##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.2d.S.3.R1.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24153852
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	72-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.463143518474816	36.0	34.0	38.0	31.0	39.0
2	34.77277880977328	35.0	34.0	38.0	31.0	39.0
3	34.89844576343351	35.0	34.0	38.0	31.0	39.0
4	34.90137461304309	36.0	34.0	38.0	31.0	39.0
5	34.758764606158884	35.0	34.0	37.0	31.0	39.0
6	34.7378091080462	35.0	34.0	37.0	31.0	39.0
7	34.7747411882792	35.0	34.0	37.0	32.0	39.0
8	34.88951281973575	35.0	34.0	37.0	32.0	39.0
9	34.857604534465146	35.0	34.0	37.0	32.0	39.0
10-14	34.83574952765298	35.0	34.0	37.4	31.2	39.0
15-19	34.851826938411314	35.0	34.0	37.0	31.0	39.0
20-24	34.839054582267046	35.0	34.0	37.0	31.0	39.0
25-29	34.842910878148956	35.0	34.0	37.0	31.0	39.0
30-34	34.81828215226291	35.0	34.0	37.0	31.0	39.0
35-39	34.808720348207814	35.0	34.0	37.0	31.0	39.0
40-44	34.762116924455775	35.0	34.0	37.0	31.0	39.0
45-49	34.65324324252711	35.0	34.0	37.0	31.0	39.0
50-54	34.62848450011204	35.0	34.0	37.0	31.0	39.0
55-59	34.6197558799317	35.0	34.0	37.0	31.0	39.0
60-64	34.58880610844184	35.0	34.0	37.0	30.2	39.0
65-69	34.55770087520616	35.0	34.0	37.0	30.0	39.0
70-74	34.557370056086306	35.0	34.0	37.0	30.0	39.0
75-79	34.53921750775045	35.0	34.0	37.0	30.0	39.0
80-84	34.521419901313834	35.0	34.0	37.0	30.0	39.0
85-89	34.50526051384511	35.0	34.0	37.0	30.0	39.0
90-94	34.48086037250973	35.0	34.0	37.0	30.0	39.0
95-99	34.45926759986445	35.0	34.0	37.0	30.0	39.0
100-104	34.4200353542855	35.0	34.0	37.0	30.0	39.0
105-109	34.390714455757106	35.0	34.0	37.0	30.0	39.0
110-114	34.36210958665498	35.0	34.0	37.0	30.0	39.0
115-119	34.32328995500897	35.0	34.0	37.0	30.0	39.0
120-124	34.281690598176645	35.0	34.0	37.0	30.0	39.0
125-129	34.26227272369071	35.0	34.0	37.0	30.0	39.0
130-134	34.21572002861001	35.0	34.0	37.0	29.0	39.0
135-139	34.175778836847975	35.0	34.0	37.0	29.0	39.0
140-144	34.13610650855896	35.0	34.0	37.0	29.0	39.0
145-149	34.16742677953539	35.0	34.0	37.0	29.2	39.0
150	35.04977936441172	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	11.0
22	199.0
23	2597.0
24	18579.0
25	79029.0
26	204685.0
27	376390.0
28	560728.0
29	734639.0
30	924636.0
31	1165364.0
32	1490306.0
33	1955334.0
34	2687896.0
35	4169525.0
36	8294080.0
37	1489849.0
38	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.63226056034458	10.495265931082132	14.726305352868769	38.14616815570452
2	23.02447487739045	11.930679865801656	36.42331451563422	28.621530741173686
3	21.813330251020545	16.189065727556272	27.914182740463517	34.08342128095966
4	27.216384982502774	23.848727892994173	21.911076196006224	27.023810928496832
5	27.153336462443576	29.61170010670357	24.025451697496766	19.209511733356088
6	20.278649838801943	35.709285323605236	25.560330460262055	18.451734377330766
7	17.01921595632461	24.382304042592327	39.51937980195524	19.079100199127815
8	19.297469448420696	24.074000701752208	31.219109188710277	25.409420661116823
9	20.136906629398876	22.98498863575861	32.298669436910096	24.579435297932413
10-14	21.133515398268724	28.49979157316688	26.688903830361298	23.677789198203094
15-19	21.388967822903744	27.480267982135075	27.17712465586973	23.953639539091448
20-24	21.317682148527926	27.486361107978635	27.13111535578757	24.06484138770587
25-29	21.25696312677208	27.663055075605136	26.80876849683438	24.271213300788403
30-34	21.29724499369693	27.428692386857488	27.01920619352794	24.25485642591764
35-39	21.5781913672234	27.18744853039511	26.925098294809587	24.309261807571907
40-44	21.77845493869988	27.25758272429838	26.74110468644543	24.222857650556314
45-49	22.05643431882798	27.166887676692546	26.652124086347932	24.124553918131543
50-54	22.015925576213675	27.419428065603952	26.562533599749095	24.002112758433277
55-59	21.91863495260016	27.35477799984253	26.651331394995047	24.075255652562262
60-64	22.16618524370091	27.119385923524025	26.438284334774416	24.276144498000654
65-69	21.99526050947141	27.272301355264172	26.633121407272753	24.099316727991663
70-74	22.370405909283246	27.00615238034248	26.554056338401132	24.06938537197314
75-79	22.250162599582204	26.866291623613424	26.54980661107694	24.333739165727437
80-84	22.48164382716376	26.84497791888678	26.60881475697991	24.06456349696955
85-89	22.36130052002922	26.802616420166565	26.609720035563438	24.226363024240776
90-94	22.3574851050081	26.53555579513182	26.621564778579366	24.485394321280715
95-99	22.585879407725184	26.588486643192784	26.79400044375798	24.031633505324052
100-104	22.51850706468224	26.407718601036436	26.870275774403733	24.20349855987759
105-109	22.640767951434977	26.36472930300154	26.71976285455236	24.274739891011127
110-114	22.598463900845132	26.41206796507436	26.698264869989043	24.291203264091465
115-119	22.73802073779776	26.58533320853055	26.618521210213398	24.05812484345829
120-124	22.701789268413844	26.45836534958166	26.565356996028903	24.274488385975587
125-129	23.10636310844502	26.41485278034157	26.440373601974237	24.03841050923918
130-134	22.82316938158899	26.67627610932807	26.3454810323131	24.15507347676984
135-139	22.921813694443898	26.519945513827842	26.388180388157995	24.17006040357026
140-144	22.886074503560476	26.321214178486603	26.67894708463131	24.113764233321614
145-149	22.337265196305008	26.287815818931428	27.08756301333891	24.287355971424653
150	22.185371428217433	24.909150721751725	27.750009861800866	25.155467988229972
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	3.5
12	8.5
13	11.5
14	18.5
15	38.5
16	89.5
17	222.5
18	514.5
19	899.5
20	1502.5
21	2439.0
22	4005.5
23	6611.0
24	10743.5
25	16501.0
26	23430.0
27	33577.5
28	51711.5
29	76403.5
30	103909.5
31	131590.5
32	158911.0
33	190417.0
34	225264.0
35	271992.5
36	337501.5
37	404727.5
38	471578.5
39	571651.5
40	709310.5
41	860523.5
42	1019254.0
43	1178562.0
44	1326270.0
45	1447780.0
46	1537710.0
47	1570631.5
48	1532435.5
49	1489985.0
50	1411258.5
51	1259401.0
52	1076489.0
53	916078.5
54	769888.5
55	636911.0
56	545436.5
57	454250.0
58	376538.0
59	308313.5
60	233479.5
61	159012.0
62	92724.5
63	53996.5
64	35371.5
65	22709.5
66	15022.0
67	7336.0
68	3120.5
69	2102.0
70	2370.5
71	1851.0
72	873.5
73	295.5
74	139.0
75	75.5
76	37.5
77	26.5
78	11.0
79	2.0
80	0.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0014490442352631788
3	0.1384292658578847
4	1.1178341243458807E-4
5	8.694265411579072E-5
6	1.0350315966165563E-4
7	7.866240134285828E-5
8	1.1178341243458807E-4
9	7.452227495639205E-5
10-14	2.1611459737353697E-4
15-19	2.5999993707007894E-4
20-24	3.883438550505319E-4
25-29	3.4694259118586967E-4
30-34	2.922929228845155E-4
35-39	1.382802213079719E-4
40-44	2.7324834150677086E-4
45-49	1.896177885001531E-4
50-54	1.0681526077082861E-4
55-59	1.4821652463549087E-4
60-64	1.6394900490406252E-4
65-69	0.05569049607491177
70-74	1.8796174417104962E-4
75-79	1.3993629851284065E-4
80-84	1.150955840672802E-4
85-89	8.694277865990657E-5
90-94	1.3993663222158206E-4
95-99	1.0019156212663894E-4
100-104	6.87266505862313E-5
105-109	8.694367207642339E-5
110-114	5.87907609905084E-5
115-119	5.5478914149800176E-5
120-124	9.522577858211266E-5
125-129	9.688297333414572E-5
130-134	3.891958567699626E-5
135-139	4.140586972590457E-5
140-144	4.8046726999383566E-5
145-149	4.472073646716341E-5
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
72-73	2.0
74-75	1.0
76-77	4.0
78-79	2.0
80-81	5.0
82-83	9.0
84-85	6.0
86-87	10.0
88-89	8.0
90-91	14.0
92-93	20.0
94-95	28.0
96-97	29.0
98-99	25.0
100-101	32.0
102-103	26.0
104-105	37.0
106-107	47.0
108-109	40.0
110-111	58.0
112-113	46.0
114-115	56.0
116-117	53.0
118-119	71.0
120-121	94.0
122-123	107.0
124-125	100.0
126-127	138.0
128-129	175.0
130-131	234.0
132-133	284.0
134-135	446.0
136-137	791.0
138-139	1999.0
140-141	5170.0
142-143	7760.0
144-145	67854.0
146-147	757106.0
148-149	1357571.0
150-151	2.1953394E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.516180659268656
#Duplication Level	Percentage of total
1	34.47667507673469
2	8.374623913781553
3	4.918749230311579
4	3.25580884141995
5	2.5606585970223117
6	2.0427509310857883
7	1.6731944572523374
8	1.451273230669915
9	1.2452281296058794
>10	16.00361285865776
>50	5.272494399163515
>100	10.472384791797703
>500	3.5676933682967413
>1k	4.024383333612167
>5k	0.5437800908993379
>10k+	0.11668874968882202
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	4.140126386466225E-6	0.0	0.0	2.93948973439102E-4	2.0700631932331125E-5
2	8.28025277293245E-6	2.8980884705263574E-5	0.0	1.2420379159398675E-5	0.002318470776421086	2.484075831879735E-5
3	1.2420379159398675E-5	2.8980884705263574E-5	0.0	1.2420379159398675E-5	0.004289170936379009	5.796176941052715E-5
4	5.796176941052715E-5	2.8980884705263574E-5	0.0	1.2420379159398675E-5	0.006255730969950466	8.28025277293245E-5
5	6.210189579699338E-5	2.8980884705263574E-5	0.0	1.2420379159398675E-5	0.007361144715136948	8.694265411579073E-5
6	8.694265411579073E-5	2.8980884705263574E-5	0.0	1.2420379159398675E-5	0.009050316280815167	9.108278050225695E-5
7	1.0350315966165563E-4	3.7261137478196024E-5	0.0	1.2420379159398675E-5	0.010706366835401658	9.522290688872318E-5
8	2.0700631932331126E-4	4.5541390251128474E-5	0.0	1.2420379159398675E-5	0.014039168576506968	9.93630332751894E-5
9	2.7738846789323706E-4	4.5541390251128474E-5	0.0	1.2420379159398675E-5	0.01729744804265589	9.93630332751894E-5
10-11	3.7882156436165956E-4	5.1751579830827815E-5	0.0	2.0700631932331125E-5	0.02984824118322825	1.2834391798045297E-4
12-13	4.3471327057895365E-4	5.3821643024060924E-5	0.0	2.0700631932331125E-5	0.03905588226672914	1.614649290721828E-4
14-15	4.7818459763684896E-4	6.210189579699338E-5	0.0	2.2770695125564237E-5	0.04627005249514653	1.7388530823158146E-4
16-17	5.34076303854143E-4	7.038214856992583E-5	0.0	2.484075831879735E-5	0.050764159687655616	1.7802543461804766E-4
18-19	6.230890211631668E-4	7.659233814962516E-5	0.0	2.484075831879735E-5	0.05404106972254363	1.821655610045139E-4
20-21	0.0010929933660270834	1.0557322285488873E-4	0.0	2.484075831879735E-5	0.05666797991475646	1.821655610045139E-4
22-23	0.0016167193539150609	1.0971334924135496E-4	0.0	2.484075831879735E-5	0.05927211941184371	1.8630568739098013E-4
24-25	0.0017988849149195747	1.159235388210543E-4	0.0	2.484075831879735E-5	0.06206877478590164	1.8837575058421323E-4
26-27	0.0018527065579436357	1.2006366520752053E-4	0.0	2.484075831879735E-5	0.06449488884837085	1.9251587697067946E-4
28-29	0.0019313689592864937	1.2420379159398676E-4	0.0	3.105094789849668E-5	0.06724393276898442	1.9458594016391256E-4
30-31	0.002136305215416572	1.2420379159398676E-4	0.0	4.3471327057895365E-5	0.07040905939143785	2.007961297436119E-4
32-33	0.0022149676167594304	1.2420379159398676E-4	0.0	6.0031832603760264E-5	0.07333612874666948	2.318470776421086E-4
34-35	0.00230812046045492	1.2420379159398676E-4	0.0	9.522290688872318E-5	0.07608517266728304	2.4219739360827416E-4
36-37	0.0023453815979331163	1.2834391798045297E-4	0.0	1.0350315966165563E-4	0.07856924849916277	2.504776463812066E-4
38-39	0.002353661850706049	1.2834391798045297E-4	0.0	1.138534756278212E-4	0.0812458402080132	2.5461777276767283E-4
40-41	0.002380572672218079	1.345541075601523E-4	0.0	1.159235388210543E-4	0.08372991603989294	2.6289802554060524E-4
42-43	0.0023867828617977786	1.3869423394661856E-4	0.0	1.159235388210543E-4	0.08611462883849748	2.7738846789323706E-4
44-45	0.002415763746503042	1.4697448671955097E-4	0.0	1.159235388210543E-4	0.08870220783003888	2.815285942797033E-4
46-47	0.0024385344416286065	1.5318467629925033E-4	0.0	1.159235388210543E-4	0.0928609647852442	2.815285942797033E-4
48-49	0.0024550949471744713	1.5732480268571653E-4	0.0	1.159235388210543E-4	0.09542784314485325	2.856687206661695E-4
50-51	0.0024902860214594345	1.6146492907218276E-4	0.0	1.159235388210543E-4	0.09837147300563073	3.001591630188013E-4
52-53	0.00252754715893763	1.65605055458649E-4	0.0	1.159235388210543E-4	0.10074997561465558	3.0636935259850066E-4
54-55	0.0025710184859955255	1.65605055458649E-4	0.0	1.159235388210543E-4	0.1024888286969714	3.0636935259850066E-4
56-57	0.0026289802554060526	1.6974518184511523E-4	0.0	1.159235388210543E-4	0.10446780910970226	3.0636935259850066E-4
58-59	0.002689012088009813	1.8009549781128077E-4	0.0	1.159235388210543E-4	0.10649026084949101	3.0636935259850066E-4
60-61	0.0028401267011158304	1.9044581377744636E-4	0.0	1.2006366520752053E-4	0.10824774450054592	3.1050947898496686E-4
62-63	0.0029208591656519215	1.9458594016391256E-4	0.0	1.2006366520752053E-4	0.11041096053747451	3.125795421782E-4
64-65	0.0029353496080045532	2.007961297436119E-4	0.0	1.2006366520752053E-4	0.1131227433206099	3.146496053714331E-4
66-67	0.0029519101135504185	2.2563688806240926E-4	0.0	1.2006366520752053E-4	0.11536876188526782	3.208597949511324E-4
68-69	0.0029746808086759825	2.3805726722180796E-4	0.0	1.2213372840075363E-4	0.1172545894543032	3.31210110917298E-4
70-71	0.0030264323885068104	2.525477095744397E-4	0.0	1.2834391798045297E-4	0.11916318771846413	3.3742030049699735E-4
72-73	0.003038852767666209	2.608279623473722E-4	0.0	1.2834391798045297E-4	0.12074678606128746	3.3949036369023046E-4
74-75	0.0030657635891782396	2.608279623473722E-4	0.0	1.2834391798045297E-4	0.12225379206596115	3.3949036369023046E-4
76-77	0.003094744473883503	2.608279623473722E-4	0.0	1.366241707533854E-4	0.12359105288878974	3.3949036369023046E-4
78-79	0.003142355927327865	2.608279623473722E-4	0.0	1.5318467629925033E-4	0.1249324538380048	3.3949036369023046E-4
80-81	0.0031568463696804968	2.670381519270715E-4	0.0	1.5318467629925033E-4	0.12629248535595897	3.3949036369023046E-4
82-83	0.0031816871279992938	2.7324834150677086E-4	0.0	1.5318467629925033E-4	0.12777258053912063	3.3949036369023046E-4
84-85	0.003237578834216588	2.7531840470000396E-4	0.0	1.5732480268571653E-4	0.12945347185202594	3.3949036369023046E-4
86-87	0.003281050161274483	2.8773878385940263E-4	0.0	1.635349922654159E-4	0.1308590447602312	3.3949036369023046E-4
88-89	0.003295540603627115	2.8980884705263573E-4	0.0	1.676751186518821E-4	0.13228531830036883	3.3949036369023046E-4
90-91	0.0033038208564000475	2.93948973439102E-4	0.0	1.6974518184511523E-4	0.1340531522673899	3.3949036369023046E-4
92-93	0.0033183112987526793	2.93948973439102E-4	0.0	1.6974518184511523E-4	0.13587480787743506	3.3949036369023046E-4
94-95	0.003380413194549673	2.93948973439102E-4	0.0	1.7388530823158146E-4	0.13731764192311852	3.3949036369023046E-4
96-97	0.0034632157222789974	2.960190366323351E-4	0.0	1.7802543461804766E-4	0.13853897920712605	3.3949036369023046E-4
98-99	0.0035418781236218554	3.0222922621203445E-4	0.0	1.8630568739098013E-4	0.1396278324467667	3.3949036369023046E-4
100-101	0.0036702220416023083	3.125795421782E-4	0.0	1.9044581377744636E-4	0.14071047549682758	3.3949036369023046E-4
102-103	0.003707483179080504	3.146496053714331E-4	0.0	1.9251587697067946E-4	0.1417248064615118	3.3949036369023046E-4
104-105	0.0037488844429451667	3.146496053714331E-4	0.0	2.007961297436119E-4	0.14271636673107047	3.3949036369023046E-4
106-107	0.0038130564019353934	3.208597949511324E-4	0.0	2.132165089030106E-4	0.14387146199289455	3.3949036369023046E-4
108-109	0.0038399672234474234	3.2499992133759863E-4	0.0	2.1942669848270993E-4	0.14493547447421637	3.3949036369023046E-4
110-111	0.0038834385505053188	3.332801741105311E-4	0.0	2.1942669848270993E-4	0.14595187550209382	3.3949036369023046E-4
112-113	0.003986941710166975	3.394903636902304E-4	0.0	2.1942669848270993E-4	0.14696413640358483	3.3949036369023046E-4
114-115	0.004049043605963968	3.477706164631629E-4	0.0	2.2149676167594303E-4	0.14819789406675177	3.3949036369023046E-4
116-117	0.004131846133693292	3.6019099562256153E-4	0.0	2.2770695125564236E-4	0.14904869003917057	3.3949036369023046E-4
118-119	0.0041711773343647215	3.788215643616596E-4	0.0	2.318470776421086E-4	0.14986843506369085	3.4570055326992976E-4
120-121	0.004274680494026377	3.850317539413589E-4	0.0	2.4840758318797347E-4	0.15072337116249618	3.477706164631629E-4
122-123	0.004512737761248185	3.850317539413589E-4	0.0	2.649680887338384E-4	0.15147480410163977	3.477706164631629E-4
124-125	0.004647291868808337	3.850317539413589E-4	0.0	2.649680887338384E-4	0.15215585489221345	3.477706164631629E-4
126-127	0.004684553006286533	3.933120067142914E-4	0.0	2.649680887338384E-4	0.15290935789455032	3.477706164631629E-4
128-129	0.0047363045861173615	3.933120067142914E-4	2.0700631932331125E-6	2.7117827831353775E-4	0.1535697080531917	3.477706164631629E-4
130-131	0.004899839578382777	3.933120067142914E-4	4.140126386466225E-6	2.7738846789323706E-4	0.15423626840141275	3.477706164631629E-4
132-133	0.004997132548464733	3.933120067142914E-4	4.140126386466225E-6	2.8980884705263573E-4	0.1549028287496338	3.477706164631629E-4
134-135	0.005102705771319622	3.933120067142914E-4	4.140126386466225E-6	2.93948973439102E-4	0.15568531263667593	3.477706164631629E-4
136-137	0.00524761019484594	4.0573238587369005E-4	4.140126386466225E-6	2.960190366323351E-4	0.15632910228977143	3.477706164631629E-4
138-139	0.005257960510812106	4.098725122601563E-4	4.140126386466225E-6	3.001591630188013E-4	0.15699773270118572	3.477706164631629E-4
>>END_MODULE
