##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.2d.R.1.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24147199
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	76-150
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.499879675485346	37.0	35.0	39.0	33.0	39.0
2	35.68297730929372	36.0	34.0	39.0	33.0	39.0
3	35.95307501296527	37.0	35.0	39.0	33.0	39.0
4	35.95621554284619	37.0	35.0	39.0	33.0	39.0
5	35.74886126544118	36.0	35.0	38.0	33.0	39.0
6	35.61165727751695	36.0	35.0	38.0	33.0	39.0
7	35.46272306779763	35.0	34.0	37.0	33.0	39.0
8	35.74829552694704	36.0	35.0	38.0	33.0	39.0
9	35.72096183909363	36.0	35.0	38.0	33.0	39.0
10-14	35.740112499176405	36.0	35.0	38.0	33.0	39.0
15-19	35.73874364475979	36.0	35.0	38.0	33.0	39.0
20-24	35.723920219483844	36.0	35.0	38.0	33.0	39.0
25-29	35.71346564874874	36.0	35.0	38.0	33.0	39.0
30-34	35.70622246497409	36.0	35.0	38.0	33.0	39.0
35-39	35.69337437439431	36.0	35.0	38.0	33.0	39.0
40-44	35.67735866176445	36.0	35.0	38.0	33.0	39.0
45-49	35.66667556763001	36.0	35.0	38.0	33.0	39.0
50-54	35.64484590531597	36.0	35.0	38.0	33.0	39.0
55-59	35.62600600591398	36.0	35.0	38.0	33.0	39.0
60-64	35.61477613200604	36.0	35.0	38.0	33.0	39.0
65-69	35.594985430815385	36.0	35.0	38.0	33.0	39.0
70-74	35.57610010171366	36.0	35.0	38.0	33.0	39.0
75-79	35.55695665971068	36.0	35.0	38.0	33.0	39.0
80-84	35.534874293267755	36.0	34.0	38.0	33.0	39.0
85-89	35.51573356858158	36.0	34.0	38.0	33.0	39.0
90-94	35.49725577481842	36.0	34.0	38.0	33.0	39.0
95-99	35.470371605247195	36.0	34.0	38.0	33.0	39.0
100-104	35.435411339924805	36.0	34.0	38.0	32.0	39.0
105-109	35.40846895950286	36.0	34.0	38.0	32.0	39.0
110-114	35.380389090621165	36.0	34.0	38.0	32.0	39.0
115-119	35.34902158396248	36.0	34.0	38.0	32.0	39.0
120-124	35.319316733568016	36.0	34.0	38.0	32.0	39.0
125-129	35.283850810874	36.0	34.0	38.0	32.0	39.0
130-134	35.248547843724126	36.0	34.0	38.0	32.0	39.0
135-139	35.209552831149345	36.0	34.0	38.0	32.0	39.0
140-144	35.174645767872974	36.0	34.0	38.0	32.0	39.0
145-149	35.165351383028	36.0	34.0	38.0	32.0	39.0
150	35.66697479868561	36.0	34.0	38.0	33.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	8.0
22	158.0
23	1618.0
24	11375.0
25	43398.0
26	105955.0
27	190183.0
28	279742.0
29	364817.0
30	459822.0
31	588189.0
32	783828.0
33	1112156.0
34	1756144.0
35	3372415.0
36	1.1873242E7
37	3204118.0
38	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.17026968635162	10.746240174688584	14.306818774301732	38.77667136465807
2	22.44755981206416	12.885150094356785	36.819782287594116	27.84750780598494
3	21.088945736585636	17.80030804435401	28.15109363531266	32.9596525837477
4	26.230792466494822	25.83711893975467	22.117481612542026	25.814606981208478
5	25.61288518055647	31.443900990185934	24.28831654144985	18.654897287807746
6	19.064798030775368	37.10967525841228	25.621931084343675	18.203595626468676
7	15.881436063698384	25.07664457961002	40.383483384018355	18.65843597267324
8	18.437706856418536	24.638024044542835	31.44739775867861	25.47687134036002
9	19.535111606444016	23.337983429281074	32.63910573047643	24.487799233798476
10-14	20.202415513449917	29.750879241356408	26.8443983324132	23.202306912780475
15-19	20.401426163948887	28.41142052948944	27.59061807339918	23.596535233162495
20-24	20.557569414597197	28.610139527468792	27.263901447030996	23.568389610903015
25-29	20.538802062003576	28.819984723476004	27.146874789131136	23.49433842538928
30-34	20.54945360671549	28.51112421614185	27.17030320778569	23.769118969356963
35-39	20.624034057337447	28.521666742341612	27.177028670711323	23.677270529609615
40-44	20.708257780598853	28.611450801509857	27.044359136752178	23.635932281139112
45-49	20.793656814943333	28.381119862284155	27.02489639340721	23.8003269293653
50-54	20.92659026929441	28.36122677742472	27.08628256653539	23.625900386745474
55-59	20.946588583497075	28.41415057783816	26.994112984266707	23.64514785439806
60-64	20.89676602085105	28.34061298707632	26.965593477502694	23.797027514569937
65-69	21.00409323073268	28.260301940070224	27.04466875869114	23.69093607050595
70-74	21.117673372509888	28.173999379421065	27.036720529824628	23.671606718244416
75-79	21.136066767163822	28.086142931841994	27.01794404633537	23.759846254658807
80-84	21.18460080018733	28.07161108102152	27.01443933812202	23.72934878066913
85-89	21.177723428153687	28.078159959791137	27.0522221125887	23.691894499466475
90-94	21.170176751761478	27.952540581544184	27.00345425367771	23.87382841301663
95-99	21.262647454713367	27.866456365565313	27.120262159271704	23.750634020449617
100-104	21.327686795490383	27.915454530741656	27.00567196538554	23.751186708382413
105-109	21.30274412966276	27.74468190559834	27.107261561399522	23.845312403339385
110-114	21.408926543098612	27.698705892060193	27.11611074493557	23.776256819905626
115-119	21.45340622713358	27.750237762715958	27.074542230985227	23.721813779165235
120-124	21.42779267422622	27.657456132805596	27.056866085848885	23.857885107119294
125-129	21.456676406472504	27.565120159814406	27.135775983383496	23.84242745032959
130-134	21.551734548695002	27.677969512289362	27.00509553171501	23.76520040730062
135-139	21.5445219534523	27.535885069769826	27.111763319809423	23.807829656968448
140-144	21.621182868605455	27.438397806266984	27.21233591862392	23.728083406503643
145-149	21.26625459246073	27.419705166580727	27.385840936367998	23.928199304590546
150	21.973993481021274	25.63733885534164	27.62404874171774	24.764618921919347
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.5
9	3.0
10	2.5
11	1.0
12	4.0
13	12.0
14	31.0
15	69.5
16	139.0
17	339.0
18	659.5
19	1178.0
20	2203.0
21	3749.0
22	5949.0
23	9717.0
24	15705.5
25	24628.0
26	36741.0
27	51645.5
28	69960.5
29	96815.0
30	129795.5
31	164833.0
32	199991.0
33	237467.0
34	278315.0
35	325501.5
36	396185.0
37	493877.5
38	611686.5
39	758265.0
40	925350.0
41	1088083.0
42	1257404.0
43	1433275.0
44	1575317.0
45	1662554.5
46	1686324.0
47	1639921.5
48	1543886.5
49	1414305.5
50	1249600.5
51	1068486.5
52	888468.5
53	710974.0
54	548391.0
55	411473.5
56	304897.0
57	229015.0
58	172258.5
59	125752.0
60	88413.5
61	62723.0
62	45277.0
63	30462.5
64	20225.0
65	14636.5
66	12243.0
67	9121.5
68	5027.0
69	2879.5
70	2241.5
71	1383.5
72	663.0
73	299.5
74	160.0
75	98.0
76	49.0
77	36.0
78	23.0
79	10.5
80	8.0
81	5.5
82	3.0
83	2.5
84	1.5
85	1.0
86	0.5
87	0.0
88	0.5
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	9.11078754931369E-5
3	1.1181421083248622E-4
4	1.3666181323970537E-4
5	7.040154015378762E-5
6	1.242380120360958E-4
7	1.0353167669674648E-4
8	6.21190060180479E-5
9	1.1181421083248622E-4
10-14	7.537106063523144E-5
15-19	3.039690027816477E-4
20-24	0.019991552643434957
25-29	3.4289691321962436E-4
30-34	2.8326266744229837E-4
35-39	2.559303047943573E-4
40-44	1.9629605901703135E-4
45-49	3.602902349046778E-4
50-54	4.273787614041695E-4
55-59	3.801683168304531E-4
60-64	3.3047311201601476E-4
65-69	2.600715718622271E-4
70-74	2.865756810965943E-4
75-79	1.8967004741792597E-4
80-84	1.8387232634521863E-4
85-89	2.5178927129724973E-4
90-94	2.3108314068260436E-4
95-99	1.3914699759665802E-4
100-104	1.4328855262369413E-4
105-109	1.532279996025017E-4
110-114	1.615112322738006E-4
115-119	2.153493103305814E-4
120-124	1.7393708064649896E-4
125-129	1.772517167698462E-4
130-134	1.54061932300416E-4
135-139	1.9713994503324175E-4
140-144	1.7068186501952812E-4
145-149	1.2133775992581006E-4
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
76-77	3.0
78-79	2.0
80-81	4.0
82-83	5.0
84-85	4.0
86-87	7.0
88-89	10.0
90-91	11.0
92-93	11.0
94-95	8.0
96-97	19.0
98-99	16.0
100-101	19.0
102-103	22.0
104-105	20.0
106-107	29.0
108-109	39.0
110-111	45.0
112-113	39.0
114-115	50.0
116-117	38.0
118-119	61.0
120-121	63.0
122-123	99.0
124-125	77.0
126-127	86.0
128-129	114.0
130-131	148.0
132-133	210.0
134-135	277.0
136-137	569.0
138-139	1975.0
140-141	4343.0
142-143	5878.0
144-145	61483.0
146-147	744238.0
148-149	674549.0
150-151	2.2652628E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.2635882798931
#Duplication Level	Percentage of total
1	30.773687585782174
2	11.013277285824055
3	6.975478485939466
4	5.148526463131533
5	3.8803257265424467
6	3.021696992307052
7	2.4551001273278263
8	2.1134644193550263
9	1.8102507468736477
>10	21.192699487611655
>50	5.478554213721135
>100	5.43505268716489
>500	0.4111387110266941
>1k	0.2907470673923988
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.141267067869859E-6	1.2423801203609579E-5	0.0	0.0	1.946395521898834E-4	8.282534135739719E-6
2	1.2423801203609579E-5	7.868407428952732E-5	0.0	0.0	0.002203154080106765	3.3130136542958875E-5
3	1.2423801203609579E-5	9.110787549313691E-5	0.0	4.141267067869859E-6	0.003321296188431627	8.696660842526704E-5
4	3.3130136542958875E-5	9.110787549313691E-5	0.0	4.141267067869859E-6	0.0050274982203940095	1.2009674496822593E-4
5	3.3130136542958875E-5	9.524914256100676E-5	0.0	4.141267067869859E-6	0.006327856079705145	1.325205461718355E-4
6	6.626027308591775E-5	9.524914256100676E-5	0.0	4.141267067869859E-6	0.007429433119758528	1.408030803075752E-4
7	9.524914256100676E-5	1.4494434737544507E-4	0.0	4.141267067869859E-6	0.008547575228083389	1.4908561444331494E-4
8	9.939040962887663E-5	1.4908561444331494E-4	0.0	4.141267067869859E-6	0.010854260984886902	1.532268815111848E-4
9	1.1595547790035606E-4	1.4908561444331494E-4	0.0	4.141267067869859E-6	0.014788464699363268	1.6150941564692451E-4
10-11	1.7807448391840395E-4	1.739332168505341E-4	0.0	4.141267067869859E-6	0.019776620882612513	2.2776968873284225E-4
12-13	2.298403222667772E-4	1.7807448391840395E-4	0.0	4.141267067869859E-6	0.02404626722958634	2.9402996181876E-4
14-15	2.4019348993645185E-4	1.884276515880786E-4	0.0	4.141267067869859E-6	0.028997152009224755	3.3958389956532844E-4
16-17	2.6918235941154087E-4	2.153458875292327E-4	0.0	4.141267067869859E-6	0.033714055199528525	3.437251666331983E-4
18-19	2.8781806121695524E-4	2.4019348993645185E-4	0.0	4.141267067869859E-6	0.037826333397923295	3.4579580016713327E-4
20-21	3.54078334302873E-4	3.002418624205648E-4	0.0	4.141267067869859E-6	0.04185371562142673	3.54078334302873E-4
22-23	4.369036756602702E-4	3.1680693069204425E-4	0.0	4.141267067869859E-6	0.04636562609187095	3.9756163851550647E-4
24-25	5.052345822801228E-4	3.3130136542958876E-4	0.0	8.282534135739719E-6	0.05141797191467218	4.058441726512462E-4
26-27	5.694242218321057E-4	3.3544263249745863E-4	0.0	1.6565068271479437E-5	0.056292243253555	4.4311557626207495E-4
28-29	5.984130913071947E-4	3.3544263249745863E-4	0.0	1.6565068271479437E-5	0.061069194816342875	4.679631786692941E-4
30-31	6.253313272483488E-4	3.3544263249745863E-4	0.0	1.6565068271479437E-5	0.06597659629176866	4.969520481443832E-4
32-33	6.667439979270473E-4	3.4165453309926335E-4	0.0	2.0706335339349298E-5	0.07020275933452985	5.61141687696366E-4
34-35	7.185098362754206E-4	3.437251666331983E-4	0.0	2.4847602407219158E-5	0.07381808548478025	5.963424577732598E-4
36-37	7.433574386826397E-4	3.54078334302873E-4	0.0	2.4847602407219158E-5	0.07743548226856456	6.17048793112609E-4
38-39	7.619931404880541E-4	3.851378373118969E-4	0.0	3.3130136542958875E-5	0.08152084223101819	6.274019607822837E-4
40-41	8.158296123703622E-4	3.892791043797668E-4	0.0	3.3130136542958875E-5	0.08551095305091079	6.315432278501535E-4
42-43	8.365359477097116E-4	3.892791043797668E-4	0.0	3.3130136542958875E-5	0.08903724195920198	6.418963955198282E-4
44-45	8.84160518990215E-4	3.9756163851550647E-4	0.0	3.3130136542958875E-5	0.09354501116257832	6.418963955198282E-4
46-47	9.400676244064581E-4	4.0998543971911607E-4	0.0	3.3130136542958875E-5	0.09770905519932147	6.584614637913076E-4
48-49	9.752683944833519E-4	4.2033860738879077E-4	0.0	3.3130136542958875E-5	0.10167638905034079	6.750265320627871E-4
50-51	9.897628292208963E-4	4.3069177505846535E-4	0.0	3.3130136542958875E-5	0.10602471947160413	6.833090661985268E-4
52-53	0.0010415286675692697	4.327624085924003E-4	0.0	3.3130136542958875E-5	0.1098760978447231	6.853796997324617E-4
54-55	0.0010725881705782934	4.348330421263352E-4	0.0	3.3130136542958875E-5	0.11314562819480636	6.874503332663966E-4
56-57	0.001116071474790927	4.389743091942051E-4	0.0	3.934203714476367E-5	0.11760991409397006	6.998741344700062E-4
58-59	0.0011305659095284717	4.5761001099961946E-4	0.0	5.383647188230817E-5	0.12213631899915182	7.143685692075507E-4
60-61	0.0011678373131393002	4.679631786692941E-4	0.0	5.383647188230817E-5	0.12619061945859641	7.247217368772254E-4
62-63	0.0012196031514876736	4.803869798729037E-4	0.0	5.383647188230817E-5	0.12992811298734896	7.247217368772254E-4
64-65	0.0012444507538948927	4.886695140086434E-4	0.0	5.383647188230817E-5	0.13496803500894658	7.247217368772254E-4
66-67	0.0012775808904378517	5.052345822801228E-4	0.0	5.383647188230817E-5	0.1393205066972778	7.288630039450953E-4
68-69	0.0013417705299898345	5.280115511534071E-4	0.0	5.383647188230817E-5	0.14237054989276396	7.454280722165748E-4
70-71	0.001416313337211492	5.507885200266913E-4	0.0	5.383647188230817E-5	0.14532948521275696	7.557812398862494E-4
72-73	0.0014494434737544507	5.59071054162431E-4	0.0	5.5907105416243096E-5	0.14847063628373625	7.682050410898589E-4
74-75	0.0015115624797724988	5.673535882981707E-4	0.0	5.797773895017803E-5	0.15204454976330795	7.744169416916637E-4
76-77	0.001532268815111848	5.756361224339105E-4	0.0	6.004837248411296E-5	0.15476950349396632	7.826994758274035E-4
78-79	0.0015798933863923515	5.839186565696501E-4	0.0	8.903724195920198E-5	0.1574116318832673	7.930526434970781E-4
80-81	0.0016378711253425293	5.8805992363752E-4	0.0	9.110787549313691E-5	0.16069772730162202	7.95123277031013E-4
82-83	0.0016792837960212278	5.922011907053898E-4	4.141267067869859E-6	9.110787549313691E-5	0.16379332443485473	7.97193910564948E-4
84-85	0.0017496853361750157	5.963424577732598E-4	4.141267067869859E-6	9.110787549313691E-5	0.1662863672097124	8.034058111667527E-4
86-87	0.0018532170128717621	5.963424577732598E-4	4.141267067869859E-6	9.524914256100676E-5	0.16844603798560653	8.034058111667527E-4
88-89	0.0019070534847540704	5.963424577732598E-4	4.141267067869859E-6	9.73197760949417E-5	0.17056636672435588	8.034058111667527E-4
90-91	0.001940183621297029	6.149781595786741E-4	4.141267067869859E-6	9.939040962887663E-5	0.1735605028144258	8.034058111667527E-4
92-93	0.001973313757839988	6.232606937144139E-4	4.141267067869859E-6	9.939040962887663E-5	0.17649044926494373	8.116883453024924E-4
94-95	0.0020623509997991897	6.253313272483488E-4	4.141267067869859E-6	9.939040962887663E-5	0.17961503526765155	8.241121465061021E-4
96-97	0.002184518378301351	6.253313272483488E-4	4.141267067869859E-6	9.939040962887663E-5	0.182050100303559	8.26182780040037E-4
98-99	0.002263202452590878	6.274019607822837E-4	4.141267067869859E-6	9.939040962887663E-5	0.18424290121599612	8.282534135739719E-4
100-101	0.0023460277939482757	6.294725943162186E-4	4.141267067869859E-6	9.939040962887663E-5	0.18658685837641045	8.282534135739719E-4
102-103	0.0023729460298894294	6.294725943162186E-4	4.141267067869859E-6	1.0146104316281155E-4	0.18857259593545406	8.282534135739719E-4
104-105	0.002422641234703868	6.377551284519583E-4	4.141267067869859E-6	1.0560231023068142E-4	0.1904071772465204	8.282534135739719E-4
106-107	0.0025220316443327444	6.48108296121633E-4	4.141267067869859E-6	1.0974357729855127E-4	0.19216514511683114	8.344653141757767E-4
108-109	0.002613139519825881	6.522495631895028E-4	4.141267067869859E-6	1.1595547790035606E-4	0.19405356289977982	8.448184818454513E-4
110-111	0.002698035494717213	6.626027308591775E-4	4.141267067869859E-6	1.1595547790035606E-4	0.1957121403604617	8.489597489133212E-4
112-113	0.002758083867201326	6.626027308591775E-4	4.141267067869859E-6	1.1595547790035606E-4	0.19753843913739227	8.489597489133212E-4
114-115	0.0027974259043460903	6.667439979270473E-4	4.141267067869859E-6	1.2009674496822593E-4	0.19962977900666656	8.489597489133212E-4
116-117	0.002861615543898073	6.708852649949173E-4	4.141267067869859E-6	1.2837927910396566E-4	0.2012220961942625	8.489597489133212E-4
118-119	0.002942370251721535	6.874503332663967E-4	4.141267067869859E-6	1.3666181323970534E-4	0.2028082014812567	8.489597489133212E-4
120-121	0.0031080209344363295	6.998741344700063E-4	4.141267067869859E-6	1.408030803075752E-4	0.2044750614760743	8.489597489133212E-4
122-123	0.0032840247848207986	7.06086035071811E-4	4.141267067869859E-6	1.408030803075752E-4	0.20594314065163416	8.510303824472562E-4
124-125	0.0034082627968568943	7.081566686057459E-4	4.141267067869859E-6	1.408030803075752E-4	0.20733874765350632	8.531010159811911E-4
126-127	0.003447604834001658	7.102273021396808E-4	4.141267067869859E-6	1.408030803075752E-4	0.20872607212124272	8.531010159811911E-4
128-129	0.0035076532064857707	7.164392027414856E-4	4.141267067869859E-6	1.408030803075752E-4	0.21009269025363975	8.55171649515126E-4
130-131	0.0036091142496485823	7.205804698093556E-4	4.141267067869859E-6	1.4494434737544507E-4	0.2115110742243852	8.593129165829958E-4
132-133	0.003712645926345329	7.205804698093556E-4	4.141267067869859E-6	1.532268815111848E-4	0.21271825357466928	8.696660842526704E-4
134-135	0.003907285478535212	7.205804698093556E-4	4.141267067869859E-6	1.532268815111848E-4	0.21392543292495333	8.717367177866055E-4
136-137	0.004120560732530511	7.247217368772254E-4	4.141267067869859E-6	1.532268815111848E-4	0.21508291707042293	8.758779848544753E-4
138-139	0.00414126706786986	7.330042710129651E-4	4.141267067869859E-6	1.552975150451197E-4	0.2161762115763406	8.800192519223451E-4
>>END_MODULE
