##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.4d.S.1.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24030269
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.97378618608057	37.0	35.0	38.0	33.0	39.0
2	35.459370180167355	35.0	34.0	38.0	33.0	39.0
3	35.59291999602668	36.0	35.0	37.0	33.0	39.0
4	35.67777114771374	36.0	35.0	38.0	33.0	39.0
5	35.610179727909	36.0	35.0	38.0	33.0	39.0
6	35.541082915051845	36.0	35.0	37.0	33.0	39.0
7	35.5185655225083	35.0	34.0	38.0	33.0	39.0
8	35.65264874895907	36.0	35.0	38.0	33.0	39.0
9	35.64106406798859	36.0	35.0	38.0	33.0	39.0
10-14	35.559106650033755	36.0	35.0	37.8	33.0	39.0
15-19	35.56763402856622	36.0	35.0	38.0	33.0	39.0
20-24	35.55095550532539	36.0	35.0	38.0	33.0	39.0
25-29	35.53072662648928	36.0	35.0	38.0	33.0	39.0
30-34	35.50550020060116	36.0	34.4	38.0	33.0	39.0
35-39	35.47983472011903	36.0	34.0	38.0	33.0	39.0
40-44	35.460692104611894	36.0	34.0	38.0	33.0	39.0
45-49	35.421322890725854	36.0	34.0	38.0	33.0	39.0
50-54	35.402883180375554	36.0	34.0	38.0	32.6	39.0
55-59	35.363983957066814	36.0	34.0	38.0	32.0	39.0
60-64	35.323468014444614	36.0	34.0	38.0	32.0	39.0
65-69	35.281688190839645	36.0	34.0	38.0	32.0	39.0
70-74	35.24654021143084	36.0	34.0	38.0	32.0	39.0
75-79	35.19314137515481	36.0	34.0	38.0	32.0	39.0
80-84	35.14795965038927	36.0	34.0	38.0	32.0	39.0
85-89	35.10026593543334	36.0	34.0	38.0	32.0	39.0
90-94	35.04032003137377	36.0	34.0	37.6	32.0	39.0
95-99	34.96787795425844	35.2	34.0	37.2	31.0	39.0
100-104	34.909101483632995	35.0	34.0	37.0	31.0	39.0
105-109	34.83926275648433	35.0	34.0	37.0	31.0	39.0
110-114	34.75919750211701	35.0	34.0	37.0	31.0	39.0
115-119	34.678506794909374	35.0	34.0	37.0	30.2	39.0
120-124	34.591655823744624	35.0	34.0	37.0	30.0	39.0
125-129	34.52198696568898	35.0	34.0	37.0	30.0	39.0
130-134	34.42004192295975	35.0	34.0	37.0	30.0	39.0
135-139	34.316492553620606	35.0	34.0	37.0	29.2	39.0
140-144	34.20947699753174	35.0	34.0	37.0	29.0	39.0
145-149	34.106289471832376	35.0	34.0	37.0	28.8	39.0
150	34.037697330812236	35.0	34.0	37.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	16.0
21	257.0
22	2025.0
23	9872.0
24	32878.0
25	79330.0
26	150903.0
27	251196.0
28	358016.0
29	463384.0
30	593708.0
31	779167.0
32	1060794.0
33	1514995.0
34	2350972.0
35	4148487.0
36	1.0337519E7
37	1896743.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.46539316725918	19.20240260315022	15.345529423744694	31.98667480584591
2	24.40489219679115	25.95168310232745	32.05725795484262	17.586166746038785
3	20.204438826714757	29.462599856872178	29.761988931542966	20.5709723848701
4	23.57160144863969	34.00091085126475	21.898174419028535	20.529313281067026
5	25.645556678768628	34.94509507759131	21.93469918854005	17.47464905510001
6	19.34503105229492	39.316871567272095	22.02328238606068	19.314814994372306
7	20.596573429952034	21.18274664341044	36.955379067958	21.265300858679524
8	21.612920770882756	23.90919552336264	28.09267345280238	26.385210252952223
9	23.596868599348596	23.47706969073047	28.050909459232436	24.875152250688494
10-14	23.085397176756178	29.18391576859359	25.29115430926774	22.439532745382493
15-19	23.19929809855973	27.96227860692559	26.795763130136326	22.042660164378354
20-24	23.066714925802245	28.00230349483232	26.718334503191244	22.212647076174196
25-29	23.206159891145287	27.6303051591333	26.69457363706227	22.46896131265914
30-34	23.06230529504268	27.326845155166595	26.907390008825953	22.703459540964772
35-39	23.349889258418205	27.064870559709508	26.909262647039036	22.675977534833255
40-44	23.50598156017313	26.726179386506242	26.979937677767985	22.78790137555264
45-49	23.42281267717499	26.57831048962973	27.091878615122926	22.906998218072353
50-54	23.573714028315106	26.548530547844145	26.878822319946938	22.998933103893812
55-59	23.666165368352722	26.490624803242945	26.91128592859281	22.931923899811526
60-64	23.42095981880985	26.44510326909036	26.98048471268037	23.153452199419426
65-69	23.574669929828918	26.39987675543707	26.92226042080511	23.103192893928902
70-74	23.786728313361785	26.336004811265322	26.70928818982426	23.16797868554863
75-79	23.639260528282236	26.46543368225189	26.904291864636086	22.991013924829787
80-84	23.910777061303616	26.439129136999085	26.621391767369662	23.02870203432764
85-89	23.733790079503482	26.51888166545285	26.775262482496554	22.972065772547115
90-94	23.69858864251582	26.338966908776595	26.78179840600203	23.180646042705554
95-99	23.709328147473478	26.553679327316352	26.821155333284764	22.915837191925405
100-104	23.95354102661625	26.289854506993354	26.783928888617826	22.972675577772574
105-109	23.76034325708131	26.48060826951209	26.75614659161743	23.00290188178917
110-114	23.859792196748128	26.585545310254705	26.645761865135693	22.908900627861474
115-119	23.969130965359824	26.594746206084885	26.783408265495556	22.652714563059735
120-124	23.86228385541585	26.677710515849824	26.97766054970088	22.482345079033447
125-129	23.89268904058202	26.776829895469167	26.791691984735515	22.538789079213295
130-134	24.061507592778092	26.771139349293176	26.73313644553875	22.43421661238998
135-139	23.879601181326766	26.840385348994637	26.918361171903655	22.361652297774945
140-144	23.88499457817134	26.973679961499226	26.790443559249745	22.350881901079692
145-149	24.030656702433557	27.719386143529114	26.125017794224593	22.124939359812736
150	23.814822880259893	27.661325805383203	26.12445578532642	22.399395529030492
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	4.0
2	12.5
3	28.5
4	50.5
5	61.0
6	55.0
7	45.5
8	33.5
9	25.0
10	23.0
11	20.0
12	17.5
13	25.0
14	30.0
15	38.0
16	64.5
17	119.5
18	225.5
19	388.0
20	627.0
21	994.0
22	1446.0
23	2237.5
24	3708.0
25	5830.5
26	9010.5
27	13898.5
28	20467.0
29	28911.0
30	40515.5
31	57702.0
32	83900.0
33	114281.5
34	150347.0
35	209257.5
36	295510.0
37	393263.0
38	508823.5
39	661750.0
40	843714.0
41	1033572.5
42	1224438.0
43	1400974.0
44	1533125.5
45	1613149.5
46	1634733.0
47	1587483.5
48	1518965.0
49	1456256.5
50	1342337.0
51	1176139.5
52	1001078.5
53	833619.5
54	682165.5
55	564153.5
56	465416.5
57	383153.0
58	321105.5
59	261804.5
60	198753.0
61	136217.5
62	85437.0
63	51883.0
64	31731.5
65	16951.0
66	10126.5
67	5705.0
68	3265.5
69	2172.0
70	1606.0
71	1403.5
72	1243.0
73	1043.0
74	851.0
75	512.5
76	156.5
77	53.0
78	12.5
79	4.5
80	4.5
81	3.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.161418251289655E-6
3	0.0
4	4.161418251289655E-6
5	4.161418251289655E-6
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.10628678355618906
15-19	2.496850950773793E-6
20-24	8.32283650257931E-7
25-29	8.32283650257931E-7
30-34	0.0
35-39	0.0
40-44	0.0
45-49	1.664567300515862E-6
50-54	1.664567300515862E-6
55-59	0.0
60-64	8.32283650257931E-7
65-69	0.0
70-74	0.0
75-79	1.664567300515862E-6
80-84	8.32283650257931E-7
85-89	0.0
90-94	0.0
95-99	5.825985551805517E-6
100-104	2.496850950773793E-6
105-109	0.0
110-114	8.32283650257931E-7
115-119	8.32283650257931E-7
120-124	0.0
125-129	8.32283650257931E-7
130-134	0.0
135-139	0.0
140-144	8.32283650257931E-7
145-149	8.32283650257931E-7
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4030269E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.19809231749293
#Duplication Level	Percentage of total
1	22.562027943507896
2	9.465164541393376
3	5.985000532022646
4	4.231229129391664
5	3.0998362499706036
6	2.4549950744221554
7	2.018644005623443
8	1.7940822776010719
9	1.4997851716594037
>10	18.272158697420142
>50	6.512358491961582
>100	13.080920459539957
>500	3.6328042075731606
>1k	4.447339447278233
>5k	0.7013943708453114
>10k+	0.24225939978923458
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.663307680825379E-4	0.0	0.0	0.0	0.0031044180154620824	0.0
2	0.0013899136959307447	4.161418251289655E-6	0.0	0.0	0.012363573624581565	0.0
3	0.0020515791978857997	4.161418251289655E-6	0.0	0.0	0.0166123816591483	0.0
4	0.005168481468101751	4.161418251289655E-6	0.0	0.0	0.02253407983073348	0.0
5	0.005389036635420103	8.32283650257931E-6	0.0	4.161418251289655E-6	0.027074187142890494	0.0
6	0.006654107783812158	8.32283650257931E-6	0.0	8.32283650257931E-6	0.03394468867576971	0.0
7	0.008714009818200537	1.2484254753868965E-5	0.0	8.32283650257931E-6	0.044527175288799306	0.0
8	0.015467991640043647	1.664567300515862E-5	4.161418251289655E-6	8.32283650257931E-6	0.06029478904293581	0.0
9	0.016083881541234517	2.496850950773793E-5	4.161418251289655E-6	8.32283650257931E-6	0.07223389800588582	0.0
10-11	0.016660237969038132	2.496850950773793E-5	4.161418251289655E-6	8.32283650257931E-6	0.12145931450039114	0.0
12-13	0.01726156290634949	2.704921863338276E-5	4.161418251289655E-6	8.32283650257931E-6	0.1479509030881011	0.0
14-15	0.019689750455977	3.329134601031724E-5	4.161418251289655E-6	8.32283650257931E-6	0.15987752779629724	0.0
16-17	0.02249038493909494	3.329134601031724E-5	4.161418251289655E-6	8.32283650257931E-6	0.16799021267718642	0.0
18-19	0.028410002401554474	3.329134601031724E-5	4.161418251289655E-6	8.32283650257931E-6	0.17592395657327015	0.0
20-21	0.03379903903697458	3.7452764261606895E-5	4.161418251289655E-6	8.32283650257931E-6	0.18157724326764715	0.0
22-23	0.03601291354666067	4.1614182512896545E-5	4.161418251289655E-6	8.32283650257931E-6	0.1869309078479313	0.0
24-25	0.04062376496908961	4.1614182512896545E-5	4.161418251289655E-6	8.32283650257931E-6	0.19251761185028765	0.0
26-27	0.044660340672840575	4.993701901547586E-5	4.161418251289655E-6	8.32283650257931E-6	0.19699737859780098	0.0
28-29	0.05395278762797037	4.993701901547586E-5	4.161418251289655E-6	1.0403545628224136E-5	0.20075721998784116	0.0
30-31	0.07519682780080406	5.409843726676551E-5	8.32283650257931E-6	1.2484254753868965E-5	0.20511630560606708	0.0
32-33	0.0951945232073765	5.409843726676551E-5	8.32283650257931E-6	1.664567300515862E-5	0.2089843438706408	0.0
34-35	0.11089971568774365	5.617914639241034E-5	8.32283650257931E-6	2.0807091256448273E-5	0.21257356711237815	4.161418251289655E-6
36-37	0.11372115726211804	6.0340564643699996E-5	8.32283650257931E-6	2.496850950773793E-5	0.21473542389392314	4.161418251289655E-6
38-39	0.11640527203419987	6.450198289498964E-5	8.32283650257931E-6	2.496850950773793E-5	0.21674122749104474	4.161418251289655E-6
40-41	0.11801574089744896	7.074411027192413E-5	8.32283650257931E-6	2.9129927759027584E-5	0.21856184797598394	4.161418251289655E-6
42-43	0.1183236858480444	7.906694677450345E-5	8.32283650257931E-6	2.9129927759027584E-5	0.22033877356928463	4.161418251289655E-6
44-45	0.11903736907814058	8.114765590014827E-5	8.32283650257931E-6	2.9129927759027584E-5	0.22183272272149762	6.2421273769344824E-6
46-47	0.12037942646418148	8.947049240272758E-5	8.32283650257931E-6	2.9129927759027584E-5	0.2233225104554593	8.32283650257931E-6
48-49	0.12157375350230162	9.571261977966206E-5	8.32283650257931E-6	2.9129927759027584E-5	0.2246999398966362	8.32283650257931E-6
50-51	0.12243100566206729	9.571261977966206E-5	8.32283650257931E-6	2.9129927759027584E-5	0.22608777288344128	8.32283650257931E-6
52-53	0.12325912789407392	9.987403803095172E-5	8.32283650257931E-6	2.9129927759027584E-5	0.22733411764970254	8.32283650257931E-6
54-55	0.12369815751958499	1.0403545628224136E-4	8.32283650257931E-6	2.9129927759027584E-5	0.22852844468782268	8.32283650257931E-6
56-57	0.1245346025880942	1.1860042016175517E-4	8.32283650257931E-6	2.9129927759027584E-5	0.22979559654534037	8.32283650257931E-6
58-59	0.12470522073639709	1.3108467491562413E-4	1.0403545628224136E-5	2.9129927759027584E-5	0.2309066952184347	8.32283650257931E-6
60-61	0.12496322866797704	1.3940751141820344E-4	1.2484254753868965E-5	2.9129927759027584E-5	0.23183677219759796	1.2484254753868965E-5
62-63	0.12523372085431086	1.456496387951379E-4	1.2484254753868965E-5	2.9129927759027584E-5	0.2328459161235357	1.664567300515862E-5
64-65	0.12526493149119555	1.518917661720724E-4	1.2484254753868965E-5	2.9129927759027584E-5	0.23380096161220668	1.664567300515862E-5
66-67	0.12532527205583924	1.6645673005158618E-4	1.2484254753868965E-5	2.9129927759027584E-5	0.23462908384421333	1.664567300515862E-5
68-69	0.12535856340184956	1.68537439177231E-4	1.2484254753868965E-5	2.9129927759027584E-5	0.23543847969408915	1.664567300515862E-5
70-71	0.1254709216946344	1.7477956655416552E-4	1.2484254753868965E-5	2.9129927759027584E-5	0.23629573185385483	1.664567300515862E-5
72-73	0.12552918155015244	1.7894098480545517E-4	1.2484254753868965E-5	2.9129927759027584E-5	0.23695115522843296	1.664567300515862E-5
74-75	0.12555206935053453	1.8518311218238963E-4	1.2484254753868965E-5	3.7452764261606895E-5	0.23778343887869086	1.664567300515862E-5
76-77	0.1255832799874192	1.9350594868496895E-4	1.2484254753868965E-5	3.7452764261606895E-5	0.238596996146818	1.664567300515862E-5
78-79	0.12562697487905775	2.0807091256448273E-4	1.2484254753868965E-5	3.7452764261606895E-5	0.2396061400727557	1.8726382130803444E-5
80-81	0.1256706697706963	2.1015162169012755E-4	1.4564963879513792E-5	3.7452764261606895E-5	0.2404488272686419	2.496850950773793E-5
82-83	0.12569771898932966	2.205551673183517E-4	1.664567300515862E-5	3.953347338725172E-5	0.24131440226491013	2.496850950773793E-5
84-85	0.12572892962621435	2.351201311978655E-4	1.664567300515862E-5	4.1614182512896545E-5	0.24228609342658627	3.121063688467241E-5
86-87	0.1257643016813503	2.3928154944915516E-4	2.0807091256448273E-5	4.1614182512896545E-5	0.2431870404779905	3.7452764261606895E-5
88-89	0.12578302806348113	2.4136225857479998E-4	2.0807091256448273E-5	4.1614182512896545E-5	0.24419202298567694	4.1614182512896545E-5
90-91	0.1258100772821145	2.538465133286689E-4	2.0807091256448273E-5	4.1614182512896545E-5	0.24491402905227572	4.1614182512896545E-5
92-93	0.125824642245994	2.580079315799586E-4	2.0807091256448273E-5	4.3694891638541374E-5	0.2455965016454872	4.1614182512896545E-5
94-95	0.1258620950102556	2.642500589568931E-4	2.0807091256448273E-5	5.201772814112068E-5	0.24628521636607564	4.1614182512896545E-5
96-97	0.12587457926500947	2.746536045851172E-4	2.28878003820931E-5	5.409843726676551E-5	0.24696768895928714	4.1614182512896545E-5
98-99	0.1258849828106377	2.746536045851172E-4	2.496850950773793E-5	5.409843726676551E-5	0.247554448932719	4.993701901547586E-5
100-101	0.1258995477745172	2.746536045851172E-4	2.496850950773793E-5	5.409843726676551E-5	0.24810167543276357	4.993701901547586E-5
102-103	0.1258995477745172	2.746536045851172E-4	2.496850950773793E-5	5.409843726676551E-5	0.248696758242698	5.409843726676551E-5
104-105	0.12593283912052755	2.746536045851172E-4	2.9129927759027584E-5	5.409843726676551E-5	0.24939379579978901	5.409843726676551E-5
106-107	0.1259598883391609	2.7673431371076206E-4	2.9129927759027584E-5	5.409843726676551E-5	0.24994310300895922	5.617914639241034E-5
108-109	0.12598485684866867	2.8713785933898617E-4	3.329134601031724E-5	5.409843726676551E-5	0.25047784525425	5.825985551805517E-5
110-111	0.1260514395406893	2.89218568464631E-4	3.329134601031724E-5	5.409843726676551E-5	0.25108125090068695	6.450198289498964E-5
112-113	0.12606600450456878	2.912992775902758E-4	3.329134601031724E-5	5.409843726676551E-5	0.25172002860225995	7.074411027192413E-5
114-115	0.1260888923049509	2.912992775902758E-4	3.329134601031724E-5	5.409843726676551E-5	0.25232343424869697	7.490552852321379E-5
116-117	0.12609721514145347	2.912992775902758E-4	3.329134601031724E-5	5.409843726676551E-5	0.2528727414578672	7.490552852321379E-5
118-119	0.1261034572688304	2.912992775902758E-4	3.7452764261606895E-5	5.409843726676551E-5	0.2534012415757809	7.906694677450345E-5
120-121	0.12610969939620736	2.912992775902758E-4	3.7452764261606895E-5	5.409843726676551E-5	0.2540212928952231	7.906694677450345E-5
122-123	0.12611386081445863	2.9546069584156546E-4	3.7452764261606895E-5	5.409843726676551E-5	0.25466631272417295	8.114765590014827E-5
124-125	0.1261221836509612	2.9546069584156546E-4	3.7452764261606895E-5	5.409843726676551E-5	0.25536543099038966	8.530907415143791E-5
126-127	0.12613050648746377	2.9546069584156546E-4	3.7452764261606895E-5	5.409843726676551E-5	0.2559896437280831	8.738978327708275E-5
128-129	0.1261346679057151	2.9962211409285516E-4	3.7452764261606895E-5	5.409843726676551E-5	0.25662842142965603	8.947049240272758E-5
130-131	0.12613882932396636	3.058642414697896E-4	3.7452764261606895E-5	5.617914639241034E-5	0.25730049047723935	9.571261977966206E-5
132-133	0.1261492328695946	3.162677870980138E-4	4.1614182512896545E-5	5.825985551805517E-5	0.2579725595248227	9.987403803095172E-5
134-135	0.1261637978334741	3.183484962236586E-4	4.57756007641862E-5	5.825985551805517E-5	0.25864670928153155	1.0195474715659654E-4
136-137	0.12616587854259975	3.2042920534930343E-4	4.57756007641862E-5	5.825985551805517E-5	0.25938328031200986	1.0403545628224136E-4
138-139	0.12616587854259975	3.2042920534930343E-4	4.57756007641862E-5	5.825985551805517E-5	0.26006159148697006	1.1027758365917586E-4
>>END_MODULE
