##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.2d.S.1.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24154014
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	69-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.749761882227936	37.0	35.0	39.0	34.0	39.0
2	35.475821741264205	36.0	35.0	38.0	33.0	39.0
3	35.759129931778624	36.0	35.0	38.0	34.0	39.0
4	35.76556111957209	37.0	35.0	38.0	33.0	39.0
5	35.81132589390732	36.0	35.0	38.0	33.0	39.0
6	35.68667928237518	36.0	35.0	38.0	33.0	39.0
7	35.68222246621203	36.0	35.0	38.0	33.0	39.0
8	35.757796985627316	36.0	35.0	38.0	33.0	39.0
9	35.847687055244734	37.0	35.0	39.0	33.0	39.0
10-14	35.7005824125133	36.4	35.0	38.4	33.0	39.0
15-19	35.65029389318065	36.0	35.0	38.0	33.0	39.0
20-24	35.626964379502304	36.0	35.0	38.0	33.0	39.0
25-29	35.59527522837406	36.0	35.0	38.0	33.0	39.0
30-34	35.566657144439844	36.0	35.0	38.0	33.0	39.0
35-39	35.562821359629915	36.0	35.0	38.0	33.0	39.0
40-44	35.564697784807116	36.0	35.0	38.0	33.0	39.0
45-49	35.46418748453156	36.0	35.0	38.0	32.6	39.0
50-54	35.47584533154613	36.0	34.8	38.0	32.8	39.0
55-59	35.50482059006838	36.0	35.0	38.0	32.6	39.0
60-64	35.43204495120356	36.0	34.8	38.0	32.0	39.0
65-69	35.401350491889254	36.0	34.4	38.0	32.0	39.0
70-74	35.38550011826746	36.0	34.0	38.0	32.0	39.0
75-79	35.29567494616382	36.0	34.0	38.0	32.0	39.0
80-84	35.25372294096944	36.0	34.0	38.0	32.0	39.0
85-89	35.18417420049217	36.0	34.0	38.0	31.8	39.0
90-94	35.10101930137043	36.0	34.0	38.0	31.2	39.0
95-99	34.993658857735724	36.0	34.0	38.0	31.0	39.0
100-104	34.93050744253331	36.0	34.0	38.0	30.8	39.0
105-109	34.66354095568259	36.0	34.0	38.0	29.8	39.0
110-114	34.50338979070022	36.0	34.0	38.0	29.4	39.0
115-119	34.40039255091521	36.0	34.0	38.0	29.0	39.0
120-124	34.366530744409	36.0	34.0	38.0	28.8	39.0
125-129	34.32386165852681	35.8	34.0	38.0	28.6	39.0
130-134	34.2542912360161	35.4	34.0	38.0	28.4	39.0
135-139	34.15805100481166	35.0	34.0	38.0	28.0	39.0
140-144	33.99211070849659	35.0	33.8	38.0	27.4	39.0
145-149	34.05701741234297	35.0	34.0	38.0	27.6	39.0
150	35.1943130018542	36.0	34.0	38.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	11.0
21	118.0
22	1244.0
23	7329.0
24	25616.0
25	61325.0
26	119036.0
27	207794.0
28	323916.0
29	461173.0
30	629208.0
31	845043.0
32	1149557.0
33	1640646.0
34	2491313.0
35	4136323.0
36	9599655.0
37	2454704.0
38	3.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.60001306615124	18.206940676609694	14.690866702321195	32.50217955491787
2	24.141743065976527	25.627843885492492	33.01099767516902	17.21941537336196
3	19.7133942209357	29.032652709400598	29.689301331033423	21.564651738630275
4	23.631388969137802	33.91840793004426	21.729067475078885	20.72113562573906
5	25.428506417194257	34.807465127742326	21.83203172772857	17.931996727334845
6	19.109560837382972	38.583152266120244	22.259231943808594	20.048054952688197
7	20.501052123262	20.17933333979189	37.16292041562947	22.156694121316647
8	21.662772075895955	23.129219847268452	28.260785143206423	26.947222933629167
9	23.58395585926215	23.184096854460712	27.886938378026937	25.3450089082502
10-14	23.31344761164749	28.393908358254656	25.296392558189297	22.99625147190856
15-19	23.247568706385614	27.393007224389287	26.86189053297725	22.497533536247847
20-24	23.196265432321102	27.320387410556275	26.769999388093424	22.713347769029195
25-29	23.253526308298074	26.994494579658685	26.759968757159786	22.99201035488346
30-34	23.1020541761713	26.734136197817886	26.979277233175402	23.18453239283541
35-39	23.37543151212879	26.521660540562742	27.039114906532717	23.06379304077575
40-44	23.57154301558325	26.15832382973695	27.063158115251568	23.20697503942823
45-49	23.460188439072695	26.002471473271484	27.133964565889546	23.40337552176628
50-54	23.693467263867614	26.068930820359714	26.8311320842987	23.406469831473974
55-59	23.73699626074573	26.075896950295714	26.860557421221998	23.32654936773656
60-64	23.44272467507885	26.07645503558953	26.94105087460825	23.539769414723366
65-69	23.65161252287094	25.997731888372673	26.947813311692208	23.402842277064178
70-74	23.790204173803094	26.003357223141947	26.649553869515724	23.556884733539228
75-79	23.63846429404979	26.09812378108667	26.88901079373466	23.374401131128884
80-84	23.940245007393194	26.104433785508924	26.59829059121349	23.35703061588439
85-89	23.711815346530383	26.159963565643206	26.804307404502026	23.323913683324385
90-94	23.729167113111597	25.966501245502695	26.80981349934869	23.49451814203702
95-99	23.752818715669232	26.140646236005622	26.82025389423443	23.28628115409072
100-104	23.991064035034718	25.965770066276146	26.74669611062414	23.296469788064996
105-109	23.78640585357471	26.20830771683909	26.603974282116276	23.401312147469923
110-114	23.891413007393663	26.311188662796187	26.475801510574605	23.321596819235555
115-119	24.019563696307863	26.425102240756516	26.647073521927016	22.9082605410086
120-124	23.99110987121335	26.571202360626057	26.74471203973071	22.692975728429882
125-129	23.963898408379617	26.622294661317024	26.597034197987952	22.816772732315407
130-134	24.096981403297416	26.63860433516514	26.628742858432602	22.63567140310484
135-139	23.876010170339796	26.60420603251249	26.931000697352932	22.588783099794785
140-144	23.9371879344759	26.7717804000504	26.643016647313743	22.648015018159953
145-149	23.812491429413164	26.794865520077487	26.600999986581126	22.79164306392822
150	24.789714995562466	25.163964402709116	26.30037079367056	23.745949808057862
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	1.5
9	2.0
10	2.0
11	4.0
12	7.5
13	13.0
14	18.5
15	24.5
16	34.5
17	76.0
18	134.5
19	217.5
20	356.5
21	518.5
22	787.5
23	1315.0
24	2237.0
25	3713.5
26	5740.0
27	8965.5
28	13726.5
29	19854.5
30	28778.5
31	42152.5
32	61653.5
33	86241.5
34	119464.5
35	171702.0
36	246663.0
37	339744.5
38	457263.0
39	599580.5
40	770035.5
41	959717.0
42	1153449.5
43	1351537.5
44	1507520.0
45	1603489.5
46	1638306.5
47	1618199.5
48	1574554.0
49	1518574.0
50	1407550.0
51	1248056.5
52	1070529.5
53	899617.0
54	762989.5
55	658671.5
56	545010.0
57	426663.0
58	350735.5
59	287951.0
60	215425.0
61	143374.5
62	90386.0
63	57374.5
64	36251.0
65	18758.5
66	10618.5
67	6438.5
68	4265.5
69	2826.5
70	1424.0
71	951.0
72	634.0
73	424.0
74	309.0
75	207.0
76	134.0
77	60.0
78	29.0
79	14.5
80	9.0
81	4.5
82	2.5
83	1.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	8.280234466762464E-7
90-94	0.0
95-99	0.0
100-104	0.0
105-109	8.280748030111483E-7
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
68-69	2.0
70-71	1.0
72-73	5.0
74-75	9.0
76-77	6.0
78-79	9.0
80-81	14.0
82-83	22.0
84-85	24.0
86-87	32.0
88-89	47.0
90-91	65.0
92-93	62.0
94-95	88.0
96-97	131.0
98-99	189.0
100-101	201.0
102-103	244.0
104-105	297.0
106-107	299.0
108-109	375.0
110-111	316.0
112-113	308.0
114-115	376.0
116-117	379.0
118-119	418.0
120-121	401.0
122-123	512.0
124-125	483.0
126-127	551.0
128-129	717.0
130-131	879.0
132-133	1153.0
134-135	1662.0
136-137	2371.0
138-139	5690.0
140-141	10826.0
142-143	13648.0
144-145	70836.0
146-147	782127.0
148-149	1410509.0
150-151	2.184773E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.27508333650359
#Duplication Level	Percentage of total
1	21.515797807684947
2	9.509697820335575
3	5.990715140576999
4	4.285762238897842
5	3.0915831879393165
6	2.618082274909952
7	2.075034504819799
8	1.7879415088649486
9	1.5953479193456217
>10	19.19254043254255
>50	6.473584020485094
>100	12.366846076283068
>500	3.2976887859842936
>1k	4.896130442352301
>5k	0.6917603401967178
>10k+	0.611487498781093
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	29003	0.12007528024120545	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.7738660745994434E-4	0.0	4.14009861880514E-6	0.0	0.0017429815185169637	0.0
2	0.0013538122483492806	4.14009861880514E-6	4.14009861880514E-6	0.0	0.008391979900318017	0.0
3	0.002020368125976908	4.14009861880514E-6	4.14009861880514E-6	0.0	0.012445136448128249	0.0
4	0.004827354989526793	4.14009861880514E-6	4.14009861880514E-6	0.0	0.01886642940589502	0.0
5	0.0050633406107986854	4.14009861880514E-6	4.14009861880514E-6	0.0	0.022911305756467642	0.0
6	0.005978302405554622	4.14009861880514E-6	4.14009861880514E-6	0.0	0.0298873719291543	4.14009861880514E-6
7	0.007915868559155426	8.28019723761028E-6	4.14009861880514E-6	0.0	0.040506724886389484	4.14009861880514E-6
8	0.014606267927144532	8.28019723761028E-6	4.14009861880514E-6	0.0	0.05382128204446681	4.14009861880514E-6
9	0.015198302029633667	8.28019723761028E-6	4.14009861880514E-6	0.0	0.06297918018926378	4.14009861880514E-6
10-11	0.015856577710023682	8.28019723761028E-6	4.14009861880514E-6	0.0	0.10090869368544707	4.14009861880514E-6
12-13	0.016624566003812038	8.28019723761028E-6	4.14009861880514E-6	0.0	0.11781892649395666	4.14009861880514E-6
14-15	0.02006498795603911	1.656039447522056E-5	4.14009861880514E-6	8.28019723761028E-6	0.1258155269761788	4.14009861880514E-6
16-17	0.023693784395421812	1.656039447522056E-5	4.14009861880514E-6	8.28019723761028E-6	0.13190354199513174	4.14009861880514E-6
18-19	0.030150268191448427	1.656039447522056E-5	4.14009861880514E-6	8.28019723761028E-6	0.13856289062347982	6.21014792820771E-6
20-21	0.03530055087324202	2.6910641022233407E-5	4.14009861880514E-6	8.28019723761028E-6	0.1430693879700492	8.28019723761028E-6
22-23	0.03750101328913695	3.7260887569246255E-5	6.21014792820771E-6	8.28019723761028E-6	0.14737716058291594	8.28019723761028E-6
24-25	0.04310670681899911	4.76111341162591E-5	8.28019723761028E-6	8.28019723761028E-6	0.1515358896455057	8.28019723761028E-6
26-27	0.04821972861322346	6.624157790088224E-5	8.28019723761028E-6	8.28019723761028E-6	0.1545747220317087	8.28019723761028E-6
28-29	0.06087394004160136	6.624157790088224E-5	1.2420295856415418E-5	1.0350246547012848E-5	0.15739412919111498	8.28019723761028E-6
30-31	0.0844186808867462	6.624157790088224E-5	1.2420295856415418E-5	1.2420295856415418E-5	0.16057165488104794	8.28019723761028E-6
32-33	0.10242189973062035	7.245172582908993E-5	1.2420295856415418E-5	1.2420295856415418E-5	0.16295842173478908	8.28019723761028E-6
34-35	0.1147800941077537	7.866187375729765E-5	1.2420295856415418E-5	1.2420295856415418E-5	0.16518786484101566	8.28019723761028E-6
36-37	0.11646925434422618	7.866187375729765E-5	1.2420295856415418E-5	1.2420295856415418E-5	0.16676938251339923	8.28019723761028E-6
38-39	0.11831987842683209	8.073192306670021E-5	1.2420295856415418E-5	1.2420295856415418E-5	0.16798657150732793	8.28019723761028E-6
40-41	0.1195515577659266	8.280197237610278E-5	1.2420295856415418E-5	1.2420295856415418E-5	0.16921411074780365	1.2420295856415418E-5
42-43	0.11982894437338655	8.280197237610278E-5	1.2420295856415418E-5	1.2420295856415418E-5	0.17055964279891533	1.4490345165817988E-5
44-45	0.12068594478747921	8.487202168550536E-5	1.2420295856415418E-5	1.2420295856415418E-5	0.1716774694259927	1.656039447522056E-5
46-47	0.12184103230212585	9.315221892311563E-5	1.2420295856415418E-5	1.656039447522056E-5	0.17274975496826322	1.656039447522056E-5
48-49	0.12288019705544595	1.0971261339833619E-4	1.2420295856415418E-5	1.656039447522056E-5	0.17370197765058842	1.656039447522056E-5
50-51	0.1237351274202292	1.159227613265439E-4	1.2420295856415418E-5	1.656039447522056E-5	0.17464592013567598	2.0700493094025696E-5
52-53	0.12468321000393558	1.221329092547516E-4	1.2420295856415418E-5	1.656039447522056E-5	0.17557537227559775	2.0700493094025696E-5
54-55	0.12515311119716996	1.3869330372997217E-4	1.2420295856415418E-5	1.656039447522056E-5	0.17643858283761862	2.0700493094025696E-5
56-57	0.12586520815960445	1.4076335303937475E-4	1.2420295856415418E-5	1.656039447522056E-5	0.17735561468168398	2.0700493094025696E-5
58-59	0.1259832009702404	1.4283340234877732E-4	1.4490345165817988E-5	1.656039447522056E-5	0.17815879381373217	2.2770542403428264E-5
60-61	0.12629163831734136	1.4904355027698502E-4	1.656039447522056E-5	1.656039447522056E-5	0.17878187865586234	2.8980690331635975E-5
62-63	0.1266331964533928	1.5939379682399787E-4	1.656039447522056E-5	1.656039447522056E-5	0.17955814714688828	2.8980690331635975E-5
64-65	0.1266994380312937	1.7181409268041326E-4	1.656039447522056E-5	1.8630443784623127E-5	0.18027231415863218	2.8980690331635975E-5
66-67	0.1267905202009074	1.842343885368287E-4	1.656039447522056E-5	2.0700493094025696E-5	0.18087055840904953	2.8980690331635975E-5
68-69	0.12684020138433305	1.8630443784623126E-4	1.656039447522056E-5	2.0700493094025696E-5	0.1815122736949643	2.8980690331635975E-5
70-71	0.1269499139977314	1.8837448715563384E-4	1.656039447522056E-5	2.0700493094025696E-5	0.18211465804400048	3.105073964103854E-5
72-73	0.12701615557563228	1.9458463508384156E-4	1.656039447522056E-5	2.4840591712830835E-5	0.1826114698782571	3.312078895044112E-5
74-75	0.12706583675905794	2.0079478301204926E-4	1.656039447522056E-5	2.4840591712830835E-5	0.18315175274801115	3.312078895044112E-5
76-77	0.12709481744938955	2.0286483232145183E-4	2.0700493094025696E-5	2.4840591712830835E-5	0.1838203786749482	3.312078895044112E-5
78-79	0.12713414838626821	2.0700493094025696E-4	2.0700493094025696E-5	2.4840591712830835E-5	0.1846960095328255	3.312078895044112E-5
80-81	0.1272024600134785	2.1321507886846468E-4	2.0700493094025696E-5	2.4840591712830835E-5	0.18545364758006683	3.312078895044112E-5
82-83	0.12726249144345117	2.1528512817786726E-4	2.0700493094025696E-5	2.6910641022233407E-5	0.18628580740244666	3.933093687864882E-5
84-85	0.12730389242963924	2.1942522679667238E-4	2.0700493094025696E-5	2.8980690331635975E-5	0.18699790436488112	4.140098618805139E-5
86-87	0.1273597837609931	2.256353747248801E-4	2.6910641022233407E-5	2.8980690331635975E-5	0.187691370883531	4.3471035497453966E-5
88-89	0.12739911469787174	2.2770542403428268E-4	2.8980690331635975E-5	2.8980690331635975E-5	0.18831859582427996	4.5541084806856534E-5
90-91	0.127436375585441	2.3598562127189295E-4	2.8980690331635975E-5	2.8980690331635975E-5	0.18873674578477928	4.76111341162591E-5
92-93	0.1274487958812974	2.442658185095032E-4	2.8980690331635975E-5	2.8980690331635975E-5	0.18926460835867695	4.968118342566167E-5
94-95	0.12746742632508204	2.546160650565161E-4	2.8980690331635975E-5	3.105073964103854E-5	0.18984836226392848	5.589133135386938E-5
96-97	0.12748191667024783	2.6496631160352895E-4	3.312078895044112E-5	3.312078895044112E-5	0.19036794464058854	5.796138066327195E-5
98-99	0.1275067572619607	2.6496631160352895E-4	3.312078895044112E-5	3.312078895044112E-5	0.19079437479832545	6.624157790088224E-5
100-101	0.1275295278043641	2.7117645953173664E-4	3.5190838259843687E-5	3.312078895044112E-5	0.19124771559708462	7.452177513849251E-5
102-103	0.12754608819883934	2.794566567693469E-4	3.7260887569246255E-5	3.312078895044112E-5	0.19169070614929676	7.452177513849251E-5
104-105	0.12757506888917097	2.8980690331635974E-4	4.140098618805139E-5	3.312078895044112E-5	0.1921295566028901	7.866187375729765E-5
106-107	0.1276081896781214	2.8980690331635974E-4	4.140098618805139E-5	3.312078895044112E-5	0.19256840705648345	8.280197237610278E-5
108-109	0.12767029115740347	2.8980690331635974E-4	4.5541084806856534E-5	3.7260887569246255E-5	0.19304037829902723	9.108216961371307E-5
110-111	0.12771169214359152	2.8980690331635974E-4	4.5541084806856534E-5	3.7260887569246255E-5	0.19352062973880862	1.014324161607259E-4
112-113	0.12774274288323256	2.8980690331635974E-4	4.5541084806856534E-5	3.7260887569246255E-5	0.19403193191823107	1.159227613265439E-4
114-115	0.12776965352425482	2.918769526257623E-4	4.5541084806856534E-5	3.7260887569246255E-5	0.19442731133632696	1.2627300787355675E-4
116-117	0.12779035401734884	2.960170512445675E-4	4.5541084806856534E-5	3.7260887569246255E-5	0.19484132119820746	1.3869330372997217E-4
118-119	0.12781312455975227	3.0636729779158034E-4	4.968118342566167E-5	3.7260887569246255E-5	0.19533606298315467	1.5525369820519272E-4
120-121	0.1278338250528463	3.1050739641038543E-4	5.3821282044466814E-5	4.140098618805139E-5	0.19592188693771562	1.5939379682399787E-4
122-123	0.12785452554594032	3.1878759364799573E-4	5.3821282044466814E-5	4.140098618805139E-5	0.19635452724338076	1.6146384613340044E-4
124-125	0.12787108594041552	3.208576429573983E-4	5.3821282044466814E-5	4.140098618805139E-5	0.19686789947211258	1.6353389544280302E-4
126-127	0.12790213668005657	3.229276922668009E-4	5.3821282044466814E-5	4.140098618805139E-5	0.19738955189808205	1.6560394475220556E-4
128-129	0.1279207671238412	3.249977415762035E-4	5.3821282044466814E-5	4.140098618805139E-5	0.1978511728940788	1.7181409268041326E-4
130-131	0.12792904732107882	3.3534798812321633E-4	5.589133135386938E-5	4.140098618805139E-5	0.19847425773620897	1.842343885368287E-4
132-133	0.12794767776486343	3.436281853608266E-4	6.003142997267452E-5	4.140098618805139E-5	0.1989565792252998	2.0286483232145183E-4
134-135	0.1279807985538139	3.436281853608266E-4	6.210147928207709E-5	4.140098618805139E-5	0.19938507943234612	2.0907498024965953E-4
136-137	0.12800149904690789	3.456982346702292E-4	6.210147928207709E-5	4.3471035497453966E-5	0.19990052171038736	2.1528512817786726E-4
138-139	0.1280035690962173	3.539784319078394E-4	6.210147928207709E-5	4.5541084806856534E-5	0.20037042290362173	2.256353747248801E-4
>>END_MODULE
