##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.2d.R.3.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24221274
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	73-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.632201179838844	36.0	34.0	38.0	32.0	39.0
2	34.90651503302428	35.0	34.0	38.0	32.0	39.0
3	35.06333737853756	35.0	34.0	38.0	32.0	39.0
4	35.0589434725853	36.0	34.0	38.0	31.0	39.0
5	34.91563094492883	35.0	34.0	37.0	31.0	39.0
6	34.88148348431218	35.0	34.0	37.0	32.0	39.0
7	34.872401674660054	35.0	34.0	37.0	32.0	39.0
8	35.034007707439336	35.0	34.0	37.0	32.0	39.0
9	35.005539758148146	35.0	34.0	37.0	32.0	39.0
10-14	34.99220052586829	35.2	34.0	37.4	32.0	39.0
15-19	35.001917950310954	35.0	34.0	37.0	32.0	39.0
20-24	34.99700041376849	35.0	34.0	37.0	32.0	39.0
25-29	34.98877395136193	35.0	34.0	37.0	32.0	39.0
30-34	34.97565663969616	35.0	34.0	37.0	32.0	39.0
35-39	34.96590371753361	35.0	34.0	37.0	32.0	39.0
40-44	34.93046040435363	35.0	34.0	37.0	31.6	39.0
45-49	34.85892681780488	35.0	34.0	37.0	31.0	39.0
50-54	34.832607037928724	35.0	34.0	37.0	31.0	39.0
55-59	34.81885577117042	35.0	34.0	37.0	31.0	39.0
60-64	34.796631168121046	35.0	34.0	37.0	31.0	39.0
65-69	34.76513919127458	35.0	34.0	37.0	31.0	39.0
70-74	34.757763598607134	35.0	34.0	37.0	31.0	39.0
75-79	34.73856917537417	35.0	34.0	37.0	31.0	39.0
80-84	34.72454870163756	35.0	34.0	37.0	31.0	39.0
85-89	34.702644435867704	35.0	34.0	37.0	31.0	39.0
90-94	34.68091521285382	35.0	34.0	37.0	31.0	39.0
95-99	34.65882930209366	35.0	34.0	37.0	31.0	39.0
100-104	34.62224576813826	35.0	34.0	37.0	31.0	39.0
105-109	34.592085479494735	35.0	34.0	37.0	30.2	39.0
110-114	34.5597479840838	35.0	34.0	37.0	30.0	39.0
115-119	34.52603839047954	35.0	34.0	37.0	30.0	39.0
120-124	34.48476763875552	35.0	34.0	37.0	30.0	39.0
125-129	34.46692689794378	35.0	34.0	37.0	30.0	39.0
130-134	34.41815776287535	35.0	34.0	37.0	30.0	39.0
135-139	34.38341357218785	35.0	34.0	37.0	30.0	39.0
140-144	34.338227003238494	35.0	34.0	37.0	30.0	39.0
145-149	34.36530881406232	35.0	34.0	37.0	30.0	39.0
150	35.15922436941697	35.0	34.0	37.0	32.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	10.0
22	156.0
23	2087.0
24	14792.0
25	63366.0
26	170552.0
27	328206.0
28	499600.0
29	659291.0
30	827322.0
31	1045568.0
32	1350105.0
33	1802965.0
34	2564676.0
35	4197324.0
36	9383379.0
37	1311867.0
38	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.050670745064856	10.885942663461881	15.031207689570747	39.03217890190252
2	22.349187360041373	12.82063657412616	36.90656499400087	27.9236110718316
3	20.982771401483546	17.576087198182563	28.42508411476652	33.01605728556737
4	26.1971285544718	25.360883521700984	22.346922640243587	26.09506528358363
5	25.77716048699712	30.888869823905058	24.509509252453178	18.824460436644646
6	19.154670452212795	36.321342926451536	25.992215431308008	18.531771190027666
7	16.040434243448686	24.70579968368274	40.41183750534448	18.841928567524093
8	18.46534402274236	24.269619139537614	31.717856807702567	25.547180030017465
9	19.64323188800169	22.994809139076093	32.77452587757439	24.587433095347826
10-14	20.341868222816462	29.198350150532082	27.066283765986675	23.39349786066478
15-19	20.502952218153712	27.890882451899163	27.855664560196193	23.75050076975093
20-24	20.679222476864155	28.048146151366698	27.557598217838976	23.71503315393017
25-29	20.673735488795206	28.22569173604426	27.445707988750517	23.65486478641002
30-34	20.65417328466467	27.93015142893227	27.46684154379323	23.948833742609832
35-39	20.72385435528463	27.943410447360844	27.5067417871553	23.82599341019923
40-44	20.79450244857127	28.01040862224204	27.397900423441808	23.797188505744888
45-49	20.88119609037908	27.781431046211143	27.3611295728741	23.976243290535677
50-54	21.004791322817347	27.76235499908795	27.448570127541178	23.784283550553525
55-59	21.002187592576295	27.80737242090906	27.368359349496167	23.822080637018477
60-64	20.947167629016345	27.723544792967513	27.33778745754748	23.991500120468658
65-69	21.047180184835668	27.658324765495156	27.414356249740777	23.880138799928396
70-74	21.17374089848414	27.580970255858084	27.393918725878606	23.85137011977917
75-79	21.192522460266837	27.472487638266767	27.396982831522653	23.938007069943744
80-84	21.220458798333635	27.486806331309527	27.381772892639994	23.910961977716845
85-89	21.208434987659952	27.51215415726441	27.41639101510772	23.86301983996792
90-94	21.214849003700888	27.387325446188814	27.35862988747967	24.03919566263063
95-99	21.289709220787067	27.281353105813587	27.505493100308637	23.923444573090705
100-104	21.34150051699218	27.357709731529283	27.342371015547435	23.958418735931097
105-109	21.31857482663059	27.18726158313485	27.459830364342565	24.034333225891995
110-114	21.403938915311763	27.163146439771413	27.500592319240567	23.932322325676257
115-119	21.440250725763832	27.205564972042286	27.45242430028345	23.90176000191043
120-124	21.39206306379024	27.130592176768864	27.4495856928134	24.0277590666275
125-129	21.416754418159172	27.06091456176901	27.507274190118313	24.015056829953505
130-134	21.505738998131015	27.166317283017815	27.400047499804025	23.927896219047142
135-139	21.46027618634701	27.037032987536513	27.51348171245227	23.98920911366421
140-144	21.539597180057683	26.966558580547666	27.62162203823766	23.87222220115699
145-149	21.16284124999775	27.064140550266043	27.755243569972553	24.017774629763654
150	21.281051017690732	25.60073300318349	28.3044938267586	24.81372215236718
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.5
7	3.0
8	3.5
9	5.0
10	4.5
11	7.5
12	14.5
13	20.0
14	33.0
15	67.5
16	147.5
17	306.0
18	586.0
19	1087.5
20	2046.0
21	3536.0
22	5558.5
23	8923.5
24	14323.5
25	22582.0
26	33468.5
27	46475.5
28	63559.5
29	89635.0
30	122404.5
31	155933.0
32	186018.0
33	219480.5
34	258692.0
35	305534.0
36	378162.5
37	477870.0
38	595967.0
39	743502.5
40	910073.5
41	1071568.0
42	1241657.5
43	1417117.0
44	1559598.0
45	1648623.0
46	1675775.0
47	1640092.5
48	1557704.5
49	1437548.0
50	1279513.5
51	1103367.5
52	923601.5
53	744965.5
54	580653.0
55	442037.5
56	333828.5
57	251174.5
58	189164.5
59	140024.0
60	99043.5
61	70500.0
62	50480.0
63	33891.5
64	22729.5
65	16549.0
66	14315.5
67	10503.5
68	5778.0
69	4133.0
70	4151.0
71	3009.5
72	1319.5
73	419.0
74	181.5
75	103.5
76	65.0
77	43.5
78	16.5
79	2.0
80	3.0
81	2.5
82	0.0
83	0.0
84	0.5
85	0.5
86	0.0
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0011436227508098873
3	0.09408258211355852
4	9.908644772360033E-5
5	9.082924374663364E-5
6	9.082924374663364E-5
7	9.082924374663364E-5
8	1.6927268152781724E-4
9	2.1055870141265074E-4
10-14	2.4028463572973083E-4
15-19	2.5845048447905753E-4
20-24	3.02213665556981E-4
25-29	3.179023531132177E-4
30-34	3.179023531132177E-4
35-39	1.0651793130287036E-4
40-44	1.94870013856414E-4
45-49	1.6844696113012058E-4
50-54	1.2303233925680374E-4
55-59	1.4945539198309718E-4
60-64	1.511068327784905E-4
65-69	0.03804671876466944
70-74	1.395467483630031E-4
75-79	1.3294099720181495E-4
80-84	1.0073793343693156E-4
85-89	8.257216045095234E-5
90-94	1.4285003928210936E-4
95-99	1.321159705401864E-4
100-104	8.835277562308938E-5
105-109	1.3459393850995824E-4
110-114	7.101302549814337E-5
115-119	7.266489081930879E-5
120-124	1.1064969545859546E-4
125-129	1.081736461170182E-4
130-134	6.0280998636493356E-5
135-139	6.523851195842982E-5
140-144	4.378262011720525E-5
145-149	5.217266324111058E-5
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
72-73	1.0
74-75	0.0
76-77	2.0
78-79	4.0
80-81	4.0
82-83	5.0
84-85	13.0
86-87	13.0
88-89	11.0
90-91	14.0
92-93	23.0
94-95	27.0
96-97	27.0
98-99	21.0
100-101	25.0
102-103	26.0
104-105	37.0
106-107	40.0
108-109	34.0
110-111	37.0
112-113	40.0
114-115	54.0
116-117	80.0
118-119	76.0
120-121	75.0
122-123	92.0
124-125	99.0
126-127	152.0
128-129	130.0
130-131	186.0
132-133	262.0
134-135	387.0
136-137	712.0
138-139	2228.0
140-141	5165.0
142-143	8177.0
144-145	71196.0
146-147	762576.0
148-149	1251993.0
150-151	2.211723E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.63887622527523
#Duplication Level	Percentage of total
1	38.22171262344932
2	10.380538009460814
3	6.392001981753498
4	4.436606990478017
5	3.424563691993561
6	2.7791896129981497
7	2.276055537705321
8	1.8448761793094983
9	1.6249626332716847
>10	18.324112953653092
>50	4.615894019051928
>100	4.782682354670961
>500	0.49257011575403253
>1k	0.3806781064827757
>5k	0.023555189967325932
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	4.128601988483347E-6	8.257203976966694E-6	0.0	1.2385805965450042E-5	1.7752988550478395E-4	2.8900213919383432E-5
2	8.257203976966694E-6	4.954322386180017E-5	0.0	1.651440795393339E-5	0.0020354007803222904	6.19290298272502E-5
3	1.651440795393339E-5	5.7800427838766865E-5	0.0	2.0643009942416738E-5	0.0031212231032934105	1.0321504971208369E-4
4	6.19290298272502E-5	7.018623380421691E-5	0.0	2.0643009942416738E-5	0.004553847993297132	1.1972945766601707E-4
5	6.605763181573356E-5	7.018623380421691E-5	0.0	2.4771611930900083E-5	0.005866743425634836	1.4037246760843382E-4
6	8.670064175815029E-5	7.018623380421691E-5	0.0	2.4771611930900083E-5	0.006886508116790224	1.486296715854005E-4
7	1.1147225368905038E-4	1.0734365170056702E-4	0.0	2.4771611930900083E-5	0.008059031081519494	1.486296715854005E-4
8	1.321152636314671E-4	1.1147225368905038E-4	0.0	2.4771611930900083E-5	0.010053145841956952	1.651440795393339E-4
9	1.6927268152781724E-4	1.1560085567753373E-4	0.0	2.4771611930900083E-5	0.013430342268536328	1.651440795393339E-4
10-11	2.126230024068924E-4	1.3005096263722546E-4	0.0	2.8900213919383432E-5	0.01813281993341886	2.3120171135506743E-4
12-13	2.662948282571759E-4	1.321152636314671E-4	0.0	3.302881590786678E-5	0.022009577200604723	2.7661633322838427E-4
14-15	2.745520322341426E-4	1.424367686026755E-4	0.0	3.302881590786678E-5	0.026350802191494965	3.1170945013049274E-4
16-17	3.096451491362511E-4	1.651440795393339E-4	0.0	3.302881590786678E-5	0.030448439665064685	3.302881590786678E-4
18-19	3.3028815907866775E-4	1.878513904759923E-4	0.0	3.302881590786678E-5	0.03418069586265363	3.344167610671511E-4
20-21	4.087315968598514E-4	2.4565181831475916E-4	0.0	3.302881590786678E-5	0.037675557445904784	3.385453630556345E-4
22-23	5.140109475661767E-4	2.642305272629342E-4	0.0	3.302881590786678E-5	0.04162869384987759	3.468025670326012E-4
24-25	5.821328803761519E-4	2.84873537205351E-4	0.0	3.302881590786678E-5	0.04609377690042234	3.612526739922929E-4
26-27	6.358047062264354E-4	2.890021391938343E-4	0.0	3.302881590786678E-5	0.050319401035635036	3.942814899001597E-4
28-29	6.936051340652024E-4	2.890021391938343E-4	0.0	3.302881590786678E-5	0.05450993205394564	4.149244998425764E-4
30-31	7.534698628982109E-4	2.890021391938343E-4	0.0	3.302881590786678E-5	0.058708720276233195	4.541462187331682E-4
32-33	7.864986788060776E-4	2.890021391938343E-4	0.0	3.92217188905918E-5	0.06254625582452847	5.346539575085934E-4
34-35	8.463634076390863E-4	2.890021391938343E-4	0.0	4.541462187331682E-5	0.06591313074613664	5.429111614855601E-4
36-37	8.773279225527113E-4	2.890021391938343E-4	0.0	4.954322386180017E-5	0.06913963320013639	5.635541714279769E-4
38-39	9.000352334893698E-4	2.890021391938343E-4	0.0	5.367182585028351E-5	0.07327855669359093	5.75939977393427E-4
40-41	9.309997484029948E-4	2.890021391938343E-4	0.0	5.367182585028351E-5	0.07664130301321062	5.800685793819103E-4
42-43	9.433855543684448E-4	2.993236441650427E-4	0.0	5.367182585028351E-5	0.07989876998212397	5.88325783358877E-4
44-45	9.764143702763117E-4	3.0964514913625103E-4	0.0	6.19290298272502E-5	0.08390351391095283	5.903900843531187E-4
46-47	0.0010135717881726617	3.0964514913625103E-4	0.0	6.19290298272502E-5	0.08776375677018475	5.945186863416021E-4
48-49	0.0010527935070632536	3.240952560959428E-4	0.0	6.19290298272502E-5	0.09158477791052609	6.234189002609855E-4
50-51	0.0010775651189941535	3.4680256703260116E-4	0.0	6.19290298272502E-5	0.0956720938791246	6.399333082149188E-4
52-53	0.0011291726438501955	3.4886686802684287E-4	0.0	6.19290298272502E-5	0.09963555178806863	6.440619102034021E-4
54-55	0.0011972945766601708	3.529954700153262E-4	0.0	6.19290298272502E-5	0.10262053102574209	6.440619102034021E-4
56-57	0.0012220661885910707	3.6331697498653457E-4	0.0	6.19290298272502E-5	0.10666656097445576	6.605763181573355E-4
58-59	0.0012365162955507625	3.757027809519846E-4	0.0	6.399333082149187E-5	0.1106857550102443	6.667692211400606E-4
60-61	0.0012695451114586292	3.8808858691743466E-4	0.0	6.605763181573356E-5	0.11411662326267397	6.688335221343022E-4
62-63	0.0012943167233895295	3.9634579089440134E-4	0.0	6.812193280997524E-5	0.11734725431866218	6.688335221343022E-4
64-65	0.0013252812383031545	3.9634579089440134E-4	0.0	7.225053479845858E-5	0.12157700705586337	6.729621241227857E-4
66-67	0.0013521171512282963	4.1286019884833474E-4	0.0	7.431483579270025E-5	0.125323713360412	6.77090726111269E-4
68-69	0.0014305605890094799	4.396961117734765E-4	0.0	7.431483579270025E-5	0.12818277023743674	6.915408330709608E-4
70-71	0.0015379042407100468	4.624034227101349E-4	0.0	7.431483579270025E-5	0.13074869637327913	7.121838430133774E-4
72-73	0.0015957046685488138	4.768535296698266E-4	0.0	7.431483579270025E-5	0.13354375991948236	7.245696489788275E-4
74-75	0.001630797785450922	4.87175034641035E-4	0.0	7.84434377811836E-5	0.13644203851539766	7.348911539500358E-4
76-77	0.001651440795393339	4.954322386180016E-4	0.0	8.257203976966694E-5	0.1386879979971326	7.431483579270025E-4
78-79	0.0017092412232321058	4.974965396122434E-4	0.0	1.0734365170056702E-4	0.14093602177986178	7.452126589212443E-4
80-81	0.001758784447093906	4.99560840606485E-4	0.0	1.0734365170056702E-4	0.14353497673161206	7.493412609097276E-4
82-83	0.0018021347679729812	5.0575374358921E-4	0.0	1.0734365170056702E-4	0.14604723104160416	7.555341638924525E-4
84-85	0.0018681923997887146	5.181395495546602E-4	0.0	1.0940795269480871E-4	0.1481693324636846	7.555341638924525E-4
86-87	0.001930121429615965	5.243324525373851E-4	0.0	1.1147225368905038E-4	0.15019854034102417	7.555341638924525E-4
88-89	0.0019858575564604903	5.243324525373851E-4	0.0	1.2385805965450043E-4	0.15218852649947315	7.617270668751776E-4
90-91	0.00201062916839139	5.346539575085934E-4	4.128601988483347E-6	1.2798666164298377E-4	0.15476890274227523	7.720485718463859E-4
92-93	0.002076686800207124	5.387825594970768E-4	4.128601988483347E-6	1.2798666164298377E-4	0.15738643640297367	7.720485718463859E-4
94-95	0.0021406801310286157	5.511683654625269E-4	4.128601988483347E-6	1.2798666164298377E-4	0.1602062715611078	7.720485718463859E-4
96-97	0.002252152384717666	5.635541714279769E-4	4.128601988483347E-6	1.2798666164298377E-4	0.16245223104284273	7.761771738348693E-4
98-99	0.002320274317527641	5.69747074410702E-4	4.128601988483347E-6	1.321152636314671E-4	0.16443395999731475	7.761771738348693E-4
100-101	0.0023801390463606496	5.841971813703936E-4	4.128601988483347E-6	1.3624386561995045E-4	0.16647348937962553	7.761771738348693E-4
102-103	0.0024007820563030666	5.88325783358877E-4	6.192902982725021E-6	1.3624386561995045E-4	0.1682694312446158	7.761771738348693E-4
104-105	0.0024420680761879	5.924543853473603E-4	8.257203976966694E-6	1.3830816661419213E-4	0.16994151504995153	7.864986788060776E-4
106-107	0.0025081257080036335	5.965829873358437E-4	8.257203976966694E-6	1.424367686026755E-4	0.1714938693976213	7.885629798003194E-4
108-109	0.002574183339819367	5.986472883300854E-4	8.257203976966694E-6	1.5275827357388386E-4	0.1730441594442968	7.926915817888027E-4
110-111	0.0026484981756120674	6.110330942955353E-4	8.257203976966694E-6	1.5275827357388386E-4	0.17461302819992044	7.926915817888027E-4
112-113	0.002726941613393251	6.172259972782604E-4	8.257203976966694E-6	1.5482257456812554E-4	0.17616951114957868	7.926915817888027E-4
114-115	0.0027826777402377764	6.296118032437105E-4	8.257203976966694E-6	1.5895117655660885E-4	0.17803563924837315	7.926915817888027E-4
116-117	0.002832220964099576	6.358047062264355E-4	8.257203976966694E-6	1.6927268152781724E-4	0.1795487718771523	7.988844847715277E-4
118-119	0.002920985906851968	6.523191141803689E-4	8.257203976966694E-6	1.6927268152781724E-4	0.18106809740891416	8.009487857657694E-4
120-121	0.0030076865486101186	6.564477161688522E-4	8.257203976966694E-6	1.713369825220589E-4	0.18251517240587756	8.009487857657694E-4
122-123	0.003168702026160969	6.585120171630938E-4	8.257203976966694E-6	1.8165848749326729E-4	0.18392715428593887	8.009487857657694E-4
124-125	0.0032863671828327447	6.605763181573356E-4	8.257203976966694E-6	1.8372278848750894E-4	0.18529372154412688	8.009487857657694E-4
126-127	0.0033379747076887865	6.750264251170273E-4	8.257203976966694E-6	1.8578708948175062E-4	0.18657565246155094	8.050773877542527E-4
128-129	0.0034184824464642116	6.77090726111269E-4	8.257203976966694E-6	1.9404429345871733E-4	0.18781836166008442	8.050773877542527E-4
130-131	0.0035010544862338787	6.874122310824774E-4	1.0321504971208367E-5	2.02301497435684E-4	0.1890197848387331	8.09205989742736E-4
132-133	0.003587755127992029	6.936051340652023E-4	1.2385805965450042E-5	2.105587014126507E-4	0.1901840505994854	8.09205989742736E-4
134-135	0.0037570278095198457	6.956694350594441E-4	1.2385805965450042E-5	2.105587014126507E-4	0.19120794389262927	8.09205989742736E-4
136-137	0.003951072102978563	7.08055241024894E-4	1.2385805965450042E-5	2.105587014126507E-4	0.19229376621560038	8.09205989742736E-4
138-139	0.003967586510932497	7.348911539500358E-4	1.2385805965450042E-5	2.126230024068924E-4	0.19338578144155422	8.195274947139444E-4
>>END_MODULE
