##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.4d.S.2.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24118417
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.63121136847414	37.0	35.0	39.0	34.0	39.0
2	35.91011267447611	36.0	35.0	38.0	34.0	39.0
3	36.050650339116366	36.0	35.0	38.0	34.0	39.0
4	36.163631676158516	37.0	35.0	38.0	34.0	39.0
5	36.01015021010707	37.0	35.0	38.0	34.0	39.0
6	35.92181845931265	37.0	35.0	38.0	34.0	39.0
7	35.93438412645407	36.0	35.0	38.0	34.0	39.0
8	36.120265065489164	37.0	35.0	39.0	34.0	39.0
9	36.11753756475809	37.0	35.0	39.0	34.0	39.0
10-14	36.031044135276375	36.8	35.0	38.4	34.0	39.0
15-19	36.04406859703935	37.0	35.0	38.0	34.0	39.0
20-24	35.971207480159244	37.0	35.0	38.0	34.0	39.0
25-29	35.974259388582595	37.0	35.0	38.0	34.0	39.0
30-34	35.9588024869128	37.0	35.0	38.0	34.0	39.0
35-39	35.8829453939701	37.0	35.0	38.0	34.0	39.0
40-44	35.869631327794025	36.6	35.0	38.0	33.8	39.0
45-49	35.869546803175346	36.2	35.0	38.0	33.8	39.0
50-54	35.81761423231052	36.2	35.0	38.0	33.2	39.0
55-59	35.846909737069396	36.0	35.0	38.0	33.4	39.0
60-64	35.76509855518295	36.0	35.0	38.0	33.0	39.0
65-69	35.75712740185229	36.0	35.0	38.0	33.0	39.0
70-74	35.72276622466558	36.0	35.0	38.0	33.0	39.0
75-79	35.66720351505656	36.0	35.0	38.0	33.0	39.0
80-84	35.64936018810853	36.0	35.0	38.0	33.0	39.0
85-89	35.5447954399329	36.0	35.0	38.0	33.0	39.0
90-94	35.502677004050476	36.0	35.0	38.0	32.8	39.0
95-99	35.4159981146358	36.0	34.8	38.0	32.2	39.0
100-104	35.358292669042086	36.0	34.2	38.0	32.0	39.0
105-109	35.215485037844736	36.0	34.0	38.0	31.8	39.0
110-114	35.11007588101657	36.0	34.0	38.0	31.4	39.0
115-119	35.053605632575305	36.0	34.0	38.0	31.4	39.0
120-124	35.01921840890304	36.0	34.0	38.0	31.4	39.0
125-129	34.97041065340234	36.0	34.0	38.0	31.0	39.0
130-134	34.87395382541068	36.0	34.0	38.0	30.8	39.0
135-139	34.715131735221256	36.0	34.0	38.0	30.4	39.0
140-144	34.62315572369447	36.0	34.0	38.0	29.8	39.0
145-149	34.65173473864392	36.0	34.0	38.0	30.2	39.0
150	34.36538716450586	36.0	34.0	38.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	141.0
22	1367.0
23	6913.0
24	22045.0
25	48625.0
26	89325.0
27	150984.0
28	232327.0
29	326827.0
30	442220.0
31	598152.0
32	830252.0
33	1215424.0
34	1936169.0
35	3566051.0
36	1.1188652E7
37	3462917.0
38	18.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	31.446512430728767	19.385608931133415	16.61891408544765	32.548964552690165
2	24.077251836221258	24.913985855705207	32.899422876717	18.109339431356545
3	20.309566751416565	28.571514457188464	30.80370075697754	20.31521803441743
4	23.460996631744116	33.392921268423216	22.373441839072605	20.772640260760067
5	25.979818658911153	34.31925072031054	22.32221542566413	17.37871519511417
6	19.632494951886766	38.72784851509948	22.293979741705268	19.345676791308485
7	20.652109962274885	20.592508206488013	37.877390543500425	20.87799128773667
8	21.765694655665005	23.157564611309276	28.73777329581788	26.338967437207838
9	23.81278588889146	22.624507238596962	28.663261772113817	24.899445100397756
10-14	23.17883963943405	28.596974668776976	25.787284463984516	22.436901227804462
15-19	23.254622390847622	27.65562764753591	27.054983749555372	22.0347662120611
20-24	23.10138099030297	27.79565590892636	26.91509231306516	22.18787078770551
25-29	23.299377401095605	27.41192840309544	26.862626183136314	22.42606801267264
30-34	23.148108766839883	27.227615311568748	27.02437477550869	22.59990114608268
35-39	23.491918229956802	27.011344898796636	26.95434198687252	22.542394884374044
40-44	23.58381895461879	26.72817954843388	27.04303271645067	22.64496878049666
45-49	23.554708420540205	26.548463773555287	27.154791294967655	22.74203651093685
50-54	23.68609214302926	26.52260648313152	26.966393412589508	22.824907961249714
55-59	23.76844798727877	26.4378155498348	27.03838730377703	22.755349159109407
60-64	23.542161991809	26.39839090600349	27.084330617552553	22.97511648463496
65-69	23.652305207261325	26.339720388780073	27.061908747991215	22.946065655967388
70-74	23.868635287917456	26.285795665139904	26.84547918200367	23.000089864938975
75-79	23.71270445434386	26.403804853456297	27.055194220794693	22.828296471405146
80-84	24.024371085382594	26.36877619289856	26.744846479766892	22.86200624195195
85-89	23.83142641575523	26.465532957656386	26.934445987893817	22.76859463869457
90-94	23.826460915739204	26.25080825163608	26.945057795459793	22.97767303716492
95-99	23.794348719608667	26.476822505762232	26.978319904538683	22.750508870090417
100-104	24.025232667633205	26.243429657924892	26.91991850045548	22.81141917398642
105-109	23.86617330648193	26.451376970553248	26.8714567792737	22.810992943691122
110-114	23.95608633850223	26.539092511751495	26.762523427636232	22.742297722110038
115-119	24.050112609381557	26.586337955648897	26.877788263448537	22.48576117152101
120-124	23.959419890617202	26.656809192742625	27.068603217201193	22.31516769943898
125-129	24.03833468838357	26.71203586868906	26.894628283439996	22.355001159487376
130-134	24.202920919823914	26.735785411979084	26.83763952958072	22.223654138616283
135-139	24.004177388590637	26.835471830510272	27.003851869714335	22.15649891118476
140-144	24.006301076890743	26.940199267638505	26.872882245961666	22.18061740950909
145-149	24.151935213259932	27.607510290642296	26.27957486828074	21.96097962781703
150	23.991317506451605	27.62303181008936	26.152997520525496	22.232653162933538
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	1.5
3	1.0
4	0.5
5	0.5
6	0.5
7	1.0
8	1.0
9	3.5
10	4.5
11	6.5
12	11.5
13	14.5
14	23.0
15	40.0
16	64.5
17	118.0
18	223.0
19	394.0
20	648.5
21	1053.0
22	1612.0
23	2482.0
24	4029.0
25	6262.0
26	9506.0
27	14660.0
28	21413.5
29	30013.0
30	42468.5
31	60159.0
32	86058.5
33	116308.5
34	153564.5
35	213638.5
36	300580.5
37	399233.0
38	514816.0
39	667004.5
40	852248.5
41	1045674.0
42	1235526.5
43	1414928.0
44	1549080.5
45	1625989.5
46	1641975.5
47	1592813.5
48	1524851.0
49	1455411.0
50	1334103.0
51	1168556.5
52	997223.0
53	825292.5
54	672221.0
55	559030.0
56	460012.0
57	378806.5
58	320336.5
59	262773.0
60	200246.0
61	136315.0
62	84937.0
63	53314.5
64	33535.0
65	17491.5
66	10240.0
67	5855.0
68	3536.5
69	2366.5
70	1681.0
71	1453.5
72	1276.5
73	1062.5
74	906.5
75	556.0
76	173.0
77	78.5
78	38.5
79	22.5
80	15.5
81	10.5
82	6.5
83	5.5
84	5.0
85	3.0
86	3.0
87	2.5
88	2.0
89	2.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.08126569832506006
55-59	0.0
60-64	0.0
65-69	0.0
70-74	3.31696727857388E-6
75-79	2.48772545893041E-6
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.08303530036817922
100-104	0.0
105-109	0.0
110-114	0.0
115-119	8.2924181964347E-7
120-124	0.0
125-129	0.0
130-134	3.31696727857388E-6
135-139	0.0
140-144	0.0
145-149	8.2924181964347E-7
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4118417E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.01930296877325
#Duplication Level	Percentage of total
1	22.123914578952885
2	9.612814661133564
3	6.187546113658758
4	4.311961630141418
5	3.2910244243051214
6	2.58111107055959
7	2.148134105986625
8	1.7834764102906038
9	1.483303420185267
>10	18.64314334488217
>50	6.396450388006672
>100	12.928992596291202
>500	3.6081209313524174
>1k	4.170263496246253
>5k	0.5256959081108719
>10k+	0.20404691989667165
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.0731045491086749E-4	0.0	0.0	0.0	0.001766285075840591	0.0
2	9.121660016078169E-4	2.073104549108675E-5	0.0	8.292418196434699E-6	0.009362140143774777	0.0
3	0.0014180035115903336	2.48772545893041E-5	0.0	8.292418196434699E-6	0.012894710295455959	0.0
4	0.0040093841979761775	2.48772545893041E-5	0.0	8.292418196434699E-6	0.0182101503593706	0.0
5	0.004158647725512002	2.902346368752145E-5	0.0	8.292418196434699E-6	0.021751012929248217	4.1462090982173495E-6
6	0.005108129609003775	2.902346368752145E-5	0.0	8.292418196434699E-6	0.028484456504753192	4.1462090982173495E-6
7	0.006762467039192498	3.3169672785738796E-5	4.1462090982173495E-6	8.292418196434699E-6	0.037291004629366846	4.1462090982173495E-6
8	0.012011567757535662	3.3169672785738796E-5	4.1462090982173495E-6	8.292418196434699E-6	0.05086154700783223	8.292418196434699E-6
9	0.012554721149402136	4.14620909821735E-5	4.1462090982173495E-6	8.292418196434699E-6	0.06031075754266957	8.292418196434699E-6
10-11	0.013064704868482869	4.560830008039085E-5	4.1462090982173495E-6	8.292418196434699E-6	0.1033338962503219	8.292418196434699E-6
12-13	0.013512495451090343	4.560830008039085E-5	4.1462090982173495E-6	8.292418196434699E-6	0.12509320159776655	8.292418196434699E-6
14-15	0.015243537749596088	4.560830008039085E-5	8.292418196434699E-6	8.292418196434699E-6	0.1356453037527297	8.292418196434699E-6
16-17	0.0172544491622315	4.560830008039085E-5	1.243862729465205E-5	8.292418196434699E-6	0.14233106592360517	8.292418196434699E-6
18-19	0.022144902793578863	5.182761372771687E-5	1.243862729465205E-5	8.292418196434699E-6	0.14924486959488262	8.292418196434699E-6
20-21	0.026884019792841295	5.3900718276825546E-5	1.243862729465205E-5	8.292418196434699E-6	0.1544504351176945	8.292418196434699E-6
22-23	0.02911675339223134	5.3900718276825546E-5	1.6584836392869398E-5	8.292418196434699E-6	0.15960002681768043	8.292418196434699E-6
24-25	0.03365477925023023	6.012003192415157E-5	1.6584836392869398E-5	8.292418196434699E-6	0.16497973312261746	8.292418196434699E-6
26-27	0.03746514541149197	7.048555466969495E-5	1.6584836392869398E-5	8.292418196434699E-6	0.16946593136688864	1.243862729465205E-5
28-29	0.04573268635333737	7.46317637679123E-5	1.6584836392869398E-5	8.292418196434699E-6	0.17301508635496268	1.243862729465205E-5
30-31	0.06553083479732522	7.877797286612965E-5	2.073104549108675E-5	8.292418196434699E-6	0.17688764565269768	1.4511731843760724E-5
32-33	0.08379073966587441	9.12166001607817E-5	2.6950359138412773E-5	8.292418196434699E-6	0.1808327636096515	1.6584836392869398E-5
34-35	0.09776139122231778	9.12166001607817E-5	2.902346368752145E-5	1.0365522745543375E-5	0.18449801245247563	1.6584836392869398E-5
36-37	0.1001807042311276	9.95090183572164E-5	3.31696727857388E-5	1.243862729465205E-5	0.18672038052912016	1.8657940941978076E-5
38-39	0.10226417430298182	1.0158212290632507E-4	3.9388986433064826E-5	1.243862729465205E-5	0.18851776217319735	2.073104549108675E-5
40-41	0.10349352530060327	1.0780143655365109E-4	4.14620909821735E-5	1.243862729465205E-5	0.19016795339428785	2.073104549108675E-5
42-43	0.1037858330420276	1.1402075020097713E-4	4.14620909821735E-5	1.243862729465205E-5	0.19174765906070868	2.073104549108675E-5
44-45	0.10455702793429603	1.160938547500858E-4	4.14620909821735E-5	1.243862729465205E-5	0.19303298388115603	2.073104549108675E-5
46-47	0.10594600798219883	1.2024006384830315E-4	4.14620909821735E-5	1.243862729465205E-5	0.19458159297934022	2.073104549108675E-5
48-49	0.1072292596980971	1.2853248204473783E-4	4.14620909821735E-5	1.243862729465205E-5	0.19592289162261356	2.073104549108675E-5
50-51	0.1082077650452764	1.2853248204473783E-4	4.14620909821735E-5	1.243862729465205E-5	0.1973678454933423	2.073104549108675E-5
52-53	0.10909712689684402	1.3060558659384652E-4	4.14620909821735E-5	1.243862729465205E-5	0.19862207374555302	2.073104549108675E-5
54-55	0.10957601404768812	1.3267869114295518E-4	4.14620909821735E-5	1.243862729465205E-5	0.19977264677030834	2.2804150040195424E-5
56-57	0.11052134972208168	1.3267869114295518E-4	4.14620909821735E-5	1.243862729465205E-5	0.20106211779985395	2.48772545893041E-5
58-59	0.11074317190883631	1.3267869114295518E-4	4.14620909821735E-5	1.6584836392869398E-5	0.2021256204335467	2.48772545893041E-5
60-61	0.11104169896390795	1.3682490024117253E-4	4.14620909821735E-5	1.6584836392869398E-5	0.20305437127154738	2.6950359138412773E-5
62-63	0.11128010598705546	1.430442138884986E-4	4.14620909821735E-5	1.6584836392869398E-5	0.20402665730507935	2.902346368752145E-5
64-65	0.11131949497348852	1.492635275358246E-4	4.14620909821735E-5	1.6584836392869398E-5	0.20512332961155783	3.3169672785738796E-5
66-67	0.11141900399184573	1.59629050281368E-4	4.14620909821735E-5	1.6584836392869398E-5	0.20599403352218348	3.3169672785738796E-5
68-69	0.11147290471012256	1.6999457302691135E-4	4.14620909821735E-5	1.6584836392869398E-5	0.20677144772809924	3.3169672785738796E-5
70-71	0.11159107166942175	1.6999457302691135E-4	4.14620909821735E-5	1.6584836392869398E-5	0.20755922745676053	3.3169672785738796E-5
72-73	0.11165533791044413	1.6999457302691135E-4	4.14620909821735E-5	1.6584836392869398E-5	0.20809201532588145	3.3169672785738796E-5
74-75	0.1116739958513861	1.7206767757602E-4	4.14620909821735E-5	1.6584836392869398E-5	0.20884662538175702	3.3169672785738796E-5
76-77	0.11172789656966292	1.7621388667423738E-4	4.560830008039085E-5	1.6584836392869398E-5	0.209800253474347	3.3169672785738796E-5
78-79	0.11177557797429243	1.7828699122334605E-4	4.560830008039085E-5	1.6584836392869398E-5	0.21083265953980312	3.3169672785738796E-5
80-81	0.11181289385617638	1.803600957724547E-4	4.97545091786082E-5	1.8657940941978076E-5	0.21167226688219215	3.9388986433064826E-5
82-83	0.11184399042441301	1.8450630487067208E-4	4.97545091786082E-5	2.48772545893041E-5	0.21244138866991147	4.14620909821735E-5
84-85	0.11187301388810053	1.8657940941978074E-4	4.97545091786082E-5	2.902346368752145E-5	0.2133369698351264	4.3535195531282174E-5
86-87	0.11190825666543538	1.886525139688894E-4	4.97545091786082E-5	2.902346368752145E-5	0.21425328204583244	4.560830008039085E-5
88-89	0.11194142633822113	1.907256185179981E-4	4.97545091786082E-5	2.902346368752145E-5	0.21523386049756085	4.768140462949952E-5
90-91	0.11198288842920329	1.9487182761621544E-4	4.97545091786082E-5	2.902346368752145E-5	0.21588274222143186	4.97545091786082E-5
92-93	0.11201191189289081	1.9487182761621544E-4	4.97545091786082E-5	2.902346368752145E-5	0.21647357701792783	5.182761372771687E-5
94-95	0.1120575201929712	1.9487182761621544E-4	4.97545091786082E-5	2.902346368752145E-5	0.21719916361011588	6.219313647326024E-5
96-97	0.11206373950661853	1.9487182761621544E-4	4.97545091786082E-5	2.902346368752145E-5	0.21788121500677263	6.633934557147759E-5
98-99	0.11208447055210961	1.969449321653241E-4	4.97545091786082E-5	2.902346368752145E-5	0.21856119329888027	7.048555466969495E-5
100-101	0.11211971332944447	1.990180367144328E-4	4.97545091786082E-5	2.902346368752145E-5	0.21911056600439405	7.46317637679123E-5
102-103	0.11214251747948467	2.0109114126354148E-4	5.182761372771687E-5	3.109656823663012E-5	0.21975737462371597	7.877797286612965E-5
104-105	0.11216324852497575	2.052373503617588E-4	5.3900718276825546E-5	3.3169672785738796E-5	0.22039174461574323	8.085107741523833E-5
106-107	0.11217361404772129	2.1560287310730218E-4	5.3900718276825546E-5	3.731588188395615E-5	0.22094733663490435	8.707039106256435E-5
108-109	0.11221507613870346	2.2804150040195423E-4	5.3900718276825546E-5	3.731588188395615E-5	0.22155061005869497	9.328970470989037E-5
110-111	0.11224409960239098	2.3218770950017158E-4	5.3900718276825546E-5	3.9388986433064826E-5	0.22218498005072224	1.0365522745543374E-4
112-113	0.11225446512513654	2.404801276966063E-4	5.3900718276825546E-5	4.14620909821735E-5	0.2227923996836111	1.0365522745543374E-4
114-115	0.11226483064788206	2.404801276966063E-4	5.3900718276825546E-5	4.14620909821735E-5	0.2232816523572007	1.0780143655365109E-4
116-117	0.11227726927517673	2.404801276966063E-4	5.3900718276825546E-5	4.14620909821735E-5	0.2238268788536163	1.1402075020097713E-4
118-119	0.11228763479792227	2.4462633679482365E-4	5.3900718276825546E-5	4.14620909821735E-5	0.22435137430454077	1.1816695929919448E-4
120-121	0.11230007342521692	2.4462633679482365E-4	6.012003192415157E-5	4.14620909821735E-5	0.22488830838275994	1.2024006384830315E-4
122-123	0.11231665826160979	2.48772545893041E-4	6.219313647326024E-5	4.14620909821735E-5	0.22555999425667117	1.2231316839741183E-4
124-125	0.11233531620255177	2.5291875499125835E-4	6.219313647326024E-5	4.560830008039085E-5	0.2262358263396806	1.2853248204473783E-4
126-127	0.11234775482984642	2.5706496408947567E-4	6.219313647326024E-5	4.560830008039085E-5	0.22684739218166766	1.409711093393899E-4
128-129	0.11235190103894463	2.653573822859104E-4	6.219313647326024E-5	4.560830008039085E-5	0.22750456632373509	1.59629050281368E-4
130-131	0.11236019345714107	2.7572290503145375E-4	6.219313647326024E-5	4.560830008039085E-5	0.2281969832431374	1.65848363928694E-4
132-133	0.1123892169208286	2.819422186787798E-4	6.219313647326024E-5	4.560830008039085E-5	0.2287442828441021	1.741407821251287E-4
134-135	0.112416167279967	2.8608842777699714E-4	6.633934557147759E-5	4.560830008039085E-5	0.22930194796781234	1.7828699122334605E-4
136-137	0.11245762937094918	2.881615323261058E-4	6.633934557147759E-5	4.560830008039085E-5	0.2299218062279958	1.8450630487067208E-4
138-139	0.11248250662553849	2.902346368752145E-4	6.633934557147759E-5	4.560830008039085E-5	0.23046703272441138	1.886525139688894E-4
>>END_MODULE
