858784E-5	1.3508296390394089E-5	0.11869514899966112	4.502765463464696E-6
54-55	0.0015962303567982348	3.5121570615024634E-4	1.8011061853858784E-5	1.3508296390394089E-5	0.12043771923402197	4.502765463464696E-6
56-57	0.0016367552459694169	3.692267680041051E-4	1.8011061853858784E-5	1.3508296390394089E-5	0.12237615976604352	4.502765463464696E-6
58-59	0.0016750287524088668	3.7372953346756976E-4	1.8011061853858784E-5	1.3508296390394089E-5	0.12423580190245442	4.502765463464696E-6
60-61	0.001726810555238711	3.894892125896962E-4	1.8011061853858784E-5	1.3508296390394089E-5	0.12555060941778612	9.005530926929392E-6
62-63	0.0017448216170925697	3.9624336078489323E-4	1.8011061853858784E-5	1.5759679122126437E-5	0.12735621836863548	9.005530926929392E-6
64-65	0.0017583299134829638	4.07500274443555E-4	1.8011061853858784E-5	1.8011061853858784E-5	0.1290920344548011	1.125691365866174E-5
66-67	0.0018191172472397372	4.367682499560755E-4	2.026244458559113E-5	1.8011061853858784E-5	0.13041134473559626	1.3508296390394089E-5
68-69	0.0018416310745570607	4.5027654634646964E-4	2.251382731732348E-5	1.8011061853858784E-5	0.1317576716091722	1.5759679122126437E-5
70-71	0.001861893519142652	4.5252792907820194E-4	2.251382731732348E-5	1.8011061853858784E-5	0.13304095976625963	1.8011061853858784E-5
72-73	0.0018844073464599753	4.7504175639552547E-4	2.251382731732348E-5	1.8011061853858784E-5	0.13389423382158622	1.8011061853858784E-5
74-75	0.0019114239392407635	4.885500527859195E-4	3.1519358244252874E-5	1.8011061853858784E-5	0.13491185881632922	2.251382731732348E-5
76-77	0.001940691914753284	5.065611146397783E-4	3.602212370771757E-5	1.8011061853858784E-5	0.1360938347504887	2.251382731732348E-5
78-79	0.0020082333967052545	5.448346210792283E-4	3.602212370771757E-5	1.8011061853858784E-5	0.13752346278513874	2.7016592780788177E-5
80-81	0.002012736162168719	5.538401520061577E-4	4.052488917118227E-5	1.8011061853858784E-5	0.13882476200408006	2.9267975512520524E-5
82-83	0.0020307472240225777	5.62845682933087E-4	4.277627190291461E-5	2.251382731732348E-5	0.14024538450780316	3.3770740975985224E-5
84-85	0.0020487582858764366	5.673484483965517E-4	4.727903736637931E-5	2.251382731732348E-5	0.14171103466616092	3.602212370771757E-5
86-87	0.0020712721131937602	5.786053620552134E-4	4.9530420098111654E-5	2.251382731732348E-5	0.1433725551221794	3.602212370771757E-5
88-89	0.002087031792315887	5.853595102504105E-4	4.9530420098111654E-5	2.251382731732348E-5	0.14504533249185653	3.602212370771757E-5
90-91	0.002093785940511084	6.281357821533251E-4	4.9530420098111654E-5	2.7016592780788177E-5	0.1459841590909889	3.602212370771757E-5
92-93	0.0020960373232428164	6.303871648850575E-4	4.9530420098111654E-5	2.7016592780788177E-5	0.1468757066527549	3.602212370771757E-5
94-95	0.00210954561963321	6.326385476167898E-4	4.9530420098111654E-5	2.7016592780788177E-5	0.14782353878281423	4.277627190291461E-5
96-97	0.002120802533291872	6.438954612754516E-4	4.9530420098111654E-5	2.7016592780788177E-5	0.14890195111131405	4.727903736637931E-5
98-99	0.0021253052987553365	6.529009922023809E-4	4.9530420098111654E-5	2.7016592780788177E-5	0.14994434131610612	5.4033185561576354E-5
100-101	0.0021320594469505334	6.529009922023809E-4	4.9530420098111654E-5	2.7016592780788177E-5	0.15085615132245772	6.52900992202381E-5
102-103	0.002134310829682266	6.529009922023809E-4	4.9530420098111654E-5	2.7016592780788177E-5	0.15176796132880932	7.204424741543513E-5
104-105	0.0021388135951457307	6.529009922023809E-4	5.1781802829844004E-5	2.7016592780788177E-5	0.1526302409150628	7.204424741543513E-5
106-107	0.002179338484316913	6.619065231293104E-4	5.4033185561576354E-5	2.7016592780788177E-5	0.15335968892014407	7.654701287889983E-5
108-109	0.0021838412497803775	6.866717331783661E-4	5.4033185561576354E-5	2.7016592780788177E-5	0.15405986894971285	7.654701287889983E-5
110-111	0.0021838412497803775	7.001800295687602E-4	6.07873337567734E-5	2.7016592780788177E-5	0.1549063888568442	7.654701287889983E-5
112-113	0.0021838412497803775	7.091855604956897E-4	6.303871648850575E-5	2.7016592780788177E-5	0.15562683133099856	7.879839561063218E-5
114-115	0.0021883440152438423	7.11436943227422E-4	6.303871648850575E-5	2.7016592780788177E-5	0.15644183187988567	8.330116107409688E-5
116-117	0.0021973495461707717	7.136883259591543E-4	6.754148195197045E-5	2.7016592780788177E-5	0.15738741262721326	9.230669200102627E-5
118-119	0.002204103694365969	7.226938568860837E-4	6.754148195197045E-5	2.7016592780788177E-5	0.15816639105239266	9.680945746449096E-5
120-121	0.0022086064598294333	7.294480050812807E-4	6.754148195197045E-5	2.9267975512520524E-5	0.1589768888358163	1.0356360565968801E-4
122-123	0.0022153606080246306	7.294480050812807E-4	6.754148195197045E-5	3.1519358244252874E-5	0.15969958269270237	1.0806637112315271E-4
124-125	0.0022153606080246306	7.294480050812807E-4	7.204424741543513E-5	3.1519358244252874E-5	0.16055285674802894	1.1482051931834976E-4
126-127	0.002228868904415025	7.339507705447454E-4	7.654701287889983E-5	3.1519358244252874E-5	0.16147367228530746	1.215746675135468E-4
128-129	0.002240125818073686	7.407049187399425E-4	7.654701287889983E-5	3.1519358244252874E-5	0.16243726409448891	1.260774329770115E-4
130-131	0.002264891028122742	7.519618323986042E-4	8.104977834236452E-5	3.1519358244252874E-5	0.16335807963176746	1.350829639039409E-4
132-133	0.0022851534727083333	7.56464597862069E-4	8.555254380582922E-5	3.1519358244252874E-5	0.16413705805694684	1.4408849483087027E-4
134-135	0.0023144214482208538	7.609673633255336E-4	8.555254380582922E-5	3.1519358244252874E-5	0.16491828786485796	1.4408849483087027E-4
136-137	0.002323426979147783	7.654701287889983E-4	8.555254380582922E-5	3.1519358244252874E-5	0.16580082989569705	1.4408849483087027E-4
138-139	0.002327929744611248	7.744756597159277E-4	8.555254380582922E-5	3.1519358244252874E-5	0.16668787469199958	1.4859126029433497E-4
>>END_MODULE
