##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.4d.S.3.R1.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24075394
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.26538228200959	37.0	35.0	39.0	33.0	39.0
2	35.69423507669283	36.0	35.0	39.0	33.0	39.0
3	35.896828936631316	37.0	35.0	39.0	33.0	39.0
4	35.97093139991811	37.0	35.0	39.0	33.0	39.0
5	35.794877624848006	36.0	35.0	38.0	33.0	39.0
6	35.65426630193466	36.0	35.0	38.0	33.0	39.0
7	35.49366257515869	35.0	35.0	37.0	33.0	39.0
8	35.74040441456535	36.0	35.0	38.0	33.0	39.0
9	35.73243935280976	36.0	35.0	38.0	33.0	39.0
10-14	35.75405558056495	36.2	35.0	38.2	33.0	39.0
15-19	35.75353176774594	36.0	35.0	38.0	33.0	39.0
20-24	35.74967485890366	36.0	35.0	38.0	33.0	39.0
25-29	35.74421864913197	36.0	35.0	38.0	33.0	39.0
30-34	35.731464157969754	36.0	35.0	38.0	33.0	39.0
35-39	35.726238033736855	36.0	35.0	38.0	33.0	39.0
40-44	35.71512292592179	36.0	35.0	38.0	33.0	39.0
45-49	35.70448804285404	36.0	35.0	38.0	33.0	39.0
50-54	35.69002223598085	36.0	35.0	38.0	33.0	39.0
55-59	35.68307694569817	36.0	35.0	38.0	33.0	39.0
60-64	35.6721968413061	36.0	35.0	38.0	33.0	39.0
65-69	35.654953518102346	36.0	35.0	38.0	33.0	39.0
70-74	35.65265511334934	36.0	35.0	38.0	33.0	39.0
75-79	35.64360335702086	36.0	35.0	38.0	33.0	39.0
80-84	35.62095721465659	36.0	35.0	38.0	33.0	39.0
85-89	35.60559250660654	36.0	35.0	38.0	33.0	39.0
90-94	35.59841028562191	36.0	35.0	38.0	33.0	39.0
95-99	35.57175188908642	36.0	35.0	38.0	33.0	39.0
100-104	35.551381746857395	36.0	35.0	38.0	33.0	39.0
105-109	35.52559984688101	36.0	35.0	38.0	33.0	39.0
110-114	35.508466885318676	36.0	35.0	38.0	33.0	39.0
115-119	35.47433337954926	36.0	34.2	38.0	33.0	39.0
120-124	35.45415069011955	36.0	34.0	38.0	32.4	39.0
125-129	35.42748859686367	36.0	34.0	38.0	32.0	39.0
130-134	35.392072486954945	36.0	34.0	38.0	32.0	39.0
135-139	35.35812262096313	36.0	34.0	38.0	32.0	39.0
140-144	35.321477521821656	36.0	34.0	38.0	32.0	39.0
145-149	35.28661850352272	36.0	34.0	38.0	32.0	39.0
150	35.26551391017734	36.0	34.0	38.0	32.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	7.0
22	141.0
23	1716.0
24	11666.0
25	45271.0
26	112170.0
27	199699.0
28	290499.0
29	375421.0
30	468610.0
31	593291.0
32	771490.0
33	1065345.0
34	1598990.0
35	2909047.0
36	1.0750596E7
37	4881384.0
38	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.77023984549829	10.749243250311148	15.107920422447341	38.37259648174322
2	23.537104523451845	11.819054117295808	35.302111665818565	29.341729693433777
3	21.73262357717684	16.544871253027267	27.965491910985428	33.75701325881046
4	26.787112674029213	23.861136920600615	21.786466565668018	27.565283839702154
5	27.206125309814144	29.901910757493116	23.72296510605196	19.168998826640774
6	20.122936362009277	36.626059074532684	24.625847073751643	18.625157489706396
7	16.44759222472021	25.34662176436025	39.682205612721845	18.523580398197694
8	18.86741426004907	24.540897307279007	30.934318937427673	25.657369495244254
9	19.800814059367003	22.860336158984563	32.39933269627903	24.939517085369403
10-14	20.622145600656843	29.172304168452516	26.624027091177343	23.581523139713298
15-19	20.974490187418215	27.845216824057147	27.277757608262043	23.902535380262595
20-24	21.001994604009	27.938241177019062	27.100933489031142	23.9588307299408
25-29	20.955013860398942	28.13422068507805	26.820659063062052	24.090106391460957
30-34	20.927000125555946	28.031451257419445	26.93490940242232	24.10663921460229
35-39	21.140766706131647	27.76411908743847	26.890864150189785	24.20425005624009
40-44	21.244323377199283	27.812418955009534	26.802740271961518	24.140517395829665
45-49	21.46963120197909	27.697193960337557	26.738023284343605	24.09515155333975
50-54	21.482083628334387	27.837753537148707	26.661584785738707	24.018578048778195
55-59	21.400026852387317	27.77550583446286	26.78708228375949	24.037385029390336
60-64	21.622351787294228	27.657380212963133	26.539896350803794	24.180371648938845
65-69	21.533277018165442	27.703369178074766	26.70672196169846	24.056631842061332
70-74	21.814303788175778	27.510776626821205	26.640451359235108	24.034468225767906
75-79	21.66112082589273	27.476320209414617	26.615259873634344	24.247299091058306
80-84	21.92870765041329	27.391479013367103	26.60970279393145	24.070110542288155
85-89	21.794569208725502	27.402579813889922	26.67877353766015	24.124077439724424
90-94	21.80158366373437	27.18445799444678	26.642136225525586	24.371822116293263
95-99	22.009009871166267	27.221080529929093	26.759948714395776	24.009960884508867
100-104	21.998320358038956	27.043822256680677	26.806752577707115	24.15110480757325
105-109	22.087355340171282	26.96186331050525	26.757191961965322	24.193589387358145
110-114	22.01041333476794	26.9853616653467	26.75973763982843	24.24448736005693
115-119	22.142749024062773	27.164502749785342	26.638930769172553	24.05381745697933
120-124	22.05521327194143	27.02144255692098	26.716429419088477	24.206914752049112
125-129	22.37902677963256	26.97428062205869	26.63915941571605	24.0075331825927
130-134	22.151184820843437	27.132702564539695	26.539705443424825	24.17640717119204
135-139	22.29381097413294	26.961981856913695	26.557353930509	24.186853238444368
140-144	22.268740322055415	26.80915176346273	26.78919276804168	24.13291514644018
145-149	22.17597769855541	27.2530392407253	26.703362615393523	23.867620445325766
150	21.948585189714812	26.87827603145173	26.768648888347624	24.404489890485838
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.0
9	1.5
10	3.5
11	9.0
12	11.5
13	14.5
14	29.0
15	74.0
16	136.0
17	273.5
18	651.0
19	1170.5
20	1940.5
21	3133.5
22	5086.5
23	8188.5
24	12892.0
25	19352.5
26	27308.0
27	38437.0
28	57087.0
29	83702.5
30	114433.5
31	145113.0
32	177768.5
33	214996.5
34	251848.5
35	298748.5
36	369021.5
37	447838.0
38	529260.0
39	639423.5
40	785733.5
41	948861.0
42	1110450.0
43	1263752.0
44	1399773.5
45	1494525.0
46	1547252.5
47	1551392.0
48	1493850.5
49	1425209.0
50	1326168.0
51	1176281.0
52	1002135.5
53	846008.0
54	699985.5
55	565692.0
56	475297.5
57	392245.5
58	320054.0
59	257567.0
60	197840.0
61	137664.5
62	80334.5
63	45891.5
64	30247.0
65	19769.5
66	13740.0
67	7151.5
68	3405.5
69	2159.0
70	1627.0
71	1575.5
72	1582.5
73	1213.5
74	630.5
75	187.5
76	59.5
77	39.0
78	24.0
79	17.5
80	18.0
81	12.0
82	5.5
83	3.5
84	1.5
85	2.5
86	2.5
87	0.5
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.5752434207307264E-4
2	1.702983552418706E-4
3	0.0019065108550248443
4	0.002014504933958713
5	3.0321414469894033E-4
6	4.568980262586772E-5
7	6.645789472853486E-5
8	2.9075328943734005E-5
9	0.0
10-14	5.648921051925464E-5
15-19	3.0736776311947375E-5
20-24	1.6116039471669705E-4
25-29	1.6946763155776393E-4
30-34	2.9241473680555343E-4
35-39	1.7445197366240404E-4
40-44	1.3208506577296305E-4
45-49	3.115213815400072E-4
50-54	2.3343335523397872E-4
55-59	2.7912315785984644E-4
60-64	6.105819078184141E-4
65-69	7.268832235933501E-4
70-74	3.9957809205531594E-4
75-79	2.2595684207701855E-4
80-84	2.6832374996645954E-4
85-89	1.2211638156368283E-4
90-94	2.3177190786576537E-4
95-99	9.137960525173544E-5
100-104	1.0384046051333573E-4
105-109	6.811934209674824E-5
110-114	1.345772368252831E-4
115-119	9.885611840869561E-5
120-124	1.5617605261205694E-4
125-129	1.8691282892400432E-4
130-134	1.3540796050938978E-4
135-139	1.7195980261008398E-4
140-144	1.412230262981366E-4
145-149	1.6116039471669705E-4
150	6.313499999210813E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4075394E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.4046721512925
#Duplication Level	Percentage of total
1	32.038273950051796
2	9.740571429213475
3	5.628846523128343
4	3.922144068384492
5	2.971182441733284
6	2.3127424910088425
7	1.860234625612668
8	1.6233179798852022
9	1.4361900486648451
>10	16.624086746011304
>50	5.188153461863458
>100	10.482149350576913
>500	3.2513128410929024
>1k	2.8415735347131306
>5k	0.07922050805912603
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.153618420533429E-6	2.0768092102667146E-5	0.0	0.0	1.993736841856046E-4	4.153618420533429E-6
2	4.153618420533429E-6	9.553322367226888E-5	0.0	1.2460855261600288E-5	0.002110038157630982	1.2460855261600288E-5
3	1.2460855261600288E-5	1.0384046051333573E-4	0.0	1.2460855261600288E-5	0.003850404275834489	3.322894736426743E-5
4	4.984342104640115E-5	1.2460855261600288E-4	0.0	2.0768092102667146E-5	0.005586616775617463	3.738256578480086E-5
5	4.984342104640115E-5	1.2460855261600288E-4	0.0	2.4921710523200576E-5	0.006986386183337228	4.153618420533429E-5
6	8.722598683120202E-5	1.2460855261600288E-4	0.0	2.4921710523200576E-5	0.008556453946298864	4.153618420533429E-5
7	1.1630131577493602E-4	1.4537664471867004E-4	0.0	2.4921710523200576E-5	0.010230362169773837	4.153618420533429E-5
8	2.0352730260613804E-4	1.4953026313920345E-4	0.0	2.4921710523200576E-5	0.01323758190624004	6.645789472853486E-5
9	2.450634868114723E-4	1.4953026313920345E-4	0.0	2.4921710523200576E-5	0.01608281052430544	6.645789472853486E-5
10-11	3.0321414469894033E-4	1.5783749998027032E-4	0.0	2.4921710523200576E-5	0.02813038075306265	9.761003288253558E-5
12-13	3.6344161179667505E-4	1.6199111840080373E-4	0.0	2.4921710523200576E-5	0.03725172680455406	1.2045493419546944E-4
14-15	4.0497779600200937E-4	1.6822154603160388E-4	0.0	2.9075328943734002E-5	0.044553787987851826	1.3499259866733645E-4
16-17	4.3197631573547665E-4	1.7860559208293745E-4	0.0	3.322894736426743E-5	0.049639893743795016	1.3706940787760316E-4
18-19	4.735124999408109E-4	1.9314325655480448E-4	0.0	3.322894736426743E-5	0.0533469981841211	1.3706940787760316E-4
20-21	6.91577467018816E-4	2.2429539470880517E-4	0.0	3.322894736426743E-5	0.05649959456530597	1.3706940787760316E-4
22-23	9.449481906713551E-4	2.284490131293386E-4	0.0	3.5305756574534143E-5	0.05957950262413151	1.4329983550840332E-4
24-25	0.0010342509867128239	2.3467944076013876E-4	0.0	3.738256578480086E-5	0.06275702071583958	1.4953026313920345E-4
26-27	0.0010944784538105587	2.3675624997040548E-4	0.0	4.153618420533429E-5	0.06562924785363845	1.5160707234947017E-4
28-29	0.0011443218748569597	2.3883305918067217E-4	0.0	4.568980262586772E-5	0.06891476002428039	1.6406792761107045E-4
30-31	0.001237778289318962	2.450634868114723E-4	0.0	5.60738486772013E-5	0.07240172268831821	1.7652878287267073E-4
32-33	0.001273084045893496	2.450634868114723E-4	0.0	6.645789472853488E-5	0.07571631018790388	2.0352730260613804E-4
34-35	0.0013312347037809642	2.4921710523200576E-4	0.0	1.0176365130306901E-4	0.07920119604273143	2.1806496707800504E-4
36-37	0.0013665404603554982	2.512939144422725E-4	0.0	1.1214769735440259E-4	0.0823849445620703	2.242953947088052E-4
38-39	0.0013706940787760317	2.533707236525392E-4	0.0	1.1630131577493602E-4	0.08529455426565397	2.3052582233960533E-4
40-41	0.0014059998353505659	2.533707236525392E-4	0.0	1.1630131577493602E-4	0.08820208716002736	2.3260263154987204E-4
42-43	0.0014163838814018994	2.554475328628059E-4	0.0	1.1630131577493602E-4	0.09083548123864557	2.3260263154987204E-4
44-45	0.001426767927453233	2.61677960493606E-4	0.0	1.1837812498520273E-4	0.09370770837644443	2.3675624997040548E-4
46-47	0.0014454592103456333	2.61677960493606E-4	2.0768092102667145E-6	1.2045493419546944E-4	0.09810431347457907	2.409098683909389E-4
48-49	0.0014745345392893673	2.679083881244062E-4	4.153618420533429E-6	1.2876217103653632E-4	0.10113645492156847	2.4714029602173904E-4
50-51	0.0014849185853407008	2.8659967101680664E-4	4.153618420533429E-6	1.2876217103653632E-4	0.1042412846909172	2.533707236525392E-4
52-53	0.0015243779603357687	2.9490690785787346E-4	4.153618420533429E-6	1.2876217103653632E-4	0.10695982794715633	2.533707236525392E-4
54-55	0.0015534532892795025	2.9490690785787346E-4	4.153618420533429E-6	1.3083898024680304E-4	0.10916539932845959	2.533707236525392E-4
56-57	0.0016261416116388375	2.990605262784069E-4	4.153618420533429E-6	1.3291578945706973E-4	0.11149765607158911	2.554475328628059E-4
58-59	0.0016988299339981724	3.0321414469894033E-4	4.153618420533429E-6	1.3291578945706973E-4	0.1137572244923593	2.5752434207307264E-4
60-61	0.0017819023024088412	3.115213815400072E-4	4.153618420533429E-6	1.3706940787760316E-4	0.11601471610391921	2.5752434207307264E-4
62-63	0.0018213616774039086	3.1567499996054064E-4	4.153618420533429E-6	1.3706940787760316E-4	0.11864187975490662	2.61677960493606E-4
64-65	0.001840052960296309	3.198286183810741E-4	4.153618420533429E-6	1.4329983550840332E-4	0.12194192959002043	2.61677960493606E-4
66-67	0.0018525138155579095	3.3228947364267433E-4	4.153618420533429E-6	1.4537664471867004E-4	0.12510698682646687	2.61677960493606E-4
68-69	0.0018670514800297766	3.426735196940079E-4	4.153618420533429E-6	1.4537664471867004E-4	0.1273603248196063	2.6583157891413946E-4
70-71	0.0019044340458145774	3.6551842100694177E-4	4.153618420533429E-6	1.4537664471867004E-4	0.12960950919432512	2.699851973346729E-4
72-73	0.0019210485194967111	3.7382565784800864E-4	4.153618420533429E-6	1.4537664471867004E-4	0.13153886495066291	2.7621562496547305E-4
74-75	0.0019522006576507117	3.7382565784800864E-4	4.153618420533429E-6	1.5783749998027032E-4	0.13362190458856044	2.845228618065399E-4
76-77	0.0019812759865944457	3.7382565784800864E-4	4.153618420533429E-6	1.5783749998027032E-4	0.13522935491730687	2.907532894373401E-4
78-79	0.0020041208879073794	3.7590246705827536E-4	4.153618420533429E-6	1.6199111840080373E-4	0.13686380376578677	2.9490690785787346E-4
80-81	0.0020269657892203136	3.821328946890755E-4	4.153618420533429E-6	1.6199111840080373E-4	0.13839233534454307	2.9490690785787346E-4
82-83	0.0020643483550051144	3.821328946890755E-4	4.153618420533429E-6	1.6199111840080373E-4	0.1401680072193211	2.9490690785787346E-4
84-85	0.0021308062497336494	3.821328946890755E-4	4.153618420533429E-6	1.6199111840080373E-4	0.14195406314015047	2.9490690785787346E-4
86-87	0.0021661120063081836	3.821328946890755E-4	4.153618420533429E-6	1.6199111840080373E-4	0.14366535392941027	2.9490690785787346E-4
88-89	0.0021868800984108506	3.821328946890755E-4	4.153618420533429E-6	1.723751644521373E-4	0.14538079833709056	3.0321414469894033E-4
90-91	0.002199340953672451	3.821328946890755E-4	4.153618420533429E-6	1.7860559208293745E-4	0.14772551593548167	3.0736776311947377E-4
92-93	0.002228416282616185	3.821328946890755E-4	4.153618420533429E-6	1.7860559208293745E-4	0.1498895511325796	3.0736776311947377E-4
94-95	0.0022574916115599185	3.821328946890755E-4	4.153618420533429E-6	1.8068240129320417E-4	0.15178983155997364	3.094445723297405E-4
96-97	0.0022948741773447193	3.821328946890755E-4	4.153618420533429E-6	1.8275921050347089E-4	0.15335366889530447	3.115213815400072E-4
98-99	0.002373792927334855	3.842097038993422E-4	4.153618420533429E-6	1.9106644734453776E-4	0.15483858748064516	3.115213815400072E-4
100-101	0.0024838638154789905	3.862865131096089E-4	4.153618420533429E-6	1.9522006576507117E-4	0.15641073205281708	3.115213815400072E-4
102-103	0.002517092762843258	3.883633223198756E-4	4.153618420533429E-6	1.9522006576507117E-4	0.15771081461844405	3.115213815400072E-4
104-105	0.0025337072365253918	3.9044013153014234E-4	4.153618420533429E-6	2.0560411181640473E-4	0.15897351461828618	3.198286183810741E-4
106-107	0.0025731666115204593	3.987473683712092E-4	4.153618420533429E-6	2.1598815786773832E-4	0.16031928698653902	3.198286183810741E-4
108-109	0.0025897810852025934	3.987473683712092E-4	4.153618420533429E-6	2.3883305918067217E-4	0.1616255999797968	3.198286183810741E-4
110-111	0.002620933223356594	4.0497779600200937E-4	4.153618420533429E-6	2.450634868114723E-4	0.16274915376255109	3.198286183810741E-4
112-113	0.0027040055917672626	4.091314144225428E-4	4.153618420533429E-6	2.4714029602173904E-4	0.16395785672292632	3.198286183810741E-4
114-115	0.0027393113483417967	4.153618420533429E-4	4.153618420533429E-6	2.512939144422725E-4	0.16555076938720087	3.198286183810741E-4
116-117	0.002766309868075264	4.1951546047387634E-4	4.153618420533429E-6	2.6583157891413946E-4	0.16673870425547344	3.198286183810741E-4
118-119	0.0027891547693881975	4.3197631573547665E-4	4.153618420533429E-6	2.679083881244062E-4	0.16767534520930372	3.198286183810741E-4
120-121	0.0028743039470091334	4.3197631573547665E-4	4.153618420533429E-6	2.7829243417573977E-4	0.16876151642627324	3.198286183810741E-4
122-123	0.0030321414469894033	4.3197631573547665E-4	4.153618420533429E-6	2.9490690785787346E-4	0.16979161379456553	3.2190542759134074E-4
124-125	0.003135981907502739	4.3405312494574337E-4	4.153618420533429E-6	2.990605262784069E-4	0.1709276284325814	3.2398223680160746E-4
126-127	0.0031858253285491404	4.402835525765435E-4	4.153618420533429E-6	3.011373354886736E-4	0.17222355737978784	3.2398223680160746E-4
128-129	0.0032502064140674082	4.402835525765435E-4	4.153618420533429E-6	3.0321414469894033E-4	0.173237040274398	3.281358552221409E-4
130-131	0.00331874111800621	4.402835525765435E-4	4.153618420533429E-6	3.094445723297405E-4	0.17399299882693509	3.302126644324076E-4
132-133	0.0033623541114218107	4.465139802073436E-4	4.153618420533429E-6	3.2190542759134074E-4	0.17480087760972884	3.3228947364267433E-4
134-135	0.0034184279600990124	4.4859078941761034E-4	4.153618420533429E-6	3.2398223680160746E-4	0.17566898385962032	3.3436628285294105E-4
136-137	0.0035534205587663486	4.6105164467921065E-4	4.153618420533429E-6	3.302126644324076E-4	0.17667000589896886	3.489039473248081E-4
138-139	0.00361572483507435	4.7766611836134434E-4	4.153618420533429E-6	3.3436628285294105E-4	0.1774363484975573	3.489039473248081E-4
>>END_MODULE
