##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.4d.R.3.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22697696
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.047906976990085	37.0	35.0	38.0	33.0	39.0
2	35.50044480285576	35.0	34.0	38.0	33.0	39.0
3	35.647385708223425	36.0	35.0	38.0	33.0	39.0
4	35.73144917440079	36.0	35.0	38.0	33.0	39.0
5	35.65534043631565	36.0	35.0	38.0	33.0	39.0
6	35.58328995154398	36.0	35.0	37.0	33.0	39.0
7	35.54523507584206	35.0	34.0	38.0	33.0	39.0
8	35.7159549586002	36.0	35.0	38.0	33.0	39.0
9	35.68882383480685	36.0	35.0	38.0	33.0	39.0
10-14	35.613222311198456	36.0	35.0	37.8	33.0	39.0
15-19	35.61914969695603	36.0	35.0	38.0	33.0	39.0
20-24	35.60106195800667	36.0	35.0	38.0	33.0	39.0
25-29	35.583148694916	36.0	35.0	38.0	33.0	39.0
30-34	35.556987149708945	36.0	35.0	38.0	33.0	39.0
35-39	35.53172264709158	36.0	35.0	38.0	33.0	39.0
40-44	35.51291466763851	36.0	35.0	38.0	33.0	39.0
45-49	35.478877010248084	36.0	34.2	38.0	33.0	39.0
50-54	35.45497068953607	36.0	34.0	38.0	33.0	39.0
55-59	35.42314814684274	36.0	34.0	38.0	33.0	39.0
60-64	35.38237257208838	36.0	34.0	38.0	32.8	39.0
65-69	35.34320646465615	36.0	34.0	38.0	32.0	39.0
70-74	35.310372867801206	36.0	34.0	38.0	32.0	39.0
75-79	35.26219944085955	36.0	34.0	38.0	32.0	39.0
80-84	35.220211989798436	36.0	34.0	38.0	32.0	39.0
85-89	35.172129937769895	36.0	34.0	38.0	32.0	39.0
90-94	35.11251423933073	36.0	34.0	38.0	32.0	39.0
95-99	35.0447018587261	35.8	34.0	37.2	32.0	39.0
100-104	34.9910691904588	35.6	34.0	37.0	31.6	39.0
105-109	34.9202359746117	35.0	34.0	37.0	31.0	39.0
110-114	34.85282107047341	35.0	34.0	37.0	31.0	39.0
115-119	34.77581317504649	35.0	34.0	37.0	31.0	39.0
120-124	34.695912889132	35.0	34.0	37.0	30.6	39.0
125-129	34.62691229100962	35.0	34.0	37.0	30.0	39.0
130-134	34.52894597760054	35.0	34.0	37.0	30.0	39.0
135-139	34.42936598498808	35.0	34.0	37.0	30.0	39.0
140-144	34.321675424677466	35.0	34.0	37.0	29.8	39.0
145-149	34.22671866783307	35.0	34.0	37.0	29.0	39.0
150	34.15283718664661	35.0	34.0	37.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	15.0
21	261.0
22	1994.0
23	9507.0
24	30663.0
25	72560.0
26	136650.0
27	225732.0
28	320379.0
29	414650.0
30	526821.0
31	686856.0
32	930140.0
33	1334770.0
34	2101229.0
35	3857411.0
36	1.0530442E7
37	1517608.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.39411647772532	18.125214118648874	16.588740989393813	30.891928414232
2	23.91623907185289	25.136636120980565	33.06904952242948	17.878075284737065
3	20.34197656008786	29.13703664019467	30.11047464905689	20.41051215066058
4	23.69741949568007	33.743082722717	22.370187809819456	20.18930997178348
5	25.157544624793637	35.07631787825513	22.68351378042952	17.08262371652171
6	19.012652209281505	39.10957746548372	22.996201905250647	18.881568419984127
7	20.413619092158918	20.679487498620457	38.29518371799428	20.611709691226356
8	21.77940439417287	23.246456380418522	28.669821818038272	26.304317407370338
9	23.769672481295018	22.963088412145442	29.028875882380305	24.23836322417923
10-14	23.22109641054709	29.11760109882423	25.876636219750836	21.78466627087784
15-19	23.053467629489795	28.256051186869364	27.249515545542597	21.440965638098245
20-24	23.081493381530883	28.356958345023216	27.08675629455959	21.474791978886316
25-29	23.200830808561633	28.313044886256694	26.997779219510026	21.488345085671646
30-34	23.192681759417344	28.093133329479787	27.176223525066156	21.537961386036713
35-39	23.264280215930285	28.103146680614632	27.126900457209402	21.50567264624568
40-44	23.405404878146268	27.865641762632137	27.238719095001706	21.490234264219897
45-49	23.403207973179306	27.702580913939457	27.327339303513448	21.56687180936779
50-54	23.457157267919683	27.668457870512125	27.290083780222307	21.584301081345885
55-59	23.615403078797073	27.5514193158636	27.199020552570623	21.634157052768703
60-64	23.51546958775023	27.531250748974696	27.28376659904159	21.66951306423348
65-69	23.60442310972885	27.444959171186362	27.255938223862014	21.694679495222776
70-74	23.693920299223322	27.396652946625068	27.180371963744694	21.72905479040692
75-79	23.610143257099132	27.428167883511207	27.26248586453407	21.699202994855593
80-84	23.725982793372356	27.471919858931233	27.12065424720337	21.68144310049305
85-89	23.733296311073126	27.42545258713001	27.183224475146943	21.658026626649917
90-94	23.68021582454889	27.371088237325935	27.234345723900788	21.71435021422439
95-99	23.73668868138373	27.456951025203217	27.255423953842012	21.550936339571045
100-104	23.910298418925855	27.410060716383377	27.073472365419576	21.60616849927119
105-109	23.692931652622363	27.53837305777644	27.180049464051333	21.58864582554987
110-114	23.71897923031483	27.53208167031579	27.117614933251378	21.631324166118006
115-119	23.785419663611844	27.524527842160968	27.14943514222267	21.540617352004514
120-124	23.74555725832261	27.44479527789957	27.308140879144737	21.501506584633084
125-129	23.71802691971931	27.542982578874813	27.263878477039068	21.47511202436681
130-134	23.790410268954172	27.451938734222185	27.317251936055538	21.440399060768105
135-139	23.689048439101484	27.447943615070002	27.4259686974396	21.437039248388913
140-144	23.706549774919885	27.596188617558365	27.371659220389592	21.325602387132157
145-149	23.835877648866894	28.297430966538904	26.731821826601447	21.134869557992754
150	23.43759912900411	28.188041640878442	26.872044633957557	21.50231459615989
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.5
2	13.0
3	31.5
4	47.0
5	48.5
6	42.5
7	36.5
8	29.5
9	28.0
10	20.0
11	17.0
12	21.0
13	15.5
14	19.0
15	42.5
16	68.0
17	106.5
18	194.0
19	360.0
20	561.0
21	920.0
22	1474.5
23	2427.5
24	4037.5
25	6604.5
26	10142.0
27	15882.0
28	26114.5
29	39957.0
30	55126.0
31	73373.5
32	99007.0
33	133023.5
34	177151.5
35	241892.5
36	338205.5
37	455154.0
38	591798.5
39	765807.0
40	969890.5
41	1160979.5
42	1331513.5
43	1490551.5
44	1605263.5
45	1653709.5
46	1643742.0
47	1584138.5
48	1484013.5
49	1344527.5
50	1164369.0
51	972593.0
52	790854.0
53	628068.0
54	488912.5
55	367467.0
56	270361.0
57	200773.0
58	147781.5
59	106670.0
60	74285.0
61	49855.5
62	34567.0
63	24911.0
64	17526.0
65	12762.5
66	9631.5
67	7185.5
68	6416.5
69	5044.0
70	2946.5
71	2112.0
72	1549.5
73	1176.0
74	940.5
75	521.0
76	190.0
77	70.5
78	20.5
79	2.0
80	1.5
81	1.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.4057335158599355E-6
3	0.0
4	4.4057335158599355E-6
5	0.0
6	0.0
7	4.4057335158599355E-6
8	0.0
9	0.0
10-14	0.09678251043630155
15-19	0.0
20-24	0.0
25-29	8.811467031719872E-7
30-34	0.0
35-39	0.0
40-44	8.811467031719872E-7
45-49	0.0
50-54	8.811467031719872E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	2.6434401095159613E-6
80-84	8.811467031719872E-7
85-89	8.811467031719872E-7
90-94	0.0
95-99	4.4057335158599355E-6
100-104	8.811467031719872E-7
105-109	0.0
110-114	0.0
115-119	8.811467031719872E-7
120-124	0.0
125-129	8.811467031719872E-7
130-134	0.0
135-139	0.0
140-144	0.0
145-149	8.811467031719872E-7
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.2697696E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.258509058537435
#Duplication Level	Percentage of total
1	26.06594936820229
2	11.492292772619404
3	7.562312448560552
4	5.324880816683243
5	4.108458328697054
6	3.2933830605536905
7	2.6880566075868724
8	2.3199072926004716
9	1.9625153840100131
>10	22.05353627108467
>50	5.538423841702334
>100	5.965553005969009
>500	0.8564640972090621
>1k	0.687832567637751
>5k	0.08043413688337478
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	4.4057335158599355E-6	0.0	0.0	0.0017006131371219352	0.0
2	8.811467031719871E-6	2.2028667579299678E-5	0.0	0.0	0.005414646490991861	0.0
3	5.7274535706179165E-5	2.6434401095159613E-5	0.0	0.0	0.007066796559439337	0.0
4	1.1454907141235833E-4	3.084013461101955E-5	0.0	0.0	0.008141795537309161	0.0
5	1.1454907141235833E-4	3.084013461101955E-5	0.0	0.0	0.00964855639973326	0.0
6	1.233605384440782E-4	3.084013461101955E-5	0.0	0.0	0.011172940196220798	0.0
7	1.3657773899165802E-4	6.16802692220391E-5	0.0	0.0	0.013146708811326048	0.0
8	1.718236071185375E-4	7.049173625375897E-5	0.0	0.0	0.017918118209002357	0.0
9	1.8063507415025737E-4	7.930320328547885E-5	0.0	0.0	0.023645571779620275	0.0
10-11	1.9825800821369712E-4	8.370893680133878E-5	0.0	0.0	0.02939064828430163	0.0
12-13	2.4011247661436647E-4	8.370893680133878E-5	0.0	0.0	0.03481630910908314	0.0
14-15	2.9738701232054566E-4	8.370893680133878E-5	0.0	0.0	0.04230825895280296	0.0
16-17	3.5025581451086484E-4	8.811467031719871E-5	2.2028667579299678E-6	0.0	0.047716296843521036	0.0
18-19	4.2955901779634376E-4	1.035347376227085E-4	4.4057335158599355E-6	0.0	0.05509149474907057	0.0
20-21	4.934421537763128E-4	1.1234620465442837E-4	4.4057335158599355E-6	0.0	0.06031889756563838	0.0
22-23	5.573252897562819E-4	1.2776627195993813E-4	1.3217200547579807E-5	0.0	0.06577539852503092	0.0
24-25	6.079912251886711E-4	1.4098347250751794E-4	1.3217200547579807E-5	0.0	0.07091909240479738	0.0
26-27	6.498456935893405E-4	1.4538920602337788E-4	1.3217200547579807E-5	0.0	0.07483578950039688	0.0
28-29	7.665976317596288E-4	1.4538920602337788E-4	1.3217200547579807E-5	0.0	0.078763500929786	0.0
30-31	9.780728405209057E-4	1.4979493953923783E-4	1.3217200547579807E-5	0.0	0.08351288165988302	0.0
32-33	0.0011565050479132332	1.6741787360267756E-4	1.3217200547579807E-5	0.0	0.08803756998067117	0.0
34-35	0.0013283286550317707	1.718236071185375E-4	1.3217200547579807E-5	0.0	0.09073387889237744	0.0
36-37	0.0013922117910117396	1.718236071185375E-4	1.7622934063439742E-5	0.0	0.09405580196333585	0.0
38-39	0.001445080593202059	1.718236071185375E-4	2.2028667579299678E-5	0.0	0.09721471289420741	0.0
40-41	0.0014627035272654987	1.7402647387646745E-4	2.2028667579299678E-5	0.0	0.09980748706829098	0.0
42-43	0.0014803264613289383	1.8283794090818734E-4	2.2028667579299678E-5	0.0	0.1027262855225482	0.0
44-45	0.001513369462697888	1.850408076661173E-4	2.2028667579299678E-5	0.0	0.10505471568568017	0.0
46-47	0.0015486153308247675	2.3130100958264663E-4	2.2028667579299678E-5	0.0	0.1075417522553831	4.4057335158599355E-6
48-49	0.0016058898665309465	2.5553254391987625E-4	2.2028667579299678E-5	0.0	0.10997812288965364	4.4057335158599355E-6
50-51	0.0016675701357529856	2.599382774357362E-4	2.2028667579299678E-5	0.0	0.11198273163936992	4.4057335158599355E-6
52-53	0.0017116274709115852	2.6654687770952615E-4	2.2028667579299678E-5	0.0	0.11406664359237167	1.1014333789649839E-5
54-55	0.0017666991398598343	2.7976407825710593E-4	2.2028667579299678E-5	0.0	0.1158840086676639	1.3217200547579807E-5
56-57	0.0018415966096294533	2.929812788046857E-4	2.2028667579299678E-5	0.0	0.11779169128003125	1.7622934063439742E-5
58-59	0.0018988711453356323	2.973870123205457E-4	2.2028667579299678E-5	0.0	0.11968395382509309	1.7622934063439742E-5
60-61	0.0019539428142838814	3.106042128681255E-4	2.2028667579299678E-5	0.0	0.12098805094578763	1.7622934063439742E-5
62-63	0.0019781743486211112	3.260242801736352E-4	2.2028667579299678E-5	0.0	0.12288031349084946	1.7622934063439742E-5
64-65	0.0020024058829583406	3.3703861396328506E-4	2.2028667579299678E-5	4.4057335158599355E-6	0.12464480976395137	1.7622934063439742E-5
66-67	0.002116954954370699	3.6127014830051473E-4	2.2028667579299678E-5	4.4057335158599355E-6	0.12597534128574106	1.7622934063439742E-5
68-69	0.0021522008224975787	3.678787485743046E-4	2.2028667579299678E-5	4.4057335158599355E-6	0.1273653502099949	1.7622934063439742E-5
70-71	0.002178635223592738	3.744873488480945E-4	2.2028667579299678E-5	4.4057335158599355E-6	0.12866063586365772	2.2028667579299678E-5
72-73	0.002205069624687898	3.832988158798144E-4	2.2028667579299678E-5	6.608600273789903E-6	0.1295351739665559	3.084013461101955E-5
74-75	0.002220489691993408	3.9431314966946424E-4	2.8637267853089583E-5	8.811467031719871E-6	0.13058814427684642	3.084013461101955E-5
76-77	0.002257938426878217	4.2955901779634376E-4	3.304300136894952E-5	8.811467031719871E-6	0.13172262065718038	3.084013461101955E-5
78-79	0.0023174158293423263	4.3837048482806365E-4	3.5245868126879484E-5	8.811467031719871E-6	0.13314567258280313	3.084013461101955E-5
80-81	0.002339444496921626	4.537905521335734E-4	3.744873488480946E-5	1.1014333789649839E-5	0.13452907290678315	3.084013461101955E-5
82-83	0.0023482559639533456	4.78022086470803E-4	4.405733515859936E-5	1.3217200547579807E-5	0.13611073123897685	3.084013461101955E-5
84-85	0.0023702846315326455	4.80224953228733E-4	4.8463068674459294E-5	1.7622934063439742E-5	0.1376130863678851	3.084013461101955E-5
86-87	0.002398921899385735	4.890364202604529E-4	4.8463068674459294E-5	1.7622934063439742E-5	0.13941062564235596	3.304300136894952E-5
88-89	0.0024231534337229646	5.000507540501027E-4	4.8463068674459294E-5	1.7622934063439742E-5	0.14106277571080342	3.744873488480946E-5
90-91	0.0024341677675126143	5.066593543238926E-4	4.8463068674459294E-5	1.7622934063439742E-5	0.1420738915526933	3.965160164273942E-5
92-93	0.002440776367786404	5.110650878397525E-4	4.8463068674459294E-5	1.7622934063439742E-5	0.14303654432590868	3.965160164273942E-5
94-95	0.0024495878348181244	5.198765548714724E-4	5.2868802190319226E-5	1.7622934063439742E-5	0.14410934043702056	4.185446840066939E-5
96-97	0.002460602168607774	5.242822883873323E-4	5.2868802190319226E-5	1.7622934063439742E-5	0.1450169215412877	4.8463068674459294E-5
98-99	0.0024760222359132837	5.330937554190523E-4	5.2868802190319226E-5	1.9825800821369708E-5	0.1459355169793445	5.066593543238926E-5
100-101	0.0024804279694291436	5.374994889349122E-4	5.2868802190319226E-5	2.2028667579299678E-5	0.14683428661657993	5.50716689482492E-5
102-103	0.002484833702945004	5.46310955966632E-4	5.2868802190319226E-5	2.2028667579299678E-5	0.14770882471947813	5.947740246410913E-5
104-105	0.002484833702945004	5.573252897562819E-4	5.50716689482492E-5	2.2028667579299678E-5	0.14852829115342808	6.16802692220391E-5
106-107	0.0025068623705243034	5.727453570617916E-4	5.7274535706179165E-5	2.2028667579299678E-5	0.14931030885249325	6.16802692220391E-5
108-109	0.002515673837556023	5.859625576093714E-4	5.7274535706179165E-5	2.2028667579299678E-5	0.15011876095265353	6.608600273789904E-5
110-111	0.0025288910381036033	5.969768913990214E-4	5.7274535706179165E-5	2.2028667579299678E-5	0.15109022519290063	7.049173625375897E-5
112-113	0.002535499638377393	5.991797581569513E-4	6.16802692220391E-5	2.2028667579299678E-5	0.15187885149223956	7.049173625375897E-5
114-115	0.002539905371893253	6.013826249148813E-4	6.608600273789904E-5	2.2028667579299678E-5	0.15282828706490736	7.489746976961891E-5
116-117	0.002542108238651183	6.057883584307412E-4	6.8288869495829E-5	2.2028667579299678E-5	0.15389447457574548	8.15060700434088E-5
118-119	0.0025509197056829028	6.079912251886711E-4	7.049173625375897E-5	2.2028667579299678E-5	0.15467869514156857	8.370893680133878E-5
120-121	0.0025575283059566926	6.079912251886711E-4	7.049173625375897E-5	2.2028667579299678E-5	0.15554662464419294	9.252040383305866E-5
122-123	0.0025597311727146225	6.10194091946601E-4	7.049173625375897E-5	2.2028667579299678E-5	0.15635948247786913	9.692613734891859E-5
124-125	0.0025597311727146225	6.168026922203911E-4	7.489746976961891E-5	2.2028667579299678E-5	0.1571943689791246	1.0573760438063845E-4
126-127	0.0025707455065042727	6.432370933155507E-4	7.489746976961891E-5	2.2028667579299678E-5	0.15814600741855034	1.0573760438063845E-4
128-129	0.002590571307325642	6.498456935893405E-4	7.489746976961891E-5	2.2028667579299678E-5	0.15908222579067055	1.101433378964984E-4
130-131	0.002597179907599432	6.564542938631304E-4	7.489746976961891E-5	2.2028667579299678E-5	0.1601285875006873	1.1454907141235833E-4
132-133	0.002634628642484242	6.608600273789903E-4	7.489746976961891E-5	2.6434401095159613E-5	0.16095686540166898	1.1895480492821826E-4
134-135	0.002667671643853191	6.608600273789903E-4	7.489746976961891E-5	2.6434401095159613E-5	0.1617388831007341	1.2115767168614824E-4
136-137	0.002676483110884911	6.740772279265701E-4	7.710033652754889E-5	2.6434401095159613E-5	0.1626090154701164	1.233605384440782E-4
138-139	0.0026874974446745607	6.8729442847415E-4	7.930320328547885E-5	2.8637267853089583E-5	0.16335799016781263	1.3437487223372805E-4
>>END_MODULE
