##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.2d.R.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24057984
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	57-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.84011361883024	36.0	35.0	38.0	33.0	39.0
2	35.226894073917414	35.0	34.0	37.0	32.0	39.0
3	35.34430017078738	35.0	34.0	37.0	33.0	39.0
4	35.409113747851855	36.0	34.0	37.0	33.0	39.0
5	35.3234236916942	35.0	34.0	37.0	33.0	39.0
6	35.28308623033418	35.0	34.0	37.0	33.0	39.0
7	35.32252008314579	35.0	34.0	37.0	33.0	39.0
8	35.46255184973105	36.0	34.0	38.0	33.0	39.0
9	35.436492808374965	36.0	34.0	38.0	33.0	39.0
10-14	35.395822800447455	35.4	34.2	37.6	33.0	39.0
15-19	35.367395838321286	35.8	34.0	37.8	33.0	39.0
20-24	35.33919882064931	35.6	34.0	37.6	32.8	39.0
25-29	35.32701763373024	35.8	34.0	37.6	32.6	39.0
30-34	35.31089146122967	35.6	34.0	37.4	32.0	39.0
35-39	35.28454364255957	35.4	34.0	37.0	32.0	39.0
40-44	35.26687448125329	35.6	34.0	37.0	32.0	39.0
45-49	35.236524930767274	35.0	34.0	37.0	32.0	39.0
50-54	35.21649210507414	35.0	34.0	37.0	32.0	39.0
55-59	35.185304124949354	35.0	34.0	37.0	32.0	39.0
60-64	35.1480212120403	35.0	34.0	37.0	32.0	39.0
65-69	35.11218893975638	35.0	34.0	37.0	32.0	39.0
70-74	35.09371904279102	35.0	34.0	37.0	32.0	39.0
75-79	35.04289513875773	35.0	34.0	37.0	32.0	39.0
80-84	34.97553510064936	35.0	34.0	37.0	32.0	39.0
85-89	34.95653490368781	35.0	34.0	37.0	31.4	39.0
90-94	34.90682004726345	35.0	34.0	37.0	31.0	39.0
95-99	34.846604981764244	35.0	34.0	37.0	31.0	39.0
100-104	34.78551140773364	35.0	34.0	37.0	31.0	39.0
105-109	34.730381908620714	35.0	34.0	37.0	31.0	39.0
110-114	34.66702911915781	35.0	34.0	37.0	30.8	39.0
115-119	34.61041587914738	35.0	34.0	37.0	30.0	39.0
120-124	34.538702906869354	35.0	34.0	37.0	30.0	39.0
125-129	34.46086783810853	35.0	34.0	37.0	30.0	39.0
130-134	34.38939646140419	35.0	34.0	37.0	30.0	39.0
135-139	34.30449419973698	35.0	34.0	37.0	29.6	39.0
140-144	34.21242195614623	35.0	34.0	37.0	29.0	39.0
145-149	34.1769830546178	35.0	34.0	37.0	29.0	39.0
150	34.97242973111201	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	13.0
21	165.0
22	1674.0
23	8795.0
24	31025.0
25	77779.0
26	155213.0
27	264279.0
28	389742.0
29	518664.0
30	672916.0
31	883119.0
32	1185291.0
33	1649072.0
34	2458591.0
35	4141358.0
36	1.0065667E7
37	1554613.0
38	7.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.916428076434	18.35339985262273	16.81286761184977	31.917304459093497
2	24.212594039467312	24.854559717056922	33.534181417694846	17.398664825780912
3	19.7193372478758	28.886140251818276	30.50661269040664	20.887909809899284
4	23.43751662649705	33.73922353593717	22.365568952078448	20.457690885487327
5	25.153296302799106	35.02722006964507	22.40258784775981	17.41689577979601
6	18.727990674530336	38.68657905832841	22.88635656254489	19.699073704596362
7	20.101006800902354	20.27902254819024	38.19965130910387	21.420319341803538
8	21.39922447367161	22.981684583379888	28.38396184817481	27.23512909477369
9	23.357070291387274	23.075803985728978	28.651163316558996	24.915962406324756
10-14	23.025628606500266	28.974708179826774	25.653553727972835	22.346109485700126
15-19	22.91308116257788	28.103845276478694	27.08702857230265	21.896044988640778
20-24	22.9889608244699	28.197374497599696	26.960532945316874	21.853131732613534
25-29	23.102971554058726	28.16363083457035	26.896135603049697	21.83726200832123
30-34	23.084462106218044	27.949322769522166	27.05840855160599	21.9078065726538
35-39	23.181527595994744	27.93818883577277	27.00380547264476	21.876478095587725
40-44	23.34621471192266	27.725032155645295	27.087535680462672	21.841217451969374
45-49	23.339204980766468	27.540670074433503	27.177744402856035	21.942380541943997
50-54	23.423418188323677	27.525002926263483	27.124736636286734	21.92684224912611
55-59	23.599208871353625	27.412453635557387	27.021539743671617	21.96679774941737
60-64	23.52088838574887	27.37899757974709	27.075952207062958	22.024161827441084
65-69	23.596285466346554	27.301901494986968	27.052869735224107	22.04894330344237
70-74	23.661015440930168	27.26302237462014	26.958760434615165	22.11720174983453
75-79	23.592121527291738	27.31025185715809	27.04835154446257	22.049275071087607
80-84	23.68577096714923	27.364676519958568	26.926690699775698	22.0228618131165
85-89	23.718361710082647	27.273294167384343	26.995018193842	22.013325928691007
90-94	23.658009917798278	27.203189714419175	27.04019423217479	22.098606135607753
95-99	23.7391625032589	27.339224954375435	27.02442127966927	21.897191262696396
100-104	23.883151262823986	27.320523671837666	26.8284210516952	21.967904013643146
105-109	23.663267983071997	27.447916196851985	26.9354345448258	21.953381275250223
110-114	23.696726965547928	27.444954616482175	26.88102206161853	21.977296356351367
115-119	23.77982650501222	27.44891029727029	26.892994330429186	21.878268867288302
120-124	23.7534768367071	27.339499471536698	27.079865233606753	21.827158458149448
125-129	23.74345115026879	27.417309869588735	27.0356302390881	21.803608741054376
130-134	23.78326628612145	27.342913758652305	27.095459336325607	21.778360618900642
135-139	23.70370792942446	27.35664332500796	27.187980610960366	21.751668134607215
140-144	23.72900739847173	27.46094622417629	27.169567315882592	21.640479061469385
145-149	23.42165859414165	27.334009755613287	27.348902000987273	21.895429649257785
150	23.562190096883832	26.205607002042143	27.79438302302215	22.437819878051876
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	2.5
2	9.0
3	21.0
4	31.0
5	31.5
6	24.5
7	17.0
8	13.5
9	8.5
10	6.5
11	9.0
12	12.5
13	20.0
14	26.0
15	48.0
16	80.5
17	128.0
18	217.5
19	376.5
20	594.0
21	941.0
22	1470.0
23	2392.5
24	3934.0
25	6306.0
26	10014.5
27	16099.5
28	26763.5
29	41300.5
30	54780.0
31	70463.5
32	92443.0
33	121127.5
34	164512.5
35	229457.0
36	322838.0
37	442201.0
38	583510.5
39	762449.0
40	972140.0
41	1171972.5
42	1359233.5
43	1535755.0
44	1665657.5
45	1732712.0
46	1745061.0
47	1701679.0
48	1606110.0
49	1467507.0
50	1285402.5
51	1086347.0
52	894663.0
53	717494.0
54	566380.5
55	437226.5
56	329733.5
57	243854.5
58	176668.5
59	126510.0
60	87700.5
61	59672.5
62	40605.5
63	27695.5
64	18672.5
65	12673.0
66	8984.5
67	6693.5
68	5803.0
69	4423.0
70	2703.5
71	1977.0
72	1154.0
73	883.0
74	770.0
75	466.0
76	300.5
77	110.0
78	28.0
79	4.0
80	1.5
81	0.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	4.156624262448591E-6
10-14	8.313248524897182E-7
15-19	0.0
20-24	0.027000599884013557
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	8.313258545873911E-7
75-79	5.81097632241631E-4
80-84	0.05271877011428081
85-89	1.6626692771904454E-6
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	8.313910514155339E-7
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	2.56251709731375E-4
145-149	0.027463109980650317
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
56-57	1.0
58-59	0.0
60-61	2.0
62-63	0.0
64-65	3.0
66-67	5.0
68-69	7.0
70-71	9.0
72-73	16.0
74-75	12.0
76-77	18.0
78-79	24.0
80-81	43.0
82-83	47.0
84-85	50.0
86-87	86.0
88-89	88.0
90-91	100.0
92-93	87.0
94-95	102.0
96-97	134.0
98-99	141.0
100-101	156.0
102-103	147.0
104-105	165.0
106-107	159.0
108-109	143.0
110-111	157.0
112-113	202.0
114-115	213.0
116-117	246.0
118-119	265.0
120-121	306.0
122-123	329.0
124-125	332.0
126-127	368.0
128-129	472.0
130-131	561.0
132-133	724.0
134-135	934.0
136-137	1521.0
138-139	3230.0
140-141	6531.0
142-143	10878.0
144-145	70902.0
146-147	826825.0
148-149	1075963.0
150-151	2.205528E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.60887137755966
#Duplication Level	Percentage of total
1	28.224655023982635
2	10.597943098001805
3	7.107972647882417
4	5.0483451637111525
5	3.9091619769865575
6	3.154923653633105
7	2.6305173880844954
8	2.1478157431450584
9	1.8616891158565705
>10	21.386786155383938
>50	5.595672708845654
>100	6.453091136633267
>500	0.9813871598276193
>1k	0.7934611211989434
>5k	0.058795781133767405
>10k+	0.04778212569307247
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.156624262448591E-6	8.313248524897181E-6	4.156624262448591E-6	0.0	0.0011389150479109138	0.0
2	2.0783121312242953E-5	1.6626497049794362E-5	4.156624262448591E-6	0.0	0.004767648029028534	0.0
3	4.1566242624485906E-5	1.6626497049794362E-5	4.156624262448591E-6	0.0	0.006234936393672886	0.0
4	8.728910951142041E-5	1.6626497049794362E-5	4.156624262448591E-6	0.0	0.007515176666507052	0.0
5	8.728910951142041E-5	1.6626497049794362E-5	4.156624262448591E-6	0.0	0.008920115667214675	0.0
6	8.728910951142041E-5	1.6626497049794362E-5	4.156624262448591E-6	0.0	0.010674211105967982	0.0
7	9.975898229876617E-5	3.3252994099588725E-5	4.156624262448591E-6	4.156624262448591E-6	0.012715113618830239	0.0
8	1.6210834623549505E-4	3.3252994099588725E-5	4.156624262448591E-6	4.156624262448591E-6	0.018297460003298696	0.0
9	1.6210834623549505E-4	3.7409618362037316E-5	4.156624262448591E-6	4.156624262448591E-6	0.02424558932286263	0.0
10-11	1.8289146754773801E-4	4.1566242624485906E-5	4.156624262448591E-6	4.156624262448591E-6	0.030341278803743488	0.0
12-13	2.203010859097753E-4	5.1957803280607386E-5	4.156624262448591E-6	4.156624262448591E-6	0.035952721558049086	0.0
14-15	2.4731914361569114E-4	5.8192739674280275E-5	4.156624262448591E-6	4.156624262448591E-6	0.043752626986533864	0.0
16-17	2.701805770591584E-4	6.027105180550457E-5	4.156624262448591E-6	8.313248524897181E-6	0.04957605757822434	0.0
18-19	3.429215016520087E-4	6.442767606795315E-5	4.156624262448591E-6	8.313248524897181E-6	0.05664855376078062	0.0
20-21	3.990359291950647E-4	6.858430033040175E-5	8.313248524897181E-6	8.313248524897181E-6	0.06211867129016296	0.0
22-23	4.489154203444478E-4	9.1445733773869E-5	1.2469872787345772E-5	8.313248524897181E-6	0.06949252273174678	0.0
24-25	5.174997206748496E-4	9.1445733773869E-5	1.2469872787345772E-5	8.313248524897181E-6	0.07470908618111975	0.0
26-27	5.507527147744383E-4	9.768067016754188E-5	1.2469872787345772E-5	8.313248524897181E-6	0.07863086117273999	0.0
28-29	6.318068878921858E-4	1.1222885508611195E-4	1.2469872787345772E-5	8.313248524897181E-6	0.08262122046469064	0.0
30-31	8.063851069150267E-4	1.1430716721733625E-4	2.0783121312242953E-5	8.313248524897181E-6	0.08783570560193239	0.0
32-33	9.684934531505217E-4	1.2469872787345773E-4	2.0783121312242953E-5	8.313248524897181E-6	0.0940020576952749	0.0
34-35	0.0010952704931552037	1.2677704000468203E-4	2.0783121312242953E-5	8.313248524897181E-6	0.09705509821604338	0.0
36-37	0.0011576198570919325	1.2885535213590633E-4	2.0783121312242953E-5	8.313248524897181E-6	0.10001253637877555	0.0
38-39	0.0012386740302096802	1.3093366426713063E-4	2.0783121312242953E-5	8.313248524897181E-6	0.10336485384644034	0.0
40-41	0.0012843968970966146	1.413252249232521E-4	2.4939745574691544E-5	8.313248524897181E-6	0.1058858464616154	0.0
42-43	0.0013093366426713063	1.6003003410427076E-4	2.4939745574691544E-5	8.313248524897181E-6	0.10859180885646944	0.0
44-45	0.0013405113246396706	1.7873484328528942E-4	2.4939745574691544E-5	8.313248524897181E-6	0.11079066309130474	2.0783121312242953E-6
46-47	0.0013841558793953807	1.9536134033508378E-4	2.4939745574691544E-5	8.313248524897181E-6	0.11301030044745229	4.156624262448591E-6
48-49	0.0014319570584135396	1.9951796459753235E-4	2.4939745574691544E-5	8.313248524897181E-6	0.11516135350326943	4.156624262448591E-6
50-51	0.0014797582374316983	2.0367458885998095E-4	2.4939745574691544E-5	8.313248524897181E-6	0.1171336717158013	4.156624262448591E-6
52-53	0.0015275594164498572	2.1198783738487814E-4	2.4939745574691544E-5	8.313248524897181E-6	0.11930550789293068	4.156624262448591E-6
54-55	0.0016003003410427073	2.3900589509079397E-4	2.4939745574691544E-5	8.313248524897181E-6	0.12119053699595111	4.156624262448591E-6
56-57	0.0016564147685857633	2.4731914361569114E-4	2.4939745574691544E-5	8.313248524897181E-6	0.1231129757173336	4.156624262448591E-6
58-59	0.0017083725718663708	2.577107042718126E-4	2.4939745574691544E-5	8.313248524897181E-6	0.12465300500657078	4.156624262448591E-6
60-61	0.0017977399935090155	2.722588891903827E-4	2.4939745574691544E-5	1.2469872787345772E-5	0.12602053438891636	4.156624262448591E-6
62-63	0.0018330712997398286	2.868070741089528E-4	2.4939745574691544E-5	1.2469872787345772E-5	0.12786607556144355	4.156624262448591E-6
64-65	0.00185801104531452	3.075901954211957E-4	2.4939745574691544E-5	1.2469872787345772E-5	0.12936038198379382	4.156624262448591E-6
66-67	0.0019349085941698192	3.30451628864663E-4	2.4939745574691544E-5	1.2469872787345772E-5	0.130418242858587	4.156624262448591E-6
68-69	0.001961926651875735	3.3668656525833585E-4	2.9096369837140138E-5	1.2469872787345772E-5	0.1315405314094481	4.156624262448591E-6
70-71	0.001984788085319202	3.4915643804568164E-4	3.3252994099588725E-5	1.2469872787345772E-5	0.13265450671178433	4.156624262448591E-6
72-73	0.0020450591371247067	3.678612472267003E-4	3.3252994099588725E-5	1.2469872787345772E-5	0.13338607258197527	6.234936393672886E-6
74-75	0.0020699988826993984	3.761744957515975E-4	3.7409618362037316E-5	1.2469872787345772E-5	0.13434833109873212	8.313248524897181E-6
76-77	0.0021011735646677627	3.9487930493261616E-4	4.1566242624485906E-5	1.2469872787345772E-5	0.13552881238926753	8.313248524897181E-6
78-79	0.002144818119423473	4.156624262448591E-4	4.1566242624485906E-5	1.2469872787345772E-5	0.13713534766670393	8.313248524897181E-6
80-81	0.002161444616473267	4.36445547557102E-4	4.1566242624485906E-5	1.2469872787345772E-5	0.1384509192457689	1.4548184918570067E-5
82-83	0.0021843060499167344	4.57228668869345E-4	4.1566242624485906E-5	1.2469872787345772E-5	0.13999510515926855	1.6626497049794362E-5
84-85	0.0022071674833602016	4.5930698100056933E-4	4.1566242624485906E-5	1.6626497049794362E-5	0.14144784533899432	1.6626497049794362E-5
86-87	0.0022341855410661178	4.6969854165669075E-4	4.3644554755710205E-5	1.6626497049794362E-5	0.14317492272004173	1.8704809181018658E-5
88-89	0.0022549686623783605	4.759334780503636E-4	4.57228668869345E-5	1.6626497049794362E-5	0.14487290373125197	2.0783121312242953E-5
90-91	0.002279908407953052	4.987949114938309E-4	4.57228668869345E-5	1.6626497049794362E-5	0.14584139718440248	2.0783121312242953E-5
92-93	0.0022902999686091738	5.029515357562795E-4	4.780117901815879E-5	1.6626497049794362E-5	0.14677871595558464	2.0783121312242953E-5
94-95	0.0023110830899214165	5.029515357562795E-4	4.987949114938309E-5	1.6626497049794362E-5	0.14777007084217864	2.286143344346725E-5
96-97	0.002315239714183865	5.091864721499524E-4	5.1957803280607386E-5	2.0783121312242953E-5	0.14860763063106203	2.4939745574691544E-5
98-99	0.0023256312748399866	5.195780328060739E-4	5.611442754305598E-5	2.0783121312242953E-5	0.14944726873207664	2.4939745574691544E-5
100-101	0.0023339445233648837	5.195780328060739E-4	5.8192739674280275E-5	2.0783121312242953E-5	0.1502141659084984	2.4939745574691544E-5
102-103	0.002336022835496108	5.195780328060739E-4	6.027105180550457E-5	2.0783121312242953E-5	0.1511036835006624	2.4939745574691544E-5
104-105	0.002348492708283454	5.195780328060739E-4	6.234936393672887E-5	2.7018057705915842E-5	0.1519661830351205	2.4939745574691544E-5
106-107	0.002371354141726921	5.362045298558683E-4	6.650598819917745E-5	3.3252994099588725E-5	0.1527621765813794	3.3252994099588725E-5
108-109	0.002385902326645491	5.632225875617841E-4	6.650598819917745E-5	3.3252994099588725E-5	0.15354362194271973	4.1566242624485906E-5
110-111	0.00240460713582651	5.736141482179056E-4	6.650598819917745E-5	3.3252994099588725E-5	0.15431259743127274	4.1566242624485906E-5
112-113	0.0024066854479577343	5.777707724803542E-4	6.650598819917745E-5	3.3252994099588725E-5	0.15499428381031427	4.1566242624485906E-5
114-115	0.0024066854479577343	5.819273967428028E-4	6.650598819917745E-5	3.3252994099588725E-5	0.155686361750012	4.1566242624485906E-5
116-117	0.0024149986964826313	5.819273967428028E-4	6.858430033040175E-5	3.3252994099588725E-5	0.15651976491463293	4.57228668869345E-5
118-119	0.00241915532074508	5.923189573989241E-4	7.481923672407463E-5	3.3252994099588725E-5	0.15728250546679223	4.780117901815879E-5
120-121	0.00241915532074508	5.943972695301485E-4	7.481923672407463E-5	3.3252994099588725E-5	0.15798913159140848	5.403611541183168E-5
122-123	0.0024212336328763044	5.98553893792597E-4	7.481923672407463E-5	3.3252994099588725E-5	0.15872069746159945	5.819273967428027E-5
124-125	0.002431625193532426	6.068671423174942E-4	7.481923672407463E-5	3.3252994099588725E-5	0.1594959078865461	6.650598819917745E-5
126-127	0.002439938442057323	6.151803908423914E-4	7.481923672407463E-5	3.3252994099588725E-5	0.16044361821838438	6.650598819917745E-5
128-129	0.0024440950663197716	6.214153272360644E-4	7.481923672407463E-5	3.3252994099588725E-5	0.1613767803653041	6.650598819917745E-5
130-131	0.0024503300027134446	6.40120136417083E-4	7.481923672407463E-5	3.3252994099588725E-5	0.16216653897516933	6.650598819917745E-5
132-133	0.0024877396210754815	6.421984485483073E-4	7.481923672407463E-5	3.3252994099588725E-5	0.16298955057913417	6.858430033040175E-5
134-135	0.0025106010545189487	6.463550728107558E-4	7.897586098652323E-5	3.5331306230813024E-5	0.1637980139981804	7.274092459285035E-5
136-137	0.0025168359909126217	6.505116970732045E-4	7.897586098652323E-5	3.7409618362037316E-5	0.16471870627231275	7.481923672407463E-5
138-139	0.0025230709273062948	6.54668321335653E-4	7.897586098652323E-5	3.7409618362037316E-5	0.16550015163365311	7.897586098652323E-5
>>END_MODULE
