##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.2d.R.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24147199
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	65-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.62902417791811	37.0	35.0	39.0	34.0	39.0
2	35.35028149641704	36.0	34.0	38.0	33.0	39.0
3	35.62066942008471	36.0	35.0	38.0	33.0	39.0
4	35.663009196221886	36.0	35.0	38.0	33.0	39.0
5	35.67832964808879	36.0	35.0	38.0	33.0	39.0
6	35.545854862918056	36.0	35.0	38.0	33.0	39.0
7	35.56184682124001	35.0	35.0	38.0	33.0	39.0
8	35.69813786683913	36.0	35.0	38.0	33.0	39.0
9	35.73000367454627	36.0	35.0	38.0	33.0	39.0
10-14	35.588446651721384	36.2	35.0	38.0	33.0	39.0
15-19	35.53350927368429	36.0	35.0	38.0	33.0	39.0
20-24	35.4935115580072	36.0	35.0	38.0	32.8	39.0
25-29	35.4751970114629	36.0	35.0	38.0	33.0	39.0
30-34	35.44320073727806	36.0	35.0	38.0	32.6	39.0
35-39	35.43570034768836	36.0	35.0	38.0	32.6	39.0
40-44	35.394758539075276	36.0	34.8	38.0	32.2	39.0
45-49	35.338590682919374	36.0	34.0	38.0	32.0	39.0
50-54	35.3361143046032	36.0	34.0	38.0	32.0	39.0
55-59	35.35345540491052	36.0	34.2	38.0	32.0	39.0
60-64	35.29202408941923	36.0	34.0	38.0	32.0	39.0
65-69	35.26669799062266	36.0	34.0	38.0	32.0	39.0
70-74	35.22531382587316	36.0	34.0	38.0	32.0	39.0
75-79	35.16042126801206	36.0	34.0	38.0	31.8	39.0
80-84	35.10392562020918	36.0	34.0	38.0	31.8	39.0
85-89	35.04882029902514	36.0	34.0	38.0	31.2	39.0
90-94	34.954348235751446	36.0	34.0	38.0	31.0	39.0
95-99	34.83924497368615	36.0	34.0	38.0	30.6	39.0
100-104	34.76969373512124	36.0	34.0	38.0	30.2	39.0
105-109	34.505533473597815	35.6	34.0	38.0	29.6	39.0
110-114	34.35734344854959	35.4	34.0	38.0	29.0	39.0
115-119	34.254456969131546	35.0	34.0	38.0	28.2	39.0
120-124	34.20648523816329	35.0	34.0	38.0	28.2	39.0
125-129	34.170250789607465	35.0	34.0	38.0	28.4	39.0
130-134	34.097827521348634	35.0	34.0	38.0	28.0	39.0
135-139	33.97688196796143	35.0	33.8	38.0	27.6	39.0
140-144	33.822356742182095	35.0	33.4	38.0	26.8	39.0
145-149	33.87716921952018	35.0	33.0	37.8	27.0	39.0
150	35.05943176907596	35.0	34.0	38.0	30.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	10.0
21	185.0
22	1661.0
23	9551.0
24	32466.0
25	74714.0
26	140556.0
27	237817.0
28	363985.0
29	505627.0
30	680664.0
31	907917.0
32	1224159.0
33	1728399.0
34	2593732.0
35	4270484.0
36	9601013.0
37	1774254.0
38	5.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.9056674854918	18.36106953854151	15.779068205798941	31.954194770167753
2	24.162562291386262	25.389387812640297	33.723907273882986	16.724142622090454
3	19.516702537631797	29.06530898262776	30.477659127255297	20.940329352485147
4	23.305725852509852	33.86330646465455	22.677764820673403	20.153202862162193
5	24.91638471194941	34.9321509298035	22.797617230884626	17.353847127362474
6	18.738649563454544	38.4491054221237	23.307162872182403	19.505082142239356
7	20.062783267855757	19.984438774221342	38.67262363111447	21.280154326808436
8	21.400747722643825	22.864570281865934	28.681218339741328	27.053463655748917
9	23.376164448290307	22.904287107824047	28.974552499227137	24.744995944658513
10-14	23.13957429330671	28.67842936896406	25.877035838382678	22.304960499346553
15-19	22.917061312162957	27.82212545645563	27.418844728119396	21.841968503262013
20-24	22.996211693124323	27.9065046012169	27.307343597077242	21.789940108581536
25-29	23.071851107865555	27.866807243357705	27.275043370454682	21.786298278322054
30-34	23.069607369368185	27.630065085395618	27.452948062423303	21.847379482812894
35-39	23.14500181279826	27.6170551095144	27.43887377942985	21.799069298257486
40-44	23.29668795126093	27.403073954871537	27.51001637912538	21.790221714742152
45-49	23.292855622716324	27.242242050516914	27.598741369547664	21.866160957219098
50-54	23.375899622974906	27.244191759052466	27.555857720806458	21.82405089716617
55-59	23.54940628931745	27.13078812991933	27.43823993830506	21.88156564245816
60-64	23.461586774197592	27.108784974263767	27.510819184542434	21.918809066996207
65-69	23.558952790063863	27.03183794869039	27.46934215518287	21.93986710606288
70-74	23.624081565905946	26.993881920955165	27.383114005096214	21.998922508042675
75-79	23.54895662881087	27.047593466378384	27.449689630154833	21.953760274655913
80-84	23.648394899758106	27.087436208669452	27.32562391575962	21.93854497581282
85-89	23.70308730529765	27.004277904820224	27.357198509221913	21.935436280660213
90-94	23.634549455327562	26.94332747255434	27.433382996918194	21.988740075199903
95-99	23.721523782987244	27.086148489848043	27.394918608496894	21.79740911866782
100-104	23.87308839193001	27.077963767956103	27.207749855115175	21.841197984998708
105-109	23.681648439992646	27.219120389849298	27.286217248864435	21.813013921293617
110-114	23.70933857300815	27.23161424734531	27.205709338523302	21.853337841123235
115-119	23.798021891194928	27.248240816150332	27.207497684019437	21.7462396086353
120-124	23.770680672367277	27.155598032855448	27.370022366086218	21.703698928691058
125-129	23.766287538446985	27.245211348821886	27.327201148014606	21.661299964716523
130-134	23.831737991475517	27.181998566257636	27.35728336853472	21.628980073732126
135-139	23.73657735045449	27.1895828057282	27.47933441995852	21.594505423858788
140-144	23.81007717964588	27.327542626032177	27.39187447979812	21.470505714523824
145-149	23.55510865954125	27.186309582289113	27.52739219386577	21.731189564303868
150	24.426274410553	25.588148395384554	27.38924003417266	22.59633715988979
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	0.0
7	1.5
8	3.5
9	4.0
10	4.5
11	5.0
12	8.0
13	14.5
14	25.0
15	44.0
16	70.0
17	121.0
18	196.0
19	334.5
20	542.5
21	836.0
22	1276.0
23	2093.0
24	3458.5
25	5882.0
26	9606.0
27	15793.5
28	28189.5
29	43412.5
30	55862.0
31	72431.0
32	96920.0
33	128511.5
34	173960.5
35	243125.0
36	340888.5
37	459784.0
38	600219.5
39	779297.5
40	988167.5
41	1190892.5
42	1380754.0
43	1559936.5
44	1688668.5
45	1750807.0
46	1758027.0
47	1702791.0
48	1595390.5
49	1452706.5
50	1271440.5
51	1071792.5
52	878434.5
53	700686.0
54	550766.0
55	421926.5
56	315558.0
57	233799.0
58	170319.5
59	123146.0
60	86341.0
61	58434.0
62	39975.5
63	28030.5
64	19486.0
65	13249.0
66	9281.5
67	6987.0
68	6174.5
69	4530.0
70	2229.5
71	1353.0
72	805.0
73	566.0
74	410.0
75	237.5
76	149.0
77	68.5
78	29.5
79	14.5
80	10.0
81	5.5
82	4.0
83	2.0
84	0.5
85	1.5
86	1.0
87	0.5
88	0.5
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	4.141267067869859E-6
8	4.141267067869859E-5
9	1.4080308030757523E-4
10-14	0.007667970102867832
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	8.282534135739719E-7
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	8.282534135739719E-7
65-69	8.282534958944261E-7
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	8.282693840484444E-7
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	2.154002157133745E-5
125-129	0.0
130-134	8.286091151344576E-7
135-139	1.6575610072166146E-6
140-144	8.294035020418297E-7
145-149	3.3860317825471424E-6
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
64-65	1.0
66-67	3.0
68-69	0.0
70-71	9.0
72-73	7.0
74-75	8.0
76-77	20.0
78-79	28.0
80-81	28.0
82-83	42.0
84-85	52.0
86-87	75.0
88-89	95.0
90-91	99.0
92-93	97.0
94-95	158.0
96-97	186.0
98-99	242.0
100-101	306.0
102-103	342.0
104-105	381.0
106-107	437.0
108-109	451.0
110-111	441.0
112-113	453.0
114-115	516.0
116-117	503.0
118-119	526.0
120-121	519.0
122-123	710.0
124-125	674.0
126-127	846.0
128-129	947.0
130-131	1101.0
132-133	1399.0
134-135	2075.0
136-137	2867.0
138-139	5289.0
140-141	10159.0
142-143	15974.0
144-145	83030.0
146-147	814098.0
148-149	1486397.0
150-151	2.1715608E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.69020600711633
#Duplication Level	Percentage of total
1	25.200679339837865
2	11.801119593605911
3	7.738003275823633
4	5.476963000174177
5	4.132321817477965
6	3.429504809740476
7	2.6879380507869133
8	2.236662595218191
9	1.9666832532562049
>10	22.51302512474937
>50	5.445100701715082
>100	5.887609332466686
>500	0.8558826323543816
>1k	0.5738318121805885
>5k	0.05467466061257588
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	1.2423801203609579E-5	0.0	0.0	5.59071054162431E-4	0.0
2	1.6565068271479437E-5	4.5553937746568455E-5	0.0	0.0	0.00469619685496442	0.0
3	3.7271403610828735E-5	4.9695204814438316E-5	0.0	0.0	0.006071097521497214	0.0
4	8.282534135739719E-5	4.9695204814438316E-5	0.0	0.0	0.007284488772383082	0.0
5	8.282534135739719E-5	4.9695204814438316E-5	0.0	0.0	0.008717367177866054	0.0
6	9.110787549313691E-5	4.9695204814438316E-5	0.0	4.141267067869859E-6	0.010266201061249381	0.0
7	9.939040962887663E-5	5.797773895017803E-5	0.0	4.141267067869859E-6	0.01204694590043342	0.0
8	1.5736814857905465E-4	6.21190060180479E-5	0.0	4.141267067869859E-6	0.017157269462184828	0.0
9	1.6979194978266422E-4	6.21190060180479E-5	0.0	4.141267067869859E-6	0.02205638840347487	0.0
10-11	1.863570180541437E-4	6.833090661985267E-5	0.0	4.141267067869859E-6	0.027626392609759833	0.0
12-13	2.443347570043217E-4	8.075470782346226E-5	0.0	6.211900601804789E-6	0.03305973500280508	0.0
14-15	2.8781806121695524E-4	9.73197760949417E-5	0.0	8.282534135739719E-6	0.040333870607518495	0.0
16-17	3.1266566362417433E-4	1.0146104316281155E-4	0.0	8.282534135739719E-6	0.04581276693831032	0.0
18-19	3.5200770076893804E-4	1.0974357729855127E-4	0.0	8.282534135739719E-6	0.05282807335128186	0.0
20-21	3.9134973791370175E-4	1.118142108324862E-4	0.0	8.282534135739719E-6	0.0576795677212914	0.0
22-23	4.2862114152453045E-4	1.263086455700307E-4	0.0	8.282534135739719E-6	0.06403848330400558	0.0
24-25	4.803869798729037E-4	1.408030803075752E-4	0.0	8.282534135739719E-6	0.0685690294762552	0.0
26-27	5.093758493479926E-4	1.5736814857905467E-4	0.0	8.282534135739719E-6	0.07201870494379078	0.0
28-29	5.797773895017803E-4	1.6772131624872931E-4	0.0	8.282534135739719E-6	0.07572928023660219	0.0
30-31	7.474987057505096E-4	1.739332168505341E-4	2.0706335339349297E-6	8.282534135739719E-6	0.08011281142794244	2.0706335339349297E-6
32-33	8.55171649515126E-4	1.801451174523389E-4	4.141267067869859E-6	1.6565068271479437E-5	0.08500985973569854	4.141267067869859E-6
34-35	9.297144567367835E-4	1.9256891865594846E-4	4.141267067869859E-6	1.6565068271479437E-5	0.08772653093222116	4.141267067869859E-6
36-37	9.71127127415482E-4	2.1120462046136284E-4	4.141267067869859E-6	1.8635701805414367E-5	0.09014503089985718	4.141267067869859E-6
38-39	0.0010166810651620504	2.2569905519890734E-4	4.141267067869859E-6	2.0706335339349298E-5	0.09325512246782744	4.141267067869859E-6
40-41	0.0010560231023068142	2.4019348993645185E-4	1.2423801203609579E-5	2.0706335339349298E-5	0.09548726541740929	4.141267067869859E-6
42-43	0.001087082605315838	2.526172911400614E-4	1.2423801203609579E-5	2.2776968873284228E-5	0.09794303678865611	4.141267067869859E-6
44-45	0.0011077889406551874	2.6089982527580113E-4	1.2423801203609579E-5	2.4847602407219158E-5	0.09983559583867263	4.141267067869859E-6
46-47	0.001149201611333886	3.0231249595449975E-4	1.2423801203609579E-5	2.4847602407219158E-5	0.101955924577422	4.141267067869859E-6
48-49	0.0011802611143429099	3.2094819775991407E-4	1.4494434737544507E-5	2.4847602407219158E-5	0.10406797078203564	6.211900601804789E-6
50-51	0.0012092499838179988	3.3130136542958876E-4	1.6565068271479437E-5	2.4847602407219158E-5	0.10597295363325576	8.282534135739719E-6
52-53	0.001238238853293088	3.5200770076893804E-4	1.6565068271479437E-5	2.4847602407219158E-5	0.10791934915515461	8.282534135739719E-6
54-55	0.001298287225777201	3.6650213550648255E-4	1.6565068271479437E-5	2.4847602407219158E-5	0.10966282259072781	1.0353167669674649E-5
56-57	0.0013728300329988582	3.727140361082873E-4	1.6565068271479437E-5	2.4847602407219158E-5	0.11149119200119235	1.2423801203609579E-5
58-59	0.0014349490390169063	3.727140361082873E-4	1.6565068271479437E-5	2.4847602407219158E-5	0.11307729728818651	1.2423801203609579E-5
60-61	0.0015157037468403684	3.892791043797668E-4	1.6565068271479437E-5	2.4847602407219158E-5	0.11431139487441172	1.2423801203609579E-5
62-63	0.0015488338833833273	3.9963227204944143E-4	1.8635701805414367E-5	2.8988869475089015E-5	0.11596790170155967	1.6565068271479437E-5
64-65	0.0015674695851887418	4.1826797385485575E-4	2.0706335339349298E-5	2.8988869475089015E-5	0.11749809988313759	1.6565068271479437E-5
66-67	0.0016730718954194232	4.596806445335544E-4	2.0706335339349298E-5	2.8988869475089015E-5	0.11868043163101442	1.6565068271479437E-5
68-69	0.0016979194978266423	4.803869798729037E-4	2.0706335339349298E-5	3.105950300902395E-5	0.11971160713091403	1.6565068271479437E-5
70-71	0.001733120267903536	4.886695140086434E-4	2.0706335339349298E-5	3.3130136542958875E-5	0.12091257458059629	2.4847602407219158E-5
72-73	0.0017579678703107554	5.01093315212253E-4	2.4847602407219158E-5	3.3130136542958875E-5	0.12181122953432405	3.105950300902395E-5
74-75	0.0017745329385822346	5.30082184687342E-4	2.4847602407219158E-5	3.3130136542958875E-5	0.12273266145692509	3.3130136542958875E-5
76-77	0.001789027373319779	5.818480230357152E-4	2.4847602407219158E-5	3.3130136542958875E-5	0.12376383695682469	3.3130136542958875E-5
78-79	0.001849075745803892	6.108368925108042E-4	2.4847602407219158E-5	3.3130136542958875E-5	0.12493581553703186	3.52007700768938E-5
80-81	0.001859428913473567	6.232606937144139E-4	2.4847602407219158E-5	3.3130136542958875E-5	0.12624652656401264	3.7271403610828735E-5
82-83	0.0018718527146771764	6.460376625876981E-4	2.6918235941154088E-5	3.3130136542958875E-5	0.1276773343359617	4.348330421263352E-5
84-85	0.0018967003170843955	6.563908302573727E-4	2.8988869475089015E-5	3.3130136542958875E-5	0.12893420889106022	4.9695204814438316E-5
86-87	0.00191533601888981	6.626027308591774E-4	2.8988869475089015E-5	3.3130136542958875E-5	0.13048925467504535	4.9695204814438316E-5
88-89	0.0019277598200934196	6.708852649949173E-4	2.8988869475089015E-5	3.3130136542958875E-5	0.13202152349015717	5.5907105416243096E-5
90-91	0.001942254254830964	6.915916003342665E-4	3.3130136542958875E-5	3.3130136542958875E-5	0.13283735310252753	5.797773895017803E-5
92-93	0.0019505367889667037	7.019447680039412E-4	3.3130136542958875E-5	3.3130136542958875E-5	0.13358485180827806	6.004837248411296E-5
94-95	0.001960889956636378	7.102273021396808E-4	3.3130136542958875E-5	3.3130136542958875E-5	0.1343468449487661	6.21190060180479E-5
96-97	0.0019629605901703134	7.226511033432905E-4	3.3130136542958875E-5	3.52007700768938E-5	0.1350591428844397	6.626027308591775E-5
98-99	0.001975384391373923	7.412868051487048E-4	3.7271403610828735E-5	3.7271403610828735E-5	0.13577351145364727	6.833090661985267E-5
100-101	0.0019878081925775327	7.433574386826397E-4	4.348330421263352E-5	4.1412670678698595E-5	0.13643818481804038	7.454280722165747E-5
102-103	0.0019919494596454023	7.454280722165746E-4	4.762457128050339E-5	4.1412670678698595E-5	0.13711114071656924	7.868407428952734E-5
104-105	0.0020064438943829467	7.474987057505096E-4	4.9695204814438316E-5	4.5553937746568455E-5	0.137755107745623	8.696660842526704E-5
106-107	0.0020416446644598405	7.516399728183795E-4	4.9695204814438316E-5	5.176583834837324E-5	0.13835559147046414	9.110787549313691E-5
108-109	0.00205820973273132	7.619931404880542E-4	4.9695204814438316E-5	5.383647188230817E-5	0.1390347592695948	9.73197760949417E-5
110-111	0.0020789160680706694	7.785582087595335E-4	4.9695204814438316E-5	5.797773895017803E-5	0.13970771516812364	1.1595547790035606E-4
112-113	0.0020892692357403443	7.847701093613383E-4	4.9695204814438316E-5	5.797773895017803E-5	0.14035996473131312	1.263086455700307E-4
114-115	0.0020954811363421487	7.909820099631431E-4	4.9695204814438316E-5	5.797773895017803E-5	0.14095837782262033	1.3044991263790057E-4
116-117	0.0021016930369439535	7.95123277031013E-4	4.9695204814438316E-5	5.797773895017803E-5	0.1416437575223528	1.4287371384151017E-4
118-119	0.0021120462046136284	7.992645440988829E-4	5.383647188230817E-5	6.21190060180479E-5	0.1422960070855423	1.6565068271479435E-4
120-121	0.0021120462046136284	8.034058111667527E-4	5.383647188230817E-5	6.21190060180479E-5	0.14294825664873181	1.739332168505341E-4
122-123	0.0021120462046136284	8.054764447006876E-4	5.383647188230817E-5	6.21190060180479E-5	0.1435342459388354	1.7807448391840395E-4
124-125	0.002116187471681498	8.116883453024924E-4	6.21190060180479E-5	6.21190060180479E-5	0.14418442486849098	1.9256891865594846E-4
126-127	0.0021327525399529776	8.199708794382323E-4	6.21190060180479E-5	6.21190060180479E-5	0.14501267828206493	2.0706335339349297E-4
128-129	0.002147246974690522	8.241121465061021E-4	6.21190060180479E-5	6.21190060180479E-5	0.1457870952237566	2.174165210631676E-4
130-131	0.0021555295088262617	8.365359477097115E-4	6.418963955198283E-5	6.626027308591775E-5	0.1465180288612356	2.2776968873284228E-4
132-133	0.002182447744767416	8.489597489133212E-4	7.04015401537876E-5	6.833090661985267E-5	0.14717234905795906	2.298403222667772E-4
134-135	0.0022052247136407	8.55171649515126E-4	7.04015401537876E-5	7.04015401537876E-5	0.14773556137918936	2.4226412347038678E-4
136-137	0.0022114366142425047	8.655248171848006E-4	7.247217368772254E-5	7.04015401537876E-5	0.1484002347435825	2.7125299294547577E-4
138-139	0.0022114366142425047	8.738073513205403E-4	7.454280722165747E-5	7.247217368772254E-5	0.1489634470648128	2.981712288866299E-4
>>END_MODULE
