##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.2d.S.2.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20724830
Total Bases	3.1 Gbp
Sequences flagged as poor quality	0
Sequence length	64-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.93291824347896	37.0	35.0	38.0	33.0	39.0
2	35.175036128161246	35.0	34.0	37.0	32.0	39.0
3	35.34804589470698	35.0	34.0	37.0	33.0	39.0
4	35.40215022270388	36.0	35.0	37.0	33.0	39.0
5	35.325318084635676	35.0	34.0	37.0	33.0	39.0
6	35.272413428722935	35.0	34.0	37.0	33.0	39.0
7	35.33207037162669	35.0	34.0	37.0	33.0	39.0
8	35.427503434286315	36.0	34.0	38.0	33.0	39.0
9	35.42859926957181	36.0	34.0	38.0	33.0	39.0
10-14	35.39787595845176	35.8	34.2	37.8	33.0	39.0
15-19	35.372193248388534	35.8	34.0	38.0	33.0	39.0
20-24	35.345994432764954	36.0	34.0	38.0	32.6	39.0
25-29	35.33928037045418	36.0	34.0	38.0	32.0	39.0
30-34	35.32713362666907	36.0	34.0	38.0	32.0	39.0
35-39	35.298743092223184	36.0	34.0	38.0	32.0	39.0
40-44	35.288673180913904	36.0	34.0	38.0	32.0	39.0
45-49	35.26247868860686	36.0	34.0	38.0	32.0	39.0
50-54	35.24335018429584	36.0	34.0	38.0	32.0	39.0
55-59	35.218349873074956	36.0	34.0	38.0	32.0	39.0
60-64	35.18000179494838	36.0	34.0	38.0	32.0	39.0
65-69	35.144848501553994	36.0	34.0	38.0	32.0	39.0
70-74	35.13061681583823	36.0	34.0	38.0	32.0	39.0
75-79	35.07519934141946	35.4	34.0	38.0	32.0	39.0
80-84	35.007922887082444	35.4	34.0	38.0	31.4	39.0
85-89	34.994241001102665	35.0	34.0	38.0	31.0	39.0
90-94	34.950508202734454	35.0	34.0	38.0	31.0	39.0
95-99	34.88625293665409	35.0	34.0	37.2	31.0	39.0
100-104	34.8287195898694	35.0	34.0	37.0	31.0	39.0
105-109	34.77543407640555	35.0	34.0	37.0	30.8	39.0
110-114	34.710120643764206	35.0	34.0	37.0	30.0	39.0
115-119	34.64819068327209	35.0	34.0	37.0	30.0	39.0
120-124	34.58097239486373	35.0	34.0	37.0	30.0	39.0
125-129	34.50673830349526	35.0	34.0	37.0	30.0	39.0
130-134	34.434034014900405	35.0	34.0	37.0	30.0	39.0
135-139	34.359756591258915	35.0	34.0	37.0	29.6	39.0
140-144	34.27564692555757	35.0	34.0	37.0	29.0	39.0
145-149	34.24676421699452	35.0	34.0	37.0	29.0	39.0
150	35.05058875634755	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	148.0
22	1582.0
23	8344.0
24	28625.0
25	70529.0
26	138632.0
27	239193.0
28	351090.0
29	461083.0
30	588744.0
31	757349.0
32	998635.0
33	1370866.0
34	2015770.0
35	3344718.0
36	8452919.0
37	1896591.0
38	4.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.19852080813208	19.461119825832103	12.76223254907278	34.578126816963035
2	23.69799528864629	27.03677313815231	33.337061550664664	15.928170022536737
3	18.500335105281927	30.4313762766691	28.97168275927957	22.096605858769408
4	22.423802752543686	35.09938079106077	21.6561197365672	20.82069671982834
5	24.60086765488547	35.47225236588189	21.94067213096561	17.98620784826703
6	18.488335006849272	38.735381665374334	22.67950086924718	20.096782458529212
7	19.87800141183305	20.763002639828652	36.87644723744417	22.48254871089413
8	20.7597891032158	23.41731633021839	28.69116417360239	27.131730392963416
9	22.852496256905365	23.772194995085606	28.12121981217699	25.25408893583204
10-14	22.87930853956341	28.79567166534056	25.17683377861242	23.14818601648361
15-19	22.847676172472113	27.8750062400995	26.635241173367803	22.64207641406059
20-24	22.837458989771132	27.686148755140806	26.615924254688366	22.8604680003997
25-29	22.838293004092193	27.296440067300914	26.643882724249124	23.22138420435777
30-34	22.78937412552358	26.960282202172774	26.804158362738402	23.446185309565244
35-39	23.064328151304498	26.67250443067567	26.893625665445747	23.369541752574087
40-44	23.286273518287	26.20760122037189	26.883325942842472	23.622799318498632
45-49	23.152108847213704	26.030638610787154	26.95102637753844	23.866226164460695
50-54	23.45820062215227	26.060243678717747	26.626486200369314	23.855069498760663
55-59	23.464942293857174	26.135760824093612	26.581641441690955	23.81765544035826
60-64	23.191412426543426	26.077896899516183	26.667613678857677	24.06307699508271
65-69	23.435472721492395	25.985109223194165	26.64787175467524	23.9315463006382
70-74	23.60937628861174	25.967733726533957	26.34339049692623	24.07949948792807
75-79	23.430215093302778	26.112453299201565	26.599559042092153	23.857772565403508
80-84	23.747082066699093	26.118707000973785	26.28327081182316	23.85094012050396
85-89	23.47496965583895	26.216965275085414	26.49026272699616	23.81780234207948
90-94	23.452808336867847	25.962262357074117	26.556006758976793	24.028922547081244
95-99	23.535933494185933	26.090510758151535	26.559834986154595	23.813720761507938
100-104	23.745714885862082	25.962700580051973	26.502452266598663	23.78913226748728
105-109	23.537235060223182	26.146988168235126	26.35528446158908	23.960492309952613
110-114	23.641245444759782	26.264262613452217	26.259174789965872	23.83531715182213
115-119	23.83105815609933	26.362690507809894	26.415439764865972	23.390811571224802
120-124	23.785609497798198	26.56877376407003	26.499304316300222	23.14631242183155
125-129	23.73170489038895	26.60883567993984	26.37333038985098	23.28612903982023
130-134	23.89116423427871	26.652227314431336	26.371491623035947	23.08511682825401
135-139	23.624744673155384	26.61141914467167	26.695790323263168	23.068045858909773
140-144	23.715525569152398	26.791648608588346	26.428636929166537	23.064188893092716
145-149	23.482696184071507	26.871996156316403	26.43478610434476	23.210521555267338
150	23.744975346189573	25.724212913820548	26.697951634978427	23.832860105011452
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	2.5
2	8.5
3	17.5
4	26.0
5	24.0
6	17.5
7	14.5
8	10.5
9	8.5
10	7.0
11	5.0
12	5.5
13	9.5
14	15.0
15	23.5
16	38.0
17	86.5
18	169.5
19	257.0
20	370.5
21	552.0
22	863.0
23	1393.5
24	2259.0
25	3572.0
26	5553.5
27	8585.5
28	13060.0
29	18701.0
30	26492.0
31	38328.5
32	54872.0
33	76076.0
34	104584.5
35	149563.5
36	213625.0
37	290360.0
38	387924.0
39	508643.5
40	651608.5
41	807447.5
42	972376.5
43	1140548.5
44	1269941.5
45	1358168.5
46	1395824.5
47	1378209.0
48	1346609.5
49	1303944.5
50	1212942.5
51	1084143.5
52	934124.0
53	790597.0
54	676774.0
55	588654.5
56	485726.5
57	372122.5
58	302875.0
59	247884.5
60	184045.0
61	124948.5
62	78331.5
63	47811.0
64	29181.5
65	14453.5
66	7831.5
67	4437.0
68	2861.0
69	1858.5
70	823.5
71	563.0
72	357.0
73	257.5
74	210.0
75	132.5
76	77.5
77	25.0
78	8.5
79	0.5
80	0.5
81	0.5
82	0.0
83	0.0
84	0.5
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.825130049317654E-6
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	9.65026009863531E-7
20-24	0.02471045600856557
25-29	0.0
30-34	9.65026009863531E-7
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	5.307648381148908E-4
80-84	0.04721592008071262
85-89	1.9300598983474122E-6
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	9.652458874613062E-7
140-144	2.8973323054242166E-4
145-149	0.026731144449115587
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
64-65	1.0
66-67	0.0
68-69	2.0
70-71	5.0
72-73	2.0
74-75	8.0
76-77	6.0
78-79	6.0
80-81	9.0
82-83	15.0
84-85	18.0
86-87	20.0
88-89	36.0
90-91	24.0
92-93	36.0
94-95	54.0
96-97	49.0
98-99	62.0
100-101	56.0
102-103	64.0
104-105	91.0
106-107	81.0
108-109	90.0
110-111	108.0
112-113	103.0
114-115	122.0
116-117	115.0
118-119	137.0
120-121	147.0
122-123	173.0
124-125	207.0
126-127	261.0
128-129	323.0
130-131	380.0
132-133	507.0
134-135	702.0
136-137	1191.0
138-139	3667.0
140-141	6801.0
142-143	8581.0
144-145	53683.0
146-147	684136.0
148-149	951491.0
150-151	1.901126E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.41009488704695
#Duplication Level	Percentage of total
1	24.793228457798648
2	8.619266557322659
3	5.283325804886921
4	3.6748243871342554
5	2.782932745058917
6	2.2532201252929034
7	1.850432795759872
8	1.5238590348631738
9	1.302707781335303
>10	17.71151704383452
>50	6.20076438797307
>100	12.445846256125654
>500	3.812183561930365
>1k	5.646132124768237
>5k	1.2269001935831934
>10k+	0.8728587423323954
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	32859	0.15854894829052882	No Hit
GAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCCG	24150	0.11652689069102135	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	3.0880832315632986E-4	4.825130049317654E-6	0.0	0.0	0.0022967619034752035	0.0
2	0.001408937974400755	1.4475390147952962E-5	0.0	0.0	0.010620111238548157	0.0
3	0.0022726362532286153	1.4475390147952962E-5	0.0	0.0	0.01540664024747127	0.0
4	0.005548899556715303	1.4475390147952962E-5	0.0	0.0	0.02349355821012766	0.0
5	0.0057370796286386905	1.4475390147952962E-5	0.0	0.0	0.028395890340234396	0.0
6	0.006851684670031069	1.4475390147952962E-5	0.0	0.0	0.03716797676989389	0.0
7	0.009384877945922838	1.4475390147952962E-5	0.0	0.0	0.04866143654736854	0.0
8	0.018653952770662053	1.4475390147952962E-5	0.0	0.0	0.06241305718792386	0.0
9	0.019430798708602193	1.9300520197270616E-5	0.0	0.0	0.0724010763900114	0.0
10-11	0.020251070816986197	1.9300520197270616E-5	0.0	0.0	0.11791652814522484	0.0
12-13	0.021008616234729067	1.9300520197270616E-5	0.0	0.0	0.1386042732316743	0.0
14-15	0.024318655448560975	1.9300520197270616E-5	0.0	0.0	0.15006636966382836	0.0
16-17	0.02806054380180682	1.9300520197270616E-5	0.0	4.825130049317654E-6	0.15828356613781633	0.0
18-19	0.034863977171344714	2.1713085221929442E-5	0.0	4.825130049317654E-6	0.16617506633347534	0.0
20-21	0.04023434691623526	2.412565024658827E-5	2.412565024658827E-6	7.237695073976481E-6	0.17163711354930294	0.0
22-23	0.04254558420985842	2.8950780295905925E-5	4.825130049317654E-6	9.650260098635308E-6	0.17717395028089494	0.0
24-25	0.048178923542436775	3.136334532056475E-5	4.825130049317654E-6	9.650260098635308E-6	0.1824092163844046	0.0
26-27	0.053045067197173626	3.377591034522358E-5	4.825130049317654E-6	9.650260098635308E-6	0.18661914235243426	0.0
28-29	0.06656266903033704	3.377591034522358E-5	4.825130049317654E-6	9.650260098635308E-6	0.19067466415888573	0.0
30-31	0.09192837769959994	4.342617044385889E-5	9.650260098635308E-6	9.650260098635308E-6	0.19498109272790176	0.0
32-33	0.11227112598752317	4.5838735468517716E-5	9.650260098635308E-6	9.650260098635308E-6	0.19794854770823211	0.0
34-35	0.12664760096946515	4.825130049317654E-5	9.650260098635308E-6	9.650260098635308E-6	0.20123928640186672	0.0
36-37	0.12876100793106626	4.825130049317654E-5	9.650260098635308E-6	9.650260098635308E-6	0.2032513656324322	0.0
38-39	0.1308141007670509	4.825130049317654E-5	9.650260098635308E-6	9.650260098635308E-6	0.20499323758023588	0.0
40-41	0.1322447518266736	4.825130049317654E-5	9.650260098635308E-6	9.650260098635308E-6	0.20680024878370534	0.0
42-43	0.13260663658037242	5.0663865517835366E-5	9.650260098635308E-6	9.650260098635308E-6	0.20842873017535005	0.0
44-45	0.1334462092089537	5.30764305424942E-5	1.4475390147952962E-5	9.650260098635308E-6	0.20989556971034262	0.0
46-47	0.13429543209763362	6.755182069044716E-5	1.4475390147952962E-5	9.650260098635308E-6	0.21153852649213528	0.0
48-49	0.13526528323754644	7.237695073976482E-5	1.4475390147952962E-5	9.650260098635308E-6	0.21268449487884822	0.0
50-51	0.1363943636690868	7.720208078908247E-5	1.4475390147952962E-5	9.650260098635308E-6	0.2140500066828051	0.0
52-53	0.1376706105671313	8.202721083840012E-5	1.4475390147952962E-5	9.650260098635308E-6	0.2153745048813428	0.0
54-55	0.13833406594891248	8.202721083840012E-5	1.4475390147952962E-5	9.650260098635308E-6	0.21630334241583646	0.0
56-57	0.13909402393168002	8.202721083840012E-5	1.4475390147952962E-5	9.650260098635308E-6	0.21736969615673568	2.412565024658827E-6
58-59	0.1392653160484308	8.926490591237661E-5	1.688795517261179E-5	9.650260098635308E-6	0.21835402268679646	4.825130049317654E-6
60-61	0.13958859976173507	9.891516601101191E-5	1.9300520197270616E-5	9.650260098635308E-6	0.21905366654394753	1.4475390147952962E-5
62-63	0.13987569499966948	1.0132773103567073E-4	1.9300520197270616E-5	9.650260098635308E-6	0.2199752663833672	1.4475390147952962E-5
64-65	0.1399432468203599	1.0856542610964722E-4	1.9300520197270616E-5	9.650260098635308E-6	0.2209813059986499	1.4475390147952962E-5
66-67	0.14000356094597638	1.1097799113430605E-4	1.9300520197270616E-5	9.650260098635308E-6	0.22177021476171338	1.4475390147952962E-5
68-69	0.14005422481149424	1.158031211836237E-4	1.9300520197270616E-5	9.650260098635308E-6	0.22256877378487544	1.4475390147952962E-5
70-71	0.14017002793267785	1.158031211836237E-4	1.9300520197270616E-5	9.650260098635308E-6	0.22331666894251967	1.4475390147952962E-5
72-73	0.14022310436322036	1.158031211836237E-4	1.9300520197270616E-5	9.650260098635308E-6	0.22387397146321586	1.4475390147952962E-5
74-75	0.14025205514351624	1.25453381282259E-4	1.9300520197270616E-5	9.650260098635308E-6	0.22458567814549021	1.4475390147952962E-5
76-77	0.14028824361888614	1.326910763562355E-4	1.9300520197270616E-5	9.650260098635308E-6	0.22540836281889887	1.4475390147952962E-5
78-79	0.14032925722430534	1.495790315288473E-4	1.9300520197270616E-5	9.650260098635308E-6	0.22646506629969945	1.4475390147952962E-5
80-81	0.14037750852479852	1.495790315288473E-4	1.9300520197270616E-5	1.4475390147952962E-5	0.22731670175340402	1.9300520197270616E-5
82-83	0.14040887187011908	1.519915965535061E-4	2.1713085221929442E-5	1.4475390147952962E-5	0.2282021131174538	1.9300520197270616E-5
84-85	0.14047642369080954	1.6164185665214141E-4	2.412565024658827E-5	1.4475390147952962E-5	0.22904892344110905	1.9300520197270616E-5
86-87	0.140529500121352	1.688795517261179E-4	2.412565024658827E-5	1.4475390147952962E-5	0.22991985941501086	1.9300520197270616E-5
88-89	0.14057533885682055	1.7370468177543556E-4	2.412565024658827E-5	1.4475390147952962E-5	0.230802858214036	1.9300520197270616E-5
90-91	0.1406115273321904	1.785298118247532E-4	2.65382152712471E-5	1.4475390147952962E-5	0.23138187381995412	1.9300520197270616E-5
92-93	0.1406187650272644	1.785298118247532E-4	2.8950780295905925E-5	1.4475390147952962E-5	0.23188127478005852	1.9300520197270616E-5
94-95	0.14068149171790553	1.8335494187407086E-4	2.8950780295905925E-5	1.4475390147952962E-5	0.23257126837711095	1.9300520197270616E-5
96-97	0.14070561736815212	1.9059263694804735E-4	2.8950780295905925E-5	1.688795517261179E-5	0.23328780018943462	2.1713085221929442E-5
98-99	0.14073215558342336	1.9300520197270616E-4	3.377591034522358E-5	1.9300520197270616E-5	0.23384751527515546	3.377591034522358E-5
100-101	0.14078523201396587	1.9300520197270616E-4	3.377591034522358E-5	1.9300520197270616E-5	0.23437104188550642	3.377591034522358E-5
102-103	0.14081900792431107	1.9300520197270616E-4	3.377591034522358E-5	2.412565024658827E-5	0.23482701667516692	3.377591034522358E-5
104-105	0.14085760896470562	1.9300520197270616E-4	3.377591034522358E-5	2.65382152712471E-5	0.23536743124069054	3.377591034522358E-5
106-107	0.14086725922480425	1.9783033202202383E-4	3.377591034522358E-5	2.8950780295905925E-5	0.23592955889143602	3.377591034522358E-5
108-109	0.14090344770017416	2.147182871946356E-4	3.377591034522358E-5	2.8950780295905925E-5	0.2365230498875021	3.377591034522358E-5
110-111	0.14096617439081527	2.1713085221929443E-4	3.618847536988241E-5	2.8950780295905925E-5	0.2370514016279024	3.377591034522358E-5
112-113	0.14100236286618517	2.1713085221929443E-4	4.342617044385889E-5	2.8950780295905925E-5	0.23756286541313004	3.377591034522358E-5
114-115	0.1410168382563331	2.1954341724395325E-4	4.342617044385889E-5	2.8950780295905925E-5	0.2381346433239742	3.377591034522358E-5
116-117	0.14104096390657972	2.2678111231792976E-4	4.342617044385889E-5	2.8950780295905925E-5	0.23857373015846212	3.377591034522358E-5
118-119	0.14106026442677694	2.2678111231792976E-4	4.342617044385889E-5	2.8950780295905925E-5	0.23913827037423227	3.860104039454123E-5
120-121	0.14109886546717149	2.2678111231792976E-4	4.342617044385889E-5	2.8950780295905925E-5	0.23974141163039697	4.1013605419200065E-5
122-123	0.14112057855239343	2.2678111231792976E-4	4.342617044385889E-5	2.8950780295905925E-5	0.24025770054567397	4.342617044385889E-5
124-125	0.1411350539425414	2.2678111231792976E-4	4.5838735468517716E-5	2.8950780295905925E-5	0.24079811511119753	4.342617044385889E-5
126-127	0.14115917959278798	2.2678111231792976E-4	5.30764305424942E-5	3.136334532056475E-5	0.24136506789199236	4.342617044385889E-5
128-129	0.14118330524303457	2.2678111231792976E-4	5.30764305424942E-5	3.377591034522358E-5	0.24204299866392148	4.342617044385889E-5
130-131	0.14119536806815786	2.2919367734258858E-4	5.30764305424942E-5	3.377591034522358E-5	0.2427354048259986	4.342617044385889E-5
132-133	0.14120260576323185	2.3401880739190622E-4	5.30764305424942E-5	3.377591034522358E-5	0.24329753247674407	4.342617044385889E-5
134-135	0.14122431884845377	2.4366906749054154E-4	5.30764305424942E-5	3.618847536988241E-5	0.243835534477243	4.825130049317654E-5
136-137	0.1412460319336757	2.50906762564518E-4	5.5488995567153024E-5	3.860104039454123E-5	0.24439042443291453	4.825130049317654E-5
138-139	0.1412460319336757	2.50906762564518E-4	5.790156059181185E-5	3.860104039454123E-5	0.24501769133932583	4.825130049317654E-5
>>END_MODULE
