##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	sesquia21.lowPi.4d.S.3.R2.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24075394
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.63397836812141	37.0	35.0	39.0	34.0	39.0
2	35.93164344475525	36.0	35.0	38.0	34.0	39.0
3	36.06028437167009	36.0	35.0	38.0	34.0	39.0
4	36.1145752381041	37.0	35.0	38.0	34.0	39.0
5	35.957340095867174	37.0	35.0	38.0	34.0	39.0
6	35.877098086120625	36.0	35.0	38.0	34.0	39.0
7	35.973182827246774	36.0	35.0	38.0	34.0	39.0
8	36.09773102778713	37.0	35.0	39.0	34.0	39.0
9	36.11026606667372	37.0	35.0	39.0	34.0	39.0
10-14	36.024897453391624	36.8	35.0	38.4	34.0	39.0
15-19	36.02149073863547	37.0	35.0	38.0	34.0	39.0
20-24	35.94731378435593	36.8	35.0	38.0	34.0	39.0
25-29	35.94782580089863	36.8	35.0	38.0	34.0	39.0
30-34	35.929410933004874	36.8	35.0	38.0	34.0	39.0
35-39	35.849297319910946	36.2	35.0	38.0	33.6	39.0
40-44	35.84880023978008	36.0	35.0	38.0	33.6	39.0
45-49	35.868024647903994	36.2	35.0	38.0	33.8	39.0
50-54	35.81656387430253	36.0	35.0	38.0	33.0	39.0
55-59	35.831656354201314	36.0	35.0	38.0	33.4	39.0
60-64	35.731120105448746	36.0	35.0	38.0	33.0	39.0
65-69	35.72663888283614	36.0	35.0	38.0	33.0	39.0
70-74	35.701182850839324	36.0	35.0	38.0	33.0	39.0
75-79	35.65461885275896	36.0	35.0	38.0	33.0	39.0
80-84	35.633345057613596	36.0	35.0	38.0	33.0	39.0
85-89	35.52546605883168	36.0	35.0	38.0	32.8	39.0
90-94	35.46490071979715	36.0	35.0	38.0	32.4	39.0
95-99	35.37824432696719	36.0	34.6	38.0	32.2	39.0
100-104	35.328760426516794	36.0	34.0	38.0	32.0	39.0
105-109	35.21121342396307	36.0	34.0	38.0	31.8	39.0
110-114	35.04736248968553	36.0	34.0	38.0	31.4	39.0
115-119	35.034300381543076	36.0	34.0	38.0	31.4	39.0
120-124	34.99858431392649	36.0	34.0	38.0	31.2	39.0
125-129	34.919329428212066	36.0	34.0	38.0	30.8	39.0
130-134	34.84148064201982	36.0	34.0	38.0	30.8	39.0
135-139	34.64403740183858	35.8	34.0	38.0	30.0	39.0
140-144	34.60232159025103	36.0	34.0	38.0	29.8	39.0
145-149	34.57967543127227	36.0	34.0	38.0	30.0	39.0
150	34.37382960378551	35.0	34.0	38.0	29.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	145.0
22	1315.0
23	7180.0
24	22545.0
25	49538.0
26	90569.0
27	152525.0
28	233413.0
29	329754.0
30	448809.0
31	610427.0
32	849252.0
33	1246964.0
34	1979492.0
35	3617379.0
36	1.1083608E7
37	3352457.0
38	14.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.131989449476926	19.296186803837976	16.118610561472018	31.453213185213087
2	24.890550077809735	25.271187669867416	32.11763844861687	17.720623803705976
3	20.232766293783865	28.90410553215061	30.64354131207744	20.21958686198809
4	23.47553273686819	33.582058927052245	22.16512427584778	20.77728406023179
5	25.95945470300507	34.655540839747005	21.902802504498993	17.482201952748934
6	19.45000775480559	39.12098800958356	21.903936442327797	19.525067793283053
7	20.79585488818999	20.679694795441353	37.47014067557939	21.054309640789263
8	21.738369058466915	23.31948960004559	28.393458482963972	26.54868285852352
9	23.936351778915853	22.828274378396465	28.204597607000743	25.03077623568694
10-14	23.35020893116017	28.692710075689725	25.47091690378982	22.486164089360283
15-19	23.45809169312037	27.594457644182274	26.860440996313496	22.08700966638386
20-24	23.305427940244716	27.750710954096952	26.73601354146063	22.207847564197703
25-29	23.462012708909356	27.35320634835717	26.71932928698903	22.465451655744452
30-34	23.27442616307754	27.129028916411503	26.93239163604135	22.664153284469613
35-39	23.591295743695824	26.924238083081836	26.868862042299284	22.61560413092305
40-44	23.72132227617957	26.6583541685756	26.946307088473816	22.67401646677101
45-49	23.639886433426593	26.482880404781746	27.08479454168019	22.792438620111472
50-54	23.780396698803766	26.4814507293214	26.866541831049577	22.87161074082526
55-59	23.888158175106085	26.42107207051316	26.927906558870852	22.762863195509905
60-64	23.632414904611736	26.423020948276072	26.965813311300323	22.978750835811866
65-69	23.76308441722698	26.37210755512454	26.9551360197885	22.909672007859974
70-74	23.953923246282073	26.343651115325468	26.707799672977316	22.994625965415143
75-79	23.80667830399785	26.43848403893203	26.941772167882277	22.81306548918784
80-84	24.114257070933085	26.41527777281651	26.644573293379953	22.82589186287045
85-89	23.907210822801073	26.502769591226627	26.844424643683922	22.74559494228838
90-94	23.903742551419928	26.316857784341973	26.841235495460637	22.938164168777465
95-99	23.871032921324485	26.528675269011202	26.886260856711235	22.714030952953078
100-104	24.14361733810047	26.294426583423725	26.832586831185402	22.729369247290407
105-109	23.92392332187793	26.496039898661678	26.817888837042503	22.76214794241789
110-114	24.01716042528733	26.611409973186735	26.668956694955853	22.702472906570087
115-119	24.106204235795303	26.634948750038724	26.81981052372236	22.439036490443616
120-124	24.02808194956228	26.713433641002926	26.989067759389524	22.269416650045272
125-129	24.067137592846873	26.792378974150953	26.810441399214486	22.33004203378769
130-134	24.23830737723337	26.786513234217473	26.77740351829756	22.197775870251593
135-139	24.039151542351927	26.87954869506641	26.94915669458333	22.13214306799833
140-144	24.074480359490693	26.97824093761456	26.83100264111981	22.11627606177494
145-149	24.165550104808254	27.484583637551268	26.402707262028606	21.94715899561187
150	23.99177766311945	27.529381242940403	26.3133803750003	22.165460718939844
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	0.5
4	0.5
5	0.5
6	0.5
7	1.5
8	2.5
9	4.0
10	5.5
11	7.0
12	12.5
13	16.5
14	25.5
15	40.0
16	66.0
17	109.5
18	217.5
19	380.0
20	594.0
21	953.5
22	1460.0
23	2254.0
24	3660.5
25	5599.5
26	8508.0
27	13267.0
28	19600.0
29	27933.5
30	39764.5
31	55790.5
32	80216.0
33	110011.5
34	145508.0
35	202923.0
36	286030.0
37	382984.0
38	499626.0
39	650761.5
40	834140.5
41	1024972.5
42	1215146.0
43	1395320.5
44	1530461.5
45	1610924.5
46	1630328.5
47	1590675.5
48	1528766.0
49	1462127.0
50	1345575.5
51	1183694.5
52	1012952.5
53	844156.0
54	696464.0
55	578613.5
56	478446.0
57	396736.5
58	331637.5
59	271671.0
60	207505.5
61	141423.5
62	88446.5
63	54541.5
64	34009.0
65	17968.5
66	10682.5
67	6137.0
68	3734.0
69	2587.0
70	1803.0
71	1443.5
72	1241.0
73	1060.5
74	834.0
75	493.0
76	162.0
77	76.5
78	38.5
79	19.0
80	13.0
81	9.5
82	7.5
83	4.5
84	1.0
85	2.5
86	2.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	4.153618420533429E-6
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.10238004827667617
100-104	0.0
105-109	0.0
110-114	0.0
115-119	8.307236841066858E-7
120-124	0.0
125-129	0.0
130-134	0.0
135-139	8.307236841066858E-7
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.4075394E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.02232404363171
#Duplication Level	Percentage of total
1	21.95894834136763
2	9.910886581107722
3	6.185795857819258
4	4.368956144998515
5	3.308624052364071
6	2.582161034862363
7	2.125328428644225
8	1.8662542419449126
9	1.5447825901437626
>10	18.28092184116871
>50	6.28116275598294
>100	12.996024084825988
>500	3.7307440921818578
>1k	4.159476381442131
>5k	0.5115526213686956
>10k+	0.18838094977734587
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.9490690785787346E-4	4.153618420533429E-6	0.0	0.0	0.0016656009866339052	0.0
2	0.0014329983550840331	1.2460855261600288E-5	0.0	0.0	0.009042427301501276	0.0
3	0.002076809210266715	1.2460855261600288E-5	0.0	0.0	0.012448394406338688	0.0
4	0.005482776315104127	1.2460855261600288E-5	0.0	0.0	0.017482579932025205	0.0
5	0.005648921051925463	1.2460855261600288E-5	0.0	0.0	0.021187607563141024	0.0
6	0.006899160196506026	1.2460855261600288E-5	0.0	0.0	0.028165686509637185	0.0
7	0.008934433222567406	1.6614473682133716E-5	4.153618420533429E-6	0.0	0.0390273986793321	0.0
8	0.014965487169181946	1.6614473682133716E-5	4.153618420533429E-6	0.0	0.05336153584859297	0.0
9	0.01549299670858969	1.6614473682133716E-5	4.153618420533429E-6	0.0	0.06322222597893933	0.0
10-11	0.016051658386151437	1.6614473682133716E-5	4.153618420533429E-6	0.0	0.10548737021707724	0.0
12-13	0.01660201282687212	1.6614473682133716E-5	4.153618420533429E-6	0.0	0.12646937366840186	0.0
14-15	0.018952960852894038	2.4921710523200576E-5	4.153618420533429E-6	0.0	0.1360829235027265	0.0
16-17	0.021602969405194364	2.4921710523200576E-5	8.307236841066858E-6	0.0	0.1426331797519077	0.0
18-19	0.027436726476833567	2.699851973346729E-5	8.307236841066858E-6	0.0	0.1491045172510988	0.0
20-21	0.03286758256168103	2.9075328943734005E-5	8.307236841066858E-6	0.0	0.15373580178999355	0.0
22-23	0.03512507417324094	3.322894736426743E-5	8.307236841066858E-6	0.0	0.15860799619727928	0.0
24-25	0.03974805147529465	3.5305756574534143E-5	8.307236841066858E-6	0.0	0.1629236057362135	0.0
26-27	0.043527844237980076	3.738256578480086E-5	8.307236841066858E-6	0.0	0.1663358032686817	0.0
28-29	0.05293994357890883	3.738256578480086E-5	8.307236841066858E-6	0.0	0.16923502892621406	6.230427630800143E-6
30-31	0.0736561154513193	4.153618420533429E-5	1.4537664471867001E-5	0.0	0.17241670063634265	8.307236841066858E-6
32-33	0.09177419900168612	5.399703946693458E-5	1.6614473682133716E-5	0.0	0.17506047876101216	8.307236841066858E-6
34-35	0.10630978666434285	5.399703946693458E-5	1.6614473682133716E-5	0.0	0.17788078566855436	8.307236841066858E-6
36-37	0.10876665196008839	5.815065788746801E-5	1.6614473682133716E-5	0.0	0.1797457603393739	8.307236841066858E-6
38-39	0.1109556088677095	6.022746709773472E-5	1.6614473682133716E-5	0.0	0.18125767744444804	8.307236841066858E-6
40-41	0.11232007251885473	6.645789472853486E-5	1.6614473682133716E-5	0.0	0.18278620902320436	8.307236841066858E-6
42-43	0.11257967367013808	6.853470393880158E-5	1.6614473682133716E-5	0.0	0.18434796954932492	8.307236841066858E-6
44-45	0.11328371199241849	7.2688322359335E-5	1.6614473682133716E-5	0.0	0.18563351445048004	8.307236841066858E-6
46-47	0.11460871626856865	7.476513156960172E-5	1.6614473682133716E-5	0.0	0.1869065984963735	1.0384046051333573E-5
48-49	0.11564919768291226	7.476513156960172E-5	1.6614473682133716E-5	0.0	0.1880696116541229	2.0768092102667146E-5
50-51	0.11643007794597256	8.7225986831202E-5	1.6614473682133716E-5	0.0	0.18930738994344185	2.0768092102667146E-5
52-53	0.11722757268271497	9.345641446200216E-5	1.6614473682133716E-5	0.0	0.19038317711435998	2.0768092102667146E-5
54-55	0.11769693156423525	9.96868420928023E-5	1.6614473682133716E-5	0.0	0.19144234981159602	2.4921710523200576E-5
56-57	0.11851934801150087	1.0384046051333573E-4	2.0768092102667146E-5	0.0	0.19263443829828913	2.4921710523200576E-5
58-59	0.11868133912990167	1.121476973544026E-4	2.4921710523200576E-5	0.0	0.19374968484420235	2.4921710523200576E-5
60-61	0.11889940409697969	1.1630131577493602E-4	2.4921710523200576E-5	0.0	0.19452018106121127	2.4921710523200576E-5
62-63	0.11911954587326795	1.2253174340573616E-4	2.4921710523200576E-5	0.0	0.19546512925188264	2.4921710523200576E-5
64-65	0.11915692843905275	1.3083898024680304E-4	2.4921710523200576E-5	0.0	0.1963934629688719	2.4921710523200576E-5
66-67	0.11925246166272502	1.4745345392893673E-4	2.4921710523200576E-5	0.0	0.1971141157648344	2.4921710523200576E-5
68-69	0.11931476593903302	1.4953026313920345E-4	2.4921710523200576E-5	0.0	0.1979136873107871	2.699851973346729E-5
70-71	0.11941029916270529	1.5991430919053704E-4	2.4921710523200576E-5	0.0	0.19865718500806256	2.9075328943734005E-5
72-73	0.11945391215612089	1.6822154603160388E-4	2.4921710523200576E-5	0.0	0.1992116930672038	3.322894736426743E-5
74-75	0.11947468024822355	1.7237516445213732E-4	2.4921710523200576E-5	0.0	0.19999465013947434	3.322894736426743E-5
76-77	0.1195037555771673	1.7860559208293745E-4	2.9075328943734005E-5	0.0	0.20079214487621677	3.322894736426743E-5
78-79	0.11956605985347529	1.9314325655480448E-4	3.322894736426743E-5	0.0	0.20178070606030374	3.322894736426743E-5
80-81	0.1196159032745217	2.118345394472049E-4	3.945937499506758E-5	0.0	0.2026363514549336	3.5305756574534143E-5
82-83	0.1196470554126757	2.2014177628827176E-4	4.568980262586772E-5	0.0	0.20348576642193272	3.738256578480086E-5
84-85	0.11967197712319891	2.3052582233960533E-4	4.568980262586772E-5	0.0	0.2043164901060394	3.738256578480086E-5
86-87	0.1196968988337221	2.3260263154987204E-4	4.568980262586772E-5	6.230427630800143E-6	0.20504752694805328	3.738256578480086E-5
88-89	0.1197155901166145	2.3260263154987204E-4	4.984342104640115E-5	8.307236841066858E-6	0.2060132432308273	3.738256578480086E-5
90-91	0.11973843501792744	2.3675624997040548E-4	5.399703946693458E-5	8.307236841066858E-6	0.20663420918469705	3.945937499506758E-5
92-93	0.11974466544555823	2.3675624997040548E-4	5.399703946693458E-5	8.307236841066858E-6	0.20727594323066945	4.568980262586772E-5
94-95	0.1197778943929225	2.3675624997040548E-4	6.230427630800144E-5	8.307236841066858E-6	0.2080028264542628	4.984342104640115E-5
96-97	0.11978412482055331	2.3675624997040548E-4	6.230427630800144E-5	8.307236841066858E-6	0.20872140244101509	5.192023025666786E-5
98-99	0.11980696972186623	2.409098683909389E-4	6.230427630800144E-5	8.307236841066858E-6	0.20935482925014642	6.022746709773472E-5
100-101	0.1198277378139689	2.429866776012056E-4	6.438108551826816E-5	8.307236841066858E-6	0.2100194081974318	6.645789472853488E-5
102-103	0.11984435228765103	2.450634868114723E-4	6.645789472853486E-5	8.307236841066858E-6	0.21062168286840913	7.476513156960172E-5
104-105	0.11987342761659477	2.450634868114723E-4	6.645789472853486E-5	8.307236841066858E-6	0.2112862618156945	8.099555920040188E-5
106-107	0.11989419570869744	2.512939144422725E-4	6.645789472853486E-5	8.307236841066858E-6	0.21178261921694824	8.930279604146872E-5
108-109	0.1199211942284309	2.533707236525392E-4	7.06115131490683E-5	8.307236841066858E-6	0.21232466642082784	9.96868420928023E-5
110-111	0.11995442317579516	2.596011512833393E-4	7.06115131490683E-5	8.307236841066858E-6	0.21293317151943597	1.0176365130306901E-4
112-113	0.11996896084026704	2.6583157891413946E-4	7.06115131490683E-5	8.307236841066858E-6	0.21360813451277266	1.1630131577493602E-4
114-115	0.11997934488631837	2.6583157891413946E-4	7.06115131490683E-5	8.307236841066858E-6	0.21421456280217055	1.2460855261600288E-4
116-117	0.12000011297842103	2.6583157891413946E-4	7.06115131490683E-5	8.307236841066858E-6	0.21479399257183496	1.2876217103653632E-4
118-119	0.12001257383368263	2.679083881244062E-4	7.06115131490683E-5	8.307236841066858E-6	0.21541288171649442	1.3706940787760316E-4
120-121	0.12001880426131344	2.699851973346729E-4	7.06115131490683E-5	8.307236841066858E-6	0.21605461576246687	1.4745345392893673E-4
122-123	0.12002295787973397	2.720620065449396E-4	7.2688322359335E-5	8.307236841066858E-6	0.21658420211108487	1.6199111840080376E-4
124-125	0.12002918830736478	2.7413881575520633E-4	8.307236841066858E-5	8.307236841066858E-6	0.21732146938072955	1.6822154603160388E-4
126-127	0.12003957235341611	2.7829243417573977E-4	8.307236841066858E-5	8.307236841066858E-6	0.2179922787556457	1.7652878287267073E-4
128-129	0.1200458027810469	2.8659967101680664E-4	8.307236841066858E-5	8.307236841066858E-6	0.21852601872268423	1.848360197137376E-4
130-131	0.12004995639946744	2.9490690785787346E-4	8.7225986831202E-5	8.307236841066858E-6	0.2192529019462776	1.9106644734453776E-4
132-133	0.12007072449157011	2.9490690785787346E-4	9.137960525173544E-5	1.2460855261600288E-5	0.2198198708606804	1.9106644734453776E-4
134-135	0.12009149258367277	2.9490690785787346E-4	9.137960525173544E-5	1.2460855261600288E-5	0.22037645572903192	2.0560411181640473E-4
136-137	0.12012679834024731	2.969837170681402E-4	9.137960525173544E-5	1.6614473682133716E-5	0.22088735079475752	2.118345394472049E-4
138-139	0.12014964324156024	2.990605262784069E-4	9.137960525173544E-5	1.869128289240043E-5	0.2214937790841554	2.139113486574716E-4
>>END_MODULE
