##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Col0.control.2d.S.3.R2.clean.fq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24153852
Total Bases	3.6 Gbp
Sequences flagged as poor quality	0
Sequence length	56-150
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.897734282714	37.0	35.0	38.0	33.0	39.0
2	35.259853583602315	35.0	34.0	37.0	33.0	39.0
3	35.39103634484471	35.0	34.0	37.0	33.0	39.0
4	35.453980507953766	36.0	35.0	38.0	33.0	39.0
5	35.37285448300337	36.0	34.0	37.0	33.0	39.0
6	35.32615042105913	35.0	34.0	37.0	33.0	39.0
7	35.38300267799935	35.0	34.0	37.0	33.0	39.0
8	35.47706374122024	36.0	34.0	38.0	33.0	39.0
9	35.4704616472768	36.0	34.0	38.0	33.0	39.0
10-14	35.43384806696671	35.8	34.4	37.8	33.0	39.0
15-19	35.40720540972099	36.0	34.0	38.0	33.0	39.0
20-24	35.37893460637251	36.0	34.0	38.0	33.0	39.0
25-29	35.370106995770286	36.0	34.0	38.0	33.0	39.0
30-34	35.35331925524757	36.0	34.0	38.0	32.6	39.0
35-39	35.32526461617799	36.0	34.0	38.0	32.0	39.0
40-44	35.306588489488135	36.0	34.0	38.0	32.0	39.0
45-49	35.27867595611665	36.0	34.0	38.0	32.0	39.0
50-54	35.257438250428955	36.0	34.0	38.0	32.0	39.0
55-59	35.22614730497414	36.0	34.0	38.0	32.0	39.0
60-64	35.18383914158215	36.0	34.0	38.0	32.0	39.0
65-69	35.14625613897617	36.0	34.0	38.0	32.0	39.0
70-74	35.1274569884355	36.0	34.0	38.0	32.0	39.0
75-79	35.06952440896863	35.4	34.0	37.6	32.0	39.0
80-84	34.99441564600923	35.4	34.0	37.2	31.6	39.0
85-89	34.9750187869491	35.0	34.0	37.0	31.2	39.0
90-94	34.9264872213491	35.0	34.0	37.0	31.0	39.0
95-99	34.8597043011311	35.0	34.0	37.0	31.0	39.0
100-104	34.7937413901375	35.0	34.0	37.0	31.0	39.0
105-109	34.737638658379254	35.0	34.0	37.0	30.8	39.0
110-114	34.66363318304872	35.0	34.0	37.0	30.0	39.0
115-119	34.5983429591962	35.0	34.0	37.0	30.0	39.0
120-124	34.52494838422723	35.0	34.0	37.0	30.0	39.0
125-129	34.442419730951734	35.0	34.0	37.0	30.0	39.0
130-134	34.36364590710548	35.0	34.0	37.0	30.0	39.0
135-139	34.277098907757804	35.0	34.0	37.0	29.0	39.0
140-144	34.18166074922303	35.0	34.0	37.0	29.0	39.0
145-149	34.144161454295514	35.0	34.0	37.0	29.0	39.0
150	34.97071731393374	35.0	34.0	37.0	31.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	170.0
22	1544.0
23	8511.0
24	30558.0
25	76274.0
26	153130.0
27	265320.0
28	392962.0
29	524342.0
30	680205.0
31	889662.0
32	1189973.0
33	1649025.0
34	2447172.0
35	4092357.0
36	9765672.0
37	1986959.0
38	8.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.61961893283109	19.047442205077683	14.726487518429773	32.606451343661455
2	23.99016107244509	26.12789877159138	32.28303709072988	17.598903065233653
3	19.65573441453562	29.4874333087741	29.442566759123967	21.414265517566307
4	23.22953291259713	34.22022706771574	21.452180795013565	21.098059224673563
5	25.178621612817697	35.079319853413025	21.571482676965974	18.170575856803296
6	19.16462848244661	38.89846224113653	21.872515406652322	20.06439386976454
7	20.566835467899697	20.926405444564285	36.16548615102883	22.34127293650719
8	21.55531134330044	23.57225671499519	27.954448011025324	26.91798393067905
9	23.422230955128814	23.754289791955337	27.474830929658754	25.348648323257095
10-14	23.067423142838027	28.767822447266983	24.99954727717589	23.165207132719097
15-19	23.12946936993735	27.697169793041702	26.470951300024524	22.702409536996417
20-24	23.058696995978543	27.618817314273187	26.413056809713193	22.909428880035076
25-29	23.141725800091844	27.230956784863963	26.40227653957638	23.225040875467815
30-34	22.995118956595412	26.96984232577065	26.59217502864554	23.442863688988407
35-39	23.30624614243724	26.694097488052837	26.659967942173363	23.339688427336558
40-44	23.526458636908103	26.330379932774285	26.667815137726276	23.475346292591343
45-49	23.424983311150534	26.159861375320176	26.739109770151774	23.676045543377512
50-54	23.649505044988313	26.193473622289925	26.442989933390248	23.714031399331514
55-59	23.687837211750008	26.22082787178197	26.4524563565047	23.63887855996332
60-64	23.389419131462052	26.19237343305938	26.52747610745364	23.89073132802493
65-69	23.626482839285213	26.101756323863956	26.533964057950815	23.73779677890002
70-74	23.755942626071185	26.075138075964375	26.239254411279617	23.92966488668482
75-79	23.572448499037932	26.197468501170114	26.51498705683504	23.71509594295692
80-84	23.921257820615114	26.212783000346896	26.177079266919463	23.688879912118527
85-89	23.646590874332524	26.31011150681129	26.420549829912147	23.622747788944036
90-94	23.66169213583494	26.07824004686925	26.458307476314957	23.801760340980852
95-99	23.654665281250757	26.25580117380007	26.47268967082516	23.616843874124005
100-104	23.88610933203445	26.06401216404029	26.4295118446511	23.62036665927416
105-109	23.701188839090147	26.230968773721653	26.338409486281844	23.72943290090636
110-114	23.773342557750706	26.363408977133034	26.229835553683323	23.63341291143294
115-119	23.930564431052737	26.430982134117226	26.375785790821972	23.262667644008065
120-124	23.90522172017	26.57886883445839	26.50847077938312	23.007438665988488
125-129	23.833822211628167	26.65974352240676	26.361935584399475	23.144498681565594
130-134	23.986568001523352	26.678029412726588	26.377596037259497	22.957806548490563
135-139	23.775681629520946	26.650681551873046	26.685102556313883	22.88853426229213
140-144	23.7881122116995	26.79375982504179	26.48555170589266	22.93257625736605
145-149	23.575422834727963	26.92679779108763	26.43910810530302	23.058671268881383
150	23.715154450543537	25.855030198091676	26.714818916191536	23.714996435173248
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	8.5
3	21.0
4	28.0
5	29.0
6	21.5
7	17.5
8	18.5
9	12.0
10	9.5
11	8.0
12	6.0
13	8.0
14	14.0
15	28.0
16	57.5
17	108.5
18	181.0
19	282.5
20	454.5
21	673.5
22	1020.5
23	1661.5
24	2729.5
25	4315.0
26	6563.5
27	10034.5
28	15196.0
29	21526.5
30	29982.5
31	42808.5
32	61105.0
33	84342.5
34	115696.0
35	165423.0
36	236830.0
37	325096.0
38	435384.5
39	571053.0
40	734010.5
41	915179.0
42	1109361.0
43	1307811.5
44	1466282.0
45	1574717.0
46	1625375.5
47	1611885.0
48	1577222.0
49	1538074.5
50	1437773.5
51	1280569.0
52	1101523.0
53	931482.5
54	794482.5
55	680296.0
56	565242.5
57	453347.5
58	373951.5
59	306857.0
60	231893.0
61	158136.5
62	98863.5
63	59816.0
64	37118.5
65	19500.5
66	10744.5
67	6212.5
68	4012.0
69	2765.0
70	1839.5
71	1606.0
72	1014.5
73	752.5
74	652.5
75	407.0
76	264.5
77	85.0
78	16.5
79	1.5
80	1.5
81	0.5
82	0.5
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	8.28025277293245E-7
15-19	0.0
20-24	0.02884508855978748
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	8.28025277293245E-7
55-59	0.0
60-64	0.0
65-69	0.0
70-74	1.6560512265001052E-6
75-79	6.433762158071624E-4
80-84	0.055765961352453236
85-89	8.280286574425323E-7
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	3.041488253407718E-4
145-149	0.030272458885518525
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
56-57	1.0
58-59	0.0
60-61	0.0
62-63	1.0
64-65	1.0
66-67	1.0
68-69	4.0
70-71	1.0
72-73	4.0
74-75	4.0
76-77	9.0
78-79	9.0
80-81	15.0
82-83	14.0
84-85	20.0
86-87	26.0
88-89	33.0
90-91	48.0
92-93	44.0
94-95	63.0
96-97	70.0
98-99	94.0
100-101	94.0
102-103	103.0
104-105	91.0
106-107	112.0
108-109	140.0
110-111	139.0
112-113	146.0
114-115	154.0
116-117	160.0
118-119	215.0
120-121	218.0
122-123	277.0
124-125	280.0
126-127	366.0
128-129	415.0
130-131	529.0
132-133	688.0
134-135	913.0
136-137	1519.0
138-139	4640.0
140-141	8845.0
142-143	10485.0
144-145	63320.0
146-147	794468.0
148-149	1115329.0
150-151	2.2149744E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.07638024210988
#Duplication Level	Percentage of total
1	24.758525473118866
2	8.139520181988237
3	5.143296228168685
4	3.671917885145378
5	2.773815777730151
6	2.2203141957903996
7	1.7996605002257673
8	1.6094224768592527
9	1.3323603951106893
>10	17.422844971220933
>50	6.162644089455914
>100	12.941731088328304
>500	4.170758477723647
>1k	5.931472865917684
>5k	1.1952377989491048
>10k+	0.7264775942673541
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGAACAATGGCTTCCTCTATGCTCTCTTCCGCTACTATGGTTGCCTCTCC	32135	0.13304296142909214	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	2.691082151203046E-4	0.0	0.0	0.0	0.0025296172221308632	0.0
2	0.0014449041088767124	4.140126386466225E-6	0.0	0.0	0.010652545192377598	0.0
3	0.0021487255945759707	1.2420379159398675E-5	0.0	0.0	0.01475127031497916	0.0
4	0.004930890526281274	1.2420379159398675E-5	4.140126386466225E-6	0.0	0.021586618979034897	0.0
5	0.0050592344442617265	1.2420379159398675E-5	4.140126386466225E-6	0.0	0.02552801929895074	0.0
6	0.006057004903400087	1.2420379159398675E-5	4.140126386466225E-6	0.0	0.035364959593194495	0.0
7	0.00831337378402418	1.2420379159398675E-5	4.140126386466225E-6	0.0	0.04669648551295255	0.0
8	0.01528948674521977	1.2420379159398675E-5	4.140126386466225E-6	0.0	0.06222609958858736	0.0
9	0.01580286241714158	1.2420379159398675E-5	4.140126386466225E-6	0.0	0.07332991855708977	0.0
10-11	0.016457002386203245	1.65605055458649E-5	4.140126386466225E-6	0.0	0.12607926884705595	0.0
12-13	0.017189804756607766	1.65605055458649E-5	4.140126386466225E-6	0.0	0.1483697093117901	0.0
14-15	0.019969899625119837	1.65605055458649E-5	4.140126386466225E-6	0.0	0.15994550268834967	0.0
16-17	0.023188847890597327	1.8630568739098012E-5	8.28025277293245E-6	6.210189579699337E-6	0.16775378105322497	0.0
18-19	0.02914441969752899	2.6910821512030462E-5	8.28025277293245E-6	8.28025277293245E-6	0.17515011684264686	0.0
20-21	0.034441711409012525	2.8980884705263574E-5	8.28025277293245E-6	8.28025277293245E-6	0.18004167616825673	0.0
22-23	0.036621487951486995	3.31210110917298E-5	8.28025277293245E-6	8.28025277293245E-6	0.18522304434091919	0.0
24-25	0.041738684165159246	3.5191074284962915E-5	8.28025277293245E-6	8.28025277293245E-6	0.18999039987493505	0.0
26-27	0.04605683598624352	3.7261137478196024E-5	8.28025277293245E-6	8.28025277293245E-6	0.19345775572360052	0.0
28-29	0.0562332666441775	3.7261137478196024E-5	1.0350315966165562E-5	8.28025277293245E-6	0.19692718163545925	0.0
30-31	0.07847195552908083	4.140126386466225E-5	1.2420379159398675E-5	8.28025277293245E-6	0.20077956923806606	0.0
32-33	0.09843978509100743	4.96815166375947E-5	1.2420379159398675E-5	8.28025277293245E-6	0.2037335494148097	0.0
34-35	0.11269424023961064	5.3821643024060924E-5	1.2420379159398675E-5	8.28025277293245E-6	0.20661714744298343	0.0
36-37	0.11511207404930693	5.3821643024060924E-5	1.65605055458649E-5	8.28025277293245E-6	0.2087410322792406	0.0
38-39	0.1173932836882498	5.3821643024060924E-5	1.65605055458649E-5	8.28025277293245E-6	0.21032670068525716	0.0
40-41	0.11873882476385134	5.589170621729403E-5	1.65605055458649E-5	8.28025277293245E-6	0.2119599805447181	0.0
42-43	0.11897067184149343	6.210189579699338E-5	1.65605055458649E-5	8.28025277293245E-6	0.2138561584297196	0.0
44-45	0.11973866528618293	6.62420221834596E-5	1.65605055458649E-5	8.28025277293245E-6	0.21529071222263016	0.0
46-47	0.12088548029523408	7.866240134285827E-5	1.65605055458649E-5	8.28025277293245E-6	0.216932272334864	0.0
48-49	0.12203022524109197	8.694265411579073E-5	1.65605055458649E-5	8.28025277293245E-6	0.21815774974525803	0.0
50-51	0.12293070273014839	8.694265411579073E-5	1.65605055458649E-5	8.28025277293245E-6	0.21945774943060842	0.0
52-53	0.12389535217819501	8.901271730902383E-5	1.65605055458649E-5	8.28025277293245E-6	0.22062940519797836	2.0700631932331125E-6
54-55	0.12442321829246947	9.315284369549006E-5	1.65605055458649E-5	8.28025277293245E-6	0.22161475527795732	4.140126386466225E-6
56-57	0.12526159388572888	9.72929700819563E-5	1.8630568739098012E-5	8.28025277293245E-6	0.2226642773169265	4.140126386466225E-6
58-59	0.12543340913076723	1.0557322285488873E-4	2.0700631932331125E-5	8.28025277293245E-6	0.22365376752329194	4.140126386466225E-6
60-61	0.12570872753546722	1.138534756278212E-4	2.0700631932331125E-5	8.28025277293245E-6	0.2244031303992423	6.210189579699337E-6
62-63	0.12598611600336046	1.159235388210543E-4	2.0700631932331125E-5	8.28025277293245E-6	0.22536156965770926	8.28025277293245E-6
64-65	0.12602958733041836	1.28343917980453E-4	2.0700631932331125E-5	8.28025277293245E-6	0.2263220789793694	8.28025277293245E-6
66-67	0.12613723061646648	1.4283436033308476E-4	2.0700631932331125E-5	8.28025277293245E-6	0.22691825717902053	8.28025277293245E-6
68-69	0.1262076127650364	1.4490442352631786E-4	2.0700631932331125E-5	8.28025277293245E-6	0.22766762005497093	1.0350315966165562E-5
70-71	0.1263131859878913	1.5111461310601723E-4	2.0700631932331125E-5	8.28025277293245E-6	0.2284832249531048	1.2420379159398675E-5
72-73	0.12638770826284768	1.6146492907218276E-4	2.0700631932331125E-5	8.28025277293245E-6	0.22909182353191532	1.2420379159398675E-5
74-75	0.12640426876839356	1.65605055458649E-4	2.0700631932331125E-5	8.28025277293245E-6	0.22973147305862435	2.0700631932331125E-5
76-77	0.12642910952671235	1.7595537142481456E-4	2.0700631932331125E-5	8.28025277293245E-6	0.23049739644012063	2.0700631932331125E-5
78-79	0.12650363180166874	1.9251587697067946E-4	2.0700631932331125E-5	8.28025277293245E-6	0.23160695031169354	2.484075831879735E-5
80-81	0.12655952350788602	1.9458594016391256E-4	2.0700631932331125E-5	1.2420379159398675E-5	0.2324660265368853	2.8980884705263574E-5
82-83	0.1266092050245236	1.987260665503788E-4	2.0700631932331125E-5	1.65605055458649E-5	0.23322366966560862	2.8980884705263574E-5
84-85	0.12665888654116123	2.0700631932331126E-4	2.0700631932331125E-5	1.65605055458649E-5	0.23414898791298383	2.8980884705263574E-5
86-87	0.1267064979946056	2.0700631932331126E-4	2.0700631932331125E-5	1.65605055458649E-5	0.2349604526847312	2.8980884705263574E-5
88-89	0.1267437591320838	2.0700631932331126E-4	2.0700631932331125E-5	1.65605055458649E-5	0.23578226777244476	3.105094789849668E-5
90-91	0.1267582495744364	2.1114644570977746E-4	2.0700631932331125E-5	1.65605055458649E-5	0.23638258609848234	3.31210110917298E-5
92-93	0.12676859989040257	2.1114644570977746E-4	2.0700631932331125E-5	1.65605055458649E-5	0.23692494265510944	3.31210110917298E-5
94-95	0.12679965083830108	2.1321650890301057E-4	2.0700631932331125E-5	1.65605055458649E-5	0.2376618851519004	3.5191074284962915E-5
96-97	0.1268141412806537	2.1942669848270993E-4	2.0700631932331125E-5	1.65605055458649E-5	0.23829532448902974	3.7261137478196024E-5
98-99	0.12684105210216573	2.1942669848270993E-4	2.0700631932331125E-5	1.65605055458649E-5	0.23880870016095157	4.140126386466225E-5
100-101	0.12688245336603038	2.2149676167594303E-4	2.0700631932331125E-5	1.65605055458649E-5	0.23940901848698914	4.140126386466225E-5
102-103	0.1268948737451898	2.2356682486917616E-4	2.484075831879735E-5	1.65605055458649E-5	0.23996586548596888	4.140126386466225E-5
104-105	0.12691764444031536	2.2563688806240926E-4	2.484075831879735E-5	1.65605055458649E-5	0.2405309927377215	4.761145344436158E-5
106-107	0.12693627500905447	2.318470776421086E-4	2.484075831879735E-5	1.65605055458649E-5	0.2410547187256095	4.96815166375947E-5
108-109	0.12700458709443113	2.3598720402857483E-4	2.8980884705263574E-5	1.65605055458649E-5	0.24154532370240572	5.1751579830827815E-5
110-111	0.1270708291166146	2.3598720402857483E-4	3.31210110917298E-5	1.65605055458649E-5	0.2420814700694531	5.3821643024060924E-5
112-113	0.12709981000131987	2.3598720402857483E-4	3.31210110917298E-5	1.65605055458649E-5	0.2426817883954907	5.3821643024060924E-5
114-115	0.12711016031728603	2.3598720402857483E-4	3.31210110917298E-5	1.65605055458649E-5	0.24324484558405013	5.589170621729403E-5
116-117	0.12712258069644544	2.3598720402857483E-4	3.31210110917298E-5	1.65605055458649E-5	0.24372717030807342	6.210189579699337E-5
118-119	0.12713086094921838	2.3598720402857483E-4	3.7261137478196024E-5	1.65605055458649E-5	0.24426124661192755	6.62420221834596E-5
120-121	0.12713707113879807	2.3598720402857483E-4	3.7261137478196024E-5	1.65605055458649E-5	0.2449257368969554	6.831208537669271E-5
122-123	0.12715363164434396	2.4012733041504106E-4	3.7261137478196024E-5	1.65605055458649E-5	0.24549086414870802	7.038214856992583E-5
124-125	0.12716812208669656	2.463375199947404E-4	3.7261137478196024E-5	1.65605055458649E-5	0.2460456410844945	7.452227495639205E-5
126-127	0.12718261252904922	2.484075831879735E-4	3.7261137478196024E-5	1.65605055458649E-5	0.24668943073759	7.452227495639205E-5
128-129	0.1271888227186289	2.504776463812066E-4	3.7261137478196024E-5	1.65605055458649E-5	0.2473332203906855	8.07324645360914E-5
130-131	0.1272012430977883	2.5875789915413903E-4	3.7261137478196024E-5	1.65605055458649E-5	0.24806188263470358	8.28025277293245E-5
132-133	0.12721780360333415	2.608279623473722E-4	3.7261137478196024E-5	1.65605055458649E-5	0.24872430285653815	8.694265411579073E-5
134-135	0.1272260838561071	2.649680887338384E-4	3.7261137478196024E-5	1.65605055458649E-5	0.24923767852845996	8.694265411579073E-5
136-137	0.12723436410888003	2.649680887338384E-4	3.7261137478196024E-5	1.65605055458649E-5	0.24985662742323667	8.694265411579073E-5
138-139	0.12723436410888003	2.670381519270715E-4	3.7261137478196024E-5	1.65605055458649E-5	0.2505004170763322	8.694265411579073E-5
>>END_MODULE
