##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.2d.R.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24221274
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	60-150
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.09719030468835	37.0	35.0	38.0	33.0	39.0
2	35.38055281485194	35.0	34.0	37.0	33.0	39.0
3	35.49154309554485	35.0	34.0	37.0	33.0	39.0
4	35.568693290039164	36.0	35.0	38.0	33.0	39.0
5	35.49034753498103	36.0	35.0	38.0	33.0	39.0
6	35.41393016733967	35.0	35.0	37.0	33.0	39.0
7	35.44925494009935	35.0	34.0	38.0	33.0	39.0
8	35.61821682046948	36.0	35.0	38.0	33.0	39.0
9	35.59365857468934	36.0	35.0	38.0	33.0	39.0
10-14	35.552418415315394	35.8	35.0	37.8	33.0	39.0
15-19	35.521690238094	36.0	35.0	38.0	33.0	39.0
20-24	35.49690493571891	36.0	35.0	38.0	33.0	39.0
25-29	35.483449863124456	36.0	34.8	38.0	33.0	39.0
30-34	35.466695913683154	36.0	34.2	38.0	33.0	39.0
35-39	35.44119804763366	36.0	34.0	38.0	33.0	39.0
40-44	35.424554051120516	36.0	34.0	38.0	33.0	39.0
45-49	35.39341202283579	36.0	34.0	38.0	32.8	39.0
50-54	35.37330112363206	36.0	34.0	38.0	32.0	39.0
55-59	35.34215081337175	36.0	34.0	38.0	32.0	39.0
60-64	35.309950174658105	36.0	34.0	38.0	32.0	39.0
65-69	35.27459750142408	36.0	34.0	38.0	32.0	39.0
70-74	35.25288520697862	36.0	34.0	38.0	32.0	39.0
75-79	35.20558644081662	36.0	34.0	38.0	32.0	39.0
80-84	35.14515082425457	36.0	34.0	38.0	32.0	39.0
85-89	35.119516658023755	36.0	34.0	38.0	32.0	39.0
90-94	35.0742455100184	36.0	34.0	38.0	32.0	39.0
95-99	35.013789451095235	35.2	34.0	38.0	31.2	39.0
100-104	34.957011685301254	35.2	34.0	38.0	31.0	39.0
105-109	34.89866091520966	35.0	34.0	37.6	31.0	39.0
110-114	34.83853893686917	35.0	34.0	37.6	31.0	39.0
115-119	34.78275425477183	35.0	34.0	37.0	31.0	39.0
120-124	34.71114231988517	35.0	34.0	37.0	30.2	39.0
125-129	34.640172338980804	35.0	34.0	37.0	30.0	39.0
130-134	34.56863891727737	35.0	34.0	37.0	30.0	39.0
135-139	34.48636152250988	35.0	34.0	37.0	30.0	39.0
140-144	34.39163887451771	35.0	34.0	37.0	30.0	39.0
145-149	34.3662240049236	35.0	34.0	37.0	29.2	39.0
150	35.12516789092743	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	21.0
21	197.0
22	1736.0
23	8806.0
24	30467.0
25	75163.0
26	150135.0
27	259595.0
28	376329.0
29	489513.0
30	620767.0
31	798457.0
32	1053422.0
33	1461123.0
34	2193396.0
35	3783427.0
36	1.0849713E7
37	2068991.0
38	16.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.5254949842853	18.971466983941472	16.117897018959447	31.385141012813776
2	24.182676112853358	25.856506385936033	33.160313250257325	16.80050425095328
3	19.518118658828598	29.787867475509337	30.055954117029515	20.638059748632546
4	23.008830997081326	34.454884577912786	22.457592445384993	20.078691979620892
5	24.900259168861226	35.372561327698946	22.646352128298453	17.080827375141375
6	18.738778975870552	38.858323472167484	23.159813971800162	19.243083580161805
7	20.201332101688788	20.50273243265404	38.364773876056226	20.931161589600944
8	21.43637448632966	22.998406277060408	28.779084865643316	26.786134370966614
9	23.456334295215026	22.987618240064496	29.02048835251193	24.53555911220855
10-14	23.13986126411022	28.894791413531756	25.862471974017552	22.10287534834047
15-19	22.95147848872819	28.097714894727915	27.272331377322782	21.67847523922111
20-24	23.029236291583448	28.239031280513743	27.087940617130123	21.643791810772683
25-29	23.14434657731051	28.215167377240352	27.0052128554427	21.635273190006437
30-34	23.161655328287026	27.989124766930097	27.137287658774678	21.7119322460082
35-39	23.252941195413584	27.987372588246185	27.084680186517023	21.675006029823205
40-44	23.39765364943231	27.74530604789822	27.193719042194065	21.6633212604754
45-49	23.411188858191355	27.546474227573658	27.277635354771178	21.764701559463802
50-54	23.48769719524575	27.512084253075773	27.247874364459513	21.75234418721896
55-59	23.659822352862196	27.402225002698046	27.129288079561793	21.808664564877965
60-64	23.599085811881153	27.354528393284696	27.18039799146808	21.865987803366075
65-69	23.681303663637536	27.268359735875407	27.137480533532138	21.912856066954912
70-74	23.723281548747245	27.233919724518245	27.05144275675367	21.991355969980837
75-79	23.666636379030905	27.283973437908582	27.12782614276125	21.92156404029927
80-84	23.763300436246897	27.32763648917327	27.009230663853607	21.899832410726226
85-89	23.79927478611356	27.247115772217107	27.057410478732425	21.896198962936907
90-94	23.711379884352066	27.171988284409526	27.14081570879155	21.97581612244686
95-99	23.810604758045038	27.301739163910042	27.11675092666288	21.770905151382035
100-104	23.969886430969055	27.281863912159015	26.903187070614067	21.845062586257864
105-109	23.742503323103133	27.436552529142265	26.99961673907214	21.82132740868246
110-114	23.76131263728544	27.4248264544909	26.940246232129557	21.873614676094107
115-119	23.849660652777757	27.429323386524317	26.953709002141892	21.767306958556034
120-124	23.837972367350005	27.318332618515196	27.146006583550218	21.69768843058458
125-129	23.797599671839162	27.414222793305377	27.090014564784155	21.698162970071312
130-134	23.84208133214555	27.31981926307341	27.171608560975763	21.666490843805285
135-139	23.766735517531572	27.331993232828083	27.26234142109834	21.638929828542008
140-144	23.80544323619263	27.452677010584775	27.22942830528934	21.512451447933255
145-149	23.525869428090132	27.472207722821423	27.264804461796732	21.737118387291712
150	23.81458775643522	26.19507202888693	27.639925557288404	22.350414657389443
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	3.5
3	10.5
4	18.0
5	17.5
6	13.5
7	13.0
8	10.0
9	9.0
10	8.5
11	7.5
12	11.5
13	19.5
14	27.0
15	38.0
16	75.0
17	138.5
18	213.0
19	371.5
20	609.0
21	946.0
22	1488.0
23	2465.0
24	4021.5
25	6580.0
26	10440.5
27	16542.0
28	27998.0
29	43555.5
30	58185.0
31	75527.5
32	99523.0
33	130932.0
34	176937.5
35	246509.5
36	343949.5
37	466102.5
38	612622.0
39	795973.5
40	1008119.5
41	1208237.5
42	1392892.5
43	1561304.0
44	1679561.5
45	1737393.0
46	1741691.0
47	1693053.0
48	1593047.5
49	1449847.0
50	1267193.5
51	1068841.5
52	878610.5
53	704359.5
54	553967.0
55	424719.0
56	319113.0
57	236266.0
58	171385.5
59	122889.0
60	85895.0
61	58772.5
62	40414.0
63	28210.5
64	19591.0
65	13553.0
66	9787.5
67	7703.5
68	7390.0
69	5767.0
70	3247.5
71	2342.5
72	1399.5
73	1014.0
74	857.0
75	568.5
76	366.5
77	104.5
78	23.5
79	4.0
80	2.5
81	2.5
82	1.5
83	0.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.128601988483347E-6
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	1.6514407953933389E-6
20-24	0.019520030201549268
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	1.6514407953933389E-6
55-59	0.0
60-64	0.0
65-69	0.0
70-74	8.257212567834241E-7
75-79	4.252471670714951E-4
80-84	0.03791567811803874
85-89	8.257318727887056E-7
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	1.975114483089731E-4
145-149	0.01921577963016298
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
60-61	1.0
62-63	1.0
64-65	5.0
66-67	2.0
68-69	5.0
70-71	11.0
72-73	11.0
74-75	13.0
76-77	34.0
78-79	31.0
80-81	37.0
82-83	73.0
84-85	61.0
86-87	92.0
88-89	129.0
90-91	122.0
92-93	110.0
94-95	112.0
96-97	141.0
98-99	151.0
100-101	166.0
102-103	156.0
104-105	193.0
106-107	176.0
108-109	189.0
110-111	185.0
112-113	205.0
114-115	209.0
116-117	234.0
118-119	282.0
120-121	328.0
122-123	320.0
124-125	376.0
126-127	414.0
128-129	428.0
130-131	558.0
132-133	758.0
134-135	1062.0
136-137	1537.0
138-139	3330.0
140-141	7054.0
142-143	10899.0
144-145	69660.0
146-147	827111.0
148-149	1006022.0
150-151	2.228828E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.78191769456613
#Duplication Level	Percentage of total
1	27.37951470735463
2	10.688448463888879
3	7.032509552661606
4	5.044027079387578
5	3.8525490436075795
6	3.1587715415909385
7	2.578881337083901
8	2.2004112882462348
9	1.8345720892970134
>10	21.772302840627223
>50	5.422654892462301
>100	6.72133374856059
>500	1.116213023095498
>1k	1.00322295563223
>5k	0.09448600014744374
>10k+	0.10010143635614444
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	8.257203976966694E-6	4.128601988483347E-6	0.0	0.0	0.0010280218951323535	0.0
2	1.651440795393339E-5	1.2385805965450042E-5	0.0	0.0	0.005268096137304751	0.0
3	4.954322386180017E-5	1.651440795393339E-5	0.0	0.0	0.006849350698893873	0.0
4	9.4957845735117E-5	1.651440795393339E-5	0.0	0.0	0.008310875802816978	0.0
5	9.908644772360033E-5	1.651440795393339E-5	0.0	0.0	0.0098425871405443	0.0
6	9.908644772360033E-5	1.651440795393339E-5	0.0	0.0	0.011460999120029772	0.0
7	1.1972945766601707E-4	4.1286019884833476E-5	0.0	0.0	0.013521171512282963	0.0
8	1.651440795393339E-4	4.541462187331682E-5	0.0	0.0	0.01908239839077003	0.0
9	1.651440795393339E-4	4.954322386180017E-5	0.0	0.0	0.0250151994482206	0.0
10-11	2.2294450737810075E-4	5.573612684452519E-5	0.0	0.0	0.03161683402780548	0.0
12-13	2.5390902229172584E-4	5.7800427838766865E-5	4.128601988483347E-6	0.0	0.037493898958411515	0.0
14-15	2.8487353720535094E-4	5.9864728833008534E-5	4.128601988483347E-6	0.0	0.045414621873316824	0.0
16-17	3.075808481420094E-4	6.19290298272502E-5	4.128601988483347E-6	0.0	0.05127517239596893	0.0
18-19	3.674455769750179E-4	7.225053479845858E-5	4.128601988483347E-6	2.0643009942416736E-6	0.05861789103248656	0.0
20-21	4.066672958656097E-4	8.67006417581503E-5	4.128601988483347E-6	6.192902982725021E-6	0.0645692707988853	0.0
22-23	4.8304643265255164E-4	9.908644772360033E-5	1.2385805965450042E-5	8.257203976966694E-6	0.07258495155952573	0.0
24-25	5.532326664567685E-4	1.0734365170056702E-4	1.2385805965450042E-5	8.257203976966694E-6	0.07811934252508765	0.0
26-27	6.151616962840188E-4	1.1972945766601707E-4	1.2385805965450042E-5	8.257203976966694E-6	0.08199609979227351	0.0
28-29	7.679199698579026E-4	1.238580596545004E-4	1.2385805965450042E-5	8.257203976966694E-6	0.08600084372110237	0.0
30-31	9.929287782302451E-4	1.2798666164298375E-4	1.2385805965450042E-5	8.257203976966694E-6	0.091541427589647	0.0
32-33	0.0011374298478271623	1.3624386561995045E-4	1.2385805965450042E-5	1.2385805965450042E-5	0.09783960992307836	0.0
34-35	0.0012427091985334877	1.3624386561995045E-4	1.2385805965450042E-5	1.2385805965450042E-5	0.10130969989439861	0.0
36-37	0.0013190883353204294	1.3624386561995045E-4	1.651440795393339E-5	1.2385805965450042E-5	0.10458368127126591	0.0
38-39	0.0013686315591822295	1.3830816661419213E-4	1.651440795393339E-5	1.2385805965450042E-5	0.10802280672767255	0.0
40-41	0.0014016603750900965	1.424367686026755E-4	1.651440795393339E-5	1.2385805965450042E-5	0.11070226941819825	0.0
42-43	0.0014243676860267549	1.5482257456812552E-4	1.651440795393339E-5	1.2385805965450042E-5	0.11339411791468937	2.0643009942416736E-6
44-45	0.0014594608029288633	1.7340128351630058E-4	1.651440795393339E-5	1.2385805965450042E-5	0.11557195546361435	4.128601988483347E-6
46-47	0.0015151969297733884	2.3326601234930912E-4	1.8578708948175065E-5	1.2385805965450042E-5	0.11806356676366404	4.128601988483347E-6
48-49	0.0015771259596006387	2.4358751732051748E-4	2.0643009942416738E-5	1.2385805965450042E-5	0.12025172581756022	6.192902982725021E-6
50-51	0.0016535050963875806	2.5390902229172584E-4	2.0643009942416738E-5	1.2385805965450042E-5	0.12224171197600919	8.257203976966694E-6
52-53	0.0017360771361572477	2.642305272629342E-4	2.0643009942416738E-5	1.651440795393339E-5	0.12423169813445817	8.257203976966694E-6
54-55	0.0017814917580305644	2.9725934317080104E-4	2.0643009942416738E-5	1.651440795393339E-5	0.12592442494973632	8.257203976966694E-6
56-57	0.0018454850888520564	3.240952560959428E-4	2.0643009942416738E-5	2.0643009942416738E-5	0.12768940229981296	8.257203976966694E-6
58-59	0.001884706807742648	3.3235246007290946E-4	2.0643009942416738E-5	2.0643009942416738E-5	0.12944199384392413	8.257203976966694E-6
60-61	0.0019631502455238316	3.447382660383595E-4	2.0643009942416738E-5	2.0643009942416738E-5	0.13078998239316397	8.257203976966694E-6
62-63	0.001983793255466248	3.5918837299805126E-4	2.0643009942416738E-5	2.0643009942416738E-5	0.13268501070587782	8.257203976966694E-6
64-65	0.0020085648673971485	3.674455769750179E-4	2.0643009942416738E-5	2.0643009942416738E-5	0.13433645150127116	8.257203976966694E-6
66-67	0.0021097156161149905	4.0253869387712636E-4	2.0643009942416738E-5	2.0643009942416738E-5	0.13549452435904075	1.8578708948175065E-5
68-69	0.002157194538982549	4.2318170381954313E-4	2.0643009942416738E-5	2.0643009942416738E-5	0.13664021141084487	2.0643009942416738E-5
70-71	0.002186094752901932	4.355675097849932E-4	2.0643009942416738E-5	2.0643009942416738E-5	0.1379345281342344	2.0643009942416738E-5
72-73	0.0022170592678155574	4.541462187331682E-4	2.0643009942416738E-5	2.0643009942416738E-5	0.13884488487269497	2.4771611930900087E-5
74-75	0.0022377022777579744	4.644677237043766E-4	2.4771611930900083E-5	2.0643009942416738E-5	0.13991006418572366	2.8900213919383432E-5
76-77	0.0022666024916773576	4.9336793762376E-4	2.4771611930900083E-5	2.0643009942416738E-5	0.141125937471332	2.8900213919383432E-5
78-79	0.0023285315215046077	5.222681515431435E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.14252966214741636	2.8900213919383432E-5
80-81	0.0023450459294585414	5.284610545258684E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.14391274381355829	2.8900213919383432E-5
82-83	0.002353303133435508	5.449754624798019E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.1455517988029862	2.8900213919383432E-5
84-85	0.0023698175413894413	5.491040644682852E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.14708144583971924	3.302881590786678E-5
86-87	0.002386331949343375	5.552969674510102E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.14877004405300892	3.302881590786678E-5
88-89	0.002398717755308825	5.718113754049437E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.15040909904243682	3.7157417896350124E-5
90-91	0.002409039260280033	6.027758903185687E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.15123894804212198	3.7157417896350124E-5
92-93	0.002411103561274275	6.06904492307052E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.15216375488754225	3.7157417896350124E-5
94-95	0.002421425066245483	6.13097395289777E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.15315461936477825	3.7157417896350124E-5
96-97	0.0024234893672397248	6.213545992667438E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.15404226879230215	4.1286019884833476E-5
98-99	0.002427617969228208	6.234189002609855E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.1549195967148549	4.1286019884833476E-5
100-101	0.0024317465712166914	6.254832012552271E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.15565242356781067	4.747892286755849E-5
102-103	0.002437939474199417	6.337404052321938E-4	2.8900213919383432E-5	2.0643009942416738E-5	0.15635222160485862	5.367182585028351E-5
104-105	0.002448260979170625	6.378690072206772E-4	2.8900213919383432E-5	2.4771611930900083E-5	0.15716142759460133	5.367182585028351E-5
106-107	0.0024812897950784916	6.564477161688522E-4	2.8900213919383432E-5	2.4771611930900083E-5	0.15789425444755714	5.573612684452519E-5
108-109	0.002485418397066975	6.688335221343023E-4	2.8900213919383432E-5	2.4771611930900083E-5	0.15869726753431715	5.9864728833008534E-5
110-111	0.0024957399020381836	6.812193280997523E-4	3.302881590786678E-5	2.4771611930900083E-5	0.15955808104891595	6.605763181573356E-5
112-113	0.002506061407009392	6.89476532076719E-4	3.302881590786678E-5	2.4771611930900083E-5	0.1602702648919293	6.812193280997524E-5
114-115	0.0025081257080036335	6.977337360536857E-4	3.302881590786678E-5	2.4771611930900083E-5	0.1609349698120751	7.225053479845858E-5
116-117	0.002510190008997875	7.121838430133774E-4	3.509311690210845E-5	2.4771611930900083E-5	0.16177514031673149	7.637913678694192E-5
118-119	0.002512254309992117	7.266339499730691E-4	3.7157417896350124E-5	2.4771611930900083E-5	0.16255131749056634	7.84434377811836E-5
120-121	0.0025163829119806	7.348911539500359E-4	3.7157417896350124E-5	2.4771611930900083E-5	0.16325937273159125	8.257203976966695E-5
122-123	0.0025205115139690835	7.410840569327608E-4	3.7157417896350124E-5	2.4771611930900083E-5	0.16392407765173705	8.876494275239196E-5
124-125	0.0025267044169518084	7.472769599154859E-4	3.7157417896350124E-5	2.6835912925141756E-5	0.16481172707926098	9.289354474087533E-5
126-127	0.0025432188249057417	7.823700768175943E-4	3.7157417896350124E-5	2.8900213919383432E-5	0.1658376846733991	1.01150748717842E-4
128-129	0.0025576689318654337	7.885629798003193E-4	3.7157417896350124E-5	2.8900213919383432E-5	0.16674184850887697	1.1560085567753373E-4
130-131	0.002567990436836642	8.030130867600111E-4	3.7157417896350124E-5	2.8900213919383432E-5	0.16763775514037782	1.238580596545004E-4
132-133	0.002588633446779059	8.09205989742736E-4	3.7157417896350124E-5	2.8900213919383432E-5	0.16846553983906876	1.3005096263722546E-4
134-135	0.0026030835537387506	8.09205989742736E-4	4.1286019884833476E-5	3.302881590786678E-5	0.16920249529401304	1.3624386561995045E-4
136-137	0.002613405058709959	8.15398892725461E-4	4.335032087907515E-5	3.7157417896350124E-5	0.17001376558475	1.3624386561995045E-4
138-139	0.002617533660698442	8.277846986909111E-4	4.541462187331682E-5	3.7157417896350124E-5	0.17073833523372883	1.3624386561995045E-4
>>END_MODULE
